scholarly journals Early morphological and histochemical alterations in rats subjected to ileocystoplasty

2009 ◽  
Vol 24 (5) ◽  
pp. 393-399 ◽  
Author(s):  
Edinaldo Gonçalves de Miranda ◽  
Marcelo Ribeiro de Sousa Bizerra ◽  
Daniel Reis Waisberg ◽  
José Carnevale ◽  
José Figueredo Silva ◽  
...  
Keyword(s):  
Chronic Inflammation ◽  
Muscular Atrophy ◽  
Statistical Significance ◽  
Squamous Metaplasia ◽  
Anastomosis Group ◽  
Group Iv ◽  
Female Rats ◽  
Inflammatory Infiltration ◽  
Group Iii ◽  
Group I

PURPOSE: To study morphologic and histochemical alterations arising at the ileocystoplasty site. METHODS: Sixteen Wistar female rats were subjected to ileocystoplasty and sacrificed after eight weeks. Material collected was divided into four groups for histological and histochemical studies: Group I (control) - isolated ileum segment removed during ileocystoplasty; Group II - ileoileal anastomosis; Group III - ileovesical anastomosis and Group IV - ileal segment from the neobladder. Histological and histochemical study assessed dysplasia, metaplasia, acute and chronic inflammation, fibrosis, atrophy, hypertrophy, total mucins, sialomucins and sulfomucins. The non-parametric Wilcoxon and Mann-Whitney tests were employed in statistical analysis. RESULTS: None of the groups presented dysplasia. Acute inflammation and atrophy occurred in Groups II, III and IV, not reaching statistical significance. Metaplasia was significant only in Group III (p=0.012). Chronic inflammation, fibrosis and hypertrophy were significant in Groups II, III and IV. There was a significant increase in total mucin content in Group IV (p=0.014) and a reduction in Group III (p=0.016). Increases in sialomucins were observed in samples for Groups III (p=0.003) and IV (p=0.002) along with reduced sulfomucins in samples from Groups III (p=0.013) and IV (p=0.008). CONCLUSION: Ileocystoplasty in female rats caused squamous metaplasia, chronic inflammatory infiltration, fibrosis, hypertrophy, increase in sialomucin content, reduction in sulfomucins, and alterations in total mucin content with statistical significance, as well acute inflammatory infiltration and muscular atrophy with less intensity.

Nitrogen-fixing communities in an intertidal ecosystem

1978 ◽  
Vol 24 (8) ◽  
pp. 932-938 ◽  
Author(s):  
B. Ben Bohlool ◽  
William J. Wiebe
Keyword(s):  
Nitrogen Fixation ◽  
Photosynthetic Bacteria ◽  
Statistical Significance ◽  
Group Iv ◽  
Group Iii ◽  
Group I ◽  
Mud Flat ◽  
Intertidal Ecosystem ◽  
The Mean

Nitrogen fixation (acetylene reduction) associated with various communities in the intertidal sand and mud flat was measured in situ. Areas which were colonized by algae, plants, and animals generally had significantly higher activities than areas which were visibly uncolonized. The highest activities were measured at sites colonized by a mixed bloom of Oscillatoria–Euglena–photosynthetic bacteria. These areas occupied only about 1% of the surface of the sediments, yet contributed nearly 50% of the biologically fixed nitrogen. Enteromorpha communities also exhibited relatively high activities. Sites were grouped according to the statistical significance of the mean of their activities: group I: uncolonized sand, mud and gravel flats, Eelgrass (Zostera) communities, and snail (Amphibola) beds, with an estimated contribution of 1 g N ha−1 day−1; group II: Cockel (Chione) beds, 3g N ha−1 day−1; group III: Salicornia, Ulva, Juncus, and Enteromorpha, 10 g N ha−1 day−1; and group IV: Oscillatoria–Euglena–photosynthetic bacteria association, 200 g N ha−1 day−1. Enteromorpha and Oscillatoria–Euglena–photosynthetic bacteria communities showed significantly lower rates of nitrogen fixation in the dark than in the light.

