scholarly journals Antioxidant activity of bee products added to water in tebuconazole-exposed fish

2012 ◽  
Vol 10 (1) ◽  
pp. 215-220 ◽  
Author(s):  
Daiane Ferreira ◽  
Taís Cristina Unfer ◽  
Hélio Carlos Rocha ◽  
Luiz Carlos Kreutz ◽  
Gessi Koakoski ◽  
...  
Keyword(s):  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
South American ◽  
Enzymatic Antioxidants ◽  
Thiobarbituric Acid Reactive Substances ◽  
Glutathione S Transferase ◽  
Exposed Fish ◽  
Bee Pollen ◽  
Protein Thiols ◽  
Oxidant Effect

An experiment was conducted to evaluate the potential of honey, propolis, and bee pollen for the reversal of lipid peroxidation induced by tebuconazole (TEB) in South American catfish (Rhamdia quelen), in which the concentration of thiobarbituric acid reactive substances (TBARS), the activity of the antioxidant enzyme glutathione-S-transferase (GST) and the concentrations of non-enzymatic antioxidants, reduced glutathione (GSH), ascorbic acid, and non-protein thiols were assessed. Honey (0.125 g L-1) and bee pollen (0.05 g L-1) added to the water reverse the production of TBARS induced by TEB, while propolis demonstrated a pro-oxidant effect, inducing an increase in TBARS production. The data presented herein suggest that the addition of water to honey and bee pollen potentially protects against the oxidative stress caused by agrichemicals.

Antioxidant Activity of 2-Bromo-3-hydroxy-2-nitroproylcinnamte against Alcohol Induced Oxidative Damage

2020 ◽  
Vol 5 (2) ◽  
pp. 156-160
Author(s):  
Rajesh Kumar Malik ◽  
Dharmendra Kumar Singh ◽  
Anuradha ◽  
Surendra Kumar
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Reduced Glutathione ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Alcohol Concentration ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Β Carotene

The present study demonstrated the antioxidant activity of 2-bromo-3-hydroxy-2-nitroproylcinnamte versus alcohol induced oxidative damage in albino wistar rats. In this study, 30 % alcohol exposed rats were found to be more prone to peroxidative risk as they are calculated by species of thiobarbituric acid. It was observed that after the rats induction with 30% alcohol, concentration of lipid peroxidation has been obtained expressively (p ≤ 0.001) high in a liver and serum, beside with concomitant substantial (p ≤ 0.001) reduced in enzymatic and non-enzymatic antioxidants levels like catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), vitamin E (tocopherol), reduced glutathione (GSH), glutathione-s-transferase (GST), vitamin C (ascorbic acid), β-carotene as well as ceruloplasmin in serum along with liver, only 30% alcohol was treated. If rats obtained 2- bromo-3-hydroxy-2-nitroproylcinnamte at the dose level of 20 mg/kg bw/day, PO, for 30 days, the peroxidative damage has been marginal in serum along with liver, alongside efficiently encouraging the potential of antioxidant in the rats treated by alcohol. This study revealed that in liver the raised peroxidative risk is probably allied with alcohol induction pathology that can be decreased by increasing the antioxidant potential by free radical scavenging activity, therefore promising as artificial antioxidants for 2-bromo-3-hydroxy-2-nitroproylcinnamte.

scholarly journals Biochemical Studies on Phenylhydrazine Induced Experimental Anemic Albino Rats

2015 ◽  
Vol 3 (1) ◽  
pp. 41-47
Author(s):  
Nirjala Laxmi Madhikarmi ◽  
Kora Rudraiah Siddalinga Murthy
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
Enzymatic Antioxidants ◽  
Medical Sciences ◽  
Thiobarbituric Acid Reactive Substances ◽  
Biochemical Studies ◽  
Healthy Control ◽  
Wistar Albino Rats

