scholarly journals Antibiotic susceptibilty of bacteria from the Bacillus subtilis group

2019 ◽  
Vol 9 (3) ◽  
pp. 363-366
Author(s):  
A. N. Irkitova ◽  
A. Vu. Grebenshchikova ◽  
D. E. Dudnik
Keyword(s):  
Antibiotic Resistance ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Beneficial Microbes ◽  
Global Problem ◽  
Highly Sensitive ◽  
Bacillus Spp ◽  
Subtilis Group

The ability of bacteria to quickly acquire antibiotic resistance and transfer it among different groups are created a global problem, since antibiotics are used not only in medicine, but also in agriculture. That is why to study of the antibiotic sensitivity of not only pathogenic bacteria, but also of beneficial microbes like Bacillus spp. is extremely important. Our results demonstrated that all the studied strains of the B. subtilis group were highly sensitive to cephalexin and enrofloxacin, while resistant to oxacillin. The B. subtilis B-5449 strain was the least resistant to the studied antibiotics, and the B. subtilis B-2896 strain was the most resistant.

scholarly journals Structural basis for antibiotic resistance mediated by the Bacillus subtilis ABCF ATPase VmlR

2018 ◽  
Vol 115 (36) ◽  
pp. 8978-8983 ◽  
Author(s):  
Caillan Crowe-McAuliffe ◽  
Michael Graf ◽  
Paul Huter ◽  
Hiraku Takada ◽  
Maha Abdelshahid ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Bacillus Subtilis ◽  
Binding Site ◽  
Pathogenic Bacteria ◽  
Small Subunit ◽  
Structural Basis ◽  
Abc Protein ◽  
Peptidyltransferase Center ◽  
A Site ◽  
Microbiological Analyses

Many Gram-positive pathogenic bacteria employ ribosomal protection proteins (RPPs) to confer resistance to clinically important antibiotics. In Bacillus subtilis, the RPP VmlR confers resistance to lincomycin (Lnc) and the streptogramin A (SA) antibiotic virginiamycin M (VgM). VmlR is an ATP-binding cassette (ABC) protein of the F type, which, like other antibiotic resistance (ARE) ABCF proteins, is thought to bind to antibiotic-stalled ribosomes and promote dissociation of the drug from its binding site. To investigate the molecular mechanism by which VmlR confers antibiotic resistance, we have determined a cryo-electron microscopy (cryo-EM) structure of an ATPase-deficient B. subtilis VmlR-EQ2 mutant in complex with a B. subtilis ErmDL-stalled ribosomal complex (SRC). The structure reveals that VmlR binds within the E site of the ribosome, with the antibiotic resistance domain (ARD) reaching into the peptidyltransferase center (PTC) of the ribosome and a C-terminal extension (CTE) making contact with the small subunit (SSU). To access the PTC, VmlR induces a conformational change in the P-site tRNA, shifting the acceptor arm out of the PTC and relocating the CCA end of the P-site tRNA toward the A site. Together with microbiological analyses, our study indicates that VmlR allosterically dissociates the drug from its ribosomal binding site and exhibits specificity to dislodge VgM, Lnc, and the pleuromutilin tiamulin (Tia), but not chloramphenicol (Cam), linezolid (Lnz), nor the macrolide erythromycin (Ery).

scholarly journals ANTIBIOTIC SENSITIVITY OF CLINICAL ISOLATES AT OUTPATIENT UNITIN TVER, RUSSIA: A COMPARATIVES

2020 ◽  
Vol 10 (4) ◽  
pp. 77-79
Author(s):  
Konstantin Horak ◽  
Kirill Gorodnichev ◽  
Artem Morozov ◽  
Sergey Zhukov ◽  
Margarita Rybakova ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Statistical Analysis ◽  
General Trend ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Case Record ◽  
New Era ◽  
Clinical Specimens ◽  
Bacterial Diseases ◽  
Highly Sensitive

Background Discovery of antibiotics opened a new era in the treatment of bacterial diseases. However, the microorganisms are able to adapt and resist the effects of the drugs. Objective Our study is aimed to investigate the sensitivity of clinical specimens to antibiotics. Methods Clinical samples of 280 case record forms were collected at departments of surgery, urology and otorhinolaryngology in an ambulatory clinic (Tver, Russia) during 2019. The results of microbiologically assessed isolates from pharynx, nose, ears, eyes, wounds, sputum and urine underwent statistical analysis. Results The outcomes confirmed a general trend of reduced susceptibility of bacteria to antibiotics. The worst result was shown by protected Amoxicillin; practically no microorganisms were sensitive to it. Protected Cephalosporins Cephalosporins of IV generation and Imipenem were among the best, although not highly sensitive to all the pathogens. Conclusion United efforts of all states are required to combat the growing antibiotic resistance. It is necessary to adhere to strict regulations on dispense of antibiotics in pharmacies and the use of antibiotics therapy

