scholarly journals Evaluation of tomato rootstocks to Ralstonia solanacearum and R. pseudosolanacearum in Mata mesoregion, PE

2021 ◽  
Vol 39 (1) ◽  
pp. 72-78
Author(s):  
Géssyka R Albuquerque ◽  
Lucas P Lucena ◽  
Emanuel F Assunção ◽  
Júlio Carlos P Mesquita ◽  
Adriano Márcio F Silva ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Integrated Management ◽  
Block Design ◽  
Bacterial Strains ◽  
Tomato Production ◽  
Randomized Block Design ◽  
Randomized Experimental Design ◽  
Production Area ◽  
Tomato Genotype

ABSTRACT Bacterial wilt limits tomato production and resistant rootstocks could be important for the integrated management of the disease. Since there is an interaction between local bacterial strains and tomato genotype, this study aimed to evaluate 14 tomato rootstocks to bacterial wilt in the Mata mesoregion of Pernambuco state, Brazil. The rootstocks reaction to two sequevars of Ralstonia solanacearum and two of R. pseudosolanacearum was evaluated in four experiments carried out in the greenhouse using the completely randomized experimental design, with four replications composed of four plants each. Seven genotypes were selected to evaluate the reaction to bacterial wilt as rootstocks grafting in tomato plants ‘Tomini F1’ in a production area with disease history in the Chã Grande municipality, using randomized block design with four plants per treatment in each block. In the field experiment, disease symptoms were not observed in the grafted plants in ‘Guardião’, ‘Woodstock’, and ‘Yoshimatsu’. Regarding all experiments, ‘Guardião’ and ‘Muralha’ showed the best resistance levels and could be used in the integrated management of bacterial wilt and studied in plant breeding programs.

Compatibilidade de enxertia de híbridos interespecíficos de Solanum com tomateiro visando controle de patógenos de solo / Grafting compatibility of interspecific Solanum hybrids with tomato to control soilborne plant pathogens

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
José Lindorico de Mendonça ◽  
Carlos Alberto Lopes ◽  
Antonio Willians Moita
Keyword(s):  
Bacterial Wilt ◽  
Interspecific Hybrids ◽  
Plant Pathogens ◽  
Block Design ◽  
Fruit Weight ◽  
Small Scale ◽  
Tomato Cultivar ◽  
Tomato Production ◽  
Randomized Block Design ◽  
Grafting Technique

