Antibodies to Der p 1 and Der p 2 in allergic patients

Introduction and objectives: Atopic individuals are characterized by increased IgE production and Th2 response if exposed to certain antigens. It is known that the mother transfers antimite antibodies to the fetus and newborn, IgG thru the placenta, and IgA thru breastfeeding, but it is not clear whether there is a protective mechanism mediated by them concerning the development of future allergies. This study aimed to compare the levels of IgA, IgG, and IgE antibodies specific to Der p 1 and Der p 2 between atopic and healthy individuals.Methods: Serum samples of 98 patients and 44 healthy controls were subjected to quantification for specific IgE, IgG, and IgA antibodies against Der p 1 and Der p 2 by ImmunoCap® and ELISA, and subjected to statistical analysis as indicated.Results: Atopic patients had higher serum levels of IgE, IgG, and IgA specific to Der p 1 and Der p 2. The correlation was more robust between IgE and IgG antibodies.Conclusions: Allergic patients produce higher levels of antibodies against Der p 1 and Der p 2 compared with healthy individuals. The mechanisms involved still require detailed studies.

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Extracellular Vesicle-Based Detection of Pancreatic Cancer

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.697939 ◽

2021 ◽

Vol 9 ◽

Author(s):

Yesim Verel-Yilmaz ◽

Juan Pablo Fernández ◽

Agnes Schäfer ◽

Sheila Nevermann ◽

Lena Cook ◽

...

Keyword(s):

High Sensitivity ◽

Roc Curves ◽

Serum Levels ◽

Extracellular Vesicle ◽

Ductal Adenocarcinoma ◽

Rank Test ◽

Healthy Controls ◽

Precursor Lesions ◽

Healthy Individuals ◽

Serum Samples

Due to a grim prognosis, there is an urgent need to detect pancreatic ductal adenocarcinoma (PDAC) prior to metastasis. However, reliable diagnostic imaging methods or biomarkers for PDAC or its precursor lesions are still scarce. ADAM8, a metalloprotease-disintegrin, is highly expressed in PDAC tissue and negatively correlates with patient survival. The aim of our study was to determine the ability of ADAM8-positive extracellular vesicles (EVs) and cargo microRNAs (miRNAs) to discriminate precursor lesions or PDAC from healthy controls. In order to investigate enrichment of ADAM8 on EVs, these were isolated from serum of patients with PDAC (n = 52), precursor lesions (n = 7) and healthy individuals (n = 20). Nanoparticle Tracking Analysis and electron microscopy indicated successful preparation of EVs that were analyzed for ADAM8 by FACS. Additionally, EV cargo analyses of miRNAs from the same serum samples revealed the presence of miR-720 and miR-451 by qPCR and was validated in 20 additional PDAC samples. Statistical analyses included Wilcoxon rank test and ROC curves. FACS analysis detected significant enrichment of ADAM8 in EVs from patients with PDAC or precursor lesions compared to healthy individuals (p = 0.0005). ADAM8-dependent co-variates, miR-451 and miR-720 were also diagnostic, as patients with PDAC had significantly higher serum levels of miR-451 and lower serum levels of miR-720 than healthy controls and reached high sensitivity and specificity (AUC = 0.93 and 1.00, respectively) to discriminate PDAC from healthy control. Thus, detection of ADAM8-positive EVs and related cargo miR-720 and miR-451 may constitute a specific biomarker set for screening individuals at risk for PDAC.

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Molecular Allergen Profiling of Dual Mite Sensitization in Severe Allergic Rhinitis

Journal of Investigational Allergology and Clinical Immunology ◽

10.18176/jiaci.0439 ◽

2020 ◽

Vol 30 (6) ◽

pp. 421-429 ◽

Cited By ~ 1

Author(s):

R González-Pérez ◽

F Pineda ◽

P Poza-Guedes ◽

M Castillo ◽

V Matheu ◽

...

