scholarly journals COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

2016 ◽  
Vol 21 (2) ◽  
pp. 347-354 ◽  
Author(s):  
Néstor Rosales Loaiza ◽  
Patricia Vera ◽  
Cateryna Aiello-Mazzarri ◽  
Ever Morales
Keyword(s):  
Biochemical Composition ◽  
Protein Production ◽  
Nitrogen Concentration ◽  
Nitrogen Deficiency ◽  
Dry Mass ◽  
Nutritional Profile ◽  
Maximum Value ◽  
Essential Parameter ◽  
Anabaena Sp ◽  
High Concentrations

<p>Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with agricultural purposes. Growth and biochemical composition of Nostoc LAUN0015,Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 was compared at 0, 4.25, 8.5 and 17 mM NaNO3. Cultures under laboratory conditions were maintained for 30 days at a volume of 500 mL. Anabaenasp.1 yielded the highest value of dry mass of 0.26 ± 2.49 mg mL-1 at 8.5 mM NaNO3. For chlorophyll, phycocyanin and phycoerythrin were achieved maximum values at 17 mM NaNO3 with 18.09 ± 1.74, 102.90 ± 6.73 and 53.47 ± 2.40 μg mL-1, respectively. Nostoc LAUN0015 produced its maximum value of protein 644.86 ± 19.77 μg mL-1, and 890 mg mL-1 of carbohydrates in the absence of nitrogen. This comparative study shows that the most efficient strain for the production of protein, carbohydrates and lipids in diazotrophic conditions corresponded to Nostoc LAUN0015. However, Anabaena sp.1 and Anabaena sp.2 required high concentrations of nitrogen to achieve higher values of metabolites, comparing with Nostoc strains. Nitrogen dependence for the production of pigments and high protein production in strains of Anabaena and in diazotrophic conditions for Nostoc was demonstrated. Nostoc can be cultured under nitrogen deficiency andAnabaena in sufficiency, for mass production of biomass with good nutritional profile.</p>

scholarly journals Silicon fertigation and salicylic acid foliar spraying mitigate ammonium deficiency and toxicity in Eucalyptus spp. clonal seedlings

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250436
Author(s):  
Jonas Pereira de Souza Junior ◽  
Renato de Mello Prado ◽  
Thaís chagas Barros de Morais ◽  
Joaquim José Frazão ◽  
Marcilene Machado dos Santos Sarah ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Photosystem Ii ◽  
Nutrient Solution ◽  
Mass Production ◽  
Foliar Application ◽  
Nitrogen Deficiency ◽  
Photosynthetic Apparatus ◽  
Eucalyptus Grandis ◽  
Dry Mass ◽  
High Concentrations

Nitrogen deficiency and toxicity, primarily in its ammonium form (NH4+), can suppress plant growth and development. The use of silicon (Si) or salicylic acid (SA) may be an alternative to minimize the harmful effects of nutrient imbalances caused by NH4+, thereby improving the photosynthetic efficiency of plants. The aim of the present study was to assess the action of fertigation-applied Si and SA foliar spraying in mitigating NH4+ toxicity and deficiency in eucalyptus clonal seedlings. Two experiments were performed with eucalyptus clonal seedlings (Eucalyptus urophylla x Eucalyptus grandis), in a greenhouse. Both were carried out using a 4x2 factorial design and four concentrations of NH4+ (5, 15, 30 and 60 mmol L-1), in the absence and presence of Si (2 mmol L-1), in experiment I; or with and without SA foliar application (10−2 mmol L-1), in experiment II, with six repetitions. Nitrogen content rose as a result of increasing N-NH4+ concentration in the nutrient solution, and Si supplied via the nutrient solution was efficient in increasing the Si content in eucalyptus seedlings. The rise in N-NH4+ concentration favored the maintenance of the photosynthetic apparatus, but high N-NH4+ concentration increased energy loss through fluorescence and decreased the efficiency of photosystem II. The addition of Si to the nutrient solution proved to be beneficial to the photosynthetic apparatus by decreasing F0 at 15 and 30 mmol L-1 of NH4+; and Fm at all NH4+ concentrations studied. In addition, the beneficial element also increases Fv/Fm at all NH4+ concentrations studied. SA foliar application was also efficient in reducing photosynthetic energy losses by decreasing F0 and Fm at all NH4+ concentrations studied. However, SA only increased the Fv/Fm at the high concentrations studied (30 and 60 mmol L-1 of NH4+). Nitrogen disorder by deficiency or N-NH4+ toxicity reduced shoot dry mass production. The addition of Si to the nutrient solution and SA foliar application increased shoot dry mass production at all N-NH4+ concentrations studied, and benefitted the photosynthetic apparatus by decreasing fluorescence and improving the quantum efficiency of photosystem II as well as dry mass production.

