scholarly journals In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

2008 ◽  
Vol 19 (4) ◽  
pp. 301-305 ◽  
Author(s):  
Giovanna Pires da Silva Ribeiro de Rezende ◽  
Luciane Ribeiro de Rezende Sucasas da Costa ◽  
Fabiana Cristina Pimenta ◽  
Daniela Abrão Baroni
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Calcium Hydroxide ◽  
Propylene Glycol ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Control Group ◽  
Propolis Extract ◽  
Inhibition Zones

The purpose of this study was to evaluate the antimicrobial activity of two experimental pastes containing propolis extract associated with calcium hydroxide against polymicrobial cultures collected from 16 necrotic and fistulae root canals in primary molars of 4-8-year-old children of both sexes. The agar-well diffusion technique was used to determine the antimicrobial activity of the following pastes: 11.0% ethanolic extract of propolis (EEP) + calcium hydroxide; and 11.0% extract of propolis without ethanol (EP) + calcium hydroxide. EEP, EP and the association of calcium hydroxide and propylene glycol (CHP) was used as the positive control groups, and propylene glycol was used as a negative control group. Friedman and Wilcoxon tests were used to compare the data from the microbial growth inhibition zones (p<0.05). Paste 1 and Paste 2 showed larger growth inhibition zones against microorganisms from root canal samples than CHP (p=0.021 and 0.003, respectively). Paste 2 tended to have larger growth inhibition zones than Paste 1 (p=0.053). The association between propolis and calcium hydroxide was effective in controlling dental infections in vitro.

scholarly journals In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms

2002 ◽  
Vol 13 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Caio Cezar Randi Ferraz ◽  
Morgana Eli Vianna ◽  
Pedro Luiz Rosalen ◽  
Alexandre Augusto Zaia ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Stainless Steel ◽  
Growth Inhibition ◽  
Calcium Hydroxide ◽  
Agar Medium ◽  
Diffusion Method ◽  
Root Canals ◽  
Zones Of Inhibition ◽  
Agar Diffusion Method

The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide in combination with several vehicles against some microorganisms commonly isolated from root canals. Antimicrobial activity was determined by the agar diffusion method. Stainless-steel cylinders were placed on each inoculated agar medium. The test medicaments and their controls were placed inside the cylinders. The zones of growth inhibition were measured and recorded after incubation for each plate and the results were analyzed statistically (ANOVA). The in vitro antimicrobial effects of the medications were ranked from strongest to weakest as follows: Ca(OH)2 + CMCP + glycerine, Ca(OH)2 + CMCP, Ca(OH)2 + glycerine, Ca(OH)2 + anesthetic, Ca(OH)2 + saline, Ca(OH)2 + H2O, Ca(OH)2 + polyethyleneglycol. The pastes with oily vehicles showed significantly larger mean zones of inhibition compared to those with aqueous or viscous vehicles. It was concluded that diffusion and antimicrobial activity of calcium hydroxide were affected by the type of vehicle used.

scholarly journals ANÁLISE ANTIMICROBIANA DO USO DA NANOPARTÍCULA DE PRATA COM HIDRÓXIDO DE CÁLCIO COMO MEDICAÇÃO INTRACANAL

2021 ◽  
Vol 4 (7) ◽  
pp. 1-9
Author(s):  
Julia de Oliveira NEVES ◽  
Franciele de Oliveira DIAS ◽  
Laís Stabile PRONI ◽  
Maria Letícia Oliveira e FREITAS ◽  
Carolina Simonetti LODI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Calcium Hydroxide ◽  
Culture Medium ◽  
Negative Control ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Ag Nps ◽  
Heating Chamber ◽  
Colony Forming Units

