scholarly journals ANÁLISE ANTIMICROBIANA DO USO DA NANOPARTÍCULA DE PRATA COM HIDRÓXIDO DE CÁLCIO COMO MEDICAÇÃO INTRACANAL

2021 ◽  
Vol 4 (7) ◽  
pp. 1-9
Author(s):  
Julia de Oliveira NEVES ◽  
Franciele de Oliveira DIAS ◽  
Laís Stabile PRONI ◽  
Maria Letícia Oliveira e FREITAS ◽  
Carolina Simonetti LODI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Calcium Hydroxide ◽  
Culture Medium ◽  
Negative Control ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Ag Nps ◽  
Heating Chamber ◽  
Colony Forming Units

O objetivo desse trabalho foi avaliar a atividade antimicrobiana do hidróxido de cálcio (HC) associada a nanopartículas de prata (Ag-NPs) sobre biofilme de Enterococus faecalis. 144 espécimes de dentina foram inoculadas em placas contendo meio de cultura com E. faecalis (ATCC 29212) para a formação de biofilme.  As medicações foram preparadas na proporção de 1:1 de HC e soro estéril e, em seguida, pesada e adicionada às concentrações de Ag-NPs 2,5%, 5% e 10%. Decorridos 14 dias, os espécimes foram lavados, transferidos para uma nova placa onde as medicações foram colocadas sobre o biofilme e deixados em estufa a 37°C por 2, 7 e 14 dias. Espécimes sem tratamento foram usados como controle negativo. Após cada tempo experimental, os espécimes foram lavados, agitados, diluídos e plaqueados em triplicata em M-Enterococcus. As unidades formadoras de colônia foram contadas e analisadas estatisticamente (p<0,05). Os resultados mostraram que não houve diferença estatística entre a medicação de HC e o grupo controle nos períodos de 2 e 7 dias, mesmo quando associados às diferentes concentrações da Ag-NPs. Após 14 dias de contato direto com a medicação, houve uma redução do biofilme bacteriano nos grupos de HC sozinho e associado à prata, quando comparado com o grupo controle, mas nenhuma diferença foi observada entre os grupos de HC. Diante dos resultados, podemos concluir que a associação da Ag-NPs não contribuiu para a atividade antimicrobiana do HC.   ANTIMICROBIAL ANALYSIS OF THE USE OF SILVER NANOPARTICLES WITH CALCIUM HYDROXIDE AS INTRACANAL MEDICATION   ABSTRACT This paper aims at evaluating the antimicrobial activity of calcium hydroxide (CH) in association with silver nanoparticles (AgNPs) over the Enterococus faecalis biofilm.144 dentine specimens were inoculated in plates containing culture medium with E. faecalis (ATCC 29212) to biofilm formation. Medication was prepared in proportion 1:1 of CH and sterile serum, then weighted and added to 2,5%, 5%, and 10% AgNPs concentrations. No earlier than 14 days, the specimens were washed, transferred to a new plate where medication was applied over the biofilm and rested in heating chamber at 37 ºC during 2, 7, and 14 days. Specimens with no treatment were used as a negative control. After each trial time, the specimens were washed, mixed, diluted, and plated in triplicate at M-Enterococcus. The colony-forming units were counted and statistically analyzed (p<0,05). The results showed that there was no statistical difference between CH medication and the group of control during the period of 2 and 7 days, even when they were associated with different concentrations of AgNPs. After 14 days of direct contact with the medication, there was a reduction of the bacterial biofilm in the groups of CH itself and associated with silver, when compared to the control group, but no difference was observed in the group of CH. In view of the results, we can conclude that the association of AgNPs does not contribute to the antimicrobial activity of the CH.   Keywords: Antibacterial.  Silver. Endodontics.  Enterococcus faecalis.

scholarly journals Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

2013 ◽  
Vol 24 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Roberta Vieira Farac ◽  
Antonio Carlos Pizzolitto ◽  
Juliane Maria Guerreiro Tanomaru ◽  
Renata Dornelles Morgental ◽  
Regina Karla de Pontes Lima ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Ex Vivo ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Human Teeth ◽  
Root Canals ◽  
Colony Forming Units

This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.

scholarly journals In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

2008 ◽  
Vol 19 (4) ◽  
pp. 301-305 ◽  
Author(s):  
Giovanna Pires da Silva Ribeiro de Rezende ◽  
Luciane Ribeiro de Rezende Sucasas da Costa ◽  
Fabiana Cristina Pimenta ◽  
Daniela Abrão Baroni
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Calcium Hydroxide ◽  
Propylene Glycol ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Control Group ◽  
Propolis Extract ◽  
Inhibition Zones

