Evaluation of phytochemical constituents and in vitro antimicrobial activities of leaves extracts of Calotropis procera against certain human pathogens

Calotropis procera, a well-known and commonly used plant in Nigeria to treat many infectious diseases, has been documented to possess constituents with proven antimicrobial potentials. The type of solvent used in herbal extraction determines the number of phytoconstituents extracted, therefore it was investigated the effect of some solvents selected based on polarity on the phytoconstituents and antimicrobial efficacy of leaves of C. procera. Phytochemical screening of extracts from leaves of C. procera obtained from five solvents (acetone, hexane, ethylactate, mixture “acetone, hexane, ethyl acetate 50/30/20 v/v/v” and sterile distilled water) was carried out. Disc diffusion assay, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimal Fungicidal Concentration (MFC) of the extracts were compared with those of commercial drugs (chloramphenicol, gentamicin and griseofulvin) against Candida albicans, S. aureus ATCC 25923 and E. coli ATCC 25922. Tannins, steroids, alkaloids, saponins, glycosides, terpenoids, proteins, flavonoids, coumarins and anthraquinones were detected in higher numbers in ethyl acetate and sterile distilled water extracts, while the least number of constituents were detected in hexane extracts. A dose- dependent broad spectrum of bacteriostatic/fungistatic and bactericidal activities was produced by the extracts against the tested pathogens. Wider inhibition zone (19.80 mm) was produced at 100 mg/mL concentration by the mixture of solvents and ethylacetate extract, respectively, while the lowest inhibition zone (7.6 mm) was produced by sterile distilled water extract at 25 mg/mL. The present study provided plausible information to corroborate the medicinal value of the investigated plant, as claimed by herbal medicine users.

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In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

The Open Microbiology Journal ◽

10.2174/1874285801711010352 ◽

2017 ◽

Vol 11 (1) ◽

pp. 352-359 ◽

Cited By ~ 4

Author(s):

Gemechu Ameya ◽

Aseer Manilal ◽

Behailu Merdekios

Keyword(s):

Nicotiana Tabacum ◽

Medicinal Plants ◽

Ethyl Acetate ◽

Organic Solvents ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Modern Medicine ◽

Phytochemical Analysis ◽

Human Pathogens

Background: Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria. Methods: Nicotiana tabacum L. was collected from the Western Ethiopia and extracted in seven organic solvents. An in-vitro anti-bacterial activity of plant extracts was carried out by agar well diffusion assay against microbial type culture collection of human pathogens, clinical bacterial isolates, and biofilm forming bacteria. Gas Chromatographic and Mass Spectroscopic (GC-MS) analysis was used to determine the phytochemical constituents. Results: Antimicrobial activities of plant extract vary by extraction solvents; and ethyl acetate based extracts showed better antimicrobial activities. Of the experimental organisms, biofilm forming uropathogens were the most sensitive while clinical isolates were quite resistant. Analysis of the active ethyl acetate extract by GC-MS evinced a mixture of five volatile compounds; and Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) was the major compound detected. The overall results of the present study revealed that N. tabacum L extract has high antimicrobial activities against biofilm forming uropathogens. Conclusion: High antimicrobial activity was observed in ethyl acetate extract of N. tabacum against the biofilm forming bacteria whereas the clinically isolated bacteria were the most resistant group. The antibacterial property demonstrated could be due to Pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S) with a broad spectrum of activity.