scholarly journals EUTROPHICATION IN THE LOWER COASTAL BASIN OF THE JAMAPA RIVER IN VERACRUZ, MEXICO

2020 ◽  
Vol 5 (12) ◽  
pp. 206-216
Author(s):  
María del Refugio Castañeda-Chávez ◽  
Cinthya A Sosa-Villalobos ◽  
Isabel A Amaro-Espejo ◽  
Itzel Galaviz-Villa ◽  
Fabiola Lango-Reynoso
Keyword(s):  
Dissolved Oxygen ◽  
Nitrogen Concentration ◽  
Maximum Temperature ◽  
Mean Values ◽  
Maximum Value ◽  
Statistical Program ◽  
Coastal Basins ◽  
High Concentrations ◽  
Reduced Forms ◽  
Coastal Basin

The aim of this study was to determine parameters of dissolved oxygen, temperature and nitrogen in the coastal basin of the Jamapa River as indicators of eutrophication. Six sampling sites were selected in the lower basin, where samples were collected in triplicate in each site, during three seasons; north winds, dry and rainy. Results of the research, analyzed using the Minitab statistical program, were: site 2 showing the maximum value of dissolved oxygen with 5.83 mg/L; site 5 with the maximum temperature value of 30.3 ºC, during the dry season. Whereas, maximum mean values of total nitrogen were at site 2 (4.596 mg/L) and site 5 (4.281 mg/L). Coastal basins can change markedly the nitrogen concentration with respect to weather and environmental conditions. As well as, reduced forms of nitrogen cause alterations in dissolved oxygen and the increase in water temperature, along with high concentrations of nutrients.

scholarly journals Potential of macrophyte for removing arsenic from aqueous solution

2012 ◽  
Vol 30 (4) ◽  
pp. 683-696 ◽  
Author(s):  
F.P. Guimaraes ◽  
R. Aguiar ◽  
J.A. Oliveira ◽  
J.A.A. Silva ◽  
D. Karam
Keyword(s):  
Aquatic Macrophytes ◽  
Leaf Size ◽  
Mass Gain ◽  
Dry Mass ◽  
Phosphate Deficiency ◽  
Lemna Gibba ◽  
High Concentration ◽  
Carotenoid Synthesis ◽  
High Concentrations ◽  
Selection Of

The potential of three aquatic macrophytes, Azoll caroliniana, Salvinia minima and Lemna gibba, was evaluated in this work aimed at selection of plants to be used in remediation of environments contaminated by arsenic (As). The experiments were carried out in a greenhouse during six days in pots containing Hoagland solution (¼ ionic strength) at As concentrations of 0.5; 2.5 and 5.0 mg L-1. The three species showed greater As accumulation as the concentration of the metalloid in solution increased. However, a reduction was detected in fresh and dry mass gain when the plants were exposed to high As concentrations. The macrophytes showed differences in efficiency of removal of As in solution. A. caroliniana, S. minima and L. gibba accumulated, on average, 0.130; 0.200; and 1.397 mg mDM-1, respectively, when exposed to 5.0 mg L-1 of As. The macrophytes absorbed a greater quantity of As in solution with low phosphate content. The greater As concentration in L. gibba tissues lowered the chlorophyll and carotenoid contents as shown by the high chlorosis incidence. Lemna gibba also exhibited a decrease in leaf size, with the total chlorophyll and carotenoid synthesis not being affected by As in A. caroliniana. This species exhibited purplish leaves with high concentration of anthocyanin, whose presence suggested association to phosphate deficiency. Marginal necrosis occurred on S. minima floating leaves, with the released daughter-plants not showing any visual symptoms during the treatment. The percentage of As removed from the solution decreased when the plants were exposed to high concentrations of the pollutant. Among the three species studied, only L. gibba could be considered an As hyper-accumulator. The use of this plant species for remediation of aquatic environments was shown to be limited and requires further investigation.

Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus

2005 ◽  
Vol 17 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Natalia O. Zhila ◽  
Galina S. Kalacheva ◽  
Tatiana G. Volova
Keyword(s):  
Green Alga ◽  
Biochemical Composition ◽  
Nitrogen Deficiency

scholarly journals Mycelial biomass cultivation of Lentinus crinitus

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Itaruã Machri Colla ◽  
Olavo Bilac Quaresma de Oliveira Filho ◽  
Janyeli Dorini Silva de Freitas ◽  
Míria Benetati Delgado Bertéli ◽  
Giani Andrea Linde ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Nitrogen Concentration ◽  
Fungal Growth ◽  
Malt Extract ◽  
Biomass Growth ◽  
Mycelial Biomass ◽  
Protein Nitrogen ◽  
Temperature Growth ◽  
Nitrogen Concentrations ◽  
High Concentrations

Lentinus crinitus is a medicinal basidiomycete, little studied regarding the basic cultivation conditions, which is used in bioremediation and consumed by native Indians from the Brazilian Amazon. Also, it produces a fungal secondary metabolite panepoxydone that has been described as an essential regulator of the inflammatory and immune response. This study aimed to evaluate basic conditions of temperature, pH, and nitrogen concentration and source in the cultivation of L. crinitus mycelial biomass. In order to evaluate fungal growth temperature, 2% malt extract agar (MEA) medium, pH 5.5, was utilized from 19 to 40 °C. For pH, MEA had pH adjusted from 2 to 11 and cultivated at 28 °C. Urea or soybean meal was added to MEA to obtain final concentration from 0.5 and 16 g/L of nitrogen, pH of 5.5, cultivated at 28 °C. The best temperature growth varies from 31 to 34 ºC and the optimal one is 32.7º C, and the best pH ranges from 4.5 to 6.5 and the optimal one is 6.1. Protein or non-protein nitrogen concentration is inversely proportional to the mycelial biomass growth. Nitrogen concentrations of 2.0 g/L soybean meal and urea inhibit mycelial biomass growth in 11% and 12%, respectively, but high concentrations of 16.0 g/L nitrogen inhibit the growth in 46% and 95%, respectively. The fungus is robust and grows under extreme conditions of temperature and pH, but smaller adaptation with increasing nitrogen concentrations in the cultivation medium, mainly non-protein nitrogen.

scholarly journals Nutrient enrichment effect on macroinvertebrates in a lowland stream of Argentina

2015 ◽  
Vol 105 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Agustina Cortelezzi ◽  
Carolina Ocón ◽  
María V. López van Oosterom ◽  
Rosana Cepeda ◽  
Alberto Rodrigues Capítulo
Keyword(s):  
Climate Change ◽  
Global Climate Change ◽  
Global Climate ◽  
Nitrogen Concentration ◽  
Nutrient Concentrations ◽  
Phosphorus Concentration ◽  
Lowland Stream ◽  
Pampean Streams ◽  
High Concentrations ◽  
Intensive Land Use

ABSTRACT One of the most important effects derived from the intensive land use is the increase of nutrient concentration in the aquatic systems due to superficial drainage. Besides, the increment of precipitations in South America connected to the global climate change could intensify these anthropic impacts due to the changes in the runoff pattern and a greater discharge of water in the streams and rivers. The pampean streams are singular environments with high natural nutrient concentrations which could be increased even more if the predictions of global climate change for the area are met. In this context, the effect of experimental nutrient addition on macroinvertebrates in a lowland stream is studied. Samplings were carried out from March 2007 to February 2009 in two reaches (fertilized and unfertilized), upstream and downstream from the input of nutrients. The addition of nutrients caused an increase in the phosphorus concentration in the fertilized reach which was not observed for nitrogen concentration. From all macroinvertebrates studied only two taxa had significant differences in their abundance after fertilization: Corbicula fluminea and Ostracoda. Our results reveal that the disturbance caused by the increase of nutrients on the benthic community depends on basal nutrients concentration. The weak response of macroinvertebrates to fertilization in the pampean streams could be due to their tolerance to high concentrations of nutrients in relation to their evolutionary history in streams naturally enriched with nutrients. Further research concerning the thresholds of nutrients affecting macroinvertebrates and about the adaptive advantages of taxa in naturally eutrophic environments is still needed. This information will allow for a better understanding of the processes of nutrient cycling and for the construction of restoration measures in natural eutrophic ecosystems.

