scholarly journals Cytological and bacteriological sampling from filters used for embryo recovery to evaluate the uterine status of donor mares

2018 ◽  
Vol 66 (3) ◽  
pp. 462-473 ◽  
Author(s):  
Carlos Carmelo Pérez-Marín ◽  
Guillermo Vizuete ◽  
Carmen Borge ◽  
Juan Jose Galisteo
Keyword(s):  
Clinical Signs ◽  
Inflammatory Cells ◽  
Embryo Recovery ◽  
Three Samples ◽  
The Status ◽  
Uterine Flushing ◽  
Uterine Environment ◽  
The Moment ◽  
Filter Mesh ◽  
Sterile Cotton Swab

While testing for uterine bacterial infection is usually performed prior to artificial insemination (AI), samples taken during or after embryo flushing are generally not assessed either in subfertile and old mares or in fertile mares, even though knowledge of the status of the uterine environment in which the embryo is to develop would help to predict the outcome of embryo transfer programmes. The presence of bacteria and inflammatory cells in the liquid retained in the filter after uterine flushing in donors was determined at the moment of embryo recovery. Primarily, a group of mares (n = 8) displaying evident clinical signs of endometritis was selected to evaluate the cytological and bacteriological findings in filters after uterine flushing and in uterine cotton swabs. Two uterine samples (for cytological and bacterial evaluation) were taken with cotton swabs and, subsequently, the uterus was flushed and the efflux was also subjected to bacteriological and cytological analysis. Later, a group of donors (n = 20) was also involved to evaluate the presence of bacteria and polymorphonuclear leukocytes (PMN). After embryo flushing and collection, the efflux retained in the filter was evaluated by cytology and bacteriology. A sterile cotton swab was then scrubbed on the filter mesh, and a bacterial culture was performed. The embryo recovery rate was 30% (n = 6); Escherichia coli was isolated in one efflux sample collected from embryo-productive flushings, while the other five samples were negative by culture. Bacterial growth (not considered as contamination) was observed in a total of three samples, although no inflammatory cells were detected. Bacteria were isolated in endometrial samples collected after embryo flushing in donor mares, although inflammatory cells were never present in the uterus of mares from which embryos were recovered. In the absence of clinical signs, cytological and/or bacteriological samplings are not very useful for estimating the success of embryo recovery in donor mares, but evaluation of the filter and efflux after uterine flushing in donors may provide valuable information regarding uterine status at embryo collection.

Avascular Necrosis of the Femoral Head in Dogs - Retrospective Study

2018 ◽  
Vol 46 (1) ◽  
Author(s):  
Catarina Borges Cardoso ◽  
Sheila Canevese Rahal ◽  
Maria Jaqueline Mamprim ◽  
Hugo Salvador Oliveira ◽  
Alessandra Melchert ◽  
...  
Keyword(s):  
Femoral Head ◽  
Avascular Necrosis ◽  
Body Mass ◽  
Hind Limb ◽  
Clinical Signs ◽  
Radiographic Appearance ◽  
Hind Limbs ◽  
Grade 5 ◽  
Patient Consultation ◽  
The Moment

