Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum  β-Lactamase (ESBL) E. coli

The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted using ethyl acetate. The antibacterial activity of crude extracts was tested using the paper disc diffusion method while bioactive compounds were characterized using the phytochemical method. The results showed that the antibacterial activity of the broth media extract performed a greater activity than the crude extract from the mycelium. The crude extract from mycelia only contained flavonoid and phenol hydroquinone compounds while the crude extract from broth media contains alkaloids, flavonoids, phenols hydroquinone and saponins. Kapang Trichoderma asperellum MT02 telah dilaporkan memiliki potensi sebagai penghasil senyawa antibakteri melawan Extended Spectrum β-Lactamase (ESBL) E. coli berdasarkan hasil penapisan melalui metode agar plug. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kasar kapang T. asperellum MT02 serta komposisi golongan senyawa bioaktif yang dimiliki. Kapang dikultur pada media Malt Extract Broth (MEB) (statis, 27 oC, 15 hari) di mana metabolit intraseluler dari miselium diekstrak menggunakan metanol sedangkan metabolit ekstraseluler dari media kaldu diekstrak menggunakan etil asetat. Aktivitas antibakteri ekstrak kasar diuji menggunakan metode difusi kertas cakram sedangkan senyawa bioaktif dikarakterisasi menggunakan metode fitokimia. Hasil penelitian menunjukkan bahwa aktivitas antibakteri asal ekstrak media kaldu lebih baik dibandingkan ekstrak kasar asal miselium kapang. Ekstrak kasar kapang asal miselia hanya mengandung senyawa golongan flavonoid dan fenol hidrokuinon sedangkan ekstrak kasar asal media kaldu mengandung alkaloid, flavonoid, fenol hidroquinon dan saponin.

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Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli

Jurnal Kelautan Tropis ◽

10.14710/jkt.v0i0.3528 ◽

2019 ◽

Vol 22 (1) ◽

pp. 9

Author(s):

Mada Triandala Sibero ◽

Aninditia Sabdaningsih ◽

Ocky Karna Radjasa ◽

Agus Sabdono ◽

Agus Trianto ◽

...

Keyword(s):

Antibacterial Activity ◽

Bioactive Compounds ◽

Crude Extract ◽

Diffusion Method ◽

Malt Extract ◽

Trichoderma Asperellum ◽

E Coli ◽

Extended Spectrum ◽

Agar Plug ◽

Extracellular Metabolites

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UJI AKTIVITAS ANTIBAKTERI BEBERAPA SPONS DARI PERAIRAN SALIBABU KEPULAUAN TALAUD

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.24495 ◽

2019 ◽

Vol 7 (3) ◽

pp. 234

Author(s):

Remus B Maradou ◽

Fitje Losung ◽

Remmy EP Msngindaan ◽

Rosita AJ Lintang ◽

Wilmy E Pelle ◽

...

Keyword(s):

Antibacterial Activity ◽

Coral Reefs ◽

Key Words ◽

Crude Extract ◽

Bioactive Compound ◽

Diffusion Method ◽

Water Fraction ◽

E Coli ◽

Agar Diffusion ◽

Agar Diffusion Method

Sponge is one component of coral reefs that has the potential as a bioactive compound but has not been widely used. This study was directed to obtain some spongy crude extracts from the Salibabu waters of the Talaud Islands, determine the antibacterial activity of some crude extruded sponges and compare the antibacterial activity of the sponge fraction against E. coli and S. aureus test bacteria. The results of identification of sponges were found in three species consisting of: Siphonodictyon sp., Ircinia sp., Dysidea sp. The antibacterial activity of crude extract, water, methanol and hexane fractions using agar diffusion method with E. coli and S. aureus test bacteria. The crude extract of siphonodictyon sp and the water fraction of this extract show the highest antibacterial activity. Key words: Spons, Antibacterial, E. coli. S. aureus

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UJI ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS TERHADAP Escherichia coli DAN Staphylococcus aureus SERTA POTENSINYA TERHADAP AKTIVITAS ANTI-UV

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.24459 ◽

2019 ◽

Vol 7 (3) ◽

pp. 196

Author(s):

Monika Gabrielle ◽

Deiske A Sumilat ◽

Veibe Warouw ◽

Remy EP Mangindaan ◽

Chatrien AC Sinjal ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Crude Extract ◽

