Simultaneous recovery of pectin and colorants from solid agro-wastes formed in processing of colorful berries

2011 ◽  
Vol 7 (1) ◽  
pp. 65-80
Author(s):  
Petra Cserjési ◽  
Katalin Bélafi-Bakó ◽  
Zsófia Csanádi ◽  
Sándor Beszédes ◽  
Cecilia Hodúr
Keyword(s):  
Antioxidant Activity ◽  
Human Health ◽  
Hot Water ◽  
Total Phenol ◽  
Anthocyanin Content ◽  
Black Currant ◽  
Color Coordinates ◽  
Pectic Substances ◽  
Different Color ◽  
Environmentally Sound

Extraction of pectic substances from solid agro-wastes and the application of recovered pectin in the food, pharmaceutical and cosmetic industry can significantly contribute to a more economic and environmentally sound agro-industrial production. Thus investigation of the physicochemical properties of extracted pectic substances seems important not only from human health preservation considerations, but their advantageous properties can be confirmed for the possible manufacturers, potential processing, as well. Therefore, in this work pectic substances were extracted from red currant, black currant, raspberry, blackberry and elderberry press residues by hot water and the composition, antioxidant activity, total phenol, anthocyanin content and the color coordinates of pectins were determined. The results show that the pectin colors fall in the range between reddish purple (black currant and elderberry) and yellow (citrus and apple). Moreover some of the pectins in a powder form have different color coordinates than in the form of aqueous solutions or gels. This might be very important when the pectins are selected for different production processes. The anthocyanin content of pectin preparations is lower than the values of fruit juices. It can be concluded that the colorants found in pectin preparations belong to the group of phenolics and have adequate antioxidant capacity, which is extremely beneficial for human health. As a summary it was concluded that the investigated pectins can be easily extracted and successfully used as natural colorants or antioxidants since they have adequate antioxidant activity, total phenol and anthocyanin content and suitable color coordinates.

scholarly journals Elaboration of novel extraction procedure to reveal bioactive component profile of anthocyanin-rich plants 

2014 ◽  
Vol 32 (No. 4) ◽  
pp. 384-390
Author(s):  
A. Kiss ◽  
S. Rapi ◽  
M. Korozs ◽  
P. Forgo
Keyword(s):  
Antioxidant Activity ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Phenolic Content ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Hot Water ◽  
Anthocyanin Content ◽  
Black Currant ◽  
Dpph Method

The content of anthocyanin derivatives, antioxidant activity, and phenolic content were determined in black elderberry (Sambucus nigra L.), sweet cherry (Prunus avium L.), blackberry (Rubus fruticosus L.), black currant (Ribes nigrum L.), and blackthorn (Prunus Spinosa L.). The extraction efficiency was examined of several solvents including hot water, 2% phosphoric acid, ethanol and acetone. A new sequential (cascade) extraction procedure was developed in order to improve the efficiency of the conventional methods. This novel extraction protocol consists of 3 different steps with the prevalence of low pH extraction conditions. When comparing the effectiveness of the conventional and presently improved procedures, it was stated that significantly increased anthocyanin yields had been observed. The highest anthocyanin content, determined with HPLC method, was found in the case of sweet cherry (222.7 mg/kg) on using the three step extraction procedure. The highest antioxidant activity determined with DPPH method was also assigned to the sweet cherry sample (5272 mg/kg). The highest phenolic content was found in blackberry (434 mg/kg).

scholarly journals Extraction, Evaluation, and Antioxidant Activity of Total Phenol from Callus of Abutilon indicum (L.) Sweet

2021 ◽  
Vol 11 (3) ◽  
pp. 3652-3660
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Water Extract ◽  
Hot Water ◽  
Total Phenol ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Layer Chromatography ◽  
Abutilon Indicum

