scholarly journals Pengaruh Nutrisi Dan Suhu Terhadap Selektivitas Potensi Antibakteri Dari Bakteri Yang Berasosiasi Dengan Spons

2018 ◽  
Vol 21 (1) ◽  
pp. 65 ◽  
Author(s):  
Tutik Murniasih ◽  
Joko Tri Wibowo ◽  
Masteria Yunovilsa Putra ◽  
Febriana Untari ◽  
Mery Maryani
Keyword(s):  
Antibacterial Activity ◽  
Yeast Extract ◽  
Sea Water ◽  
Bacterial Density ◽  
Bacterial Strains ◽  
Associated Bacteria ◽  
Direct Sampling ◽  
Microbial Symbiont ◽  
Sponge Associated Bacteria ◽  
Isolation Media

Abstract The Influence of nutrient and temperature to the  antibacterial selectivity of  Sponge Associated-Bacteria Production of pharmacological activity by marine microorganism is strongly influenced by nutrition and environmental conditions. In this study would  discuss about the influence of several type of media to the production of antibacterial agent by sponge-. associated microorganisms. About 3 sponges tissue Theonella sp, Callispongia sp. and Lithistide sp. collected from Seribu Island will be used for the host of associated microorganism. Agar medium used for isolation were M1 that contained amylum,  yeast extract and peptone, M2 (10% marine broth media) contained yeast extract and peptone, M3 only sea water without adding any nutrients. Beside the nutrient variation,  heat sock treatment at 50oC toward the sponge solution also apply to this study. The bacterial isolation data indicated that bacterial density in (CFU/100µL) of  Theonella sp, Callispongia sp. and Lithistide sp. were minimum when spreading in M3 medium with heat sock treatment. This data showed that limiting in nutrient and heating could increasing bacterial selectivity. The antibacterial activity capability of bacterial strains isolated using M1, M2 and M3 respectively in range were 81,8-90,9%; 50-87,5%  and; 66,7 -100%. This results showed that less nutrient of media will rise the number of antibacterial activity strains,and decreasing of bacterial density. This study reported that the minimum nutrient of isolation media and heat shock treatment could be used for selecting the antibacterial strains of sponge associated bacteria. Keywords : isolation media, antibacterial, nutrient, microbial symbiont AbstrakAktifitas farmakologi yang dihasilkan oleh mikroorganisme laut sangat dipengaruhi oleh nutrisi dan kondisi lingkungan. Hal tersebut mendorong untuk digali lebih dalam tentang aspek-aspek yang mempengaruhi seleksi mikroba potensial pada spons. Metode isolasi mikroba dari jaringan spons menjadi kunci dalam menguak potensi mikroba simbionnya. Dalam penelitian ini akan membahas pengaruh berbagai media isolasi bakteri yang berasosiasi dengan spons asal Kep. Seribu. Terhadap 3 specimen spons Theonella sp., Callispongia sp. dan Lithistide sp. dilakukan isolasi bakteri dengan metode direct sampling menggunakan media M1, M2 dan M3. Media M1 mengandung nutrisi antara lain amilum, ekstrak khamir dan pepton, sedangkan media M2 mengandung sumber nutrisi ekstrak khamir dan pepton dan M3 hanya media agar dan air laut. Selain variasi nutrient dalam media, perlakuan pemanasan pada suhu 50oC juga akan dilakukan terhadap larutan sampel spons sebelum dilakukan penyebaran pada media isolasi. Hasil isolasi bakteri yang diisolasi spons Theonella sp, Callispongia sp. dan Lithistide sp. menunjukkan bahwa kepadatan minimum diperoleh dengan menggunakan media M3 dengan perlakuan pemanansan. Dari data isolasi bakteri menunjukkan bahwa selain kandungan nutrient yang minimum, perlakuan pemanasan akan menurunkan kepadatan jumlah bakteri yang tumbuh, sehingga pemanasan merupakan salah satu cara dalam seleksi isolasi bakteri yang berasosiasi dengan spons. Hasil analisis aktivitas antibakteri menunjukkan bahwa persentase strain-strain bakteri yang aktif terhadap antimikroba Vibrio eltor, Eschericia coli dan Bacillus subtilis dengan variasi media M1 berkisar antara 81,8-90,9%;  M2 berkisar 50-87,5% dan M3 berkisar 66,7-100%. Dari data tersebut disimpulkan bahwa semakin sedikitnya nutrisi media isolasi maka semakin tingginya mikroba-mikroba potensial penghasil antibiotik. Media M3 merupakan media yang selektif untuk isolasi mikroba potensial dari spons, terbukti dengan tingginya prosentase bakteri yang aktif dan berkurangnya jumlah koloni yang tumbuh.Kata kunci : media isolasi, antibakteri, nutrisi, mikroba simbion