O objetivo desse trabalho foi avaliar a atividade antimicrobiana do hidróxido de cálcio (HC) associada a nanopartículas de prata (Ag-NPs) sobre biofilme de Enterococus faecalis. 144 espécimes de dentina foram inoculadas em placas contendo meio de cultura com E. faecalis (ATCC 29212) para a formação de biofilme.  As medicações foram preparadas na proporção de 1:1 de HC e soro estéril e, em seguida, pesada e adicionada às concentrações de Ag-NPs 2,5%, 5% e 10%. Decorridos 14 dias, os espécimes foram lavados, transferidos para uma nova placa onde as medicações foram colocadas sobre o biofilme e deixados em estufa a 37°C por 2, 7 e 14 dias. Espécimes sem tratamento foram usados como controle negativo. Após cada tempo experimental, os espécimes foram lavados, agitados, diluídos e plaqueados em triplicata em M-Enterococcus. As unidades formadoras de colônia foram contadas e analisadas estatisticamente (p<0,05). Os resultados mostraram que não houve diferença estatística entre a medicação de HC e o grupo controle nos períodos de 2 e 7 dias, mesmo quando associados às diferentes concentrações da Ag-NPs. Após 14 dias de contato direto com a medicação, houve uma redução do biofilme bacteriano nos grupos de HC sozinho e associado à prata, quando comparado com o grupo controle, mas nenhuma diferença foi observada entre os grupos de HC. Diante dos resultados, podemos concluir que a associação da Ag-NPs não contribuiu para a atividade antimicrobiana do HC.   ANTIMICROBIAL ANALYSIS OF THE USE OF SILVER NANOPARTICLES WITH CALCIUM HYDROXIDE AS INTRACANAL MEDICATION   ABSTRACT This paper aims at evaluating the antimicrobial activity of calcium hydroxide (CH) in association with silver nanoparticles (AgNPs) over the Enterococus faecalis biofilm.144 dentine specimens were inoculated in plates containing culture medium with E. faecalis (ATCC 29212) to biofilm formation. Medication was prepared in proportion 1:1 of CH and sterile serum, then weighted and added to 2,5%, 5%, and 10% AgNPs concentrations. No earlier than 14 days, the specimens were washed, transferred to a new plate where medication was applied over the biofilm and rested in heating chamber at 37 ºC during 2, 7, and 14 days. Specimens with no treatment were used as a negative control. After each trial time, the specimens were washed, mixed, diluted, and plated in triplicate at M-Enterococcus. The colony-forming units were counted and statistically analyzed (p<0,05). The results showed that there was no statistical difference between CH medication and the group of control during the period of 2 and 7 days, even when they were associated with different concentrations of AgNPs. After 14 days of direct contact with the medication, there was a reduction of the bacterial biofilm in the groups of CH itself and associated with silver, when compared to the control group, but no difference was observed in the group of CH. In view of the results, we can conclude that the association of AgNPs does not contribute to the antimicrobial activity of the CH.   Keywords: Antibacterial.  Silver. Endodontics.  Enterococcus faecalis.

scholarly journals Antimicrobial activity of calcium hydroxide associated with a new vehicle (Triethanolamine)

2016 ◽  
Vol 19 (1) ◽  
pp. 43
Author(s):  
Jaci Simi Junior ◽  
Ricardo Machado ◽  
Rafael Stiz ◽  
Cristiane Figueiredo ◽  
Luiz Pascoal Vansan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Olive Oil ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Culture Medium ◽  
Significance Level ◽  
Petri Dishes ◽  
Antimicrobial Effectiveness

<p><strong>Objective:</strong> The aim of this study was to evaluate the antimicrobial effectiveness of calcium hydroxide associated with a two conventional (anesthetic liquid and olive oil) and a new vehicle (Triethanolamine). <strong>Material and methods:</strong> Initially, microorganisms were collected from four upper incisors presenting unsatisfactory root canal treatments and persistent apical periodontitis from the same patient using sterile absorbent paper cones placed inside the root canal following initial access and root filling removal. Next, they were grown in a BHI culture medium for 24 h. Afterwards, they were placed in 10 Petri dishes with 3 holes, where different formulations of the medications studied were placed. After 48 and 72 h, readings were taken of the growth inhibition halos. <strong>Results:</strong> After using Kruskal-Wallis test with significance level of 1% (α = 0.01) it was observed that, calcium hydroxide associated with Triethanolamine, produced results similar to those produced by the anesthetic liquid. Both combinations were superior to the association with olive oil. <strong>Conclusion:</strong> Considering the results of this <em>in vitro</em> study, it is possible to consider that Triethanolamine can be a viable alternative to be used as a vehicle associated with the calcium hydroxide.</p><p><strong>Keywords:</strong> Vehicles; Calcium hydroxide; Endodontics.</p>

scholarly journals Comparative Antimicrobiotic Efficacy Test of Triple Antibiotic Paste, Double Antibiotic Paste with Fungicide and Calcium Hydroxide with Chitosan as Vehicle against Enterococcus faecalis: An In vitro Study