The purpose of this study was to evaluate the antimicrobial activity of two experimental pastes containing propolis extract associated with calcium hydroxide against polymicrobial cultures collected from 16 necrotic and fistulae root canals in primary molars of 4-8-year-old children of both sexes. The agar-well diffusion technique was used to determine the antimicrobial activity of the following pastes: 11.0% ethanolic extract of propolis (EEP) + calcium hydroxide; and 11.0% extract of propolis without ethanol (EP) + calcium hydroxide. EEP, EP and the association of calcium hydroxide and propylene glycol (CHP) was used as the positive control groups, and propylene glycol was used as a negative control group. Friedman and Wilcoxon tests were used to compare the data from the microbial growth inhibition zones (p<0.05). Paste 1 and Paste 2 showed larger growth inhibition zones against microorganisms from root canal samples than CHP (p=0.021 and 0.003, respectively). Paste 2 tended to have larger growth inhibition zones than Paste 1 (p=0.053). The association between propolis and calcium hydroxide was effective in controlling dental infections in vitro.

In vitro and in vivo Assessment of Silver Nanoparticles Against Clostridium botulinum Type A Botulinum

2019 ◽  
Vol 16 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Mohammad Aminianfar ◽  
Siavash Parvardeh ◽  
Mohsen Soleimani
Keyword(s):  
Silver Nanoparticles ◽  
Botulinum Toxin ◽  
Clostridium Botulinum ◽  
Lethal Dose ◽  
Type A ◽  
Positive Control ◽  
Negative Control ◽  
Laboratory Animals ◽  
Control Group ◽  
Nano Silver

Background: Clostridium botulinum causes botulism, a serious paralytic illness that results from the ingestion of a botulinum toxin. Because silver nanoparticle products exhibit strong antimicrobial activity, applications for silver nanoparticles in healthcare have expanded. Therefore, the objective of the current study was to assess a therapeutic strategy for the treatment of botulism toxicity using silver nanoparticles. Methods: A preliminary test was conducted using doses that produce illness in laboratory animals to determine the absolute lethal dose (LD100) of botulinum toxin type A (BoNT/A) in mice. Next, the test animals were divided into six groups containing six mice each. Groups I, II and III were the negative control (botulinum toxin only), positive control-1 (nano-silver only) and positive control-2 (no treatment), respectively. The remaining groups were allocated to the toxin that was supplemented with three nano-silver treatments. Results: The mortality rates of mice caused by BoNT/A significantly reduced in the treatment groups with different doses and injection intervals of nano-silver when compared to the negative control group. BoNT/A toxicity induced by intraperitoneal injection of the toxin of Clostridium botulinum causes rapid death while when coupled with nano-osilver results in delayed death in mice. Conclusion: These results, while open to future improvement, represent a preliminary step towards the satisfactory control of BoNT/A with the use of silver nanoparticles for human protection against this bioterrorism threat. Further study in this area can elucidate the underlying mechanism for detoxifying BoNT/A by silver nanoparticles.

Thermo-chemical induced production of silver nanoparticles (Ag-NPs) and their antimicrobial activity towards human pathogens

2017 ◽  
Vol 10 (5) ◽  
pp. 1322
Author(s):  
S. Krishnakumar ◽  
R. Divya ◽  
N.R. Kanchana Devi ◽  
G. Keerthana ◽  
A. Ancy Judi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Human Pathogens ◽  
Ag Nps

scholarly journals Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study

2016 ◽  
Vol 45 (2) ◽  
pp. 90-96 ◽  
Author(s):  
Rosa Virginia Dutra de OLIVEIRA ◽  
Yasmin Etienne ALBUQUERQUE ◽  
Denise Madalena Palomari SPOLIDORIO ◽  
Cristiane Yumi KOGA-ITO ◽  
Elisa Maria Aparecida GIRO ◽  
...  
Keyword(s):  
Pilot Study ◽  
Streptococcus Mutans ◽  
Culture Medium ◽  
Negative Control ◽  
Caries Experience ◽  
Control Group ◽  
Streptococcus Sobrinus ◽  
Significance Level ◽  
Biomass Formation ◽  
Microtiter Plates

