scholarly journals IMPROVEMENT OF NON SPECIFIC IMMUNE SYSTEM ON COMMON CARP (Cyprinus carpio) AGAINST KOI HERPESVIRUS DISEASE (KHVD) BY ADDITION OF ASCORBIC ACID ON FISH DIET: A FIELD SCALE STUDY

2010 ◽  
Vol 5 (1) ◽  
pp. 45
Author(s):  
Taukhid Taukhid ◽  
Desy Sugiani ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Common Carp ◽  
Stocking Density ◽  
Control Fish ◽  
Control Group ◽  
Fish Diet ◽  
Feeding Frequency ◽  
Koi Herpesvirus ◽  
Daily Application

The research with the aim to know the optimal feeding frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) on the dose of 750 mg/kg feed to control Koi Herpesvirus (KHV) disease infecting common carp has been done in field condition. Fish were reared in floating cages with the size of 3.5 m x 3.5 m x 2.0 m and stocking density of 1,250 fish/cage with the size range of ± 10 g/fish. The treatments applied in the research were: (A) daily application, (B) every 3 days application, and (C) without vitamin C addition as the control. Fish test were challenged to KHV infection on the mid cultivation by cohabitation method in the laboratory scale for 2 weeks. Examination on behavior, clinical sign, and mortality of fish test conducted daily. The results showed that the highest survival rate was found on the application of vitamin C every 3 days (60.16%); and followed by every day (52.00%), and the lowest was found on the control group (47.36%).

Effect of Dietary Ascorbate on the Concentration of Tissue Ascorbic Acid, Dehydroascorbic Acid, Ascorbic Sulfate, and Activity of Ascorbic Sulfate Sulfohydrolase in Rainbow Trout (Oncorhynchus mykiss)

1990 ◽  
Vol 47 (8) ◽  
pp. 1518-1525 ◽  
Author(s):  
Konrad Dabrowski ◽  
Reinhard Lackner ◽  
Cristine Doblander
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Dehydroascorbic Acid ◽  
Control Group ◽  
High Demand ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dietary Treatments ◽  
Kidney Liver

The concentrations of ascorbic acid in several tissues of rainbow trout (Oncorhynchus mykiss) are significantly influenced by various dietary treatments. Ascorbic acid was taken up readily by erythrocytes, kidney, liver, intestine, spleen, and brain in fish fed an ascorbate supplemented diet (AA group), the concentration being from 1.5 to 14.8-fold higher than in fish fed a diet lacking ascorbate (control group). In fish fed a diet supplemented with an equimolar amount of ascorbic acid in the form of ascorbic sulfate (AS group) the ascorbic acid concentrations in kidney, intestine, and erythrocytes were significantly elevated above those of the control group. Ascorbic sulfate was found in kidney, liver, and intestine of the AS group, but not in other groups. In fish fed a diet devoid of vitamin C the ascorbic acid concentrations in kidney, liver, intestine, and spleen were signficantly lower than in fasting fish over the same period of time (28 d), suggesting a high demand for vitamin C in an actively feeding animal. Salmonid fish are therefore probably unable to utilize ascorbic sulfate sufficiently to prevent the appearance of vitamin C deficiency, and thus resemble scurvy-prone mammals in this respect.

Evaluation of the toxic effect of silver nanoparticles and the possible protective effect of ascorbic acid on the parotid glands of albino rats: An in vivo study

2020 ◽  
Vol 36 (6) ◽  
pp. 446-453
Author(s):  
Salma Awad Taghyan ◽  
Hend El Messiry ◽  
Medhat Ahmed El Zainy
Keyword(s):  
Silver Nanoparticles ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Protective Effect ◽  
Toxic Effect ◽  
Toxic Effects ◽  
Control Group ◽  
Albino Rats ◽  
Parotid Glands ◽  
Ductal Cells

