scholarly journals Synergistic suppression of pre-perfusion of donor livers with recipient serum and cobra venom factor treatment on hyperacute rejection following liver xenotransplantation

2012 ◽  
Vol 27 (5) ◽  
pp. 301-305 ◽  
Author(s):  
Baohua Zhu ◽  
Chuanming Tong ◽  
Weitao Guo ◽  
Rong Pu ◽  
Guoping Zhang ◽  
...  
Keyword(s):  
Survival Time ◽  
Hyperacute Rejection ◽  
Cobra Venom ◽  
Control Group ◽  
Sprague Dawley ◽  
Donor Liver ◽  
Cobra Venom Factor ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
And Control

PURPOSE: To investigate synergistic suppression of donor liver pre-perfusion with recipient serum (RS) and cobra venom factor (CVF) treatment on hyperacute rejection (HAR) following liver xenotransplantation. METHODS: Guinea-pigs (GP, n=24) and Sprague-Dawley rats (SD, n=24) were recruited. Before transplantation, serum was collected from SD rats and used for preparation of inactivated complements. GP and SD rats were randomly assigned into four groups (n=6), respectively: RS group, CVF group, RS+CVF group and control group. Orthotopic liver xenotransplantation was performed with modified two-cuff technique. The survival time and liver function of recipients, morphological and pathological changes in rat livers were investigated. RESULTS: There was no piebald like change in the recipient livers in all experiment groups. The survival time of recipients in all experiment groups was longer than that in control group (p<0.05). Moreover, the survival time in the RS+CVF group was markedly longer than that in the RS group (p<0.01) and CVF group (p<0.05). The serum ALT level in all experiment groups were lower than that in the control group (p<0.05). Furthermore, the ALT level in the RS+CVF group was significantly lower than that in the CVF group (p<0.05) and RS group (p<0.01). The histological damages were significantly improved when compared with the control group, and the histological damages in the RS+CVF group were milder than those in the remaining groups (p<0.05) CONCLUSION: Pre-perfusion of donor liver with recipient serum and cobra venom factor treatment can exert synergistic suppressive effects on the hyperacute rejection following liver xenotransplantation.

scholarly journals Experimental Study of the Effects of Hypoxia Simulator on Osteointegration of Titanium Prosthesis in Osteoporotic Rats

2021 ◽  
Author(s):  
Jiangfeng Liu ◽  
Huijun Kang ◽  
Jiangfeng Lu ◽  
Yike Dai ◽  
Fei Wang
Keyword(s):  
Control Group ◽  
Osteoporotic Bone ◽  
Mrna Levels ◽  
Micro Ct ◽  
Sprague Dawley ◽  
Pull Out ◽  
Sprague Dawley Rats ◽  
Bone To Implant Contact ◽  
Polymerase Chain ◽  
And Control

Abstract Purpose: Poor osseointegration is the key reason for implant failure after arthroplasty, whether in osteoporotic or normal bone conditions. To date, osseointegration remains a major challenge. Recent studies have shown that deferoxamine(DFO) can accelerate osteogenesis by activation of the hypoxia signal pathway. The purpose of this study is to test the following hypothesis: after knee replacement, intra-articular injection of DFO will promote osteogenesis and osseointegration with titanium prosthesis in the bones of osteoporotic rats.Materials and Methods: 90 female sprague-dawley rats were used for the experiment. Ovariectomy and knee arthroplasty were performed. Then, the rats were randomly divided into DFO and control group(n=40 per group). The two groups were treated by intraarticular injection of DFO and saline respectively. After 2 weeks, polymerase chain reaction(PCR) and immunohistochemistry were used to evaluate the levels of HIF-1a, VEGF and CD31. After 12 weeks, the specimens were examined by micro CT, biomechanics and histopathology to evaluate osteogenesis and osseointegration.Results: The results of PCR showed mRNA levels of VEGF and CD31 in DFO group were significantly higher than those in control group. The immunohistochemistry results indicated positive cell expressions of HIF-1a, VEGF and CD31 in DFO group were also higher. Compared to control group, the microCT parameters of BMD, BV/TV, TB.N, TB.Th were significantly higher. The maximal pull-out force and the bone-to-implant contact (BIC) value were also higher . Conclusions: The local administration of DFO which is used to activate HIF-1a signaling pathway can promote osteogenesis and osseointegration with the prosthesis in osteoporotic bone.

