scholarly journals Allelopathy of Invasive Species Dioscorea bulbifera L. and Its Effect on Seed Germination of Shorea selanica (Lam.) Blume

2021 ◽  
Vol 14 (1) ◽  
pp. 101-114
Author(s):  
Ade Ayu Oksari ◽  
Irvan Fadli Wanda ◽  
Gladys Ayu Paramita Kusumah Wardhani
Keyword(s):  
Phenolic Compounds ◽  
Seed Germination ◽  
Native Species ◽  
Germination Rate ◽  
Seed Viability ◽  
Screening Tests ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Randomized Design ◽  
Tuber Extract

AbstrakDioscorea bulbifera L. (Dioscoreaceae) merupakan salah satu tanaman invasif yang menciptakan masalah lingkungan. D bulbifera mengandung alelopati yang memengaruhi proses fisiologis pada spesies lain. Penelitian ini bertujuan untuk mengetahui pengaruh alelopati berbagai konsentrasi ekstrak daun dan umbi D. bulbifera L. terhadap perkecambahan biji Shorea selanica (Lam.) Blume. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan sepuluh perlakuan dan tiga ulangan. Perlakuan yang diberikan adalah perbedaan konsentrasi ekstrak D. bulbifera 25, 50, 75, 100%, dan kontrol. Berdasarkan hasil uji skrining fitokimia, semua metabolit sekunder dalam ekstrak daun menunjukkan hasil yang positif. Senyawa golongan saponin, tanin, flavonoid, dan steroid/terpenoid terdeteksi dengan kuat sedangkan senyawa alkaloid kurang kuat. Senyawa fenol tersebut adalah fenol, 1,2-benzenediol (dalam ekstrak daun) dan fenol, 1,2-benzendiol, 1,4-benzendiol, dan 2-metoksifenol (dalam ekstrak umbi). Berdasarkan uji perkecambahan, ekstrak metanol umbi dan daun D. bulbifera berpengaruh nyata terhadap daya kecambah, koefisien kecepatan berkecambah, dan koefisien keserempakan berkecambah S. selanica. Konsentrasi yang menurunkan viabilitas biji terendah adalah pada konsentrasi 75% dan 100% dengan rata-rata daya kecambah 10,00 ± 6,32 % (pada ektrak umbi) dan 0,00 ± 6,32 % (pada ektrak daun). AbstractDioscorea bulbifera L. (Dioscoreaceae) is one of the most unutilized invasive plants. This plant contains allelopathy that affects the physiological process of native species. This study aimed to discover the type of allelochemical in D. bulbifera and its effect on seed germination of Shorea selanica (Lam.) Blume. The method used was a Completely Randomized Design with ten treatments and three replications. The treatment given was the different concentration of D. bulbifera extract of 25, 50, 75, 100%, and control. Based on phytochemical screening tests, saponin, tannin, flavonoid, and steroid/terpenoid compounds were detected in leaf and tuber extract. The total phenolic in leaf extract was more significant than that in the tuber extract of D. bulbifera but the type of phenolic compounds was lower. The phenolic compounds were phenol, 1,2-benzenediol (in leaf extracts) and phenol, 1,2-benzendiol, 1,4-benzendiol, and 2-methoxyphenol (in tuber extracts). Based on the germination test, it was found that the methanol extract from the tubers and leaf of D. bulbifera significantly affected the germination capacity, coefficient rate of germination, and simultaneity coefficient of germination of S. selanica. It was found that treatment of concentration of 75% and 100% resulted in the lowest seed viability reduction with an average germination rate of 10.00 ± 6.32% (in tuber extracts) and 0.00 ± 6.32% (in leaf extracts).

scholarly journals Allelopathic effect of invasive species air potato (Dioscorea bulbifera) on seeds germination of Polyalthia littoralis