2021 ◽  
pp. 13-22
Author(s):  
Sudhakar Naidu ◽  
Mahendravarma Nadimpalli ◽  
Gowtam Dev Dondapati ◽  
Thangi Sowjanya ◽  
Srivalli Podili ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Diffusion Test ◽  
Triple Antibiotic Paste ◽  
Antibiotic Powder ◽  
Inhibition Zones ◽  
Agar Well Diffusion Assay

Introduction: This present research aimed to assess the in vitro antimicrobial efficacy of triple antibiotic paste and calcium hydroxide with two different vehicles against Enterococcus faecalis (E. faecalis). Materials and Methodology: An agar well diffusion assay was used to determine the experimental medicaments' efficacy against E. faecalis. Medicaments were divided into six groups, which includes Triple antibiotic powder (TAP) with saline or chitosan, Double antibiotic powder with fungicide (DAP 1) with saline or Chitosan, and calcium hydroxide with saline or Chitosan. These medicines were tested in an agar well diffusion test for three days, i.e., 1,4,7 days. The diameters of growth inhibition zones were recorded and compared for each group were tested in an agar well diffusion test for three days, i.e., 1,4,7 days. The diameters of growth inhibition zones were recorded and compared for each group. These medicaments were evaluated for three days an agar well diffusion test, i.e., on 1,4,7 days. The inhibition zones diameters for each group were recorded and compared — the differences between groups analyzed by Kruskal-Wallis and Mann-Whitney U tests. Results: The largest inhibition zones were observed for the triple antibiotic powder with chitosan and the smallest for Ca(OH)2 with saline. As days progress, they produced lesser antibacterial effects in all groups. Conclusion: Triple antibiotic paste and Chitosan were more effective in eliminating microorganisms than calcium hydroxide and DAP 1.

scholarly journals Diffusion of calcium hydroxide through dentinal tubules of retreated root canals: An in vitro study

2018 ◽  
Vol 12 (03) ◽  
pp. 386-392 ◽  
Author(s):  
Mohammed Mustafa ◽  
Wafa Hassan Alaajam ◽  
Ahmed Abdul Azeim ◽  
Najla Aedh Alfayi ◽  
Rahaif Misfer Alqobty ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Normal Saline ◽  
Root Canal ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Group I ◽  
Group Ii

ABSTRACTObjective: Studies about the dispersion of hydroxyl ions through dentin of endodontically retreated teeth are scarce. The present study aims at examining the diffusion of calcium hydroxide in vitro by recording the pH changes of retreated root canal using two types of calcium hydroxide preparations. Materials and Methods: A total of 45 recently extracted single-rooted mandibular first premolars were collected and cleaned from calculus and remains of periodontal tissue. The teeth so collected were stored in thymol 10% solution till use. During collection, teeth with cracks, open apices, and restorations were excluded. Root canal preparations were performed using Protaper rotary system files, till #F3 file. All canals were obturated using cold lateral condensation and zinc oxide-based sealer. After 7 days, all gutta-percha were removed and the canals were irrigated with normal saline. The teeth were then randomly distributed into three groups. Canals in Group I were kept empty without any dressing to serve as a negative control group, Canals in Group II were filled with a freshly prepared mixture of calcium hydroxide powder with normal saline solution, and Group III was filled with Metapex. Periodically, the calcium hydroxide material was removed and the pH was measured using pH meter at 7, 10, 14, and 30 days. Results: The pH values of Group II and III ranged between 9.2 and 11.2. This was found to be highly significant against the negative control group. After 7 and 10 days, freshly mixed Ca(OH)2 showed the higher pH than the creamy Metapex, and statistically the difference was highly significant after 10 days (P<0.001). After 14 days, Metapex group pH was higher than freshly mixed Ca(OH)2, but it was not statistically significant as they reached nearly the same pH after 30 days (P>0.05). Conclusion: All Ca(OH)2 preparations had high pH around the roots after 7 and 10 days. Metapex continued to have higher pH after 14 days.