Em condições de alta temperatura e umidade e na presença de solos contaminados com patógenos de solo, situações comuns em cultivo protegido e em campo aberto em algumas regiões do Brasil, produzir tomate é um grande desafio. Na década de 1950, o controle da doença murcha bacteriana, por exemplo, foi conseguido utilizando-se como porta-enxerto a jurubeba ‘Juna’ (Solanum stramonifolium) em enxerto de tomateiro comercial. Embora possibilite um bom nível de controle, o uso de espécies de jurubeba do subgênero Leptostemanum tem o inconveniente de possuir acúleos. Esta característica foi determinante no abandono da técnica da enxertia, pois comprometia consideravelmente o rendimento de trabalho ao ferir as mãos do enxertador. O objetivo deste trabalho foi avaliar a compatibilidade de híbridos interespecíficos de Solanum resistentes a patógenos de solo como porta-enxerto de tomateiro. Três híbridos interespecíficos: jiló ‘CNPH 221’ (S. aethiopicum) X jurubeba Juna ‘CNPH 19’ (S. stramonifolium); berinjela ‘CNPH 171’(S. melongena) X jurubeba Juna ‘CNPH 19’ (S. stramonifolium); jiló ‘CNPH 222’ (S. aethiopicum) X berinjela ‘Ciça’ (S. melongena);  e os acessos: jurubeba Juna ‘CNPH 19’; berinjela ‘CNPH 171’ e o porta-enxerto comercial de tomateiro ‘Guardião’ (Takii), foram avaliados como porta-enxerto do tomateiro comercial ‘Ellen’ (Feltrin) usado como enxerto, além de ‘Ellen’ auto-enxertado. O experimento foi conduzido em casa de vegetação na Embrapa Hortaliças, em vasos com 8 litros de solo, e o método de enxertia usado foi o de fenda simples. O delineamento experimental foi inteiramente casualizado com três repetições e oito plantas por parcela. As plantas foram submetidas aos tratos culturais normais da cultura em ambiente protegido. Foram avaliados a massa de frutos comerciais, total e refugo, e número total de frutos. As combinações com os híbridos interespecíficos não diferiram estatisticamente de ‘Ellen’ auto–enxertada para as variáveis avaliadas, indicando que estes porta-enxertos tem boa compatibilidade de enxertia com esta cultivar, além de não possuírem acúleos. Entre porta-enxertos simples, jurubeba juna ‘CNPH 19’ superou a berinjela ‘CNPH 171’ e não diferiu dos demais tratamentos.    ABSTRACT Under warm temperatures, high soil humidity, and in the presence of soilborne pathogens, conditions commonly found in protected crops and in some regions in Brazil, soil-associated diseases are a constraint to tomato production all year round. In the 1950’s, bacterial wilt control, for example, was achieved in small scale by grafting tomato onto jurubeba juna (Solanum stramonifolium). Even though the level of bacterial wilt protection was adequate with this combination, the grafting technique was halted, since species of jurubeba of the subgenus Leptostemanum is very spiny, a characteristic that made the hand operation unpleasant and slow. The objective of this work was to test some spineless hybrids, previously screened for resistance to bacterial wilt, for their compatibility with tomato. Three interspecific hybrids: S. aethiopicum X S. stramonifolium; S. melongena X S. stramonifolium, and S. aethiopicum X S. melongena were tested as rootstocks for commercial cultivar Ellen, in comparison with self-grafted ‘Ellen’, as well as grafted on S. stramonifollium ‘CNPH 19’ and on S. melongena ‘CNPH 171’, resistant to bacterial wilt, and on commercial tomato rootstock ‘Guardião’. The experiment was carried out with cleft-grafted plants at the 5-leaf stage grown in 8-L pots in a greenhouse at Embrapa Vegetables, Brasilia, DF. The plants were disposed in a complete randomized block design, with plots made of eight plants in three replications. The crop was grown as commercially indicated for protected cultivation. The variables evaluated were total fruit weight, commercial fruit weight, and total fruit number. The combinations with the interspecific hybrids did not differ from self-grafted ‘Ellen’ for all the variables, indicating that they display good compatibility with this specific tomato cultivar. Among the self-pollinated rootstocks, S. stramonifolium was better than eggplant ‘CNPH 171’ in commercial fruit weight, but did not differ from the other treatments.

Distribution and incidence of tomato bacterial wilt caused by Ralstonia solanacearum in the central region of Togo

2021 ◽  
Author(s):  
Bitang Bamazi ◽  
Agnassim Banito ◽  
K. D. Ayisah ◽  
Rachidatou Sikirou ◽  
Mathews Paret ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Laboratory Investigation ◽  
Central Region ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Yield Losses ◽  
Tomato Production ◽  
Solanum Lycopersicum L ◽  
Survey Results ◽  
Tomato Bacterial Wilt

Tomato (Solanum lycopersicum L.) is one of the most important vegetables in Togo. Unfortunately, tomatoes are susceptible to many diseases, among which bacterial wilt caused by Ralstonia solanacearum causes major yield losses. In this study, incidence of bacterial wilt and its distribution was evaluated in the central region of Togo, the major tomato producing area in the country. Overall, 16 localities were surveyed in four prefectures. In each locality, three fields were visited, and the incidence of the disease was recorded, and diseased samples were collected for laboratory investigation. The results showed that bacterial wilt occurred in all the fields visited, indicating a field incidence of 100%, whereas the plant incidence ranged from 10.00±00% to 43.33±3.33%, with an average of 20.94±1.77%. The antibody based Immunostrip test was positive for R. solanacearum in 100% of the visited fields. From 144 samples collected from fields, 45 R. solanacearum isolates were isolated on Modified SMSA media. This survey results show that tomato bacterial wilt is a real threat to tomato production in the central region of Togo.

scholarly journals Genetic Diversity and Aggressiveness of Ralstonia solanacearum Strains Causing Bacterial Wilt of Potato in Uruguay

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1292-1301 ◽  
Author(s):  
M. I. Siri ◽  
A. Sanabria ◽  
M. J. Pianzzola
Keyword(s):  
Genetic Diversity ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Potato Tubers ◽  
Bacterial Strains ◽  
Potato Plants ◽  
Polymerase Chain ◽  
First Time ◽  
Different Levels

