scholarly journals Antibacterial activity of Piper betle extracts on Helicobacter pylori and identification of potential compounds

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Nguyen Thi Huyen Trang ◽  
Do Thi Thanh Trung ◽  
Nguyen Thi Thanh Thi ◽  
Pham Thi Luong Hang ◽  
Pham Thi Vinh Hoa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Piper Betle ◽  
Gastric Diseases ◽  
Disk Diffusion Assay ◽  
H Pylori

Helicobacter pylori is one of the most common infectious bacteria in the world that causes gastric diseases leading to cancer. The increase of multiple antibiotic resistance rates of H. pylori have been reported worldwide. Thus, development of novel drugs is urgently required. Piper betle has many therapeutic values in traditional medicine. In this study, therefore, we investigated antibacterial activity of P. betle extracts and their fractions against a H. pylori strain isolated in Vietnam. The agar disk diffusion assay showed inhibition zone of ethyl acetate extract and methanol extract from P. betle leaf that of were 46 mm and 32 mm in diameter, respectively. After fractionation of the ethyl acetate extract through silica gel column chromatography, two peaks, PD2 and PD3, out of 12 fractions showed the strongest antibacterial activity. PD2 was sub-fractionated further by re-chromatography on the silica gel column, and subfraction TK12 gave best resolution on LC-MS analysis. Finally, 4 potential compounds, quercetrin, calodenin B, vitexin and plicatipyrone, were identified in TK12 fraction. 

scholarly journals Antibacterial activity of Piper betle extracts on Helicobacter pylori and identification of potential compounds

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Nguyen Thi Huyen Trang ◽  
Do Thi Thanh Trung ◽  
Nguyen Thi Thanh Thi ◽  
Pham Thi Luong Hang ◽  
Pham Thi Vinh Hoa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Piper Betle ◽  
Gastric Diseases ◽  
Disk Diffusion Assay ◽  
H Pylori

Helicobacter pylori is one of the most common infectious bacteria in the world that causes gastric diseases leading to cancer. The increase of multiple antibiotic resistance rates of H. pylori have been reported worldwide. Thus, development of novel drugs is urgently required. Piper betle has many therapeutic values in traditional medicine. In this study, therefore, we investigated antibacterial activity of P. betle extracts and their fractions against a H. pylori strain isolated in Vietnam. The agar disk diffusion assay showed inhibition zone of ethyl acetate extract and methanol extract from P. betle leaf that of were 46 mm and 32 mm in diameter, respectively. After fractionation of the ethyl acetate extract through silica gel column chromatography, two peaks, PD2 and PD3, out of 12 fractions showed the strongest antibacterial activity. PD2 was sub-fractionated further by re-chromatography on the silica gel column, and subfraction TK12 gave best resolution on LC-MS analysis. Finally, 4 potential compounds, quercetrin, calodenin B, vitexin and plicatipyrone, were identified in TK12 fraction. 

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals Bioactive Compounds Isolated from Indonesian Epiphytic Plant of Sarang Semut and Their Antibacterial Activity against Pathogenic Oral Bacteria

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Dikdik Kurnia ◽  
Dadan Sumiarsa ◽  
Hendra D.A. Dharsono ◽  
Mieke H. Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Steroid Glycoside ◽  
Oral Bacteria ◽  
Epiphytic Plant

A new phenolic compound (1), a steroid (2), a new steroid glycoside (4), two triterpenoids (3 & 6) and a new phloroglucinol-sesquiterpene (5) have been isolated from the ethyl acetate extract of Sarang Semut ( Myrmecodia pendans) and their structures were determined on the basis of the spectral data. The bioactivity evaluation was conducted with the inhibition zone of compounds (mm) using Kirby-Bauer method at concentrations of 1000 and 5000 ppm for compound 1 against pathogenic oral bacteria Enterococcus faecalis, was 8.55 and 8.05 mm, respectively. Compounds 2–3 against Streptococcus mutans were 9.00 and 8.45 mm (2) and 10.24 and 9.35 mm (3), respectively. Compound 5 against Porphyromonas gingivalis was 11.5 and 10.8 mm, respectively.

