Hormones concentration in the blood of dogs with implant of the Suprelorin 4.7 mg

This article describes the results of the study of the prostate gland of dogs and changes in the level of sex hormones when treated with Suprelorin 4.7 mg implanted subcutaneously in the shoulder region. Suprelorin is an innovative drug that limits the production of testosterone. His action can be compared with the results of castration, but the owners of the animal provide their dog and, in fact, themselves, with the lack of stress and various problems associated with surgical intervention. Suprelorin is a non-steroidal, peptide contraceptive in the form of an implant. The active ingredient is deslorelin (an agonist GnRH), which inhibits the reproductive function at the level of the hypothalamic-pituitary axis for at least 6 months. Deslorrelin is a synthetic analogue of GnRH less susceptible to decomposition, seven times more potent than endogenous GnRH, more stable, and also with a higher sensitivity to GnRH receptors. GnRH plays a key role in controlling the reproductive system of females and males. And in the case of long-term prescribing of small doses of deslorelin, inhibition of functioning of the pituitary gonadal axis occurs. The central place in which the blocking of the synthesis and / or allocation of follicle stimulating (FSH) and luteinizing hormones (LH) in the dogs, which was introduced implant. In this case, there are no side effects of steroid. When conducting diagnostic measures with the help of an ultrasound machine, the tasks facing the specialist are: to determine the shape, size and echostructure of the prostate gland, its contours and capsules. According to the results of the ultrasound, experimental groups of animals and control were formed. The criterion for the formation of experimental groups was the increase the size of the prostate and structural changes (increasing or decreasing echogenicity, hypo-and anechoic inclusion in the parenchyma) in it. In the examination of dogs by ultrasound, it was found that the animals in the control group of the prostate were normal, and in animals of the experimental group were found structural changes in it, in particular, increase the size of the gland, increase echogenicity and heterogeneity of the structure of the parenchyma, anechoic inclusions of various sizes. While assessing the level of hormones in dogs, there was a significant decrease in testosterone levels in the experimental group, as well as an increase in the level of estradiol, luteinizing hormone, thyroid stimulating hormone, and T3 total in blood serum of dogs in experimental groups. The level of hormones was monitored weekly and reflected its changes on the schedule. Libido of all animals under the action of the implant significantly decreased. Behavior became calmer. Appetite improved and activity was normal. The sexual function of the treated dogs was completely restored a year later.

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EFFECT OF PVMC DEADWOOD AND OF PVMC LACTATION ON THE COW REPRODUCTION

Molochnoe i miasnoe skotovodstvo ◽

10.33943/mms.2021.11.38.005 ◽

2021 ◽

pp. 22-27

Author(s):

О.В. ЛАТЫШЕВА ◽

А.В. ИВАНОВ

Keyword(s):

Essential Oils ◽

Plant Extracts ◽

Average Duration ◽

Reproductive Function ◽

Feed Additive ◽

Control Group ◽

Economic Experiment ◽

Service Period ◽

Experimental Group

Изучено влияние на репродуктивную функцию коров белково-витаминно-минеральных концентратов (БВМК) с кормовой добавкой «AMG-COMPLEX», состоящей из эфирных масел и растительных экстрактов. Для проведения опыта были сформированы контрольная и опытная группы по 70 голов с учетом количества дней до отела из коров второй и третьей стельности. Животным контрольной группы скармливали основной рацион. Для коров опытной группы были составлены рационы с БВМК, которыми заменяли пропорциональное количество концентрированных кормов и часть витаминно-минерального премикса. Они получали БВМК для сухостойных коров в количестве 300 г на 1 голову в сутки с 60-го по 20-й день до отела и по 200 г на 1 голову в сутки с 20-го дня до отела. После отела 60 дней им скармливали БВМК для раздоя в количестве 1200 г на 1 голову в сутки. Использование БВМК в рационах коров опытной группы способствовало улучшению у них репродуктивной функции и снижению частоты гинекологических заболеваний после отела в 4,5 раза. Индекс осеменения в опытной группе составил 1,73 (P≤0,05), что на 0,76 ниже, чем у животных контрольной группы. Средняя продолжительность сервис-периода в опытной группе составила 87,71 дня (P≤0,01) и была короче, чем в контроле, на 14,72 дня. The effect on the reproductive function of cows of the protein-vitamin-mineral concentrate with the feed additive ʺAMG-COMPLEXʺ, consisting of essential oils and plant extracts, was studied. To experiment, taking into account the number of days before calving, control and experimental group of 70 heads each were formed from cows of the second and third pregnancy. In the scientific and economic experiment, the cows of the control group consumed the feed of the main rations. For the cows of the experimental group rations were made with PVMC, which replaced the proportional amount of concentrated feed and a part of the vitamin-mineral premix. They received PVMC deadwood in the amount of 300 g per 1 head per day from 60th to 20th day before calving and 200 g per 1 head per day 20 days before calving. 60 days after calving they received PVMC lactation in the amount of 1200 g per head per day. Feeding PVMC to cows of the experimental group contributed to the improvement of reproductive function and a decrease in the frequency of gynaecological diseases after calving by 4.5 times. The insemination index in the experimental group was 1.73 (P≤0.05), which is 0.76 lower than in the animals in the control group. The average duration of the service period in the experimental group was 87.71 days (P≤0.01) and was shorter than in the control by 14.72 days.