Abstract Introduction Frequent consumption of sugars and the presence of Streptococcus mutans and Streptococcus sobrinus are correlated with higher caries experience. Objective The aim of this pilot study was to elucidate the effect of different fermentable carbohydrates on biomass formation and acidogenicity of S. mutans and S. sobrinus biofilms. Material and method Single and dual-species biofilms of S. mutans ATCC 25175 and S. sobrinus ATCC 27607 were grown at the bottom of microtiter plates at equal concentrations for 24 h at 37 °C under micro-aerobic atmosphere. Carbohydrates were added at 2% concentration: maltose, sucrose, glucose and lactose. BHI Broth (0.2% glucose) was used as negative control. Acidogenicity was assessed by measuring the pH of spent culture medium after 24 h, immediately after refreshing the culture medium and for the next 1 h and 2 h. Crystal violet staining was used as an indicator of the total attached biofilm biomass after 24 h incubation. Data were analyzed by two-way ANOVA followed by Bonferroni post hoc test. Significance level was set at 5%. Result All carbohydrates resulted in higher biomass formation in single- and dual-species biofilms when compared to the control group. Sucrose, lactose and maltose showed higher acidogenicity than the control group in both single- and dual-species biofilms after 24 h. Conclusion These findings indicate that the type of biofilm (single- or dual-species) and the carbohydrate used may influence the amount of biomass formed and rate of pH reduction.

scholarly journals Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

2021 ◽  
Vol 9 (A) ◽  
pp. 760-765
Author(s):  
Yos Banne ◽  
Olfie Sahelangi ◽  
Steven Soenjono ◽  
Elisabeth Natalia Barung ◽  
Selfie Ulaen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Distilled Water ◽  
Lc50 Value ◽  
Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

Antimicrobial Activity of PET-Silver Nanocomposite Filaments

2018 ◽  
Vol 930 ◽  
pp. 212-217
Author(s):  
Marcos Antônio Guerra ◽  
Jeferson Prado Swerts ◽  
Mei Abe Funcia ◽  
Maria Gabriela Nogueira Campos
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Dermal Fibroblasts ◽  
Klebsiella Pneumonia ◽  
Dyeing Process ◽  
Colony Forming Units ◽  
Silver Nanocomposite

This study evaluated the antimicrobial activity of PET-Silver nanocomposite filaments at different concentrations (0, 0.180%, 0.135%, 0.090%, 0.045% and 0.022% w/w) of silver nanoparticles in order to determine the minimum inhibitory concentration and minimum bactericidal concentration of silver incorporated in the PET matrix. The in vitro antibacterial activity was evaluated by the AATCC standard 100: 2012 method, against Staphylococcus aureus ATCC 6538, and Klebsiella pneumonia ATCC 4532. The filaments were tested after one and twenty-one months of preparation to evaluate the effect of time on the antimicrobial activity of the nanocomposites. Moreover, the antimicrobial activity was also evaluated after dyeing the filaments. The silver-free PET filaments have not demonstrated antimicrobial activity and cytotoxicity against human dermal fibroblasts. Nevertheless, excepted for the filament with 0.022% of silver nanoparticles, all PET-Silver nanocomposites reduced more than 99% the colony-forming units (CFU) of Staphylococcus aureus and Klebsiella pneumonia after one and twenty-one months of preparation. This suggests that the MIC of silver nanoparticles incorporated in the PET matrix is lower than 220 ppm (w/w) and the MBC is between 0.022 and 0.045% (w/w). However, after the dyeing process, no antimicrobial activity was observed for any PET-Silver nanocomposite filaments. This may be attributed to the release of silver from the PET matrix during the dyeing process or to the reaction/inactivation of the silver ions by the salts used in this chemical treatment.

scholarly journals Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 442 ◽  
Author(s):  
Fabio Fontecha-Umaña ◽  
Abel Guillermo Ríos-Castillo ◽  
Carolina Ripolles-Avila ◽  
José Juan Rodríguez-Jerez
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Bacterial Biofilm ◽  
Antimicrobial Efficacy ◽  
Ag Nps ◽  
Zno Nps ◽  
E Coli ◽  
Food Contact Surfaces

Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions ≥ 2 log CFU/cm2) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm2). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm2) and S. aureus (3.84 log CFU/cm2). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm2) and S. aureus (4.11 log CFU/cm2). The results also showed a high correlation between concentration levels and the bacterial activity of Ag–ZnO-NPs (R2 = 0.97 for S. aureus, and R2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.

scholarly journals Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23)