This study aimed to evaluate the toxic effect of silver nanoparticles (AgNPs) on the parotid glands (PGs) of albino rats histologically and ultrastructurally and assess the possible protective effect of ascorbic acid as an antioxidant. Thirty male albino rats weighing between 150 mg and 200 mg were divided into three groups: the control group (C1) contained 10 rats that received 2 mg/kg (body weight (bw)) of aqueous nitrate buffer by intraperitoneal (IP) injection daily for 28 days; the AgNPs group contained 10 rats that received 2 mg/kg (bw) IP AgNPs daily for 28 days; and the AgNPs-vitamin C group contained 10 albino rats that received 2 mg/kg (bw) AgNPs IP daily for 28 days with oral administration of 100 mg/kg (bw) vitamin C in drinking water daily for 28 days. The PG acinar and ductal cells of the AgNPs group showed signs of toxicity and degeneration characterized as pleomorphic nuclei, binucleation, cytoplasmic vacuolations, and stagnated secretion in the ductal lumen. In addition to degenerated mitochondria, dilated rough endoplasmic reticulum and lysosomes were filled with AgNPs ( p < 0.001). The AgNPs-vitamin C group showed significantly less degenerative changes histologically and ultrastructurally compared to the AgNPs group ( p = 0.002). AgNPs produced significant toxic effects on the PG of albino rats, presumably through the generation of reactive oxygen species and toxic ion release, and administration of vitamin C was shown effective in decreasing these toxic effects.

scholarly journals The administration of vitamin C in a large institution and its effect on general health and resistance to infection

1942 ◽  
Vol 42 (1) ◽  
pp. 1-19 ◽  
Author(s):  
A. J. Glazebrook ◽  
Scott Thomson
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Common Cold ◽  
Test Group ◽  
Treated Group ◽  
Control Group ◽  
Duration Of Illness ◽  
Large Institution ◽  
Resistance To Infection ◽  
Excretion Level

1. The vitamin C in the dietary of an institution was largely destroyed by the methods of cooking and distribution.2. Some 50 mg. of ascorbic acid per head per day were required to be added to the diet to produce an optimum excretion level.3. Large doses of ascorbic acid were given to a group of adolescents in the institution over a period of several months. A record was kept of the incidences of infectious diseases in this treated group and in the remainder (controls). The following conclusions were reached:(a) The incidences of common cold and tonsillitis were the same in the two groups.(b) The average duration of illness due to the common cold was the same in the two groups.(c) The duration of illness of tonsillitis was longer in the control group than in the test group.(d) Cases of rheumatic fever and pneumonia occurred in the control group but no case of either disease occurred in the test group.

scholarly journals EFEKTIVITAS PENAMBAHAN VITAMIN C (ASCORBIC ACID) PADA PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPESVIRUS (KHV) PADA IKAN MAS, Cyprinus carpio

2016 ◽  
Vol 5 (3) ◽  
pp. 425
Author(s):  
Taukhid Taukhid ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Survival Rate ◽  
Vitamin C ◽  
Clinical Symptoms ◽  
Control Group ◽  
Commercial Diet ◽  
Treatment Groups ◽  
Koi Herpesvirus ◽  
B Vitamin ◽  
Different Doses ◽  
All Treatment

Riset dengan tujuan untuk mengevaluasi teknik pengendalian penyakit KHV pada ikan mas melalui penggunaan imunostimulan yaitu penambahan vitamin C pada pakan komersial dengan dosis yang berbeda telah dilakukan pada skala laboratorium. Perlakuan yang diterapkan adalah penambahan vitamin C jenis CFC-90 (microencapsulated vitamin C) pada pakan komersial dengan berbagai dosis yaitu: (A) tanpa vitamin C, sebagai kontrol, (B) vitamin C sebanyak 250 mg/kg pakan, (C) vitamin C sebanyak 500 mg/kg pakan, (D) vitamin C sebanyak 750 mg/kg pakan, dan (E) vitamin C sebanyak 1.000 mg/kg pakan. Masing-masing perlakuan dilakukan pengulangan sebanyak 3 (tiga) kali. Pada hari ke-15, seluruh kelompok perlakuan diinfeksi KHV secara buatan dengan teknik kohabitasi. Pengamatan dilakukan terhadap tingkah laku, gejala klinis, dan mortalitas ikan uji yang dilakukan setiap hari hingga akhir percobaan. Rataan persen sintasan ikan uji tertinggi pada akhir riset diperoleh pada penambahan vitamin C sebanyak 750 mg/kg pakan yaitu sebesar 82,22%; diikuti dengan penambahan vitamin C 250 mg/kg pakan sebesar 70,00%; vitamin C 1.000 mg/kg pakan sebesar 61,11%; vitamin C 500 mg/kg pakan sebesar 58,89%; sedangkan rataan sintasan terendah diperoleh pada kelompok kontrol yaitu sebesar 27,78%.Research with the aim to evaluate the technique of KHV disease control carp through the use of immunostimulatory namely the addition of vitamin C in commercial diet with different doses have been performed in a laboratorial scale. The treatment applied was the addition of vitamin C of CFC-90 (microencapsulated vitamin C) in the commercial diet with different doses, namely: (A) without vitamin C, as control, (B) Vitamin C 250 mg/kg feed, (C) vitamin C of 500 mg/kg feed, (D) Vitamin C 750 mg/kg feed, and (E) of vitamin C as much as 1,000 mg/kg feed. Each treatment was repeated three times. On day 15th, all treatment groups were artificially infected with KHV using cohabitation techniques. Observations conducted to monitor behavior, clinical symptoms and mortality of fish tests were done every day until the end of the experiment. The highest mean percent of survival rate was recorded in treatment D by adding vitamin C as much as 750 mg/kg of feed that was equal to 82.22%, followed by the addition of vitamin C 250 mg/kg diets at 70.00% (treatment B), Vitamin C 1,000 mg/kg feed amounted to 61.11% (treatment E), vitamin C 500 mg/kg diets at 58.89% (treatment C), while the lowest average of survival rate was obtained in the control group that was equal to 27.78%.