Effect of metals on antibiotic sensitivity, growth, and biofilm-forming capacity of B. subtilis subsp. spizizenii

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Selma Cifrić
Keyword(s):  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Antibiotic Sensitivity ◽  
Probiotic Strain ◽  
Adsorptive Capacity ◽  
Metal Compounds ◽  
Bacillus Spp ◽  
Probiotic Strains ◽  
Bacteria Culture ◽  
Subtilis Subsp

B. subtilis is normally considered a soil organism, it can be also found in the animal and human gastrointestinal tract. Bacillus subtilis subsp. spizizenii is a type of Bacillus subtilis complex. It shares up to 99% of homology with B. subtilis CU1, which can be represented as a probiotic strain. Metal compounds found in soil or used in agriculture can easily enter the food chain and end up in our gut. Gram-positive bacteria (e.g. Bacillus spp.) have good adsorptive capacity for metals due to high peptidoglycan and teichoic acid content in cell walls. There is some evidence that certain metals inside the intestine play an important role in influencing growth and functionality of specific probiotic strains. Some of them have inhibitory, while others have an activating effect on bacteria. This study revealed that metal compounds increased antibiotic susceptibility of B. subtilis subsp. spizizenii. Higher concentrations of metal solutions inhibited growth of tested bacteria. Culture did not show affinity to form biofilms before or after addition of metal solutions.

STUDY ON ANTIBIOTIC RESISTANCE OF COMMON PATHOGENIC BACTERIA AT BINH AN HOSPITAL IN KIEN GIANG IN 2010

2013 ◽  
pp. 35-42
Author(s):  
Nguyen Ngoc Trac Mai
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Antibiotic Use ◽  
Beta Lactamase ◽  
Cross Sectional ◽  
Highly Sensitive ◽  
Klebsiella Spp ◽  
Lactamase Inhibitor

Objective: Study on the distribution of common pathogens at Binh An hospital in 2010 and their antibiotic resistance. Methods: Retrospective, descriptive and cross-sectional methods were used. Data of bacterial identification and antibiogram results were collected at Binh An hospital from January to December 2010. Results: The top 5 bacterias were E.coli (33.93%), Streptococcus spp. (23.21%), Staphylococcus aureus (14.29%), Klebsiella pneumoniae (8.93%) and Pseudomonas aeruginosa (7.14%). E.coli strains were high resistant to Ampicillin (100%), Ticarcillin (100%), Trimethoprim/Sulfamethoxazol (85%) and highly sensitive to Imipenem (94%), Cefoperazone/Sulbactam (93%) and Piperacillin/Tazobactam (83%). Resistant rates for Streptococcus spp. were as follows: Oxacillin (100%), Gentamicin (77%), Amikacin (77%), Trimethoprim/Sulfamethoxazol (62%). Streptococcus spp. were sensitive to Vancomycin (100%), Imipenem (100%), Piperacillin and Cefoperazone/Sulbactam (100%). Staphylococcus aureus were high sensitive to Vancomycin (100%) and combinations of Betalactam/Beta-lactamase inhibitor (100%). Carbapenems and combinations of Betalactam/Beta-lactamase inhibitor were effective to Klebsiella spp. Imipenem is still a realistic selection for Pseudomonas aeruginosa Conclusion: Continuous surveillance of antibiotic resistance as well as reasonable antibiotic use are required to mitigate the progression of antibiotic resistance. Key words: antibiotic, common pathogens

scholarly journals Bacillus subtilis-Mediated Protection from Citrobacter rodentium-Associated Enteric Disease RequiresespHand Functional Flagella

2011 ◽  
Vol 80 (2) ◽  
pp. 710-719 ◽  
Author(s):  
Sara E. Jones ◽  
Katherine L. Knight
Keyword(s):  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Cell Loss ◽  
Enteric Infection ◽  
Citrobacter Rodentium ◽  
Human Pathogens ◽  
Wild Type ◽  
Content Type ◽  
Beneficial Microbes ◽  
Pathogen Colonization

