scholarly journals Characterization of the myenteric neuronal population and subpopulation of the duodenum of adult wistar rat fed with hypoproteic chow

2012 ◽  
Vol 84 (3) ◽  
pp. 799-806 ◽  
Author(s):  
Débora M.G. Sant'Ana ◽  
Eduardo J.A. Araújo ◽  
Diego H. Ramos ◽  
Catchia Hermes-Uliana ◽  
Maria Raquel M. Natali
Keyword(s):  
Total Population ◽  
Wistar Rat ◽  
Neuronal Population ◽  
The Body ◽  
Control Group ◽  
Myenteric Neurons ◽  
Lower Body Weight ◽  
Significant Difference ◽  
Rat Duodenum ◽  
Malnourished Group

The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when compared to the control group. Quantitative analysis demonstrated no significant differences between control and malnourished group by using Giemsa; however, as the organ reduction was not followed by an increase inversely proportional to the density of neurons, the condition imposed suggests the loss of neurons from the total population. Nevertheless, through NADPH-d histochemistry, there was a neuronal density increase for the malnourished group. There was no significant difference between the groups for both techniques with respect to the morphometric analysis of the body cell.

scholarly journals Perbedaan Penurunan Suhu Tubuh Tikus Wistar Jantan Dan Betina Yang Mati Akibat Diinduksi Metanol

2021 ◽  
Vol 4 (2) ◽  
pp. 51
Author(s):  
Abdul Hakim Nitiprodjo ◽  
Titik Kusumawinakhyu
Keyword(s):  
Body Temperature ◽  
Wistar Rats ◽  
Wistar Rat ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Male And Female ◽  
Significant Difference ◽  
Post Test ◽  
Female Wistar Rats

Someone who dies will experience a decrease in body temperature from body temperature at the beginning of death, both within normal and abnormal limits to room temperature. Decrease in body temperature according to sex may differ in duration. Coupled with exposure to methanol, it is also possible to influence a decrease in body temperature of the corpse. This study aimed to analyze the differences in body temperature reduction in male and female Wistar rats  induced with methanol.This research is an experimental study with a pre and post test control group design approach. The design of this study was to observe the body temperature of male and female Wistar rats while still alive and after death and the duration of decrease in body temperature of male and female Wistar mice after being induced with methanol. The population studied was male and female Wistar rat. Based on the results from analysis test, it can be concluded that there is no significant difference between the decrease in body temperature of male and female Wistar rats that died induced by methanol, but there is a significant difference between the decrease in body temperature of male and female Wistar rats who died induced by methanol and without methanol.

Effectiveness Of Toilet Training Education On Mother's Behavior And Toddler Age Toilet Training Ability (18-36 Months)

2020 ◽  
Vol 5 (2) ◽  
pp. 49-55
Author(s):  
Hafiko Andresni ◽  
Zahtamal Zahtamal ◽  
Winda Septiani ◽  
Mitra Mitra ◽  
Lita Lita
Keyword(s):  
Sampling Technique ◽  
T Test ◽  
The Body ◽  
Toilet Training ◽  
Wilcoxon Test ◽  
Control Group ◽  
Maternal Knowledge ◽  
Control Group Design ◽  
Significant Difference

ABSTRACT Toilet training is an effort to train children to be able to control and urinate (BAK) and defecate (BAB). Toilet training is one of the main tasks of children at toddler age. Toilet training is one of the main tasks of children in toddler age which is very important to be done to create independence in children in controlling BAK and BAB and children know the parts of the body and their functions. Data in 2012 shows that ± 60% of parents do not teach toilet training to children from an early age. The aim of the study was to find out the effectiveness of toilet training education on maternal behavior and toilet skills in toddler age training (18-36 months). The study was conducted in July-August 2018. This type of quantitative research used the design of the Quasy pretest and posttest experiment with non-equivalent control group design. Samples were 36 mothers and 36 children with purposive sampling technique. Data analysis used Paired t test, Wilcoxon test, Man-Whitney test an Independent t test. The results showed that toilet training education through lecture methods, modules and maze games was more effective than toilet training education through lecture and leaflet methods on children's knowledge and abilities. Conversely, for the role of mothers in supervision there is no significant difference in effectiveness. Health education is recommended in health promotion programs to increase maternal knowledge, the role of mothers and the ability of toilet training children independently. Keywords: Toilet training, Lecture method, Module, Maze game, Leaflet, Knowledge, Role of mother, Children's ability.

