MicroRNA-150 regulates steroidogenesis of mouse testicular Leydig cells by targeting STAR

Reproduction ◽  
2017 ◽  
Vol 154 (3) ◽  
pp. 229-236 ◽  
Author(s):  
Xu-Jing Geng ◽  
Dong-Mei Zhao ◽  
Gen-Hong Mao ◽  
Li Tan
Keyword(s):  
Leydig Cells ◽  
Reproductive Development ◽  
Negative Control Group ◽  
Negative Control ◽  
Sex Steroid ◽  
Luciferase Reporter ◽  
Control Group ◽  
Reporter Assay ◽  
Group A

Leydig cells are essential for male reproductive development throughout life. Production of androgens as well as intermediate steroids is tightly regulated. Although microRNAs (miRNAs) are suggested to play important roles in spermatogenesis, little is currently known regarding the regulation of steroidogenesis by miRNAs in Leydig cells. Here, we found that miR-150 was predominantly expressed in Leydig cells within mouse testis. Therefore, we determined steroidogenesis of the Leydig cells in which miR-150 was knocked down or overexpressed using miR-150 antagomir and agomir, respectively. Compared with negative control group, a significant increase of STAR expression was observed in miR-150 antagomir-treated Leydig cells. Conversely, STAR expression was significantly reduced in miR-150 agomir-transfected Leydig cells. Production of sex-steroid precursors and testosterone of Leydig cells was also negatively controlled by miR-150. We further identifiedStaras a target of miR-150 using luciferase reporter assay. Finally, we confirmed that miR-150 was necessary for steroidogenesis and spermatogenesisin vivovia intratesticular injection of miR-150 antagomir or agomir. Taken together, our studies suggest that miR-150 negatively regulates the expression of STAR and steroidogenesis of Leydig cells in mice.

scholarly journals Antiproliferation Effects of Nanophytosome-Loaded Phenolic Compounds From Fruit of Juniperus Polycarpos Against Breast Cancer in Mice Model: Synthesis, Characterization and Therapeutic Effects

2021 ◽  
Author(s):  
Soheila Moeini ◽  
Ehsan Karimi ◽  
Ehsan Oskoueian
Keyword(s):  
Breast Cancer ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Therapeutic Effects ◽  
Mice Model ◽  
Fruit Extract ◽  
Phenolic Fraction ◽  
Juniperus Polycarpos

Abstract Background: This research was performed to synthesize nanophytosomes-loaded high phenolic fraction (HPF) from Juniperus polycarpos fruit extract and investigate its antiproliferation effects against breast cancer in mice model. Results: The nanophytosomes-loaded HPF from Juniperus polycarpos fruit extract was synthesized. The mice trial was conducted to determine the possible toxic effects of the synthesized nanophytosomes. The anticancer, pro-apoptotic, and antioxidative activities of the nanophytosomes were determined. The nanophytosomes-loaded HPF had a spherical structure with a size of 176 nm and a polydispersity index coefficient of 0.24. The in-vivo study manifested that nanophytosomes-loaded HPF significantly improved weight gain and food intake compared to the negative control group (p<0.05). The nanophytosomes-loaded HPF significantly enhanced the expression of bax (3.4-fold) and caspase-3 (2.7-fold) genes but reduced bcl2 (3.6-fold) gene expression in tumor cells. The average tumor size was significantly decreased in mice treated with nanophytosomes-loaded HPF (p<0.05). The expression of GPX (2.3-fold) and SOD (2.7-fold) antioxidants in the liver of mice supplemented with nanophytosomes-loaded HPF was significantly developed compared to the negative control (p<0.05). The nanophytosomes-loaded HPF did not show toxicity on normal cells. Conclusion: Our results indicated that nanophytosomes-loaded HPF might be a potential anticancer agent for the breast cancer treatment.

scholarly journals THE PROTECTIVE ROLE OF OMEGA-3 AGAINST GENOTOXICITY AND REPRODUCTIVE TOXICITY OF COBALT OXIDE NANOPARTICLES ACUTE TREATMENT IN MALE MICE