In this present study, callus induction from the leaves of Abutilon indicum was performed and evaluated antioxidant potential after TLC, PTLC. For induction of callus, Murashige, and Skoog (MS) medium with 2.5 mg/L concentration of 2, 4 Dichloro phenoxy acetic acid (2,4-D) and for assay, Folin Ciocalteau method practiced for evaluation of total phenol, modified spectrophotometer method used for DPPH assay. Based on total phenolic content, total antioxidant capacity, DPPH, assay, and reducing power assay performed after separation and partial purification of total phenols by Thin-layer chromatography (TLC) and Preparative thin-layer chromatography (PTLC), respectively. For total phenol estimation, hot water extract shows the highest total phenol content (10.56 μg/ml) by comparing another extract; similarly, PTLC fraction of callus extract from Abutilon indicum shows 61% of inhibition in DPPH at 10 μg/ml. From this study, compared to leaf, callus shows a higher concentration of total phenol and shows promising antioxidant activity. So, it gives scope for further exploration and purification and application of total phenol from callus of Abutilon indicum.

scholarly journals Karakteristik Fisikokimia Tepung Ubi Banggai dan Aplikasinya Dalam Beras Analog

2021 ◽  
Vol 8 (1) ◽  
pp. 43-52
Author(s):  
Aini Auliana Amar ◽  
Feri Kusnandar ◽  
Slamet Budijanto
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Amylose Content ◽  
Total Phenol ◽  
Yam Flour ◽  
Cooling Phase ◽  
Different Color ◽  
Extrusion Technology ◽  
Physical And Chemical ◽  
Central Sulawesi

Banggai yam (Dioscorea sp) has many varieties, which is locally cultivated and consumed by people in Banggai, Central Sulawesi. Banggai yam can be processed into flour and applied as an ingre-dient in analogue rice. This study aimed to evaluate the physical and chemical composition, total phenol and antioxidant activity of banggai yam flours, and the physical properties, total phenol and antioxidant activity of their analogue rice. Five varieties of banggai yams (Harum, Banggai, Pasus, Doso, and Penus) were compared. Banggai yam flours were composed of moisture (12.21-12.74%wb), ash (2.18-2.75%db), protein (10.41-11.68%db), fat (1.05-3.38%db), and carbohydrate (83.22-85.77%db). All banggai flours had different color (L, a, and b values) intensities, and contained starch (65.38-68.40% db) with amylose content of 38.96-45.09% (db). The total phenol ranged from 30.48-116.90 mg GAE/ 100 g, and showed antioxidant activity (58.71-89.50% inhibition). The pasting profile varied among five yam flours. The initial pasting temperatures of banggai yam flour were relatively high (82.5-83.7°C). All banggai yam flour increased in viscosity up to a peak and experienced slight viscosity breakdown during the heating phase, as well as showed a setback viscosity during cooling phase. Rice analogue of yam flours made by extrusion technology had a relatively low bulk density (0.47-0.56 g/mL), and required a long cooking time (7-9 minutes). The rice analog had different levels of total phenol and antioxidant activities. The level of total phenol and antioxidant activity of analogue rice from Harum yam showed the highest compared to those other analogue rice.

scholarly journals Total phenol, flavonoid, and anthocyanin content and antioxidant activity of Etlingera elatior extract and nanoparticle

Pharmaciana ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 145
Author(s):  
Tresna Lestari ◽  
Tita Nofianti ◽  
Lilis Tuslinah ◽  
Ruswanto Ruswanto
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Anthocyanin Content ◽  
Etlingera Elatior

CARBOHYDRATE PRODUCT FOR THERAPEUTIC AND PROPHYLACTIC PURPOSES

2020 ◽  
pp. 89-94
Author(s):  
Khvorova L.S. ◽  
Byzov V.A.
Keyword(s):  
Antioxidant Activity ◽  
Uric Acid ◽  
Phenolic Compounds ◽  
Mental Stress ◽  
The Body ◽  
Hot Water ◽  
Preventive Effect ◽  
Activity Inhibition ◽  
Medicinal Properties ◽  
Simple Carbohydrates