2010 ◽  
Vol 21 (1) ◽  
pp. 24-31 ◽  
Author(s):  
Fernanda Campos Rosetti Lessa ◽  
Andreza Maria Fábio Aranha ◽  
Josimeri Hebling ◽  
Carlos Alberto de Souza Costa
Keyword(s):  
Culture Medium ◽  
Cell Metabolism ◽  
Mineral Trioxide Aggregate ◽  
Negative Control ◽  
Control Group ◽  
Cytotoxic Effects ◽  
Significant Difference ◽  
Cell Organization ◽  
New Formulation ◽  
And Control

This study evaluated the cytotoxic effects of 2 mineral trioxide aggregate (MTA) cements - White-MTA-Angelus and a new formulation, MTA-Bio - on odontoblast-like cell (MDPC-23) cultures. Twenty-four disc-shaped (2 mm diameter x 2 mm thick) specimens were fabricated from each material and immersed individually in wells containing 1 mL of DMEM culture medium for either 24 h or 7 days to obtain extracts, giving rise to 4 groups of 12 specimens each: G1 - White-MTA/24 h; G2 - White-MTA/7 days; G3 - MTA-Bio/24 h; and G4 - MTA-Bio/7 days. Plain culture medium (DMEM) was used as a negative control (G5). Cells at 30,000 cells/cm² concentration were seeded in the wells of 24-well plates and incubated in a humidified incubator with 5% CO2 and 95% air at 37ºC for 72 h. After this period, the culture medium of each well was replaced by 1 mL of extract (or plain DMEM in the control group) and the cells were incubated for additional 2 h. Cell metabolism was evaluated by the MTT assay and the data were analyzed statistically by ANOVA and Tukey's test (α=0.05). Cell morphology and the surface of representative MTA specimens of each group were examined by scanning electron microscopy. There was no statistically significant difference (p>0.05) between G1 and G2 or between G3 and G4. No significant difference (p>0.05) was found between the experimental and control groups either. Similar cell organization and morphology were observed in all groups, regardless of the storage periods. However, the number of cells observed in the experimental groups decreased compared to the control group. MTA-Bio presented irregular surface with more porosities than White-MTA. In conclusion, White-MTA and MTA-Bio presented low cytotoxic effects on odontoblast-like cell (MDPC-23) cultures.

scholarly journals Effect of Hydrogel Epigallocatechin-3-Gallate (EGCG) to the Number of Fibroblast Cell Proliferation in the Perforation of Wistar Rat Tooth Pulp

2020 ◽  
Vol 9 (2) ◽  
pp. 93
Author(s):  
Ira Widjiastuti ◽  
Setyabudi Setyabudi ◽  
Kun Ismiyatin ◽  
Djoko Agus Purwanto ◽  
Tiara Sukmawati
Keyword(s):  
Cell Proliferation ◽  
Calcium Hydroxide ◽  
Wistar Rats ◽  
Radical Scavenging ◽  
Fibroblast Cell ◽  
Negative Control ◽  
Tooth Pulp ◽  
Control Group ◽  
Current Standard ◽  
Control Group Design

Background: Pulpitis can occur because the deep cavity preparation and it causes increasing of NO levels. Perforated teeth require direct pulp capping (DPC) treatment. The current standard DPC material is calcium hydroxide. However, several studies have found weaknesses of calcium hydroxide that can affect the success of DPC treatment and new, more biocompatible materials are needed. Epigallocatechin-3-gallate (EGCG) in green tea has many benefits, including antioxidant, anticolagenase, anticancer, anti-inflammatory and has the ability of radical scavenging to clean NO so that pulp healing can occur better by increasing the number of fibroblast cells that play a role in wound healing. Purpose: To determine the concentration of hydrogel EGCGs that are effective in increasing the number of fibroblast cell proliferation in the dental pulp perforation of Wistar rats. Method: This research is a laboratory experimental study with a randomized post test only control group design. Samples used in the study were 24 male Wistar rats which were divided into four groups, namely the negative control group and the treatment group were given EGCG 60 ppm, 90 ppm, and 120 ppm and were decapitated on the 7th day after treatment. The maxilla and the 1st molar were taken and decalcified, to process the HPA reading with HE staining. Observations were made using a microscope with a magnification of 400x. Results: There were significant differences in the treatment groups with 60 ppm and 90 ppm hydrogel hydrogels on the results of the Oneway ANOVA difference test (p <0.05). Conclusion: The concentration of hydrogel EGCG which is effective in increasing fibroblast cell proliferation is 90 ppm.

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