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals UJI EFEKTIVITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.) SEBAGAI ANTIBAKTERI TERHADAP SALMONELLA TYPHI

2019 ◽  
Vol 6 (1) ◽  
pp. 59
Author(s):  
Priska Nancy Claudia Bali ◽  
Ahmad Raif ◽  
Setia Budi Tarigan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Inhibition Zone Diameter ◽  
Negative Controls ◽  
Pandanus Amaryllifolius ◽  
Positive Controls

Pandanus amaryllifolius roxb leaves commonly used as food dditives which contain various compound groups namely alkaloid, saponins, flavonoids and tannins that have a role in antibacterial activity. This research is indicated to support the success of antibacterial in fragrant pandanus leaf plants against Salmonella typhi, one of the pathogenic bacteria. The antibacterial effectiveness test of ethanol-ethyl acetate extract (1: 1) of pandanus leaves which evaluated by the Kirby-bauer method and making the extract concentrations of 25%, 50%, 75%, 100%. The results showed that pandanus leaves extract had antibacterial effectiveness against Salmonella typhi with an average inhibition zone diameter of 11.6 mm; 14 mm; 14.3 mm; 15.3 mm; positive controls (Ciprofloxacin) 27 mm; and negative controls (aquadest) didn’t produce the inhibition zone of Salmonella typhi.

scholarly journals ANTIBACTERIAL ACTIVITY OF FRACTIONS OF ETHYL ACETATE EXTRACT OF GARCINIA LATTISSIMA MIQ. FRUITS

2017 ◽  
Vol 10 (17) ◽  
pp. 81
Author(s):  
Nenengsiti Silfi Ambarwati ◽  
Amarila Malik ◽  
Ageng Tri Listari ◽  
Nirwana Nirwana ◽  
Berna Elya ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Agar Medium ◽  
Bacterial Drug Resistance ◽  
Ethyl Acetate Extracts

Objectives: The emergence of new infections and increase in bacterial drug resistance has created a serious need for the expansion of new antibacterial agents from natural sources. The study was carried out to evaluate the antibacterial activity of fractions of ethyl acetate extract of Garcinia latissima Miq. fruits.Methods: The fractionation was done using a silica gel column and organic solvents as the eluent, i.e., n-hexane, ethyl acetate, and methanol. All fractions were assayed for antibacterial activity, which was done by performing disc diffusion for growth inhibition against Bacillus subtilis and Pseudomonas aeruginosa. In addition, the growth inhibition activity was also examined by performing bioautography assay using pre-coated silica gel 60 GF 254 plates as the stationary phase. Fractions A-F were eluted using n-hexane:chloroform (1:4), while Fractions G-K were used ethyl acetate:dichloromethane (4:1) as the mobile phase. The plate was visualized by ultraviolet at λ 254 nm and 366 nm, while the other one was contacted with the inoculated agar medium to observe zone inhibition. Further, the minimum inhibitory concentration (MIC) value was determined by performing microdilution.Results: The result showed that the antibacterial activity of all fractions was more active at inhibiting the growth of B. subtilis than P. aeruginosa, mainly for Fractions H and J. However, the strongest antibacterial activity was showed by Fractions H and J against B. subtilis, MIC 312.5 μg/mL (lower than reference, which is erythromycin antibiotic (25 μg/mL), followed by Fraction D against B. subtilis MIC 625 μg/mL, Fraction K against P. aeruginosa MIC 625 μg/mL, whereas Fractions C, E, and G against B. subtilis, and Fraction E against P. aeruginosa also showed low MIC values (1.250 μg/mL).Conclusions: The results indicated that fractions of G. latissima Miq. fruit ethyl acetate extracts possessed antibacterial activity. The most active fraction that inhibited the growth of B. subtilis was shown by Fractions H and J; these fractions have the potential to be developed as new antibacterial agents.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Kimia ◽  
2020 ◽  
pp. 56
Author(s):  
N. M. Puspawati ◽  
N. L. P. F. Widiari ◽  
I M. Sukadana
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Spectra Analysis ◽  
Minimum Concentration ◽  
Butanol Extract ◽  
Leaf Powder ◽  
Inhibition Zones

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly.    Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.

scholarly journals LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

2021 ◽  
Author(s):  
Maha A.M. El-Shazly ◽  
Ahmed A. Hamed ◽  
Hoda A. Kabary ◽  
Mosad A. Ghareeb
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Incubation Period ◽  
Phenolic Acids ◽  
Ethyl Acetate Extract ◽  
Wide Spectrum ◽  
Water Extract ◽  
Inhibition Zone ◽  
Dependent Manner ◽  
Prolonged Incubation