The article is devoted to the creation of a carbohydrate product with rosehip extract in the form of sweets (glucose Fudge) with a therapeutic and preventive effect aimed at restoring the body of people exposed to intense physical and mental stress and the effects of damaging environmental factors. As carbohydrates in the recipe, simple carbohydrates (glucose) and complex carbohydrates in the form of starch molasses containing (maltose, tri - Tetra - sugars, dextrins) are used. Glucose in the formulation is the main energy ingredient, sweetener and structure-forming component that gives the product a solid consistency due to crystallization. The medicinal effect of the product is provided by rosehip extract. Numerous published studies have established its vitamin, immunostimulating and antioxidant activity, inhibition of uric acid formation and obesity, which are associated with flavonoids, other phenolic compounds, and vitamins. The list of medicinal properties of rosehip extract shows the feasibility of using it in additional nutrition of people in order to prevent diseases. For the formulation of our sweets, the extraction of crushed rosehip fruits was carried out three times with hot water at a temperature of 65-700C, followed by concentration of the extract under vacuum to 25-30% SV. The resulting extract-concentrate was introduced into the formulation in a dosage of 5.5% SV per 100 g of SV glucose Fudge, which provided the necessary dosage for the product when used from 3 (for children) to 10 sweets a day (for adults) for the prevention of colds.

Variation of Phytochemicals Content in Pulp and Skin of Seven Romanian Apple Cultivars

2017 ◽  
Vol 68 (3) ◽  
pp. 474-477
Author(s):  
Lacramioara Oprica ◽  
Doina Atofanei ◽  
Vladimir Poroch
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anthocyanin Content ◽  
Colour Intensity ◽  
Total Polyphenol ◽  
Valuable Source ◽  
Golden Delicious ◽  
Apple Cultivars ◽  
Apple Fruits

The amounts of anthocyanins, flavonoids, total polyphenol and ascorbic acid in seven apple cultivars from Romania were investigated. The amounts of polyphenol and flavonoids in pulp and skin of apple cultivars ranged between 17.18�7.52 mg GAE/g DW and 20.10�11.06 mg CE/g DW as well as 3.64�1.18 mg GAE/g DW and 10.31�5.57 mg CE/g DW, respectively. The highest and smallest values of ascorbic acid both in the skin and the pulp were observed in the cultivars Mutsu and Starkrimson. The anthocyanin content is positively correlated with the colour intensity of the apple epicarp, being about three-fold higher in Prima than Golden Delicious cultivar. The better antioxidant activity provided by the content of polyphenol, ascorbic acid and flavonoids was in apple fruits of Mutsu cultivar and for this reason it should be regarded as a valuable source of antioxidants.

Screening of Extremophile Microalgae Isolated from High Background Radiation Areas as Source of Bioactive Materials

2020 ◽  
Vol 16 (4) ◽  
pp. 407-414
Author(s):  
Fatemeh Heidari ◽  
Zeinab Shariatmadari ◽  
Hossein Riahi
Keyword(s):  
Antioxidant Activity ◽  
Hot Springs ◽  
Background Radiation ◽  
Cadmium Stress ◽  
Total Phenol ◽  
Total Phenolic ◽  
Extreme Conditions ◽  
Unicellular Cyanobacterium ◽  
High Background ◽  
Cadmium Adsorption

Background: Microalgae are the source of various compounds with high potentials for being used in different industries. The production of such compounds can be raised under extreme conditions. In the present study, four cyanobacteria and one coccoid green alga were examined which were isolated from hot springs in high background radiation areas in Ramsar, a city in the north of Iran. Methods: Cadmium adsorption from aqueous solution, response towards cadmium stress, antioxidant activity, total phenolic compound and drought tolerance were investigated in these microalgae. Results: The results showed that these extremophile microalgae contain valuable biological compounds which can be useful in remediation of heavy metals from contaminated water and soils and pharmaceutical applications. The unicellular cyanobacterium, Chroococidiopsis thermalis IBRC-M50002, was the best strain with the highest biological activity in various testes such as cadmium adsorption (225 mg g-1), cadmium tolerance stress (100 mg ml-1), antioxidant activity (IC50= 18 μg mg-1) and total phenol content (100 μg ml-1). The coccoid green algae Grasiella emersonii IBRC-M50001, also exhibited significant antioxidant activity (IC50=10 μg mg-1) and total phenol compound (116 μg ml-1), but its cadmium adsorption, tolerance at cadmium stress and desiccation were lower than Chroococidiopsis thermalis. Conclusion: HBRAs microalgae, isolated from extreme conditions, are useful microorganisms for the production of bioactive substances and natural antioxidants. In other words, they exhibited high capacity to be used in pharmaceutical, industrial and commercial applications.

