scholarly journals EFFECT OF FEED ADDITIVE ON MINERAL HOMEOSTASIS OF COWS WITH KETOSIS

Author(s):  
O. Shkilnyk ◽  
I. Petrukh ◽  
D. Ostapiv ◽  
M. Kozak ◽  
V. Vlizlo ◽  
...  

The article describes the study of mineral metabolism in patients with cow ketosis and during feeding of our developed feed additive, which includes crushed hop cones, vitamin E and protected from cleavage in the rumen amino acids ˗ methionine, choline and carnitine. In the blood of cows with ketosis, before and after the use of feed additives studied the content of total calcium and inorganic phosphorus, alkaline phosphatase activity, determined the ratio of alkaline phosphatase and total calcium, the concentration of thyroid hormones (parathyroid hormone) and thyroid, thyroid also the amount of ketone bodies in the urine and β-hydroxybutyrate in the blood. It was found that twenty days of feeding cows with ketosis, feed additives leads to a decrease in ketonuria and ketonemia, as well as the normalization of mineral metabolism. At the end of the experiment, the content of total calcium and inorganic phosphorus in the blood of cows increased, the activity of alkaline phosphatase and the ratio of ALP / Ca decreased. The normalization of mineral metabolism in cows was also evidenced by its balanced hormonal regulation. The concentration of parathyroid hormone decreased in the blood plasma and calcitonin increased. According to the results of research, it is established that our proposed feed additive contributes to the normalization of mineral metabolism, and can also be used for prevention and in the complex treatment of cows with ketosis.

Author(s):  
A. V. Trebukhov ◽  
A. A. Elenshleger

Under present-day conditions of market economy, the requirements to the quality and quantity of livestock products increase significantly, and this leads to significant intensification of production and, as a consequence, to the development of metabolic disorders including ketosis in highly productive cows. The research goal was to study protein and mineral metabolism in ketotic cows before and after calving. The studies were conducted on the Training Farm «Prigorodnoye» in the City of Barnaul in autumn and winter in comparable Black-Pied cows. Two groups of cows were formed: the trial group consisted of ketotic cows; the control group consisted of apparently healthy cows. These cows underwent clinical and biochemical examination. The biochemical study of blood determined the following: total protein, albumins, alpha-globulins, beta-globulins, gamma-globulins, total calcium, inorganic phosphorus and ketone bodies. The biochemical blood tests were run 4 times: 2 months and 1 month before calving, and in 10 days and in 1 month after calving. It was found that the change of protein metabolism indices in ketotic cows showed a profound disorder of the protein synthesis function of liver both before and after calving. The level of total calcium in the blood of ketotic cows was characterized by lower values as compared to that in healthy comparable cows. At the same time, the dynamics of inorganic phosphorus concentration change did not have significant differences between ketotic and healthy cows. Total calcium content in ketotic cows increased one month before calving while in apparently healthy cows this index increased after calving only. The concentration of ketone bodies in the blood of ketotic cows was significantly higher than that of apparently healthy cows throughout the study.


Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.


1993 ◽  
Vol 74 (2) ◽  
pp. 114-116
Author(s):  
N. M. Gruber

The levels of inorganic phosphorus, calcium, free, connected and common oxyproline, alkaline phosphatase activity in blood are investigated in the experiment of standard craniocerebral trauma in connection wth the fracture of crus bones. The data obtained suggest the breaking of the coordination of hormonal regulation, manifesting itself simultaneously by the effect of parathormone and calcitonine in both phases of osteal regeneration. The disregulation of mineral metabolism, catabolism and anabolism processes of organic matrix causes the delay of reparative processes, intypicalness and development of fibrous tissue.


2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.


Author(s):  
N.F. Sadykov ◽  
◽  

Metabolic diseases are more common in highly productive cows. The use of feed additive No. 1 led to positive changes in the health and milk productivity of cows in SPH «Nm.Vakhitova». In comparison with the control group, an increase in the mass fraction of fat and protein, a decrease in somatic cells and ketone bodies were observed in all experimental groups over the entire period of the experiment.


