scholarly journals Морфолого-культуральні властивості рідкісного лікарського гриба Sparassis crispa (Sparassidaceae, Polyporales)

2017 ◽  
Vol 7 (4) ◽  
pp. 550-558
Author(s):  
O. B. Mykchaylova ◽  
A. P. Gryganskyi ◽  
M. L. Lomberg ◽  
N. A. Bisko
Keyword(s):  
Growth Rate ◽  
Growth Parameters ◽  
Molecular Genetic ◽  
Dna Typing ◽  
Morphological Characteristics ◽  
Secretory Cells ◽  
Nutrient Media ◽  
Mycelium Growth ◽  
Pine Sawdust

<p>A verification of <em>S</em><em>parassis</em><em> crispa</em><em> </em>(Wulfen) Fr. strains of the IBK Mushroom Culture Collection using molecular-genetic and cultural-morphological methods were done. For all <em>S. crispa</em> strains a complete determination of the nucleotide sequences of the internal transcribed spacer: ITS1, 5.8S and ITS2 regions of rRNA, as well as the partial determination of 18S and 28S sequences surrounding the ITS, was performed. As a result of the search in the gene bank, the <em>S</em><em>. </em><em>crispa</em> samples deposited there showed 99-98% identity with the sequences we received, thus confirming the species of the examined strains. On the basis of the sequences we received, investigated strains were registered in NCBI GenBank. To confirm the taxonomic affiliation of the strains we studied their cultural and morphological characteristics. Microstructures of vegetative mycelium by optical and scanning electron microscopy were investigated. In all <em>S. crispa</em> cultures we observed the hyphae with regular one-sided gapless clamp connections, numerous secretory cells on the surface of the hyphae, anastomoses, filamentous strands and films. We studied the growth rate and morphology of the strains on seven agar nutrient media. According to the radial growth rate of <em>S. crispa</em> cultures can be placed to the very slowly growing mushrooms, growth rate of 0,5−2,8 mm/day. We found the selective media such as malt agar with the addition of pine sawdust (SS) and larch sawdust (SM) as most favorable for the vegetative growth and generative stage for all strains, temperature of incubation was 26 ± 0.1 °C. For mycelial growth the critical temperature was 39 ± 0.1 °C for <em>S. crispa</em> strains 312, 314 and 40 ± 0.1 °C for the strains 304, 2004. We confirmed belonging of studied strains to <em>S. </em><em>с</em><em>rispa</em> species according to established morphological and cultural characteristics. This result coincided with the results of DNA typing. Thus, the obtained mycelium growth parameters on nutrient media, micro- and macromorphological characteristics can be used as additional taxonomic characteristics of <em>S. </em><em>с</em><em>rispa</em> culture in the vegetative stage of growth.<strong> </strong>The strain <em>S. </em><em>с</em><em>rispa</em> 314 may become a potential producer for new fungal biotechnologies in Ukraine in the near future and reintroduction of this species in the nature.</p>

Determination of growth parameters for atomic layer epitaxy using reflectance difference spectroscopy

1996 ◽  
Vol 74 (S1) ◽  
pp. 85-88 ◽  
Author(s):  
R. Arès ◽  
C. A. Tran ◽  
S. P. Watkins
Keyword(s):  
Growth Rate ◽  
Growth Parameters ◽  
Atomic Layer ◽  
Atomic Layer Epitaxy ◽  
Gas Flows ◽  
Difference Spectroscopy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Reflectance Difference Spectroscopy

Reflectance difference spectroscopy (RDS) has been used to monitor the anisotropy of the surface of InAs and GaAs grown by atomic layer epitaxy (ALE). Saturation of the RDS signal is observed when the surface is fully covered with one monolayer of the impinging surface species. This property is used to optimize the growth interruptions for the ALE cycle. Good correlation of the RDS saturation is observed with growth-rate measurements obtained by X-ray diffraction (XRD). When exposure times are sufficiently long for saturation to be observed in the RDS signal, a growth rate of one monolayer per cycle (1 ML/cycle) is achieved. In principle all the different growth parameters such as exposure and purge times as well as gas flows can be determined in a few cycles performed on a single substrate. Without RDS the same results would require several growth runs and time consuming X-ray characterization.

scholarly journals Cultivation of the diatom algae Chaetoceros calcitrans f. pumilus (Paulsen) Takano, 1968 as food for giant oyster larvae Crassostrea gigas (Thunberg)

2019 ◽  
Vol 4 (2) ◽  
pp. 34-40 ◽  
Author(s):  
L. V. Ladygina ◽  
A. V. Pirkova
Keyword(s):  
Growth Rate ◽  
Crassostrea Gigas ◽  
Nutrient Medium ◽  
Growth Parameters ◽  
Inorganic Nitrogen ◽  
Diatom Algae ◽  
Nitrogen And Phosphorus ◽  
Nutrient Media ◽  
Chaetoceros Calcitrans ◽  
Giant Oyster