A study on the safety evaluation of buphrenorphine administered through an autoinjector compared with manual injection using haematological and biochemical variables in rats

2016 ◽  
Vol 36 (9) ◽  
pp. 901-909 ◽  
Author(s):  
D Sheela ◽  
R Vijayaraghavan ◽  
S Senthilkumar
Keyword(s):  
Body Weight ◽  
Research Work ◽  
Safety Evaluation ◽  
Weight Ratio ◽  
The Body ◽  
Control Group ◽  
Body Weight Ratio ◽  
Significant Difference ◽  
Manual Group ◽  
Significant Change

Buprenorphine drug cartridge was made for autoinjector device for use in emergency and critical situations to reduce the morbidity and mortality. Water-filled cartridges were prepared and buprenorphine was injected aseptically in the cartridge, to make 0.05 and 0.10 mg/mL. Rats were injected intraperitoneally, buprenorphine (0.3 and 0.6 mg/kg), repeatedly with the autoinjector and compared with manual injection (7 days and 14 days) using various haematological and biochemical parameters. No significant change was observed in the body weight, organ to body weight ratio and haematological variables in any of the experimental groups compared with the control group. Except serum urea and aspartate aminotransferase, no significant change was observed in glucose, cholesterol, triglycerides, bilirubin, protein, albumin, creatinine, uric acid, alanine aminotransferase, gamma glutamyltransferase and alkaline phosphatase. The autoinjectors deliver the drugs with spray effect and force for faster absorption. In the present study, the autoinjector meant for intramuscular injection was injected intraperitoneally in rats, and the drug was delivered with force on the vital organs. No significant difference was observed in the autoinjector group compared to the manual group showing tolerability and safety of the buphrenorphine autoinjector. This study shows that buprenorphine autoinjector can be considered for further research work.

Congenital Heart Disease and Childhood Adjustment

1971 ◽  
Vol 2 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Edward T. Auer ◽  
Audrey G. Senturia ◽  
Moisy Shopper ◽  
Ralph L. Biddy
Keyword(s):  
Body Image ◽  
Surgical Intervention ◽  
Intellectual Functioning ◽  
Emotional Disturbances ◽  
The Body ◽  
Psychiatric Clinic ◽  
Control Group ◽  
Significant Difference ◽  
Figure Drawings ◽  
Childhood Adjustment

This report deals with the findings from a study of twenty-eight children, all of whom had ventricular septal defects and were studied by the same cardiovascular team. One-half of the group had a surgical procedure for correction of the defect. The study investigated three problems in children with ventricular septal defect ( VSD). (1) Do children with surgical intervention for VSD show greater impairment of intellectual functioning than non-operated children? (2) Do children with surgical intervention show greater emotional disturbance than non-operated children? (3) Do children with surgical intervention show greater alteration of body image than do non-operated children? Data were collected using questionnaires, family interviews, subject interviews, medical records, school reports, physicians' reports and Human Figure Drawings, both inside and outside the body. The findings conclude that ( a) operated children do demonstrate significantly more impairment of intellectual functioning; ( b) there was no significant difference in the incidence of emotional disturbances between the two groups but that these twenty-eight children with VSD were more similar emotionally to children in a psychiatric clinic group than in a control group; and ( c) the only indication of altered body image was found in the greater frequency with which bones were drawn by the operated group.

scholarly journals Optimalisasi Pertumbuhan Bayi dengan Baby Massage and Spa

2019 ◽  
Vol 10 (2) ◽  
pp. 276
Author(s):  
Yusari Asih ◽  
I Gusti Ayu Mirah WS
Keyword(s):  
Body Length ◽  
Head Circumference ◽  
Intervention Group ◽  
Sampling Technique ◽  
Holistic Approach ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference

<p><span>Baby Massage and Spa was a traditional effort that uses a holistic approach through comprehensive care using a combination of massage and water therapy methods that are carried out in an integrated manner to balance the body, mind, and feelings. Age 3-6 months is the right time for babies to do massage and spa. The purpose of this study was to determine the effect of baby massage and spa on the growth of infants aged 3-5 months in Pringsewu District in 2018. This study uses a quasi-experimental approach with research design pre and post-test with control group design. The purposive sampling technique was taken with a sample of 30 infants as the intervention group and 30 infants as the control group. Data were processed using computerization and analyzed using the T-Test. The results showed that there were significant differences between body weight, body length and baby head circumference in the pre and post-treatment. There was no significant difference in the average increase in infant weight between babies who were carried out by baby massage and spa with babies who were not carried out by baby massage and spa with p-value 0.116. There is a significant difference in the baby's body length and head circumference performed by baby massage and spa with those not carried out by baby massage and spa with p-value 0.000. Baby Massage and Spa has a significant effect on increasing the growth of babies aged 3-5 months.</span></p><p><span> </span></p>

scholarly journals A randomised, open label comparative study of hydroxychloroquine with betamethasone oral mini pulse in the management of patients with alopecia areata

2021 ◽  
Vol 10 (2) ◽  
pp. 187
Author(s):  
Jeyasudha Jambusayee ◽  
Kulur Mukhyaprana Sudha
Keyword(s):  
Alopecia Areata ◽  
Life Quality ◽  
Autoimmune Disorder ◽  
Study Groups ◽  
Pulse Therapy ◽  
The Body ◽  
Control Group ◽  
Study Group ◽  
Open Label ◽  
Significant Difference

Background: Alopecia areata is an autoimmune disorder causing patchy hair loss on scalp and other parts of the body and leading to poor self-esteem and anxiety in patients. Treatment with topical or systemic drugs like steroids or other immunosuppressants is associated with adverse effects. Hydroxychloroquine is an antimalarial drug, with T cell modulating function. This study was undertaken to assess the safety, efficacy and tolerability of Hydroxychloroquine in Alopecia areata compared to betamethasone oral mini pulse (OMP) therapy. Methods: 60 patients with alopecia areata were randomized into two groups of 30 each. Control group received tab. betamethasone 5 mg/day on two consecutive days of week for 12 weeks and Study group received tab. hydroxychloroquine 200 mg/day for 12 weeks. They were followed-up for further 12 weeks. Scale of alopecia tool, dermatology life quality index and global assessment at baseline, 12 weeks and 24 weeks were used to assess the outcome.Results: 94 patients were screened and 60 patients were included. All patients completed the study. At the end of 12 weeks, there was a statistically significant reduction in SALT and DLQI scores in both control and study groups. But at the end of 24 weeks, the study group showed an increase in the scores. Relapses were more in the study group. No significant difference in the incidence of adverse events was noted between the two groups.Conclusions: Hydroxychloroquine 200 mg/day is less efficacious in the management of alopecia areata in comparison to betamethasone oral mini pulse therapy.

scholarly journals Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Leaf Extract ◽  
The Body ◽  
Control Group ◽  
Bb Rat ◽  
Toxic Dose ◽  
Experimental Animals ◽  
Significant Difference ◽  
Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

scholarly journals The effect of ethanol extract of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress

2010 ◽  
Vol 8 (1) ◽  
pp. 11-16
Author(s):  
BAARID LUQMAN HAMIDI ◽  
SAMIGUN SAMIGUN ◽  
ANIK LESTARI
Keyword(s):  
Restraint Stress ◽  
Wistar Rats ◽  
Radial Maze ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Control Group ◽  
Error Type ◽  
Stress Group ◽  
Significant Difference

Hamidi BL, Samigun, Lestari A. 2010. The effect of extract ethanol of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress. Biofarmasi 8: 11-16. The aim of this research was to investigate the effects of extract ethanol of pegagan (Centella asiatica) after treated with restraint stress by measuring the eight arms radial maze performance of rats. Pre-test and post-test controlled groups design was applied in this research. Male Wistar rats (Rattus novergicus) with the mean age of 8 weeks and the body weight of 150-200 grams which used for sample were divided randomly into 4 groups, each group consisted of 6 rats, i.e. (i) control group (without any treatment), (ii) stress group (it was given by restraint stress for 2 hours/day for each rat), (iii) pegagan group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan), and (iv) pegagan and stress group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan and restraint stress for 2 hours/day for each rat). The treatments were given for 21 days. Within 12 days for each pre-treatment and post-treatment, a test on the eight arm radial maze was conducted on individual rat to observe its performance. The assessment of rat performance in the eight arms radial maze test was conducted based on error type B. Kruskal-Wallis and Mann-Whitney tests with SPSS for Windows 16 version were applied to analyze statistically the difference between four groups. Kruskal-Wallis test was used to show the significant performance level difference between four groups of rats with p=0.001, while Mann-Whitney test was used to determine the significant difference between stress group and pegagan group (p=0.001), also stress group and pegagan and stress group (p=0.001). The result of research showed that there was no significant difference between control group and stress group (p=0.051), control group and pegagan group (p=0.143), control group and pegagan and stress group (p=0.143), also pegagan group and pegagan and stress group (p=0.952). It was concluded that extract ethanol of pegagan improved the performances of rats on the eight arms radial maze after treated with restraint stress.

