scholarly journals Integrated management of bacterial wilt of ginger incited by Ralstonia solanacearum

2020 ◽  
Vol 15 (2) ◽  
pp. 86-91
Author(s):  
Roop Singh
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Root Length ◽  
Organic Amendment ◽  
Integrated Management ◽  
Shoot Length ◽  
Biocontrol Agents ◽  
Seedling Mortality ◽  
Vigour Index ◽  
Seed Rot

An experiment was conducted to find out the integrated management of Ralstonia solanacearum infecting ginger. Two antibiotics, two biocontrol agents, one phytoextract and one organic amendment and their combination were used. The average reduction in pre emergence seed rot and post emergence seedling mortality recorded with all the treatments tested were ranged from 16.50 to 80.39 per cent over untreated. However, significantly highest reduction in average preemergence seed rot and post- emergence seedling mortality was recorded with streptocycline + P. fluorescens (80.39%). This was followed by streptocycline + karanj cake (72.31%), streptocycline + T. viride (69.55%), streptocycline (58.68%), karanj cake + A. sativum (48.49%), P. fluorescens (44.94%), karanj cake (36.79%). Whereas, T. viride, A. sativum and gentamycin were found least effective with comparatively minimum reduction in average mortality, 26.94, 20.94 and 16.50 per cent, respectively. Of the treatments tested, significantly highest root length (8.60 cm), shoot length (18.43 cm) and vigour index (2342.69) were recorded with streptocycline + P. fluorescens.

scholarly journals Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Growth And Yield ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

scholarly journals Bioconversion of Non Edible Vegetables from Market into Biofertilizer for Crop Improvement

2016 ◽  
Vol 8 (6) ◽  
pp. 71
Author(s):  
Geetha Karuppasamy ◽  
Michael Antony D'Couto ◽  
Anant Achary
Keyword(s):  
Plant Growth ◽  
Root Length ◽  
Growth Parameters ◽  
Crop Improvement ◽  
C:N Ratio ◽  
Value Added ◽  
Fold Increase ◽  
Shoot Length ◽  
Germination Index ◽  
Vigour Index

<p>The fruits and vegetables lost due to spoilage in the market can be converted to nutrient rich organic biofertilizer, vermicompost and worm tea. In this study, non-edible vegetables from market [French beans (S1), Lady’s fingers (S2) and Brinjal (S3)] were used for production of vermicompost and worm tea using <em>Eisenia fetida</em> for environmental friendly management and recycling, as value added product for crop production. Vermicomposting was carried out in four bins. Three bins (S1, S2 &amp; S3) consisted of individual pre-digested vegetables and the fourth bin (S4) was set up with a mixture of all three pre-digested vegetables in equal proportions. Physico-chemical properties and essential nutrients like pH, temperature, moisture content, total organic carbon (C), total nitrogen (N), available phosphorus (P) and exchangeable potassium (K) of the pre-digested vegetables and/or compost were analysed at regular time intervals. There was around 6-10 fold increase in earthworm count at the end of vermicomposting. During vermicomposting, the N and P content of the compost showed 2 to 4 fold increase whereas 10 to 15 fold increase in K content was noticed. Low C:N ratio (4 to 9) was obtained for vermicompost VS4. The worm tea (vermi wash) and vermicompost collected were used for plant growth studies on <em>Vigna radiata</em> (Green gram). Growth parameters like germination percentage, vigour index, germination index (GI), shoot length (cm), root length (cm) and leaf length (cm) were studied. There was noticeable improvement in germination % (1.7 fold), vigour index (2.7 fold) and germination index. A 20 fold increase in shoot length was also seen in test plants when compared to control (2 fold). Statistical analysis of various growth parameters like root length and plant height indicated that vermicompost made with waste brinjal has a significant response with p ≤ 0.05. Based on the results obtained, waste brinjal, which is abundantly available locally can be economically converted to organic biofertilizers and used for soil and crop improvement. Through this study, a cost effective and environment friendly method for efficient utilization of market waste vegetables has been proposed for promoting plant growth and development.</p>

scholarly journals Eco-friendly Management of Bacterial Wilt in Tomato Using Dried Powder of the Wild Arid-Land Medicinal Shrub Rhazya stricta

Plant Disease ◽  
2020 ◽  
Vol 104 (10) ◽  
pp. 2613-2621
Author(s):  
Raja Asad Ali Khan ◽  
Syed Sartaj Alam ◽  
Sania Hayat ◽  
Musharaf Ahmad ◽  
Asad Ali ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Bacterial Wilt ◽  
Root Length ◽  
Shoot Length ◽  
Arid Land ◽  
Effective Control ◽  
Infested Soil ◽  
Fresh Biomass ◽  
Rhazya Stricta