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Introduction pages

Notulae Scientia Biologicae ◽

10.15835/nsb12210769 ◽

2020 ◽

Vol 12 (2) ◽

pp. I-IX

Author(s):

Radu E. SESTRAS

Keyword(s):

Shoot Multiplication ◽

Western Himalaya ◽

Antimicrobial Activities ◽

Calotropis Procera ◽

Syzygium Aromaticum ◽

Human Pathogens ◽

Temporary Immersion System ◽

Vitro Effect

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 2, Volume 12, 2020: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting researches, we invite readers to find news about: evaluation of phytochemical constituents and in vitro antimicrobial activities of leaves extracts of Calotropis procera against certain human pathogens; in vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia; impact of essential oils of clove Syzygium aromaticum in rats exposed to stress by nickel; young capitulum as important explant in in vitro mass propagation of gerbera (Gerbera jamesonii); a comparative study between temporary immersion system and semi-solid cultures on shoot multiplication and plantlets production of two Moroccan date palm (Phoenix dactylifera L.) varieties in vitro; effect of pretreatments on germination of seeds of the timber plant, Terminalia ivorensis and Mansonnia altissima; abundance, frequency and distribution pattern of tree species in recorded forest area of Western Himalaya; role of active transport of potassium to leaves in the mechanisms of tolerance to salinity in common bean (Phaseolus vulgaris L.).

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Chemical analysis and evaluation of antioxidant and antimicrobial activities of fruit fractions ofMauritia flexuosaL. f. (Arecaceae)

PeerJ ◽

10.7717/peerj.5991 ◽

2018 ◽

Vol 6 ◽

pp. e5991 ◽

Cited By ~ 7

Author(s):

Carla de Fatima Alves Nonato ◽

Débora Odília Duarte Leite ◽

Rafael Caldas Pereira ◽

Aline Augusti Boligon ◽

Jaime Ribeiro-Filho ◽

...

Keyword(s):

Ethyl Acetate ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Antifungal Drugs ◽

Resistant Bacteria ◽

Array Detector ◽

Chloroform Fraction ◽

Standard Samples ◽

Antioxidant And Antimicrobial Activities

BackgroundFruit consumption is currently considered beyond the nutritional aspects because of the important roles in disease prevention and benefits to health. The objective of this study was to characterize the chemical profile and evaluate the antioxidant and antimicrobial properties of different fractions obtained from fruit pulps ofMauritia flexuosa(MFFs).MethodsInitially, chloroform, ethyl acetate and ethanol fractions were obtained from the pulps. Quantifications of total phenols and flavonoids were performed using the methods of Folin-Ciocalteu and complexation with aluminum chloride, respectively. Standard samples were used to identify and quantify phenolic acids and flavonoids using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). The antioxidant capacity of the fractions was verified by sequestration of the free radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and iron chelating activity. The antimicrobial activity was determined using the microdilution method and the modulating activity was determined using sub-inhibitory concentrations of the fractions in association with antibiotics.ResultsThe chemical analyzes revealed the presence of catechin, caffeic acid, rutin, orientin, quercetin, apigenin, luteolin and kaempferol, where all are present in the ethyl acetate fraction. The fractions exhibited moderate antioxidant and antimicrobial activities against Gram-positive andCandidastrains in addition to modulating the activity of conventional antibiotics. The most expressive result was obtained from the association of the chloroform fraction with cefotaxime, which produced a synergistic effect, reducing the minimum inhibitory concentration (MIC) of the antibiotic from 1,024 to 256 μg/mL.DiscussionThe fractions presented a constitution rich in phenolic compounds, especially flavonoids. The data obtained demonstrated that the fractions presented moderate antioxidant activity by acting both as primary and secondary antioxidants. The fractions presented antimicrobial and antibiotic potentiating activities, being the first record of modulating effect of fractions of this species against the studied microbial strains, but failed in modulating the activity of antifungal drugs, indicating that this plant has the potential to be used in the development of therapeutic alternatives against resistant bacteria. The constitution phenolic the fractions may be responsible for their pharmacological properties in vitro.

