scholarly journals The anti-adherence effect of Lippia sidoides Cham: extract against microorganisms of dental biofilm

2013 ◽  
Vol 15 (1) ◽  
pp. 41-46 ◽  
Author(s):  
A.C.L. Albuquerque ◽  
M.S.V. Pereira ◽  
D.F. Silva ◽  
L.F. Pereira ◽  
F.A.C. Viana ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Folk Medicine ◽  
Tooth Surface ◽  
Bacterial Strains ◽  
Lippia Sidoides ◽  
Dental Biofilm ◽  
Scientific Exploration ◽  
Infectious Origin ◽  
In Vitro Simulation

Most illnesses affecting the oral cavity are proven to have infectious origin. Several categories of chemical agents have been used in the chemical control of dental biofilm through strategies that aim at reducing bacterial adhesion and inhibiting the growth and the proliferation of microorganisms on the tooth surface. The use of plants in folk medicine and in Dentistry, as well as the spread of successful cases, has led to scientific exploration, resulting in chemical-pharmacological knowledge of thousands of plants. The present study aimed to evaluate the anti-adherence activity of Lippia sidoides Cham., comparing the results with those of 0.12% chlorhexidine by means of an in vitro simulation of dental biofilm. The studied bacterial strains were Streptococcus mutans, Streptococcus sanguinis and Lactobacillus casei, main responsible for the biofilm adherence. The studied extract was effective in inhibiting the adherence of Streptococcus mutans up to a concentration of 1:16, compared to Chlorhexidine. Lippia sidoides Cham extract showed anti-adherence effect on the major microorganisms responsible for dental biofilm consolidation.

scholarly journals Avaliação in vitro da Atividade Antimicrobiana de Extrato Alcoólico de Própolis Comparado à Solução de Clorexidina 0,12%

2017 ◽  
Vol 19 (2) ◽  
pp. 95
Author(s):  
Maria Paula Jacobucci Botelho ◽  
Amanda Da Silva ◽  
Fernanda Da Conceição Antônio Ferreira ◽  
Lígia Maria Molinari Capel
Keyword(s):  
Drinking Water ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Positive Control ◽  
Negative Control ◽  
Tooth Surface ◽  
Dental Biofilm ◽  
Fermentable Carbohydrates ◽  
Broad Understanding

Apesar dos esforços e do amplo entendimento sobre a cárie dental, está continua bastante prevalente em determinados segmentos da população. A doença apresenta etiologia multifatorial, com a dieta e os micro-organismos envolvidos desenvolvendo papéis fundamentais. Os Streptococcus mutans têm papel fundamental em seu início e é ubíquo em crianças a partir dos sete anos de idade. Para controlar essas bactérias é preciso uma dieta com baixo consumo de sacarose e de carboidratos fermentáveis, o que nem sempre se consegue. Sua remoção da superfície dentária só é possível por meio da fricção mecânica, mas pode-se prevenir sua presença no biofilme dentário através da utilização de antissépticos. O mais utilizado na Odontologia é a clorexidina, porém como não é isenta de efeitos adversos, atualmente, vêm-se estudando produtos alternativos, dentre os quais alguns agentes fitoterápicos. O objetivo deste trabalho foi analisar a atividade da própolis contra os S. mutans em relação à clorexidina. Foi analisada própolis 70% diluída em álcool 96% e após diluída em água potável 5ml, própolis 50%, própolis 12% e clorexidina 0,12% (controle positivo), e solução salina (controle negativo). O operador foi cego em relação às substâncias que estava utilizando. Somente a clorexidina 0.12% e a própolis 12% apresentaram halo de inibição claro. Neste estudo, a clorexidina apresentou melhor atividade contra os S.mutans.Palavras-chave: Clorexidina. Cárie Dentária. Streptococcus mutans.AbstractDespite the efforts and the broad understanding on dental caries, it is still quite prevalent in certain segments of the population. The disease shows a multifactorial etiology, with diet and microorganisms involved developing fundamental roles. The Streptococcus mutans has key role in its beginning and is ubiquitous in children as young as seven years of age. To control these bacteria a diet is necessary with low consumption of sucrose and of fermentable carbohydrates, which is not always possible. The tooth surface removal is only possible through mechanical friction, but it is possible to prevent the presence of dental biofilm through the use of antiseptics. The most widely used in dentistry is chlorhexidine, however as it is not devoid of adverse effects, currently alternative products have been studying, among which some herbal agents. The aim of this study was to analyze the activity of propolis against S. mutans compared to chlorhexidine. Propolis was analyzed diluted in 70% ethanol 96% and further diluted in drinking water 5ml, 50% propolis, propolis 12% and 0.12% chlorhexidine (positive control), and saline (negative control). The operator was blinded for the substances he was using. Only 0.12% chlorhexidine and 12% propolis showed clear inhibition halo. In this study the chlorhexidine presented better activity against S. mutans.Keywords: Chlorhexidine. Dental Caries. Antimicrobials. Streptococcus mutans