INTRODUCTION: The present study evaluated the modulatory effects of diphenylhydrazine induced experimental wistar albino rats and also to assess various biochemical parameters in whole blood and red blood cell lysate.MATERIALAND METHODS: Twenty male albino rats weighing 180-200 gm were selected for the study and divided in two groups; ten phenylhydrazine dihydrochloride (PHZ) induced anemia and ten healthy control. Thiobarbituric acid reactive substances and lipid hydroperoxide were measured as lipid peroxidation parameter. The antioxidant vitamins A, C and E and enzymatic antioxidants; catalase, glutathione peroxidase and superoxide dismutase were also assessed.RESULTS: Phenylhydrazine induced anemic rats showed a significant increase in the lipid peroxidation and decrease in the antioxidants as compared to healthy rats.CONCLUSION: The study concludes that phenylhydrazine induced experimental anemic albino rats showed increased oxidative stress than compared with healthy albino rats.Journal of Universal College of Medical Sciences Vol. 3, No. 1, 2015: 41-47 

scholarly journals Antioxidant potential of Carica papaya Linn (Caricaceae) leaf extract in mice with cyclophosphamide induced oxidative stress

2020 ◽  
Vol 30 (1) ◽  
pp. e34702
Author(s):  
Tatiane Cordeiro Luiz ◽  
Ana Paula Simões Da Cunha ◽  
Danilo Henrique Aguiar ◽  
Marina Mariko Sugui ◽  
Rogério de Campos Bicudo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carica Papaya ◽  
Hematological Parameters ◽  
Thiobarbituric Acid ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Biochemical Tests ◽  
Red Cell Count ◽  
Phytochemical Profile

AIMS: This study aimed to investigate the effects of crude extract of Carica papaya leaves on oxidative stress in mice induced by cyclophosphamide, as well as phytochemical profile characterization of this extract.METHODS: The male Swiss mice received 15 days of treatment with the extract (500 mg kg-1, via gavage) and intraperitoneal injection of cyclophosphamide (75 mg kg-1) or saline (0.9%) on the 15th day. After 24 h the last treatment, the animals were anesthetized for blood withdrawal, sacrificed and removal of the organs for analyses (liver, kidney and heart). In the biochemical tests were determined: hematological parameters in blood, aminotransferases, alkaline phosphatase, glucose and total cholesterol dosages in plasma, enzymatic and non-enzymatic antioxidants and lipid damage marker were evaluated in different tissues, besides genotoxic and histopathological analyzes.RESULTS: In the extract of Carica papaya leaves, the flavonoids quercetin-3β-D-glucoside and rutin were identified, besides present positive results for alkaloids, saponins and tannins. This extract increased the activity of glutathione-S-transferase and catalase enzymes in the liver and reduced the levels of reduced glutathione in the kidneys and hematocrit levels, red cell count, and hemoglobin. It promoted the decrease of the reactive species of thiobarbituric acid (TBARS) in the kidneys and the activity of enzyme aspartate aminotransferase in the plasma and was antimutagenic in the micronucleus test.CONCLUSIONS: The study showed that extract of Carica papaya was beneficial against oxidative events and prevented DNA damage. The extract also showed hepatotoxicity, therefore prolonged infusion of papaya leaves is not advisable.

scholarly journals Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

2015 ◽  
Vol 62 (2) ◽  
pp. 13-19
Author(s):  
Urmila Jarouliya ◽  
Anish Zacharia ◽  
Raj K. Keservani ◽  
Godavarthi B.K.S Prasad
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

Oxidative damage and antioxidants in cervical cancer

2020 ◽  
pp. ijgc-2020-001587
Author(s):  
Daciele Paola Preci ◽  
Angélica Almeida ◽  
Anne Liss Weiler ◽  
Maria Luiza Mukai Franciosi ◽  
Andréia Machado Cardoso
Keyword(s):  
Cervical Cancer ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Cancer Progression ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
The Impact