2019 ◽  
Vol 11 (1) ◽  
pp. 63-70
Author(s):  
ADE AYU OKSARI ◽  
DEVY SUSANTY ◽  
IRVAN FADLI WANDA
Keyword(s):  
Invasive Species ◽  
Germination Rate ◽  
Seed Viability ◽  
Total Phenolic ◽  
Allelopathic Effect ◽  
Randomized Design ◽  
Dioscorea Bulbifera ◽  
Coefficient Of Velocity ◽  
Tuber Extract ◽  
Seeds Germination

Abstract. Oksari AA, Susanty D, Wanda IF. 2019. Allelopathic effect of invasive species air potato (Dioscorea bulbifera) on seeds germination of Polyalthia littoralis. Nusantara Bioscience 11: 63-70. Dioscorea bulbifera L. (Dioscoreaceae) is included in the Global Weed Compendium and one of the invasive plants that cause environmental problems in many parts of the world. This study aims to determine the effect of allelopathy from various concentrations of leaf and tuber extract from D. bulbifera on seed germination of Polyalthia littoralis (Blume) Boerl. The method used was a Completely Randomized Design (CRD) with seven treatments and three replications. The treatment given was the concentration of D. bulbifera extract 10%, 20%, 40%, 60%, 80%, 100% and aquadest solvent control. The results of phytochemical screening analysis showed that the extract of leaves and tuber of D. bulbifera did not contain alkaloids, but specifically on leaves extract contained tannins. Both extracts contained steroids, flavonoids, and saponins. Based on the results of the study, total phenolic obtained in the leaf extract as much as 28.81 ± 2.51 mg TAE/g extract, while the tuber extract had a total phenolic of 6.74 ± 1.19 mg TAE/g. The results showed that allelopathic substances of D. bulbifera affected the germination of P. littoralis seeds. Based on DMRT test at 5% level, it was known that allelopathy concentration of D. bulbifera significantly affected the germination rate at concentrations of 20%, 60%, 80%, and 100% and significantly affected the coefficient of velocity of germination at a concentration of 20%. But, concentration allelopathy of D. bulbifera had no significant effect on the coefficient of uniformity of germination. The concentration that reduced the lowest seed viability was at a concentration of 80% with an average germination power of 56.67 ± 7.45%.

scholarly journals Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
ISKANDAR Z. SIREGAR ◽  
KARIMA FAUZIAH MUHARAM ◽  
Y. ARIS PURWANTO ◽  
DEDE J. SUDRAJAT
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Physiological Activity ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Priming ◽  
Germination Capacity ◽  
Gmelina Arborea ◽  
Randomized Design ◽  
Germination Characteristics

Abstract. Siregar IZ, Muharam KF, Purwanto YA, Sudrajat DJ. 2020. Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming. Biodiversitas 21: 4558-4564.  Gmelina (Gmelina arborea) seed collected from tropical Indonesian forest experience seed deterioration during storage which is relatively faster than the gmelina seeds originating from temperate regions, such as India and Myanmar. Various treatments have been made to improve the seed viability and vigor after storage through various invigoration techniques. However, the utilization of promising novel technology such as ultrafine bubbles (UFB) has not been evaluated yet. The objective of this study was to determine the effect of seed priming using UFB, polyethylene glycol (PEG), and gibberellic acid (GA3) on the viability and vigor of gmelina seeds that have been stored for one and two years. This study was conducted by employing Completely Randomized Design (CRD) using three replicates. The study showed priming treatment had significant effect and formed average germination capacity of 80% for the seed stored for 1 year.  On the other hand, the priming treatment using PEG-0.8 MPa was the best treatment for germination capacity of 74.67% on 2 years stored seeds. UFB had significant effect on germination capacity, germination rate, growth uniformity, and germination value. This result was expected due to reactive oxygen species (ROS) produced by micro-nano bubbles that could increase physiological activity of the seed cells.

scholarly journals Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Viability ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Viability Test ◽  
Physiological Dormancy ◽  
Different Temperatures ◽  
Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

scholarly journals PENGGUNAAN Trichoderma Koningii PADA PERKECAMBAHAN SIRSAK (Annona muricata linn)