scholarly journals Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Fatemeh Ostadhossein ◽  
Parikshit Moitra ◽  
Esra Altun ◽  
Debapriya Dutta ◽  
Dinabandhu Sar ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Ex Vivo ◽  
Bacterial Species ◽  
Reactive Oxygen Species Generation ◽  
Biofilm Inhibition ◽  
16S Rrna Analysis ◽  
Dental Biofilm ◽  
Dental Plaques

AbstractDental plaques are biofilms that cause dental caries by demineralization with acidogenic bacteria. These bacteria reside inside a protective sheath which makes any curative treatment challenging. We propose an antibiotic-free strategy to disrupt the biofilm by engineered clustered carbon dot nanoparticles that function in the acidic environment of the biofilms. In vitro and ex vivo studies on the mature biofilms of Streptococcus mutans revealed >90% biofilm inhibition associated with the contact-mediated interaction of nanoparticles with the bacterial membrane, excessive reactive oxygen species generation, and DNA fragmentation. An in vivo examination showed that these nanoparticles could effectively suppress the growth of S. mutans. Importantly, 16S rRNA analysis of the dental microbiota showed that the diversity and richness of bacterial species did not substantially change with nanoparticle treatment. Overall, this study presents a safe and effective approach to decrease the dental biofilm formation without disrupting the ecological balance of the oral cavity.

scholarly journals Exopolysaccharides Produced by Streptococcus mutans Glucosyltransferases Modulate the Establishment of Microcolonies within Multispecies Biofilms

2010 ◽  
Vol 192 (12) ◽  
pp. 3024-3032 ◽  
Author(s):  
H. Koo ◽  
J. Xiao ◽  
M. I. Klein ◽  
J. G. Jeon
Keyword(s):  
Streptococcus Mutans ◽  
Extracellular Polysaccharide ◽  
Fluorescence Labeling ◽  
Tooth Surface ◽  
Bacterial Cells ◽  
Actinomyces Naeslundii ◽  
Multispecies Biofilm ◽  
Tooth Surfaces ◽  
Glucan Synthesis

ABSTRACT Streptococcus mutans is a key contributor to the formation of the extracellular polysaccharide (EPS) matrix in dental biofilms. The exopolysaccharides, which are mostly glucans synthesized by streptococcal glucosyltransferases (Gtfs), provide binding sites that promote accumulation of microorganisms on the tooth surface and further establishment of pathogenic biofilms. This study explored (i) the role of S. mutans Gtfs in the development of the EPS matrix and microcolonies in biofilms, (ii) the influence of exopolysaccharides on formation of microcolonies, and (iii) establishment of S. mutans in a multispecies biofilm in vitro using a novel fluorescence labeling technique. Our data show that the ability of S. mutans strains defective in the gtfB gene or the gtfB and gtfC genes to form microcolonies on saliva-coated hydroxyapatite surfaces was markedly disrupted. However, deletion of both gtfB (associated with insoluble glucan synthesis) and gtfC (associated with insoluble and soluble glucan synthesis) is required for the maximum reduction in EPS matrix and biofilm formation. S. mutans grown with sucrose in the presence of Streptococcus oralis and Actinomyces naeslundii steadily formed exopolysaccharides, which allowed the initial clustering of bacterial cells and further development into highly structured microcolonies. Concomitantly, S. mutans became the major species in the mature biofilm. Neither the EPS matrix nor microcolonies were formed in the presence of glucose in the multispecies biofilm. Our data show that GtfB and GtfC are essential for establishment of the EPS matrix, but GtfB appears to be responsible for formation of microcolonies by S. mutans; these Gtf-mediated processes may enhance the competitiveness of S. mutans in the multispecies environment in biofilms on tooth surfaces.

scholarly journals THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)

2017 ◽  
Vol 10 (17) ◽  
pp. 30
Author(s):  
Royan Diana ◽  
Hedijanti Joenoes ◽  
Ariadna A Djais
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Single Species ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Vitro Study ◽  
Dental Biofilm ◽  
Significant Difference ◽  
Curcuma Xanthorrhiza

Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the viability of Streptococcus mutans and Aggregatibacter  actinomycetemcomitans using single- and dual-species biofilm at different phases of formation.Methods: Biofilm models were incubated for 4, 12, and 24 hrs, then exposed to the extract at a concentration of 0.525%.Results: The viability of the single-species S. mutans biofilm was low (p<0.05), and no significant difference (p>0.05) was found between singlespeciesA. actinomycetemcomitans and dual-species biofilm.Conclusions: Curcuma xanthorrhiza ethanol extract is more effective for decreasing the viability of single-species S. mutans biofilm.

scholarly journals Expression of biofilm-associated genes of Streptococcus mutans in response to glucose and sucrose

2007 ◽  
Vol 56 (11) ◽  
pp. 1528-1535 ◽  
Author(s):  
Moshe Shemesh ◽  
Avshalom Tam ◽  
Doron Steinberg
Keyword(s):  
Gene Expression ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Fold Increase ◽  
Extracellular Polysaccharide ◽  
Tooth Surface ◽  
Differential Analysis ◽  
Nutrient Contents ◽  
Genes Encoding

Streptococcus mutans is known as a primary pathogen of dental caries, one of the most common human infectious diseases. Exopolysaccharide synthesis, adherence to tooth surface and biofilm formation are important physiological and virulence factors of S. mutans. In vitro comparative gene expression analysis was carried out to differentiate 10 selected genes known to be mostly involved in S. mutans biofilm formation by comparing the expression under biofilm and planktonic environments. Real-time RT-PCR analyses indicated that all of the genes tested were upregulated in the biofilm compared to cells grown in planktonic conditions. The influence of simple dietary carbohydrates on gene expression in S. mutans biofilm was tested also. Among the tested genes, in the biofilm phase, the greatest induction was observed for gtf and ftf, which are genes encoding the extracellular polysaccharide-producing enzymes. Biofilm formation was accompanied by a 22-fold induction in the abundance of mRNA encoding glucosyltransferase B (GTFB) and a 14.8 -fold increase in mRNA encoding GTFC. Levels of mRNA encoding fructosyltransferase were induced approximately 11.8-fold in biofilm-derived cells. Another notable finding of this study suggests that glucose affects the expression of S. mutans GS5 biofilm genes. In spite of a significant upregulation in biofilm-associated gene expression in the presence of sucrose, the presence of glucose with sucrose reduced expression of most tested genes. Differential analysis of the transcripts from S. mutans, grown in media with various nutrient contents, revealed significant shifts in the expression of the genes involved in biofilm formation. The results presented here provide new insights at the molecular level regarding gene expression in this bacterium when grown under biofilm conditions, allowing a better understanding of the mechanism of biofilm formation by S. mutans.

Who will win the race in childrens' oral cavities? Streptococcus mutans or beneficial lactic acid bacteria?

2013 ◽  
Vol 4 (3) ◽  
pp. 237-245 ◽  
Author(s):  
ö.E. Güngör ◽  
Z. Kırzıoğlu ◽  
E. Dinçer ◽  
M. Kıvanç
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Oral Cavity ◽  
Streptococcus Mutans ◽  
Meat Products ◽  
Tooth Surface ◽  
Healthy Children ◽  
Bacterial Strains ◽  
Adhesive Properties ◽  
Meat Samples

Adhesion to oral soft and hard tissue is crucial for bacterial colonisation in the mouth. The aim of this work was to select strains of oral lactic acid bacteria that could be used as probiotics for oral health. To this end, the adhesive properties of some lactic acid bacteria were investigated. Seventeen lactic acid bacteria including two Streptococcus mutans strains were isolated from the oral cavity of healthy children, while other strains were isolated from fermented meat products. The bacterial strains were applied to teeth surfaces covered with saliva or without saliva. A significant diversity in adhesion capacity to teeth surfaces among the lactic acid bacteria was observed. Lactic acid bacteria isolated from the oral cavity adhered the best to teeth surfaces covered with saliva, whereas lactic acid bacteria isolated from fermented meat samples adhered the best to tooth surface without saliva. All strains of lactic acid bacteria were able to reduce the number of S. mutans cells, in particular on saliva-coated tooth surface. Therefore, they might have potential as probiotics for the oral cavity.