Author(s):  
К.А. ГЕРЦЕВА ◽  
Д.В. ДУБОВ ◽  
А.В. КАДЫРОВ ◽  
Е.В. КИСЕЛЕВА ◽  
А.В. СИТЧИХИНА

В научной работе изучена эффективность профилактики внутренних незаразных болезней в послеродовом периоде у коров путем введения в рацион глубоко сухостойных коров кормовой добавки «Кауфит драй плюс». Эффективность кормовой добавки оценивали, анализируя частоту встречаемости незаразной патологии, данные физикального осмотра, морфологические и биохимические изменения в крови, рН-мочи и рубцовой жидкости. Исследования проводились на двух группах аналогов глубоко сухостойных коров (контрольная и опытная). Было установлено, что на 21-й день после отела в опытной группе по сравнению с контролем достоверно уменьшилась частота встречаемости: задержания последа на 11,7%, послеродового эндометрита на 10,0%, родильного пареза на 8,3%, клинического мастита на 8,4%. Однако случаи заболевания смещения сычуга за исследуемый период в опытной группе увеличились на 1,6%, кетоза – на 3,3%, гипотонии рубца – на 3,3%, асептических болезней копыт – на 4,9%. В опытной группе отмечено достоверное повышение кислотности рубцовой жидкости (с 6,82±0,38 до 5,41±0,24, р<0,01) и мочи (с 8,23±0,18 до 6,53±0,12, p<0,01); произошло достоверное снижение уровня глюкозы на 39,4% (p<0,01), увеличение содержания кетоновых тел в 2,8 раза, повышение уровня кальция и фосфора на 22,4 и 18,2% (p<0,05) соответственно. Обнаруженные изменения в биохимическом профиле крови исследуемых коров свидетельствуют о развитии энергетического дефицита в организме коров опытной группы и развитии гипокальциемии в организме коров контрольной группы. Применение кормовой добавки «Кауфит Драй плюс» достоверно снижает риск развития гипокальциемии, родильного пареза, послеродового эндометрита, задержания последа и клинического мастита, но увеличивает риск развития таких болезней в послеродовом периоде как гипотония рубца, кетоз, смещение сычуга, асептических болезней копыт. The effectiveness of prevention of internal non-contagious diseases in the postpartum period in cows due to the introduction of a feed additive "Kaufit dry plus" into the diet of deeply dry cows was studied in the scientific work. The effectiveness of the feed additive was evaluated by analyzing the incidence of non-infectious pathology, physical examination data, morphological and biochemical changes in blood, pH-urine and scar fluid. The studies were carried out on two groups of analogues of deeply dry cows (control and experimental). It was determined that on 21 days after calving in the experimental group, compared with the control, the incidence of retention of the afterbirth significantly decreased by 11.7%, postpartum endometritis by 10.0%, puerperal paresis by 8.3%, clinical mastitis by 8.4%. However, cases of abomasal displacement during the study period in the experimental group increased by 1.6%, ketosis - by 3.3%, scar hypotension - by 3.3%, aseptic hoof diseases-by 4.9%. In the experimental group, there was a significant increase in the acidity of scar fluid (from 6.82±0.38 to 5.41±0.24, p<0.01) and urine (from 8.23±0.18 to 6.53±0.12, p<0.01); there was a significant decrease in glucose by 39.4 % (p<0.01), an increase in the content of ketone bodies by 2.8 times, an increase in calcium and phosphorus by 22.4% and 18.2% (p<0.05), respectively. The detected changes in the biochemical profile of the blood of the studied cows indicate the development of energy deficiency in the body of cows of the experimental group and the development of hypocalcemia in the body of cows of the control group. The use of the feed additive "Kaufit dry plus" significantly reduces the risk of hypocalcemia, puerperal paresis, postpartum endometritis, retention of the afterbirth and clinical mastitis, but increases the risk of such diseases in the postpartum period as hypotension of the scar, ketosis, displacement of the rennet, aseptic diseases of the hooves.


Author(s):  
Jialin Li ◽  
Danni He ◽  
Wenjing Zhao ◽  
Xi’ai Wu ◽  
Minjing Luo ◽  
...  

AbstractBackgroundWe aimed to examine the association of three mineral metabolism markers, including serum calcium, inorganic phosphorus, and intact parathyroid hormone with the risk of chronic kidney disease (CKD) at all stages.MethodsThis retrospective cohort study involved 3563 participants, including 3274 CKD patients and 289 healthy controls. CKD is diagnosed according to clinical guidelines from the 2012 KDIGO. Effect sizes are expressed odds ratio (OR) and 95 confidence interval (CI).ResultsAfter propensity score matching, per 0.5 mg/dL increment of inorganic phosphorus was significantly associated with 1.33-, 1.61-, and 2.85-fold increased risk of CKD at stages 1–2, 4, and 5, respectively. Regarding per 8 pg/mL increment of intact parathyroid hormone, significance was only noted for stage 5. In subsidiary analyses, the risk prediction of mineral metabolism markers under study was more evident in males and hypertensive subjects. A nomogram prediction model was constructed based on age, sex, and three mineral metabolism markers for CKD, with decent accuracy.ConclusionsOur findings indicate that serum calcium was associated with all-stage CKD risk, whereas the association for inorganic phosphorus and intact parathyroid hormone was significant at advanced stages.