The pathogenesis of cervical cancer is related to oxidative damage caused by persistent infection by one of the oncogenic types of human papillomavirus (HPV). This damage comes from oxidative stress, which is the imbalance caused by the increase in reactive oxygen and nitrogen species and impaired antioxidant mechanisms, promoting tumor progression through metabolic processes. The incorporation of HPV into the cellular genome leads to the expression of oncoproteins, which are associated with chronic inflammation and increased production of reactive oxygen species, oxidizing proteins, lipids and DNA. The increase in these parameters is related, in general, to the reduction of circulating levels of enzymatic antioxidants—superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase; and non-enzymatic antioxidants—reduced glutathione, coenzyme Q10 and vitamins A, C and E, according to tumor staging. In contrast, some enzymatic antioxidants suffer upregulation in the tumor tissue as a way of adapting to the oxidative environment generated by themselves, such as glutathione-S-transferase, reduced glutathione, glutathione peroxidase, superoxide dismutase 2, induced nitric oxide synthase, peroxiredoxins 1, 3 and 6, and thioredoxin reductase 2. The decrease in the expression and activity of certain circulatory antioxidants and increasing the redox status of the tumor cells are thus key to cervical carcinoma prognosis. In addition, vitamin deficit is considered a possible modifiable risk factor by supplementation, since the cellular functions can have a protective effect on the development of cervical cancer. In this review, we will discuss the impact of oxidative damage on cervical cancer progression, as well as the main oxidative markers and therapeutic potentialities of antioxidants.

scholarly journals Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

2019 ◽  
Vol 49 (1) ◽  
pp. 41-47
Author(s):  
Ana Paula Simões da CUNHA ◽  
Luana BALDISSERA ◽  
Débora Linsbinski PEREIRA ◽  
Lucineia Reuse ALBIERO ◽  
Lindsey CASTOLDI ◽  
...  
Keyword(s):  
Day Treatment ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Stem Bark ◽  
Protein Carbonylation ◽  
Antioxidant Compounds ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Ehrlich Tumor ◽  
Biochemical Analyses

ABSTRACT Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST)], non-enzymatic antioxidants [reduced glutathione (GSH) and ascorbic acid (ASA)], substances reactive to thiobarbituric acid (TBARS) and protein carbonylation (carbonyl) in different tissues were significantly affected. The extract administered at 200 mg kg-1 presented higher antioxidant capacity in the liver, increased CAT, GST, GSH and decreased TBARS, as well as increased CAT activity and protein carbonylation in brain tissue. The results showed that the copaiba extract was able to reverse the oxidative stress caused by solid Ehrlich tumor, probably due to the presence of antioxidant compounds, and had potential antineoplasic effect after a 30-day treatment.

scholarly journals Evaluation of the antitumor and antioxidant effects of jatobá (Hymenaea courbaril) extracts / Avaliação do efeito antitumoral e antioxidante de extratos do jatobá (Hymenaea courbaril)

2021 ◽  
Vol 7 (12) ◽  
pp. 116001-116018
Author(s):  
Elisângela Miranda de Jesus Lisboa ◽  
Lucinéia Reuse Albiero ◽  
Nadila Melchiors ◽  
Wesley Sandro de Paula Borges ◽  
Valfran da Silva Lima ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Reduced Glutathione ◽  
Tumor Development ◽  
Thiobarbituric Acid ◽  
Renal Tissue ◽  
Ehrlich Carcinoma ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hymenaea Courbaril ◽  
Antioxidant Effects

Ethnobotanical surveys have revealed the use of jatobá for the treatment of several diseases. This study determined the effect of plant extracts on the development of Ehrlich carcinoma. Male Swiss mice (n=6) were subcutaneously inoculated with 106 tumor cells and intragastrically administered ethanol (2 mg·mL-1, 5 mg·mL-1, or 10 mg·mL-1) or aqueous extracts of jatobá seed or bark for 90 days. Tumor development did not significantly differ between the groups studied; however, animals treated with the aqueous extract of the seed (2.205 mg·mL-1) had a reduction in tumor size compared to those treated with the aqueous extract of the bark (1.7 mg·mL-1). The treatment was not found to influence the survival of the animals studied. A new group of animals (n=7), with or without the tumor, received the aqueous extract of jatobá seed for 7, 14, and 30 days to evaluate oxidative stress. The extract reduced the thiobarbituric acid reactive substances levels at 7 days in the liver and kidneys, and 14 days in brain and renal tissue. Protein carbonylation levels were also reduced at 7 days in the liver and brain tissue and 14 days in the liver. The reduced glutathione levels diminished in animals treated for 7 and 14 days. We conclude that treatment with the aqueous extract of the jatobá seed presents promising activity in the reduction of oxidative stress.

scholarly journals Redox parameters in blood of thyroid cancer patients after the radioiodine ablation

