Optimization of Ultrasonic Assisted Extraction Process on Antioxidant Activity of Honje Fruit Extract (Etlingera elatior) Using Surface Response Method

2021 ◽  
Vol 7 (2) ◽  
pp. 118-128
Author(s):  
Artta Gracia Malau ◽  
Asri Widyasanti ◽  
Selly Harnesa Putri
Keyword(s):  
Antioxidant Activity ◽  
Total Yield ◽  
Extraction Process ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Surface Response Method ◽  
Response Method ◽  
Etlingera Elatior

Honje fruit (Etlingera elatior) contains bioactive compounds as antioxidants. An antioxidant can be obtained by extraction. This research aimed to determine the best combination of solvent amount and extraction time to produce the optimal yield and antioxidant activity. The extraction method was UAE (Ultrasound Assisted Extraction) using ethanol 96% solvent amount 125 mL to 250 mL and time level of 30 to 60 minutes. The RSM (Response Surface Methodology) in the Design Expert 11 application was used to arrange the extraction combination treatment, which resulted in 13 runs. Parameters analyzed were total yield, antioxidant activity, pH, specific gravity, and color. The results showed that total yield was revealed quadratic, Y1 = 19.05 – 1.76A + 0.32B – 0.002AB + 0.023A2 – 0.0005B2 and the antioxidant activity was revealed linear, Y2 = 408.147 – 6.424A + 0.326B. The optimum treatment was achieved in the amount of solvent 174.815 ml and extraction time of 60 minutes resulted in a total yield of 17.125% and antioxidant activity of 77.55 ppm that could be classified as strong.

scholarly journals Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Bambang Kunarto ◽  
Ely Yuniarti Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Extraction Process ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain  bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

scholarly journals Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 582
Author(s):  
Inês Mansinhos ◽  
Sandra Gonçalves ◽  
Raquel Rodríguez-Solana ◽  
José Luis Ordóñez-Díaz ◽  
José Manuel Moreno-Rojas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Industrial Applications ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Contents ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvents ◽  
Ultrasonic Assisted

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.

scholarly journals Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

2020 ◽  
Vol 10 (2) ◽  
pp. 560
Author(s):  
Gregorio Iván Peredo Pozos ◽  
Mario Alberto Ruiz-López ◽  
Juan Francisco Zamora Nátera ◽  
Carlos Álvarez Moya ◽  
Lucia Barrientos Ramírez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Human Lymphocytes ◽  
Dry Weight ◽  
Extraction Process ◽  
Hibiscus Sabdariffa ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

scholarly journals Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization, HPLC Analysis, and Evaluation of Antioxidant Activity

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 425 ◽  
Author(s):  
Mengyang Hou ◽  
Wenzhong Hu ◽  
Aosheng Wang ◽  
Zhilong Xiu ◽  
Yusheng Shi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Flavonoids ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Pteris Cretica ◽  
Nitric Oxide Radical ◽  
Ultrasonic Assisted ◽  
Ic50 Values

In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) from Pteris cretica L. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7-O-glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS+•), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe2+) chelating capacity, with IC50 values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity from P. cretica.

scholarly journals Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Caroline Pagnossim Boeira ◽  
Natiéli Piovesan ◽  
Marcela Bromberger Soquetta ◽  
Déborah Cristina Barcelos Flores ◽  
Bruna Nichelle Lucas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Extraction Method ◽  
Ultrasonic Method ◽  
Ultrasound Assisted Extraction ◽  
Ultrasound Method ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Conventional Extraction

ABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasound-assisted extraction at various temperatures (20°C, 40°C and 60°C). Total phenolics, total flavonoids, antimicrobial activity and antioxidant activity were quantified by the methods of DPPH, FRAP, and ORAC, respectively. The conventional extraction method and ultrasound method influenced the phenolic content at all the tested temperatures. Flavonoids were not influenced by extraction methods. The antioxidant activity (DPPH) was highest in the ultrasonic method at temperatures of 40°C and 60°C; however, in the case of the FRAP method the best results were for the conventional extraction method. The conventional and ultrasonic methods did not influence the IC50 at temperatures of 20°C and 40°C, but using ORAC the antioxidant activity was influenced by the methods at all temperatures. The extract obtained at 60°C by the ultrasound method had high antimicrobial action in relation to the strains of Salmonella sp., Escherichia coli, and Staphylococcus aureus. Extraction ultrasonic-assisted can be considered adequate to obtain extracts of marcela, which are rich in bioactive compounds with high antioxidant activity.

scholarly journals Optimization of Extraction Conditions of Bioactive Compounds by Ultrasonic-Assisted Extraction from Artichoke Wastes

2021 ◽  
Vol 68 (3) ◽  
pp. 658-666
Author(s):  
Izzet Turker ◽  
Hilal Isleroglu
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Extraction Processes ◽  
Ultrasonic Assisted Extraction

In this study, bioactive compounds were extracted by ultrasonic-assisted extraction and classical extraction processes using distilled water as solvent from artichoke leaves which are considered as agricultural wastes. Antioxidant capacity, total phenolic and total flavonoid content values of the obtained bioactive extracts were determined, and extraction yields and times were evaluated to compare the extraction processes. Also, the optimum extraction conditions of ultrasonic-assisted extraction (extraction time and ultrasonic power) which provide the highest extraction yield were determined using D-optimal design by ‘desirability’ function approach. According to the results, bioactive extracts having high antioxidant capacity were obtained at shorter times and higher extraction yields were achieved by ultrasonic-assisted extraction process than classical extraction. The highest extraction yield was estimated as 98.46% with an application of 20.05 minutes of extraction time and 65.02% of ultrasonic amplitude for the ultrasonic-assisted extraction process.