2018 ◽  
Vol 11 (5) ◽  
pp. 423
Author(s):  
Nahed A Hussien ◽  
Hanan R. H. Mohamed
Keyword(s):  
Cobalt Oxide ◽  
Negative Control Group ◽  
Protective Role ◽  
Negative Control ◽  
Control Group ◽  
Omega 3 ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes

Objective: Cobalt nanoparticles (NPs), especially cobalt oxide NPs (Co3O4 NPs) are attracting unique shaped NPs that are used in different biomedical applications and medicine. Different in vitro studies report their toxic and carcinogenic effect but limited in vivo studies were present on its genotoxic potential. The present study was aimed to evaluate the genotoxic potential of Co3O4 NPs on bone marrow cells and sperms and the protective role of omega-3 in male albino mice.Methods: Animals were segregated into four groups that were orally treated for 3 consecutive days, Group 1: Negative control; Group 2: Omega-3 (250 mg/kg); Group 3: Co3O4 NPs (20 mg/kg); and Group 4: Combined group (250 mg/kg Omega-3 and Co3O4 NPs 20 mg/kg).Results: The present results show that Co3O4 NPs administration significantly increased number of micronucleated polychromatic erythrocytes (PCEs)/1000 PCEs, sperm abnormalities, and DNA damage, significantly decreased sperm motility and concentration in comparison to negative control group. However, Omega-3 administration in the combined group modulates the genotoxic potential of Co3O4 NPs in comparison to Co3O4 NPs group.Conclusion: The present study reports the genotoxic potential of Co3O4 NPs in vivo and assesses the protective role of Omega-3 administration due to its antioxidant effect.

scholarly journals Pengaruh Pemberian Cuka Apel 'A' terhadap Kadar MDA Hepar Tikus Wistar Jantan yang Diinduksi Parasetamol Dosis Toksik

2018 ◽  
Vol 6 (2) ◽  
pp. 272
Author(s):  
Niki Niki Rahmawati ◽  
Sugiyanta Sugiyanta ◽  
Elly Nurus Sakinah
Keyword(s):  
Treatment Group ◽  
Normal Control ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Toxic Dose ◽  
Group A ◽  
Post Hoc ◽  
Cytochrome P 450

  High dose of paracetamol is metabolized by cytochrome P-450 become free radical N-acetyl-p-benzoquinoneimine (NAPQI) but liver Glutathione (GSH) is not adequate to change it become nonreactive metabolite so that NAPQI bind to unsaturated fatty acid of cell membrane, causing lipid peroxidation and increase liver Malondialdehyde (MDA). 'A' apple vinegar contains anthocyanin with an antioxidant effect by electron donor to NAPQI and acetic acid to improve liver GSH level. The aim of research was to investigate the effect of 'A' apple vinegar on the rat liver MDA induced by toxic dose of paracetamol. Research groups consist of normal control (CMC Na 1% 1 ml for 14 days), negative control (CMC Na 1% 1ml for 14 days + paracetamol 291.6 mg/200gBW on the day 12nd,13rd,14th), and treatment group ('A' apple vinegar 0.4 ml/150gBW for 14 days + paracetamol 291.6 mg/200gBW on the day 12nd,13rd,14th). Liver MDA was measured on the day 15th with competitive ELISA. The average of normal control group was 21.58 ng/ml, negative control group was 70,71 ng/ml, treatment group was 37,67 ng/ml. One way ANOVA and Post hoc LSD test showed significantly differences between all groups (p<0,05). It can be concluded that 'A' apple vinegar had an effect on the liver MDA induced by toxic dose of paracetamol.   Keywords: Paracetamol, NAPQI, MDA, 'A' apple vinegar, antioxidant  

INFLUENCE OF JAMU MADURA “EMPOT SUPER” ON THE VAGINAL EPITHELIUM THICKNESS OF WHITE MICE (Rattus norvegicus) – AN IN VIVO STUDY

2017 ◽  
Vol 2 (1) ◽  
pp. 47
Author(s):  
Anik Listiyana
Keyword(s):  
Rattus Norvegicus ◽  
Negative Control Group ◽  
Negative Control ◽  
Vaginal Epithelium ◽  
In Vivo Study ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Epithelium Thickness