scholarly journals ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 42
Author(s):  
FRANSISCA SUNNY ◽  
TRI HANDAYANI KURNIATI ◽  
ARIANI HATMANTI
Keyword(s):  
Antibacterial Activity ◽  
Diffusion Method ◽  
Marine Agar ◽  
Antibacterial Compounds ◽  
Associated Bacteria ◽  
Antibacterial Assay ◽  
Sponge Associated Bacteria ◽  
Oxidase Test ◽  
El Tor ◽  
Produce Acid

ABSTRACT Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial  compounds.  coral  and  sponge-ssociated  bacteria  are  potential  producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and   was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with  Staphylococcus  aureus,  Bacillus  subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate  was  isolated  from  corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and  S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid.   Key words: antibacterial activity, association, characterization, coral, isolation, sponge

scholarly journals Cyclic Dipeptides Produced by Marine Sponge-Associated Bacteria as Quorum Sensing Signals

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Gennaro Roberto Abbamondi ◽  
Salvatore De Rosa ◽  
Carmine Iodice ◽  
Giuseppina Tommonaro
Keyword(s):  
Quorum Sensing ◽  
Quorum Quenching ◽  
Marine Sponges ◽  
Bacterial Strains ◽  
Signal Molecule ◽  
Associated Bacteria ◽  
Cyclic Dipeptides ◽  
Sponge Associated Bacteria ◽  
Dysidea Avara ◽  
Quorum Sensing Signals

Four bacterial strains belonging to the genera Vibrio, Pseudoalteromonas and Photobacterium were isolated from the marine sponges Dysidea avara and Geodia cynodium. A Bacillus strain was isolated from Ircinia variabilis. A screening of molecules involved in quorum sensing (QS) was carried out by TLC-overlay and a new “plate T-streak” test. To analyze quorum quenching (QQ), a plate T-streak was performed with Chromobacterium violaceum. Strains of Vibrio isolated from both marine sponges and a strain of Photobacterium isolated from G. cynodium, activated QS bioreporters. A strain of Pseudoalteromonas isolated from D. avara showed QQ activity. Finally, it is reported that cyclic dipeptides isolated from strains of Vibrio sp. and Bacillus sp. (isolated from D. avara and I. variabilis, respectively) were involved in the QS mechanism. The simultaneous presence of bacteria that showed contrasting responses in bioassays for QS signal molecule synthesis in marine sponges could add an interesting dimension to the signalling interactions which may be happening in sponges.

scholarly journals Potency of sponge-associated bacteria producing bioactive compounds as biological control of vibriosis on shrimp