scholarly journals THE EFFECT OF VITAMIN C AND AEROMONAS VACCINE ON THE IMMUNE RESPONSE AND DISEASE RESISTANCE OF GROUPER (Epinephelus fuscoguttatus)

2009 ◽  
Vol 34 (2) ◽  
pp. 81-85
Author(s):  
Ilmiah ◽  
St. Hidayah Triana ◽  
Asmi Citra Malina A.R. Tassakka ◽  
Alex Rantetondok ◽  
Hilal Anshary
Keyword(s):  
Vitamin C ◽  
Immune Responses ◽  
Phagocytic Activity ◽  
Head Kidney ◽  
Treated Group ◽  
Aeromonas Salmonicida ◽  
Control Fish ◽  
Control Group ◽  
Epinephelus Fuscoguttatus ◽  
Titre Antibody

We evaluated the effectiveness of vitamin C and Aeromonas salmonicida vaccine in grouper (Epinephelus fuscoguttatus) for increasing immune responses and protection against A .salmonicida. The vitamin C used was polyethoxylated ascorbic and tocopherol. The vaccine was prepared from formalin-killed cells and concentrated extracellular products of a single isolate A. salmonicida. Bath immersion vitamin C and vaccine trials were conducted for 60 min. Fish used had a mean weight 25 g. Control groupers were injected with tryptic soy broth. The results showed that vitamin C enhanced phagocytic activity in head kidney leucocytes of grouper 7, 14, 28 and 36 days after treatments. A significant different of the antibody titre was found between control fish and the treated fish at 42 days after treatments. In addition, at day 42, Relative Percent Survival (RPS) for control group was 53.3 %, vitamin C-treated group was 80.0 % and vaccinated group was 90.0 %. The results of this study suggest that bath immersion of vitamin C provided an increasing of phagocytic activity (non-specific immune responses), titre antibody (specific immune responses) and protection against A. salmonicida infection in grouper. A. salmonicida vaccine also en-hanced titre antibody and protection against A. salmonicida infection in grouper.

scholarly journals Evaluation of Hatchability from Various Intraspecific Crosses of Carp and Effect of Frequencies of Feeding on Stocking Density of Common Carp Fry

2019 ◽  
Vol 1 ◽  
pp. 88-95
Author(s):  
O O Jacob ◽  
G S Solomon ◽  
P A Annune
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Stocking Density ◽  
Fertilization Rate ◽  
Feeding Frequency ◽  
Male And Female ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Common carp (Cyprinus carpio) germplasm were sourced from Bauchi and Ibadan aimed at studying their hatchability from the various intraspecific crosses and effect of frequencies of feeding on stocking density of Carp fry. Both male and female were injected with Ovaprim at 0.5ml/kg body weight intramuscularly at a single dose. The number of eggs in 1 gram mass was determined, Fertilization rate and percentage of hatchability were calculated. One hundred and eighty (180) fry of Cyprinus carpio were randomly picked and distributed in nine bowls of 50 litres at three stocking densities with replicates for each treatment. The frequencies of feeding were in these order; Ten (10) fry per bowl of 50 litres were fed twice a day, Twenty ( 20) fry per bowl of 50 litres were fed four times a day, Thirty (30) fry per bowl of 50 litres were fed six times a day, respectively for 8 weeks with 30gm of 0.2mm Coppens starter diet. The Ib♀ x Ba♂ cross had the highest hatchability though highest fertilization rate was recorded by Ba♀ X Ba♂ cross. The various crosses had above 50% hatchability, though percentagehatchability differed significantly at (P <0.05). While the growth pattern of the different stocking densities at different feeding frequencies differed significantly (p<0.05). The intra specific crossing of different sexes from the two sources gave a higher yield as can be seen with the IB♀ x BA♂. Whereas carp fry stocked at a lower stocking density with a fewer feeding frequency had best growth.