2017 ◽  
Vol 32 (4) ◽  
pp. 358-365
Author(s):  
Vera Spasojevic-Tisma ◽  
Milovan Matovic ◽  
Olgica Mihaljevic ◽  
Snezana Zivancevic-Simonovic ◽  
Marija Jeremic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thyroid Cancer ◽  
Cancer Patients ◽  
Differentiated Thyroid Cancer ◽  
Radioactive Iodine ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Radioiodine Ablation

The radioactive iodine (131I) ablation is a well-accepted treatment modality for differentiated thyroid cancer patients. Unfortunately, the radiation induces the oxidative stress and damages cells and tissues, simultaneously activating the mechanisms of antioxidative defense. Since the mechanisms of those processes are not completely known, we wanted to examine the changes in the most important reactive oxygen species and antioxidative components, as well as their correlation and significance for lipid peroxidation. Our results showed that the level of thiobarbituric acid reactive substances was increased during the first 30 days after the radiotherapy. Among antioxidant components, superoxide dismutase was increased in the 3rd and 30th day; catalase in 7th and reduced glutathione in 3rd and 7th day after the radiotherapy. As regards the prooxidants, the reduction of hydrogen peroxide (H2O2) was recorded in 7th and 30th day, and superoxide anion radical (O?-) was unchanged after the exposure to 131I. These results indicate that differentiated thyroid cancer patients are under constant oxidative stress despite the observed increase in antioxidative and reduction in prooxidative parameters. The understanding of these early processes is important since their progress determines the latter effects of 131I therapy.

scholarly journals Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues

2012 ◽  
Vol 84 (4) ◽  
pp. 1121-1126 ◽  
Author(s):  
Seyed M. Nabavi ◽  
Seyed F. Nabavi ◽  
Akbar H. Moghaddam ◽  
William N. Setzer ◽  
Morteza Mirzaei
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Sodium Fluoride ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Glutathione Level ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cardiac Tissues ◽  
And Oxidative Stress

This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals before sodium fluoride consumption modified the levels of biochemical parameters.

scholarly journals Anthocyanins Protect Hepatocytes against CCl4-Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 451 ◽  
Author(s):  
Popović ◽  
Kocić ◽  
Katić ◽  
Zarubica ◽  
Veličković ◽  
...  
Keyword(s):  
Kupffer Cells ◽  
Reduced Glutathione ◽  
Lipid Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Quinone Reductase ◽  
Lipocalin 2 ◽  
Glutathione S Transferase ◽  
Necrosis Factor Alpha ◽  
Anti Inflammatory ◽  
Bilberry Extract

: This study examined the hepatoprotective and anti-inflammatory effects of anthocyanins from Vaccinim myrtillus (bilberry) fruit extract on the acute liver failure caused by carbon tetrachloride-CCl4 (3 mL/kg, i.p.). The preventive treatment of the bilberry extract (200 mg anthocyanins/kg, orally, 7 days) prior to the exposure to the CCl4 resulted in an evident decrease in markers of liver damage (glutamate dehydrogenase, sorbitol dehydrogenase, malate dehydrogenase), and reduced pro-oxidative (conjugated dienes, lipid hydroperoxide, thiobarbituric acid reactive substances, advanced oxidation protein products, NADPH oxidase, hydrogen peroxide, oxidized glutathione), and pro-inflammatory markers (tumor necrosis factor-alpha, interleukin-6, nitrite, myeloperoxidase, inducible nitric oxide synthase, cyclooxygenase-2, CD68, lipocalin-2), and also caused a significant decrease in the dissipation of the liver antioxidative defence capacities (reduced glutathione, glutathione S-transferase, and quinone reductase) in comparison to the results detected in the animals treated with CCl4 exclusively. The administration of the anthocyanins prevented the arginine metabolism’s diversion towards the citrulline, decreased the catabolism of polyamines (the activity of putrescine oxidase and spermine oxidase), and significantly reduced the excessive activation and hyperplasia of the Kupffer cells. There was also an absence of necrosis, in regard to the toxic effect of CCl4 alone. The hepatoprotective mechanisms of bilberry extract are based on the inhibition of pro-oxidative mediators, strong anti-inflammatory properties, inducing of hepatic phase II antioxidant enzymes (glutathione S-transferase, quinone reductase) and reduced glutathione, hypoplasia of Kupffer cells, and a decrease in the catabolism of polyamines.

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