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Comparison of Urinary Iodine, Urinary Arsenic, Radioiodine Uptake, Thyroid Stimulating Hormone (TSH) and Free Thyroxin (FT4) Levels, Between Experimental Group with Simple Diffuse Goiter and Control Group

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v7i1.1224 ◽

1970 ◽

Vol 7 (1) ◽

pp. 89-98 ◽

Cited By ~ 1

Author(s):

Md Sharif Hossain ◽

Fatema Begum

Keyword(s):

Urinary Iodine ◽

Thyroid Stimulating Hormone ◽

Control Group ◽

Control Groups ◽

Arsenic Level ◽

Radioiodine Uptake ◽

Urinary Arsenic ◽

Diffuse Goiter ◽

And Control ◽

Experimental Group

In Bangladesh some researchers have done several studies to find the correlation of simple diffuse goiter with arsenic level but no one conducted any study to find the differences of urinary iodine, urinary arsenic, radioiodine uptake, the thyroid stimulating hormone (TSH) and free thyroxin (FT4) levels between goitrous and non goitrous. That is why, in this research an attempt has been made to compare the urinary iodine, urinary arsenic, radioiodine uptake, TSH and FT4 levels between experimental group with simple diffuse goiter and healthy control group. In this study another purpose has also been made to find the associations between different pairs of variables for both experimental and control group. In this study we have tried to find the impacts of arsenic level on simple diffuse goiter. This is a case-control analytical study. The study is carried out in the Institute of Nuclear Medicine and Ultrasound, in collaboration with the thyroid out patient department of Bangabandhu Sheikh Mujib Medical University. A total of eighty five (85) subjects are included in the study. Among the 85 subjects, a sample of forty five is considered for experimental group with simple diffuse goiter and another sample of forty is considered for healthy control group without any thyroid disease. From the experimental results it has been found that, the arsenic levels, FT4 and radioiodine uptake levels at 2 hours between the experimental and control groups are significantly different but the urinary iodine levels, TSH levels and radioiodine uptake levels at 24 hours between the experimental and control groups are not statistically different. From the experimental results it has also been found that there is significant differentiation between experimental and controls groups in respect of association between different pairs of variables. In this study, another significant finding is that iodine deficiency is not only the factor of causing simple diffuse goiter, but arsenic level is also one of the most important factor of causing simple diffuse goiter. Key words: Iodine deficiency, Simple diffuse goiter, Urinary arsenic, Statistical analysis, Association, Experimental and Control groups Â DOI = 10.3329/dujps.v7i1.1224 Dhaka Univ. J. Pharm. Sci. 7(1): 89-98, 2008 (June)

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Екологічні аспекти обміну Мангану у високопродуктивних корів у перші 100 днів лактації за згодовування їм змішанолігандних комплексів Цинку, Мангану і Кобальту

Ukrainian Journal of Ecology ◽

10.15421/2018_230 ◽

2018 ◽

Vol 8 (1) ◽

pp. 420-425 ◽

Cited By ~ 2

Author(s):

V. Bomko ◽

Yu. Kropyvka ◽

L. Bomko ◽

S. Chernyuk ◽

S. Kropyvka ◽

...