Bacterial wilt (BW) disease caused by Ralstonia solanacearum species complex is a devastating plant disease that inflicts heavy losses to the large number of economic host plants it infects. In this study, the potential of dried powder of the arid-land medicinal shrub Rhazya stricta to control BW of tomato was explored. Both, in vitro and in planta studies were conducted, using different concentrations of dried powder of plant parts, and applied (surface mulched or mixed) to infested soil at 0, 10, and 20 days before transplanting (DBT). Aqueous extract of leaves (16% w/v) was found to be as effective as streptomycin (100 ppm) in inhibiting the in vitro growth of R. solanacearum. As evident from the scanning electron micrograph, 16% aqueous extract of leaves produced severe morphological changes, such as rupture of the bacterial cell walls. Results from the greenhouse experiments demonstrated that the higher powder dose (succulent shoot), namely, 30 g/kg of soil mixed with infested soil 20 DBT, was found to be the most effective in controlling BW. It increased root length (cm), shoot length (cm), and plant fresh biomass (g) by 55, 42, and 40%, respectively, over control plants. Mixing of plant powder with the artificially infested (35 ml of 108 CFU/ml per kilogram of soil) pot soil was better than surface mulching. The 30 g/kg of soil dose mixed with soil increased root length (cm), shoot length (cm), and plant fresh biomass (g) of treated plants by 67, 36, and 46%, respectively, over control plants. A 37% decrease in disease severity over the control was observed with drench application of 30 g of powder per kilogram of soil applied once at 20 DBT. Our results indicated that the dried powder (30 g/kg of soil) of leaves or succulent shoots of R. stricta, thoroughly mixed with soil, 20 DBT, could act as an effective control method against BW.

scholarly journals Fungicidal seed treatment on germination and seedling vigour of lentil var. BINA Masur-3

2017 ◽  
Vol 3 (1) ◽  
pp. 140-144
Author(s):  
A Rokib ◽  
MS Monjil
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Seed Treatment ◽  
Shoot Length ◽  
Seedling Vigour ◽  
Green House ◽  
Seed Pathology ◽  
Vigour Index ◽  
Positive Effect ◽  
Improve Seed Germination

Effectiveness of six fungicides viz., Provax-200 (Carboxin+Thiram), Bavistin DF (Carbendazim), Dithane M-45 (Mancozeb), Secure (Mancozeb+Fenamidone), Antracol (propineb) and Daconil (Chlorothalonil) were evaluated to improve seed germination and seedling vigour of lentil variety BINA Masur-3. The experiment was conducted in the Green House of Seed Pathology Centre, Bangladesh Agricultural University, Mymensingh. Positive effect of seed treatment with fungicides on seed germination and seedling vigour of lentil was recorded. At 20 DAS, increased seed germination over control was observed in treated seeds with Deconil (10.40%) and Antracol (10.00%) followed by Dithane M-45 (7.20%). Seeds treated with Deconil and Dithane M-45 produced seedlings with higher shoot length, root length and seedling vigour. At 20 DAS, higher percent increased vigour index over control was found in Dithane M-45 (24.64%) and Deconil (22.44%), respectively.Asian J. Med. Biol. Res. March 2017, 3(1): 140-144

scholarly journals Seed quality enhancement through biopriming in common bean (Phaseolus vulgaris. L)

2017 ◽  
Vol 9 (3) ◽  
pp. 1740-1743
Author(s):  
S. P. Monalisa ◽  
J. K. Beura ◽  
R. K. Tarai ◽  
M. Naik
Keyword(s):  
Phaseolus Vulgaris ◽  
Trichoderma Harzianum ◽  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Shoot Length ◽  
Quality Enhancement ◽  
Phaseolus Vulgaris L ◽  
Seedling Vigour ◽  
Vigour Index