2019 ◽  
Vol 15 (3) ◽  
pp. 563
Author(s):  
Erida Derita Dalame ◽  
Bertje Richard Albert Sumayku ◽  
Jeany Polii - Mandang
Keyword(s):  
Seed Germination ◽  
Plant Protection ◽  
Germination Rate ◽  
Soaking Time ◽  
The North ◽  
North Sulawesi ◽  
Trichoderma Koningii ◽  
Randomized Design ◽  
Vigor Index ◽  
Two Factors

This study aims to determine the dose of Trichoderma koningii which can induce soursop germination; know the best soaking time that can affect the induction of soursop germination; to know the interaction between soaking time and Trichoderma koningii dose on soursop germination. This research was conducted in April - June 2019 at the Green House of Plant Sciences, Faculty of Agriculture, Sam Ratulangi University, Manado. The material used in this study was soursop seed obtained from Lota Village, Pineleng Tomohon Subdistrict, Trichoderma koningii with 106 solids obtained from the North Sulawesi Province Plant Protection Center Collection, Aquadest, 70% Alcohol, 80% Acetone, sandalready sifter and sterilized. This research was arranged in factorial with a Completely Randomized Design (CRD) consisting of two factors, namely 5 treatments of Trichoderma koningii dose and 4 immersion treatments repeated three times (3x) so that a total of 60 treatment units. Each treatment unituses 10 seeds. The results of this study indicated that the dose of Trichoderma koninggi 200 g without soaking can increase the germination power by 86.67% and the vigor index by 32.96%; Immersion with Koninggi Trichoderma suspension has not been able to induce soursop seed germination rate; Theinteraction between Trichoderma koningii dose and soaking time did not affect the speed of soursop seed germination.*eprm*

Age-related biochemical changes during storage of beech (Fagus sylvatica L.) seeds

2007 ◽  
Vol 17 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Stanislawa Pukacka ◽  
Ewelina Ratajczak
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Fagus Sylvatica ◽  
Total Sterol ◽  
Seed Viability ◽  
Soluble Proteins ◽  
Total Phenolic ◽  
Germination Capacity ◽  
Strong Negative Correlation ◽  
Age Related

AbstractSubstances that could play a role in maintaining seed viability (phenolic compounds, α-tocopherol, sterols, ascorbic acid, glutathione and soluble proteins) were estimated in beech (Fagus sylvatica L.) seed lots that had been stored for 2, 5, 7 and 10 years at − 10°C. Germination capacity was strongly and positively correlated with amounts of total phenolic compounds, ultraviolet (UV)-absorbing phenols and soluble proteins. Moderately strong relationships appeared between germination capacity and α-tocopherol and ascorbic acid contents. Total sterol and glutathione contents were not correlated with germination capacity. A strong, negative correlation was found between germination capacity and reactive oxygen species (ROS), such as superoxide radical (O _{2}^{\cdot - }) and hydrogen peroxide (H2O2), as well as with lipid hydroxyperoxides (LHPOs). The putative role of these compounds in the maintenance of beech seed viability during long-term storage is discussed.

scholarly journals Application of Young Coconut Water and “Kascing” Fertilizer to Red Palm Seed Germination

2017 ◽  
Vol 18 (1) ◽  
pp. 17
Author(s):  
Fatimatuz Zuhro ◽  
Hasni Ummul Hasanah ◽  
Sukadi Sukadi
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Water Concentration ◽  
Germination Percentage ◽  
Coconut Water ◽  
Randomized Design ◽  
Completely Randomized Design

Redpalm isornamentplantwhichpotentiallytobecultured.Thescarcityofredpalmmadeithashighmarket value and need to be preserved. However, the germination growth of red palm is slower than other kinds of palm, so the best way to germinate the red palm seed is needed. The aim of this research is to determine the effect of apliccation some young coconut water concentration and kascing fertilizer to red palm seed germination. The research used factorial completely randomized design with 5 replication, the first factor is young coconut water concentration (0%, 25%, 50%, 75%, and 100%) and the second factor is germination media (steril sand and kascing fertilizer). Variables measured were live germination percentage, speed germination rate, radicula length, and plumula height. Results of this study showed that the best germination media is steril sand and 0% concentration of young coconut water.

