scholarly journals Antioxidant activity of two varieties of Ocimum basilicum L. for potential use in phytocosmetics

2016 ◽  
Vol 69 (2) ◽  
pp. 7965-7973 ◽  
Author(s):  
Andrea Maritza Vivas Castaño ◽  
Martha Cecilia Beltrán Cifuentes ◽  
Deisy Johanna Cañón Rincón
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Ocimum Basilicum ◽  
Raw Material ◽  
Ferric Thiocyanate ◽  
Type Device ◽  
Cosmetic Industry ◽  
Industrial Use ◽  
Potential Use

This investigation aimed to evaluate two varieties of Ocimum basilicum L., known as Basil, as potential raw material for the cosmetic industry, assessing their antioxidant properties, considering their industrial use in phytocosmetics. The antioxidant activity of essential oils (EOs) for the species Ocimum basilicum var. cinammom and var. album, were obtained by distillation steam using a Clevenger-type device. The antioxidant capacity was evaluated by the method of bleaching radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) and the method of linoleic acid peroxidation (ferric thiocyanate). The EOs of the two species had significant antioxidant properties. The method of DPPH facilitated the evaluation of the antioxidant capacity versus the concentration of EOs, showing an efficient concentration at 10 ppm. On the other hand, the ferric thiocyanate method displayed an efficient inhibition up to 360 h (15 d). The obtained results demonstrated the antioxidant capacity of EOs in the investigation. The capacity was related to their chemical composition (phenylpropane and oxygenated monoterpenes). Therefore, EOs can be considered as a potential source in the field of phytocosmetics.

scholarly journals Original Research Article Investigation of Antioxidant Potential in Ocimum basilicum Flower

2021 ◽  
pp. 56-64
Author(s):  
Sadaf Khurshid Kayani
Keyword(s):  
Antioxidant Capacity ◽  
Column Chromatography ◽  
Antioxidant Properties ◽  
Ocimum Basilicum ◽  
Original Research ◽  
Thiocyanate Complex ◽  
Dark Bottle ◽  
Phenolic Contents ◽  
Ferric Thiocyanate

Aims: The present work is particularly focused on antioxidant properties of flower of Ocimum basilicum plant. Study Design: Study is basically designed on Column chromatography of extracts. Place and Duration of Study: Sample collection and all experimental work was done in Chemistry Department Government College University, Lahore. The study comprises duration of 6 months. Methodology: The flower of Ocimum basilicum were collected, dried and grinded. It was soaked in methanol-water (70:30) in dark bottle for a week. Followed by a scheme (column chromatography). After TLC of extracts, three activities were done. Phosphomolybdate, Ferric thiocyanate (FTC), and Folin-Ciocalteu (FC reagent) for determination of antioxidant capacity, peroxidation, determination of total phenols respectively. Results: The sample OC2 and crude have maximum absorbance at the concentration of 100µl, 200µl and 300µl. The results show that crude has maximum antioxidant capacity. The phenolic contents are in the increasing order of fraction OC2, OC5, and crude. The maximum phenolic contents are present in crude. Reference has the maximum ability for peroxidation for ferric thiocyanate complex by giving red colour. Conclusion: Overall it is concluded that Ocimum basilicum flower has antioxidant capacity as good as a standard antioxidant. It is recommended in food/medicine as natural herbal product.

scholarly journals Antioxidant properties, quantification and stability of betalains from pitaya (Hylocereus undatus) peel

2014 ◽  
Vol 45 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Fernanda Robert de Mello ◽  
Claudia Bernardo ◽  
Caroline Odebrecht Dias ◽  
Luciano Gonzaga ◽  
Edna Regina Amante ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Raw Material ◽  
High Concentration ◽  
Natural Colorant ◽  
Wide Ph Range ◽  
Hylocereus Undatus ◽  
Ph Range ◽  
Promising Source

Pitaya peel can be used as a raw material for betalains extraction. The aim of this research was to quantify phenolic compounds, antioxidant activity and betalains on pitaya peel. Furthermore, evaluate the betalains stability against various pH conditions and exposure time of heating. The results showed that pitaya peel contains phenolic compounds and presented antioxidant activity. Moreover it showed high concentration of betalains (101.04mg equivalent to betanin. 100g-1) which were stable over a wide pH range (3.2 - 7.0) and were resistant to heating (100oC) up to 10 minutes at pH range from 3.7 to 5.5. Therefore, pitaya peel is a promising source of betalains which can be applied as a natural colorant for food.

scholarly journals The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana) and its antioxidant activity

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Fily Larasati ◽  
IRMANIDA BATUBARA ◽  
YULIN LESTARI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Rrna Gene ◽  
Blue Color ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Endophytic Actinobacteria

