scholarly journals Antifungal effect from Zingiber officinale, Aloe vera and Trichoderma sp. for control of Moniliophthora roreri in Theobroma cacao in Huánuco, Peru

2022 ◽  
Vol 75 (1) ◽  
Author(s):  
Rocio Reyna Soto Chochocca ◽  
Elena Gonzales Avila ◽  
Joel Hugo Fernandez Rojas ◽  
Julio Miguel Angeles Suazo ◽  
Alex Rubén Huamán De La Cruz ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Aloe Vera ◽  
Zingiber Officinale ◽  
Inhibitory Effect ◽  
Fruit Weight ◽  
Cultural Control ◽  
Raw Material ◽  
Efficient Management ◽  
Pharmaceutical Industries ◽  
Moniliophthora Roreri

Theobroma cacao is the main raw material to produce chocolate, as well as for use in the food, cosmetic, and pharmaceutical industries. However, Moniliophthora roreri is one of the most destructive fungal diseases and the main limiting of cacao production worldwide. Thus, this work aimed to assess the inhibitory effect of extracts of Zingiber officinale (T1) and Aloe vera (T2), and Trichoderma harzianum + Bacillus subtillis. (T3) on Moniliophthora roreri infection in Theobroma cacao; in addition, a control (T4) was also evaluated. Each treatment was applied to six plants of cacao. Incidence of monilia infection and fruit weight were monitored every 15 days (in total four periods) after the application of the treatment by spray. Significant differences (P<0.05) were found among treatments for incidence. It was observed that spraying entire cacao trees after two times (approximately 30 days) showed a reduction of monilia infection. After all periods, T1, T2, and T3 showed an incidence of monilia infection by 20.5, 17.7, and 14.9% respectively, compared to cultural control of 41.1%. This reduction of moniliasis infection translates into an increase in fruit weight average for T3 (8.4 kg), T2 (7.3 kg), and T1 (6.9 kg). In contrast, in the control (T3), the fruit weight average decreased by 5.3 kg. Biological control showed efficient management of pathogens as M. roreri. It is recommended to use such antifungal (Aloe vera) spray over at least 120 days which would decrease infection incidence even more.

scholarly journals EKSPLORASI SPATIAL DAN IDENTIFIKASI CENDAWAN ENDOFIT PADA TANAMAN KAKAO (Theobroma cacao L.) DI BALI

2018 ◽  
pp. 1
Author(s):  
Lilis Riana Tambunan ◽  
Meitini Proborini ◽  
Putu Adriani Astiti
Keyword(s):  
Endophytic Fungi ◽  
Theobroma Cacao ◽  
Room Temperature ◽  
Trichoderma Viride ◽  
Raw Material ◽  
Rhizopus Stolonifer ◽  
Pharmaceutical Industries ◽  
Soil Rhizosphere ◽  
The Village ◽  
Theobroma Cacao L

Cocoa plant (Theobroma cacao L.) can grow well in the highlands and lowlands, a plantation and industrial plantwhich is known as one of the export commodities, as raw material for the food and pharmaceutical industries which cancontribute to an increase in foreign exchange endophytic fungus is a fungus living on a live network of plants such asseeds, leaves, flowers, twigs, stems and roots. Truffles are generally mutualistic endophyte and is known to stimulate thegrowth of plants, capable of producing antibiotics and plant growth regulators (hormones). This study aims to determinethe diversity and identify endophytic fungi on Kakao.yang found in two different locations in Bali, in the village of KajaBuahan District of Payangan, Gianyar and the Village of Tua, Marga, Tabanan regency subdistricts. Research carried outin the field with the exploration methods, isolation, identification and diversity analysis conducted at the Laboratory ofPlant Taxonomy (Mycology), Department of Biology, Faculty of Mathematics, University of Udayana. Laboratoryresearch conducted by isolating the roots, leaves, plant stems and content of the soil (rhizosphere) cocoa. Once isolated,grown on PDA medium subsequently incubated at room temperature 250C. Isolates that grow back isolated and purifiedon a PDA medium for further identified to the genera or species level. A total of six isolates of endophytic fungi identifiedin the rhizosphere and cocoa plant that is where four of these isolates were identified to species, two isolates were notidentified until genus. Fungi were identified namely Aspergillus flavus, Rhizopus stolonifer, Cladosporiumsphaeropermum, Trichoderma viride, isolates 1 and 2 isolates.Keywords: cocoa, endophytic fungi, highlands and lowlands

scholarly journals Extractos por destilación de Origanum vulgare, Tradescantia spathacea y Zingiber officinale para el manejo de Moniliophthora roreri de Theobroma cacao