An impact of modified nutrient media F/2 and Conway on the growth and biomass accumulation of the diatom algae Chaetoceros calcitrans f. pumilus, which is a part of the food for cultivated larvae of the giant oyster Crassostrea gigas in the IMBR RAS nursery, was studied. Maximum values of cell and biomass concentrations were obtained on the modified F/2 nutrient medium (11.22 × 106 cells·ml-1 and 4.93 g·l-1, respectively), and they were much larger than those obtained on Conway medium. Growth parameters of C. calcitrans f. pumilus depended on the ratio of inorganic nitrogen and phosphorus, as well as on the silicon content in nutrient media. The ratio N : P = 12.5 and the silicon concentration of 24 mg·l-1 in the modified F/2 nutrient medium are shown to be approaching the optimal ones for increasing growth rate of diatom algae. It is found that the microalga in concentration 150 × 103 cells·ml-1, cultivated on different nutrient media and included in food composition, has impact on the growth rate of giant oyster larvae. An average daily amount of growth of larvae, whose diet included algae cultivated on modified F/2 nutrient medium, was higher than that of larvae cultivated on Conway medium.

scholarly journals Characterization of a new natural fiber extracted from Corypha taliera fruit

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Taslima Ahmed Tamanna ◽  
Shah Alimuzzaman Belal ◽  
Mohammad Abul Hasan Shibly ◽  
Ayub Nabi Khan
Keyword(s):  
Tensile Strength ◽  
Natural Fiber ◽  
Natural Fibers ◽  
Morphological Characteristics ◽  
Synthetic Fiber ◽  
X Ray Diffraction ◽  
Reinforced Composite ◽  
Potential Alternative

AbstractThis study deals with the determination of new natural fibers extracted from the Corypha taliera fruit (CTF) and its characteristics were reported for the potential alternative of harmful synthetic fiber. The physical, chemical, mechanical, thermal, and morphological characteristics were investigated for CTF fibers. X-ray diffraction and chemical composition characterization ensured a higher amount of cellulose (55.1 wt%) content and crystallinity (62.5%) in the CTF fiber. The FTIR analysis ensured the different functional groups of cellulose, hemicellulose, and lignin present in the fiber. The Scherrer’s equation was used to determine crystallite size 1.45 nm. The mean diameter, specific density, and linear density of the CTF fiber were found (average) 131 μm, 0.86 g/cc, and 43 Tex, respectively. The maximum tensile strength was obtained 53.55 MPa for GL 20 mm and Young’s modulus 572.21 MPa for GL 30 mm. The required energy at break was recorded during the tensile strength experiment from the tensile strength tester and the average values for GL 20 mm and GL 30 mm are 0.05381 J and 0.08968 J, respectively. The thermal analysis ensured the thermal sustainability of CTF fiber up to 230 °C. Entirely the aforementioned outcomes ensured that the new CTF fiber is the expected reinforcement to the fiber-reinforced composite materials.

Influence of tree age and growth rate on the radial resin duct system in loblolly pine (Pinus taeda)

1986 ◽  
Vol 64 (5) ◽  
pp. 1046-1049 ◽  
Author(s):  
J. D. DeAngelis ◽  
T. E. Nebeker ◽  
J. D. Hodges
Keyword(s):  
Growth Rate ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Dendroctonus Frontalis ◽  
Age And Growth ◽  
Tree Age ◽  
Secretory Cells ◽  
Tangential Plane ◽  
Cross Sectional ◽  
Resin Duct

Formation of radial resin ducts and their associated secretory cells in loblolly pine (Pinus taeda L.) is influenced by the age and growth rate of the annual ring in which the ducts are formed. The spatial pattern of radial ducts on the tangential plane is nonrandom, exhibiting a regular or dispersed pattern. A significantly higher density of radial ducts was found in the inner, first-formed growth rings at all heights within the tree. Radial duct formation was found to be positively correlated with radial growth rate, when growth rate is expressed as increment of cross-sectional area growth. These findings may partially explain why older, slower growing trees tend to be more susceptible to attack by the southern pine beetle, Dendroctonus frontalis Zimmermann, and associated microorganisms, since the resin-producing system is a primary defense against these agents.

