scholarly journals Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

2019 ◽  
Vol 49 (1) ◽  
pp. 41-47
Author(s):  
Ana Paula Simões da CUNHA ◽  
Luana BALDISSERA ◽  
Débora Linsbinski PEREIRA ◽  
Lucineia Reuse ALBIERO ◽  
Lindsey CASTOLDI ◽  
...  
Keyword(s):  
Day Treatment ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Stem Bark ◽  
Protein Carbonylation ◽  
Antioxidant Compounds ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Ehrlich Tumor ◽  
Biochemical Analyses

ABSTRACT Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST)], non-enzymatic antioxidants [reduced glutathione (GSH) and ascorbic acid (ASA)], substances reactive to thiobarbituric acid (TBARS) and protein carbonylation (carbonyl) in different tissues were significantly affected. The extract administered at 200 mg kg-1 presented higher antioxidant capacity in the liver, increased CAT, GST, GSH and decreased TBARS, as well as increased CAT activity and protein carbonylation in brain tissue. The results showed that the copaiba extract was able to reverse the oxidative stress caused by solid Ehrlich tumor, probably due to the presence of antioxidant compounds, and had potential antineoplasic effect after a 30-day treatment.

scholarly journals Antioxidant potential of Carica papaya Linn (Caricaceae) leaf extract in mice with cyclophosphamide induced oxidative stress

2020 ◽  
Vol 30 (1) ◽  
pp. e34702
Author(s):  
Tatiane Cordeiro Luiz ◽  
Ana Paula Simões Da Cunha ◽  
Danilo Henrique Aguiar ◽  
Marina Mariko Sugui ◽  
Rogério de Campos Bicudo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carica Papaya ◽  
Hematological Parameters ◽  
Thiobarbituric Acid ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Biochemical Tests ◽  
Red Cell Count ◽  
Phytochemical Profile

AIMS: This study aimed to investigate the effects of crude extract of Carica papaya leaves on oxidative stress in mice induced by cyclophosphamide, as well as phytochemical profile characterization of this extract.METHODS: The male Swiss mice received 15 days of treatment with the extract (500 mg kg-1, via gavage) and intraperitoneal injection of cyclophosphamide (75 mg kg-1) or saline (0.9%) on the 15th day. After 24 h the last treatment, the animals were anesthetized for blood withdrawal, sacrificed and removal of the organs for analyses (liver, kidney and heart). In the biochemical tests were determined: hematological parameters in blood, aminotransferases, alkaline phosphatase, glucose and total cholesterol dosages in plasma, enzymatic and non-enzymatic antioxidants and lipid damage marker were evaluated in different tissues, besides genotoxic and histopathological analyzes.RESULTS: In the extract of Carica papaya leaves, the flavonoids quercetin-3β-D-glucoside and rutin were identified, besides present positive results for alkaloids, saponins and tannins. This extract increased the activity of glutathione-S-transferase and catalase enzymes in the liver and reduced the levels of reduced glutathione in the kidneys and hematocrit levels, red cell count, and hemoglobin. It promoted the decrease of the reactive species of thiobarbituric acid (TBARS) in the kidneys and the activity of enzyme aspartate aminotransferase in the plasma and was antimutagenic in the micronucleus test.CONCLUSIONS: The study showed that extract of Carica papaya was beneficial against oxidative events and prevented DNA damage. The extract also showed hepatotoxicity, therefore prolonged infusion of papaya leaves is not advisable.

scholarly journals Antioxidant activity of bee products added to water in tebuconazole-exposed fish

2012 ◽  
Vol 10 (1) ◽  
pp. 215-220 ◽  
Author(s):  
Daiane Ferreira ◽  
Taís Cristina Unfer ◽  
Hélio Carlos Rocha ◽  
Luiz Carlos Kreutz ◽  
Gessi Koakoski ◽  
...  
Keyword(s):  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
South American ◽  
Enzymatic Antioxidants ◽  
Thiobarbituric Acid Reactive Substances ◽  
Glutathione S Transferase ◽  
Exposed Fish ◽  
Bee Pollen ◽  
Protein Thiols ◽  
Oxidant Effect

An experiment was conducted to evaluate the potential of honey, propolis, and bee pollen for the reversal of lipid peroxidation induced by tebuconazole (TEB) in South American catfish (Rhamdia quelen), in which the concentration of thiobarbituric acid reactive substances (TBARS), the activity of the antioxidant enzyme glutathione-S-transferase (GST) and the concentrations of non-enzymatic antioxidants, reduced glutathione (GSH), ascorbic acid, and non-protein thiols were assessed. Honey (0.125 g L-1) and bee pollen (0.05 g L-1) added to the water reverse the production of TBARS induced by TEB, while propolis demonstrated a pro-oxidant effect, inducing an increase in TBARS production. The data presented herein suggest that the addition of water to honey and bee pollen potentially protects against the oxidative stress caused by agrichemicals.