scholarly journals Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

2016 ◽  
Vol 43 (2) ◽  
pp. 80-86 ◽  
Author(s):  
Conceição Aparecida Dornelas ◽  
Alessandra Marques dos Santos ◽  
Antonio Lucas Oliveira Correia ◽  
Camila de Carvalho Juanes ◽  
João Paulo Ferreira Coelho ◽  
...  
Keyword(s):  
Squamous Metaplasia ◽  
Transitional Cell ◽  
Group Iv ◽  
Aberrant Crypt Foci ◽  
Control Group ◽  
Group Iii ◽  
Intestinal Epithelia ◽  
Bladder Carcinogenesis ◽  
Group I ◽  
Transitional Cells

ABSTRACT Objective: to evaluate the effect of L-lysine in the bladder and intestinal epithelia in rats submitted to vesicosigmoidostomy. Methods: we divided forty Wistar rats into four groups: group I - control group (Sham); group II - submitted to vesicosigmoidostomy and treated with L-lysine 150mg/kg; group III - submitted only to vesicosigmoidostomy; and group IV - received L-lysine 150mg/kg. After eight weeks the animals were sacrificed. Results: in the bladders of all operated animals we observed simple, papillary and nodular hyperplasia of transitional cells, transitional cell papillomas and squamous metaplasia. As for the occurrence of aberrant crypt foci in the colons of operated animals, we did not observe statistically significant differences in any of the distal, proximal and medium fragments, or in all fragments together (p=1.0000). Conclusion: Although statistically there was no promotion of carcinogenesis in the epithelia of rats treated with L-lysine in the observed time, it was clear the histogenesis of bladder carcinogenesis in its initial phase in all operated rats, this being probably associated with chronic infection and tiny bladder stones.

scholarly journals Smoking influences on the thickness of marginal gingival epithelium

2003 ◽  
Vol 17 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Cristina Cunha Villar ◽  
Antonio Fernando Martorelli de Lima
Keyword(s):  
Statistical Significance ◽  
Clinical Signs ◽  
Group Iv ◽  
Oral Epithelium ◽  
Group Iii ◽  
Epithelial Thickness ◽  
Gingival Health ◽  
Group I ◽  
Gingival Epithelium ◽  
Health Situation

Smoking patients show reduction of inflammatory clinical signs that might be associated with local vasoconstriction and an increased gingival epithelial thickness. The purpose of this work was to evaluate the thickness of the marginal gingival oral epithelium in smokers and non-smokers, with clinically healthy gingivae or with gingivitis. Twenty biopsies were obtained from four different groups. Group I: non-smokers with clinically healthy gingivae (n = 5). Group II: non-smokers with gingivitis (n = 5). Group III: smokers with clinically healthy gingivae (n = 5). Group IV: smokers with gingivitis (n = 5). These biopsies were histologically processed, serially sectioned at 5 mm, stained with H. E., and examined by image analysis software (KS400), which was used to perform the morphometric evaluation and the quantification of the major epithelial thickness, the epithelial base thickness and the external and internal epithelial perimeters. Differences between the four groups were analyzed using ANOVA test and Tukey's test. The criteria for statistical significance were accepted at the probability level p < 0.05. A greater epithelial thickness was observed in smokers independent of the gingival health situation.

scholarly journals The effect of different levels of organic selenium on broiler slaughter traits

2009 ◽  
Vol 25 (1-2) ◽  
pp. 23-34 ◽  
Author(s):  
Zivan Jokic ◽  
Z. Pavlovski ◽  
S. Mitrovic ◽  
V. Djermanovic
Keyword(s):  
Statistical Significance ◽  
Group Iv ◽  
Breast Mass ◽  
Group Iii ◽  
Organic Selenium ◽  
Group I ◽  
Average Share ◽  
Group Ii ◽  
Different Levels ◽  
Selenized Yeast

