scholarly journals Effect of Temperature Shock on the Triploidization Success Rate of Seurukan Fish (Osteochilus vittatus)

Author(s):  
Kartini Eriani ◽  
Alfis Syahrin ◽  
Zainal Abidin Muchlisin

<p>Seurukan fish (<em>Osteochilus vittatus</em>) has many advantages, besides the fish also has disadvantages which are the slow growth, so the temperature shock of triploidization technique was expected to solve the problem.   The objective of the present study was to obtain an effective temperature to increase of triploidization success of <em>seurukan</em> fish (<em>Osteochilus vittatus</em>). The experimental method and completely randomized design model were used in this study. Five levels of temperature shocks at three replicates were tasted: 4℃ (cold), 6℃ (cold), 28℃ (normal), 35℃ (heat) and 37℃ (heat). The sperms and eggs were fertilized in the plastic jar then a total 100 of fertilized eggs (zygotes) were taken randomly 3 minutes after fertilization and soaked in respective temperature for 90 seconds, and then incubated in incubation jars at the water temperature of 28-29℃. The results showed that the temperature shock gave the significant effect on the hatching and the success of triploidization success (P˂0.05), but did not give the significant effect the fertility and survival rates (P&gt;0.05). The triploid fish can be achieved using cold and heat shock, but the higher triploid fish was recorded at 37℃ was the best temperature recommended for triploidization of <em>Seurukan</em> fish.</p>

2020 ◽  
Vol 3 (1) ◽  
pp. 37
Author(s):  
Anny Rimalia

<p><em>Species, temperature shock duration, fertilization temperature from different climates can cause different hatching results, so it is necessary to do research on the treatment of variations in heat shock at the same temperature, to obtain information about the hatching rate (HR) of African catfish (Clarias gariepinus). The method used is completely randomized design (3x3). The results of the egg hatching rate (HR), with variations in the heat shock at the same temperature (35⁰C), </em><em>showed the highest rate of hatching dumbo catfish eggs at heat shock one minute 233.00%, then heat shock two minutes 212.00%, and heat shock three minutes 200.00% respectively.</em><em></em></p>


2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Christo V.S Aer ◽  
Winda M Mingkid ◽  
Ockstan J Kalesaran

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.


2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Christo V S Aer ◽  
Winda M Mingkid ◽  
Ockstan J Kalesaran

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.


Author(s):  
Ayi Yustiati ◽  
Syakirah Imtinan Zurwana ◽  
Achmad Rizal ◽  
Yuli Andriani

The purpose of this research is to determine the optimal dose of the addition of red spinach powder to artificial feed on the brightness of clown loach. This research was conducted at Aquaculture Laboratory Building 4 Faculty of Fisheries and Marine Sciences of Universitas Padjadjaran from April to May 2020. This research method is experimental with a Completely Randomized Design consisting of four treatments and three replications. The red spinach powder addition treatment used 0%, 2%, 4%, and 6% of the feed amount. The parameters observed are color value as primary data by using Toca Color Finder, while the growth, survival rate, and water quality as support data. Color assessment results were analyzed using the Kruskal-Wallis test, if there were significant differences, Z test would be performed. Growth data and survival rates were analyzed using Analysis of Variance (ANOVA). Because the F test results were not significantly different, the Duncan test was not carried out. The results showed that the addition of  6% red spinach powder is the best treatment with an increased color brightness value of 5,63.


2018 ◽  
Vol 32 (3) ◽  
pp. 347-351 ◽  
Author(s):  
Shaun M. Sharpe ◽  
Nathan S. Boyd ◽  
Peter J. Dittmar ◽  
Greg E. MacDonald ◽  
Rebecca L. Darnell

AbstractStrawberry is an important horticultural crop in Florida. The long growing season and escapes from fumigation and PRE herbicides necessitate POST weed management to maximize harvest potential and efficiency. Alternatives to hand-weeding are desirable, but clopyralid is the only broadleaf herbicide registered for use. Weed control may be improved by early-season clopyralid applications, but at risk of high temperature and increased strawberry injury. The effect of temperature on clopyralid safety on strawberry is unknown. We undertook a growth chamber experiment using a completely randomized design to determine crop safety under various temperature conditions across acclimation, herbicide application, and post-application periods. There was no effect of clopyralid on the number of strawberry leaves across all temperatures. Damage to the strawberry manifested as leaf malformations. Acclimation temperatures affected clopyralid-associated injury (p=0.0309), with increased leaf malformations at higher temperatures (27 C) compared to lower (18 C) temperatures. Pre-treatment temperatures did not affect clopyralid injury. Post-application temperature also affected clopyralid injury (p=0.0161), with increased leaf malformations at higher temperatures compared to lower ones. Clopyralid application did not reduce flowering or biomass production in the growth chamber. If leaf malformations are to be avoided, consideration to growing conditions prior to application is advisable, especially if applying clopyralid early in the season.


2004 ◽  
Vol 47 (4) ◽  
pp. 537-544 ◽  
Author(s):  
Ana Paula de Moraes ◽  
Maria Helena Bonadese-Zanettini ◽  
Sídia Maria Callegari-Jacques ◽  
Eliane Kaltchuk-Santos

In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.