Antioxidant Activity of 2-Bromo-3-hydroxy-2-nitroproylcinnamte against Alcohol Induced Oxidative Damage

2020 ◽  
Vol 5 (2) ◽  
pp. 156-160
Author(s):  
Rajesh Kumar Malik ◽  
Dharmendra Kumar Singh ◽  
Anuradha ◽  
Surendra Kumar
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Reduced Glutathione ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Alcohol Concentration ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Β Carotene

The present study demonstrated the antioxidant activity of 2-bromo-3-hydroxy-2-nitroproylcinnamte versus alcohol induced oxidative damage in albino wistar rats. In this study, 30 % alcohol exposed rats were found to be more prone to peroxidative risk as they are calculated by species of thiobarbituric acid. It was observed that after the rats induction with 30% alcohol, concentration of lipid peroxidation has been obtained expressively (p ≤ 0.001) high in a liver and serum, beside with concomitant substantial (p ≤ 0.001) reduced in enzymatic and non-enzymatic antioxidants levels like catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), vitamin E (tocopherol), reduced glutathione (GSH), glutathione-s-transferase (GST), vitamin C (ascorbic acid), β-carotene as well as ceruloplasmin in serum along with liver, only 30% alcohol was treated. If rats obtained 2- bromo-3-hydroxy-2-nitroproylcinnamte at the dose level of 20 mg/kg bw/day, PO, for 30 days, the peroxidative damage has been marginal in serum along with liver, alongside efficiently encouraging the potential of antioxidant in the rats treated by alcohol. This study revealed that in liver the raised peroxidative risk is probably allied with alcohol induction pathology that can be decreased by increasing the antioxidant potential by free radical scavenging activity, therefore promising as artificial antioxidants for 2-bromo-3-hydroxy-2-nitroproylcinnamte.

scholarly journals EVALUATION OF HEPATOPROTECTIVE AND ANTIOXIDANT EFFECT OF COMBRETUM ALBIDUM G. DON AGAINST CCl4 INDUCED HEPATOTOXICITY IN RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 218
Author(s):  
D. Rajalingam ◽  
R. Varadharajan ◽  
S. Palani
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Experimental Models ◽  
Thiobarbituric Acid Reactive Substances ◽  
Glutathione S Transferase ◽  
Standard Drug ◽  
Whole Plant ◽  
Histopathological Studies ◽  
Dose Levels

<p><strong>Objective: </strong>The present investigations were undertaken to evaluate the hepatoprotective and antioxidant activity of the ethanolic extract of the whole plant of <em>Combretum albidum G </em>Don against CCl<sub>4</sub>-induced hepatotoxicity in rats.</p><p><strong>Methods</strong><strong>:</strong><strong> </strong>Hepatoprotective effect of ethanolic extract of <em>Combretum albidum</em> (EECA) was determined by using carbon tetrachloride (CCl<sub>4</sub>) intoxication of rats as experimental models. The extent of liver damage and effect of the plant extract was assessed by various biochemical parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total billrubin (TB) and total protein (TP) in blood serum and concentration of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-s-transferase (GST) in liver were determined. Histopathological changes in the liver of different groups were also studied.</p><p><strong>Results: </strong>The administration of EECA at dose levels of 250 and 500 mg/kg/b.w., orally had decreased the rise of ALT, AST, ALP, TB and TBRAS levels and the effects were comparable to standard drug (Silymarin 25 mg/kg/b. w,) the GSH, SOD, CAT, GPx, GST and TP levels were significantly increased in the animals received EECA. The histopathological studies show decreased necrosis and hepatocellular degeneration when compared to the CCl  intoxicated liver.</p><p><strong>Conclusion: </strong>This study demonstrates that the hepatoprotective and the antioxidant activity of the whole plant of <em>Combretum albidum</em> therefore scientifically supports the use of this plant in traditional medicine for treatment of liver disorders.</p>

Arsenic induced oxidative stress in plants

Biologia ◽  
2012 ◽  
Vol 67 (3) ◽  
Author(s):  
Iti Sharma
Keyword(s):  
Oxidative Stress ◽  
Photosynthetic Apparatus ◽  
Redox Status ◽  
Antioxidant Compounds ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Sh Groups ◽  
Structural Disorders ◽  
Toxic Metalloid ◽  
Major Section

