scholarly journals Within- and between-subject biological variation data for serum zinc, copper and selenium obtained from 68 apparently healthy Turkish subjects

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Abdurrahman Coşkun ◽  
Anna Carobene ◽  
Aasne K. Aarsand ◽  
Fehime B. Aksungar ◽  
Mustafa Serteser ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Serum Zinc ◽  
Population Based ◽  
Biological Variation ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Essential Components ◽  
Serum Zn ◽  
Males And Females ◽  
Variance Homogeneity

Abstract Objectives Trace elements (TrEL) are nutritionally essential components in maintaining health and preventing diseases. There is a lack of reliable biological variation (BV) data for TrELs, required for the diagnosis and monitoring of TrEL disturbances. In this study, we aimed to provide updated within- and between-subject BV estimates for zinc (Zn), copper (Cu) and selenium (Se). Methods Weekly serum samples were drawn from 68 healthy subjects (36 females and 32 males) for 10 weeks and stored at −80 °C prior to analysis. Serum Zn, Cu and Se levels were measured using inductively-coupled plasma mass spectrometry (ICP-MS). Outlier and variance homogeneity analyses were performed followed by CV-ANOVA (Røraas method) to determine BV and analytical variation estimates with 95% CI and the associated reference change values (RCV) for all subjects, males and females. Results Significant differences in mean concentrations between males and females were observed, with absolute and relative (%) differences for Zn at 0.5 μmol/L (3.5%), Cu 2.0 μmol/L (14.1%) and Se 0.06 μmol/L (6.0%). The within-subject BV (CVI [95% CI]) estimates were 8.8% (8.2–9.3), 7.8% (7.3–8.3) and 7.7% (7.2–8.2) for Zn, Cu and Se, respectively. Within-subject biological variation (CVI) estimates derived for male and female subgroups were similar for all three TrELs. Marked individuality was observed for Cu and Se. Conclusions The data of this study provides updated BV estimates for serum Zn, Cu and Se derived from a stringent protocol and state of the art methodologies. Furthermore, Cu and Se display marked individuality, highlighting that population based reference limits should not be used in the monitoring of patients.

Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Bhagavathami Meenaksh ◽  
Chitraa R. Chandran ◽  
Aravindhan Thathchari Ranganathan ◽  
Kavindapadi Venkateswaran Rama ◽  
Valarmathy Srinivasan
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Gingival Crevicular Fluid ◽  
Serum Zinc ◽  
Cross Sectional Study ◽  
Serum Copper ◽  
Cross Sectional ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Copper Zinc

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

scholarly journals European Biological Variation Study (EuBIVAS): within- and between-subject biological variation estimates for serum thyroid biomarkers based on weekly samplings from 91 healthy participants

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Michela Bottani ◽  
Aasne K. Aarsand ◽  
Giuseppe Banfi ◽  
Massimo Locatelli ◽  
Abdurrahman Coşkun ◽  
...  
Keyword(s):  
Large Scale ◽  
Thyroid Stimulating Hormone ◽  
Biological Variation ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Free Triiodothyronine ◽  
Performance Specifications ◽  
Study Population ◽  
Variance Homogeneity ◽  
Healthy Participants

Abstract Objectives Thyroid biomarkers are fundamental for the diagnosis of thyroid disorders and for the monitoring and treatment of patients with these diseases. The knowledge of biological variation (BV) is important to define analytical performance specifications (APS) and reference change values (RCV). The aim of this study was to deliver BV estimates for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), thyroglobulin (TG), and calcitonin (CT). Methods Analyses were performed on serum samples obtained from the European Biological Variation Study population (91 healthy individuals from six European laboratories; 21–69 years) on the Roche Cobas e801 at the San Raffaele Hospital (Milan, Italy). All samples from each individual were evaluated in duplicate within a single run. The BV estimates with 95% CIs were obtained by CV-ANOVA, after analysis of variance homogeneity and outliers. Results The within-subject (CV I ) BV estimates were for TSH 17.7%, FT3 5.0%, FT4 4.8%, TG 10.3, and CT 13.0%, all significantly lower than those reported in the literature. No significant differences were observed for BV estimates between men and women. Conclusions The availability of updated, in the case of CT not previously published, BV estimates for thyroid markers based on the large scale EuBIVAS study allows for refined APS and associated RCV applicable in the diagnosis and management of thyroid and related diseases.

scholarly journals Trace elements in patients with aortic valve sclerosis

2021 ◽  
Vol 15 ◽  
pp. 175394472098598
Author(s):  
Hataw Al-Taesh ◽  
Abuzer Çelekli ◽  
Murat Sucu ◽  
Seyithan Taysi
Keyword(s):  
Trace Elements ◽  
Aortic Valve ◽  
Inductively Coupled Plasma ◽  
Healthy Subjects ◽  
Serum Zinc ◽  
Serum Levels ◽  
Control Group ◽  
Aortic Valve Sclerosis ◽  
Inductively Coupled ◽  
Significant Difference