The effect of different levels of organic selenium (selenized yeast) on slaughter meat traits of fattening chickens (broilers) was investigated. Trial was carried out on 120 Hybro-PN chickens divided into four groups, in duration of six weeks. All groups of chickens were fed complete mixtures, and selenium was added to the food in the form of selenized yeast in the amount of 0 (I ); 0,3 (II); 0,6 (III) or 0,9mg/kg (IV-group). The highest average values of carcass yield 'conventional processing', 'ready to roast' and 'ready to grill' were recorded in chickens of group III (2059,6, 1851,97 and 1756,45g), with addition of 0,6mg Se/kg, followed by chickens of group II (2048,17, 1841,36 and 1748,57g) and group IV (1957,46, 1764,22 and 1676,05g) with 0,3 and 0,9mg/kg of added selenium, respectively, whereas the lowest values (1835,21, 1660,87 and 1572,61g) were established in chickens of group I without addition of this micro element. Average values of slaughter yields 'conventional processing', 'ready to roast' and 'ready to grill' were approximately the same in chickens of all investigated groups, and established differences showed no statistical significance (P > 0,05). The lowest breast mass was in I group (573,55g), and the highest in group III (657g). Chickens of groups II and IV (with 0,3 and 0,9mg Se/kg of feed) realized breast mass of 627,90 and 633,12g. Compared to group I, average breast mass of chickens in groups III and IV was considerably higher (P < 0,05 and P < 0,01). Share of breast in the mass of processed carcass was the highest in groups III and IV (37,40 and 37,86%), and the lowest in group II (36,0%). In chickens of group I the stated value was 36,47%. Average share of thighs in the mass of processed carcass was approximately the same in all investigated groups. The highest value (14,4%) was established in chickens of group II, and the lowest (13,98%), in chickens of III investigation group. Chickens fed organic selenium in diet (0,3, 0,6 and 0,9mg/kg) had by 3,80, 3,74 and 3,18% higher share of drumsticks in the mass of processed carcass compared to group I. .

scholarly journals Histopathological and histomicrobiological study of root canal therapy medication, comparison of calcium hydroxide versus gutta-percha with zinc oxide/eugenol in the teeth of dogs

2007 ◽  
Vol 27 (2) ◽  
pp. 71-74
Author(s):  
Léslie M. Domingues-Falqueiro ◽  
Marco A. Gioso ◽  
João G. Padilha Filho
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Calcium Hydroxide ◽  
Statistical Significance ◽  
Inflammatory Infiltration ◽  
Group Iii ◽  
Root Canals ◽  
Gutta Percha ◽  
Zinc Oxide Eugenol ◽  
Group I

The presence of microorganisms in dental structures with experimentally induced necrosis was evaluated. The materials were tested to evaluate their antimicrobial activity and tissue repair efficacy. Four dogs were used in this experiment, with a total of 64 roots of premolar teeth, divided into three groups. The root canals of Group I were filled with gutta-percha and zinc oxide/eugenol cement; Group II were filled with calcium hydroxide, and Group III were not filled. All animals were clinically and radiographically examined 15 days after surgery andthen again every subsequent 15 days until 120 days, when the teeth were extracted en bloc.Histopathological analysis showed inflammatory infiltration, cement and bone resorption andnecrotic tissue in the apical delta in different proportions. Histomicrobiological analysis showedthe presence of microorganisms inside the teeth structures, with different concentrationsaccording to the treatment used. There was statistical significance between the groups(p>0.05). Gutta-percha with zinc oxide/eugenol demonstrated good antimicrobial activity;calcium hydroxide was not efficient. The conclusion of this study is that gutta-percha withzinc oxide/eugenol is the better protocol for filling root canals in dogs.

scholarly journals Effectiveness of Soursop Leaf Extract (Annona muricata l.) on IL-6 Levels in Mammary Sprague dawley Female Rats Induced by Staphylococcus aureus

2020 ◽  
Vol 9 (1) ◽  
pp. 56-63
Author(s):  
Feby Purnamasari ◽  
Risfah Yulianty ◽  
Syamsa Latief
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Group Iv ◽  
Female Rats ◽  
Annona Muricata ◽  
Sprague Dawley ◽  
Positive Bacterium ◽  
Group Iii ◽  
Group I ◽  
Nursing Mothers

