scholarly journals Histology, serum biochemistry and haematological profiles of Clarias gariepinus fed diets containing Luffa cylindrica seedmeal

2020 ◽  
Vol 12 (2) ◽  
pp. 130-139
Author(s):  
W.A. Jimoh ◽  
M.O. Shittu ◽  
A.A. Ayeloja ◽  
S.A. Abdulsalami
Keyword(s):  
Processing Time ◽  
Clarias Gariepinus ◽  
Serum Biochemistry ◽  
Luffa Cylindrica ◽  
Formulated Diets ◽  
Standard Procedures ◽  
Excretory Organ ◽  
Liver And Kidney ◽  
Inclusion Level ◽  
Dietary Treatments

Abstract. A 56-day feeding trial was conducted to evaluate the effect of processing time, inclusion level and/or their interactions on serum biochemistry, haematology and histology of the liver and kidney of Clarias gariepinus fed diets containing Luffa cylindrica seedmeal. The five formulated diets were designated as control (CTR) and tested, replaced at 15 and 30% by 5- and 10-min toasted Luffa cylindrica seedmeal (D515T, D530T, D1015T and D1030T). The experimental design followed a 2×2 factorial experiment in a completely randomised design; the processing time of 5- and 10-min toasting and inclusion level of 15 and 30% serves as factors. Triplicate groups of each treatment were made. Blood sampling, harvesting of organs, serum biochemistry, histology and haematological studies followed standard procedures. The results of the study showed that the effect of processing time, inclusion level and their interactions had significant impact (p<0.05) on Clarias gariepinus. A decrease in RBC, PCV and Hb was observed when compared with control for inclusion level, processing time and/or their interaction. Total protein, albumin and globulin of the blood of fish fed diet CTR was not significantly different (p>0.05) from that of the blood of the fish fed D1015T. Mild to moderate vacuolation of the hepatocytes were recorded among the livers of fish fed control and test dietary treatments except those fed D1030T that recorded very severe vacuolation of the hepatocytes. The kidney, being the excretory organ, was also affected. However, normal cell architecture was recorded in fish fed CTR, D530T and D1030T.

scholarly journals Histopathological changes in liver and kidney of sharptooth catfish fed on cooked Jatropha curcas seedmeal based diets

2020 ◽  
pp. 52-59
Keyword(s):  
Jatropha Curcas ◽  
Standard Procedure ◽  
Control Diet ◽  
Clarias Gariepinus ◽  
Average Weight ◽  
Test Diet ◽  
Histopathological Changes ◽  
Feeding Trial ◽  
Liver And Kidney ◽  
Dietary Treatments

Introduction: Histology of the organs of fish species is important in the understanding of the pathological changes related to nutritional sources. This study evaluates the histopathological alteration in the liver and kidney of sharptooth catfish fed diets containing Jatropha curcas seedmeal in a 56-day feeding trial. Methods: Clarias gariepinus fingerlings of average weight 2.61±0.02g were acclimatized for a week, and allotted into five dietary treatments; containing 0, 25, 50, 75 and 100% Jatropha curcas seedmeal replacement levels for soybean meal respectively. Each treatment was replicated three times with fifteen fish per replicate. Fish were fed 5% body weight on two equal proportions per day. Histological assessment was conducted using standard procedure. Results: There were no lesions on the photomicrographs of the kidney of fish exposed to all the dietary treatments while moderate vacuolation of the hepatocytes was observed in the liver of fish fed diet D1-control diet. There were very prominent melanomicrophage centres in the liver of Clarias gariepinus fed test diet D3 with some hepatocytes which appeared vacuolated. The trend of vacuolation of hepatocytes of the liver among some treatments were not dietary related as no visible lesions were seen in the liver of fish fed test diets D2 ; D4 and D5. Significance: This study established that Jatropha curcas based diets exert hepatoprotective effect on the liver of fish fed with the diets.

scholarly journals Nutrient retention, haematology and serum biochemistry of cockerels fed graded levels of cassava (Manihot esculenta) grit supplemented with moringa (Moringa oleifera) leaf meal

2021 ◽  
Vol 44 (3) ◽  
pp. 314-324
Author(s):  
S. E. Okosun ◽  
S. A. Eguaoje
Keyword(s):  
Control Diet ◽  
Nutrient Retention ◽  
Nutrient Utilization ◽  
Serum Biochemistry ◽  
Nutrient Digestibility ◽  
Crude Fibre ◽  
Mean Values ◽  
Leaf Meal ◽  
Inclusion Level ◽  
Dietary Treatments