AbstractArsenic is a highly toxic metalloid for all forms of life including plants. Arsenic enters in the plants through phosphate transporters as a phosphate analogue or through aquaglycoporins. Uptake of arsenic in plant tissues adversely affects the plant metabolism and leads to various physiological and structural disorders. Photosynthetic apparatus, cell division machinery, energy production, and redox status are the major section of plant system that are badly affected by As (V). Similarly As (III) can react with thiol (-SH) groups of enzymes and inhibits various metabolic processes. Arsenic is also known to induce oxidative stress directly by generating reactive oxygen species (ROS) during conversion of its valence forms or indirectly by inactivating antioxidant molecules through binding with their -SH groups. As-mediated oxidative stress causes cellular, molecular and physiological disturbances in various plant species. Activation of enzymatic antioxidants namely, superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR), Glutathione s-transferase, glutathione peroxidase (GPX) as well as non antioxidant compounds such as, ascorbate, glutathione, carotenoids are reported to neutralize arsenic mediated oxidative stress. Understanding of biochemistry of arsenic toxicity would be beneficial for the development of arsenic tolerant crops and other economically important plants.

scholarly journals First phytochemical study and biological activity of the leaves ethanolic extract from Cissus spinosa Cambess

2020 ◽  
Vol 30 (1) ◽  
pp. 34860
Author(s):  
Adilson Paulo Sinhorin ◽  
Bruna Serpa de Almeida Godoy ◽  
Valéria Dornelles Gindri Sinhorin ◽  
Lindsey Castoldi ◽  
Marina Mariko Sugui ◽  
...  
Keyword(s):  
Biological Effects ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Biological Action ◽  
Antioxidant Compounds ◽  
Enzymatic Antioxidants ◽  
Biochemical Tests ◽  
Phytochemical Study ◽  
Polychromatic Erythrocytes ◽  
Phytochemical Profile

AIMS: The objective of this study was to identify the phytochemical profile and to evaluate the biological effects of the crude ethanolic extract (EE) and the ethanolic fraction (EF) of leaves of the species Cissus spinosa Cambess, after oxidative stress induced by cyclophosphamide (CP) in mice.METHODS: Phytochemical profile was performed detecting functional groups and, analysis of total flavonoids and phenols concentration, as well as the antiradical activity in EE and EF. The phytochemical characterization was done for the identification of flavonoids present in the leaves of the plant. In the biochemical tests, hematological parameters, glucose and total cholesterol dosages in plasma, enzymatic and non-enzymatic antioxidants and lipid damage marker were evaluated in different tissues (liver, kidney and heart), besides genotoxic and immunological analyzes. The animals received 15 days of treatment, via gavage, with EE (50 mg kg-1) or EF (50 mg kg-1) and on the 15th day, an intraperitoneal injection of CP (100 mg kg-1) or saline (0.9%). After 24 h the last treatment, the animals were anesthetized for blood withdrawal, sacrificed and removal of the organs.RESULTS: In the phytochemical analyzes, the presence of alkaloids, flavonoids and phenols was identified, the latter presented a higher concentration for EF. Eight flavonoids were identified - Rutin, Quercetin-3-β-D-glucoside, Quercitrin, Taxifolin, Quercetin, Canferol, Luteolin and Apigenin. In the biochemical analyzes, in general, EE showed a better antioxidant action against oxidative damages, hypoglycemic and antitilipemic action when comparing with EF, probably due to the synergism caused by flavonoids. It was observed the reduction and an increase of micronucleated polychromatic erythrocytes, due to the action of antioxidant compounds and alkaloids present in the plant, also considering the question of the seasonal period that directly interferes in the production of these compounds. In the immunological analysis, the extracts did not stimulate the spontaneous production of oxygen peroxide (H2O2) and nitric oxide (NO•). CONCLUSIONS: Other studies, such as the variation of the chemical composition of the plant by local seasonality, hypoglycemic and antilipemic action, should be carried out to better delineate the biological action present in this plant.

scholarly journals Constituents of Chamaecrista diphylla (L.) Greene Leaves with Potent Antioxidant Capacity: A Feature-Based Molecular Network Dereplication Approach

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 681
Author(s):  
Paulo Gomes ◽  
Luis Quirós-Guerrero ◽  
Abraão Muribeca ◽  
José Reis ◽  
Sônia Pamplona ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Oxygen Radical ◽  
Molecular Network ◽  
Antioxidant Compounds ◽  
Herbaceous Plant ◽  
Network Approach ◽  
Feature Based ◽  
Promising Source ◽  
First Time