Optimization of Ultrasonic-Assisted Extraction for Pinocembrin from Flos populi Using Response Surface Methodology

2017 ◽  
Vol 13 (5) ◽  
Author(s):  
Zunlai Sheng ◽  
Biying Wang ◽  
Jiahong Zhao ◽  
Wenhui Yu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Surface Model ◽  
Extraction Temperature ◽  
Acoustic Intensity ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Assisted

Abstract In this study, the ultrasound-assisted extraction (UAE) was used for extraction of pinocembrin from Flos populi. Based on the results of the single-factor experiment, four independent parameters, including ethanol concentration (40–80 %), extraction temperature (50–70 °C), extraction time (25–45 min) and electrical acoustic intensity (40.8–81.5 W/m2) were further investigated using response surface methodology (RSM) coupled with Box-Behnken design. The experimental data were fitted to the quadratic response surface model using multiple regression analysis with high adjusted determination coefficient value (R 2) of 0.9697. The highest yield (134.2 ± 1.53 mg/g) of pinocembrin was obtained under the optimal conditions (ethanol concentration of 68 %, extraction temperature of 69 °C, extraction time of 42 min and electrical acoustic intensity of 66.81 W/cm2), which agreed to the predicted value of 132.9 mg/g. Moreover, the comparison between the UAE and reflux extraction also showed the suitability of UAE for pinocembrin from Flos populi.

A ULTRASOUND-ASSISTED EXTRACTION (UAE) OF PHYTOCHEMICALS WITH RESPONSE SURFACE METHODOLOGY(RSM) IN Curcuma xanthorrhiza

2020 ◽  
Vol 2 (2) ◽  
pp. 139-146
Author(s):  
Najiba Azemi ◽  
Norazah Basar ◽  
Susilawati Toemen
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Array Detector ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The Curcuma genus belongs to the Zingiberaceae family is commonly known for its usage as spice and medicine.it is widely used in pharmaceutical, food supplement, cosmetic industries lead to economic benefits. Recently, noticing to an eco-friendly extraction approach which provides the extraction efficiency is considered that in the extraction process, exploring the optimum condition of extraction parameters. This study is performed to improve the bioactive compounds extraction from C. xanthorrhiza by ultrasound-assisted extraction (UAE) through Response Surface Methodology (RSM). Three independent variables: extraction time (5-20 min), temperature (30-50 ° C) and liquid-solid ratio (6-10 mL / g) are applied using Box-Behnken (BBD) design. Quantification of curcumin and xanthorrhizol are carried out using reversed-phase high-performance liquid chromatography (RP HPLC) equiped by a photodiode array detector. Extraction time 20 minutes, temperature of 50°C, and LS ratio 8 mL/g are found as the optimum condition of extraction yield and quantification of xanthorrhizol. Curcumin 's optimum state, however, is observed at extraction time 12.50 minutes, temperature 30 ° C, and liquid-solid ratio of 10 mL / g. This study suggests that there are favorable potential using method of ultrasound-assisted extraction under specific parameters in the extraction process which is advantageous for advanced research.  

scholarly journals Extraction Process Optimization, Identification, and Profiling of Phenolic Antioxidant Compounds from the Fruits of Ficus auriculata

2020 ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Process ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Experimental Findings ◽  
Optimum Extraction

Abstract In this study, the extraction conditions to maximize the antioxidant activity and total phenolic content of Ficus auriculata were optimized using response surface methodology. For the purpose of extraction, the Ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P<0.0001) and total phenolic content (R2 = 0.961, P<0.0001), indicating a significant correlation between the experimental and expected value. The highest antioxidant activity (85.20 ± 0.96 %) for DPPH were achieved at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC-ESI-MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

IMPROVED YIELD OF β-CAROTENE FROM MICROALGAE SPIRULINA PLATENSIS USING ULTRASOUND ASSISTED EXTRACTION

2015 ◽  
Vol 77 (1) ◽  
Author(s):  
H. Hadiyanto ◽  
M. A. Marsya ◽  
P. Fatkhiyatul
Keyword(s):  
Maximum Yield ◽  
Ultrasound Irradiation ◽  
Extraction Process ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Fixed Temperature ◽  
Ultrasound Frequency ◽  
Assisted Extraction ◽  
The Rich ◽  
Β Carotene

Microalgae Spirulina has been considered as the rich nutrition microorganism including β-carotene. The β-carotene plays an important role in human’s body as an antioxidant. The extraction of this compound from microalgae by using conventional method resulted low yield and required longer extraction time. The objective of this research was to investigate the use of ultrasound irradiation to assist extraction of β-carotene in order to accelerate the extraction process and increase the yield. The experiment was conducted by varying extraction variables such as solvent volume (20, 40, 60 ml) and extraction time (5, 15, 25 minutes) on a fixed temperature of 40°C.  The result showed that the optimum condition was at ultrasound frequency of 40kHz, extraction time of 25 minutes and ratio of biomass to solvent 1:6, while the maximum yield was 1.38 mg/mL. 

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