Keyword(s):

Trace Elements ◽

Potassium Iodide ◽

Reproductive Function ◽

Mixed Ligand ◽

The Body ◽

Mixed Ligand Complexes ◽

Fecal Excretion ◽

Control Group ◽

Milk Productivity ◽

Experimental Group

To realize the genetic potential of animals, an important role should be taken to prevent the presence of mineral substances in the rations. Because of their lack of revenue in the body of animals the productivity is decreased, the reproductive function is disturbed, there is a disease. there are diseases.Material for the scientific and economic experiment on the use of mixed ligand complexes of Zinc, Manganese and Cobalt on the milk productivity of high-yielding cows and the exchange of Mangan in their body were Holstein animals, Ukrainian Black- Spotted dairy and Ukrainian Red - Spotted dairy breeds. The trace elements were introduced into feed concentrates, and they into the fodder mix by the method of weight dosing and multi-stage mixing. Balancing of rations of highly productive cows of the 1st control group to norm only Selenium, Kuprum and Iodine, led to a concentration in 1 kg DM (dry matter) of feed mix, mg: Zinc – 32.4; Manganese – 27.8; Cobalt – 0.27; Selenium – 0.3; Copper – 12 and Iodine – 1.1 and the lowest concentration of Mangan in milk and in the body. The cows of the 2nd and 3rd experimental groups were fed with all the trace elements to the norm (for the 2nd experimental group at the expense of zinc sulfates, manganese, cobalt, copper, selenium, Suplex of Selenium and potassium iodide, for the 3rd instead of zinc sulfates, manganese and mixed cobalt compounds were introduced into their ligand complexes). The concentration of trace elements in these groups in 1 kg of DM was, mg: Zinc – 76; Manganese – 76; Cobalt – 0,97; Copper – 12; Selenium – 0.3 and Iodine – 1.1. The concentration of Zinc, Manganese and Cobalt in 1 kg of DM of fodder was reduced by 20% for cows of the 4th experimental group and by 30% for the 5th experimental group.The results of the conducted searches indicate that the use in feeding of high-yielding cows of various doses of mixed ligand complexes of Zinc, Manganese, and Cobalt on the use of Suplex of Selenium and sulfate copper and potassium iodide in the first 100- days lactation positively affect the growth of their milk production. Animals of experimental groups dominated cows of analogues of the control group, rations of which were scarce on Zinc, Manganese and Cobalt, by average daily yield of natural milk, respectively, by 4.5; 5.8 and 4.0 kg, or 11.63: 14.99 and 10.34%, and these differences were reliable. While the average daily milk yield of cows of the 2nd experimental group exceeded the average daily yield of the 1st control group by 3.8 kg or by 9.82%. In milk of experimental cows, a single increase in the fat content of 0.01–0.09% was also noted. Carrying out balance research confirmed the data of milk productivity. Thus, in the body of cows of the 3rd, 4th and 5th experimental groups, more Manganese were deposited, respectively by: 540.7; 531.5, and 394.5 mg compared to control and by 367.1, 357.9, and 220.9 mg – in comparison with the 2nd experimental group; in experimental cows of groups 4 and 5, the manganese was deposited less by 9.2 and 146.2 mg in comparison with the 3rd experimental group. With milk in cows of the 1st control group during the day was allocated 20.8 mg of Manganese, and in the 2nd, 3rd, 4th and 5th experimental groups it was allocated more, respectively by 6.7, 9.8, 15.4, and 10.6 mg. Manganese fecal excretion was less in comparison with the 2nd experimental group, in the cows of the 3rd experimental group it was less by 365.9 mg, in the 4th group – by 623.3 mg, and in the 5th group – by 691.4 mg.The use of mixed ligand complexes of Zinc, Manganese and Cobalt enhances the milk productivity of high-yielding cows, reduces their need for these elements, improves their digestion and reduces their excretion with feces.