Diffusion Method ◽

Water Fraction ◽

E Coli ◽

Self Defense ◽

Crude Extracts ◽

Water Fractions

Sponge organisms produce bioactive compounds that are toxic as a means of self-defense. The compound is known to have the potential as an antibacterial and anti-UV which can absorb sunlight with the potential to be used as a material for making sunscreen. The purpose of this study was to obtain antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and anti-UV activity from crude extracts and sponges fractions. The antibacterial test done by agar diffusion method (Kirby and Bauer diffusion disc) and the crude extract and the active fraction of antibacterial compounds were tested in a UV spectrophotometer to see its anti-UV activity. As a result, 4 species of sponges were extracted and partitioned into water fractions, methanol fractions, and n-hexane fractions. All samples were tested for antibacterial activity and the results showed antibacterial activity against S. aureus by Plakortis sp. in crude extract (9 mm) and water fraction (8.6 mm), Agelas sp. in crude extract (7 mm) and in E. coli bacteria shown by Plakortis sp. in crude extract (12.6 mm) and water fraction (9 mm), Liosina sp. in the water fraction (7.6 mm), Haliclona sp. in the water fraction (8 mm) and Agelassp. in crude extract (10.3 mm). Crude extracts and water fractions were tested using a UV spectrophotometer for anti-UV testing, the results showed that crude extract and all the water fractions of four species sponge could absorb UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm).Keywords: sponges, antibacterial, partition, anti-UV

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Antibacterial activity of clove (Syzygium aromaticum) and cinnamon (Cinnamomum burmannii) essential oil against extended-spectrum β-lactamase-producing bacteria

Veterinary World ◽

10.14202/vetworld.2021.2206-2211 ◽

2021 ◽

pp. 2206-2211

Author(s):

Elgio Venanda Ginting ◽

Endah Retnaningrum ◽

Dyah Ayu Widiasih

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Morphological Changes ◽

Food Products ◽

Animal Origin ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

E Coli ◽

Extended Spectrum

Background and Aim: Extended-spectrum β-lactamase (ESBL) is an enzyme produced by the family of Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae, which can hydrolyze β-lactam antibiotics, such as penicillins, cephalosporins, cephamycin, and carbapenem. ESBL-producing bacteria are widely distributed from farms to slaughterhouses until food products originating from animals are available in the market, which plays an important role as a pathway for the exposure and transmission of ESBL-producing bacteria from food products of animal origin to humans. This study aimed to determine the antibacterial activity of Syzygium aromaticum (clove) and Cinnamomum verum (cinnamon) essential oils against strains resistant to ESBL-producing E. coli and K. pneumoniae isolates. Materials and Methods: The antibacterial activity of clove and cinnamon essential oils was tested against three strains of tested bacteria using the disk diffusion method. The minimum inhibitory concentration (MIC) of clove and cinnamon essential oils was determined using the broth microdilution method. The minimum bactericidal concentration (MBC) was determined using the MIC. Morphological changes on each tested bacteria were observed through scanning electron microscopy (SEM). Results: Both essential oils exhibited inhibitory effects toward all test organisms, indicated by inhibition zones around the disk. The MIC values of clove essential oil were 0.078% (v/v) for all tested bacteria, whereas the MICs of cinnamon essential oil ranged from 0.039% (v/v) to 0.156% (v/v) for all tested bacteria. MBC values of clove and cinnamon essential oils ranged from 0.078% (v/v) to 0.156% (v/v) for all tested bacteria. There were morphological changes in each tested bacterial cell that was observed through SEM. Each tested bacteria treated with clove and cinnamon essential oils showed shrinkage and cells lysis. Conclusion: It was concluded that clove and cinnamon essential oils have emerged as effective antibacterial agents by showing high antibacterial activity against ESBL-producing E. coli and K. pneumoniae isolates, as evidenced by the inhibition zone diameter and MIC value.

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Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v8i03.9567 ◽

2017 ◽

Vol 8 (03) ◽

Author(s):

Kyoung- Sun Seo ◽

Seong Woo Jin ◽

Seongkyu Choi ◽

Kyeong Won Yun

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Methanol Extract ◽

The Other ◽

Diffusion Method ◽

E Coli ◽

Agar Diffusion ◽

Agar Diffusion Method ◽

Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2016.9.2.8 ◽

1970 ◽

Vol 9 (2) ◽

pp. 3212-3214

Author(s):

Pramod Dhakal ◽

Ankit a Achary ◽

Vedamurthy Joshi

Keyword(s):

Antibacterial Activity ◽

Azadirachta Indica ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Watery Diarrhea ◽

Diffusion Method ◽

E Coli ◽

Drug Molecules ◽

Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

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Evaluation of extended spectrum beta lactamase enzymes prevalence in clinical isolates of Escherichia coli

Microbiology Research ◽

10.4081/mr.2011.e8 ◽

2011 ◽

Vol 2 (1) ◽

pp. 8

Author(s):

Ronak Bakhtiari ◽

Jalil Fallah Mehrabadi ◽

Hedroosha Molla Agamirzaei ◽

Ailar Sabbaghi ◽

Mohammad Mehdi Soltan Dallal

Keyword(s):

