Thin-layer chromatographic quantification oftrans-resveratrol in cosmetic raw materials of botanic origin

2015 ◽  
Vol 28 (2) ◽  
pp. 167-172
Author(s):  
Marta Skorek ◽  
Violetta Kozik ◽  
Teresa Kowalska ◽  
Mieczysław Sajewicz
Keyword(s):  
Thin Layer ◽  
Raw Materials ◽  
Botanic Origin

Analysis of morphological features and preliminary assessment of arbutin content in food varieties of pears

2021 ◽  
pp. 64-71
Author(s):  
Nadezhda V. Nesterova ◽  
Albina Dosaeva
Keyword(s):  
Thin Layer ◽  
Raw Materials ◽  
Raw Material ◽  
Diagnostic Study ◽  
Preliminary Assessment ◽  
Promising Alternative ◽  
Modern Methods ◽  
The Russian Federation ◽  
Color Reactions ◽  
Layer Chromatography

In this article, the authors conducted a macro-diagnostic study of pear fruits of three varieties. A qualitative analysis of raw materials was carried out using color reactions, thin-layer chromatography in pear fruits most widely cultivated in the territory of the Russian Federation. Arbutin was identified in the raw material, which allows us to consider this type of raw material as a promising alternative to lingonberry and bearberry leaves used in official medicine and actualizes further research aimed at developing modern methods of standardization with subsequent inclusion in the developed regulatory documentation.

scholarly journals Simple, At-Site Detection of Diethylene Glycol/Ethylene Glycol Contamination of Glycerin and Glycerin-Based Raw Materials by Thin-Layer Chromatography

1998 ◽  
Vol 81 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Allen S Kenyon ◽  
Shi Xiaoye ◽  
Wang Yan ◽  
Ng Wai Har ◽  
Robert Prestridge ◽  
...  
Keyword(s):  
Thin Layer ◽  
Diethylene Glycol ◽  
Visual Inspection ◽  
Raw Materials ◽  
World Health ◽  
Visual Method ◽  
Laboratory Facilities ◽  
Training Samples ◽  
Health Organization ◽  
Layer Chromatography

Abstract This paper describes a rapid, inexpensive thin-layer chromatographic (TLC) method that separates diethylene glycol (DEG) from glycerin and other glycols. Studies with collaborating laboratories of the World Health Organization have shown that about 6% DEG in glycerin and about 2% DEG in acetaminophen (paracetamol) elixirs may be detected by direct visual inspection of the developed TLC sheets. Staining the sheet permits detection of DEG at less than 0.1%. The method costs less than $1.00 per test and takes 20 min by visual inspection, longer when staining is required. The visual method can be performed without laboratory facilities by personnel having little previous training. Samples testing positive by the visual method can be submitted to a laboratory for confirmation and quantitation of DEG.

Microwave Synthesis and Properties of Thin Layer Pt Modified TiO2 Nanofibers Coating

2016 ◽  
Vol 721 ◽  
pp. 383-388
Author(s):  
Reinis Drunka ◽  
Jānis Grabis ◽  
Dzidra Jankovica ◽  
Aija Krūmiņa
Keyword(s):  
Thin Layer ◽  
Surface Area ◽  
Visible Light Irradiation ◽  
Microwave Synthesis ◽  
Raw Materials ◽  
Synthesis Method ◽  
Total Surface Area ◽  
Microwave Assisted Synthesis ◽  
Fiber Layer ◽  
Microwave Assisted

In the present work formation of macro-size TiO2 nanofiber layers in microwave synthesis were studied. Anatase nanopowder and 10M KOH solution were used as raw materials. Microwave assisted synthesis method permitted to obtain TiO2 nanofibres containing individual nanowires with a diameter of 5nm and a specific surface area up to 129m2/g. Macro size layer of TiO2 nanofibers was obtained on the surface of microwave vessel. Total surface area covered with TiO2 was about 390cm2. Modification with platinum allows to increase photocatalytical activity of TiO2 macro size fiber layer up to 94.4% in UV irradiation and up to 11.6% under visible light irradiation.

scholarly journals Determination of deoxynivalenol in feed, feed raw materials and food