<p><em>The aim of this research is to determine the influence of jamu Madura “Empot Super” (JMES) on the vaginal epithelium thickness of Rattus norvegicus in vivo. This research is kind of “true experimental-post test only control group design”. The rats were given drinking JMES once daily PS (Per-Sonde) for a month, then the vagina was taken to be sample for HE colouring. The sample was observed by the binocular microscope (100 times magnification) to identify the changes in the thickness of their vaginal epithelium. Calculation of the vaginal epithelium thickness was counted on the 10 field of view chosen randomly by the blind method. The result show that the vaginal epithelium thickness increased with dose 0,17mg/BW, 0,34mg/BW, and 0,68mg/BW of JMES compared with negative control group. But, the vaginal epithelium thickness decrease at the dose 0,51mg/BW compared with negative control group.</em></p><p> </p><p><strong>Keywords</strong><strong>: </strong>Jamu Madura “Empot Super” (JMES), vaginal epithelium thickness, white mice (<em>Rattus norvegicus</em>), In Vivo study</p>

In vivo Antitumoral Effect of Plantago major L. Extract on Balb/C Mouse with Ehrlich Ascites Tumor

2007 ◽  
Vol 35 (05) ◽  
pp. 841-851 ◽  
Author(s):  
Mehmet Ozaslan ◽  
I. Didem Karagöz ◽  
M. Emin Kalender ◽  
I. Halil. Kilic ◽  
Ibrahim Sari ◽  
...  
Keyword(s):  
Weight Gain ◽  
Negative Control Group ◽  
Negative Control ◽  
Ehrlich Ascites Tumor ◽  
Control Group ◽  
Plantago Major ◽  
Ascites Tumor ◽  
Treatment Groups ◽  
Ehrlich Ascites

The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Ehrlich ascites tumor (EAT) bearing Balb/C mice in vivo. Thirty male Balb/C mice were divided into 5 groups: 3 treatment groups and 2 control groups (6 per group). Treatment groups and the negative control group were injected with EAT (1 × 106 cells) intraperitoneally to develop ascites tumor. P. major L. extract (1%, 2% and 3% concentration extracts, 0.1 ml/day/mouse) were given p.o. for 10 alternate days. The control group was treated with 0.9% NaCl solution (0.1 ml/day/mouse). The changes of body weight in animals were recorded. On the 11th day, all of the mice were sacrified and their tissues were stained with haematoxylen and eosin for pathological studies. Body weights of in 3 treatment groups and the negative control group were elevated because of tumor burden. The maximal weight gain was recorded in the negative control group and the minimal weight gain was recorded in Group I. Pathological studies showed that P. major L. extract (especially 1% concentration) has inhibitive effect on EAT. P. major has an inhibitory effect on EAT in a dose dependent manner.

scholarly journals Preparation and Evaluation of Azelaic Acid Topical Microemulsion Formulation: In Vitro and In Vivo Study

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 410
Author(s):  
Wan-Hsuan Hung ◽  
Ping-Kang Chen ◽  
Chih-Wun Fang ◽  
Ying-Chi Lin ◽  
Pao-Chu Wu
Keyword(s):  
Azelaic Acid ◽  
Skin Irritation ◽  
Topical Administration ◽  
Negative Control Group ◽  
Negative Control ◽  
Commercial Product ◽  
Control Group ◽  
Drug Delivery Carrier

The aim of this study was to design oil in water (O/W) microemulsion formulations for the topical administration of azelaic acid. The permeability of azelaic acid through rat skin and the anti-inflammatory activities of the formulations were conducted to examine the efficacy of the designed formulations. Skin irritation and stability tests were also performed. The permeability of azelaic acid was significantly increased by using O/W microemulsions as carriers. The edema index of ear swelling percentage was significantly recovered by the 5% drug-loaded formulation and a 20% commercial product, demonstrating that the experimental formulation possessed comparable effect with the commercial product on the improvement of inflammation. The experimental formulation did not cause significant skin irritation compared to the negative control group. Moreover, the drug-loaded formulation also showed thermodynamic stability and chemical stability after storage for 30 days. In conclusion, the O/W microemulsion was a potential drug delivery carrier for azelaic acid topical application.