1990 ◽  
Vol 78 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Sergio De Marchi ◽  
Emanuela Cecchin

1. To investigate mechanisms of extrarenal buffering in uraemic acidosis, we studied the effects of the carbonic anhydrase inhibitor, acetazolamide, in normal subjects and in patients with end-stage kidney disease on maintenance haemodialysis with virtually no urine output. 2. Acetazolamide (500 mg) was administered daily for 7 days, after pretreatment for 1 month with 1,25-dihydroxyvitamin D (n = 12) or placebo (n = 12); only placebo was administered to a third group (n = 12) of haemodialysis patients. In addition, acetazolamide was administered to normal control subjects (n = 12). 3. Treatment with acetazolamide resulted in a more marked metabolic acidosis in haemodialysis patients than in normal control subjects and the effect in haemodialysis patients was attenuated by prior treatment with 1,25-dihydroxyvitamin D. 4. The administration of acetazolamide to haemodialysis patients led to an increase in serum inorganic phosphorus, bone isoenzyme of alkaline phosphatase and parathyroid hormone, and a reduction in serum calcium, whereas acetazolamide had no effect on these variables in normal subjects. In contrast, in the haemodialysis patients previously treated with 1,25-dihydroxyvitamin D, acetazolamide increased serum inorganic phosphorus, bone isoenzyme of alkaline phosphatase, parathyroid hormone and serum calcium. 5. We hypothesize that the metabolic acidosis induced by acetazolamide in haemodialysis patients may result from interference with the mechanisms of extrarenal buffering. 6. As parathyroid hormone, 1,25-dihydroxyvitamin D and carbonic anhydrase are thought to be involved in bone buffering, we suggest that the marked acidosis seen in haemodialysis patients treated with acetazolamide may be due to impaired parathyroid hormone-mediated bone buffering.


Author(s):  
N. I. Bodnaruk ◽  
T. Y. Lysak

Musculoskeletal disorders (MSD) occupy a leading place among the diseases affecting the dental health of children. The composition and properties of the oral fl uid are an appropriate refl ection of the various processes in the body and can help to study the pathogenesis of tooth decay associated with MSD.The aim of the study – to determine mineral ingredients composition of saliva in children with MSD and to identify its relationship with dental caries.Materials and Methods. 90 5-year old children were examined and divided into three groups of 30 persons in each: fi rst – children with dental caries and MSD; the second – children with dental caries, MSD and undifferentiated connective tissue disease (UCTD); the third – children with dental caries without somatic pathology (comparison group). The deft values and levels of caries prevalence were evaluated. Mineral components of the saliva were determined by photometric method.Results and Discussion. The deft value was (6.17±0.52) in children with MSD, (8.00±0.50) in children with MSD and UCTD and (4.37±0.47) in children of the comparison group. In children with MSD total calcium level in the saliva was 33.80 % lower compared to that in comparison group and 25.53 % higher than in children with MSD and UCTD. The concentration of inorganic phosphorus in the saliva of children with MSD was 29.73 % lower compared to that in comparison group and 15.71 % higher than in children with MSD and UCTD. The same pattern has shown the magnesium level. Alkaline phosphatase activity in the saliva of both groups of children with MSD was lower than in comparison group.Conclusions. In the saliva of children with dental caries and MSD the defi ciencies of total calcium, inorganic phosphorus and magnesium, as well as reduced activity of alkaline phosphatase were revealed.


2019 ◽  
Vol 25 ◽  
pp. 107602961989662
Author(s):  
Aleksander Druck ◽  
Dimpi Patel ◽  
Vinod Bansal ◽  
Debra Hoppensteadt ◽  
Jawed Fareed

Chronic kidney disease stage 5 (CKD5) marks the fifth stage of renal failure, frequently causing dysregulation of bone and mineral metabolism. Challenges exist in evaluating and managing chronic kidney disease–mineral bone disorder (CKD-MBD) with the standard panel of biomarkers. Our objective was to profile osteopontin (OPN) in patients with CKD5 on maintenance hemodialysis (CKD5-HD) and elucidate its relationship to phosphorus (P), calcium (Ca2+), alkaline phosphatase (AP), and intact parathyroid hormone (iPTH) to improve understanding of the present model of CKD-MBD. Elevation of plasma OPN was seen in the CKD5-HD cohort (n = 92; median: 240.25 ng/mL, interquartile range [IQR]: 169.85 ng/mL) compared to a normal group (n = 49; median: 63.30 ng/mL, IQR: 19.20 ng/mL; p < .0001). Spearman correlation tests revealed significant positive correlations of OPN with iPTH ( p < .0001; r = 0.561, 95% confidence interval = 0.397-0.690) and OPN with AP ( p < .0001; r = 0.444, 95% confidence interval = 0.245-0.590) in CKD5-HD patients. Ultimately, OPN may play an integral role in the MBD axis, suggesting that it may be important to actively monitor OPN when managing CKD5-HD.


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