scholarly journals Effect of Nanoaquacitrates on Physiological Parameters of Fodder Galega Infected with Phytoplasma

2018 ◽  
Vol 25 (1) ◽  
pp. 153-168 ◽  
Author(s):  
Hanna Huliaieva ◽  
Iryna Tokovenko ◽  
Victor Maksin ◽  
Volodymyr Kaplunenko ◽  
Antonina Kalinichenko
Keyword(s):  
Chlorophyll Content ◽  
Laboratory Experiments ◽  
Photosynthetic Apparatus ◽  
Photochemical Activity ◽  
Electron Acceptors ◽  
Negative Effects ◽  
Bisphosphate Carboxylase ◽  
Oxygenase Activity ◽  
Foliar Treatment ◽  
Galega Orientalis

Abstract The laboratory experiments have been found that soaking seeds Galega orientalis L. (Fodder galega) in nanoaquacitrates solutions of Mn (10 and 20 mg/dm3), Mo (4 mg/dm3) and Mg (2 and 4 mg/dm3), has been lead to germination energy rise, while Mn (10 and 20 mg/dm3) and Mo (4 mg/dm3) concentrations has been influenced germinating ability. At the same time, the soaking seeds in solution of nanoaquacitrates Mn (20 mg/dm3) had the biggest stimulatory effect on the accumulation 7 daily sprouts mass (on 18%). It has been shown that soaking seeds in nanoparticles Mn and Mo solutions leads to the increase of catalase activities (especially under the influence of manganese) and peroxidase activities (under molybdenum influence). Applying the method of chlorophyll a fluorescence in the field and greenhouse experiments with Galega orientalis L. plants, artificial infected with phytoplasma Acholeplasma laidlawii var. granulum st. 118 the following changes in the photosynthetic apparatus has been indicated: reduction in the length of the light-antenna, blocking transport of electrons in plastoquinone pool PSII with reducing the pool of electron acceptors. It has also been indicated that photochemical activity resistance of the photosynthetic apparatus decreases while its stability increases, as result of described above effects the concentration of chlorophyll a and b in plants leaves decreases. The above-mentioned negative effects have been deactivated through foliar treatment of infected Galega orientalis L. plants with nanoaquacitrates solution Mo (4 mg/dm3) that allow increasing of photochemical resistance of photosynthetic apparatus as well as chlorophyll content in leaves. The foliar treatment with Mn (20 mg/dm3) solution of the infected plants, in compared with infected plants without treatment, resulted in more significant increase of Ki value (which correlate to the ribulose-1,5-bisphosphate carboxylase/oxygenase activity), which is explaining anti-mycoplasma effect of this solution.

scholarly journals Peculiarities of pigment complex functioning of winter wheat plants depending on the fertilizer application method

2021 ◽  
Vol 12 (3) ◽  
pp. 7-16
Author(s):  
Z. Bilousova ◽  
◽  
V. Keneva ◽  
Y. Klipakova ◽  
◽  
...  
Keyword(s):  
Winter Wheat ◽  
Magnesium Sulfate ◽  
Chlorophyll Content ◽  
Photosynthetic Apparatus ◽  
Fertilizer Application ◽  
Pigment Content ◽  
Plant Leaves ◽  
Significant Difference ◽  
Foliar Treatment ◽  
Pigment Complex

To obtain the maximum yield of winter wheat, it is necessary to further optimize the existing cultivation technologies in the direction of their adaptation to changing environmental conditions. One of the areas of adaptation of plants to adverse abiotic factors is the active functioning of the photosynthetic apparatus, which depends on the amount of nutrients introduced. The influence of fertilizer application on the condition of the pigment complex of winter wheat plants in the conditions of the Southern Steppe of Ukraine has been studied. Two varieties of winter wheat were selected for the study: Shestopalivka and Mason. The experiment scheme involved the application of fertilizers at sowing (K0; K12) and foliar treatment with various tank mixtures (urea; urea + magnesium sulfate; urea + magnesium sulfate + potassium monophosphate). The pigment content has been determined by grinding fresh leaves of winter wheat, followed by the addition of a solvent in the form of acetone. Measurements of pigments were performed using a spectrophotometer. According to the research results, it was established that before the foliar treatment the a-chlorophyll content and carotenoids was higher in the plant leaves of the Shestopalivka variety. At the same time, the b-chlorophyll content on the contrary was higher for plants of the Mason variety by 17%, which may be due to the adaptation of plants of this variety to lack of light. On the 3rd day after foliar treatment, a decrease in the pigment content in the plant leaves of all experimental variants has been observed, which was due to the active growth of the photosynthetic surface and a decrease in the total dry matter mass. There was no significant difference between the varieties of the content of photosynthetic pigments in this period. On the 10th day after foliar treatment, an increase in a- and b-chlorophyll content has been observed for both studied varieties, which may be the result of adaptation of the photosynthetic apparatus of winter wheat plants to lighting conditions. Foliar treatment of winter wheat plants with a tank mixture of urea with magnesium sulfate and potassium monophosphate contributed to a further increase in the content of a-chlorophyll by 12-23%, and b-chlorophyll by 5-37% depending on the variety compared to the control. The results of the research testify to the high efficiency of complex application of nitrogen-phosphorus-potassium fertilizers for foliar treatment of winter wheat plants in the BBCH 31 stage, both against the background of pre-sowing application of potassium fertilizers and without it.