Bacterial wilt, caused by Ralstonia solanacearum, is a major disease affecting potato (Solanum tuberosum) production worldwide. Although local reports suggest that the disease is widespread in Uruguay, characterization of prevalent R. solanacearum strains in that country has not been done. In all, 28 strains of R. solanacearum isolated from major potato-growing areas in Uruguay were evaluated, including 26 strains isolated from potato tubers and 2 from soil samples. All strains belonged to phylotype IIB, sequevar 1 (race 3, biovar 2). Genetic diversity of strains was assessed by repetitive-sequence polymerase chain reaction, which showed that the Uruguayan strains constituted a homogeneous group. In contrast, inoculation of the strains on tomato and potato plants showed, for the first time, different levels of aggressiveness among R. solanacearum strains belonging to phylotype IIB, sequevar 1. Aggressiveness assays were also performed on accessions of S. commersonii, a wild species native to Uruguay that is a source of resistance for potato breeding. No significant interactions were found between bacterial strains and potato and S. commersonii genotypes, and differences in aggressiveness among R. solanacearum strains were consistent with previously identified groups based on tomato and potato inoculations. Moreover, variation in responses to R. solanacearum was observed among the S. commersonii accessions tested.

scholarly journals KETAHANAN DELAPAN KULTIVAR TEMBAKAU LOKAL BONDOWOSO TERHADAP TIGA PATOGEN PENTING (Ralstonia solanacearum, Pectobacterium carotovorum, DAN Phytophthora nicotianae)

2020 ◽  
Vol 18 (3) ◽  
pp. 89
Author(s):  
TITIEK YULIANTI ◽  
NURUL HIDAYAH ◽  
SRI YULAIKAH
Keyword(s):  
Ralstonia Solanacearum ◽  
Block Design ◽  
Production Quality ◽  
Phytophthora Nicotianae ◽  
Pectobacterium Carotovorum ◽  
Resistance Level ◽  
Stalk Rot ◽  
Randomized Block Design ◽  
Tobacco Production ◽  
Screen House