An experiment on seed quality enhancement of common bean (Phaseolus vulgaris L.) var. S 9 (local) was conducted at the department of seed science and technology, OUAT, Bhubaneswar during 2013-14 by use of three biocontrol agents viz. Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescence. Seeds were bi-oprimed with the biocontrol agents at 40, 50 and 60 % concentration for 4,8,12 and 16 hours of soaking. Seeds were also hydro primed for 4,8,12 and 16 hours. Unprimed dry seed resulted in germination (69 %), shoot length (27.5 cm), root length (14 cm), seedling dry weight (1.71g), SVI-I (2859.2), SVI-II (118.0) and speed of germination (5.8) while hydro primed seeds resulted in germination (72%), shoot length (31.9 cm), root length (15 cm), seedling dry weight (1.80 g), SVI-1 (3375.9) SVI-II (129.8) and speed of germination (6.7). Trichoderma harzianum at 40% con-centration and for 4 hours of soaking resulted enhancement of above quality parameter like 13.0 % in germination, 21.1 % in shoot length, 20.7 % in root length, 31.6 % in seedling dry weight, 36 % in seedling vigour index-I, 48.1 % in seedling vigour index-II and 58.6 % in speed of germination over unprimed seeds. Bio priming with P. fluorescence ( at 40% concentration and for 4 hour) closely followed and at par with best treatment with 11.6 %, 18.2 %, 16.4 %, 30.4 %, 30.7 % and 56.9 % enhancement of above mentioned quality parameters, respectively.

scholarly journals Evaluation of tomato rootstocks to Ralstonia solanacearum and R. pseudosolanacearum in Mata mesoregion, PE

2021 ◽  
Vol 39 (1) ◽  
pp. 72-78
Author(s):  
Géssyka R Albuquerque ◽  
Lucas P Lucena ◽  
Emanuel F Assunção ◽  
Júlio Carlos P Mesquita ◽  
Adriano Márcio F Silva ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Integrated Management ◽  
Block Design ◽  
Bacterial Strains ◽  
Tomato Production ◽  
Randomized Block Design ◽  
Randomized Experimental Design ◽  
Production Area ◽  
Tomato Genotype

ABSTRACT Bacterial wilt limits tomato production and resistant rootstocks could be important for the integrated management of the disease. Since there is an interaction between local bacterial strains and tomato genotype, this study aimed to evaluate 14 tomato rootstocks to bacterial wilt in the Mata mesoregion of Pernambuco state, Brazil. The rootstocks reaction to two sequevars of Ralstonia solanacearum and two of R. pseudosolanacearum was evaluated in four experiments carried out in the greenhouse using the completely randomized experimental design, with four replications composed of four plants each. Seven genotypes were selected to evaluate the reaction to bacterial wilt as rootstocks grafting in tomato plants ‘Tomini F1’ in a production area with disease history in the Chã Grande municipality, using randomized block design with four plants per treatment in each block. In the field experiment, disease symptoms were not observed in the grafted plants in ‘Guardião’, ‘Woodstock’, and ‘Yoshimatsu’. Regarding all experiments, ‘Guardião’ and ‘Muralha’ showed the best resistance levels and could be used in the integrated management of bacterial wilt and studied in plant breeding programs.

scholarly journals Effect of nitrogen and plant growth regulators on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.)

2017 ◽  
Vol 9 (4) ◽  
pp. 2338-2343
Author(s):  
Arpana D. Vaja ◽  
J. B. Patel ◽  
R.N. Daki ◽  
Shital A. Chauhan
Keyword(s):  
Seed Yield ◽  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Vigour Index

The present investigation on effect of nitrogen [N0 (Control), N1 (50 kg N/ha), N2 (100 kg N/ha), and N3 (150 kg N/ ha)] and plant growth regulators [G0 (Control), G1 (50 ppm GA3), G2 (50 ppm NAA) and G3 (500 ppm Cycocel)] on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.) cv. GJB 3 was carried out at the Instructional Farm, Department of Agronomy, Junagadh Agricultural University, Junagadh during kharif 2015-16. The experiment was laid out in field as per randomized block design (Factorial) with three replications. The seed harvested from 16 different treatments combinations replicated thrice from the field were analyzed in the laboratory following completely randomized design (factorial) for various seed quality parameters. Application of nitrogen @ 150 kg N/ha recorded significantly (P<0.05) highest seed yield per plant, shoot fresh weight, root dry weight, shoot dry weight and vigour index – 2 (mass), while application of 100 kg N/ha resulted in significantly (P<0.05) highest germination percentage, root length, shoot length, root fresh weight and vigour index – 1 (length). Application of GA3 at 50 ppm recorded significantly the highest seed yield per plant, germination percentage, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, vigour index – 1 (length) and vigour index – 2 (mass). Among the 16 different treatment combinations, nitrogen @ 150 kg/ha and GA3 50 ppm noted the maximum seed yield per plant, shoot dry weight and vigour index – 2 (mass), while significantly the maxi-mum germination percentage, root length, shoot length, root fresh weight, shoot fresh weight and vigour index – 1 (length) were registered in treatment combination nitrogen 100 kg N/ha and GA3 @ 50 ppm. Therefore, it is advised that application of 100-150 kg of N/ha as a nitrogenous fertilizer and spray GA3 @ 50 ppm (G1) at 45 days after transplanting helps in increasing the seed yield per plant and seed quality parameters in brinjal seed production.