Keyword(s):

Allergic Rhinitis ◽

Specific Ige ◽

Indoor Allergens ◽

Serum Samples ◽

Patient Centered ◽

Specific Serum ◽

Mite Allergy ◽

Der P 1 ◽

Skin Prick Tests ◽

Der P 2

Background: Mites are the most prevalent source of indoor allergens. The present study used a component-resolved diagnosis (CRD) approach to investigate the mite-specific IgE sensitization profile for Dermatophagoides pteronyssinus and Blomia tropicalis. We also assessed the performance of a commercially available CRD approach in patients with severe allergic rhinitis. Methods: We selected 63 consecutive patients with dual sensitization to D pteronyssinus and B tropicalis and persistent severe rhinitis according to the ARIA guidelines. We performed skin prick tests with standardized extracts and determined specific serum IgE to both mites, along with serum specific IgE to Der p 1, Der p 2, Der p 23, Der p 10, and Blo t 5. Results: Fifty-eight and 59 patients had positive sIgE to the whole extracts of D pteronyssinus and B tropicalis, respectively. While 91.67% of patients were sensitized to specific IgE to Der p 1, Der p 2, and/or Der p 23, specific IgE to Blo t 5 (≥0.3 ISU-E) was not detected in most of the serum samples (55%). Conclusions: Although the combination panel of the commercially available major allergens Der p 1, Der p 2, and Der p 23 identified more than 90% of the D pteronyssinus–allergic patients, Blo t 5 performed somewhat poorly in those sensitized to B tropicalis. Improvements in CRD and further research concerning the prevalence and clinical relevance of serodominant allergens are needed to achieve a genuine molecular diagnosis, as well as patient-centered mite allergy–specific immunotherapy.

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Value of immune factors for monitoring risk of lung cancer in patients with interstitial lung disease

Journal of International Medical Research ◽

10.1177/0300060519847403 ◽

2019 ◽

Vol 47 (7) ◽

pp. 3344-3353

Author(s):

Ning Li ◽

Haisheng Hu ◽

Ge Wu ◽

Baoqing Sun

Keyword(s):

Lung Cancer ◽

Interstitial Lung Disease ◽

Lung Disease ◽

Serum Levels ◽

Scaling Analysis ◽

Healthy Controls ◽

Healthy Individuals ◽

Reactive Protein ◽

Increased Risk ◽

Immune Factors

Objective Patients with interstitial lung disease (ILD) are at increased risk of developing lung cancer. We aimed to investigate the clinical significance of serum immune factors in this progression. Methods We retrospectively screened a hospital database from January 2012 to December 2016 for patients with lung cancer and ILD. We measured serum levels of C3, C4, IgA, IgG, IgM, C-reactive protein (CRP), ceruloplasmin (CER), and rheumatoid factor in these patients and in healthy controls. Results We analyzed data for 262 patients with lung cancer, 220 with ILD, and 57 healthy controls. CER levels were significantly higher in patients with lung cancer (0.35 ± 0.10 g/L) compared with both ILD patients (0.31 ± 0.25 g/L) and healthy individuals (0.25 ± 0.04 g/L). C3 and C4 levels were both significantly higher in healthy individuals compared with patients with lung cancer (C3: 1.70 ± 0.29 vs 1.04 ± 0.26 g/L, C4: 0.27 ± 0.24 vs 0.24 ± 0.09 g/L) and ILD (C3: 1.70 ± 0.29 vs 0.97 ± 0.25 g/L, C4: 0.27 ± 0.24 vs 0.21 ± 0.09 g/L). Optimal scaling analysis demonstrated that lung cancer was closely associated with CRP, CER, C3, and C4. Conclusions Increased levels of CRP and CER and decreased levels of C3 and C4 may identify patients with ILD at high risk of developing lung cancer.

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A possible relationship between Thromboxane B2 and Leukotriene B4 and the encapsulation of Dirofilaria repens worms in human subcutaneous dirofilariasis

Journal of Helminthology ◽

10.1017/s0022149x19000464 ◽

2019 ◽

Vol 94 ◽

Cited By ~ 1

Author(s):

R. Morchón ◽

E. Carretón ◽

R. García ◽

T. Zueva ◽

V. Kartashev ◽

...