2017 ◽  
Vol 7 (5) ◽  
pp. 1065
Author(s):  
Luz Elena Tamayo España ◽  
Sandra Isabel Ramírez González ◽  
Orlando López Báez ◽  
Ricardo René Quiroga Madrigal ◽  
Saúl Espinosa Zaragoza
Keyword(s):  
Theobroma Cacao ◽  
Zingiber Officinale ◽  
Origanum Vulgare ◽  
Moniliophthora Roreri

El cacao es un cultivo de importancia económica, social y ambiental para el sureste mexicano y de gran renombre mundial dada la calidad de materia prima para la elaboración de chocolates. Sin embargo, existen varios factores que afectan la calidad de los granos de cacao, siendo las enfermedades la principal limitante. La moniliasis (Moniliophthora roreri) es la más peligrosa y destructiva del cultivo, y existen pocas alternativas para el manejo orgánico de esta enfermedad. Por ello, en el presente trabajo se evaluó el efecto inhibitorio de los extractos obtenidos por destilación de Origanum vulgare, Tradescantia spathacea y Zingiber officinale sobre M. roreri. Para lo cual se aisló el hongo en laboratorio; se elaboraron los extractos en planta fresca (300 g L-1 y 600 g L-1) y seca (45 g L-1 y 90 g L-1), cada uno obtenido en dos relaciones agua-alcohol (10:0 y 10:1). Se determinó la concentración mínima inhibitoria (CMI) evaluando crecimiento micelial, conidias totales y germinadas. Se realizó análisis de varianza y comparaciones de medias Tukey (p≤ 0.05%). Los resultados muestran que las tres plantas poseen metabolitos capaces de inhibir el crecimiento y formación de conidias a concentraciones de 50 y 40% tanto en planta fresca como seca, utilizando para la mejor extracción de sus metabolitos alcohol, y su CMI fue de 40%. Para la inhibición total del crecimiento y desarrollo de M. roreri para las tres plantas fueron más eficaces los extractos obtenidos a partir de 300 g de material fresco L-1 y como solvente agua-alcohol relación 10:1.

scholarly journals Extraction and Physicochemical Characterization of Dried Powder Mucilage from Opuntia ficus-indica Cladodes and Aloe Vera Leaves: A Comparative Study

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1689
Author(s):  
María Carolina Otálora ◽  
Andrea Wilches-Torres ◽  
Jovanny A. Gómez Castaño
Keyword(s):  
Ab Initio ◽  
Dietary Fiber ◽  
Aloe Vera ◽  
Three Dimensional ◽  
Physicochemical Characterization ◽  
Fiber Content ◽  
Intramolecular Interactions ◽  
Raw Material ◽  
Opuntia Ficus Indica ◽  
Pharmaceutical Industries

Cactaceae and Asphodelaceae are native desert plants known for their high mucilage content, which is a polysaccharide of growing interest in the food, cosmetic, and pharmaceutical industries. In this study, powdered mucilage was obtained from cladodes of Opuntia ficus-indica (OFI) and aloe vera (AV) leaves, and their molecular, morphological, and thermal properties were investigated and compared. Additionally, their dietary fiber content was determined. Three-dimensional molecular models were calculated for both mucilages using ab initio methods. Vibrational spectra (FTIR and Raman) revealed intramolecular interactions and functional groups that were specified with the help of theoretical ab initio and semi-empirical calculations. SEM micrographs measured at magnifications of 500× and 2000× demonstrated significantly different superficial and internal morphologies between these two mucilages. Thermal analysis using DSC/TGA demonstrated superior thermal stability for the OFI mucilage. The dietary fiber content in OFI mucilage was more than double that of AV mucilage. Our results show that both dehydrated mucilages present adequate thermal and nutritional properties to be used as functional ingredients in industrial formulations; however, OFI mucilage exhibited better physicochemical and functional characteristics than AV mucilage as a raw material.