Staphylococcus aureus is a Gram-positive bacterium that causes inflammation such as mastitis in the breast. Breast mastitis can occur in nursing mothers and requires first aid or initial treatment. Herbal ingredients can be utilized because they are easily obtained and can be prepared by the community themselves. The study aimed to analyze the effectiveness of soursop leaf extract on IL-6 levels in mammae of female rats Sprague dawley induced by S. aureus. The study was divided into four groups consisting of five rats that were induced by S. aureus 2 x 107 CFU/ml. Group I was given 0.5% Na.CMC, Group II gave 45 mg/kgBW cefadroxil antibiotics, group III administered 100 mg / kgBW soursop leaf extract, and group IV administered soursop leaf extract combined with cefadroxil. Each group was given treatment twice a day for 5 days. IL-6 levels were measured on days 3 and 6. The results of this study showed IL-6 levels in group III were 7.50 ± 1.74 pg/ml on the 3rd day, and 4.51 ± 1.47 pg/ml on the 6th day. The results showed there were differences in IL-6 levels in group III (p = 0.037). Soursop leaf extract can be used as a therapy for mastitis caused by S. aureus.

Analysis of surface conditioning methods on core-veneer bond strength of CAD/CAM zirconia restorations

2020 ◽  
pp. 1-11
Author(s):  
Anip K. Roy ◽  
Govind N. Prasad ◽  
Tushar V. Bhagat ◽  
Saurabh Chaturvedi ◽  
Vishwanath Gurumurthy ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Surface Conditioning ◽  
Group I ◽  
Alumina Particles ◽  
Scanning Electron

BACKGROUND: The increased strength of zirconia has resulted in its widespread application in clinical dentistry. Nevertheless, the fracture of veneering porcelains remains one of the key reasons of failure. OBJECTIVE: The objective of this study was to compare and analyze the influence of surface conditioning methods on the core-veneer bond strength of zirconia restorations. METHODS: Thirty specimens of zirconia core with sizes 10 × 5 × 5 mm were layered with porcelain of sizes 5 × 3 × 3 mm. On the basis of different surface conditioning methods, four groups were made: Group I: abrasion with airborne alumina particles of 110 μm size, Group II: sandblasting with silica coated alumina particles of 50 μm in size, Group III (modified group): alteration with a coating of zirconia powder prior to sintering, and Group IV (control group): metal core specimens. The shear force of all specimens was tested using a universal testing machine with a 0.5 mm/min crosshead speed. One-way analysis of variance (ANOVA) and Tukey’s post hoc pair wise comparison (p= 0.05) were performed to analyze the shear bond strength. A scanning electron microscope was used to assess the fractured specimens. RESULTS: A statistically significant difference was noted between the groups. The mean value of shear bond strength was 40.25 MPa for Group I, 41.93 MPa for Group II, 48.08 MPa for Group III and 47.01 MPa for Group IV. CONCLUSIONS: The modified zirconia group and control group demonstrated a significantly higher mean bond strength than that of Group I, where airborne particle abrasion was used. The scanning electron microscope showed that cohesive fracture in the porcelain veneers was the main problem of failure in altered zirconia. The modified zirconia specimens in Group III demonstrated significantly improved values of shear bond strength.

scholarly journals Effect and mechanisms of reproductive tract infection on oxidative stress parameters, sperm DNA fragmentation, and semen quality in infertile males

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Kang-Sheng Liu ◽  
Xiao-Dong Mao ◽  
Feng Pan ◽  
Rui Fang An
Keyword(s):  
Reproductive Medicine ◽  
Reproductive Tract ◽  
Group Iv ◽  
Semen Parameters ◽  
Group Iii ◽  
Reproductive Tract Infection ◽  
Group I ◽  
Sperm Dna ◽  
Group Ii ◽  
Elastase Level