2017 ◽  
Vol 16 (1) ◽  
pp. 41
Author(s):  
Adityawati Fajar Rini ◽  
Munti Yuhana ◽  
Aris Tri Wahyudi
Keyword(s):  
16S Rrna ◽  
Bioactive Compounds ◽  
Polyketide Synthase ◽  
Sea Water ◽  
White Shrimp ◽  
Pathogenicity Test ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p class="Pa5"><em>Vibrio </em>sp. is a pathogen that causes a decrease in white shrimp production. Sponge-associated bacteria are known as bioactive compounds producer in marine habitat, such as antibacteria, which can be an alternative solution in preventing vibriosis. Thus, this study was aimed to obtain a sponge-associated bacteria biocontroller to inhibit vibriosis <em>in vitro</em>, <em>in vivo </em>and also in 16S-rRNA, Non-ribosomal peptide synthase (NRPS) and polyketide synthase (PKS) genes to demonstrate its ability of synthesizing (bioactive). <em>Aaptos </em>sp. and <em>Hyrtios </em>sp. sponges were collected from Pramuka Island, Jakarta. The isolation using sea water complete (SWC) and zobel marine agar (ZMA) resulted in 174 isolates, and a total of 69 isolates were successfully screened based on their antibacterial compound activity, while a total of 47 isolates were observed to have negative hemolysis (in hemolytic test). The pathogenicity test used twelve selected isolates, that have broad spectrums of antibacteria activity and hemolysis negative, showed that the selected isolates were not pathogenic to post-larva shrimp, indicating that shrimp survival rate (pathogenicity test) did not significantly differ (P&gt;0,05) compared to the negative control. Genetic analysis based on 16S-rRNA revealed three genera groups that belonged to <em>Pseudomonas</em>, <em>Staphylococcus</em>, and <em>Alcaligenes</em>. NRPS and PKS genes detection using PCR engendered four potential bacteria isolates, which have the NRPS gene only, and one isolate having the PKS gene only and one isolate having both NRPS and PKS genes, proving that the bacteria produce bioactive compounds.</p><p> </p><p class="Pa5">Keywords: NRPS, PKS, anti-vibriosis, white shrimp</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK </strong></p><p> </p><p class="Pa5"><em>Vibrio </em>sp. merupakan salah satu bakteri patogen yang menyebabkan penurunan produksi udang. Bakteri yang berasosiasi dengan spons diketahui merupakan produsen senyawa bioaktif perairan salah satunya sebagai antibakteri. Hal ini dapat menjadi alternatif solusi dalam penanggulangan vibriosis. Penelitian ini bertujuan untuk memperoleh isolat bakteri asosiasi spons yang mempunyai kemampuan dalam menghambat vibriosis secara <em>in vitro</em>, <em>in vivo </em>dan mendeteksi gen 16S-rRNA, nonribosomal peptide synthase (NRPS) serta polyketide synthase (PKS) untuk memastikan kemampuan mensintesis senyawa bioaktif. Spons <em>Aaptos </em>sp. dan <em>Hyrtios </em>sp. berhasil dikoleksi dari perairan Pulau Pramuka, Kep. Seribu Jakarta. Isolasi bakteri dengan menggunakan media sea water complete (SWC) dan zobel marine agar (ZMA) diperoleh 174 isolat. Sebanyak 69 isolat terdeteksi memiliki aktivitas antibakteri. Uji hemolisis menunjukkan 47 isolat adalah hemolisis negatif. Uji patogenisitas menggunakan 12 isolat terpilih yang memiliki spektrum luas dan hemolisis negatif. Hasil uji patogenisitas tehadap 12 isolat menunjukkan bahwa semua isolat tidak bersifat patogen terhadap pascalarva udang vaname. Hal ini dibuktikan dengan sintasan pascalarva udang vaname yang tidak berbeda nyata (P&gt;0,05) dengan kontrol negatif. Hasil uji tantang terhadap <em>Vibrio harveyi </em>diketahui sintasan pascalarva udang vaname (70±5,0–90±0,0%) memiliki perbedaan yang signifikan jika dibandingkan dengan kontrol positif (38,3±2,9%). Berdasarkan analisis sekuen gen 16S-rRNA, menunjukkan bahwa isolat-isolat tersebut memiliki kemiripan dengan genus <em>Pseudomonas</em>, <em>Staphylococcus </em>dan <em>Alcaligenes</em>. Deteksi gen NRPS dan PKS menggunakan PCR diperoleh empat isolat bakteri memiliki hanya gen NRPS, satu isolat memiliki hanya gen PKS, dan satu isolat memiliki kedua gen NRPS-PKS. Hal ini membuktikan bahwa keenam isolat memiliki potensi sebagai penghasil senyawa bioaktif.</p><p> </p><p>Kata kunci: NRPS, PKS, antivibriosis, udang vaname</p>

scholarly journals Selection of sponge-associated bacteria with high potential for the production of antibacterial compounds