Processed Gingers: Current and Prospective Use in Food, Cosmetic, and Pharmaceutical Industry

2019 ◽  
Vol 10 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Jae-Suk Choi
Keyword(s):  
Pharmaceutical Industry ◽  
Adverse Effects ◽  
Shelf Life ◽  
Traditional Medicine ◽  
Functional Foods ◽  
Zingiber Officinale ◽  
Raw Material

Ginger (Zingiber officinale) has been widely used in traditional medicine, and as raw material for cosmetics, beverages, and health functional foods. However, some adverse effects of ginger have been reported. In addition, the hot and pungent taste of ginger leads to reduced consumption. To overcome these problems, methods for the production of fermented, steamed (or steamed-dried), aged, roasted and beopje ginger have been developed. This review provides an overview and summary of new ginger processing procedures, including methods to reduce the pungent flavor of ginger, extend shelf-life, increase the health-functional compound content, and improve safety. These attempts might improve the consumption of ginger as well as prospective applications of processed ginger for food, cosmetics, and the pharmaceutical industry, also as discussed in various patents.

scholarly journals Utilization of Cumbeba (Tacinga inamoena) Residue: Drying Kinetics and Effect of Process Conditions on Antioxidant Bioactive Compounds

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 788
Author(s):  
João Paulo de Lima Ferreira ◽  
Alexandre José de Melo Queiroz ◽  
Rossana Maria Feitosa de Figueirêdo ◽  
Wilton Pereira da Silva ◽  
Josivanda Palmeira Gomes ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Fixed Bed ◽  
Drying Kinetics ◽  
Raw Material ◽  
Process Conditions ◽  
Temperature Range ◽  
Water Diffusivity ◽  
Pharmaceutical Industries ◽  
Effective Water

The residue generated from the processing of Tacinga inamoena (cumbeba) fruit pulp represents a large amount of material that is discarded without proper application. Despite that, it is a raw material that is source of ascorbic acid, carotenoids and phenolic compounds, which are valued in nutraceutical diets for allegedly combating free radicals generated in metabolism. This research paper presents a study focused on the mathematical modeling of drying kinetics and the effect of the process on the level of bioactive of cumbeba residue. The experiments of cumbeba residue drying (untreated or whole residue (WR), crushed residue (CR) and residue in the form of foam (FR)) were carried out in a fixed-bed dryer at four air temperatures (50, 60, 70 and 80 °C). Effective water diffusivity (Deff) was determined by the inverse method and its dependence on temperature was described by an Arrhenius-type equation. It was observed that, regardless of the type of pretreatment, the increase in air temperature resulted in higher rate of water removal. The Midilli model showed better simulation of cumbeba residue drying kinetics than the other models tested within the experimental temperature range studied. Effective water diffusivity (Deff) ranged from 6.4890 to 11.1900 × 10−6 m2/s, 2.9285 to 12.754 × 10−9 m2/s and 1.5393 × 10−8 to 12.4270 × 10−6 m2/s with activation energy of 22.3078, 46.7115 and 58.0736 kJ/mol within the temperature range of 50–80 °C obtained for the whole cumbeba, crushed cumbeba and cumbeba residue in the form of foam, respectively. In relation to bioactive compounds, it was observed that for a fixed temperature the whole residue had higher retention of bioactive compounds, especially phenolic compounds, whereas the crushed residue and the residue in the form of foam had intermediate and lower levels, respectively. This study provides evidence that cumbeba residue in its whole form can be used for the recovery of natural antioxidant bioactive compounds, mainly phenolic compounds, with the possibility of application in the food and pharmaceutical industries.

scholarly journals Utilization of Mango, Apple and Banana Fruit Peels as Prebiotics and Functional Ingredients

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 584
Author(s):  
Hafza Fasiha Zahid ◽  
Chaminda Senaka Ranadheera ◽  
Zhongxiang Fang ◽  
Said Ajlouni
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Fruit Weight ◽  
Banana Fruit ◽  
Fruit Peel ◽  
Tropical Fruit ◽  
Functional Ingredients ◽  
Potential Applications ◽  
Mango Peel ◽  
Pharmaceutical Industries ◽  
Physical And Chemical