scholarly journals The Effect of Mirabilis jalapa Leaf Ethanolic Extract against Streptococcus pyogenes

1970 ◽  
Vol 2 (1) ◽  
pp. 26
Author(s):  
Bagus Satrio Pambudi ◽  
Enny Suswati ◽  
Jauhar Firdaus
Keyword(s):  
Streptococcus Pyogenes ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
In Vitro Techniques ◽  
Mirabilis Jalapa

Streptococcus pyogenes is a Gram positive bacteria that commonly cause disease in human. If not treated immediately, this bacteria can cause serious complication such as reumatic fever that causing heart valve tissue damage. Penicilin, drug of choice to eradicate S. pyogenes, oftenly cause various side effects such as anaphylaxis. This study aimed to investigate the effect of Mirabilis jalapa leaf ethanolic extract against S. pyogenes growth using in vitro techniques. The study design was a quasi experimental design. S. pyogenes culture as the study subject were divided into positive control group (penicilin V 100 IU), negative control group (NaCMC 0,5%), and eight treatment groups that were given with M. jalapa leaf ethanolic extract as much as 0,1 mg/ml, 1 mg/ml, 5 mg/ml, 10 mg/ml, 20 mg/ml, 30 mg/ml, 40mg/ml, and 50 mg/ml. After 24 hours incubation periods, the inhibition zone were found in all treatment group except in concentration 0,1 mg/ml. This study showed that M. jalapa leaf ethanolic extract could inhibit the growth of S. pyogenes. There was significant correlation between the concentration of M. jalapa leaf ethanolic extract and the diameter of inhibition zone (p=0,00), the higher concentration of M. jalapa leaf extract, the larger diameter of inhibition zone of S. pyogenes. Keywords: Mirabilis jalapa, leaf extract, antimicrobial activity

scholarly journals Antimicrobial activity and biocompatibility of a calcium hydroxide - and aloe vera-based intracanal medication

2017 ◽  
Vol 74 (3) ◽  
pp. 180
Author(s):  
Liza Porcaro Bretas ◽  
Carolina Oliveira de Lima ◽  
Nadia Rezende Barbosa Raposo ◽  
Beatriz Julião Vieira Aarestrup ◽  
Maíra Do Prado ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Propylene Glycol ◽  
Subcutaneous Tissue ◽  
Aloe Vera ◽  
Control Group ◽  
Group Iii ◽  
The Third ◽  
Histological Characteristics ◽  
Group Ii

Objective: to investigate the antimicrobial activity and biocompatibility of the calcium hydroxide [Ca(OH)2] + propylene glycol (PEG) + aloe vera (AV) paste in comparison with other pastes used as intracanal medication. Material and Methods: there was evaluated 3 intracanal medication based on calcium hydroxide and propylene glycol, varying only the third component. In group 1, the third component was camphorated paramonoclorofenol (PMCC); in group II, chlorhexidine (CLX); and in group III, aloe vera. The antimicrobial activity was analyzed through the direct contact of the intracanal medication pastes with strains of Enterococcus faecalis; Kocuria rhizophila; Pseudomonas aeruginosa and Candida albicans. The biocompatibility was evaluated in subcutaneous tissue of rats during experimental 7, 21 and 63 days. Results: it was observed that the Group II showed the best results regarding antimicrobial activity, followed by group III and I. The Ca(OH)2 + PEG + AV paste was considered biocompatible since it presented discrete fibrosis and suggestive histological characteristics of normal healing after 63 days, approaching the control group. Conclusion: the association of Ca (OH)2 + PEG + AV showed antibacterial activity and adequate biocompatibility when compared with commonly pastes used as intracanal medication.

scholarly journals THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO

2018 ◽  
Vol 12 (4) ◽  
Author(s):  
Nuzul Asmilia ◽  
T Armansyah TR ◽  
Dwinna Aliza ◽  
Juli Melia ◽  
Erdiansyah Rahmi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Antiplasmodial Activity ◽  
Control Group ◽  
Ic50 Value

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.