Background: The avascular necrosis of the femoral head is a development disease caused by ischemic necrosis, which is mainly observed in young dogs. The etiology of the disease remains controversial. The diagnosis requires imaging exams such as MRI and radiographs. Thus, the aim of the current study was to retrospectively assess a population of dogs with avascular necrosis of the femoral head in order to feature the disease, as well as to analyze the radiographic appearance of the lesion at the moment of patient consultation.Materials, Methods & Results: The signalment factors of dogs (breed, gender, age and body mass), the affected hind limb, the radiographic appearance of the lesion, the clinical signs at the moment of patient consultation, the time of occurrence and the type of treatment were evaluated. The disease was radiographically classified according to the previously described items. Forty-three cases of avascular necrosis of the femoral head were identified, 97.67% presented lameness and pain during palpation of the hip joint; and 54.34%, were 1 week to 4 months old. Females represented 58.13% of the sample, and 65.11% of them weighed from 2.6 to 4.9 kg. Based on the radiographic classification, 4.34% were Grade 1; 32.60%, Grade 2; 8.69%, Grade 3; 19.56%, Grade 4; and 34.78%, Grade 5. The femoral head and neck ostectomy was performed in 42 hind limbs (91.30%); 42.85% of the dogs reached total functional recovery and 26.19% required physiotherapy and rehabilitation.Discussion: The present sample was composed of 25 dogs, which were 6-to-11-month old at the moment of patient consultation, but 17 dogs were 12-to-36-month old at this time; only one dog was older than 36 months. It may be associated with the non-recognition of clinical signs by the owners, rather than with the late-onset form of the disease. With respect to the breed, pinscher, Yorkshire, poodle, Lhasa apso, pug were most frequently observed. However, 7 dogs were crossbreed. Such group differed from that of a review involving 188 cases encompassing West Highland white terrier, Cairn terrier and poodle as the most commonly affected breeds. No sex predilection was found in a study comprising 188 cases, but in another study comprising 14 dogs, female predilection was observed. Likewise, the females represented 58.13% of cases in the present study; and 41.86% of the participants were male. The mean body mass of the dogs in the current study was 4.33 kg, which matches the small breed dogs, which are most affected by the disease. Clinical signs of non-weightbearing lameness or an intermittent subtle lameness are common in the avascular necrosis of the femoral head. Lameness of the affected hind limb was observed in 69.76% of the cases in the present study, but 2.32% showed no lameness. Occasionally, the avascular necrosis of the femoral head is bilaterally found in 12% to 16.5% of the cases. Only 3 out of the 43 cases assessed in the present study were bilateral. Since the lesions were more frequent in Grades 2 and 5, there is less chance of success through the conservative treatment. Thus, the femoral head and neck ostectomy was the surgical procedure performed in 42 hind limbs. The procedure is used to provide pain relief and to reduce the signs of lameness. In conclusion, the population assessed in the present study was composed of small size dogs, mean body mass 4.33 kg, no sex predilection, mostly presenting unilateral lesions and higher Grade 2 and Grade 5 radiographic lesion frequency.Keywords: radiographic, joint, canine, hip.

To the Matter of Procedural and Legal Problems of Detaining a Person on Suspicion of Committing a Crime

2021 ◽  
Author(s):  
E.V. Bolshakov ◽  
◽  
I.D. Nazarov ◽  
Keyword(s):  
Russian Federation ◽  
Criminal Procedure ◽  
Criminal Case ◽  
Judicial Practice ◽  
Procedure Code ◽  
Legal Nature ◽  
The Status ◽  
The Subject ◽  
The Russian Federation ◽  
The Moment

The subject of the research within the framework of the article is the criminal procedure institute for the detention of a person on suspicion of committing a crime. The legal nature of this institution is analyzed, and comments are given on the normative legal acts and judicial practice regulating the issues of detention. The theoretical basis of the research is based on the publications of the last two decades on this problem, in particular, reflecting the discussion of the process scientists S. A. Shafer, S. B. Rossinsky and A. A. Tarasov, the subject of which was the issue of the legal nature of a suspect detention in a criminal case. In the paper, the authors ask the following questions: What is the detention of a person on suspicion of committing a crime in accordance with the legislation of the Russian Federation? From what moment does the detained person acquire the status of a suspect? Is it possible to detain a person before initiating a criminal case? The study concludes that a person acquires the actual status of a suspect from the moment of direct detention, that is, before documenting this status and, as a result, before initiating a criminal case. Amendments to the articles of the Criminal Procedure Code of the Russian Federation are proposed, and the authors` versions of the definitions of the concepts «detention of a suspect», «the moment of actual detention» and «pre-trial proceedings» are given.