A sixteen week trial was conducted to assess the effect of replacing cassava grit supplemented with Moringa leaf meal (MLM) for maize on the nutrient retention, haematology and serum biochemistry of 120 “day old” Harco cockerel chickens. Four experimental cockerel starter and finisher diets were formulated with diet 1 formulated to contain 0% cassava grit while diet 2, 3 and 4 were formulated to contain cassava grit at 33.3, 66.6 and 100% replacement for maize with 5% of moringa leaf meal inclusion in diets 2, 3 and 4 respectively. Chicks were randomly assigned to the four treatment diets in a completely randomized designed (CRD). Result on nutrient digestibility show that crude fibre was significantly (P<0.05) affected by the treatment diets with highest values of 68.21 recorded in birds fed diet 4. Ash and NFE were significantly (P<0.05) influenced by the treatment diets with highest mean values (50.81 and 55.15) observed in birds placed on 100% CGM. Haematological indices revealed that Packed Cell Volume was significantly (P<0.05) highest 32.17% among birds fed the control diet. However, neutrophil was significantly (P<0.05) affected by the treatment diets with highest value 17.67% recorded in birds fed the control diet. Serological study revealed that Albumin value was significantly (P<0.05) influenced by the dietary treatments with highest value (1.60mg/dl) recorded among birds fed 33.3% CGM based diet. Therefore, Cassava grit can replace maize up to 66.6% inclusion level with 5% appropriate supplementation with moringa leaf meal in cockerel diet for optimum nutrient utilization and blood quality.

Intestinal digestibility of selected minerals, growth performance and meat quality in turkeys fed diets supplemented with different sources and levels of zinc

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kamil Otowski ◽  
Aleksandra Drażbo ◽  
Katarzyna Ognik ◽  
Krzysztof Kozłowski
Keyword(s):  
Growth Performance ◽  
Meat Quality ◽  
Redox Status ◽  
Bursa Of Fabricius ◽  
Breast Meat ◽  
Zn Addition ◽  
Inclusion Level ◽  
Dietary Treatments ◽  
Different Sources ◽  
Intestinal Digestibility

AbstractThe aim of this study was to determine whether dietary supplementation with zinc oxide nanoparticales (NP-ZnO) as a substitute for the conventional ZnO affects the intestinal digestibility of selected minerals, growth performance and meat quality in turkeys. The replacement of ZnO with NP-ZnO had no effect on the intestinal digestibility of Zn, Cu, Fe and Ca, whereas the lowest dose of supplemental Zn reduced Zn digestibility. The applied inclusion levels and sources of Zn had no effect on the growth performance (except the feed intake) of turkeys, including liveability. No differences in the relative weights of the heart, spleen and bursa of Fabricius (except the liver), or the weights of the femur and tibia were found between the dietary treatments. Neither the dose nor the source of supplemental Zn influenced carcass dressing percentage or the share of breast, thigh and drumstick muscles in the carcass. In comparison with the highest and moderate doses of Zn, the lowest inclusion level of Zn contributed to increased yellowness of breast meat (P=0.005). The analyzed doses and sources of supplemental Zn exerted varied effects on the redox status of fresh and frozen breast meat. In conclusion, the growth performance of turkeys, carcass yield and composition as well as the redox status of fresh and frozen breast meat were generally similar, regardless of the dietary source and level of Zn. The beneficial effect of Zn addition at 100 mg/kg was improved Zn and Ca digestibility, and increased redness of breast meat.

Haematological and serum biochemical indices of growing rabbits fed graded levels of Yam peel meal as replacement for maize

2020 ◽  
Vol 47 (4) ◽  
pp. 167-175
Author(s):  
I. B. Amaza ◽  
A. Maidala ◽  
C. E. Isidahomen
Keyword(s):  
Biochemical Analysis ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Feeding Trial ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Biochemical Indices ◽  
Dietary Treatments

Haematological and serum biochemical parameters are indicators of physiological, nutritional and pathological status of animals. Investigation of these parameters provide information for diagnostic purposes. The study was conducted to determine the influence of feeding graded levels of yam peel meal (YPM) as a replacement for maize on haematology; blood chemistry was evaluated in rabbits. Thirty- six (New Zealand white X Chinchilla) were randomly assigned to four dietary treatments in a completely randomized design with nine rabbits per treatment. The rabbits were fed diets containing 0, 12.5, 25 and 37.5% of Yam peel meal (YPM) as a replacement for maize, designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were randomly selected for hematological and serum biochemical analysis. All the haematological and serum biochemical indices except AST were affected (P<0.05) by dietary inclusion of YPM. Although most of the parameters investigated were affected by rabbits fed test ingredient, the values fell within the normal literature reference values for rabbits. It can therefore be concluded that up to 37.5% inclusion level of YPM can be fed to growing rabbits without deleterious effects on health status.