scholarly journals Effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 1019-1027
Author(s):  
Lijie He ◽  
Jing Wang ◽  
Dandan Chang ◽  
Dandan Lv ◽  
Haina Li ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Luciferase Reporter ◽  
Control Group ◽  
Rt Pcr ◽  
Blank Control Group ◽  
Cervical Cancer Cells ◽  
Mrna And Protein Expression

AbstractObjectiveThis article aims to investigate the effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA.MethodsHeLa cells of cervical cancer were divided into five groups: blank control group, negative control group (miRNA-200b mimic NC), miRNA-200b mimic group, RhoA-negative control group, and RhoA overexpression group. Cells were collected 48 h after transfection. The expression levels of miRNA-200b were detected by RT-PCR. Target relationship between miRNA-200b and RhoA was verified by the dual-luciferase reporter assay. RhoA mRNA and protein expression were detected by western blot and RT-PCR methods. Flow cytometry was used to detect the apoptosis of cells in each group, and the CCK8 method was used to detect the proliferation of cells in each group. The mRNA and protein expression of Bax and cyclin D1 were detected by RT-PCR and western blot.ResultsThe results of the dual luciferase reporter assay showed that RhoA was the target gene of microRNA 200b. Compared with the blank control group and the miRNA-200b mimic-NC group, the proportion of apoptotic cells increased significantly in the miRNA-200b mimic group, and the proliferation of cells was inhibited (P < 0.05). After overexpression of RhoA, the percentage of apoptotic cells decreased and the ability of cell proliferation increased significantly (P < 0.05).ConclusionmiRNA-200b can inhibit the proliferation and promote the apoptosis of cervical cancer cells by targeting the RhoA gene.

scholarly journals POTENSI EKSTRAK JAHE MERAH (Zingiberaceae officinale rosc)SEBAGAI ANTIMIKROBA DAN PENINGKAT KUALITAS SPERMATOZOA MENCIT YANG DIINFEKSI BAKTERI Staphylococcus aureus SECARA INTRA URETHRA

WAHANA ◽  
2017 ◽  
Vol 69 (2) ◽  
pp. 1-7
Author(s):  
Ersanto Ersanto ◽  
Sukarjati Sukarjati
Keyword(s):  
Staphylococcus Aureus ◽  
Negative Control Group ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Ginger Extract ◽  
Laboratory Rats ◽  
Plate Count Method ◽  
Red Ginger

Red Ginger (Zingiberaceae officinale rosc) is known to be used as an anti-microbial andenhancing the quality of spermatozoa. This study aims to demonstrate of the red ginger extract(Zingiberaceae officinale rosc)potential as an antimicrobial and the quality enhancer of spermatozoain laboratory rats injected by Staphylococcus aureus to its urethra. The red ginger was extracted byethanol. The sample of this research was the spermatozoa of 30 mice that were injected byStaphylococcus aureus to its urethra. Potential test of red ginger extract on the laboratory ratsconducted by observing the spermatozoa’s motility, viability, morphology, the spermatozoa’sconcentration and the amount of spermatozoa leukocyte in the laboratory rats after the administrationof the red ginger extract for 35 days under the microscope. Antimicrobial activity test onStaphylococcus aureus was done by culturing the spermatozoa of laboratory rats (in vivo) afteradministering the red ginger extract for 35 days with total plate count method. The result of the studyshowed that there were differences between negative control group of laboratory rats and positivecontrol group of laboratory rats (laboratory rats injected with Staphylococcus aureus intra urethra)motility (p = 0.000), viability (p = 0.000), morphology (p = 0.000), concentration (p = 0.000), and theamount of leukocyte (p = 0.000). Whereas on the calculation of red ginger extract bacterial coloniesgive the significant effects p <0.05 on the growth of S. aureus. Based on the results of this study, it canbe conclude that the red ginger has potential as an antimicrobial and it also can improve the quality ofspermatozoa in laboratory rats infected with S. aureus through its urethra.