Antibacterial Activity In Vitro of the Developed Glass Ionomer Cements

2011 ◽  
Vol 493-494 ◽  
pp. 375-380
Author(s):  
Joanna Karaś ◽  
Andrzej Roman Olszyna ◽  
Ewa Zaczyńska ◽  
Anna Czarny ◽  
Krzysztof Szamałek
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Initial Period ◽  
Control Culture ◽  
Fluoride Release ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Bacterial Strains ◽  
Daily Amount

The purpose of the study was to identify the influence of the amount of fluoride release from glass ionomer cements on survival of bacterial strains responsible for the development of secondary caries. All developed cements had an antibacterial impact on the strains used in the study. The study demonstrated that the cements exhibit greater antibacterial activity againstStreptococcus mutansthanStreptococcus sanguinis. The numbers of bacteria count in cultures on the developed cements were approximately 10 times lower compared to the control culture after 24 h, 48 h, 72 h and 7 days. The developed cements had initially (1-2 days) greater antibacterial impact against the strains used in the study,which corresponds to the greatest daily amount of fluoride released from the cements. Following the initial period, the antibacterial activity of all the cements remained roughly at the same level.

scholarly journals Antibacterial effect of clove (Eugenia aromaticum) oil extracted from clove cigarettes towards Streptococcus mutans

2018 ◽  
Vol 25 (1) ◽  
Author(s):  
Ahmad Alif Wazir Jumali ◽  
Mieke Hemiawati Satari ◽  
Warta Dewi
Keyword(s):  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Herbal Remedy ◽  
Tooth Surface ◽  
Clove Oil ◽  
Cigarette Smokers ◽  
Cariogenic Bacteria ◽  
Experimental Laboratory ◽  
Oil Extract

Streptococcus mutans is a commensal bacteria of the oral cavity, particularly found in dental plaque attached to the tooth surface, and can also found in the saliva, buccal mucosa, tongue, and the gingival sulcus. Clove cigarette contains the clove oil used worldwide as a herbal remedy for a variety of health disorders due to its antibacterial, antifungal, and antiviral properties. The purpose of this study was to determined the antibacterial effect of the clove oil contained in the clove cigarette towards Streptococcus mutans as a cariogenic bacteria. The research was an experimental laboratory, which tested the sensitivity of the Streptococcus mutans bacteria taken from the saliva of 10 clove cigarette smokers towards the clove oil extract of the clove cigarette in-vitro by using the Kirby-Bauer method. The study showed that the clove oil extract of the clove cigarette which contained eugenol has antibacterial towards the growth of Streptococcus mutans, and there was a difference of the antibacterial activity between clove oil extracted from minced and combusted clove cigarette. The conclusion of this study was the clove oil extracted from minced clove cigarette had a better antibacterial effect than the combusted clove cigarette.

scholarly journals Assessment and antimricrobial modulating activity of the extract of Baccharis cinerea DC. from cariri cearense

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Cleberton Torres Santos ◽  
Luiz Eduardo Oliveira Teotônio ◽  
Ana Paula Leite Nascimento ◽  
Darcio Luiz de Sousa Júnior ◽  
Ítalo Mykaell da Silva Benjamin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Chemical Compounds ◽  
Therapeutic Effects ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Ethanol Extracts ◽  
Asteraceae Family

Baccharis cinerea belongs to the Asteraceae family, in Brazil is found in the Northeast and Southeast, occurring in the Caatinga and Mata Atlântica biomes, on the edges of the seasonal forests, board and altitude forests in both regenerating primary and secondary areas. Has proven antimicrobial and antiviral activity and is widely used in folk medicine for its various therapeutic effects and is used as an antiseptic for skin and wound infections, inflammation, diarrhea as well as being used as a purgative. The plants used in the traditional medicine are more and more explored scientifically because they are possible resources of substances with antimicrobial activity in front damage man’s health microorganism. In this context the objective of the study was to investigate the antimicrobial activity, modulator activity of antibiotic and in vitro phytochemical prospection of leaf ethanol extracts. Tests were performed on the bacterial strains of Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 15442) and Escherichia coli (ATCC 10536). The antibacterial activity was analyzed by means determining the Minimum Inhibitory Concentration (MIC). For the evaluation of the modulating activity, the microdilution method of the diluted extract samples with the antibiotic’s amikacin, clindamycin and gentamicin was used. The MIC results were ≥ 1024 μg mL-1 by the bacterial strains. There was a relevance of concentrations in modulation with the antimicrobials tested such as amikacin and gentamicin, there were no discrepancy of clindamycin results in association with the extract. The chemical constituents found were leucoanthocyanidins, flabobenic tannins, flavanones, flavones, flavonoids, xanthones, chalcones, aurones. It is important to note that is necessary to do other studies to evaluate the potential of this species because it has important chemical compounds in reducing antimicrobial resistance.

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