Chamaecrista diphylla (L.) Greene (Fabaceae/Caesalpiniaceae) is a herbaceous plant that is widely distributed throughout the Americas. Plants from this genus have been used in traditional medicine as a laxative, to heal wounds, and to treat ulcers, snake and scorpion bites. In the present study, we investigated the chemical composition of Chamaecrista diphylla leaves through a mass spectrometry molecular network approach. The oxygen radical absorbance capacity (ORAC) for the ethanolic extract, enriched fractions and isolated compounds was assessed. Overall, thirty-five compounds were annotated for the first time in C. diphylla. Thirty-two of them were reported for the first time in the genus. The isolated compounds 9, 12, 24 and 33 showed an excellent antioxidant capacity, superior to the extract and enriched fractions. Bond dissociation energy calculations were performed to explain and sustain the antioxidant capacity found. According to our results, the leaves of C. diphylla represent a promising source of potent antioxidant compounds.

scholarly journals Biochemical Studies on Phenylhydrazine Induced Experimental Anemic Albino Rats

2015 ◽  
Vol 3 (1) ◽  
pp. 41-47
Author(s):  
Nirjala Laxmi Madhikarmi ◽  
Kora Rudraiah Siddalinga Murthy
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
Enzymatic Antioxidants ◽  
Medical Sciences ◽  
Thiobarbituric Acid Reactive Substances ◽  
Biochemical Studies ◽  
Healthy Control ◽  
Wistar Albino Rats

INTRODUCTION: The present study evaluated the modulatory effects of diphenylhydrazine induced experimental wistar albino rats and also to assess various biochemical parameters in whole blood and red blood cell lysate.MATERIALAND METHODS: Twenty male albino rats weighing 180-200 gm were selected for the study and divided in two groups; ten phenylhydrazine dihydrochloride (PHZ) induced anemia and ten healthy control. Thiobarbituric acid reactive substances and lipid hydroperoxide were measured as lipid peroxidation parameter. The antioxidant vitamins A, C and E and enzymatic antioxidants; catalase, glutathione peroxidase and superoxide dismutase were also assessed.RESULTS: Phenylhydrazine induced anemic rats showed a significant increase in the lipid peroxidation and decrease in the antioxidants as compared to healthy rats.CONCLUSION: The study concludes that phenylhydrazine induced experimental anemic albino rats showed increased oxidative stress than compared with healthy albino rats.Journal of Universal College of Medical Sciences Vol. 3, No. 1, 2015: 41-47 

scholarly journals Doxorubicin Toxicity can be Ameliorated during Antioxidant L-Carnitine Supplementation

2010 ◽  
Vol 3 (6) ◽  
pp. 428-433 ◽  
Author(s):  
Othman A. Alshabanah ◽  
Mohamed M. Hafez ◽  
Mohamed M. Al-Harbi ◽  
Zeinab K. Hassan ◽  
Salim S. Al Rejaie ◽  
...  
Keyword(s):  
Gene Expression ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
Carnitine Supplementation ◽  
Glutathione S Transferase ◽  
Antioxidant Genes ◽  
Expression Levels ◽  
High Cumulative Dose ◽  
Group 2 ◽  
Wistar Albino Rats

Doxorubicin is an antibiotic broadly used in treatment of different types of solid tumors. The present study investigates whether L-carnitine, antioxidant agent, can reduce the hepatic damage induced by doxorubicin. Male Wistar albino rats were divided into six groups: group 1 was intraperitoneal injected with normal saline for 10 consecutive days; group 2, 3 and 4 were injected every other day with doxorubicin (3 mg/kg, i.p.), to obtain treatments with cumulative doses of 6, 12 and 18 mg/kg. The fifth group was injected with L-carnitine (200 mg/kg, i.p.) for 10 consecutive days and the sixth group was received doxorubicin (18 mg/kg) and L-carnitine (200 mg/kg). High cumulative dose of doxorubicin (18 mg/kg) significantly increases the biochemical levels of alanine transaminase, alkaline phosphatase, total bilirubin, thiobarbituric acid reactive substances (TBARs), total nitrate/nitrite (NOx) p < 0.05 and decrease in glutathione (GSH ), superoxide dismutase (SOD), glutathione peroxidase (GSHP x), glutathione-s-transferase (GST), glutathione reductase (GR) and catalase (CAT) activity p < 0.05. The effect of doxorubicin on the activity of antioxidant genes was confirmed by real time PCR in which the expression levels of these genes in liver tissue were significantly decrease compared to control p < 0.05. Interestingly, L-carnitine supplementation completely reversed the biochemical and gene expression levels induced by doxorubicin to the control values. In conclusion, data from this study suggest that the reduction of antioxidant defense during doxorubicin administration resulted in hepatic injury could be prevented by L-carnitine supplementation by decreasing the oxidative stress and preserving both the activity and gene expression level of antioxidant enzymes.

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