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PATHOGENETIC EFFECTS OF COMBINATION THERAPY IN THE TREATMENT OF COMORBID PATHOLOGY

Likarska sprava ◽

10.31640/jvd.4.2019(5) ◽

2019 ◽

pp. 29-35

Author(s):

P. Herych ◽

I. Popadynets ◽

R. Yatsyshyn ◽

V. Mygovych ◽

V. Kaminskyi

Keyword(s):

Nitric Oxide ◽

Structural Changes ◽

Patient Treatment ◽

Control Group ◽

Chronic Obstructive ◽

Peripheral Arteries ◽

Mean Values ◽

Stable Coronary Heart Disease ◽

Complex Therapy ◽

Experimental Group

The objective of the work was to determine the possibility of drug correction of endothelial dysfunction, structural changes in peripheral arteries and the synthesis of end products of nitric oxide metabolism by the use of a combination of roflumilast and quercetin in the baseline therapy. In contrast to the baseline, the use of integrated therapy was followed in 6 months by a probable increase in the initial rate by 18.76 % (0.61 ± 0.04) m/s to (0.75 ± 0.04) m/s (t = 2.47; P < 0.05) and a probable decrease in the initial diameter of the brachial artery to (3.69 ± 0.29) mm (t = 2.49; P < 0.05). Patients of the experimental group have shown a significant increase in mean values of EDVD at the end of in-patient treatment and after 6 months of intensive supportive therapy (t = 2.17; Р < 0.05). The appointment of complex therapy after 6 months showed an increase in concentration in the blood of metabolites of NO in 1,3 times to (10.35 ± 1.89) μmol/l (t = 1.00; P > 0.1) at normal (12.05 ± 2.11) μmol/l. In patients of the index group after six months of the background therapy, IMT index has appeared in 1.07 times lower than the input data and has not reached the level of the control group. The IMT index for patients in the experimental group was considerably lower than the index before treatment (t = 0.31; P > 0.1). Application of complex therapy in patients with severe chronic obstructive pulmonary disease in the exacerbation phase in combination with stable coronary heart disease, stable angina pectoris I–II FK contributes to the restoration of endothelial function, improves structural changes in the peripheral arteries and has a stimulating effect on the synthesis of nitric oxide.

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Effect of the prenatal action of fulvestrant on the ovaries of the offspring of laboratory mice

RUDN Journal of Medicine ◽

10.22363/2313-0245-2021-25-3-256-262 ◽

2021 ◽

Vol 25 (3) ◽

pp. 256-262

Author(s):

Rimma T. Sulaymanova

Keyword(s):

Structural Changes ◽

Reproductive Function ◽

Follicular Epithelium ◽

Cystic Degeneration ◽

Control Group ◽

Laboratory Mice ◽

Primordial Follicles ◽

Stromal Component ◽

Gestational Stage ◽

Dose Dependent

Relevance. Fulvestrant is used for the treatment of breast cancer in combination with other drugs. The aim of the study was to determine the effect of the prenatal action of fulvestrant on the ovaries of the offspring of laboratory mice. Materials and Methods. The experimental animals were divided into 4 groups: intact, control and 2 experimental, 5 animals in each group. Injections were administered to females after fertilization at the gestational stage E 11.5 once intramuscularly. In the control group (n=5), sterile castor oil was administered at a dose of 0.8 mcg/kg. In the first experimental group (n=5), an antiestrogen was introduced in the form of an oil solution of fulvestrant 0.08 ml 0.0005% at a dose of 20 mcg/kg. In the second group (n=5), an antiestrogen was introduced in the form of an oil solution of fulvestrant 0.4 ml 0.0005% at a dose of 100 mcg/kg. Results and Discussion . The study revealed that in the ovaries when the drug was administered at a dose of 20 mcg/kg (F-20), the number of primordial follicles was reduced. Accordingly, the number of follicles of subsequent generations decreased. With the introduction of the drug fulvestrant 100 mcg/kg (F-100) on the section of the ovary, sclerosis of the stromal component is observed, accompanied by a rearrangement of the vascular network with signs of atresia and cystic degeneration of the follicular epithelium in the secondary and tertiary follicles, formed cysts are observed in the ovarian parenchyma. Conclusion. The prenatal effect of the drug fulvestrant on the maternal body during pregnancy leads to persistent structural changes in the ovaries of the offspring, manifested in the late stages of ontogenesis, which, in turn, can lead to violations of reproductive function. The depth and scale of these changes are dose-dependent.