scholarly journals IDENTIFIKASI SENYAWA FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KASAR DAUN PEPE

2019 ◽  
Vol 8 (2) ◽  
pp. 111
Author(s):  
Dewa Gede Eka Prayoga ◽  
Komang Ayu Nocianitri ◽  
Ni Nyoman Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Data ◽  
Qualitative Data ◽  
Total Phenol ◽  
Total Flavonoids ◽  
Randomized Design ◽  
Solvent Type ◽  
Ic50 Values ◽  
Phytochemical Compounds ◽  
Completely Randomized Design

This research was conducted to identify the phytochemical compounds and antioxidant activity of pepe leaves (Gymnema reticulatum Br.) crude extract in various solvent types. The experimental design used in this research was a completely randomized design with solvent type treatments consisting of 5 types : aquadest, ethyl acetate 96%, acetone 70%, ethanol 70%, and methanol 95%. The treatment was repeated three times, so that 15 experimental units were obtained. Qualitative data obtained in this research were displayed with tables, while quantitative data were analyzed by variance and if the treatment had a significant effect, then followed by Duncan's test. The results showed that the treatment of solvent types affected phytochemical compounds that was extracted from pepe leaves and had a significant effect (P<0.01) on yield, total phenol, total flavonoids, and antioxidant activity. The best results showed that 70% acetone solvents were able to extract phytochemical compounds from alkaloids, saponins, phenols, flavonoids, and steroids in the form of sterols and had the highest antioxidant activity based on 62.06% free radical inhibition percentage with IC50 values of 200.775 mg/L, yield 32.35%, total phenol 36.14 mgGAE/g extract, and total flavonoids 50.37 mgQE/g extract.

Comparative study of not from concentrate and reconstituted from concentrate of pomegranate juices on nutritional and sensory profile

2021 ◽  
pp. 108201322110037
Author(s):  
G Brunda ◽  
Urs Kavyashree ◽  
Shilpa S Shetty ◽  
Kirti Sharma
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Essential Elements ◽  
Iron Bioavailability ◽  
Sensory Characteristics ◽  
Sensory Profile ◽  
Pomegranate Juice ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Hepg2 Cell Lines

Pomegranate juice is popular due to its unique health benefits, sensory characteristics and also a good source of bioactive compounds. Comparative study on processing effect of Not from Concentrate (NFC) and Reconstituted from concentrate (RFC) pomegranate juice on the nutritional and sensory characteristics of ‘Ganesh’ variant was conducted. Results showed that not much differences observed in parameters like pH, acidity, essential elements, protein, total sugars and polyphenol content between NFC and RFC. As per the study NFC had a better antioxidant activity with intracellular ROS inhibition of 11% higher with significant ( p < 0.05) than RFC in HepG2 cell lines. Total anthocyanin content was significantly different ( p < 0.05) in NFC (428.05 mg/l) compared to 326.74 mg/l in RFC expressed as cyanidin-3-glucoside. Iron uptake was 40 units (µg/mg protein) higher in NFC than RFC ( p < 0.05) in HepG2 cells. Sensory flavor profile showed NFC having significant differences with respect to characteristic pomegranate freshness, fruitiness, sweetness and astringency mouthfeel. RFC had higher sweetish and cooked flavor with additional vegetable like notes of beet and carrot. Based on the data better antioxidant activity, iron bioavailability, anthocyanin content and sensory attributes were captured in pomegranate NFC juices over RFC juices.

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