scholarly journals PERBEDAAN ANGIOGENESIS PADA PULPA SETELAH APLIKASI EKSTRAK PROPOLIS DAN KALSIUM HIDROKSIDA

2019 ◽  
Vol 9 (1) ◽  
pp. 48
Author(s):  
Camelia Ariesdyanata ◽  
Cecilia G.J. Lunardhi ◽  
Agus Subiwahjudi
Keyword(s):  
Blood Vessels ◽  
Calcium Hydroxide ◽  
Histological Examination ◽  
Glass Ionomer Cement ◽  
Negative Control ◽  
Control Group ◽  
Propolis Extract ◽  
Pulp Capping ◽  
Significant Difference ◽  
Pulp Exposure

Background : Propolis is a resinous hive product collected by bees ( Apis Mellifera ) from tree buds and mixed with secreted bee wax in order to avoid bacterial contamination in the hive, and also to seal it. Propolis is employed for the treatment of various infectious diseases because it is wellknown that is has antibacterial and anti-inflammatory properties. Calcium hydroxide was introduced to the dental profession in 1921 and has been considered the “gold standard” for direct pulp capping materials   in the past decades. Aims :This research is to investigate the development of new blood vessels ( angiogenesis ) in rat's dental pulp following application of propolis extract and calcium hydroxide. Methods : There was 43 Strain Wistar rats  of 8–16 week old and 200–250 grams in weight were used in this study. The rats were randomly divided into 6 groups. Pulp exposures were performed on the occlusal surface of right maxillary first molars. At the 1st and 4th groups, as the control group, without pulp capping paste. At the 2rd and 5th groups, pulp exposure was applied with propolis extract. And at 3th and 6th groups pulp exposure was applied with calcium hydroxide ,and the 7th group is negative control is a normal teeth. Pulp exposure was applied with propolis extract. After that, all of the cavities were filled with light cured glass ionomer cement as a permanent filling. Animals on the 1st, 2rd, and 3th groups were decaputed after 7x24 hour  post  pulp capping material application and were sent for histological examination which new blood vessels ( angiogenesis )  cells were present. And at the 4th, 5th, 6th groups were culled after 7x 24 hour post  pulp capping material application and were sent for histological examination which new blood vessels ( angiogenesis ) evaluated were present. Result : All sample were histopathological examinated and data was statiscally analysed using one way ANOVA the histological analysis revealed that the development of the new blood vessels  occurred in all group. The new blood vessels ( angiogenesis ) of propolis extract group was milder compared to the control and calcium hydroxide group, with statistical analysis showed significant difference (p > 0.05). Conclusion: The development of new blood vessels is earlier happened in group capping material containing propolis and which show with reduce the amount of the new blood vessels in days 7 and 14 than the other group.

scholarly journals Lemongrass (Cymbopogon Citratus) Essential Oil Inhibits Candida Albicans Growth in Vitro

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 142-147
Author(s):  
Yoga Mulia Pratama ◽  
Bryan Pandu Permana
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Morbidity ◽  
Cymbopogon Citratus ◽  
Group I ◽  
Inhibition Zones

Candidiasis is an important nosocomial infection with high morbidity value, high mortality rate, and expensive clinical cost. However, public access to well-developed treatment is not acquired. Current medicines mostly used like -azol drugs had been showing the resistant effects because of the long period use of the same medicines. Alternative medicines like herb medicine are discussed to reduce multi-resist infections, such as lemongrass Cymbopogon citratus essential oil. This study aims to know the effect of lemongrass essential oil on the growth of Candida albicans in vitro. Laboratory experimental (in vitro) was conducted in this research. Candida albicans strains were being used as the objects, which were picked by random sampling. Candida albicans were divided into 12 groups of treatment, the group I was treated with ethanol 96% as the negative control, group II with fluconazole 25μg as the positive control, and group III-XII with Cymbopogon citratus essential oil with 10%, 20% to 100% concentrated. The diameter of inhibition zones was measured after 2x24 hours incubation. The data was analyzed by post-hoc Mann Whitney test with SPSS 18.0 (p<0.05 considered as significant). Cymbopogon citratus essential oil showed antifungal activity to the Candida albicans began in the 10% to 100% concentration (p<0.05). Inhibition zones with the 50% to 100% concentration had similar results to the positive control (p>0.05). The Cymbopogon citratus essential oil has an antifungi effect toward Candida albicans in vitro significantly by the negative control.

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