<p>ABSTRAK<br />Tembakau bondowoso merupakan tembakau lokal rajangan yang<br />berkembang di Kabupaten Bondowoso, Jawa Timur. Saat ini ada delapan<br />kultivar dengan karakter produksi, mutu, dan ketahanannya terhadap<br />penyakit yang berbeda. Layu bakteri (Ralstonia solanacearum), busuk<br />batang berlubang (Pectobacterium carotovorum), dan lanas (Phytophthora<br />nicotianae) merupakan penyakit yang sering menyebabkan turunnya<br />produksi tembakau bondowoso. Evaluasi ketahanan delapan kultivar<br />tembakau bondowoso (Samporis, Serumpung, Marakot, Samporis Lokal,<br />Samporis AH, Samporis CH, Samporis B. Disbun, dan Deli) terhadap<br />ketiga patogen tersebut dilaksanakan di laboratorium dan rumah kasa Balai<br />Penelitian Tanaman Pemanis dan Serat (Balittas) mulai bulan April sampai<br />dengan Oktober 2011. Penelitian terhadap ketiga patogen tersebut<br />dilakukan secara terpisah. Masing-masing kultivar ditanam sebanyak 10<br />tanaman, 1 tanaman/polibag. Setiap perlakuan (kultivar) diulang 3 kali dan<br />disusun dalam rancangan acak kelompok (RAK). Inokulasi R.<br />solanacearum dan P. carotovorum dilakukan secara terpisah 24 jam<br />sebelum transplanting. Inokulasi P. nicotianae dilakukan dengan dua cara,<br />yaitu melalui akar dan pangkal batang. Inokulasi akar sama dengan cara<br />inokulasi bakteri. Inokulasi pangkal batang dilakukan pada tanaman<br />berumur 2 minggu setelah transplanting. Pengamatan intensitas penyakit<br />dilakukan setiap minggu selama 11 minggu. Hasil penelitian menunjukkan<br />bahwa kultivar Samporis CH, Samporis, dan Deli tahan terhadap P.<br />carotovorum, R. solanacearum, dan P. nicotianae. Kultivar Samporis CH.,<br />Samporis, dan Deli ketahanannya lebih tinggi terhadap ketiga patogen,<br />dengan intensitas penyakit berkisar antara 3,3%-6,7%. Kultivar Marakot<br />sangat rentan terhadap ketiga patogen tersebut dengan tingkat keparahan ≥<br />50%. Demikian pula kultivar Samporis AH yang rentan terhadap R.<br />solanacearum, P. nicotianae dan P. carotovorum dengan intensitas<br />penyakit 23,3-53,3%. Oleh karena itu, kultivar Samporis CH, Samporis,<br />dan Deli cocok dikembangkan pada lahan endemik penyakit tular tanah di<br />Kabupaten Bondowoso.<br />Kata kunci: tembakau  bondowoso, Pectobacterium  carotovorum<br />Phytophthora  nicotianae, Ralstonia  solanacearum,<br />ketahanan</p><p>ABSTRACT<br />Bondowoso tobacco is a local type of sliced tobacco which is<br />restrictedly cultivated in Bondowoso Regency, East Java. There are eight<br />cultivars known, ie. Samporis, Serumpung, Marakot, Samporis Lokal,<br />Samporis AH, Samporis CH, Samporis B. Disbun, and Deli with their own<br />distinctive characters on their production, quality, and resistance to<br />diseases. Bacterial wilt (Ralstonia solanacearum), hollow stalk rot<br />(Pectobacterium carotovorum), and blackshank (Phytophthora nicotianae<br />are the main cause of bondowoso tobacco production loss. Evaluation on<br />the resistance level of the cultivars to the three pathogens above has been<br />conducted at a laboratory and screen house scale in Indonesian Sweetener<br />and Fibre Crops Research Institute from April to October 2011. The<br />evaluation of each pathogen was conducted separately. Each evaluation of<br />the pathogen per cultivar used 10 plants planted individually in a polybag.<br />The experiment was arranged in a randomized block design with 3<br />replicates. R. solanacearum and P. carotovorum were separately<br />inoculated on the test plants 24 h before transplanting. The inoculation of<br />P. nicotianae was done twice via the root and stem. Disease intensity was<br />observed weekly for 11 weeks. The results showed that Samporis CH,<br />Samporis, and Deli cultivars were resistant to P. carotovorum, R.<br />solanacearum and P. nicotianae, whereas Samporis and Deli cultivars<br />were more resistant to the pathogens (disease intensity ranged 3.3-6.7%).<br />Marakot cultivar was very susceptible to all of the three pathogens (disease<br />intensity ≥ 50%). Similarly, Samporis AH cultivar was also susceptible to<br />the pathogens with disease intensity ranged 23.3-53.3%. The study<br />indicated that Samporis CH, Samporis, and Deli cultivars are suitable to be<br />cultivated in the endemic soil born pathogen areas of Bondowoso<br />Regency.<br />Key words: bondowoso  tobacco, Pectobacterium  carotovorum,<br />Phytophthora  nicotianae, Ralstonia  solanacearum,<br />resistance</p>

scholarly journals Tomato grafting onto Solanaceae genotypes to control bacterial wilt (Ralstonia solanacearum Smith 1896)

2020 ◽  
Vol 50 ◽  
Author(s):  
Lívia Tálita da Silva Carvalho ◽  
Danilo Mesquita Melo ◽  
Pablo Forlan Vargas ◽  
Helane Cristina Aguiar Santos ◽  
Jessica Vasconcelos Ferreira
Keyword(s):  
Survival Rate ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Fruit Yield ◽  
Productive Performance ◽  
Preventive Control ◽  
Tomato Production ◽  
Natural Infestation ◽  
Grafting Onto

ABSTRACT The tomato cultivation in the Amazon is not feasible mainly due to the natural infestation of Ralstonia solanacearum in the soil. Preventive control through grafting has been one of the main alternatives to manage this problem. This study aimed to evaluate the compatibility of Solanaceae genotypes as rootstocks for 'Santa Clara' tomato, aiming to control the bacterial wilt. Five rootstocks were evaluated: cubiu; red jurubeba; jurubebão; commercial hybrid tomato rootstock Guardião; and 'Santa Clara' tomato (self-grafting). In the seedling phase, characteristics of rootstock survival rate and compatibility were evaluated; while, in the field, that happened for survival rate, compatibility and fruit yield. In the seedling phase, Guardião and red jurubeba showed the best performances. In the field, red jurubeba presented a low compatibility, although it obtained the best productive performance among the wild rootstocks. Guardião obtained the best compatibility and yield among the studied rootstocks. Grafting is a viable technique for tomato production under soil-borne disease conditions.

scholarly journals Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam pengendalian hayati Ralstonia solanacearum penyebab penyakit layu bakteri pada tomat