scholarly journals THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION, CLIMBING AND COITUS FREQUENCIES IN MALE SPRAGUE-DAWLEY RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 33-40
Author(s):  
Nuning Rahmawati ◽  
Ika Yanti Marfuatush Sholikhah
Keyword(s):  
Treatment Group ◽  
Antibacterial Effect ◽  
Centella Asiatica ◽  
Control Group ◽  
Sprague Dawley ◽  
Pharmacological Activities ◽  
Testosterone Levels ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Curcuma Xanthorrhiza

ABSTRACT Talinum paniculatum (som Java) reported to have many pharmacological activities such as increasing spermatozoa quality, cell regenerations, fertility, and antibacterial effect both empirically and scientifically. This study aimed to prove the combination infusion effects of som Java, gotu kola, and temulawak on introduction, climbing, and coitus levels as well as testosterone levels in male Sprague Dawley (SD) rats. SD rats were divided into 2 groups, control group received aquadest, treatment group received formula a mixture of som java Talinum paniculatum) root, gotu kola (Centella asiatica) herb, and Javanese turmeric (Curcuma xanthorrhiza) rhizome with the dose of 491,2 mg/200 g BW for 7 consecutive days. Artificial estrus of female SD rats were induced by administering estradiol valerat 2 mg/200 g BW 48 hours before observation. The determined parameters were levels of testosterone as well as introduction, climbing and coitus frequencies. Administration of estradiol valerat led to artificial estrus induction of female SD rats. The administration of the combination infusion dose 491.2 mg/200 g BW significantly increased testosterone level as well as introduction and climbing frequencies of male SD rats as of 27.77%; 86.39% and 69.17% respectively. ABSTRAK Som jawa (Talinum paniculatum) secara empiris dan ilmiah terbukti telah banyak dimanfaatkan oleh masyarakat untuk mengatasi berbagai masalah kesehatan diantaranya untuk meningkatkan kualitas dan kuantitas spermatozoa, regenerasi sel, fertilitas, dan sebagai antibakteri. Penelitian ini dilakukan untuk membuktikan efek peningkatan kadar hormon testosteron serta frekuensi introduction, climbing, dan coitus tikus jantan SD dari pengaruh infusa kombinasi som jawa, pegagan, dan temulawak. Hewan uji tikus galur SD dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan akuades, sedangkan kelompok perlakuan diberikan infusa ramuan akar som jawa, herba pegagan, dan rimpang temulawak dosis 491,2 mg/200 g bb selama 7 hari berturut-turut. Induksi estrus artifisial tikus betina dengan pemberian estradiol valerat 2 mg/200 g bb dilakukan 48 jam sebelum pengamatan. Parameter yang diukur meliputi kadar testosterone, frekuensi introduction, climbing, dan coitus. Dibandingkan dengan kelompok kontrol, pemberian infusa ramuan tidak berefek pada frekunsi coitus, namun mampu meningkatkan kadar testosteron serta frekuensi introduction dan climbing tikus jantan sebesar 27,77; 86,39 dan 69,17%.          

scholarly journals The Effects of L-Arginine on Liver Damage in Experimental Acute Cholestasis an Immunohistochemical Study

HPB Surgery ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Yucel Ozsoy ◽  
Mustafa Ozsoy ◽  
Teoman Coskun ◽  
Kemal Namlı ◽  
Ahmet Var ◽  
...  
Keyword(s):  
Liver Injury ◽  
Liver Damage ◽  
Biliary Obstruction ◽  
Hepatic Tissue ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Duct Ligation ◽  
And Control ◽  
Acute Cholestasis