Keyword(s):

Leukotriene B4 ◽

Dirofilaria Repens ◽

Thromboxane B2 ◽

Healthy Controls ◽

Healthy Individuals ◽

Chain Reaction ◽

Serum Samples ◽

Clinical Presentations ◽

Local Inflammatory Reaction ◽

Polymerase Chain

Abstract Human subcutaneous dirofilariosis has several clinical presentations. Many cases present as subcutaneous nodules, as a consequence of a local inflammatory reaction that encapsulates and destroys the worms. In addition, there are cases in which migrating worms located in the ocular area remain unencapsulated. In the present work, the levels of two pro-inflammatory eicosanoids, thromboxane B2 (TxB2) and leukotriene B4 (LTB4) are analysed by commercial Enzime-Linked immunosorbent assay (ELISA) in serum samples from 43 individuals, 28 diagnosed as having subcutaneous dirofilariasis presenting a subcutaneous nodule, five diagnosed as having dirofilariasis, in which the worms remained unencapsulated in the periphery of the eye, and ten healthy individuals living in a non-endemic area, used as controls. The worms were surgically removed, identifying Dirofilaria repens as the causative agent in all cases, by Polymerase Chain Reaction (PCR). Individuals with nodules showed significantly higher levels of TxB2 and LTB4 than healthy controls, whereas significant differences in LTB4 levels were observed between individuals with unencapsulated worms and healthy controls. It is speculated that the absence of LTB4 may contribute to the fact that worms remain unencapsulated as a part of immune evasion mechanisms.

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Reduced serum levels of pro-inflammatory chemokines in Fragile X Syndrome

10.21203/rs.2.13398/v3 ◽

2020 ◽

Author(s):

Anke Van Dijck ◽

Susana Barbosa ◽

Patricia Bermudez-Martin ◽

Olfa Khalfallah ◽

Cyprien Gilet ◽

...

Keyword(s):

Fragile X Syndrome ◽

Robust Regression ◽

Fragile X ◽

Serum Levels ◽

Unsupervised Clustering ◽

Healthy Controls ◽

Healthy Individuals ◽

Clustering Method ◽

Inflammatory Chemokines ◽

Healthy Control

Abstract Background: Fragile X syndrome (FXS) is the most frequent cause of inherited intellectual disability and the most commonly identified monogenic cause of autism. Recent studies have shown that long-term pathological consequences of FXS are not solely confined to the central nervous system (CNS) but rather extend to other physiological dysfunctions in peripheral organs. To gain insights into possible immune dysfunctions in FXS, we profiled a large panel of immune-related biomarkers in the serum of FXS patients and healthy controls. Methods: We have used a sensitive and robust Electro Chemi Luminescence (ECL)-based immunoassay to measure the levels of 52 cytokines in the serum of n=25 FXS patients and n=29 healthy controls. We then used univariate statistics and multivariate analysis, as well as an advanced unsupervised clustering method, to identify combinations of immune-related biomarkers that could discriminate FXS patients from healthy individuals. Results: While the majority of the tested cytokines were present at similar levels in FXS patients and healthy individuals, nine chemokines, CCL2, CCL3, CCL4, CCL11, CCL13, CCL17, CCL22, CCL26 and CXCL10, were present at much lower levels in FXS patients. Using robust regression, we show that six of these biomarkers (CCL2, CCL3, CCL11, CCL22, CCL26 and CXCL10) were negatively associated with FXS diagnosis. Finally, applying the K-sparse unsupervised clustering method to the biomarker dataset allowed for the identification of two subsets of individuals, which essentially matched the FXS and healthy control categories. Conclusions: Our data show that FXS patients exhibit reduced serum levels of several chemokines. This paves the way for further study of immune phenotypes in FXS patients.

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Homozysteinspiegel von Patienten mit Raynaud-Phänomen

VASA ◽

10.1024/0301-1526.31.2.87 ◽

2002 ◽

Vol 31 (2) ◽

pp. 87-90 ◽

Cited By ~ 7

Author(s):

Al-Awami ◽

Schillinger ◽

Maca ◽

Gschwandtner ◽

Bieglmayer ◽

...