Blood Profile and Selected Organ Histopathology of Balami Sheep Fed Shea Cake (Vitellaria paradoxa) Meal

2021 ◽  
pp. 46-56
Author(s):  
D. O. Okunlola ◽  
M. D. Shittu ◽  
O. O. Olatunji ◽  
A. J. Amuda ◽  
B. O. Olateju ◽  
...  
Keyword(s):  
Blood Cell ◽  
Control Diet ◽  
Average Weight ◽  
Blood Profile ◽  
Significant Difference ◽  
Randomized Experimental Design ◽  
Inclusion Level ◽  
Males And Females ◽  
Dietary Treatments ◽  
Representative Samples

The blood profile and histology of selected tissues (kidney, lungs, intestine, and epididymis) of Balami Sheep fed Shea Cake meal supplement was investigated. A total of thirty-two (32) sheep of the average weight range of 11.75-14.75kg comprising an equal number (16) of males and females were randomly allotted to four dietary treatments in a completely randomized experimental design with eight sheep per treatment for twelve weeks duration. Treatment 1 (T1) was a control diet and had zero inclusion level of Shea cake meal (SCM), while treatments 2,3, and 4 had 10%,15%, and 20%  inclusion of SCM, respectively. At the twelfth week of the experiment, two blood samples of 5ml each were taken from each animal from the jugular vein. The first set was preserved in Ethylene Diamine Tetra Acetic Acid (EDTA) bottle for haematological parameters, while the second samples were preserved without EDTA for serum examination. Representative samples of tissues were randomly selected and slaughtered for gross microscopic examination. There was a significant difference (P<0.05) at various levels of inclusion for parameters investigated. The white blood cell (WBC), Red blood cell (RBC), and Packed cell volume (PCV) recorded the highest values at T3 (15% inclusion level) (13.42 x109/L), 2.92x1012/L, 3.75%, respectively). The serum parameters were significantly(p <0.05)different among the treatments. Total protein ranges from 54.78(mg/dl) (T1) - 60.51(mg/dl) (T2), Cholesterol70.15(mg/dl) (T1) - 78.00(mg/dl) (T4). Iron ranges from 58.15(ug/dl) (T1) - 62.88(ug/dl) (T3).The histopathology of tissues (kidney, lungs, intestine, and epididymis) showed no alteration across the treatments (P= .05) in the gross microscopic examinations. No lesion was observed in the lungs, kidney, epididymis, and intestine. The results showed that shea cake meal posed no health risk to Balami sheep and as such recommended as a good supplement in the diets of the sheep.

scholarly journals Antioxidant Properties and Effect of Abrus precatorius Leaves Extract on Haematological and Biochemical Parameters in Rats

2019 ◽  
Vol 13 (2) ◽  
pp. 13-18
Author(s):  
Fatima Mohammad Madaki ◽  
◽  
Adamu Yusuf Kabiru ◽  
Olofu Ogbadoyi Emmanuel ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Herbal Remedy ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Phytochemical Content ◽  
Abrus Precatorius ◽  
Standard Procedures ◽  
Liver And Kidney ◽  
Dpph Scavenging ◽  
Ld50 Value

Background: Abrus precatorius (A. precatorius) is a herbal remedy commonly used in traditional medicine. We investigated the phytochemical, antioxidant, toxic properties, and safety of A. precatorius leaves in rats. Methods: Phytochemical studies were conducted using standard procedures. The antioxidant properties were studied using the reducing power and 2, 2′- diphenyl-1-picrylhydrazyl (DPPH) scavenging assays. The effect of the extract on biochemical and haematological parameters were evaluated after the oral administration of the extract at daily doses of 200, 400 and 600 mg/kg of the rats’ body weight for 28 days. Results: Saponin was the most abundant (202.25±9.25 mg/100g) while alkaloids (9.69±0.34 mg/100g) were the least phytochemical content of A. precatorius. The extract had an LD50 value of >5000 mg/kg, demonstrating significant reducing power and DPPH scavenging activities (IC50 = 106.22 μg/mL). The extract significantly decreased the serum bilirubin and AST concentrations but increased the total proteins concentration compared with the controls (p<0.05). Urea, creatinine, ALP, ALT, chloride and albumin concentrations in the treated rats were not significantly different from those of the controls (p>0.05). The 600 mg/kg dose significantly increased the Hb, PCV, RBC and MCHC of the treated rats, compared to those of the controls (p<0.05). All doses of the extract tested significantly increased the platelet count but did not alter the counts of MCV, MCH, TWBC, RDW, lymphocyte and neutrophils (p<0.05). Conclusions: This study demonstrated that the A. precatorious leaves possess antioxidant and erythropoietic properties. It was also found to be relatively safe with regards to the liver and kidney integrity at concentrations up to 600 mg/kg body weight of the rats.