Abstract The therapeutical applications of ornamental plants have been categorized to be of a great effectiveness in multiple industries from ancient times until present days. Pluchea dioscoridis is widely known Egyptian wooden plant that has been extensively applied for different medicinal purposes. In this study, LC-ESI-MS/MS analysis of the potent antimicrobial ethyl acetate and n-butanol extracts of P. dioscoridis leaves led to identification of 28 and 21 compounds, respectively. The identified compounds were categorized as phenolic acids, phenolic acids derivatives, organic acids, flavonoids (aglycones and glycosides), secoiridoids, coumarin derivatives, and gallotannins derivatives. Among them, caffeic acid 3-sulfate was the most predominate in the investigated extracts followed by ferulic acid and dicaffeoyl-quinic acid. Also, the antimicrobial potentiality of different extracts was evaluated against different pathogenic microbes including Enterobacter cloacae, Micrococcus leutus, Aeromonas hydrophila, Bacillus subtilis, Bacillus cereus, Bacillus lichneformis and Clostridium species. Furthermore, different concentrations of the most potent extract were assayed for antibacterial efficacy on growth curve kinetics against the susceptible bacteria along 4days incubation period. Our gathered data confirmed that, the antimicrobial activity against tested bacteria was different according to the solvent used in the extraction process. Mostly, all the extracts showed a wide spectrum antibacterial activity except the plant water extract which shows a mild activity against Clostridium sp. only. Based on the highest inhibition zone diameter, the ethyl acetate extract followed by butanol extract exhibited the highest inhibition zone with Micrococcus luteus and B. subtilis (20.0 and 18.5 mm) respectively. Determining the effect of ethyl acetate extract at different concentration (0, 0.66, 1.66, 3.33, 6.67, 13.34 and 20.01 mg mL−1) on M. luteus growth kinetics, the data assured that the antibacterial activity shows concentration dependent manner with the highest antibacterial activity at 20.01 mg mL−1 culture. The data also confirmed that, none of the selected concentration showed bactericidal activity in the prepared cultures, and with the prolonged incubation period the bacteria acquire resistance against the extract beginning from second or third day of incubation.

scholarly journals Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

2020 ◽  
Vol 11 (1) ◽  
pp. 52-60
Author(s):  
Sanusi Ibrahim ◽  
Suryati Suryati ◽  
Enda Desriansyah Aziz
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Cytotoxicity Activity ◽  
E Coli ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Ethyl Acetate Extracts

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK BIJI KAPULAGA (Amomum compactum Sol. Ex Maton)

2016 ◽  
Vol 17 (2) ◽  
pp. 119-129
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Muhamad Zaenudin
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Amomum Compactum

Testing of antibacterial activity against ethyl acetate extract local cardamom seeds (Amomum compactum Sol. Ex Maton) has been performed. Extraction was carried out using the soxhlet method with methanol solvent and liquid-liquid partitioned with n-hexane, ethyl acetate and n-butanol solvent, antibacterial activity test was performed using the disc diffusion method, fractionation using column chromatography and characterization of active fractions using chromatography GCMS, UV-vis and FTIR spectroscopy. The test results showed that the antibacterial activity of ethyl acetate extract had the highest antibacterial activity against S. aureus and E. coli with inhibition zone diameter of respectively 15.15 ± 1.34 and 13.50 ± 0.70 mm at a concentration of 3200 mg/mL. Results of fractionation of the ethyl acetate fraction using column chromatography with a mobile phase of ethyl acetate: n-hexane (3: 2) yielded three fractions, namely F1 (14.6 mg), F2 (8.1 mg) and F3 (4.6 mg). Fraction 2 had the highest antibacterial activity against S. aureus with inhibition zone diameter of 12.34 ± 0.07 mm at a concentration of 800 ug/mL. The results of the characterization of the fraction 2 obtained using GCMS analysis of three antibacterial compounds suspected of 2.9-dihydroxy-1,8-cineol; 2,4-dihydroxy-1,8-cineol and 2,2-methylene bis [6- (1,1-dimethylethyl) -4-ethyl] phenol. The results of the F2 fraction characterization using UV-Vis spectroscopy showed the presence of group C = C conjugated chromophore at λmax 223 nm and are based on analysis using FTIR there -OH alcohol functional group (3372 cm-1), aliphatic -CH (2926 and 2854 cm-1) , C = C (1695 cm-1), aliphatic CH2 (1402 cm-1), CH3 aliphatic (1384 cm-1), and C-O (1203; 1126; 1091 and 1043 cm-1). Keywords: Antibacterial, S. aureus, E. coli, Amomum compactum Sol. Ex Maton, disk diffusion

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