2020 ◽  
Vol 64 (12) ◽  
pp. 28-33
Author(s):  
Nailya N. Mishina ◽  
◽  
Ivan N. Shtyrov ◽  
Zukhra H. Sagdeeva ◽  
Ramziya M. Potekhina ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Russian Federation ◽  
Raw Materials ◽  
Food Products ◽  
Technical Documentation ◽  
Veterinary Services ◽  
The Russian Federation ◽  
Layer Chromatography

The article presents the survey data of 1830 average samples of feed, feed raw materials and food products received through the Test Center of the FSBSI «FCTRBS-RRVI»", provided by livestock and feed enterprises, agricultural companies, food manufacturers and 152 samples of feed provided by specialists of veterinary services , livestock enterprises, owners of peasant farms to determine the death of animals and birds, for the content of mycotoxin deoxynivalenol (DON) in the period from 2018 to 2020. Determination of the DON content in the samples was carried out according to a certified procedure based on the method of thin layer chromatography. In the course of summarizing the results, it was found that samples of feed, feed raw materials and food products received through the Test Center of the of the FSBSI «FCTRBS-RRVI» from different regions of the Russian Federation in terms of DON content corresponded to the normative indicators specified in the current regulatory and technical documentation. When determining the cause of death of animals and birds, 152 samples were examined, received from farms of different regions of the Republics of Tatarstan, Bashkortostan, Mordovia, Kostroma and Ryazan regions. It was found that 1.97% of samples were contaminated with DON at concentrations from 0.2 to 0.5 mg/kg of feed, in the rest, the content of mycotoxin was below the sensitivity of the method (< 0.2 mg/kg), which does not exceed the limit permissible concentration. It was recommended to exclude feed contaminated with mycotoxin from the diet of animals, since at the established concentrations, DON is not the main etiological factor in the death of animals, but due to the presence of carcinogenic, mutagenic, teratogenic, embryotoxic and immunosuppressive properties, together with other factors, it is contributing.

scholarly journals Scientific-methodological approach for standardization of herbal raw materials and herbal preparations using high-performance thin-layer chromatography procedure

2021 ◽  
pp. 76-89
Author(s):  
К. О. Хохлова
Keyword(s):  
Quality Control ◽  
Thin Layer ◽  
Related Species ◽  
High Performance ◽  
Raw Materials ◽  
Methodological Approach ◽  
Herbal Drugs ◽  
Closely Related Species ◽  
Layer Chromatography

The high-performance thin-layer chromatography (HPTLC) is widely used for quality control of herbal raw materials (HRM). The purpose of this work were to define the problematic issues of quality control of herbal drugs in Ukraine and to develop a systematic scientific and methodological approach for the standardization of HRM and herbal drugs using the modern analytical technique of HPTLC: to optimize the existing and develop new methods of identification and determination of adulterations, and to develop alternative methods of quantitative determination, as well as studying of the stability. As a result of a critical evaluation of existing approaches for the standardization of HRM and herbal drugs of Ukraine’s flora, numerous problematic issues were identified, including the existence of a limited number of national monographs / national parts of monographs on the HRM of the Ukraine’s flora, which were not previously standardized by other pharmacopeias; non-consideration of Ukraine’s flora species (non-pharmacopoeial) in monographs on closely related species that introduced in the State Pharmacopoeia of Ukraine; lack of specificity of combinations of physical and chemical methods used for quality control; lack of specificity and reproducibility, the labor intensity of methods; necessity of usage of toxic solvents and precursors; use of different groups of markers and methods for the HRM and its finished product or HRM and its closely related species, etc. The proposed systematic scientific and methodological approach includes: a preliminary collection of information about the object of study, markers/groups of bioactive substances, approaches to its standardization; theoretical evaluation of existing techniques of quality control in monographs on HRM and experimental approbation using samples of domestic plants, determination of the need for optimization of existed methods by the parallel development of specific, reproducible and documented methods for identification, adulterations’ detection, quantification, and bio-detection (if necessary) by HPTLC method. The approach is illustrated with examples. The proposed approach can be used for comprehensive HPTLC analysis of domestic HRM and herbal drugs for research, prevention of adulteration, standardization of initial HRM and its finished product, pharmaceutical development, and stability studies.