Obstructive jaundice damages critical functions in the liver. Nitric oxide modulation would influence liver damage induced by biliary obstruction, and little is known about it Acute cholestasis was induced by bile duct ligation (BDL) in two groups of male Sprague-Dawley rats. L-Arginine or serum physiologic was administered to treatment and control group. Histopathological and immunohistochemical iNOS expression was investigated in hepatic tissue. Plasma enzyme activities were increased in acute cholestasis, and that L-arginine treatment partially but significantly prevented the elevation of these markers of liver damage (P< .05). Also histopathology scoring showed that the liver injury was prevented and immunohistochemical iNOS activity was increased significantly in L-arginine group (P< .05). This study shows that, after 7 days of biliary obstruction, liver damage is well established and exogenous L-arginine treatment partially but significantly prevented the liver injury in acute cholestasis.

scholarly journals Possible Association Between DHEA and PKCε in Hepatic Encephalopathy Amelioration: A Pilot Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Alessandro Di Cerbo ◽  
Luca Roncati ◽  
Carlotta Marini ◽  
Gianluca Carnevale ◽  
Manuela Zavatti ◽  
...  
Keyword(s):  
Hepatic Encephalopathy ◽  
Neuroprotective Effect ◽  
Brain Area ◽  
Control Group ◽  
Sprague Dawley ◽  
Kinase C ◽  
Sprague Dawley Rats ◽  
Protein Kinase C Epsilon ◽  
Neuronal Functions ◽  
And Control

Objective: Hepatic encephalopathy (HE) is a neuropsychiatric syndrome caused by liver failure and by an impaired neurotransmission and neurological function caused by hyperammonemia (HA). HE, in turn, decreases the phosphorylation of protein kinase C epsilon (PKCε), contributing to the impairment of neuronal functions. Dehydroepiandrosterone (DHEA) exerts a neuroprotective effect by increasing the GABAergic tone through GABAA receptor stimulation. Therefore, we investigated the protective effect of DHEA in an animal model of HE, and the possible modulation of PKCε expression in different brain area.Methods: Fulminant hepatic failure was induced in 18 male, Sprague–Dawley rats by i.p. administration of 3 g/kg D-galactosamine, and after 30 min, a group of animals received a subcutaneous injection of 25 mg/kg (DHEA) repeated twice a day (3 days). Exploratory behavior and general activity were evaluated 24 h and 48 h after the treatments by the open field test. Then, brain cortex and cerebellum were used for immunoblotting analysis of PKCε level.Results: DHEA administration showed a significant improvement of locomotor activity both 24 and 48 h after D-galactosamine treatment (****p &lt; 0.0001) but did not ameliorate liver parenchymal degeneration. Western blot analysis revealed a reduced immunoreactivity of PKCε (*p &lt; 0.05) following D-galactosamine treatment in rat cortex and cerebellum. After the addition of DHEA, PKCε increased in the cortex in comparison with the D-galactosamine-treated (***p &lt; 0.001) and control group (*p &lt; 0.05), but decreased in the cerebellum (*p &lt; 0.05) with respect to the control group. PKCε decreased after treatment with NH4Cl alone and in combination with DHEA in both cerebellum and cortex (****p &lt; 0.0001). MTS assay demonstrated the synergistic neurotoxic action of NH4Cl and glutamate pretreatment in cerebellum and cortex along with an increased cell survival after DHEA pretreatment, which was significant only in the cerebellum (*p &lt; 0.05).Conclusion: An association between the DHEA-mediated increase of PKCε expression and the improvement of comatose symptoms was observed. PKCε activation and expression in the brain could inhibit GABA-ergic tone counteracting HE symptoms. In addition, DHEA seemed to ameliorate the symptoms of HE and to increase the expression of PKCε in cortex and cerebellum.

scholarly journals ANTI-INFLAMMATORY EFFECT OF ZINGIBER OFFICINALE ON SPRAGUE DAWLEY RATS

2017 ◽  
Vol 10 (3) ◽  
pp. 353 ◽  
Author(s):  
Rohini Karunakaran ◽  
Ndanusa Abdullahi Hassan ◽  
Uma Sankar A ◽  
Khin Mar Aye
Keyword(s):  
Aqueous Extract ◽  
Diclofenac Sodium ◽  
Zingiber Officinale ◽  
Control Group ◽  
Sprague Dawley ◽  
Reference Drug ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Anti Inflammatory ◽  
Inflammatory Effect