PROGRESSIVE ATHEROSCLEROTIC CORONARY ARTERIES IN PATIENTS WITH SEVERAL ISCHEMIC EVENTS IN ANAMNESIS

2021 ◽  
pp. 113-119
Author(s):  
Parkhomenko O.M. ◽  
Lozhkina N.G.
Keyword(s):  
Myocardial Infarction ◽  
Coronary Arteries ◽  
Clinical Signs ◽  
Risk Groups ◽  
Adverse Outcomes ◽  
Syntax Score ◽  
One Year ◽  
The Moment ◽  
Microsoft Office ◽  
Ischemic Events

Вackground. Progressive atherosclerosis is accompanied by unfavorable clinical outcomes; study and understanding of this process is necessary to identify the appropriate risk groups. Purpose of the study to study the dynamics of atherosclerotic lesions of the coronary arteries in patients with several ischemic events in history. Patient Characterization and Research Methods. The present subanalysis included 51 patients with recurrent nonfatal myocardial infarction (MI) out of the initially included 100 patients with index MI. All 100 patients had a history of two or more ischemic coronary or cerebral events, which corresponds to the clinical signs of progressive atherosclerosis. The dynamics of the degree of coronary stenosis from the moment of index MI to repeated MI was assessed according to the data of selective coronary angiography. The statistical program Microsoft Office Excel 2019 was used. Results. All patients with recurrent myocardial infarction (51 people) had signs of progression of coronary artery stenosis: "mild" progression - 82.3%, "moderate" and "severe" - 15.6% and 2.1%, respectively. SYNTAX Score> 22.5 points was a predictor of one-year adverse outcomes: OR 6.349, CI (2.548-15.823). The results obtained make it possible to distinguish a group of patients with accelerated atherosclerosis syndrome in order to stratify the risk and optimally manage this complex category of patients.

scholarly journals Maternity Contract based on the birth for someone else / Materinstvo na osnovu ugovora o rađanju za drugog

2015 ◽  
Vol 5 (5) ◽  
pp. 54
Author(s):  
Илија Бабић
Keyword(s):  
Married Couple ◽  
Civil Code ◽  
Parental Rights ◽  
Surrogate Mother ◽  
Parental Relationship ◽  
Legal Standards ◽  
The Status ◽  
The Moment ◽  
Preliminary Draft

The Draft of the Serbian Civil Code provides for a new contract for the birth for another person, on the basis of which the parental relationship is established. This contract obligates the surrogate mother to carry and give birth to a child and deliver it to the married couple or companions (the intended parents), after impregnation by seeding cells of one or both of the intended parents. The intended parents are required to take the child and establish the parental relationship with the child.The contract can be signed by a woman who lives with a surrogate mother (particularly justified by the reasons and determined on by the court in a contentious procedure - Article 63 of the preliminary draft), when it is necessary to use the seeding cells of the intended mother.The contract on the birth for another person is not in the interest of the child. In the countries where it has been adopted, it represents a means of exploitation of the poorest women and it is unnatural. In the Draft, the contract is regulated mainly according to the general legal standards, whereas the autonomy of the parties involved regulates the rights and obligations (such as the waiver of surrogate mother to the status of mother, the moment of acquisition of parental rights of the intended parents, the handover of the child, reimbursement of reasonable costs, etc).

scholarly journals Nationwide survey reveals high diversity of Fusarium species and related mycotoxins in Brazilian rice: 2014 and 2015 harvests

2019 ◽  
Author(s):  
Gláucia Mara Moreira ◽  
Camila Primieri Nicolli ◽  
Larissa Bitencourt Gomes ◽  
Claudigo Ogoshi ◽  
Klaus K. Scheuermann ◽  
...  
Keyword(s):  
Fusarium Species ◽  
Nationwide Survey ◽  
Morphological Identification ◽  
Mycotoxin Contamination ◽  
Country Level ◽  
Species Complexes ◽  
Three Samples ◽  
The Status ◽  
Concentration Levels ◽  
High Diversity