scholarly journals SETTINGS OF TEMPERATURE AND TIME SAVING ON SEED GERMINATION OF Magnolia champaca (L.) Baill. ex Pierre

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Odetta Maudy Nuradinda ◽  
Sri Pertiwi Estuningsih ◽  
Harmida Harmida
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Temperature Treatment ◽  
Seed Vigor ◽  
Control Treatment ◽  
Time Saving ◽  
Randomized Design ◽  
Vigor Index ◽  
Germination Vigor ◽  
Physiology Laboratory

Setting storage space temperature and time saving becomes an important application in supporting the development of plantations to provide seeds as needed and sustainable. The purpose of this research is to delay the rapid of seed germination in Magnolia champaca by assessing the effect of various suspend temperatures and times saving on seed germination of Magnolia camphaca . The research was conducted in Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University in February until June 2016. The method was used completely randomized design pattern Factorial by 3 factors with 12 treatments and 3 replications with the temperature: temperature ± 31°C, temperature ± 25 °C, temperature ± 18 °C, temperature ± 4 °C and the retention of time 0 week, 2 weeks , 4 weeks and 6 weeks. The parameters include the observation of germination rate, germination, vigor index and sprout morphology. The results of the research the treatment of interaction of temperature and time saving significantly affected on germination rate, germination and seed vigor index of Magnolia champaca. Germination speed, germination and vigor index were both produced by the treatment of storage at a temperature of ± 18 °C and a temperature of ± 25 °C at all times to keep (2 weeks, 4 weeks, 6 weeks) compared to control treatment. A normal sprout produced in temperature treatment ± 18 °C and ± 25 °C temperature ranges between 90-93%. An abnormal sprout produced in control treatment (0 week) by 25%. Measurement of moisture content and physiological maturity level of Magnolia champaca seed needs to be done before it is stored and germinated to test the viability of seeds of sprouts.

scholarly journals ​Influence of Temperature and Salinity Stress on Seed Germination and Seedling Growth of Soybean (Glycine max L.)

2021 ◽  
Author(s):  
Mustafa Çirka ◽  
Ali Rahmi Kaya ◽  
Tamer Eryiğit
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Rate ◽  
Petri Dish ◽  
Negative Effects ◽  
Randomized Design ◽  
Salt Level ◽  
Glycine Max L ◽  
Early Seedling ◽  
Lower Temperature

Background: The high salt level of a germinating environment can lead to reduced, delayed, and even complete inhibition of germination and seeding growth due to osmotic action and/or ion toxicity. Based on this viewpoint, the aim of this study was to investigate germination temperature and salinity effects on germination and early seedling growth of soybean, which can be sown as first and second crops. Methods: Soybean seeds were subjected to NaCl induced saline germinating media prepared in petri dishes under two different germination temperatures (20±2 and 25±2oC). Thirty sterilized seeds per petri dish were sown in ten salt treatments (0, 100, 150, 250, 750, 1000, 2250, 5000, 7500 and 10000 ppm NaCl L-1). The study was carried out according to the completely randomized design with four replications. Result: Lower temperature promoted seed germination, while the high temperature significantly inhibited the seed germination at all NaCl doses tested. As a result, 25oC temperatures, which can only be measured at the time of the second sowing, have been found to negatively affect germination and also increase the negative effects of salt. Due to the moderate tolerance of soybeans to salt stress, the germination rate was positively affected up to 750 ppm NaCl L-1 dose and resulted in severe reductions in subsequent doses. Also, the tolerance of soybean was negatively influenced by the interaction of temperature and NaCl concentration.