Growth performance of weaner rabbits on diets containing varying levels of dried okra leaves (Abelmoschus esculentus)

2020 ◽  
Vol 47 (3) ◽  
pp. 124-131
Author(s):  
A. A. Imade ◽  
C. F. I. Onwuka ◽  
V. A. Jegede ◽  
O. Y. Ayo-Ajasa ◽  
B. B. Sarumi ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Average Weight ◽  
Feeding Trial ◽  
Design Data ◽  
Treatment Groups ◽  
Alternative Feed ◽  
Randomized Design ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Dietary Treatments

The need to seek for alternative feed materials with nutritional and medicinal values to support livestock growth necessitated this study. Forty-five unsexed rabbits crosses (Chinchilla × Newzealand white) 5- 6 weeks old with average weight of 720g were used in a 10- week feeding trial to evaluate the performance of growing rabbits fed on varying levels of dried okra leaves. The rabbits were assigned into five treatment groups containing dried okra leaves (DOL) at 0%, 2.5%, 5%, 7.5% and 10% inclusion levels for dietary treatments (T1, T2, T3, T4 and T5) respectively. Each treatment consisted of 9 rabbits and replicated thrice with each replicate consisting of 3 rabbits in a completely randomized design. Data collected were subjected to one-way analysis of variance (ANOVA). Results indicated that daily feed intake was significantly (P<0.05) largest in rabbits fed 5.00% inclusion level of dried okra leaves (84.57g) and least in rabbits fed 0.00% level of inclusion of dried okra leaves (72.12g). Feed efficiency (FE) was poorest (P<0.05) in rabbits fed 10.00% DOL (0.15%) and best (0.20%) in rabbits fed 2.50% dried okra leaves. It was concluded that okra leaves can be included in diets for growing rabbits and 2.50% level of dried okra leaves inclusion is recommended for adoption by farmers to maximize profit.

scholarly journals Lowering dietary cation-anion difference increases sow blood and milk calcium concentrations

2019 ◽  
Vol 97 (7) ◽  
pp. 2927-2939 ◽  
Author(s):  
Ji Yao Guo ◽  
Tiago Junior Pasquetti ◽  
Sung Woo Kim
Keyword(s):  
Parathyroid Hormone ◽  
Serum Biochemistry ◽  
Milk Composition ◽  
Late Gestation ◽  
Acid Base Balance ◽  
Urine Ph ◽  
Blood Ph ◽  
Base Balance ◽  
Dietary Treatments ◽  
Total Alkaline

AbstractA study was conducted to evaluate the effects of feeding an acidogenic diet with a low dietary cation-anion difference (DCAD) on acid-base balance, blood, milk, and urine Ca concentrations of sows during lactation. A total of 30 multiparous sows (parity: 4.5 ± 2.9, Smithfield Premium Genetic, Rose Hill, NC) were allotted to 1 of 2 dietary treatments: CON (control diets were corn-soybean meal based with a calculated DCAD of 170 and 226 mEq/kg during late gestation and lactation, respectively) or ACI (acidogenic diets had a DCAD 100 mEq/kg lower than the control diets). The lower DCAD was achieved by the addition of an acidogenic mineral. The DCAD was calculated as mEq (Na + K – Cl)/kg diet. Sows had a daily access to 2-kg feed from day 94 of gestation to parturition and ad libitum access to feed during lactation. Blood and urine pH and Ca, serum macrominerals, serum biochemistry, Ca-regulating hormones, and milk composition were measured. Sows in ACI had a lower (P < 0.05) blood pH than sows in CON at day 1 of lactation. Sows in ACI had a lower (P < 0.05) urine pH at day 108 of gestation, days 1, 9, and 18 of lactation compared with sows in CON. Sows in ACI had greater (P < 0.05) concentrations of serum total Ca at days 1 and 18 of lactation than sows in CON. There was a greater (P < 0.05) concentration of colostrum Ca in ACI than in CON. There was no difference in urine Ca concentration between treatments during lactation. Concentrations of parathyroid hormone and 1,25-dihydroxycholecalciferol were not different between treatments at either day 1 or 18 of lactation. Sows in ACI tended to have a smaller (P = 0.086) concentration of total alkaline phosphatase in serum at day 18 of lactation compared with sows in CON. At day 1 of lactation, the concentration of serum Cl in ACI was greater (P < 0.05) than that in CON. Feed intake, BW loss, and litter performance were not different between treatments. Collectively, feeding an acidogenic diet with a low DCAD to sows can induce a mild metabolic acidosis at farrowing, reduce the urine pH consistently, and increase serum total Ca and colostrum Ca concentrations during lactation but without altering the parathyroid hormone and 1,25-dihydroxycholecalciferol levels during lactation.