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Hematological parameters and incubation quality of eggs for different levels of tryptophan in the diet of geese of the parent flock

Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies ◽

10.32718/nvlvet-a9018 ◽

2019 ◽

Vol 21 (90) ◽

pp. 104-107

Author(s):

V. M. Volovich ◽

S. A. Vovk

Keyword(s):

Hematological Parameters ◽

White Blood Cells ◽

Reproductive Period ◽

Feed Additives ◽

Control Group ◽

Egg Mass ◽

Synthetic Analogue ◽

Important Indicator ◽

Experimental Group

The aim of the work was comparative study of the effect of various amounts of tryptophan in the diet of the herds geese on changes in the parameters of protein metabolism in the blood, their eggs laying capacity and the incubation quality of the eggs, as well as the elimination of herbs and their preservation. The research was carried out during the 4-month reproductive period (December-March) on the gray Obroshino breed group of geese in the research enterprise (SE). The experimental farm (Myklashiv) of the Myklashiv Institute of Agriculture of the Carpathian region of NAAS (Myklashiv village, Pustomytiv district, Lviv region). For this purpose, 4 groups of geese-analogues by age and live weight were selected. The control group did not receive synthetic tryptophan supplements for feed additives. The level of this amino acid in this group amounted to 0.16 g per 100 g of mixed fodder. To the diet of the geese of 2-, 3-, 4-th experimental group additionally injected, respectively, 0.04; 0.09 and 0.14 grams of synthetic tryptophan per 100 g of mixed fodder. The conducted studies have shown that increasing the level of tryptophan in mixed fodders of geese with respect to the norms in force in Ukraine from 0.16 to 0.25 g due to its synthetic analogue per 100 g of mixed fodder during the oviposition period increases the hemoglobin level in the blood by 0.6 g %, white blood cells – by 10.2%, ammonium nitrogen – by 10.5%, stimulates fertility and improves the quality of incubation eggs. While analyzing productivity indices of geese, it should be noted that they were the highest in the 3rd experimental group receiving an additive of 0.09 grams of synthetic tryptophan per 100 grams of mixed fodder. The isolation of the caterpillars was the highest in the 3rd group and amounted to 79.3%. An important indicator, as the egg mass was the largest in the 3rd experimental group and was 172.7 grams or 3.1 g more than in the 1st control geese group. The analysis, namely width and length, shows that the most optimal egg shape index was found in the 3rd experimental group (1.8% higher than in the control group). The thickness of the shell of eggs in the experimental and control groups did not change significantly. It has been experimentally proved that the optimization of tryptophan levels in the feed grain of the gray Obroshin genera of geese in the reproductive period has a significant effect on the hematological parameters and the incubation quality of the eggs.

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IMPROVEMENT OF THE REPRODUCTIVE QUALITIES OF CATTLE WITH THE PREVENTION-N-B-S BIOLOGICAL

REPORTS ◽

10.32014/2021.2518-1483.8 ◽

2021 ◽

Vol 335 (1) ◽

pp. 53-60

Author(s):

V.G. Semenov ◽

D.A. Baimukanov ◽

S.G. Kondruchina ◽

Kh.A. Aubakirov ◽

T.N. Ivanova ◽

...

Keyword(s):