Background: Aortic valve sclerosis (AVSc) is defined as the thickening and calcification of aortic valve cusps, in the absence of obstruction of ventricular outflow. AVSc is linked with a clear imbalance in some trace elements. Aims: The objective of this study was to investigate the relationship between AVSc and serum levels of iron (Fe), zinc (Zn), selenium (Se), and copper (Cu). Additionally, this research aimed to explore the clinical significance of human serum zinc, selenium, copper, and iron concentrations as a potential new biomarker for AVSc patients and to clarify the pathophysiological role in individuals at risk of developing AVSc. Patients and methods: The study included 40 subjects with AVSc (25% male and 75% female) who were compared with a healthy control group with the same gender ratio. AVSc was based on comprehensive echocardiographic assessments. Blood samples were taken and Zn and Cu concentrations were determined through the use of atomic absorption spectroscopy. Se was measured using an inductively coupled plasma mass spectrometry device and Fe was measured using a Beckman Coulter instrument. Results: There was a significant difference in the prevalence of diabetes, blood pressure levels, and body mass index between the patients and the healthy subjects ( p < 0.05). The differences between the serum Fe, Se, and Cu levels of the AVSc patients and the healthy subjects ( p > 0.05) were recorded. The serum Zn of AVSc patients when compared was significantly lower compared with that of the control group ( p < 0.01). Conclusion: Patients with AVSc had an imbalance in some of the trace elements in their blood. The patient group’s valves had higher serum Cu levels and lower serum Se, Zn, and Fe concentrations compared with the healthy group’s valves. In the valve patients as compared, AVSc had a high prevalence of obesity, hypertension, and diabetes.

scholarly journals Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 465 ◽  
Author(s):  
Diego Luna ◽  
Marta López-Alonso ◽  
Yolanda Cedeño ◽  
Lucas Rigueira ◽  
Víctor Pereira ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Toxic Elements ◽  
Serum Samples ◽  
Element Analysis ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Essential And Toxic Elements ◽  
High Concentrations

This study was designed to evaluate the influence of type of blood sample (serum or plasma) on essential and toxic element analysis in cattle. Paired plasma and serum samples (n = 20) were acid digested, and the concentrations of As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn. Mo, Ni, P, Pb, Sb, Se, Sr and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). The study findings indicate that plasma and serum samples appear suitable and interchangeable for the determination of most of the essential and toxic elements in blood in cattle. The only exceptions are Cu and Se, the concentrations of which were significantly lower (40.9 and 29.9% respectively) in serum than in plasma. Some of the Cu in blood samples from bovine ruminants is known to be sequestered during clotting. However, further research on Se in ruminants and other animal species is warranted. Finally, the significantly higher Mn (9.9%) concentrations in serum than in plasma may have been caused by haemolysis of some samples. Special attention should be paid to preventing haemolysis of samples during collection and processing, in order to prevent overestimation of elements present at high concentrations inside erythrocytes (i.e., Fe, Mn and Zn).

6. Measuring isotopes

2016 ◽  
Author(s):  
Rob Ellam
Keyword(s):  
Inductively Coupled Plasma ◽  
Isotope Ratio ◽  
Ion Source ◽  
Routine Laboratory ◽  
Mass Filter ◽  
Mass Spectrometers ◽  
Inductively Coupled ◽  
Gas Source ◽  
Essential Components ◽  
Secondary Ion

Mass spectrometers have become routine laboratory instruments in many disciplines. ‘Measuring isotopes: mass spectrometers’ concentrates on those used to quantify the abundance of different isotopes—gas source isotope ratio, thermal ionization, inductively coupled plasma, and secondary ion mass spectrometers. A mass spectrometer can be used to quantify the concentration of a particular element by monitoring an isotope of that element not overlapped by isotopes of other elements. All mass spectrometers have three essential components: an ion source, a mass filter, and a detector. There are two main types of detector: Faraday detectors measure large signals and a variant of photomultiplier tubes measures small isotope signals.

scholarly journals Determinants of Erythrocyte Lead Levels in 454 Adults in Florence, Italy

2019 ◽  
Vol 16 (3) ◽  
pp. 425 ◽  
Author(s):  
Saverio Caini ◽  
Benedetta Bendinelli ◽  
Giovanna Masala ◽  
Calogero Saieva ◽  
Melania Assedi ◽  
...  
Keyword(s):  
Urban Areas ◽  
Inductively Coupled Plasma ◽  
Dietary Habits ◽  
Hormone Replacement ◽  
Body Burden ◽  
Population Based ◽  
Linear Regression Models ◽  
Inductively Coupled ◽  
Adverse Health Effects ◽  
Lead Levels