scholarly journals Inhibition or Facilitation? Contrasted Inter-Specific Interactions in Sphagnum under Laboratory and Field Conditions

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1554
Author(s):  
Chao Liu ◽  
Zhao-Jun Bu ◽  
Azim Mallik ◽  
Yong-Da Chen ◽  
Xue-Feng Hu ◽  
...  
Keyword(s):  
Field Experiment ◽  
Species Interactions ◽  
Laboratory Experiments ◽  
Resource Competition ◽  
Specific Interactions ◽  
Interaction Type ◽  
Negative Effects ◽  
Experiment Data ◽  
The Stability ◽  
Positive Effect

In a natural environment, plants usually interact with their neighbors predominantly through resource competition, allelopathy, and facilitation. The occurrence of the positive effect of allelopathy between peat mosses (Sphagnum L.) is rare, but it has been observed in a field experiment. It is unclear whether the stability of the water table level in peat induces positive vs. negative effects of allelopathy and how that is related to phenolic allelochemical production in Sphagnum. Based on field experiment data, we established a laboratory experiment with three neighborhood treatments to measure inter-specific interactions between Sphagnum angustifolium (Russ.) C. Jens and Sphagnum magellanicum Brid. We found that the two species were strongly suppressed by the allelopathic effects of each other. S. magellanicum allelopathically facilitated S. angustifolium in the field but inhibited it in the laboratory, and relative allelopathy intensity appeared to be positively related to the content of released phenolics. We conclude that the interaction type and intensity between plants are dependent on environmental conditions. The concentration of phenolics alone may not explain the type and relative intensity of allelopathy. Carefully designed combined field and laboratory experiments are necessary to reveal the mechanism of species interactions in natural communities.

scholarly journals Improvement of the photosynthetic activity of Moldavian dragonhead (Dracocephalum moldavica L.) through foliar application of a nitrophenolate–based biostimulant

2018 ◽  
Vol 10 ◽  
pp. 01009
Author(s):  
Sławomir Kocira ◽  
Agnieszka Sujak ◽  
Tomasz Oniszczuk ◽  
Agnieszka Szparaga ◽  
Mariusz Szymanek ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Photosynthetic Activity ◽  
Foliar Application ◽  
Positive Impact ◽  
Abiotic Stress Tolerance ◽  
Photosynthetic Apparatus ◽  
Nutrient Content ◽  
Physiological Processes ◽  
Naturally Occurring ◽  
Reported Study

Application of biostimulants instigates many physiological processes that enhance nutrition efficiency, abiotic stress tolerance, and quality traits of crops, regardless of their nutrient content. One of such preparations is Atonik which contains nitrophenol compounds naturally occurring in plant cells. Several studies have confirmed its beneficial effect on the growth, development, and improved metabolic activity of plants. Therefore, it seems advisable to investigate the effect of Atonik preparation on the photosynthetic activity of Moldavian dragonhead (Dracocephalum moldavica L.). The reported study was carried out in 2014 in Perespa, Poland. Over the growing season, Atonik was foliar-applied at a dose of 0.3 L/ha (0.1%) and 0.6 L/ha (0.2%) by single and double spraying of plants. Chlorophyll content and nitrogen status (N) were estimated by a Chlorophyll Meter SPAD-502 Plus. The foliar application of Atonik was found to improve the efficiency of the photosynthetic apparatus and chlorophyll content in the leaves of Dracocephalum moldavica plants, but the results were dependent on biostimulant concentration and number of its applications, and on the date of measurement. To conclude, Atonik is an environmental-friendly preparation which has a positive impact on the metabolic processes of plants.