Postpartum Period ◽

Reproductive Function ◽

The Body ◽

Control Group ◽

Trial Group ◽

Critical Periods ◽

Nonspecific Resistance ◽

Biological Preparation ◽

Gynecological Diseases ◽

Experimental Group

Productive insemination of cows against the background of heat detection or synchronization of the estrous cycle within a relatively short time after the previous calving is the main factor that determines the productivity and profitability of dairy cattle breeding. Many obstetric and gynecological diseases often occur in the first 2 months of lactation. Postpartum obstetric and gynecological diseases of cows impair their reproductive qualities, which leads to prolonged anestrus, decreased fertility, and depletion of the body. Inflammatory processes of the female reproductive organs are accompanied by enhanced stress. The research aims to prevent diseases of the postpartum period and realize the potential of the reproductive qualities of cows by activating the nonspecific resistance of the organism with the Prevention-N-B-S biological preparation. Based on the performed studies, a method for the prevention of obstetric-gynecological diseases of the postpartum period and an increase in the reproductive function of cows by increasing the nonspecific resistance of the body with the biological Prevention-N-B-S is proposed. The newly designed biological preparation was for the first time used in cows of the 2nd experimental group intramuscularly at a dose of 10 ml 35-30, 15-10, and 10-5 days before the expected calving time, and the animals of the 1st experimental group were injected with Dorogov's antiseptic stimulator of fraction 2 in combination with eleovitum in a ratio of 1:9 60 days before the scheduled calving dates. It was found that in cows of the 2nd trial group (28.8±0.56 days), the first estrus began 5.8 and 14.4 days earlier than in the mates of the 1st trial group (34.6±0.93 days) and control (43.2±1.64 days) groups. There was a decrease in the conception rate in cows of the 1st (1.8±0.24) and 2nd (1.4±0.36) trial groups, in comparison with the control (2.6±0.26). The service period in the 1st (64.6±1.62 days) and 2nd (57.8±1.50 days) experimental groups decreased compared to the control group (89.2±3.02 days). In the first sexual estrus in the control, 1st and 2nd experimental groups, 2, 4, and 6 cows have become fertile, respectively. The use of biological in the critical periods of cow pregnancy reduced the risks of uterine subinvolution, endometritis, and mastitis in the postpartum period, and also increased the reproductive qualities of cows. The most expressed corresponding effect was obtained when using the biological preparation Prevention-N-B-S.

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ВПЛИВ МІКРОЕЛЕМЕНТІВ ТА ГУМІЛІДУ НА ВІДТВОРНУ ФУНКЦІЮ ТЕЛИЦЬ УКРАЇНСЬКОЇ М’ЯСНОЇ ПОРОДИ

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet6610 ◽

2016 ◽

Vol 18 (2(66)) ◽

pp. 44-48

Author(s):

V. Gryban ◽

D. Mylostiva ◽

E. Pecheniy

Keyword(s):

Trace Elements ◽

Physiological State ◽

Reproductive Function ◽

Protein Composition ◽

Biologically Active ◽

Control Group ◽

Group I ◽

Group Ii ◽

The Impact ◽

Experimental Group

The article presents research data on the impact of deficient dietary microelements (cobalt and selenium) and biologically active supplement Humilid on the indicators of the reproductive function of heifers after the first calving. We have found out that Humilid and trace elements influence on the physiological state of reproductive organs and stimulate the sexual heat of animals. Regarding the duration of the period from calving to fertilized insemination, the difference between the control and experimental groups is more visible. Thus, the service period in the experimental group I (under the influence of Humilid) was 293.2 days, which is 2.6 days less than in the control group. In the experimental group II (under the influence of cobalt and selenium), it was, respectively, by 3.9 days less than in the control one. This may indicate that the minerals and dietary supplements contribute to more rapid recovery of the ovarian cycle. Protein composition of blood serum was investigated in the study of the biochemical spectrum of blood. Balancing the nutrition of animals with deficient dietary microelements and adding Humilid increase the concentration of protein molecules, which is visible from the indicator of crude protein and its components - albumen and globulin.In comparison with animals in the control group, a total protein level of heifers in the experimental group I was higher by 8.8% (P <0.05), and in the experimental group II - by 8.6% (P <0.05). This may indicate the increased intensity of synthetic and redox processes in heifers’ bodies in preovulatory period which serve best for the insemination.The level of albumen was also higher by 8.0 and 7.3% respectively under the influence of Humilid and trace elements in relation to the control group, indicating the improvement of transport of substances, in our case estradiol.As far as globulin is concerned, the growth of this class of proteins was marked by 9.4 and 9.6%, indicating the improvement of the level of resistance of the organism. Thus, the increase in blood biochemical parameters creates certain conditions in heifers’ body to improve their insemination.

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The effects of a synthetic analogue of the Bovine Appeasing Pheromone on milk yield and composition in Valdostana dairy cows during the move from winter housing to confined lowland pastures

Journal of Dairy Research ◽

10.1017/s0022029918000262 ◽

2018 ◽

Vol 85 (2) ◽

pp. 174-177 ◽

Cited By ~ 8

Author(s):

Maria Cristina Osella ◽

Alessandro Cozzi ◽

Claudio Spegis ◽

Germano Turille ◽

Andrea Barmaz ◽

...