Author(s):  
Ega Puspita Famila Boden Junaedi Putri ◽  
Salnida Yuniarti Lumbessy ◽  
Nanda Diniarti

Catfish (Clarias gariepinus.) is one of the leading commodities that are widely cultivated in Indonesia. The current development of the aquaculture industry, which leads to intensive cultivation systems with an increase in stocking and high amount of feed, causes an increase in organic waste that can reduce water quality. Phytoremediation is one way to stabilize pollutants, both organic and inorganic compounds, through aquatic plants. Water hyacinth (Eichornia crassipess) is one aquatic plant that can reduce total nitrogen up to 73.05%. This study aims to analyze the effect of water hyacinth (E. crassipess) as a phytoremediator on TAN reduction, survival rates, and growth of catfish (C. gariepinus) seeds. This study used a Completely Randomized Design (CRD) method with four treatments and four replications, namely P1 / control (without water hyacinth), P2 (without replacement of water hyacinth), P3 (alternation of water hyacinth every 21 days), and P4 (alternation of water hyacinth) every 14 days). The results showed that the P4 treatment (alternation of water hyacinth every 14 days) gave the lowest TAN content 0.01 mg / L and provided the highest survival rate of 89.88%, as well as the absolute weight and length growth of the most catfish seeds height, i.e., 9.80 g, and 5.50 cm, respectively.


2019 ◽  
Vol 8 (2) ◽  
pp. 249
Author(s):  
I Kadek Wirawan ◽  
Pande Ketut Diah Kencana ◽  
I Made Supartha Utama

Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan waktu pengeringan terhadap karakteristik kimia dan sensorik teh daun bambu Tabah. Penelitian ini dirancang menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor, yaitu faktor suhu pengeringan (50o C dan 60o C) dan faktor waktu pengeringan (1 jam, 2 jam, dan 3 jam). Perlakuan diulang tiga kali untuk mendapatkan 18 unit percobaan. Parameter yang diamati adalah kadar air, pH, total asam, total fenol, dan organoleptik. Hasil penelitian menunjukkan bahwa interaksi suhu dan lama pengeringan daun teh bambu Tabah memiliki pengaruh berbeda nyata terhadap kadar air, pH, total asam, total fenol, warna, penerimaan keseluruhan, tetapi tidak memiliki pengaruh yang berbeda nyata terhadap aroma dan rasa, dengan perlakuan terbaik ini, kadar air, pH, asam total, fenol total, warna, aroma, rasa dan penerimaan keseluruhan ditemukan kadar air 6,881%, pH 6,6, total asam 0, 73%, total fenol 84,01mg / 100g, warna 4,33, aroma 4,40, rasa 3,93, dan penerimaan keseluruhan 4,07.   This study aimd to determine the effect of temperature and drying time on the chemical and sensory characteristics of Tabah bamboo leaf tea. This study was designed using a Completely Randomized Design (CRD) with two factors, namely the drying temperature factor (50oC and 60oC) and the drying time factor (1 hour, 2 hours, and 3 hours). The treatment was repeated three times to obtain 18 experimental units. The parameters observed were water content, pH, total acid, total phenol, color, aroma, taste, and overall acceptance tested organoleptically. The results showed that the interaction of temperature and duration of drying of Tabah bamboo tea leaves had significantly different effects on water content, pH, total acid, total phenol, color, overall reception, but did not have a significantly different effect on aroma and taste. with this combained best treatment, the water content, pH, total acid, total phenol, color, aroma, taste and overall acceptance were found water content 6,881 %, pH 6,6, total acid 0, 73%, total phenol 84,01mg / 100g,color 4,33, aroma 4.40, taste 3,93, and overall acceptance 4,07.


2019 ◽  
Vol 7 (2) ◽  
pp. 823
Author(s):  
Nur Selawati ◽  
Indra Gumay Yudha ◽  
Deny Sapto Chondro Utomo

Hoven’s carp is one of the potential fish in Indonesia to be developed. But due to its slow growth, making people not cultivating it. This research aimed to study the effect of using rGH and determine the best dosage on growth of hoven’s carp. Hoven’s carp used were 70 - 90 mm in size and 5 - 6 g in weight 4 months old. Hoven’s carp used are 10 fish in each pond This study used a completely randomized design with 4 treatments and 3 replications. The treatment was A (without administration of rGH), B (administration of rGH 1 mg/kg of feed), C (administration of rGH 2 mg/kg of feed), and D (administration of rGH 3 mg/kg of feed). Parameters observed consisted of growth absolute length, the growth of absolute weight, SR, FCR, and protein retention. The results showed that the effect on hoven’s carp growth of rGH and best dose in this study is in treatment B (administration of rGH 2 mg/kg of feed)


2006 ◽  
Vol 8 (1) ◽  
pp. 25
Author(s):  
Des Roza ◽  
Fris Johnny ◽  
Tridjoko Tridjoko

An experiment to evaluate the effectiveness immunostimulant and bacterin on humpback grouper have been conducted at the Disease Laboratory of Research Institute for Mariculture, Gondol, Bali. The experiment was designed in completely randomized design with four treatments in duplicates. Two hundred of humpback grouper juveniles (15-18 cm of total length, 55-65 g of body weight) were injected intraperitoneally with (A) bacterine at 107 cfu/kg body weight (BW), (B) peptidoglycan at 100 mg/kg BW, and (C) immuno star at 100 ml/kg BW, (D) control. The fish were then challenged with VNN by intramuscular injection at 10 days post treatment. Results showed that survival rates of juvenile after challenged with VNN were 60.00% (B & C), 53.34% (A), and 1.67% (control). Parameters of non-spesific immune respons showed that phagocytic activities were 17.56% (B), 17.55% (C), 13.11% (A), and 9.33% (control). In addition, lisozyme activities were 1.64 cm (B), 1.58 cm (C), 1.55 cm (A), and 1.46 cm (control). Immunostimulant and bacterin stimulated non-specific immune response, and increased survival rate of humpback grouper juvenile.


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