Avaliação in vitro da Atividade Antimicrobiana de Extrato Alcoólico de Própolis Comparado à Solução de Clorexidina 0,12%

Apesar dos esforços e do amplo entendimento sobre a cárie dental, está continua bastante prevalente em determinados segmentos da população. A doença apresenta etiologia multifatorial, com a dieta e os micro-organismos envolvidos desenvolvendo papéis fundamentais. Os Streptococcus mutans têm papel fundamental em seu início e é ubíquo em crianças a partir dos sete anos de idade. Para controlar essas bactérias é preciso uma dieta com baixo consumo de sacarose e de carboidratos fermentáveis, o que nem sempre se consegue. Sua remoção da superfície dentária só é possível por meio da fricção mecânica, mas pode-se prevenir sua presença no biofilme dentário através da utilização de antissépticos. O mais utilizado na Odontologia é a clorexidina, porém como não é isenta de efeitos adversos, atualmente, vêm-se estudando produtos alternativos, dentre os quais alguns agentes fitoterápicos. O objetivo deste trabalho foi analisar a atividade da própolis contra os S. mutans em relação à clorexidina. Foi analisada própolis 70% diluída em álcool 96% e após diluída em água potável 5ml, própolis 50%, própolis 12% e clorexidina 0,12% (controle positivo), e solução salina (controle negativo). O operador foi cego em relação às substâncias que estava utilizando. Somente a clorexidina 0.12% e a própolis 12% apresentaram halo de inibição claro. Neste estudo, a clorexidina apresentou melhor atividade contra os S.mutans.Palavras-chave: Clorexidina. Cárie Dentária. Streptococcus mutans.AbstractDespite the efforts and the broad understanding on dental caries, it is still quite prevalent in certain segments of the population. The disease shows a multifactorial etiology, with diet and microorganisms involved developing fundamental roles. The Streptococcus mutans has key role in its beginning and is ubiquitous in children as young as seven years of age. To control these bacteria a diet is necessary with low consumption of sucrose and of fermentable carbohydrates, which is not always possible. The tooth surface removal is only possible through mechanical friction, but it is possible to prevent the presence of dental biofilm through the use of antiseptics. The most widely used in dentistry is chlorhexidine, however as it is not devoid of adverse effects, currently alternative products have been studying, among which some herbal agents. The aim of this study was to analyze the activity of propolis against S. mutans compared to chlorhexidine. Propolis was analyzed diluted in 70% ethanol 96% and further diluted in drinking water 5ml, 50% propolis, propolis 12% and 0.12% chlorhexidine (positive control), and saline (negative control). The operator was blinded for the substances he was using. Only 0.12% chlorhexidine and 12% propolis showed clear inhibition halo. In this study the chlorhexidine presented better activity against S. mutans.Keywords: Chlorhexidine. Dental Caries. Antimicrobials. Streptococcus mutans

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The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

DENTA ◽

10.30649/denta.v10i1.37 ◽

2016 ◽

Vol 10 (1) ◽

pp. 71

Author(s):

Almira Fa’Izah ◽

Istien Wardani ◽

Diana Soesilo

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Control Group ◽

Oral Diseases ◽

Clear Zone ◽

Control Group Design

Background: Dental and oral diseases which are often found in children is dental caries. Streptococcus mutans is the main cause of caries. Caries can be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) within the anchovy can inhibit the occurrence of dental caries. Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. Materials and Methods: This study was a laboratory experimental research with post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimirobial zone diameter. The average zone of antimicrobial at the concentration of 3% were 7,11 mm, 6% 9,5 mm, 12% 10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P < 0,05). Conclusion: The anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.

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The anti-adherence effect of Lippia sidoides Cham: extract against microorganisms of dental biofilm

Revista Brasileira de Plantas Medicinais ◽

10.1590/s1516-05722013000100005 ◽

2013 ◽

Vol 15 (1) ◽

pp. 41-46 ◽

Cited By ~ 3

Author(s):

A.C.L. Albuquerque ◽

M.S.V. Pereira ◽

D.F. Silva ◽

L.F. Pereira ◽

F.A.C. Viana ◽

...