Ninety three samples of rice were obtained from research and commercial plots at eight rice-producing regions of Brazil and analyzed for the presence of Fusarium species and 14 mycotoxins. A total of 352 isolates belonging to Fusarium genus were obtained from 85 % of the samples. These were assigned to four species complexes (SC) based on morphological identification. The most frequent SC detected was F. incarnatum-equiseti (FIESC, 32.4 %) followed by F. fujikuroi (FFSC, 26.1 %), F. graminearum (FGSC, 24.7 %) and F. chlamydosporum (FCSC, 16.8 %). FGSC was limited geographically and dominant in the southern subtropical production regions while the others occurred in all regions, particularly FIESC, the most widespread among them. The samples were individually contaminated with three to eight mycotoxins. The most common mycotoxins detected were zearalenone (ZEA), beauvericin, and acetylated forms of deoxynivalenol (AcDON). Other toxins included enniatins, T-2, HT-2, DON, neosolaniol and moniliformin. The concentration levels were all below the Brazilian promulgated limits established only for DON (< 750 ppb), and ZEA (< 100 ppb) with one exception for the latter. Most toxins were found in both the husk and flour fractions, but AcDON tended to concentrate more in the husk. Our survey extends considerable our knowledge of the Fusarium complexes infecting rice and provides an update on the status of rice mycotoxin contamination at the country level, which can be considered generally safe. However, attention should be paid to the widespread contamination of beauvericin.

scholarly journals 171IMPROVEMENT IN EMBRYO RECOVERY USING UTERINE DOUBLE FLUSHING

2004 ◽  
Vol 16 (2) ◽  
pp. 207 ◽  
Author(s):  
A. Castro Neto ◽  
B.V. Sanches ◽  
S.H.V. Perri ◽  
M.M. Seneda ◽  
J.F. Garcia
Keyword(s):  
Recovery Rate ◽  
Foley Catheter ◽  
Test Group ◽  
Control Group ◽  
Recovery Rates ◽  
Mean Values ◽  
Embryo Recovery ◽  
Group A ◽  
Uterine Flushing ◽  
The Mean

The aim of the present study was to evaluate the effects of uterine double flushing on embryo recovery rates (total structures and viable embryos), after a resting period with the uterus filled with PBS. There were 210 embryo recovery procedures conducted using the uterine double flushing method, and the results were compaired with 432 conventional single flushing procedures. All procedures were conducted with Limousin (n=403) and Guzerá (n=239) cows, following the same superovulation protocol. Cyclic donors received a progestagen implant (CIDR) and 1mL of oestradiol benzoate (Estrogin®) at Day 0. Between Day 5 and Day 9 animals received doses ranging from 200 to 300UI (zebu cows) and 300 to 500UI (taurine cows) of FSH (Pluset® – Serono) in decreasing doses. Between Day 6 and Day 8 PGF2a (Ciosin®) was administered, followed by withdrawal of the progestagen after 24h. Artificial insemination was performed between 14 and 26h after the beginning of treatment. For the double flushing procedure, after the first uterine flushing of both horns with 1L DPBS (Nutricell), a Foley catheter was positioned in the uterine body in order to fill the uterus with the same solution (80 to 150mL). After this procedure the catheter was closed with a disposable 5-mL syringe gasket, and the animals were allowed to rest in the surroundings of the work place for 30min. After this period, a second flush was performed in order to recover the remaning liquid used during manipulation of the uterus. Animals from the control group (group A) were subjected to a single uterine flushing procedure. From 210 double flushing procedures (group B – test), 1409 viable embryos were produced, classified as grades I, II, III and IV (IETS), (average of 6.7 embryos per procedure), whereas, in the 432 single flushing procedure (group A – control), 1993 embryos were produced (average of 4.6). Statistical analysis showed the increase of viable embryo recovery rate. When consecutive double flushing was performed, the average of recovered embryos increased from 8.3 to 12.7 (P<0.05) in Limousin cows, and from 7.9 to 11.5 (P<0.05) in Guzerá cows. Comparing recovery after single flushing with that after double flushing, the mean number of viable embryos increased from 4.7 to 6.9 (P<0.05) in Limousin cows and from 4.5 to 6.4 (P<0.05) in Guzerá cows. In order to assure the nonexistence of negligence effects or operator influence on results, the mean values of total embryo recovery rate after single flushing (control group) was compared to the mean values of the same rate after the first uterine flushing on test group. Results indicated no difference between recovery rates. The present work showed the viability of using the uterine double flushing procedure for improvement of embryo recovery rates in cattle.