Jurnal Agro ◽  
10.15575/2305 ◽  
2018 ◽  
Vol 5 (1) ◽  
pp. 1-12
Author(s):  
Istiqomah Istiqomah ◽  
Dian Eka Kusumawati
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Total Phenol ◽  
Tomato Production ◽  
Bacterial Wilt Disease ◽  
Type Test ◽  
Important Disease

Salah satu penyakit penting pada produksi tomat di Indonesia adalah layu bakteri yang disebabkan oleh Ralstonia solanacearum. Alternatif untuk mengendalikan penyakit layu bakteri adalah dengan menggunakan Bacillus subtilis dan Pseudomonas fluorescens. Tujuan penelitian ini untuk mengetahui kemampuan B. subtilis dan P. fluorescens dalam mengendalikan penyakit layu bakteri yang disebabkan R. solanacearum serta mekanisme penghambatannya. Penelitian ini terdiri dari 5 tahap, yaitu perbanyakan inokulum R. solanacearum, uji virulensi dan uji hipersensitif  R. solanacearum, uji antagonis B. subtilis dan P. fluorescens terhadap R. solanacearum pada media agar, uji jenis antibiosis, penelitian di rumah kaca, dan analisis total fenol. Hasil penelitian uji antagonis menunjukkan bahwa semua isolat B. subtilis dan P. fluorescens memiliki potensi menghambat R. solanacearum dengan tipe antibiosis bakteriostatik. Hasil analisis kadar fenol menunjukkan bahwa terjadi peningkatan total fenol secara signifikan pada tanaman tomat yang diaplikasikan isolat B. subtilis UB-ABS6, P. fluorescens UB-PF5 dan P. fluorescens UB-PF6. Penelitian di rumah kaca menunjukkan bahwa semua tanaman tomat yang diaplikasikan agens hayati mengalami penundaan masa inkubasi dibandingkan dengan kontrol. Isolat B. subtilis UB-ABS2, B. subtilis UB-ABS6, P. fluorescens UB-PF5 dan P. fluorescens UB-PF6 secara signifikan menekan kejadian penyakit layu bakteri berturut-turut 50%, 30%, 60%, dan 60%. B. subtilis dan P. fluorescens dapat dimanfaatkan untuk mengendalikan layu bakteri pada tomat yang disebabkan oleh Ralstonia solanacearum. One of important disease that infects tomato production in Indonesia is bacterial wilt disease caused by Ralstonia solanacearum. Alternative on controlling bacterial wilt is using Bacillus subtilis and Pseudomonas fluorescens. Goal of the research was to find out ability of B. subtilis and P. fluorescens to control R. Solanacearum and mechanism of the inhibition. This research divided into 5 stages, i.e. propagation of R. solanacearum, virulence and hypersensitive tests of R. Solanacearum, antagonist test of B. subtilis and P. fluorescens against R. solanacearum on agar medium, antibiosis type test, research in greenhouse, and total phenol analysis. The result showed that all isolates of B. subtilis and P. fluorescens have potential to inhibite R. solanacearum by bacteriostatic antibiosis type. The total phenol level showed significant increase of phenol on tomato along with the application of isolates B. subtilis UB-ABS6, P. fluorescens UB-PF5 and P. fluorescens UB-PF6. Research in the greenhouse showed that all tomatoes, which had been given bioagent, did delay on the incubation than the control. Isolates of B. subtilis UB-ABS2, B. subtilis UB-ABS6, P. fluorescens UB-PF5, and P. fluorescens UB-PF6 had significantly inhibited the bacterial wilt disease 50%, 30%, 60%, and 60%, respectively. Therefore, B. subtilis and P. fluorescens can be used to control bacterial wilt diseases on tomato caused by Ralstonia solanacearum.

scholarly journals Growth promotion of Guinea grass by diazotrophic bacteria1

2019 ◽  
Vol 49 ◽  
Author(s):  
Konrad Passos Silva ◽  
Gian Otávio Alves Silva ◽  
Tayla Évellin Oliveira ◽  
Adauton Vilela Rezende ◽  
Ligiane Aparecida Florentino
Keyword(s):  
Growth Promotion ◽  
Block Design ◽  
Dry Mass ◽  
Stem Diameter ◽  
Panicum Maximum ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Randomized Block Design ◽  
Nitrogen Inputs ◽  
Guinea Grass