scholarly journals Gambaran histopatologik lambung tikus wistar yang diberikan ekstrak daun sirsak (Annona muricata L.) setelah induksi aspirin

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Chelyne F. Sundalangi ◽  
Lily Loho ◽  
Carla F. Kairupan
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Inflammatory Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
Treatment Groups ◽  
Group A ◽  
Gastric Mucous

Abstract: Aspirin is an anti-inflammatory drug which can cause side effect such as damage of the gastric mucous. Soursop leaf is usually used for medical treatment because it contains flavonoid compound which has the antioxidant and anti-inflamatory activity and may protect gastric mucous from the side effects of aspirin. This study aimed to reveal the histopathological features of the gaster of wistar rats administered with soursop leaf extract after induced with aspirin. This was an experimental study using 20 Wistar rats. Rats were divided into negative control group (A) and treatment groups. Treatment groups were divided into; rats induced with aspirin 30mg for 10 days (B); rats administered with soursop leaf extract 80mg before induced with aspirin 30mg for 10 days (C); rats induced with aspirin 30mg for 10 days and administered with soursop leaf extract 80mg for the next 3 days (D); and rats induced with aspirin 30mg for 10 days and not treated for the next 3 days (E). Groups A, B and C were terminated on 11th day, meanwhile groups D and E were terminated on 14th day. The results showed normal histological features in group A. Group B showed acute gastritic features such as many PMN inflammatory cells in the mucous to serous layers, submucous edema, and capillary dilatation. Groups C and D showed many PMN inflammatory cells in the mucous to submucous layers. Group E showed decreased PMN inflammatory cells in mucous to submucous layers. Conclusion: Administration of soursop leaf extract could not decrease the acute gastritic signs such as inflammatory cells, edema and capillary dilatation in the gaster of Wistar rats induced with aspirin.Keywords: aspirin, soursop leaves, gaster. Abstrak: Aspirin merupakan obat anti inflamasi yang bisa menyebabkan efek samping gangguan mukosa lambung. Daun sirsak sering digunakan sebagai obat tradisional yang berkhasiat karena mengandung senyawa flavonoid yang berkhasiat sebagai antioksidan dan antiinflamasi yang mungkin dapat melindungi lambung dari efek samping aspirin. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik lambung tikus Wistar yang diberikan ekstrak daun sirsak setelah induksi aspirin. Jenis penelitian ini ialah eksperimental yang menggunakan 20 ekor tikus Wistar. Hewan uji dibagi dalam kelompok kontrol negatif (A) dan kelompok perlakuan. Kelompok perlakuan dibagi atas kelompok tikus yang diberi aspirin 30mg selama 10 hari (B); tikus yang diberi ekstrak daun sirsak 80mg sebelum induksi aspirin 30mg selama 10 hari (C), tikus yang diberi aspirin 30mg selama 10 hari dan diberikan ekstrak daun sirsak 80mg selama 3 hari berikutnya (D); dan tikus yang diberi aspirin 30mg selama 10 hari dan tidak diberi perlakuan selama 3 hari berikutnya (E). Kelompok A, B dan C diterminasi pada hari ke-11, kelompok D dan E diterminasi pada hari ke-14. Hasil penelitian menunjukkan gambaran histologik lambung normal pada kelompok A. Kelompok B menunjukkan gambaran histopatologik gastritis akut yakni baanyak sel-sel radang PMN pada lapisan mukosa sampai serosa, edema submukosa, dan pelebaran pembuluh darah kapiler. Kelompok C dan D menunjukkan banyak sel-sel radang PMN pada lapisan mukosa sampai submukosa. Kelompok perlakuan E menunjukkan sel-sel radang PMN yang lebih sedikit pada lapisan mukosa sampai submukosa. Simpulan: Pemberian ekstak daun sirsak tidak dapat mengurangi tanda-tanda gastritis akut berupa sel-sel radang, edema, dan pelebaran pembuluh darah kapiler pada lambung tikus wistar yang diinduksi aspirin. Kata kunci: aspirin, daun sirsak, lambung

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