Inhibitory Effect of Aqueous Extracts of Tree-legumes on Germination and Seedling Growth of Food Legume, Green Gram (Vigna radiata L.)

2021 ◽  
Author(s):  
Anbarasu Mariyappillai ◽  
Swaminathan Chitraputhira Pillai
Keyword(s):  
Root Length ◽  
Vigna Radiata ◽  
Shoot Length ◽  
Green Gram ◽  
Allelopathic Effect ◽  
Aqueous Extracts ◽  
Seedling Vigour ◽  
Delonix Regia ◽  
Plant Parts ◽  
Vigour Index

Background: Green gram (Vigna radiata), the third most important pulse crop, is grown in tank-fed, command areas after the harvest of paddy. In such areas, legume trees like Delonix regia and Peltophorum pterocarpum are commonly found and tree parts often fell in watercourses and were flown to crop fields along with irrigation water. Hence, it is necessary to study whether any allelopathic effect is present in these parts of trees which may affect the growth and development of green gram grown in farmland crops. Methods: Collected different tree parts viz., bark, leaf and fresh flower, a matured pod of Delonix regia and Peltophorum pterocarpum, cleaned and soaked distilled water at weight/volume ratio of 1:10 for 24 hrs. Aqueous extracts were filtered and tested for inhibition of green gram. Pot culture experiments were conducted with 10 treatments and four replications. Germination (%), shoot length (cm), root length (cm), fresh weight (mg), dry weight (mg), seedling vigour index-I and vigour index-II in green gram were recorded and the magnitude of inhibition versus simulation in bioassay was compared through response index.Result: Among the plant parts, Delonix regia flower and Peltophorum pterocarpum matured pods suppressed germination (45.00 and 60.00%), shoot length (13.87 and 13.39 cm), root length (4.27 and 10.49 cm), seedling vigour index-I (624.15 and 803.40) and seedling vigour index-II (960.75 and 948.00) respectively in green gram. This might due to presence of inhibitory chemicals in flowers of Delonix regia and matured pods of Peltophorum pterocarpum compared to other plant parts of trees.

scholarly journals An improved control efficacy against tobacco bacterial wilt by an engineered Pseudomonas mosselii expressing the ripAA gene from phytopathogenic Ralstonia solanacearum

2019 ◽  
Author(s):  
Tao Zhuo ◽  
Shiting Chen ◽  
Xiaojing Fan ◽  
Xun Hu ◽  
Huasong Zou
Keyword(s):  
Plant Pathogen ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biocontrol Agent ◽  
Biocontrol Agents ◽  
Avirulence Gene ◽  
Primary Immune Response ◽  
Systemic Resistance ◽  
Tobacco Plants ◽  
Engineered Strain

AbstractThe environmental bacterium Pseudomonas mosselii produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including Ralstonia solanacearum, the causal agent of bacterial wilt. In this study, an engineered P. mosselii strain was generated to express R. solanacearum ripAA, which determines incompatible interactions with tobacco plants. The ripAA gene together with its native promoter was integrated into the P. mosselii chromosome. The resulting strain showed no difference in antimicrobial activity against R. solanacearum. Promoter-LacZ fusion and RT-PCR experiments demonstrated that the ripAA gene was transcribed in culture media. Compared with that of the wild type, the engineered strain reduced the disease index by 9.1% for bacterial wilt on tobacco plants. A transcriptome analysis was performed to identify differentially expressed genes in tobacco plants, and the results revealed that ethylene-and jasmonate-dependent defense signaling pathways were induced. These data demonstrated that the engineered P. mosselii expressing ripAA enables improved biological control against tobacco bacterial wilt by the activation of host defense responses.ImportanceNowadays, the use of biocontrol agents is more and more popular in agriculture, but they cannot replaced of chemical agents mostly, due to the poorer control effect. So the study about how to improve the efficacy of biocontrol agents become necessary and urgent. We increase the efficacy against plant pathogen through introducing an avirulence gene from plant pathogen into the biocontrol agent based on “gene to gene” hypothesis. The new engineered strain can improve the systemic resistance and elicit primary immune response of plants. Our research not only provides a new strategy for genetic modification of biocontrol agent, a number of avirulence gene from pathogen or plant can be tested to be expressed in different biocontrol agents to antagonize plant disease, but also help the study of interaction between phythopathogenic avirulence gene and host.

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