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Isolation and antimicrobial effects of endophytic fungi from Edgeworthia chrysantha

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i3.23575 ◽

2015 ◽

Vol 10 (3) ◽

pp. 529 ◽

Cited By ~ 4

Author(s):

Huawei Zhang ◽

Chuanfeng Ruan ◽

Xuelian Bai

Keyword(s):

Ethyl Acetate ◽

Endophytic Fungi ◽

Fermentation Broth ◽

Fungal Endophytes ◽

Antimicrobial Effect ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Human Pathogens ◽

Antimicrobial Effects ◽

Staphyloccocus Aureus

Ten fungal strains isolated from Edgeworthia chrysantha, one of traditional medicinal plants in China, were evaluated their antimicrobial activities against three human pathogens, Escherichia coli, Staphyloccocus aureus and Candida albicans, and two phytopathogens, Rhizoctonia cerealis and Colletotrichum gloeosporioides. The results indicated that most ethyl acetate extracts of fermentation broth of these fungal endophytes had stronger antimicrobial activities than their fermentation broth. Among these endophytic strains, both fermentation broth and the ethyl acetate extract of strain D showed the strongest inhibitory effects on all pathogens. Strains 5-19 and BZ also exhibited potent antibacterial activities. However, other strains had weak or no antimicrobial effect. This was the first report on the isolation and antimicrobial effects of endophytic fungi from E. chrysantha.

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The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

Bacterial Empire ◽

10.36547/be.2019.2.1.19-25 ◽

2019 ◽

Vol 2 (1) ◽

pp. 19

Author(s):

Murni Halim

Keyword(s):

Leaf Extract ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Natural Drugs ◽

Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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Functional Effects of Pomegranate Peel Extracts on Milk: Antibacterial Measurements, Antioxidant Activities, and Photochemical Characterizations

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2021.2097 ◽

2021 ◽

Vol 15 (5) ◽

pp. 571-579

Author(s):

Rokayya Sami ◽

Ebtihal Khojah ◽

Mona Alharbi ◽

Amina A. M. Al-Mushhin ◽

Mohammed Saeed Alkaltham ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Water Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Pomegranate Peel ◽

Bacteria Growth ◽

Wonderful Variety ◽

Ic50 Values

Consumers are interested in antimicrobial by-products from natural sources. The present study aims to estimate the antibacterial effects of different concentrations and solvent extracts such as methanol, ethanol, and water extract of pomegranate peels with the milk quality application in-vitro. Listeria monocytogens, E. coli, Salmonella, and Staphylococcus aureus were used as bacteria growth indicators in two pomegranate varieties (i.e., Wonderful and Francis). Besides, evaluating somephysical characterization, antibacterial and antioxidant activities. Results showed that the highest inhibition zone of 18.88 mm was observed with 90% ethanol extract of Wonderful variety against Listeria monocytogens, at 0, 12.5, 25, and 50 mg/mL concentrations, while the lowest inhibition zone of 11.29 mm was observed with 90% methanol extract of Francis variety, at 12.5 mg/mL concentration. The milk treated with WPE of Wonderful variety maintained the bacterial growth from 1.19 ±0.29 x 109 to 1.43 x 1011 CFU/mL at 4 °C. Punicalagin was the most abundant polyphenolic compound (165.05–190.43 µg/mL) followed by gallic acid (153.08–177.65 µg/mL), and p-Hydroxybenzoic acid (91.29–92.5 mg/mL). Acetaldehyde (23.09–27.15 PPM), followed by acetoin (9.65–15.33 PPM) were the most predominant volatile compounds. The WPE treated milk maintained the sensory evaluations such as taste, color, texture, and overall acceptance longer time longer time (i.e., 144 h) than FPE treated milk (i.e., 168 h) at 4°C. DPPH and ABTS radical scavenging of capacity (IC50 values) of FPE treated milk were found to be lower than WPE treated milk (i.e., 159.65 µg/mL and 131.87 µg/mL), respectively. While, FPE treated milk reported a higher viscosity content than WPE treated milk (13.11 cP). In conclusion, pomegranate peel extracts especially Wonderful variety may be used to prolong the shelf-life of milk in dairy products manufacturers.