The World Has Been Naked

2021 ◽  
pp. 306-319
Author(s):  
Ismail Noori Msser
Keyword(s):  
Decision Maker ◽  
Human History ◽  
World Today ◽  
The World ◽  
The Status ◽  
Historical Times ◽  
The Moment ◽  
Web Platform ◽  
The Web

After the WikiLeaks incident, the world today is under the shock of the document. Since the moment of the invention of writing and the entry of man in historical times, the document has remained in the context of the closeness of holiness. Yes, human history is witnessing the leaking of more documents of the utmost importance, but the disclosure of this speed and free and direct and defamation only besieged the documents and stamps them with the symbolism missing, after the overthrow of the status of dear and beloved, and disperses prestige on the web platform. It is a departure from the meaning for which the document was historically designed. What was private became commonplace in a confusing way, and it was a secret exposed, and what was stored for 30 years or more, according to the value of the document and its effects, was public before reaching the decision-maker.

Honni soit qui mal y pense

2020 ◽  
pp. 163-216
Author(s):  
Gerd-Rainer Horn
Keyword(s):  
Mass Media ◽  
Status Quo ◽  
Daily Newspapers ◽  
Nazi Occupation ◽  
The Status ◽  
The Moment ◽  
Considerable Detail ◽  
Production Facilities

The challenges to traditional ante-bellum or ante-Mussolini ways of ruling and running societies were perhaps most visible in the area of fundamental changes affecting the most popular mass media at that time: newspapers. Virtually all across Europe, the vast majority of hitherto operating daily newspapers were shut down at the moment of liberation, and a new antifascist press often took over production facilities vacated by their compromised former owners. After some cursory glances at the politics of the post-liberation press in Germany and Italy, I then go into considerable detail in the case of France. For it was in France where the challenges to published opinion in the wake of Nazi occupation went further and deeper than anywhere else. In France, however, too, within very few years the power of money regained the upper hand, turning back the clock to the status quo ante bellum.

Lens and Anterior Uvea

2022 ◽  
pp. 359-392
Keyword(s):  
Macular Edema ◽  
Anterior Uveitis ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Clinical Findings ◽  
Eye Drops ◽  
Systemic Diseases ◽  
Intravitreal Triamcinolone ◽  
Keratic Precipitates ◽  
Intravitreal Triamcinolone Injection

This chapter illustrates photos of clinical signs seen in uveitis and interesting cases of lens pathologies. Anterior uveitis is the inflammation of the iris and the ciliary body. Anterior uveitis can be idiopathic, isolated, or associated with systemic diseases. The clinical findings observed in anterior uveitis include keratic precipitates, inflammatory cells and flare in anterior chamber, hypopyon, rarely hyphema, miosis, iris nodules and atrophy, synechiae, and band keratopathy in chronic cases (shown in corneal degenerations chapter). The inflammation in anterior uveitis is almost always immune. Treatment includes steroid eye drops, cycloplegic drops, sub-Tenon steroid injections when cystoid macular edema is present. Chronic macular edema can be treated with intravitreal Triamcinolone injection and Dexamethasone implants. In cases of refractory anterior uveitis or associated immune systemic diseases, immunomodulatory treatment or biologic agents are prescribed.

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