Escherichia Coli ◽

Disk Diffusion ◽

Confirmatory Test ◽

Diffusion Method ◽

Multi Drug Resistance ◽

Beta Lactamase ◽

Disk Diffusion Method ◽

Extended Spectrum Beta Lactamase ◽

E Coli ◽

Extended Spectrum

Resistance to b-lactam antibiotics by gramnegative bacteria, especially Escherichia coli (E. coli), is a major public health issue worldwide. The predominant resistance mechanism in gram negative bacteria particularly E. coli is via the production of extended spectrum beta lactamase (ESBLs) enzymes. In recent years, the prevalence of b-lactamase producing organisms is increased and identification of these isolates by using disk diffusion method and no-one else is not satisfactory. So, this investigation focused on evaluating the prevalence of ESBL enzymes by disk diffusion method and confirmatory test (Combined Disk). Five hundred clinical samples were collected and 200 E. coli isolates were detected by standard biochemical tests. To performing initial screening of ESBLs was used from Disk diffusion method on E. coli isolates. A confirmation test (Combined Disk method) was performed on isolates of resistant to cephalosporin's indicators. Up to 70% isolates exhibited the Multi Drug Resistance phenotype. In Disk diffusion method, 128(64%) E. coli isolates which resistant to ceftazidime and cefotaxime while in Combined Disk, among 128 screened isolates, 115 (89.8%) isolates were detected as ESBLs producers. This survey indicate beta lactamase enzymes are playing a significant role in antibiotic resistance and correct detection of them in phenotypic test by using disk diffusion and combined Disk is essential for accurate recognition of ESBLs.

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Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Notulae Scientia Biologicae ◽

10.15835/nsb739616 ◽

2015 ◽

Vol 7 (3) ◽

pp. 272-280 ◽

Cited By ~ 2

Author(s):

Ifeoma Chinyere UGWU ◽

Madubuike Umunna ANYANWU ◽

Chidozie Clifford UGWU ◽

Ogbonna Wilfred UGWUANYI

Keyword(s):

Antibacterial Agents ◽

Tetracycline Resistance ◽

Diffusion Method ◽

Phenotypic Characterization ◽

Generic Level ◽

Klebsiella Species ◽

Phenotypic Resistance ◽

E Coli ◽

Extended Spectrum ◽

Enugu State

This study was conducted to isolate generic extended-spectrum β-lactam (ESBL)-resistant enterobacteria from pigs reared in Enugu State Southeast, Nigeria and determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 190, randomly selected, apparently healthy pigs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 2 µg/ml of cefotaxime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 46 ESBL-resistant enterobacterial isolates, 4 (8.7%) were Escherichia coli, 11 (23.9%) were Salmonella species, while 31 (67.4%) were Klebsiella species. Resistance of the Salmonella isolates was 45.5% to ciprofloxacin, 36.4% to ofloxacin and levofloxacin, 9.1% to norfloxacin, amikacin and gentamicin, 27.3% to streptomycin, 72.7% to chloramphenicol and 90.9% to tetracycline. Resistance of the Klebsiella isolates was 93.5% to ampicillin, 12.9% to ciprofloxacin, 19.4% to ofloxacin and levofloxacin, 9.7% to norfloxacin and streptomycin, 64.5% to chloramphenicol and 38.7% to tetracycline. Resistance of the E. coli isolates was 100% to gentamicin, 75% to ampicillin and streptomycin, 50% to ciprofloxacin, norfloxacin, chloramphenicol and tetracycline, and 25% to ofloxacin, levofloxacin and amikacin. All the isolates were resistant to ceftriaxone, cefotaxime, ceftazidime, cefepime, cefpodoxime, amoxicillin/clavulanic acid and aztreonam. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 54.8% for Klebsiella, 90.9% for Salmonella and 100% for E. coli, respectively. This study has shown that pigs reared in Enugu State Southeast, Nigeria, are colonized by ESBL-resistant Enterobactericeae and are potential reservoirs and disseminators of these organisms.

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STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS

Wiadomości Lekarskie ◽

10.36740/wlek202109115 ◽

2021 ◽

Vol 74 (9) ◽

pp. 2109-2111

Author(s):

Evheniia A. Shtaniuk ◽

Oleksandra O. Vovk ◽

Larisa V. Krasnikova ◽

Yuliia I. Polyvianna ◽

Tetiana I. Kovalenko

Keyword(s):

Antibacterial Activity ◽

Water Soluble ◽

Diffusion Method ◽

E Coli ◽

Mueller Hinton ◽

Microbiological Methods ◽

Pure Cultures ◽

Inflammatory Processes ◽

Definition Of

The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them. Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method (“well” method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis. Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine. Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.

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Antibacterial Activity of Ethanol Extract of Spirulina platensis

Current Biochemistry ◽

10.29244/cb.7.2.1 ◽

2021 ◽

Vol 7 (2) ◽

pp. 47-51

Author(s):

Prayoga Pannindrya ◽

Mega Safithri ◽

Kustiariyah Tarman

Keyword(s):

Antibacterial Activity ◽

Spirulina Platensis ◽

Ethanol Extract ◽

Positive Control ◽

Diffusion Method ◽

Test Results ◽

Nutritional Content ◽

Disc Diffusion Method ◽

Antibacterial Test ◽

E Coli

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.

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