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Antimicrobial Effects of Selected, Cultivated Red Seaweeds and Their Components in Combination with Tetracycline, against Poultry Pathogen Salmonella Enteritidis

Journal of Marine Science and Engineering ◽

10.3390/jmse8070511 ◽

2020 ◽

Vol 8 (7) ◽

pp. 511

Author(s):

Garima Kulshreshtha ◽

Alan Critchley ◽

Bruce Rathgeber ◽

Glenn Stratton ◽

Arjun H. Banskota ◽

...

Keyword(s):

Salmonella Enteritidis ◽

Combined Treatment ◽

Water Extract ◽

Antimicrobial Activities ◽

Human Consumption ◽

Drug Resistant ◽

Red Seaweeds ◽

Industry Standard ◽

Prophylactic Use

Poultry and its products are an economical source of high-quality protein for human consumption. In animal agriculture, antibiotics are used as therapeutic agents to treat disease in livestock, or as prophylactics to prevent disease and in so doing enhance production. However, the extensive use of antibiotics in livestock husbandry has come at the cost of increasingly drug-resistant bacterial pathogens. This highlights an urgent need to find effective alternatives to be used to treat infections, particularly in poultry and especially caused by drug-resistant Salmonella strains. In this study, we describe the combined effect of extracts of the red seaweeds Chondrus crispus (CC) and Sarcodiotheca gaudichaudii (SG) and compounds isolated from these in combinations with industry standard antibiotics (i.e., tetracycline and streptomycin) against Salmonella Enteritidis. Streptomycin exhibited the higher antimicrobial activity against S. Enteritidis, as compared to tetracycline with a MIC25 and MIC50 of 1.00 and 1.63 μg/mL, respectively. The addition of a water extract of CC at a concentration of 200 µg/mL in addition to tetracycline significantly enhanced the antibacterial activity (log CFU/mL 4.7 and 4.5 at MIC25 and MIC50, respectively). SG water extract, at 400 and 800 µg/mL (p = 0.05, n = 9), also in combination with tetracycline, showed complete inhibition of bacterial growth. Combinations of floridoside (a purified red seaweed component) and tetracycline (MIC25 and MIC50) in vitro revealed that only the lower concentration (i.e., 15 μg/mL) of floridoside potentiated the activity of tetracycline. Sub-lethal concentrations of tetracycline (MIC50 and MIC25), in combination with floridoside, exhibited antimicrobial activities that were comparable to full-strength tetracycline (23 μg/mL). Furthermore, the relative transcript levels of efflux-related genes of S. Enteritidis, namely marA, arcB and ramA, were significantly repressed by the combined treatment of floridoside and tetracycline, as compared to control MIC treatments (MIC25 and MIC50). Taken together, these findings demonstrated that the red seaweeds CC and SG and their selected, purified components can be used to increase the lifetime of existing, patented antibiotics and can also help to reduce costly (economic and environmental) therapeutic and prophylactic use of antibiotics in poultry. To our knowledge, this is the first report of antibiotic potentiation of existing industry standard antibiotics using red seaweeds and their selected extracts against S. Enteritidis.

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Composition, In Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained from Black Cumin Seeds (Nigella sativaL.)

BioMed Research International ◽

10.1155/2014/918209 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 24

Author(s):

Sunita Singh ◽

S. S. Das ◽

G. Singh ◽

Carola Schuff ◽

Marina P. de Lampasona ◽

...

Keyword(s):

Essential Oil ◽

Ethyl Acetate ◽

Linseed Oil ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Black Cumin

Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%),α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as11.47±0.05,10.88±0.9,9.68±0.06, and8.33±0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition againstFusarium moniliformein inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria.