Background: Lead exposure, even at low levels, is associated with adverse health effects in humans. We investigated the determinants of individual lead levels in a general population-based sample of adults from Florence, Italy. Methods: Erythrocyte lead levels were measured (using inductively coupled plasma-mass spectrometry) in 454 subjects enrolled in the Florence cohort of the European Prospective Investigation into Cancer and Nutrition (EPIC) study in 1992–1998. Multiple linear regression models were used to study the association between demographics, education and working history, lifestyle, dietary habits, anthropometry, residential history, and (among women) menstrual and reproductive history and use of exogenous sex hormones, and erythrocyte lead levels. Results: Median lead levels were 86.1 μg/L (inter-quartile range 65.5–111.9 μg/L). Male gender, older age, cigarette smoking and number of pack-years, alcohol intake, and residing in urban areas were positively associated with higher erythrocyte lead levels, while performing professional/managerial or administrative work or being retired was inversely associated with lead levels. Among women, lead levels were higher for those already in menopause, and lower among those who ever used hormone replacement therapy. Conclusions: Avoidable risk factors contribute to the lead body burden among adults, which could therefore be lowered through targeted public health measures.

scholarly journals Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Jaimie M. Strickland ◽  
Doug Lyman ◽  
Lorraine M. Sordillo ◽  
Thomas H. Herdt ◽  
John P. Buchweitz
Keyword(s):  
Oxidative Stress ◽  
Liver Disease ◽  
Dairy Cows ◽  
Inductively Coupled Plasma ◽  
Copper Concentration ◽  
Tissue Level ◽  
Copper Accumulation ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Hepatic Copper

Concerns regarding excessive hepatic copper concentrations in dairy cows have increased. The objective of this study was to determine the association of hepatic copper concentrations with evidence of liver disease. Blood and liver samples were collected at the time of slaughter in cull dairy cows (n=100). Liver samples were analyzed for copper using inductively coupled plasma mass spectrometry and crude fat using liquid-liquid extraction and gravimetry. Serum samples were analyzed for glutamate dehydrogenase,γ-glutamyltransferase, sorbitol dehydrogenase, aspartate aminotransferase activities, and bile acid concentrations. Liver samples were examined histologically for inflammation, fibrosis, and rhodanine staining. Animals were stratified by hepatic copper concentration and samples in the highest and lowest quintiles (Q5 and Q1) were evaluated for oxidative stress. Systemic indices of oxidative stress included serum reactive oxygen and nitrogen species (RONS) and total antioxidant potential (AOP). Tissue-level oxidative stress was assessed by immunohistochemistry using 4-hydroxynonenal (4HNE) and 3-nitrotyrosine (3NIT) stains to score the relative abundance and distribution of oxidized lipid and protein products, respectively. Mean hepatic copper concentration was 496.83μg/g and median 469.72μg/g and ranged from 70.56 to 1264.27μg/g dry tissue. No association was found between hepatic copper concentrations and clinicopathological or histological evidence of hepatic damage or dysfunction. There was a significant increase in the amount of IHC staining of 4HNE and 3NIT in Q5 compared with Q1. Moreover, the IHC staining mirrored the distribution of the copper-specific stain rhodanine. These results demonstrate that cows with elevated hepatic copper concentrations had no evidence of active liver disease but had increased hepatic oxidative stress.

Serum Selenium Levels in Glaucoma: a Pilot Study

2021 ◽  
Author(s):  
Marianna Lucio ◽  
Alexander Fick ◽  
Bernhard Michalke ◽  
Robert Laemmer ◽  
Sami Hosari ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Open Angle Glaucoma ◽  
Study Groups ◽  
Gender Effects ◽  
Total Serum ◽  
Serum Selenium ◽  
Serum Samples ◽  
Age And Gender ◽  
Inductively Coupled ◽  
And Gender

Abstract Background Trace elements are assumed to be involved in glaucoma pathogenesis via changes in oxidative stress. Especially serum selenium (Se) has been linked to this neurodegenerative disease. Serum Se levels differ between countries due to nutrition and ethnicity. It was the aim of the present study to investigate serum Se levels in primary open-angle glaucoma (POAG) patients and controls in Germany and to consider potential age and gender effects. Material and Methods The Se concentration of 39 serum samples (22 patients with POAG, 17 controls) were analyzed by inductively coupled plasma-sector field mass spectrometry (ICP-sf-MS) in high resolution mode. Covariance and percentile regression were analyzed. Age and gender were defined as confounding factors and their different trends were investigated. Moreover, age was examined across different quantiles of Se levels. Results Total serum least-squares means (LS-means) Se levels were 132.02 µg/L (controls) and 134.86 µg/L (POAG). Total serum Se levels did not differ between the study groups (p > 0.05). Significant age and gender effects of serum Se were observed. Quantile analysis showed that the 1st serum Se quantile decreased with increasing age in POAG patients in contrast to controls. The odds ratios of the 1st serum Se were 1.3 (with 2nd quantile) and 1.3 (with 3rd quantile), respectively. Conclusion The serum Se level of the German cohort was almost half of those of the published US cohort (glaucoma 209.11 ng/mL; control 194.45 ng/mL). Age and gender effects were observed; the serum Se level increased with age in women (controls and POAG), however, Se levels decreased with age in men (controls and POAG).

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