scholarly journals The Effect of Vitamin C on Lead-induced Plasma BiochemicalAlterations in Fish, Cyprinus carpio

2019 ◽  
Vol 13 (2) ◽  
pp. 25-29
Author(s):  
Kimiya Nourian ◽  
◽  
Hassan Baghshani ◽  
Davar Shahsavani ◽  
◽  
...  
Keyword(s):  
Vitamin C ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Lead Acetate ◽  
Lead Toxicity ◽  
Significant Rise ◽  
Control Group ◽  
Protective Effects ◽  
Plasma Parameters ◽  
Group 2

Background: This study was conducted to investigate whether vitamin C can protect against plasma biochemical changes induced by lead poisoning in Cyprinus carpio. Methods: Three groups of common carp (n=30/group) were used in this study. Group 1 served as control, Group 2 was exposed to lead acetate (5 mg/L) for 15 days, and Group 3 received vitamin C (500 mg/kg) during the same duration of lead exposure. Results: The blood lead concentrations in Groups 2 and 3 showed a significant rise as compared to that in the control group (p<0.05). Also, the plasma malondialdehyde (MDA) concentration increased considerably following in groups exposed to lead acetate, compared to that in controls. Vitamin C supplementation decreased the rise in plasma MDA insignificantly, compared that in Group 2. The plasma values of creatinine and alanine aminotransferase (ALT) increased in Group 2 compared to that in controls. Also, vitamin C treatment significantly decreased plasma ALT and creatinine concentrations, compared to those in Group 2. Values of other plasma parameters including aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, gammaglutamyl transferase, albumin, glucose, total protein, cholesterol, urea, uric acid and triglyceride showed no significant alterations among the treatment groups. Conclusion: The results suggest that vitamin C have some beneficial effects against lead toxicity in common carp. However, elucidation of the precise mechanism of the protective effects of vitamin C against lead toxicity warrants further investigations

scholarly journals Effect of mix probiotic and vitamin C on growth and some biochemical tests in common carp Cyprinus carpio L

2011 ◽  
Vol 35 (1) ◽  
pp. 141-152
Author(s):  
Jamal Khalaf Atiyah AL-Faragi
Keyword(s):  
Growth Rate ◽  
Vitamin C ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Control Group ◽  
Biochemical Tests ◽  
Blood Haemoglobin ◽  
Total Growth ◽  
Group 2 ◽  
Vit C

One hundred and twelve Common carp fish fingerling Cyprinus carpio L.were collected, range between 25-28 gm in weight to study the effect of different concentrations of local probiotic and mixed with Vitamin C in concentration 1g/kg feed on growth performance, blood and biochemical picture. This experiment included 7 groups each group contain 16 fish distributed randomly as follow: Control group (C ) fed diet free vitamin C and probiotic during the period of 60 days and all total protin in feed reach 26% . Group 2(T1) group fed diet contain Vit C (1gm /kg) food. Treated (T2andT3) groups fed diet containing probiotic 4.5&5.5 g/ kg respectively , where as groupsT4,T5 and T6 fed the diets containing Probiotic added at level 4.5,5.5 and 6.5 g/ kg respectively and each mixed with 1 gm\kg Vit C .Results of fish total growth rate were 6.86,9.82,15.00,10.87,17.51,14.60 and 13.51gm, evaluate relative growth rate ,the absolute specific growth rate, food conversion and the protein efficiency ratio . The statistically analysis showed increase significantly(P≤0.05)for T4 in all treatment. While erythrocyte counts was 1.80, 1.88, 1.95, 2.40, 2.00, 2.70, 2.20 and 2.10 ×106 cells\mm3, blood cells counts are 24.40 ,24.60 ,25.80 ,27.00 ,26.00 ,27.96 ,26.60 and 26.20×103cells\mm3, blood haemoglobin was8.40,8.70,9.80, 11.10,10.20,11.90,10.80 and 10.50 gm\100dl, haematocrit value are 27.10 ,27.60 ,28.50 ,29.90 ,29.10 ,30.50 ,29.50 and29.30 % for fish before starting expermint (Z) and C,T1,T2,T3,T4,T5,T6,groups, respectively . Evaluate the enzymatic activity for AST,ALT and ALP in blood serum of fish . The statisticaly analysis showed decrease significantly(P≤0.05) at treatment contain mix probiotic and Vit C but excel T4 inform 70.40,10.40,30.30 I U\ L respectively.Conclusion ,the present studied suggested that the level of probiotic and Vit C (4.5g\kg and Vi C 1gm\kg) used could be improve the performance growth and fish healthy.