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Analysis of Antimicrobial Activities of Photoactivated Cow Urine against Common Human Pathogens

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20jun799 ◽

2020 ◽

Vol 5 (6) ◽

pp. 1557-1560

Author(s):

Monica A. Godad ◽

Siddhi S. Suryavanshi ◽

Somesh S. Shintre ◽

Kiran K. Abitkar

Keyword(s):

Inhibition Zone ◽

Antimicrobial Activities ◽

Human Pathogen ◽

Human Pathogens ◽

Bacterial Strains ◽

Zone Of Inhibition ◽

Average Width ◽

Gram Negative ◽

Cow Urine ◽

Escherichi Coli

A concurrent and promising effects has been achieved by using cow urine as a efficient antibacterial activity against gram-negative and gram-positive human pathogen. The present study aims to evaluate anti-microbial activity photo activated cow urine against pathogenic bacterial strains. It is estimated by agar well diffusion method.It is evident from the data presented that the zone of inhibition of photo activated Gir cow urine and Khillari cow urine against bacteria Escherichi coli and staphulococcus aureus is significant.The present investigation clearly demonstrate that the average width of inhibition zone at 50 micrilitre dose was 1.6cm.Therefore this pathogen can be well managed by using cow urine.

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Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro

Jurnal MIPA ◽

10.35799/jm.2.2.2013.3121 ◽

2013 ◽

Vol 2 (2) ◽

pp. 128 ◽

Cited By ~ 2

Author(s):

Mercy Ngajow ◽

Jemmy Abidjulu ◽

Vanda S. Kamu

Keyword(s):

Staphylococcus Aureus ◽

Secondary Metabolites ◽

Antibacterial Effect ◽

Inhibition Zone ◽

Stem Bark ◽

Positive Control ◽

Negative Control ◽

Bark Extract ◽

Distilled Water

Telah dilakukan penelitian secara kualitatif terhadap pengaruh antibakteri dari ekstrak kulit batang matoa (Pometia pinnata. Spp.) terhadap bakteri Gram positif Staphylococcus aureus . Sebelum dilakukan uji antibakteri, sampel yang telah diekstrak secara maserasi diuji fitokimia terlebih dahulu untuk menentukan kandungan metabolit sekunder yang telah diketahui berperan sebagai agen antibakteri. Setelah dilakukan uji fitokimia, ekstrak diuji aktivitas antibakterinya terhadap bakteri Staphylococcus aureus dengan menggunakan teknik difusi agar dengan cara sumuran. Ekstrak dilarutkan pada aquades steril dengan perbandingan 2 g ekstrak pada 2 mL air. Untuk kontrol positif, digunakan ciprofloxacin dan aquades steril sebagai kontrol negatif. Hasil penelitian ini menunjukkan bahwa ekstrak kulit batang matoa memiliki aktivitas antibakteri setelah diinkubasi selama 24 jam pada 37 OC. Dari 3 kali pengulangan dengan masing – masing 3 sumuran, didapat zona hambat masing – masing 16.84 mm, 12.5 mm dan 14.5 mm dengan kontrol positif 29.67 mm serta kontrol negatif 0 mm. Hasil yang diperoleh didukung oleh keberadaan metabolit sekunder hasil uji fitokimia yaitu tanin, flavonoid, terpenoid dan saponin.A qualitative study has done of the antibacterial effect of matoa (Pometia pinnata) stem bark extract against Gram-positive bacteria Staphylococcus aureus . Before the antibacterial test , samples were extracted by maceration and then phytochemical tested to measuring the content of secondary metabolites that have been known to act as an antibacterial agent . After being tested of phytochemical , extracts were tested the antibacterial effect against Staphylococcus aureus using agar diffusion technique. Extract was dissolved in sterile distilled water with a ratio of 2 g of extract in 2 mL of water . For the positive control , use of ciprofloxacin and sterile distilled water as a negative control . Results of this study indicate that matoa bark extract has antibacterial effect after incubation for 24 h at 37OC . Of 3 times with each repetition - each 3 wells, the inhibition zone obtained - each 16.84 mm , 12.5 mm and 14.5 mm with 29.67 mm of positive control and a negative control by 0 mm . The results are supported by the presence of secondary metabolites by phytochemical test such as tannins , flavonoids , terpenoids and saponins.

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