ABSTRACTCommensals limit disease caused by invading pathogens; however, the mechanisms and genes utilized by beneficial microbes to inhibit pathogenesis are poorly understood. The attaching and effacing mouse pathogenCitrobacter rodentiumassociates intimately with the intestinal epithelium, and infections result in acute colitis.C. rodentiumis used to model the human pathogens enterohemorrhagicEscherichia coliand enteropathogenicE. coli. To confirm thatBacillus subtilis, a spore-forming bacterium found in the gut of mammals, could reduceC. rodentium-associated disease, mice received wild-typeB. subtilisspores and 24 h later were infected by oral gavage with pathogenicC. rodentium. Disease was assessed by determining the extent of colonic epithelial hyperplasia, goblet cell loss, diarrhea, and pathogen colonization. Mice that received wild-typeB. subtilisprior to enteric infection were protected from disease even thoughC. rodentiumcolonization was not inhibited. In contrast,espHandhagmutants, defective in exopolysaccharides and flagellum production, respectively, did not protect mice fromC. rodentium-associated disease. AmotABmutant also failed to protect mice from disease, suggesting thatB. subtilis-mediated protection requires functional flagella. By expanding our current mechanistic knowledge of bacterial protection, we can better utilize beneficial microbes to prevent intestinal disease caused by pathogenic bacteria, ultimately reducing human disease. Our data demonstrate that wild-typeB. subtilisreduced disease caused byC. rodentiuminfection through a mechanism that requiredespHand functional flagella.

scholarly journals Antibiotic resistance pattern of bacteria isolated from outdoor patients in Dhaka city: a single center study

2020 ◽  
Vol 9 (1) ◽  
pp. 1-4
Author(s):  
Most Salma Khatun ◽  
Saimun Nahar ◽  
Md Shahidul Kabir
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Age Groups ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Dhaka City ◽  
Female Patients

Bacterial infections are often found to cause morbidity and mortality around the globe. Indiscriminate use of antibiotic for treatment of such infections is reported to cause selective pressure and increase in drug resistance. Emergence of antibiotic resistance is a growing concern for people of all age having bacterial infections. It is important to determine the trend of antibiotic resistance patterns of pathogenic bacteria isolated from clinical samples for appropriate treatment of patients. A retrospective study was conducted on patient samples collected from 1 July 2018 to 15 March 2019. A total of 500 urine, 136 blood, 120 stool, 172 swab, 90 Sputum and 60 pus samples were randomly collected from both male and female patients of different age groups who reported to a diagnostic centre in Dhaka city. Pathogenic bacteria were isolated and identified from the collected samples following standard methods. Antibiotic sensitivity patterns of isolated bacteria were also determined by disc diffusion test. Proportion of UTI in female patients (71.67%) was found to be higher than the male (28.33%) patients and mostly caused by Escherichia coli (73.33%). Among Gram negative bacteria, E. coli (51%) was found to be predominant followed by Pseudomonas spp. (11.47%), Klebsiella (9.84%) and Salmonella Typhi (9.84%). Among Gram positive organism Staphylococcus aureus (9.0%), beta haemolytic streptococci (5.74%) and enterococci (3.28%) were found to be present. Among the tested antibiotics imipenem was found to be the most effective (93.02%) followed by gentamycin (76.03%) against all isolated bacteria. Amoxycillin was found to be least effective (21.29%) against all isolated bacteria. Increase in antibiotic resistance was possibly due to indiscriminate use of antibiotic for treatment of various infections. Prudent use of antimicrobial drugs will help reduce spread of resistant bacteria and complication of treatment of infected patients. Stamford Journal of Microbiology, Vol.9(1) 2019: 1-4

scholarly journals The Biological Activity of Some Extracted Proteins from Bacillus spp. Isolated from Soil Against Pathogenic Bacteria

2020 ◽  
Vol 30 (4) ◽  
pp. 29
Author(s):  
Haneen Ali Ibrahim ◽  
Neihaya Heikmat Zaki
Keyword(s):  
Breast Cancer ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Cytotoxic Effect ◽  
Pathogenic Bacteria ◽  
Polyacrylamide Gel Electrophoresis ◽  
Extracellular Proteins ◽  
Mcf7 Cells ◽  
Bacillus Spp

Forty soil samples were collected from different regions in Baghdad city. Among 52 isolate, only 38 isolates (73.1 %) belong to Bacillus spp. All bacterial isolates have been submitted to the cultural, microscopical, biochemical examination and VITEK 2 System, Bacillus cereus was predominantly found 21 (55.3%) followed by Bacillus subtilis 11 (28.9%), then Bacillus amyloliquefaciens was 6 (15.8%). Bacillus cereus (B1) and Bacillus subtilis (B16) isolates were with the highest level with the antibacterial activity when selected. Extracellular proteins were extracted and purified with ammonium sulfate (80%) saturation, then submitted to Sepharose-6B gel filtration column. SDS-polyacrylamide gel electrophoresis was used to determine the molecular weights of proteins, and one band appeared to each of Bacillus cereus and Bacillus subtilis with molecular weight 68 and 50 KDa respectively. The results revealed that higher effect of antibacterial activity on Gram positive bacteria, and moderate on Gram negative bacteria except P.aeruginosa with no effect. The antibiofilm effect of the extract found higher effective against S.pyogens, moderate effect against each of (S.aureus, E.coli, S. marcescens, K. spp) and no effect against P.aeruginosa. The results of cytotoxicity showed that the purified extract was a higher cytotoxic effect on breast cancer MCF7 cells at 1000µg/ml concentration, while the crude extract had a higher cytotoxic effect on breast cancer MCF7 cells at 100µg/ml concentration.