scholarly journals Effect of Terbuthylazine-2-hydroxy at Environmental Concentrations on Early Life Stages of Common Carp (Cyprinus carpioL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Josef Velisek ◽  
Alzbeta Stara ◽  
Dalibor Koutnik ◽  
Jana Machova
Keyword(s):  
Common Carp ◽  
Early Life ◽  
Life Stage ◽  
Early Life Stage ◽  
Control Fish ◽  
Control Group ◽  
Embryo Viability ◽  
Sod Activity ◽  
Environmental Concentration ◽  
Effect Concentration

The aim of the study was to investigate effects of the triazine’s herbicide terbuthylazine-2-hydroxy on early life stage of common carp (Cyprinus carpioL.) through antioxidant indices, mortality, growth, development, and histopathology. Based on accumulated mortality in the experimental groups, lethal concentrations of terbuthylazine-2-hydroxy were estimated at 35-day LC50 = 10.9 mg/L terbuthylazine-2-hydroxy. By day 15, fish were exposed to 3.5 mg/L and by day 26, fish were exposed to 0.0029 mg/L; real environmental concentration in Czech rivers, 0.07 mg/L, 1.4 mg/L, and 3.5 mg/L terbuthylazine-2-hydroxy, showed significantly lower mass and total length compared with controls. Based on inhibition of growth in the experimental groups, lowest observed effect concentration (LOEC) = 0.002 mg/L terbuthylazine-2-hydroxy and no observed effect concentration (NOEC) = 0.0001 mg/L terbuthylazine-2-hydroxy. No significant negative effects on hatching or embryo viability were demonstrated at the concentrations tested, but significant differences in early ontogeny among groups were noted. Fish from the two highest tested concentrations showed a dose-related delay in development compared with the controls. Total superoxide dismutase (SOD) activity was significant lower in all groups testedly for terbuthylazine-2-hydroxy compared with the control group. At concentrations of 1.4 and 3.5 mg/L damage to caudal kidney tubules when compared to control fish was found.

SUPPLEMENTAL VALUE OF ASCORBIC ACID DURING LATE GESTATION ON PIGLET SURVIVAL AND EARLY GROWTH

1983 ◽  
Vol 63 (3) ◽  
pp. 683-687 ◽  
Author(s):  
E. R. CHAVEZ
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Late Gestation ◽  
Dietary Vitamin ◽  
Control Group ◽  
Plasma Vitamin ◽  
Significant Difference ◽  
Vitamin C Supplementation ◽  
Dietary Treatments ◽  
Piglet Survival

Twenty-six pregnant sows were included in four different dietary treatments as follows: (1) Control group with no vitamin C supplementation; (2) vitamin-C-supplemented group receiving 1 g/day ascorbic acid provided in a gelatin capsule; or (3) vitamin-C-supplemented group receiving 1 g/day ascorbic acid in a powder form spread on the top of the pelleted meal; (4) as (3) but receiving 10 g/day ascorbic acid. Vitamin-C-supplemented sows in treatments 2 and 4 showed a significant (P < 0.05) increase in blood plasma vitamin C prefarrowing. Pregnant sows receiving 10 g/day vitamin C supplement showed a significantly (P < 0.05) higher concentration of this vitamin in the urine than either control or sows receiving 1 g/day vitamin C supplementation. Sows in treatment 4 showed a significantly higher (P < 0.05) vitamin C content in colostrum than control animals. During lactation, no significant difference in vitamin C content in milk was observed between control and animals receiving 10 g/day vitamin C supplement. The vitamin C content in the plasma of the piglets at birth indicated that supplementing vitamin C to the mothers, significantly (P < 0.05) increased the concentration of this vitamin in the newborn piglet compared to control animals. However, the piglet survival and growth up to weaning at 3 wk of age was not significantly affected by dietary vitamin C supplementation of the mothers' diet. Key words: Ascorbic acid, gestation, piglet survival, Vitamin C

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