Keyword(s):

Endothelial Cells ◽

Plasma Levels ◽

Serum Levels ◽

Plasma Homocysteine ◽

Serum Folate ◽

Vitamin B ◽

Healthy Controls ◽

Healthy Individuals ◽

Homocysteine Concentration ◽

Age And Sex

Background: Patients with Raynaud’s phenomenon (RP) have vasomotor dysregulation, mainly caused by dysfunction of the endothelium. Since homocysteine has been found to be damaging to endothelial cells, we investigated the concentrations of plasma homocysteine, folate and vitamin B12 in patients with primary or secondary RP compared to healthy individuals. Patients and methods: We measured the concentrations of plasma fasting homocysteine, folate and vitamin B12 in a group of healthy individuals (n = 45) and in patients with primary (n = 26) or secondary RP (n = 42). Results: Median homocysteine levels in healthy controls and in patients with primary RP, secondary RP were 7.9 (IQR 4.1 to 11.8) 9.8 (IQR 5.1 to14.4), and 10.6 (6.0 to15.3) mumol/L, respectively. Patients with primary and secondary RP had significantly higher homocysteine concentration compared to healthy controls (Kruskal Wallis p = 0.01). After matching for age and sex, patients with either primary or secondary RP showed significantly higher homocysteine levels (Wilcoxon p < 0.0001). No significant differences between the three groups were found concerning serum levels of vitamin B12 (p = 0.9 ) and serum folate levels (p = 0.2). Conclusion: These data demonstrate that patients with RP have higher plasma levels of homocysteine. No significant differences in folate and vitamin B12 levels were found between patients with primary RP, secondary RP, and healthy individuals.These data suggest that homocysteine may play a role in RP and may provide new clues in understanding of the vasomotor dysregulation.

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Comprehensive metabolomic profiling of osteosarcoma based on UHPLC-HRMS

Metabolomics ◽

10.1007/s11306-020-01745-4 ◽

2020 ◽

Vol 16 (12) ◽

Author(s):

Dongming Lv ◽

Yutong Zou ◽

Ziliang Zeng ◽

Hao Yao ◽

Shirong Ding ◽

...

Keyword(s):

Lung Metastases ◽

Methionine Sulfoxide ◽

Serum Levels ◽

Guanosine Monophosphate ◽

Metabolic Reprogramming ◽

Healthy Controls ◽

Serum Samples ◽

Multivariate Statistical ◽

Metabolomic Profiling ◽

Proliferation And Metastasis

Abstract Introduction Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. An increasing number of studies have demonstrated that tumor proliferation and metastasis are closely related to complex metabolic reprogramming. However, there are limited data to provide a comprehensive metabolic picture of osteosarcoma. Objectives Our study aims to identify aberrant metabolic pathways and seek potential adjuvant biomarkers for osteosarcoma. Methods Serum samples were collected from 65 osteosarcoma patients and 30 healthy controls. Nontargeted metabolomic profiling was performed by liquid chromatography-mass spectrometry (LC-MS) based on univariate and multivariate statistical analyses. Results The OPLS-DA model analysis identified clear separations among groups. We identified a set of differential metabolites such as higher serum levels of adenosine-5-monophosphate, inosine-5-monophosphate and guanosine monophosphate in primary OS patients compared to healthy controls, and higher serum levels of 5-aminopentanamide, 13(S)-HpOTrE (FA 18:3 + 2O) and methionine sulfoxide in lung metastatic OS patients compared to primary OS patients, revealing aberrant metabolic features during the proliferation and metastasis of osteosarcoma. We found a group of metabolites especially lactic acid and glutamic acid, with AUC values of 0.97 and 0.98, which could serve as potential adjuvant diagnostic biomarkers for primary osteosarcoma, and a panel of 2 metabolites, 5-aminopentanamide and 13(S)-HpOTrE (FA 18:3 + 2O), with an AUC value of 0.92, that had good monitoring ability for lung metastases. Conclusions Our study provides new insight into the aberrant metabolic features of osteosarcoma. The potential biomarkers identified here may have translational significance.

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Serum levels of TIMP-1 and MMP-7 as potential biomarkers in patients with metastatic colorectal cancer

The International Journal of Biological Markers ◽

10.1177/1724600819866202 ◽

2019 ◽

Vol 34 (3) ◽

pp. 292-301 ◽

Cited By ~ 1

Author(s):

Michal Vočka ◽

Daniel Langer ◽

Vladimir Fryba ◽

Jaromir Petrtyl ◽

Tomas Hanus ◽

...