AbstractRecent years have seen a rising incidence of male infertility, mostly caused by the decline of sperm quality. The ratio of infertile males to infertile females has escalated from 3:7 in 2013 to current 5:5, which turns male infertility into the research focus of reproductive medicine. This study aimed to clarify the effect of reproductive tract infection by ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) on the DNA integrity and routine semen parameters of infertile males. A retrospective study was performed. A total of 259 infertile males who were treated at the Andrological Laboratory Examination and Reproductive Medicine Center in our hospital were analyzed. qRT-PCR was used to examine the infection status of CT and UU. According to the eligibility criteria, we evaluated the semen parameters and biochemical data of 253 men. Based on the results of PCR, the subjects were divided into four groups: Group I (CT positive, 63 cases), Group II (UU positive, 60 cases), Group III (CT positive and UU positive, 62 cases), and Group IV (no infection, 68 cases). DNA fragmentation index (DFI), sperm count, vitality and morphology, elastase level, seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Compared to Group IV, three groups (Group I, Group II and Group III) showed difference in semen volume, proportion of sperm with normal morphology, sperm motility, progressive motility, and vitality (P < 0.05). Compared to Group IV, Group II and Group III showed difference in DFI (P < 0.05). Compared to Group IV, Group II and Group III showed difference in elastase level (P < 0.05). VCL, VSL, VAP, WOB, ROS, TM, HDS showed differences between groups of abnormal/normal WBC (*P < 0.01).UU infection significantly increased the level of seminal leukocytes only in Group II, but not in the other three groups, indicating that UU is a factor to increase the level of seminal leukocytes. Compared with the normal leukocyte group, there were significant differences in total motility, forward motility and normal sperm ratio between the two groups. The proportion of sperm with abnormal morphology (mostly in the head) showed obvious difference between groups of high and normal seminal leukocytic levels. At the same time, in this study, SCGE and SCD verified that leukocytes could damage sperm DNA by increasing ROS, which ultimately affects male fertility.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

Effect of amino acid infusion on renal hemodynamics in humans: a dose-response study

1994 ◽  
Vol 267 (5) ◽  
pp. F703-F708 ◽  
Author(s):  
M. Giordano ◽  
P. Castellino ◽  
E. L. McConnell ◽  
R. A. DeFronzo
Keyword(s):  
Amino Acid ◽  
Dose Response ◽  
Renal Hemodynamics ◽  
Group Iv ◽  
Group V ◽  
Group Iii ◽  
Amino Acid Infusion ◽  
Group I ◽  
Basal Plasma ◽  
Group Ii

We evaluated the dose-response relationship between the plasma amino acid (AA) concentration and renal hemodynamics in eight normal subjects. After an overnight fast, a balanced 10% AA solution was infused for 180 min at five separate infusion rates: 0.5 (group I), 1.0 (group II), 2.0 (group III), 4.0 (group IV), and 6.0 (group V) ml.kg-1.min-1 on separate days. Basal plasma AA concentration was 1.87 +/- 0.1 mmol/l and increased to 2.26 +/- 0.1 (group I), 2.66 +/- 0.2 (group II), 3.79 +/- 0.5 (group III), 5.81 +/- 0.4 (group IV), and 7.41 +/- 0.4 mmol/l (group V). Basal glomerular filtration rate (GFR) and renal plasma flow (RPF) averaged 95 +/- 4 and 476 +/- 29 ml.1.73 m-2.min-1, respectively, and rose to 98 +/- 5 and 506 +/- 40 (group I) [P = not significant (NS)], 102 +/- 3 and 533 +/- 30 (group II) (P < 0.05 vs. basal), 110 +/- 4 and 567 +/- 29 (group III), 115 +/- 7 and 610 +/- 55 (group IV), and 117 +/- 7 and 614 +/- 66 ml.1.73 m-2.min-1 (group V) (P = NS vs. group IV). Basal plasma glucagon concentration averaged 68 +/- 10 pg/ml and increased to 74 +/- 10 (group I), 83 +/- 11 (group II) (P < 0.05 vs. basal), 100 +/- 14 (group III), 121 +/- 14 (group IV), and 229 +/- 35 pg/ml (group V) (P < 0.01 vs. basal). Increases in plasma growth hormone (GH) and insulin levels were observed only during groups IV and V.(ABSTRACT TRUNCATED AT 250 WORDS)

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