Among the waste by-products generated by the fruit industry (peels, seeds, and skins), fruit peel constitutes the major component. It is estimated that fruit peel accounts for at least 20% of the fresh fruit weight. Fruit peels are considered as major sources of dietary fiber and anticipated to be successfully utilized as prebiotics. This study examined the chemical composition, functional properties and the prebiotic effects of three major tropical fruit peels (apple, banana and mango). The prebiotic effect was tested using three commercial probiotic strains (Lactobacillus rhamnosus, L. casei and Bifidobacterium lactis) individually and in combination. Each probiotic culture was fortified with different concentration (0%, 2% and 4%) of selected fruit peel powder (FPP). Results revealed that all tested FPP significantly (p < 0.05) enhanced the probiotics viable counts, which reached >10 logs after 24 h of incubation. However, the concentration of 2% and 4% FPP showed no significant differences (p > 0.05) on the probiotic viable counts. Additionally, the prebiotic effects of FPP were the same when applied to individual and mixed cultures. This investigation demonstrated that small amount (2%) of apple, banana and mango peel powder could be successfully utilized as prebiotics to enhance the growth of lactic acid bacteria (LAB). Additionally, the studied physical and chemical characteristics of FPP demonstrated their potential applications in the food and pharmaceutical industries as functional ingredients.

scholarly journals Heterocyclic Aromatic Amines in Meat: Formation, Isolation, Risk Assessment and Inhibitory Effect of Plant Extracts

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1466
Author(s):  
Hafiz Rehan Nadeem ◽  
Saeed Akhtar ◽  
Tariq Ismail ◽  
Piero Sestili ◽  
Jose Manuel Lorenzo ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Plant Extracts ◽  
Aromatic Amines ◽  
Extraction Procedure ◽  
Inhibitory Effect ◽  
Heating Time ◽  
Raw Material ◽  
Heterocyclic Aromatic Amines ◽  
Isolation And Identification ◽  
Cooked Meats

Heterocyclic aromatic amines (HAAs) are potent carcinogenic compounds induced by the Maillard reaction in well-done cooked meats. Free amino acids, protein, creatinine, reducing sugars and nucleosides are major precursors involved in the production of polar and non-polar HAAs. The variety and yield of HAAs are linked with various factors such as meat type, heating time and temperature, cooking method and equipment, fresh meat storage time, raw material and additives, precursor’s presence, water activity, and pH level. For the isolation and identification of HAAs, advanced chromatography and spectroscopy techniques have been employed. These potent mutagens are the etiology of several types of human cancers at the ng/g level and are 100- to 2000-fold stronger than that of aflatoxins and benzopyrene, respectively. This review summarizes previous studies on the formation and types of potent mutagenic and/or carcinogenic HAAs in cooked meats. Furthermore, occurrence, risk assessment, and factors affecting HAA formation are discussed in detail. Additionally, sample extraction procedure and quantification techniques to determine these compounds are analyzed and described. Finally, an overview is presented on the promising strategy to mitigate the risk of HAAs by natural compounds and the effect of plant extracts containing antioxidants to reduce or inhibit the formation of these carcinogenic substances in cooked meats.

scholarly journals Fungicidal effect of silver nanoparticles on toxigenic fungi in cocoa

2016 ◽  
Vol 51 (12) ◽  
pp. 1929-1936 ◽  
Author(s):  
Raquel Villamizar-Gallardo ◽  
Johann Faccelo Osma Cruz ◽  
Oscar Orlando Ortíz-Rodriguez
Keyword(s):  
Silver Nanoparticles ◽  
Fusarium Solani ◽  
Theobroma Cacao ◽  
Mycelial Growth ◽  
Culture Media ◽  
Fungal Growth ◽  
Inhibitory Effect ◽  
Plant Tissues ◽  
Inhibition Effect ◽  
Toxigenic Fungi

Abstract: The objective of this work was to evaluate the microbicidal effect of silver nanoparticles (AgNPs) on potentially toxigenic fungi affecting cocoa (Theobroma cacao) crops. These fungi, isolated from diseased cocoa pods, were characterized phenotypically and genotypically. The microbicidal effect was assessed by measuring radial mycelial growth, in synthetic culture media, and at different AgNP concentrations in plant tissues. The inhibition effect was monitored in Petri dishes, and changes in fungal structures were observed through scanning electron microscopy. Two potentially toxigenic fungi were highly prevalent: Aspergillus flavus and Fusarium solani. The inhibition assays, performed in liquid and solid synthetic culture media, showed that AgNPs did not significantly affect the growth of these fungi, even at the highest concentration (100 ppm). By contrast, they showed a positive inhibitory effect in plant tissues, especially in the cortex, when infected with A. flavus, in which an 80 ppm dose completely inhibited fungal growth. However, once fungi have managed to penetrate inside the pods, their growth is unavoidable, and AgNP effect is reduced. On F. solani, the studied nanomaterial only induced some texture and pigmentation changes. The microbicidal effect of chemically synthesized silver nanoparticles is greater in plant tissues than in culture media.

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