Determination of the Growth Rate of Hydrothermally Synthesised ZnO2 Crystallites

2017 ◽  
Vol 50 ◽  
pp. 41-47 ◽  
Author(s):  
Martin Egblewogbe ◽  
Garu Gebreyesus ◽  
Samuel A. Atarah
Keyword(s):  
Growth Rate ◽  
Zinc Acetate Dihydrate ◽  
Hydrothermal Process ◽  
Growth Rate Constant ◽  
Acetate Dihydrate ◽  
X Ray ◽  
X Ray Scattering ◽  
Transmission Electron ◽  
Zinc Peroxide

Powders consisting of nanoparticles of zinc peroxide were prepared via a simple hydrothermal process using zinc acetate dihydrate and hydrogen peroxide precursors. The size of the crystallites was determined using x-ray powder diffraction. Over a period of 5 hours the crystallite radius increased from 4 nm – 9 nm at a temperature of 68 °C ± 5 °C, with growth rate constant of 0.23 nm3 min−1 calculated using the Lifshitz, Slyozov, and Wagner model. The powders were further characterised with High Resolution Transmission Electron Microscopy, Energy Dispersive X-ray analysis, and Small Angle X-ray Scattering, showing well-crystallised ZnO2 nanoparticles.

Real-Time Analysis Of In-Situ Spectroscopic Ellipsometric Data During Mbe Growth Of III-V Semiconductors

1991 ◽  
Vol 222 ◽  
Author(s):  
B. Johs ◽  
J. L. Edwards ◽  
K. T. Shiralagi ◽  
R. Droopad ◽  
K. Y. Choi ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Constants ◽  
Growth Parameters ◽  
Ex Situ ◽  
Time Analysis ◽  
Mbe Growth ◽  
In Situ Data ◽  
Real Time Analysis

ABSTRACTA modular spectroscopic ellipsometer, capable of both in-situ and ex-situ operation, has been used to measure important growth parameters of GaAs/AIGaAs structures. The ex-situ measurements provided layer thicknesses and compositions of the grown structures. In-situ ellipsometric measurements allowed the determination of growth rates, layer thicknesses, and high temperature optical constants. By performing a regression analysis of the in-situ data in real-time, the thickness and composition of an AIGaAs layer were extracted during the MBE growth of the structure.

Encasement in plant morphology: an integrative approach from genes to organisms

2005 ◽  
Vol 83 (10) ◽  
pp. 1207-1221 ◽  
Author(s):  
Christian Lacroix ◽  
Bernard Jeune ◽  
Denis Barabé
Keyword(s):  
Growth Rate ◽  
Morphological Characteristics ◽  
Plant Morphology ◽  
Integrative Approach ◽  
Structural Level ◽  
Classical Approach ◽  
Developmental Genes ◽  
Qualitative And Quantitative ◽  
New Concepts ◽  
Dynamic Morphology

Recent advances in molecular genetics are prompting developmental plant morphologists to refine the theoretical context of their field. For example, at the level of the action of certain developmental genes, the distinction between recognized structural categories (i.e., stem and leaf) are not obvious. This issue has also been analyzed by morphologists from qualitative and quantitative perspectives and has lead to similar conclusions. Consequently, the classical approach to morphology with a typological view of organ categories is no longer sufficient to explain the set of all possible forms. However, within the context of a dynamic morphology, where processes of development such as growth rate, duration, and distribution are considered, a more encompassing view of the generation of form can be achieved. We therefore propose that classical morphology is a subset of dynamic morphology. The main goal of this paper is to show how new concepts and methods of viewing plant morphology allow us to build a conceptual theoretical framework that may have a predictive value with respect to morphological characteristics as well as molecular properties of organs. The main premise of this commentary, within the context of dynamic morphology, is that the plant consists of an encasement of structures or a nesting of partially similar units. Common developmental processes are in operation at each structural level and variations in the modalities of these processes lead to the development of specific structures. Repeating polymorphic sets (RPS) represent an extension of this perspective on plant development and have the potential to predict the existence of new, perhaps unknown forms. The idea of repeating polymorphic sets can also be extended to outline the activity of specific developmental genes to explain how a wide variety of those genes are interrelated during development to specify form.

scholarly journals The ameliorative effect of selenium on Azolla caroliniana grown under UV-B stress

2015 ◽  
Vol 95 (1) ◽  
pp. 20-26 ◽  
Author(s):  
E.M. Mostafa ◽  
A.M.A. Hassan
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Doubling Time ◽  
The Other ◽  
Total Glutathione ◽  
Nutrient Media ◽  
Relative Growth ◽  
Other Hand ◽  
Ameliorative Effect ◽  
Glutathione Redox