Abstract. Larasati F, Batubara I, Lestari Y. 2020. The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana L.) and its antioxidant activity. Biodiversitas 21: 1488-1497. Mangosteen (Garcinia mangostana L.) is a family member of Clusiaceae which is rich in secondary metabolite compounds that can function as antioxidants. Besides being produced by its host plant, the bioactive compounds can also be produced by endophytic actinobacteria. The purpose of this study was to explore the presence of endophytic actinobacteria from mangosteen peel and determine its antioxidant activity. The actinobacteria were isolated, purified, morphologically characterized, molecularly identified, extracted with ethyl acetate and tested for antioxidant properties. The antioxidant activity was assayed using DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) methods. The components of extracts were separated by Thin Layer Chromatography (TLC) and bioautography was done to determine the antioxidant bands. As a result, five isolates of endophytic actinobacteria in mangosteen peel showed to have difference in aerial mycelium color, substrate mycelium color, and types of spore chains. Based on 16S rRNA gene analysis, AGM3.2 isolate showed similarity with Streptomyces griseochromogenes ATCC 14511 (T) 99.06%. AGM3.1 had similarity with Streptomyces osmaniensis OU-63 (T) 98.35%. Meanwhile, AGM2.3 were similar to Streptomyces xanthophaeus NBRC B-5414 (T) 99.82%, AGM2.2 had similarity with Streptomyces xanthophaeus NBRC B-5414 (T) 98.95%. In addition, AGM2.1 has homology with Streptomyces goshikiensis NBRC 12868 (T) 99.52%. Using both DPPH and ABTS, supernatant of AGM2.1 showed the highest antioxidant activity indicated by 36.96 and 98.80 inhibition, respectively. Antioxidant capacity of ethyl acetate extract of AGM2.1 was 22.22 μg AEAC/mg extract (DPPH) and 20.34 μg AEAC/mg extract (ABTS). Meanwhile, ethyl acetate extract of mangosteen peel had antioxidant capacity by 21.17 µg AEAC/mg extract (DPPH) and 18.75 µg AEAC/mg extract (ABTS). Antioxidant bioautographic analysis of mangosteen peel ethyl acetate extract was compared with alpha mangosteen standard. The results showed that alpha mangosteen presence in the mangosteen extract with the same Rf value of 0.64 with standard. Meanwhile, actinobacterial ethyl acetate extract from AGM3.1, AGM2.3, AGM2.2, AGM2.1 each have the same Rf value with the alpha mangosteen standard. However, the spot for alpha mangosteen had dark red color, while spots of the four actinobacterial isolates showed to have blue color indicating different antioxidant compounds. The blue spot indicates the flavone, flavanone, flavonol, and isoflavone. These compounds include a subgroup of flavonoid compounds. Ethyl acetate extract AGM3.2 does not have spot compounds with the same Rf value as the alpha mangosteen standard. Study clearly shows that endophytic actinobacteria from mangosteen peel have potency as antioxidant.

scholarly journals Prospective use of fruit byproducts in Colombia according to their antioxidant capacity

2020 ◽  
Vol 44 (173) ◽  
pp. 1113-1125
Author(s):  
Ciliana Flórez-Montes ◽  
Óscar Marino Mosquera-Martínez ◽  
Andrés Felipe Rojas-González
Keyword(s):  
Antioxidant Capacity ◽  
Reducing Power ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Cosmetic Industry ◽  
Fruit Processing ◽  
Ferric Reducing Antioxidant Power ◽  
Valuable Compounds ◽  
Potential Use ◽  
Tree Tomato

Currently, the use of agro-industrial waste represents a viable alternative for obtaining valuable compounds that, in the case of biorefineries, is an opportunity for improvement. In this context, the aim of our study was to determine the flavonoid content and the antioxidant capacity of 30 byproducts from fruit processing in Colombia and propose possible applications. We analyzed the antioxidant capacity of the ethanolic extracts from each byproduct using the ferric reducing antioxidant power (FRAP) and the reducing power of antioxidant activity (RPAA) assays, as well as the oxygen radical absorbance capacity (ORAC) test. We found that tree tomato peels and stem and peach peels had a high flavonoid content, with values greater than 8,271.82 ± 702.70 μg quercetin per gram of dry sample. We also found that mango (22,676.57 ± 759.71 μg TE1/g Sdb, 3,692.38 ± 92.67 μg GAE/g Sdb), soursop (22,117.13 ± 754.94 μg TE1/g Sdb, 4,858.79 ± 156.71 μg GAE/g Sdb, 14,713.39 ± 757.95 μg TE2/g Sdb), grape (17,027.85 ± 765.11 μg TE1/g Sdb, 13,395.15 ± 659.31 μg TE2/g Sdb), peach peels (17,910.21 ± 1,424.33 μg TE2/g Sdb) and seeds (4,316.46 ± 112.00 μg GAE/g Sdb, 20,093.32 ± 1,317.93 μg TE2/g Sdb), and grape stalk (3,552.26 ± 31.63 μg GAE/g Sdb) showed a high antioxidant capacity in the different tests performed. Our results demonstrate that fruit byproducts have potential use in the pharmaceutical,  ood, and cosmetic industry due to  heir flavonoids content and their high antioxidant capacity.