Keyword(s):

Dairy Cows ◽

Dry Matter ◽

Control Group ◽

Synthetic Analogue ◽

Skin Area ◽

Cow Performance ◽

Daily Milk Production ◽

And Control ◽

Experimental Group ◽

Daily Milk

This Research Communication describes the effects of a synthetic analogue of the Bovine Appeasing Pheromone (BAP) on milk parameters in Valdostana dairy cows during the first turning out from tie-stalls to confined lowland pastures around the farms. Thirty healthy lactating Valdostana cows were enroled in the study and randomly divided into 2 groups: experimental group (EG, n = 15) and control group (CG, n = 15). The two groups were separately housed in the same farm and managed outside in two different pens. Treatment (BAP and solution) and control (solution only) were poured on the nuchal skin area between the horns when the animals were inside the farm at the feeding rack every 7 d for 28 d (T0–T4). Milk samples were evaluated at the same time points (T0–T4). Daily milk production (kg/day) was higher in the EG than in the CG, particularly during the first day after the turning out to pasture (T1). Somatic Cell Count (103 cells/ml) was higher in the placebo group than in the EG, especially at T1. Proteins, fat, fat-free dry matter and casein (g/100 g) were not affected by the treatment. In T1 urea (mg/dl) content was higher in CG vs. EG, suggesting a more correct metabolic balance in the group treated with BAP. The use of BAP appears to modulate adaptation in ways that may improve dairy cow performance in the context of changes in management routines.

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Structural changes of mucous membrane and the presence of immunoglobulins in small intestines of neonatal pigs

Acta Veterinaria Brno ◽

10.2754/avb201382030317 ◽

2013 ◽

Vol 82 (3) ◽

pp. 317-322

Author(s):

Tomáš Kanka ◽

Michal Rolinec ◽

Danka Šťastná ◽

Ivan Imrich ◽

Daniel Bíro ◽

...

Keyword(s):

Mucous Membrane ◽

Structural Changes ◽

Postnatal Period ◽

Control Group ◽

Large White ◽

Neonatal Pigs ◽

Large White Breed ◽

White Breed ◽

Mucosal Changes ◽

Experimental Group

The structure of the small intestine of neonatal pigs has an important effect on immunoglobulin absorption. The aim of the experiment was to observe changes in the mucous membrane and the presence of immunoglobulins in the intestines of piglets during the first hours of their postnatal lives. In total, 80 piglets of the Large White breed were divided into two groups of 40 pigs. One group (control) came from an infection-free breeding, the other (experimental) group came from a breeding with the reproduction and respiration syndrome of pigs. Samples (n = 80) of duodenum, jejunum and ileum were taken at intervals of 0, 3, 6 and 12 h after colostrum intake in order to carry out histological tests. Primary pig antibodies were detected by immunohistochemical staining. The low content of immunoglobulins IgG, IgA and IgM was detected in all segments of intestines of precolostral piglets. Difference (P < 0.05) in size of the mucous membrane was found in the duodenum at the interval of 0, 3 and 6 h (46.03%), in the jejunum at 0 and 12 h (45.98%) and in favour of the control group. Compared to the control group, more Ig (P < 0.01) antibodies were observed in the experimental group: in the duodenum IgG at 12 h (37.22 vs. 10.81%), IgA, 6 h (43.29 vs. 16.71%), IgM, 12 h (25.29 vs. 8.40%), in the jejunum at IgG 12 h (23.97 vs. 9.96%), IgA, 0 h (0.48 vs. 0.37%) and IgM, 3 h (21.80 vs. 10.10%), in the ileum IgG, 0 h (0.38 vs. 0.29%), IgA, 3 h (20.46 vs. 6.45%), IgM, 3 h (31.5 vs. 12.29%). We can conclude that the colostrum intake and the presence of reproduction and respiration syndrome of pigs resulted in changes of microscopic structures of intestines as well as in the presence of Ig in piglet intestines. These mucosal changes significantly affect the development of passive immunity in piglets in the postnatal period.

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