Anaerobic Degradation of Municipal Wastes in Landfill

1992 ◽  
Vol 25 (7) ◽  
pp. 243-253 ◽  
Author(s):  
A. Attal ◽  
J. Akunna ◽  
P. Camacho ◽  
P. Salmon ◽  
I. Paris
Keyword(s):  
Sampling Technique ◽  
Drill Hole ◽  
Dry Mass ◽  
General Information ◽  
Landfill Site ◽  
Transformation Rate ◽  
Acceptable Error ◽  
Maximum Value ◽  
Thermophilic Conditions ◽  
Methane Potential

The objective of this study was to analyze the degree of decomposition of municipal solid waste (MSW) placed in a landfill site and, based on this analysis, to propose an investigatory procedure to be used to assess the energetic potential of any site. A sampling technique was developed in order to reduce the size of the MSW sample to 25-30 kg with an acceptable error of 20 %. Profiles of the strata were established which have shown a well ordered structure in horizontal layers corresponding to different MSW ages. The location of the two drill holes were chosen consequently. A sample per MSW age was carried out for each drill hole. Their degradation states were measured using an accelerated biodegradability test which also gives the methane potential. For all samples, the evolution of four parameters, temperature, % CH4, % VSS and methane potential, were analysed as a function of their age. Anaerobic microbial activity was detected in the top layer of the landfill, showing that refuse starts as soon as deposited to undergo anaerobic transformation. Indeed, the % CH4 reaches its maximum value of 60 % between 5 and 10 m. Temperature increases from about 26°C to 50°C in the first 15 m and stays at this maximum value. That shows that municipal wastes are degraded under mesophilic and thermophilic conditions. Two relations between waste age and % VSS, and waste age and the energetic potential have been demonstrated. The % VSS represents about 65 % of the dry mass for the fresh wastes and is quite low for the 6 year-old refuse. This rapid waste transformation rate could be due to optimal conditions for anaerobic activity existing in the landfill. The methane potential is proportional to waste age, almost zero for the 6 year-old refuse and greater than 45 1.kg.−1 for fresh wastes. These results show that a simple and rapid measurement of the % VSS of an unknown sample could be a good indication of its age and its degradation state. As a result of this study, a two-step procedure has been developed that enables an evaluation of the energetic potential of a landfill site. The first step is to collect all the general information on the site, thus providing an initial estimate of the decomposition degree within the mass of the waste. The second step is then to confirm this estimation by sampling and analysing a few samples taken at shallow depths.

Nutrient homeostasis, metabolism of reserves, and seedling vigor as affected by seed priming in coarse rice

2006 ◽  
Vol 84 (8) ◽  
pp. 1196-1202 ◽  
Author(s):  
M. Farooq ◽  
S.M.A. Basra ◽  
M. Khalid ◽  
R. Tabassum ◽  
T. Mahmood
Keyword(s):  
Reducing Sugars ◽  
Amylase Activity ◽  
Nitrogen Concentration ◽  
Ion Homeostasis ◽  
Seed Priming ◽  
Dry Mass ◽  
Seedling Vigor ◽  
Control Seed ◽  
Positive Correlations ◽  
Secondary Roots

The influence of seed priming on germination, seedling vigor, ion homeostasis, and starch metabolism in coarse rice was studied. Priming treatments included pregermination (a traditional soaking method being used for rice nursery preparation), hydropriming for 48 h, osmohardening with KCl or CaCl2 (ψs –1.25 MPa solution) for 24 h, ascorbate priming (10 mg·L–1) for 48 h, and hardening for 24 h. Compared with controls, all priming treatments (except pregermination) reduced the time to start germination, improved the rate of germination and synchronization, and the length of shoot and root, seedling fresh and dry mass, number of secondary roots, the concentration of reducing sugars, and α-amylase activity, although the extent of these changes was different in seeds subjected to different treatments. These seed treatments resulted in higher germination that might be due to overcoming dormancy. Osmohardening with KCl was more effective than CaCl2 for these parameters. Nitrogen concentration remained unaffected in seedlings; however, Ca2+ concentrations in both seeds and seedlings were greater in seeds osmohardenerd with CaCl2 than with all other treatments, including the control. Seed priming enhanced K+ concentration in both seeds and seedlings, leading to improved α-amylase activity. There were positive correlations between seed K+ concentration and amylase activity, and the concentration of reducing sugars with amylase activity, seedling dry mass, or number of secondary roots. Osmohardening with KCl performed better than all other treatments including control. Priming improved the K+ balance that activates α-amylase, a basis for seed invigoration.