scholarly journals Phytochemical Diversity in Rhizomes of Three Reynoutria Species and their Antioxidant Activity Correlations Elucidated by LC-ESI-MS/MS Analysis

2019 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sylwester Ślusarczyk ◽  
Sebastian Granica ◽  
Jakub Hadzik ◽  
Adam Matkowski
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Therapeutic Effects ◽  
Similar Species ◽  
Reynoutria Japonica ◽  
Esi Ms ◽  
Total Antioxidant ◽  
First Time

One of the richest natural sources of resveratrol - the rhizome of Reynoutria japonica in East Asia is a well-known traditional herb (Hu zhang) used in various inflammatory diseases, infections, skin diseases, scald, hyperlipidemia. Although, it has been recently included in the European Pharmacopoeia, still in Europe is an untapped resource. Some of the therapeutic effects are likely to be influenced by its antioxidant properties and this in turn is frequently associated with a high stilbene content. However, some literatures suggested that other compounds than stilbenes may add to the total antioxidant capacity. Hence, the aim of this research was to examine rhizomes of R. japonica and less studied, morphologically similar species, R. sachalinensis and R. x bohemica for their phytochemical composition and antioxidant activity and to clarify the relationship between the antioxidant activity and compounds by statistical methods. HPLC/UV/ESI-MS studies of three Reynoutria species revealed 171 compounds comprising stilbenes, carbohydrates, procyanidins, flavan-3-ols, anthraquinones, phenylpropanoids, lignin oligomers, hydroxycinnamic acids, naphthalenes and their derivatives. Our studies confirmed the presence of procyanidins with high degree of polymerization, up to decamers in the rhizomes of R. japonica and brings new data on the presence of these compounds in other Reynoutria species. A procyanidin trimer digallate was described for the first time in the studied plants. Moreover, we suggested a presence of new for these species, dianthrone glycosides and previously unrecorded phenylpropanoid disaccharide esters and hydroxycinnamic acid derivatives, mainly in R. sachalinensis. Furthemore, compounds tentatively annotated as lignin oligomers were observed for the first time in studied species. The rhizomes of all Reynoutria species exhibited strong antioxidant activity. Statistical analysis demonstrated that proanthocyanidins should be considered as important contributors to the total antioxidant capacity.

scholarly journals Antioxidant Activity and Combination Characteristics of Filtrates and Sargassum polycystum Seaweed Salt Residue

2021 ◽  
Author(s):  
Nurjanah Nurjanah ◽  
◽  
Asadatun Abdullah ◽  
Amalia Rahmadhani ◽  
Anggrei Viona Seulalae ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Dietary Fiber ◽  
Brown Seaweed ◽  
Fiber Content ◽  
Raw Material ◽  
National Standard ◽  
High Yield ◽  
Dietary Salt ◽  
Sargassum Polycystum

Sargassum polycystum is a low-sodium salt raw material that met the dietary salt criteria, i.e., low Na: K ratio and NaCl content < 60%. This study aims to produce seaweed salt with high yield, dietary fiber content, and antioxidant activity according to the quality standard of dietary salt through a combination of the filtrate and salt extraction residue. The results showed that the produced S. polycystum brown seaweed salt had a mineral Na 96.97 mg/g and mineral K 247.59 mg/g. Na: K ratio of 0.39, NaCl content of 49.05%, and heavy metal residue below the Indonesian National Standard maximum standard for dietary salt. The levels of dietary fiber produced in salt samples 1:1, 1:2, and 2:1 were 34.41%, 40.16%, and 23.83%, respectively, and the iodine content (KIO3) of the control salt was 125.95 mg/kg. The antioxidant activity of seaweed salt 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) on salt K (seaweed salt without residue), residue, S1 (1:1), S2 (1:2) and S3 (2:1) was 26.41 ppm, 59.06 ppm, 12.29 ppm, 67.30 ppm and 87.50 ppm. Meanwhile, the antioxidant capacity of seaweed salt Cupric Reducing Antioxidant Capacity (CUPRAC) on K salt, residue, S1, S2 and S3 were 12.36, 19, 7.86, 8.52 and 12.27 μmol ascorbic acid/g extract, respectively. Therefore, it was concluded that increasing the yield and dietary fiber content in the seaweed salt production process is achievable by adding residues.