The combined treatments of Canada goldenrod leaf extracts and cadmium pollution confer an inhibitory effect on seed germination and seedling development of lettuce

2018 ◽  
Vol 66 (4) ◽  
pp. 331 ◽  
Author(s):  
Congyan Wang ◽  
Kun Jiang ◽  
Bingde Wu ◽  
Jiawei Zhou
Keyword(s):  
Heavy Metals ◽  
Invasive Species ◽  
Seed Germination ◽  
Native Species ◽  
Inhibitory Effect ◽  
Seedling Development ◽  
Combined Treatments ◽  
High Concentration ◽  
Leaf Extracts ◽  
Allelopathic Effects

Increasing levels of heavy metals are released into ecosystems. The influence of heavy metal pollution on successful invasive species has raised considerable interest, particularly regarding potential allelopathic effects on seed germination and seedling development of native species. Adding heavy metals may alter or even enhance such allelopathic effects of invasive species. The aim of the present study was to address the combined treatments of the invasive species Canada goldenrod (Solidago canadensis L.) leaf extracts and cadmium (Cd) pollution on seed germination and seedling development of the homologous native species lettuce (Lactuca sativa L.). Results showed that the combined treatments of Canada goldenrod leaf extracts and Cd pollution significantly decreased seed germination and seedling development of lettuce compared with the control. In addition, the indices of allelopathic effects for all seed germination and seedling development indices of lettuce were less than zero under the combined treatments of high concentration of Canada goldenrod leaf extracts and Cd pollution (regardless of concentration). Thus, the combined treatments of high concentration of Canada goldenrod leaf extracts and Cd pollution pose an inhibitory effect on seed germination and seedling development of lettuce. Further, the combined treatments of high concentration of Canada goldenrod leaf extracts and Cd pollution (regardless of concentration) cause more toxic effects than the combined treatments of low concentration of Canada goldenrod leaf extracts and Cd pollution (regardless of concentration) on all seed germination and seedling development indices of lettuce. Thus, the inhibitory effects of Canada goldenrod on the seed germination and seedling development of co-occurring native species may be intensified at heavy invasion degrees under increased heavy metal pollution. Subsequently, the competitiveness and fitness of native species may be notably decreased via the reduced seed germination and seedling development and then the notorious invader can establish progressively-growing populations in the colonised ecosystems.

scholarly journals Study of Seed Germination by Soaking Methode of Cacao (Theobroma cacao L.)

2014 ◽  
Vol 30 (3) ◽  
pp. 190-197
Author(s):  
Sulistyani Pancaningtyas ◽  
Teguh Iman Santoso ◽  
Sudarsianto .
Keyword(s):  
Seed Germination ◽  
Theobroma Cacao ◽  
Germination Rate ◽  
Seed Viability ◽  
Random Design ◽  
Experimental Station ◽  
Low Costs ◽  
Theobroma Cacao L ◽  
Cocoa Seedlings

Study of germination methods conduct to get information about seed viability based on germination rate, percentage of germination and vigority. Germination methods was studied to get the efficiency and effectivity of germination, easy to handle, low costs with high vigority. Sand and gunny sack methods  for germination, need extensive place  and 3-4 days germination period after planting. This research will study the alternative of germination method with soaking. This method can be accelerating  germination rate and effectively place usage without decreasing the quality of cacao seedling.The research was done at Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institue. This research consist of two experiment was arranged based on factorial completely random design. First experiment will observed to compared germination rate and the second experiment will observed seedling quality between soaking and wet gunny sack germination method.The results showed that length of radicel on soaking method longer than wet gunny sack method. Growth of radicel started from 2 hours after soaking, moreover length of radicel at 4 hours after soaking have significant different value with gunny sack method. On 24 hours after soaking have 3,69 mm and 0,681 mm on wet gunny sack treatment. Except lengt of hipocotyl, there is not different condition between seedling that out came  from soaking and wet gunny sack method. Length of hipocotyl on 36 hours after soaking have 9,15 cm and significant different between wet gunny sack germination method that have 5,40 cm. Keywords : seed germination, soaking method, Theobroma cacao L., cocoa seedlings

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