Influence of soybean bioactive peptides on performance, foot pad lesions and carcass characteristics in broilers

2018 ◽  
Vol 6 ◽  
Author(s):  
M. R. Abdollahi ◽  
F. Zaefarian ◽  
Y. Gu ◽  
W. Xiao ◽  
J. Jia ◽  
...  
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Carcass Characteristics ◽  
Biologically Active ◽  
Feed Conversion ◽  
Functional Protein ◽  
Inclusion Level ◽  
Poultry Diets ◽  
Foot Pad ◽  
Dietary Treatments

SummaryThe influence of different inclusion levels of a biologically active peptide derived from soybeans by enzymatic hydrolysis, on growth performance, foot pad lesions and carcass characteristics in broilers were examined in this study. Starter (1 to 21 d) and finisher (22 to 42 d) diets, based on maize and soybean meal, were subjected to seven inclusion levels of a commercial soybean bioactive peptide (SBP) product (Fortide, Chengdu Mytech Biotech Co. Ltd., Chengdu, Sichuan, China) at 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 g/kg of diet. All diets were equivalent in respect of energy density, digestible amino acids and other nutrients. A total of 840, one-day-old male broilers (Ross 308) were allocated to 42 pens (20 birds/pen), which were randomly assigned to seven dietary treatments. During the starter period, there was no significant effect of SBP on weight gain and feed intake of the birds. However, a significant (P < 0.05) effect of SBP was observed for the feed conversion ratio (FCR), with SBP inclusion at 3.0 g/kg and above showing lower (P < 0.05) FCR values compared to the diet with no SBP. No effect of SBP was observed for weight gain and feed intake over the whole trial period. However, SBP inclusion tended (P = 0.06) to influence the FCR of birds. Increasing SBP inclusion level resulted in gradual decrease in FCR values, with SBP inclusion at 5.0 and 6.0 g/kg showing lower FCR values compared to the diet with no SBP. Overall, the present study suggests that dietary supplementation of SBP in broiler diets has the potential to improve FCR and to be used as a novel functional protein in poultry diets.

scholarly journals Tropical kudzu as a protein source in sheep diets

2016 ◽  
Vol 37 (2) ◽  
pp. 933
Author(s):  
Camila Carvalho da Paz ◽  
Aníbal Coutinho do Rêgo ◽  
Cristian Faturi ◽  
Luiz Fernando De Souza Rodrigues ◽  
José Adérito Rodrigues Filho ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Soybean Meal ◽  
Nitrogen Balance ◽  
Dry Matter ◽  
Animal Feed ◽  
Experimental Period ◽  
Neutral Detergent Fiber ◽  
Protein Source ◽  
Formulated Diets ◽  
Inclusion Level

Protein nutrition plays a key role in ruminant performance and represents the largest proportion of costs in animal feed. The present study evaluated whether the roughage portion of Santa Inês sheep diets could be supplemented with different ratios of tropical kudzu as a protein source and assessed the best inclusion level of tropical kudzu based on digestibility, intake, nitrogen balance and feeding behavior. Twenty rams were evaluated during the 20-day experimental period, and they were fed formulated diets with a roughage portion (60%) consisting of Elephant grass and tropical kudzu, the latter supplemented at different concentrations (0, 25, 50 and 75% on a dry matter basis). Forty percent of the concentrate portion consisted primarily of ground corn and soybean meal adjusted to obtain an isoproteic diet. Chemical and bromatological analyses, as well as, analyses to assess intake, digestibility, nitrogen balance and feeding behavior, were conducted. No statistically significant differences were found (P>0.05) for the intake and digestibility of dry matter (DM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), hemicellulose (HEMI), non-fiber carbohydrates (NFC) and total digestible nutrients (TDN) or for the feeding behavior (feeding, rumination and idle times in h/day) and nitrogen balance between the diets studied. Thus, tropical kudzu can be considered a feed alternative to supplementation with prime protein foods (in this case, soybean meal) in sheep diets.

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