ABSTRACT The Panicum maximum species (cv. Mombaça) is a forage plant well adapted to the different edaphoclimatic conditions found in Brazil; however, it requires high nitrogen fertilizer doses to ensure high yields. The present study aimed to assess the inoculation effect with associative diazotrophic bacteria strains on the yield and bromatological characteristics of the Guinea grass. A randomized block design was used, consisting of 25 treatments, being 23 of them inoculated with diazotrophic bacteria and two without inoculation (one with and the other without mineral nitrogen), with four repetitions. The analyzed variables were stem diameter; leaf width and length; leaf, stem and root dry mass yield; crude protein; acid and neutral detergent fibers. There were no differences among the treatments for root dry mass, stem diameter and acid detergent fiber. For the remaining variables, a positive effect of the inoculation with diazotrophic bacteria was observed, particularly for the following Unifenas strains: 100-06, 100-13, 100-26, 100-30, 10-35, 100-54, 100-69, 100-71 and 100-94. This reveals the potential of these bacterial strains for use at the sustainable production of the Guinea grass with a reduction in the use of nitrogen inputs.

scholarly journals Integrated management of bacterial wilt of ginger incited by Ralstonia solanacearum

2020 ◽  
Vol 15 (2) ◽  
pp. 86-91
Author(s):  
Roop Singh
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Root Length ◽  
Organic Amendment ◽  
Integrated Management ◽  
Shoot Length ◽  
Biocontrol Agents ◽  
Seedling Mortality ◽  
Vigour Index ◽  
Seed Rot

An experiment was conducted to find out the integrated management of Ralstonia solanacearum infecting ginger. Two antibiotics, two biocontrol agents, one phytoextract and one organic amendment and their combination were used. The average reduction in pre emergence seed rot and post emergence seedling mortality recorded with all the treatments tested were ranged from 16.50 to 80.39 per cent over untreated. However, significantly highest reduction in average preemergence seed rot and post- emergence seedling mortality was recorded with streptocycline + P. fluorescens (80.39%). This was followed by streptocycline + karanj cake (72.31%), streptocycline + T. viride (69.55%), streptocycline (58.68%), karanj cake + A. sativum (48.49%), P. fluorescens (44.94%), karanj cake (36.79%). Whereas, T. viride, A. sativum and gentamycin were found least effective with comparatively minimum reduction in average mortality, 26.94, 20.94 and 16.50 per cent, respectively. Of the treatments tested, significantly highest root length (8.60 cm), shoot length (18.43 cm) and vigour index (2342.69) were recorded with streptocycline + P. fluorescens.

scholarly journals Combined use of a microbial restoration substrate and avirulent Ralstonia solanacearum for the control of tomato bacterial wilt

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xuefang Zheng ◽  
Yujing Zhu ◽  
Jieping Wang ◽  
Ziran Wang ◽  
Bo Liu
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Combined Treatment ◽  
Control Measures ◽  
Effective Control ◽  
Control Effect ◽  
Tomato Production ◽  
Combined Use ◽  
Promote Plant Growth ◽  
Tomato Bacterial Wilt

AbstractTomato bacterial wilt (BW) caused by Ralstonia solanacearum seriously restricts tomato production and no effective control measures are available. A microbial restoration substrate (MRS) had been proved to be effective control of tomato BW in a greenhouse cultivation. In this study, MRS was combined with an avirulent Ralstonia solanacearum (aRS) strain to control the disease under an open field condition. In the two consecutive year (2017 and 2018) trials, the combined use of aRS and MRS resulted in better disease control compared with either aRS or MRS alone. Moreover, the combined treatment was more effective than expected and suggesting a synergistic control effect. Compared with control (CK, non-aRS or MRS), the application of aRS and MRS treatments alone or in combination could all promote plant growth, increase root activity and yield (e.g. the yield for the treatment of aRS + MRS increased by 463.64% in 2017). Soil nutrients, including soil organic carbon, total nitrogen, total phosphorus and total potassium contents were also significantly increased by the application of aRS and MRS treatments alone or in combination (P < 0.05). The application of MRS or in combination with aRS changed the soil from acidic to neutral, which is one of the key factors for controlling BW. The soil enzymatic activities were notably influenced by the combined use of aRS and MRS, which increased urease (87.37% in 2017 and 60.89% in 2018), catalase (93.67% in 2017 and 279.37% in 2018) and alkaline phosphatase activities (193.77% in 2017 and 455.73% in 2018). These results suggest that the combination of MRS and aRS could effectively control tomato BW and thus represents a promising new tool to control this disease.

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