scholarly journals Isolation, Screening and Optimization of Estuary Region (Khambhat, Gujarat) Microalgae for Lipid/Oil Production

2015 ◽  
Vol 3 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Mahendraperumal Guruvaiah ◽  
Madhuri Narra ◽  
Garima Dixit ◽  
Punit Karawadia ◽  
Deval Shah
Keyword(s):  
Nitrogen Concentration ◽  
Dry Mass ◽  
Total Hardness ◽  
Acetyl Coa Carboxylase ◽  
Standard Laboratory ◽  
Solvent Extraction Method ◽  
Cyanobacterial Species ◽  
Water And Soil Samples ◽  
Unialgal Culture ◽  
Mass Basis

Water and soil samples were collected from gulf of Khambhat region, Gujarat. The cations and anions like calcium, chloride, fluoride, magnesium, sulphate and total hardness as calcium carbonate were analyzed. Collected estuarine samples were cultured in three different media under standard laboratory conditions. These enrichments were then used to isolate pure unialgal culture by conventional method. Thirty four isolates belonging to twelve species of Cyanobacteria, twenty species of Chlorophyta and two taxa of Bacillariophyta were identified and are maintained at SPRERI centre. The Chlorophyta were found better accumulators of lipids than the cyanobacterial species. Five promising strains (SBC 7, SBC 9, SBC 17, SBC 18 and SBC 19) have been selected. In-house isolates SBC19 and SBC 17 showed highest acetyl CoA carboxylase (ACCase) of 55.2 (U/ml) and 51.2 (U/ml) respectively, with 0.375 g l-1 nitrogen concentration in 24 days. Biomass production was highest for 2.4 g/l, (SBC 19) and 2.7 g/l (SBC 17) with 1.5 g l-1 nitrogen concentration. The highest lipid content was 52% and 48% in SBC19 and SBC 17, respectively, with 0.375 g l-1 nitrogen concentration of solvent extraction method. Lipid accumulation was found enhanced by more than 50% on dry mass basis under nitrogen starvation.Int J Appl Sci Biotechnol, Vol 3(2): 197-201 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12534 

scholarly journals Critical nitrogen dilution curves and productivity assessments for plant cane

2020 ◽  
Vol 24 (4) ◽  
pp. 244-251
Author(s):  
Augusto C. de A. Santana ◽  
Emídio C. A. de Oliveira ◽  
Vinícius S. G. da Silva ◽  
Renato L. dos Santos ◽  
Magda A. da Silva ◽  
...  
Keyword(s):  
Nitrogen Fertilization ◽  
Nitrogen Concentration ◽  
Dry Mass ◽  
Exponential Model ◽  
Critical Levels ◽  
Sugarcane Varieties ◽  
Nitrogen Dilution ◽  
Limiting Nutrient ◽  
Critical Nitrogen Concentration ◽  
Stalk Yield

ABSTRACT Nitrogen is the most limiting nutrient for the development of sugarcane and particularly the use of more productive and demanding varieties, for which the doses of nitrogen fertilization necessary during the plant cane cycle may be underestimated and the critical levels of nitrogen in the leaf may be modified. The objective of this study was to determine the critical nitrogen dilution curve (CNDC) for leaves, evaluate the aboveground dry mass production (DM) and productivity of two varieties fertilized with N in the plant cane cycle. The study was conducted under field conditions, evaluating two sugarcane varieties RB92579 and RB867515, fertilized with seven doses of N (0, 30, 60, 90, 120, 150 and 200 kg ha-1). The varieties and doses were arranged in randomized blocks and analyzed in the 2 × 7 factorial scheme. It was found that the nitrogen concentration in the leaf decreased with four repetitions increase in DM yield and adjusted an exponential model, which enabled to estimate the critical levels of 13.03 and 12.46 g N kg-1 dry matter in the varieties RB92579 and RB867515, respectively. The plant cane cycle were observed to respond positively to nitrogen fertilization, with increase of 18.66% in tons of stalk per hectare (TSH) in response to the application addition of up to 129.45 kg N ha-1. The variety RB92579 showed the lowest dilution of the critical nitrogen concentration, the highest production of DM and higher stalk yield, and accordingly is considered to be the more efficient of the two varieties with respect to nitrogen fertilizer utilization.

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