Self-Interested Giving: The Relationship Between Conditional Gifts, Charitable Donations, and Donor Self-Interestedness

2021 ◽  
Author(s):  
Matthew Chao ◽  
Geoffrey Fisher
Keyword(s):  
Decision Analysis ◽  
Experimental Evidence ◽  
Laboratory Experiments ◽  
Self Report ◽  
Negative Effects ◽  
Charitable Donations ◽  
Prosocial Motives ◽  
The Gift ◽  
Other Regarding ◽  
The Relationship

Nonprofits regularly use conditional “thank you” gifts to entice prospective donors to give, yet experimental evidence suggests that their effects are mixed in practice. This paper uses multiple laboratory experiments to test when and why thank you gifts vary in effectiveness. First, we demonstrate that although gifts often increase donations to charities that donors did not rate highly, many of the same gifts had no effects or negative effects for charities that prospective donors already liked. We replicate these findings in a second experiment that uses a different range of charity and gift options as well as different measures of participant perceptions of a charity. We also find that making gifts optional, as is common in fundraising campaigns, does not eliminate these negative gift effects. In additional experiments, we directly test for donor motives using self-report and priming experiments. We find that thank you gifts increase (decrease) the weight that donors place on self-interested (prosocial) motives, leading to changes in donation patterns. Altogether, our results suggest that practitioners may find gifts more useful when appealing to donors not already familiar with or favorably inclined to their charity, such as during donor acquisition campaigns. They may be less useful when appealing to recent donors or others who already favor the charity, in part because the gift may activate mindsets or norms that emphasize self-interested motives instead of more prosocial, other-regarding motives. This paper was accepted by Yan Chen, decision analysis.

scholarly journals Gas exchanges in sugar apple (Annona squamosa L.) subjected to salinity stress and nitrogen fertilization

2019 ◽  
pp. 1959-1966
Author(s):  
Francisco Romário Andrade Figueiredo ◽  
Anderson Carlos de Melo Gonçalves ◽  
João Everthon da Silva Ribeiro ◽  
Toshik Iarley da Silva ◽  
Jackson Silva Nóbrega ◽  
...  
Keyword(s):  
Experimental Design ◽  
Gas Exchange ◽  
Chlorophyll Content ◽  
Irrigation Water ◽  
Nitrogen Fertilization ◽  
Annona Squamosa ◽  
Negative Effects ◽  
Chlorophyll Content And Fluorescence ◽  
Fluorescence Parameters ◽  
Salinity Levels

Salinity is one of the modern agriculture major obstacles, causing several physiological disturbances in plants, adversely affecting its growth and development. Therefore, some techniques are required in order to alleviate the negative effects of salinity stress on plants, as for example an adequate nitrogen fertilization. The aim of this study was to assess the ecophysiological responses of sugar apple plants (Annona squamosa L.) submitted to different salinity levels and nitrogen fertilization doses. The experimental design was a randomized block in an incomplete factorial scheme, with five electrical conductivities of the irrigation water (ECw: 0.5, 1.01, 2.25, 3.49 and 4.0 dS m-1) and five nitrogen doses (0, 101, 350, 598.2 and 700 mg dm-3), with four replicates, generated from the Box Central Composite experimental design matrix. Through a daily course, it was observed variations in gas exchange, chlorophyll content and fluorescence parameters. The gas exchange, chlorophyll content and fluorescence parameters were measured on photosynthetically active leaves. There was significant interaction between the salinity levels (ECw) and nitrogen doses for the chlorophyll indexes. The electrical conductivity of the irrigation water caused significant negative effects on chlorophyll fluorescence. Therefore, it can be stated that the sugar apple plants ecophysiology varies through the day and that salinity affects its chlorophyll content and fluorescence.

Changes in photosynthetic apparatus of mustard (Sinapis alba) cotyledons following cycloheximide and kinetin treatments

Biologia ◽  
2008 ◽  
Vol 63 (5) ◽  
Author(s):  
Adriana Machlicová ◽  
L’udmila Slováková ◽  
Ján Hudák
Keyword(s):  
Photosynthetic Pigments ◽  
Structural Characteristics ◽  
Photosynthetic Apparatus ◽  
Chloroplast Ultrastructure ◽  
Membrane System ◽  
Sinapis Alba ◽  
Negative Effects ◽  
Chlorophyll Pigments ◽  
Leaf Mesophyll ◽  
Rubisco Subunits

AbstractBiochemical and accompanying structural characteristics of the photosynthetic process were studied in mustard seedlings cultivated on medium with increasing concentrations of cycloheximide alone as well as in combination with various kinetin concentrations. After 7 days of cultivation the contents of total chlorophyll, carotenoids and content of Rubisco in mustard cotyledons were determined. The content of chlorophyll pigments and carotenoids decreased in dependence of cycloheximide concentration. Following antibiotic treatment the content of both Rubisco subunits markedly decreased. In addition cycloheximide caused disturbance in mesophyll organization and chloroplast ultrastructure.Kinetin applied with cycloheximide increased the amount of photosynthetic pigments as well as of Rubisco, compared to the cycloheximide alone. In the seedlings treated with cycloheximide+kinetin the structure of leaf mesophyll and chloroplast membrane system was similar to control. Our results indicate that kinetin diminished the negative effects of cycloheximide on photosynthetic pigments and Rubisco as well as on the structural traits of the cotyledons.

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