PHÂN LẬP VÀ TUYỂN CHỌN CÁC CHỦNG BACILLUS ỨC CHẾ VI KHUẨN VIBRIO PARAHAEMOLYTICUS GÂY BỆNH TÔM CHẾT SỚM Ở TỈNH THỪA THIÊN HUẾ

2015 ◽  
Vol 100 (1) ◽  
Author(s):  
Nguyễn Thị Bích Đào ◽  
Trần Quang Khánh Vân ◽  
Nguyễn Văn Khanh ◽  
Nguyễn Quang Linh
Keyword(s):  
Bacillus Subtilis ◽  
Vibrio Parahaemolyticus ◽  
Bacillus Amyloliquefaciens ◽  
Bacillus Spp ◽  
Bacillus Subtilis B1

Khi tình hình bệnh hội chứng tôm chết sớm (EMS) đã gây thiệt hại vô cùng to lớn đối với Nuôi trồng thủy sản thì các giải pháp được đề nghị và áp dụng nhằm hạn chế dịch bệnh. Trong đó, việc tìm hiểu và đưa vi khuẩn có lợi để cạnh tranh và ức chế loài vi khuẩn gây bệnh rất được quan tâm, được cho là giải pháp có nhiều triển vọng phù hợp với điều kiện môi trường, đảm bảo sức khỏe cho con người, cũng như hạn chế được dịch bệnh. Đặc biệt, đưa vi khuẩn Bacillus spp. qua đường tiêu hóa của tôm ngay từ khi mới thả đã hạn chế được mật độ vi khuẩn Vibrio. Nghiên cứu này đã phân lập được các chủng Bacillus subtilis B1, Bacillus subtilis B2, Bacillus amyloliquefaciens B4và thử khả năng đối kháng với vi khuẩn Vibrio parahaemolyticus V1 ở các nồng độ 103, 104, 105, 106 CFU theo dõi ở các thời điểm 6h, 12h, 24h, 48h và 72h. Kết quả cho thấy cả ba chủng vi khuẩn Bacillus trên phân lập được đều có khả năng ức chế tốt vi khuẩn Vibrio parahaemolyticus V1, trong đó vi khuẩn Bacillus amyloliquefaciens B4 làtốt nhất với đường kính vòng kháng khuẩn 52,67 ± 4,31mm ở thời điểm 48h; hai chủng Bacillus subtilis B1, Bacillus subtilis B2 lầnlượt là  49,67 ± 3,15 mm, 44,07 ± 5,19 mm, với mức sai số có ý nghĩa thống kê p < 0,05.

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

scholarly journals Effect of Sulfonamides and Their Structurally Related Derivatives on the Activity of ι-Carbonic Anhydrase from Burkholderia territorii

2021 ◽  
Vol 22 (2) ◽  
pp. 571
Author(s):  
Viviana De Luca ◽  
Andrea Petreni ◽  
Alessio Nocentini ◽  
Andrea Scaloni ◽  
Claudiu T. Supuran ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Selective Inhibition ◽  
Extensive Study ◽  
Protein Isoforms ◽  
Carbonic Anhydrases ◽  
Physiological Processes ◽  
Microbial Survival ◽  
Reversible Hydration

Carbonic anhydrases (CAs) are essential metalloenzymes in nature, catalyzing the carbon dioxide reversible hydration into bicarbonate and proton. In humans, breathing and many other critical physiological processes depend on this enzymatic activity. The CA superfamily function and inhibition in pathogenic bacteria has recently been the object of significant advances, being demonstrated to affect microbial survival/virulence. Targeting bacterial CAs may thus be a valid alternative to expand the pharmacological arsenal against the emergence of widespread antibiotic resistance. Here, we report an extensive study on the inhibition profile of the recently discovered ι-CA class present in some bacteria, including Burkholderia territorii, namely BteCAι, using substituted benzene-sulfonamides and clinically licensed sulfonamide-, sulfamate- and sulfamide-type drugs. The BteCAι inhibition profile showed: (i) several benzene-sulfonamides with an inhibition constant lower than 100 nM; (ii) a different behavior with respect to other α, β and γ-CAs; (iii) clinically used drugs having a micromolar affinity. This prototype study contributes to the initial recognition of compounds which efficiently and selectively inhibit a bacterial member of the ι-CA class, for which such a selective inhibition with respect to other protein isoforms present in the host is highly desired and may contribute to the development of novel antimicrobials.

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