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Riyanti ◽  
Walter Balansa ◽  
Yang Liu ◽  
Abha Sharma ◽  
Sanja Mihajlovic ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Synergistic Effects ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Field Station ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria ◽  
Macrolactin A ◽  
Host Sponge ◽  
Selection Of

AbstractThe potential of sponge-associated bacteria for the biosynthesis of natural products with antibacterial activity was evaluated. In a preliminary screening 108 of 835 axenic isolates showed antibacterial activity. Active isolates were identified by 16S rRNA gene sequencing and selection of the most promising strains was done in a championship like approach, which can be done in every lab and field station without expensive equipment. In a competition assay, strains that inhibited most of the other strains were selected. In a second round, the strongest competitors from each host sponge competed against each other. To rule out that the best competitors selected in that way represent similar strains with the same metabolic profile, BOX PCR experiments were performed, and extracts of these strains were analysed using metabolic fingerprinting. This proved that the strains are different and have various metabolic profiles, even though belonging to the same genus, i.e. Bacillus. Furthermore, it was shown that co-culture experiments triggered the production of compounds with antibiotic activity, i.e. surfactins and macrolactin A. Since many members of the genus Bacillus possess the genetic equipment for the biosynthesis of these compounds, a potential synergism was analysed, showing synergistic effects between C14-surfactin and macrolactin A against methicillin-resistant Staphylococcus aureus (MRSA).

scholarly journals Bacteria Isolated From the Antarctic Sponge Iophon sp. Reveals Mechanisms of Symbiosis in Sporosarcina, Cellulophaga, and Nesterenkonia

2021 ◽  
Vol 12 ◽  
Author(s):  
Mario Moreno-Pino ◽  
Juan A. Ugalde ◽  
Jorge H. Valdés ◽  
Susana Rodríguez-Marconi ◽  
Génesis Parada-Pozo ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Marine Ecosystem ◽  
Marine Sponges ◽  
Bacterial Strains ◽  
Diverse Range ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria ◽  
Phylogenomic Analyses ◽  
The Antarctic ◽  
Antarctic Environment

Antarctic sponges harbor a diverse range of microorganisms that perform unique metabolic functions for nutrient cycles. Understanding how microorganisms establish functional sponge–microbe interactions in the Antarctic marine ecosystem provides clues about the success of these ancient animals in this realm. Here, we use a culture-dependent approach and genome sequencing to investigate the molecular determinants that promote a dual lifestyle in three bacterial genera Sporosarcina, Cellulophaga, and Nesterenkonia. Phylogenomic analyses showed that four sponge-associated isolates represent putative novel bacterial species within the Sporosarcina and Nesterenkonia genera and that the fifth bacterial isolate corresponds to Cellulophaga algicola. We inferred that isolated sponge-associated bacteria inhabit similarly marine sponges and also seawater. Comparative genomics revealed that these sponge-associated bacteria are enriched in symbiotic lifestyle-related genes. Specific adaptations related to the cold Antarctic environment are features of the bacterial strains isolated here. Furthermore, we showed evidence that the vitamin B5 synthesis-related gene, panE from Nesterenkonia E16_7 and E16_10, was laterally transferred within Actinobacteria members. Together, these findings indicate that the genomes of sponge-associated strains differ from other related genomes based on mechanisms that may contribute to the life in association with sponges and the extreme conditions of the Antarctic environment.

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

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