scholarly journals Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
RIDWANTI BATUBARA ◽  
TENGKU ISMANELLY HANUM ◽  
ODING AFFANDI ◽  
HENNY SRI WAHYUNI
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Chemical Compounds ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Mature Leaves ◽  
Dpph Method ◽  
North Sumatra ◽  
Chemical Contents

Abstract. Batubara R, Hanum TI, Affandi O, Wahyuni HS. 2020. Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties. Biodiversitas 21: 4616-4622. The genus Wikstroemia is one of the genus of agarwood producing trees which has not been widely researched and published. One type of this genus is Wikstroemia tenuiramis Miq, which grows naturally in North Sumatra, the leaves are like other types of agarwood leaves can be used as raw material for agarwood leaves tea. A research has been conducted to determine the chemical contents contained in the young and mature leaves of agarwood plants species W. Tenuiramis. This research methods applied qualitative analysis using phytochemical screening, quantitative analysis to determine tannin content using titration method, antioxidant activity analysis using 2,2-diphenil-1-picrylhydrazil (DPPH) method, and compound tracing using Py-gas chromatography-mass spectrometry (Py-GC-MS) instruments. The qualitative phytochemical analysis results showed no difference in chemical compounds contained in both the young and mature leaves. Both the young and mature leaves contained flavonoids, triterpenoids, and tannins, and did not contain alkaloids, steroids and saponins. The young contained tannins of 1.079 ± 0.001% and the mature contained tannins of 4.645 ± 0.021%, not significant statistically. Py-GC-MS analysis showed the presence of various 30 bioactive compounds contained in both young and mature leaves. The analysis results also showed that both the young and mature leaves had very strong antioxidant activity. Our findings suggest that the two types of leaves have equal utility value of in term of chemical contents and antioxidant potentials.

Influence of thermal modification and extraction techniques on yield, antioxidant capacity and phytochemical profile of chestnut (Castanea sativa Mill.) wood

Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Immacolata Faraone ◽  
Daniela Russo ◽  
Maurizio D’Auria ◽  
Maria Roberta Bruno ◽  
Paola Cetera ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Castanea Sativa ◽  
Total Content ◽  
Thermal Modification ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Compounds ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction

AbstractNumerous investigations on the antioxidant properties of different plant tissue extracts are available in literature, but few studies on the effect of thermally modified wood on secondary metabolites were carried out up to now. This study highlights the influence of the thermal modification of Castanea sativa Mill. wood on total content of antioxidant compounds and consequently on its antioxidant activity. In addition, a chemical profile by Gas Chromatography–Mass Spectrometry (GC–MS) of the extractives was carried out. Wood of chestnut, thermally modified at 180 °C for 3 h, was used to obtain wood meal which was subjected to different extraction techniques, as maceration extraction (ME), ultrasound assisted extraction (UAE) and accelerated solvent extraction (ASE). The total content of principal antioxidant compounds, such as polyphenols, flavonoids and tannins, as well as the evaluation of antioxidant capacity by using different in vitro assays were determined. Relative Antioxidant Capacity Index (RACI), which is used to compare all antioxidant parameters, has also been applied. The study demonstrated a positive influence on chemical compounds present in C. sativa Mill wood originating from the thermal modification process. Thus, is possible to consider thermal modification as a promising strategy to improve the antioxidant activity of chestnut wood extractives.

scholarly journals Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 992 ◽  
Author(s):  
Joanna Pokorska ◽  
Dominika Kułaj ◽  
Agata Piestrzyńska-Kajtoch ◽  
Anna Radko
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Lipocalin 2 ◽  
Holstein Friesian ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Daily Milk Yield ◽  
Friesian Cows

In the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.

scholarly journals Polyphenols, L-Ascorbic Acid, and Antioxidant Activity in Wines from Rose Fruits (Rosa rugosa)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2561
Author(s):  
Andrzej Cendrowski ◽  
Marcin Królak ◽  
Stanisław Kalisz
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Quality Characteristics ◽  
Raw Material ◽  
Rosa Rugosa ◽  
Fruit Wine ◽  
High Antioxidant Activity ◽  
The Stability

The aim of the present study was to determine the influence of the winemaking process on the antioxidant potential and content of phenolic compounds and L-ascorbic acid in wines from the fruits of Rosa rugosa. The results obtained in this study clearly indicate that the fruits of the Rosa rugosa are a desirable raw material for the production of fruit wine. The parameters of the technological process of producing wines from rose fruits had a diversified influence on the tested quality characteristics. Aged wines contained phenolics levels of 473–958 mg/100 mL GAE. The final concentrations of ascorbic acid ranged from 61 to 155 mg/100 mL for the different variants of the wine. Wines revealed high antioxidant activity in assay with DPPH. On the basis of the obtained results, it can be assumed that all the applied variants of the winemaking process are suitable for rose fruit wine. Each variant ensured at least the stability of the antioxidant capacity.

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