PENGARUH MENGKONSUMSI GULA AREN (Arenga pinnata) SEBELUM OLAHRAGA TERHADAP DAYA TAHAN OTOT

2019 ◽  
Vol 1 (1) ◽  
pp. 20
Author(s):  
Cecep Muhammad Alawi ◽  
Hamidie Ronald Daniel Ray
Keyword(s):  
Physical Exercise ◽  
Significant Influence ◽  
The Body ◽  
Control Group ◽  
Muscular Endurance ◽  
Control Group Design ◽  
Squat Jump ◽  
Jump Test ◽  
Significant Difference ◽  
Palm Sugar

Two kinds of carbohydrates that are used with the body as energy, is blood glucose and muscular glycogen. Savings glycogen in muscular is one of the factors that is affecting muscular endurance when physical exercise. The aim of this study is to find out the significant influence of consuming palm sugar to muscular endurance before physical exercise. The method that is used in this study is to experiment with design pretest-posttest control group design. Total of sample of this study is 18 trained mans that is divided into three groups. The first experiment group is given palm sugar that is dissolved in water, the second experiment group is given sugar that is dissolved in water, and the control group is given mineral water. The instrument of this study is used push up test, sit up test, and squat jump test to measures the upper muscular endurance, middle, and bottom. All of the samples have done pretest and posttest in different day. When posttest, all of the sample is consumed beverage based on the treatment 30 minutes before do the posttest. Data analysis by Paired Sample t-test and continued by One Way ANOVA to see the influence and comparison between groups. The result of this study has showed that there is a significant difference, the raising of muscular endurance is proved by the score (sig) 0.00>0.05, which the highest increasing is the palm sugar group. Therefore, it could be concluded that there is a significant influence of consuming palm sugar to muscular endurance before physical exercise.

scholarly journals Experimental and Preliminary Clinical Study of Real-Time Registration in Liver Tumors During Respiratory Motion Based on a Multimodality Image Navigation System

2019 ◽  
Vol 18 ◽  
pp. 153303381985776
Author(s):  
Chao Ren ◽  
Shi-rong Liu ◽  
Wen-bo Wu ◽  
Xiao-ling Yu ◽  
Zhi-gang Cheng ◽  
...  
Keyword(s):  
Clinical Study ◽  
Real Time ◽  
Respiratory Motion ◽  
Liver Tumors ◽  
The Body ◽  
Respiratory Monitoring ◽  
Control Group ◽  
Registration Errors ◽  
Significant Difference ◽  
Monitoring Module

Purpose: To develop a fusion imaging system that combines ultrasound and computed tomography for real-time tumor tracking and to validate the accuracy of performing registration via this approach during a specific breathing phase. Materials and Methods: The initial part of the experimental study was performed using iodized oil injection in pig livers and was focused on determining the accuracy of registration. Eight points (A1-4 and B1-4) at different positions and with different target sizes were selected as target points. During respiratory motion, we used our self-designed system to perform the procedure either with (experimental group, E) or without (control group, C) the respiratory monitoring module. The registration errors were then compared between the 2 groups and within group E. The second part of this study was designed as a preliminary clinical study and was performed in 18 patients. Screening was performed to determine the combination of points on the body surface that provided the highest sensitivity to respiratory motion. Registration was performed either with (group E) or without (group C) the respiratory monitoring module. Registration errors were compared between the 2 groups. Results: In part 1 of this study, there were fewer registration errors at each point in group E than at the corresponding points in group C ( P < .01). In group E, there were more registration errors at points A1 and B1 than at the other points ( P < .05). There was no significant difference in registration errors among the remaining points. During part 2 of the study, there was a significant difference in the registration errors between the 2 groups ( P < .01). Conclusions: Real-time fusion registration is feasible and can be accurately performed during respiratory motions when using this system.

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