Determination of Geographical Origin of Spices. I. Cassia and Cinnamon by Thin Layer Chromatography

1967 ◽  
Vol 50 (4) ◽  
pp. 852-856
Author(s):  
William A Voelker ◽  
Jean N Skarzynski ◽  
William H Stahl
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Geographical Origin ◽  
Raw Materials ◽  
Screening Method ◽  
Solvent System ◽  
Dimensional System ◽  
Quality Control Tool ◽  
Ceylon Cinnamon ◽  
The One

Abstract A thin layer chromatographic method has been devised to differentiate the geographical origin of cinnamons (cassias). For the one-dimensional screening method, benzene-ethyl acetate-glacial acetic acid (90: 10:1, v/v/v), was used as the solvent system. The two-dimensional system first used hexane-acetone (85:15) and then benzene- ethyl acetate (85:15). The following products were differentiated on the basis of fluorescing spots, some spots common to all and some spots unique: Saigon cassia, Batavia cassia, Korintji cassia, Ceylon cinnamon, and Seychelles cinnamon. The technique is primarily designed as a quality control tool to evaluate incoming shipments of raw materials. However, it can he used to determine composition of unknown mixtures of the ground cinnamons. The technique permits judgment within ± 2 0 % of one type in a mixture

scholarly journals ÓLEOS ESSENCIAIS E VEGETAIS: MATÉRIAS-PRIMAS PARA FABRICAÇÃO DE BIOPRODUTOS NAS AULAS DE QUÍMICA ORGÂNICA EXPERIMENTAL

Química Nova ◽  
2021 ◽  
Author(s):  
Catarina Bruno ◽  
Márcia Almeida
Keyword(s):  
Organic Chemistry ◽  
Green Chemistry ◽  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Vegetable Oils ◽  
Raw Materials ◽  
Natural Dye ◽  
Class 1 ◽  
Layer Chromatography

ESSENTIAL AND VEGETABLE OILS: RAW MATERIALS FOR THE MANUFACTURE OF BIOPRODUCTS IN CLASSES IN EXPERIMENTAL ORGANIC CHEMISTRY. A schedule was prepared for three undergraduate classes for the discipline of experimental organic chemistry for obtaining bioproducts. As natural raw materials, lavender flowers and cinnamon peels were used to obtain hydrolates and essential oils (Class 1), coconut and avocado pulp to obtain vegetable oils and annatto seeds to obtain a natural dye (Class 2 ). Cosmetic bioproducts (Class 3), body emulsions similar to those found on the market, were obtained from the mixture of essential and vegetable oils and the addition of natural dye. The raw materials obtained can also be used in other classes of the discipline, complying with the principles of Green Chemistry. Essential oils, for example, can be used as samples for Thin Layer Chromatography (TLC) classes and vegetable oils for the production of biodiesel.

Thin-layer chromatographic identification of flavonoids and phenolic acids contained in cosmetic raw materials

2016 ◽  
Vol 39 (5-6) ◽  
pp. 286-291 ◽  
Author(s):  
Marta Skorek ◽  
Karolina Jurczyk ◽  
Mieczysław Sajewicz ◽  
Teresa Kowalska
Keyword(s):  
Thin Layer ◽  
Phenolic Acids ◽  
Raw Materials

scholarly journals Validation of High-Performance Thin-Layer Chromatographic Methods for the Identification of Botanicals in a cGMP Environment

2008 ◽  
Vol 91 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Eike Reich ◽  
Anne Schibli ◽  
Alison DeBatt
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Raw Materials ◽  
Black Cohosh ◽  
Licorice Root ◽  
Ginseng Root ◽  
Validation Process ◽  
Aerial Parts ◽  
Good Manufacturing Practices ◽  
Tea Leaf

Abstract Current Good Manufacturing Practices (cGMP) for botanicals stipulates the use of appropriate methods for identification of raw materials. Due to natural variability, chemical analysis of plant material is a great challenge and requires special approaches. This paper presents a comprehensive proposal to the process of validating qualitative high-performance thin-layer chromatographic (HPTLC) methods, proving that such methods are suitable for the purpose. The steps of the validation process are discussed and illustrated with examples taken from a project aiming at validation of methods for identification of green tea leaf, ginseng root, eleuthero root, echinacea root, black cohosh rhizome, licorice root, kava root, milk thistle aerial parts, feverfew aerial parts, and ginger root. The appendix of the paper, which includes complete documentation and method write-up for those plants, is available on the J. AOAC Int. Website (&lt;ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://www.atypon-link.com/AOAC/loi/jaoi"&gt;http://www.atypon-link.com/AOAC/loi/jaoi&lt;/ext-link&gt;).

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