Keyword(s):

Colorectal Cancer ◽

Metastatic Colorectal Cancer ◽

Carcinoembryonic Antigen ◽

Serum Levels ◽

Healthy Controls ◽

Serum Samples ◽

Gastrointestinal Malignancies ◽

Matrix Metalloproteinase 7 ◽

Growth Promoting ◽

Potent Growth

Objective: Tissue inhibitor of metalloproteinases 1 (TIMP-1) and matrix metalloproteinase 7 (MMP-7) were reported to have potent growth promoting activity. Lack of balance between MMPs and TIMPs is an important factor in the development of gastrointestinal malignancies. Methods: We collected serum samples from 97 patients with metastatic colorectal cancer and 79 samples from healthy controls. Serum levels of TIMP-1 and MMP-7 were measured immunochemically and compared with standard tumor markers carcinoembryonic antigen and CA19-9. Results: Serum levels of TIMP-1 and MMP-7 were significantly higher in patients with colorectal cancer compared to healthy controls (both, P < 0.001). TIMP-1 and MMP-7 correlate with the presence of colon involvement (P = 0.001; P = 0.012) and the presence of liver metastases (P = 0.002; P = 0.037), and negatively correlate with pulmonary metastases (P = 0.014; P = 0.005). MMP-7 had similar sensitivity and the same specificity as carcinoembryonic antigen. TIMP-1 and MMP-7 had better sensitivity than CA19-9. TIMP-1 and MMP-7 level correlate with worse outcome (P = 0.002). Conclusion: The results indicate that TIMP-1 and MMP-7 are effective biomarkers in patients with metastatic colorectal cancer with good sensitivity. TIMP-1 and MMP-7 levels strongly correlate with the extent of liver disease and have prognostic value.

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Myristic Acid Serum Levels and Their Significance for Diagnosis of Systemic Inflammatory Response, Sepsis, and Bacteraemia

Journal of Personalized Medicine ◽

10.3390/jpm11040306 ◽

2021 ◽

Vol 11 (4) ◽

pp. 306

Author(s):

Roman Zazula ◽

Michal Moravec ◽

František Pehal ◽

Tomáš Nejtek ◽

Marek Protuš ◽

...

Keyword(s):

Inflammatory Response ◽

Myristic Acid ◽

Close Correlation ◽

Serum Levels ◽

Severe Trauma ◽

Systemic Inflammatory Response ◽

Sepsis Group ◽

Trauma Patients ◽

Healthy Controls ◽

Serum Samples

Myristic acid is identified as a metabolite with the highest diagnostic sensitivity and specificity in the metabolome of patients with bacteraemia. Its significant decrease has been observed in patients with septic shock not responding to treatment. Another study has reported a close correlation of myristic acid levels with the outcome of severe trauma patients. Myristic acid concentrations were investigated in a cohort of septic patients and patients with Systemic Inflammatory Response Syndrome (SIRS) in 5 consecutive days following diagnosis and compared to healthy controls. The study population groups—Sepsis 34, SIRS 31, and Healthy Control 120 patients were included. Serum samples were analyzed using gas chromatography and mass spectrometry. The myristic acid levels in the Sepsis Group and SIRS Group were found to be significantly higher when compared to healthy controls. The serum concentration of myristic acid in septic patients with bacteraemia was higher than in septic patients without bacteraemia. Most patients with sepsis and SIRS had the highest levels of myristic acid within 24 h after an established diagnosis. Myristic acid should be considered as a new candidate marker of severe inflammation and sepsis. A simplified analysis and sufficient body of validated data are necessary steps towards the introduction of this metabolite into routine clinical practice.

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Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients

Journal of Medical Science ◽

10.20883/medical.e33 ◽

2015 ◽

Vol 84 (1) ◽

pp. 34-40

Author(s):

Anna Olewicz-Gawlik ◽

Izabela Korczowska-Łącka ◽

Paweł Hrycaj

Keyword(s):

Rheumatoid Arthritis ◽

Rheumatoid Factor ◽

Acute Phase Proteins ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Serum Concentrations ◽

Serum Samples ◽

Male And Female ◽

Leukocyte Extravasation

Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p < 0.001) and significantly correlated with serum concentrations of rheumatoid factor (RF) and antibodies against cyclic citrullinated peptides (ACPA) (p = 0.006, p = 0.04, respectively). Moreover, we found significant correlations between the serum levels of soluble (s)P- and sE-selectin and ACT-FR (p = 0.008 and p = 0.03, respectively) only in male RA patients.Conclusions. Fucosylation of ACT differs between male and female RA patients and is related to sP- and sE-selectin levels only in men.

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