Exposure ofAzollaplants to UV-B radiation for 6 h resulted in a decrease in biomass and relative growth rate (RGR), which coincided with an increase in doubling time (DT) as compared with the control. Also, the protein content decreased. On the other hand, hydrogen peroxyde (H2O2) and malondialdehyde (MDA) accumulated significantly in UV-treatedAzollaplants. Conversely, the addition of selenium (Se) at 1 ppm resulted in a significant increase in biomass and protein content of untreated and UV-treatedAzollaplants, and a significant reduction in both H2O2and MDA. Moreover, the addition of Se to UV-treated and untreatedAzollaplants resulted in a significant increase in total ascorbate and total glutathione (GSH) contents compared with the control and UV-stressedAzollaplants. Also, glutathione redox potential (GSH/TG) increased significantly in UV-treatedAzollaplants in the presence of Se. There also was a significant increase (38%) in ascorbate peroxidase (APX) activity in UV-treated plants compared with the control. APX activity in the presence of Se did not change significantly compared with the control. Glutathione reductase (GR) activity increased significantly in UV-treatedAzolla, while glutathione peroxidase (GSH-PX) activity did not. On the other hand, both GSH-PX and GR activity in untreated and UV-treatedAzollaplants were significantly enhanced by the application of Se to the nutrient media at a concentration of 1 ppm. Therefore, we can conclude that Se protectsAzollaplants from UV-B stress.

P–311 Association between serum sex hormones (progesterone and estradiol) and endometrial morphology during the mid-luteal phase

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
D Parvanov ◽  
R Ganeva ◽  
M Handzhiyska ◽  
N Vidolova ◽  
G Stamenov
Keyword(s):  
Scoring System ◽  
Luteal Phase ◽  
Morphological Characteristics ◽  
Serum Estradiol ◽  
Serum Progesterone ◽  
Glandular Cells ◽  
Mean Size ◽  
Stromal Edema ◽  
Estradiol Levels

Abstract Study question Is there a relationship between the serum progesterone and estradiol levels and certain morphological characteristics of human endometrium during the mid-luteal phase? Summary answer Serum progesterone is associated with the stromal edema and the abundance and size of basal vacuoles in the endometrium of women during the mid-luteal phase. What is known already Progesterone and estrogen are essential hormones that are necessary to prepare the endometrium for pregnancy. Their serum concentrations during the mid-luteal phase are important criteria for prediction of successful embryo implantation. In addition, a variety of endometrial morphological markers, such as the presence of pinopodes, subnuclear and supranuclear vacuoles, glandular secretion, and stromal edema have been applied for determination of the window of implantation and endometrial receptivity. However, the relationship between these endometrial morphological characteristics and serum levels of progesterone and estradiol is still scarcely studied. Study design, size, duration This is an observational study of 98 women, 25 to 46 years of age (mean 37 years), who had a blood sample and an endometrial biopsy during the mid-lutheal phase (LH + 7) in a natural cycle. The study was conducted between August 2020 and November 2020. Participants/materials, setting, methods Serum progesterone and estradiol were measured by electrochemiluminescence immunoassay (ECLIA) on the Cobas e411 analyser (Roche Diagnostics, Germany). The following endometrial morphological characteristics were assessed using light microscopy: (1) basal vacuoles (mean size and percentage of vacuolated glandular cells) (2) apical vacuoles (mean size and percentage of vacuolated glandular cells), (3) pinopodes (percentage of luminal epithelium covered in pinopodes), (4) glandular intraluminal secretion (6-level scoring system), (5) stromal edema (6-level scoring system). Main results and the role of chance The serum progesterone levels ranged between 0.39 and 145.3 ng/ml, with a median of 24.36 ng/ml. The serum estradiol levels varied between 26.91 and 842.89 pg/ml with a median of 124.75 pg/ml. The percentage of cells with basal vaculoles ranged from 0 to 90%, with a median of 38.57%, apical vacuoles (0–50%, 16.83%), pinopodes (0–80%, 23.87%), glandular intraluminal secretion (0–80%, 28.57%), and stromal edema (1–6, 1.42). To examine the association between the serum progesterone and estradiol and the studied endometrial morphological characteristics, the Spearman’s Rho Correlation coefficient for non-paramentric data was used. No correlation was found between serum estradiol levels and the studied morphological variables (p &gt; 0.05). In contrast, the serum progesterone concentration showed a significant negative correlation with the percentage of glandular epithelial cells with basal vacuoles (R= - 0.28; p = 0.03), the mean size of the basal vacuoles (R= - 0.24; p = 0.5) and a significant positive correlation with the stromal edema (R = 0.34; p &lt; 0.01). Limitations, reasons for caution The study was limited in sample size. Wider implications of the findings: The results of this study revealed that serum progesterone is more strongly associated with the occurrence of certain endometrial morphological characteristics during the mid-luteal phase than serum estradiol. These findings are valuable for development of new methods for accurate determination of the window of implantation. Trial registration number Not applicable

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