ABSTRACTObjective: To investigate the anti-inflammatory effect of aqueous extract of Zingiber officinale on carrageenan-induced inflammation on SpragueDawley (SD) rats.Methods: SD rats were divided into six of five groups and allowed to acclimatize for 1 week. Inflammation was induced on the animal by injecting theright hand paw with carrageenan (0.1 ml of 1%). Group 1 was given normal saline and served as a control. Group 2 was fed with food and water andserved as the carrageenan control. Group 3 was given 200 mg/kg aqueous extract of ginger, Group 4 with 400 mg/kg aqueous extract of ginger, andGroup 5 with 150 mg/kg diclofenac sodium (reference drug for inflammation).Results: The paw edema in carrageenan-induced SD rats was considerably reduced by treating with 400 mg/kg aqueous ginger extracts whencompared to the untreated SD rats (p<0.001).Conclusion: This study indicates that aqueous extract of Z. officinale possesses anti-inflammatory properties.Keywords: Anti-inflammatory, Sprague Dawley rats, Zingiber officinale, Carrageenan, Edema

Cell Activation and Function Alteration in Rat Livers Induced by Repeated Aluminum Oxide Nanoparticles Exposure

2012 ◽  
Vol 486 ◽  
pp. 75-79
Author(s):  
Xiao Bo Li ◽  
Hao Zheng ◽  
Ran Liu ◽  
Ge Yu Liang
Keyword(s):  
Aluminum Oxide ◽  
Cell Activation ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Sprague Dawley ◽  
Aluminum Oxide Nanoparticles ◽  
Liver Macrophages ◽  
Sprague Dawley Rats ◽  
And Function ◽  
And Control

Liver is a major target of nanoparticles accumulation. Here we have analyzed the liver function and activation of liver macrophages, which are sensitive to alteration of liver internal environment after repeated exposure to aluminum oxide nanoparticles. Sprague-Dawley rats where intraperitoneally injected very two days for 60 dyas with aluminum oxide nanoparticles (50mg/kg), non-nanoaluminum oxide (50mg/kg) and saline. After 60 days exposure, the concentrations of alanine aminotransferase and aspartic transaminase in plasma were significantly higher in nanoaluminum oxide group than non-nanoaluminum oxide and control groups. The number of ED-1+and GFAP+cells in liver of nanoaluminum oxide and non-nanoaluminum oxide groups increased significantly than control group. Compared with non-nanoaluminum oxide, aluminum oxide nanoparticles display potential adverse effects on the hypatocytes and biliary tract of rat liver and less stimulus to macrophages in liver than non-nanoaluminum oxide. It is suggested that part aluminum oxide nanoparticles avoided from phagocytosis by liver macrophages. The effects of aluminum oxide nanoparticles exposure should be assessed for its potential hepatic toxicology.

scholarly journals Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2409 ◽  
Author(s):  
Tubagus Bahtiar Rusbana ◽  
Afifah Zahra Agista ◽  
Wahyu Dwi Saputra ◽  
Yusuke Ohsaki ◽  
Kouichi Watanabe ◽  
...  
Keyword(s):  
Muscle Atrophy ◽  
Rice Bran ◽  
Rat Model ◽  
Muscle Size ◽  
Diabetic Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
And Control ◽  
Diabetic Rat Model

Fermented rice bran (FRB), a prospective supplement, has been proven to ameliorate certain medical conditions. However, its nutraceutical effect on muscle atrophy has never been investigated. The present study aimed to evaluate the effect of FRB on muscle atrophy in a streptozotocin (STZ)-induced diabetic rat model. Three groups of Sprague-Dawley rats, namely the control, STZ, and FRB groups, were treated as follows. The diabetic groups (STZ and FRB) were injected intraperitoneally with STZ (40 mg/kg BW), whereas the control group was injected with the vehicle. The STZ and control groups were fed the AIN93M diet, and the FRB group was fed 10% of FRB based on the AIN93M diet. The diabetic groups had reduced muscle size compared to the control group; however, these changes were alleviated in the FRB group. Moreover, the FRB group had a significantly lower expression of FBXO32/Atrogin-1 and TRIM63/MuRF1 (p < 0.05) due to blocked NF-κB activation. In conclusion, the anti-inflammatory effect of FRB may be beneficial for ameliorating muscle atrophy in diabetic conditions.

COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY

2020 ◽  
Vol 4 (13-16) ◽  
Author(s):  
Daniel Francisco Arencibia Arrebola ◽  
Luis Alfredo Rosario Fernández ◽  
Yolanda Emilia Suárez Fernández ◽  
Alexis Vidal Novoa
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Species Group ◽  
Negative Control ◽  
Control Group ◽  
Alkaline Comet Assay ◽  
Sprague Dawley ◽  
Sd Rats ◽  
Sprague Dawley Rats

<p>This article had as objective to carry out a comparison between Balb/c mice and Sprague Dawley rats as biomodel in the alkaline comet assay, keeping in mind the basal frequency and that induced with Cyclophosphamide, the induction of single strand breaks (SSB) or alkali-labile sites formation on DNA of peripheral blood leukocytes. Ten animal/sex/species/group of Balb/c mice and Sprague Dawley rats were used, and treated for 14 days. Using a negative control group (not treated), two substance-vehicle controls and a positive control received cyclophosphamide 50 mg/kg, via intraperitoneal. After two weeks the alkaline electrophoresis gel was performed for individual cells from leukocytes of peripheral blood to screen for possible DNA damage. The best biomodel in both sexes was the SD rats differing significantly with the results obtained in Balb/c mice keeping in mind the spontaneous and induced values of DNA damage. This study shows SD rats are a more efficient model for preclinical genotoxic evaluation of drugs, vaccines and natural products.</p>

scholarly journals Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1

2012 ◽  
Vol 49 (No. 7) ◽  
pp. 253-258
Author(s):  
Rahman MS
Keyword(s):  
Polymerase Chain Reaction ◽  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Control Group ◽  
Sprague Dawley ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Polymerase Chain

In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously at the dose of 1.0 &times; 10<sup>9</sup> colony forming units (cfu) at different stages of gestation period. The maximum rectal temperature was recorded as 38&deg;C in the infected group within 3 days, whereas in the control group the temperature remained normal (36&deg;C). There were no stillbirths, abortions or premature birth and relapsing fever in the infected SD rats. The pathological findings of infected SD rats were splenomegaly, metritis, swelling of lymph nodes, placentitis associated with lymphocytic and macrophage infiltration. Four hundred ninety-eight base pair DNA was detected in infected tissues through AMOS (Brucella abortus, Brucella melitensis, Brucella ovis, Brucella suis) PCR assay. The AMOS PCR assay was shown to be a valuable tool for&nbsp;diagnosis of infected pregnant Sprague-Dawley rats with B.&nbsp;abortus biotype 1.

scholarly journals Effect of Different Doses of Nitrate Supplements on hepatocellular Damage Markers in Male Sprague Dawley Rats Following One Session of Exercise

2020 ◽  
pp. 119-133
Keyword(s):  
Body Weight ◽  
Low Dose ◽  
Male Rats ◽  
Control Group ◽  
High Dose ◽  
Sprague Dawley ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
And Control ◽  
Different Doses

Background: Hepatocellular damage caused by physical activity or the use of supplements is one of the serious problems facing athletes in various fields. This study aimed to evaluate the effect of different doses of nitric oxide supplements on AST and ALT liver enzymes and the ratio of AST to ALT following a session of eccentric exercise in Sprague Dawley male rats. Materials and Methods: In this study, 36 Sprague Dawley male rats (two months old) were divided into three groups of control, low dose (4.8 mg/kg body weight), and high dose of NO supplements (15.4 mg/kg body weight). Supplements were given to rats for seven days. Subsequently, all three groups of rats were forced to run on a treadmill for 45 min with a speed of 20 m/min, and a slope of -15 degrees. Blood samples were taken directly from cardiac puncture of rats 24 h after the running exercise. Blood serum variables of the study were measured afterward. Results: Low dose of nitrate supplements did not change AST and ALT indices, while the high dose of nitrate supplements increased ALT serum level and decreased AST to ALT ratio, compared to a low dose of NO supplements and control group. Conclusion: Based on the obtained results, the consumption of a low dose of NO supplements does not change hepatocellular damage markers, while the high dose of NO supplements causes degeneration of hepatic cells in athletes.

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