Activity Test of Lemon Essential Oil (Citrus limon Burm.) Shampoo Gel as Antidandruff against Fungus Malassezia sp.

Background: Neuropathic pain is one of the contributors to the global burdens of illness. At present many patients do not achieve satisfactory pain relief even with synthetic pain-killers. Taking this into consideration, it is necessary to search for natural product-derived alternative treatment with confirmed safety and efficacy. Ageratum conyzoides L is a plant often used as analgesic in Indonesia, however, anti-neuropathic pain activity of this plant is still unknown. Objective: To determine the anti-neuropathic pain activity of the essential oil and non-essential oil component (distillation residue) of A. conyzoides L. Methods: We conducted separation of the essential oil component from other secondary metabolites through steam distillation. Both components were tested for anti-neuropathic pain activity using chronic constriction injury animal models with thermal hyperalgesia and allodynia tests. The animals were divided into 7 test groups namely normal, sham, negative, positive (pregabalin at 0.195 mg/20 g BW of mice), essential oil component (100 mg/kg BW), and non-essential oil component (100 mg/kg BW). Naloxone was tested against the most potent anti-neuropathic pain component (essential oil or nonessential oil) to investigate the involvement of opioid receptor. Results: The GC-MS of the essential oil component indicated the presence of 60 compounds. Meanwhile, non-essential oil components contained alkaloid, flavonoid, polyphenol, quinone, steroid, and triterpenoid. This non-essential oil component contained a total flavonoid equivalent to 248.89 ppm quercetin. The anti-neuropathic pain activity test showed significantly higher activity of the essential oil component compared to the non-essential oil component and negative groups (p<0.05). Furthermore, the essential oil component showed equal activity to pregabalin (p>0.05). However, this activity was abolished by naloxone, indicating the involvement of opioid receptor in the action of the essential oil component. Conclusion: The essential oil component of A. conyzoides L is a potential novel substance for use as anti-neuropathic pain.

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Taking essential oil by water distillation and antibacterial activity test of refined water from agarwood plant parts (Aquilaria malaccensis Lamk)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/782/3/032076 ◽

2021 ◽

Vol 782 (3) ◽

pp. 032076

Author(s):

M Nurminah ◽

R Batubara ◽

T Ismanelly ◽

Albert

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Water Distillation ◽

Plant Parts ◽

Activity Test ◽

Aquilaria Malaccensis

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Evaluation of Citrus limon peels essential oil on growth performance, immune response of Mozambique tilapia Oreochromis mossambicus challenged with Edwardsiella tarda

Aquaculture ◽

10.1016/j.aquaculture.2016.08.023 ◽

2016 ◽

Vol 465 ◽

pp. 13-18 ◽

Cited By ~ 47

Author(s):

Esin Baba ◽

Ümit Acar ◽

Canan Öntaş ◽

Osman Sabri Kesbiç ◽

Sevdan Yılmaz

Keyword(s):

Immune Response ◽

Essential Oil ◽

Growth Performance ◽

Oreochromis Mossambicus ◽

Edwardsiella Tarda ◽

Citrus Limon ◽

Mozambique Tilapia

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Protective effect of essential oil from Citrus limon against aspirin-induced toxicity in rats

Human & Experimental Toxicology ◽

10.1177/0960327118819044 ◽

2018 ◽

Vol 38 (5) ◽

pp. 499-509 ◽

Cited By ~ 4

Author(s):

H Bouzenna ◽

N Samout ◽

S Dhibi ◽

S Mbarki ◽

S Akermi ◽

...

Keyword(s):

Essential Oil ◽

Protective Effect ◽

Antioxidant Activities ◽

Reducing Power ◽

Female Rats ◽

Control Group ◽

Albino Rats ◽

Citrus Limon ◽

Β Carotene ◽

Aspirin Group

The present study is planned to examine the antioxidant activity (AA) and the protective effect of the essential oil of Citrus limon (EOC) against aspirin-induced histopathological changes in the brain, lung, and intestine of female rats. For this purpose, 28 albino rats were classified to control group (group C), aspirin group (group A), EOC group (group EOC), and pretreatment with EOC and treated with aspirin group (group EOC + A). The antioxidant activities of EOC were evaluated by three different assays including reducing power, β-carotene, and scavenging of hydrogen peroxide (H2O2). Our results found that EOC represents, respectively (0.064 ± 0.013 and 0.027 ± 00 mg Quer E/100 µL), of flavonoid and flavonol. Then, it exhibited a potential activity of reducing power (at 300 mg/mL, which was found to be 0.82 ± 0.07), β-carotene-linoleic acid (AA% = 69.28 ± 3.5%), and scavenging of H2O2 (IC50 = 0.23 ± 0.008 mg/mL). In vivo, aspirin given to rats at the dose of 600 mg/kg body weight induced histomorphological damage in brain, lung, and intestine. However, our data found that the pretreatment with EOC offered a significant protection against the injury induced by aspirin. It can be concluded that the protective effect of EOC can be due to its antioxidant activities.

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Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i2.5560 ◽

2019 ◽

Vol 6 (2) ◽

pp. 181

Author(s):

Laila Nur Rohma ◽

Osfar Sjofjan ◽

M. Halim Natsir

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Feed Additives ◽

Diffusion Method ◽

Disc Diffusion ◽

Activity Test ◽

Main Component

ABSTRAK Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

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Lemon (Citrus limon, Burm.f.) essential oil enhances the trans-epidermal release of lipid- (A, E) and water- (B6, C) soluble vitamins from topical emulsions in reconstructed human epidermis

International Journal of Cosmetic Science ◽

10.1111/j.1468-2494.2012.00725.x ◽

2012 ◽

Vol 34 (4) ◽

pp. 347-356 ◽

Cited By ~ 13

Author(s):

L. Valgimigli ◽

S. Gabbanini ◽

E. Berlini ◽

E. Lucchi ◽

C. Beltramini ◽

...

Keyword(s):

Essential Oil ◽

Lipid A ◽

Human Epidermis ◽

Citrus Limon ◽

Reconstructed Human Epidermis

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Biological Properties of a Citral-Enriched Fraction of Citrus limon Essential Oil

Foods ◽

10.3390/foods9091290 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1290

Author(s):

Marzia Pucci ◽

Stefania Raimondo ◽

Chiara Zichittella ◽

Vincenza Tinnirello ◽

Valeria Corleone ◽

...

Keyword(s):

Oxidative Stress ◽

Essential Oil ◽

Biological Activities ◽

Protective Action ◽

Biological Properties ◽

Citrus Limon ◽

Tnf Α ◽

Pre Treatment ◽

Flavoring Agent ◽

And Oxidative Stress

Lemon essential oil (LEO) is a well-known flavoring agent with versatile biological activities. In the present study, we have isolated and characterized four citral-enriched fractions of winter LEO. We reported that in murine and human macrophages the pre-treatment with a mix of these fractions (Cfr-LEO) reduces the expression of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 induced by LPS. In addition, Cfr-LEO counteracts LPS-induced oxidative stress, as shown by the increase in the GSH/GSSG ratio in comparison to cells treated with LPS alone. Overall, the results reported here encourage the application of EO fractions, enriched in citral, in the nutraceutical industry, not only for its organoleptic properties but also for its protective action against inflammation and oxidative stress.

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