Keyword(s):

Streptococcus Mutans ◽

Folk Medicine ◽

Tooth Surface ◽

Bacterial Strains ◽

Lippia Sidoides ◽

Dental Biofilm ◽

Scientific Exploration ◽

Infectious Origin ◽

In Vitro Simulation

Most illnesses affecting the oral cavity are proven to have infectious origin. Several categories of chemical agents have been used in the chemical control of dental biofilm through strategies that aim at reducing bacterial adhesion and inhibiting the growth and the proliferation of microorganisms on the tooth surface. The use of plants in folk medicine and in Dentistry, as well as the spread of successful cases, has led to scientific exploration, resulting in chemical-pharmacological knowledge of thousands of plants. The present study aimed to evaluate the anti-adherence activity of Lippia sidoides Cham., comparing the results with those of 0.12% chlorhexidine by means of an in vitro simulation of dental biofilm. The studied bacterial strains were Streptococcus mutans, Streptococcus sanguinis and Lactobacillus casei, main responsible for the biofilm adherence. The studied extract was effective in inhibiting the adherence of Streptococcus mutans up to a concentration of 1:16, compared to Chlorhexidine. Lippia sidoides Cham extract showed anti-adherence effect on the major microorganisms responsible for dental biofilm consolidation.

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Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

Revista de Biología Tropical ◽

10.15517/rbt.v66i4.33140 ◽

2018 ◽

Vol 66 (4) ◽

pp. 1519

Author(s):

Mailen Ortega Cuadros ◽

Adriana Patricia Tofiño Rivera ◽

Luciano Jose Merini ◽

Maria Cecilia Martinez Pabon

Keyword(s):

Antimicrobial Activity ◽

Dental Caries ◽

Essential Oil ◽

Streptococcus Mutans ◽

Human Fibroblasts ◽

Positive Control ◽

Negative Control ◽

Cymbopogon Citratus ◽

Significant Difference ◽

Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

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AN INVITRO COMPARATIVE EVALUATION OF ANTICANDIDAL HERBS (GINGER & TURMERIC) ON STREPTOCOCCUS MUTANS

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i1.76 ◽

2019 ◽

Vol 3 (1) ◽

Author(s):

Bafna Harshal P ◽

Lunawat Sadhana R ◽

Vathar Jagdishchandra ◽

Kalantharakath Thanveer ◽

Ricky Pal Singh ◽

...

Keyword(s):

Streptococcus Mutans ◽

Ethanolic Extract ◽

Antimicrobial Effect ◽

Positive Control ◽

Negative Control ◽

Dimethyl Formamide ◽

Zone Of Inhibition ◽

Ginger Extract ◽

Inert Solvent

Aim: To comparatively evaluate the antimicrobial effect of turmeric and ginger extracts on Streptococcus mutans in in-vitro conditions. Material & Method: An in-vitro experimental study was conducted in a laboratory setting. Ethanolic extract of Ginger and Turmeric was prepared separately by cold masseration technique. The extract of each was then diluted with an inert solvent, Dimethyl Formamide, to obtain 5 different concentrations (2%, 4%, 6%, 8%, and 10%) of each. 0.2% chlorhexidine was used as a positive control and dimethyl formamide was used as negative control. The different extracts, along with controls, were then subjected to microbiological investigation to determine which gave a wider zone of inhibition against streptococcus mutans. The zone of inhibition was measured in millimeters. Results: Turmeric extracts presented the largest zone of inhibition 33mm at the concentration of 8%, while Ginger extract showed a zone of inhibition of 34mm at the concentration of 10%. Conclusion: Ethanolic extract of Turmeric demonstrated antimicrobial activity against Strptococcus Mutans at a lower concentration than that of Ginger. Keywords: Ginger, Turmeric, Streptococcus Mutans.

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Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization

The Open Dentistry Journal ◽

10.2174/1874210602014010544 ◽

2020 ◽

Vol 14 (1) ◽

pp. 544-553

Author(s):

Armelia Sari Widyarman ◽

Triska Ramajayanti ◽

Citra Fragrantia Theodorea

Keyword(s):

Streptococcus Mutans ◽

Biofilm Formation ◽

Lactobacillus Reuteri ◽

Positive Control ◽

Negative Control ◽

Microbial Biofilms ◽

Incubation Periods ◽

Negative Controls ◽

Initial Biofilm

Background: The benefits of probiotics for human health have long been proven. Probiotic Lactobacillus reuteri, can produce a beneficial broad-spectrum antibacterial compound called reuterin by metabolizing glycerol. Objective: The aim of the study was to investigate the effect of the Indonesian strain of L. reuteri LC382415 on mono- and dual-species Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Methods: Streptococcus mutans and S. sanguinis were cultured in BHI broth. Lactobacillus reuteri LC382415 was inoculated on MRS agar. The different concentrations effect of L. reuteri (1×104, 1×106, and 1×108 CFU/mL) with and without glycerol supplementation on microbial biofilms were examined using a biofilm assay after incubation for 1,3,6, and 24-h. The biofilm mass optical density was measured with a microplate spectrophotometer at 490 nm. Chlorhexidine gluconate (0.2%) was used as a positive control, and wells without treatment were used as negative controls. Results: A significant reduction in mono- and dual-species S. mutans and S. sanguinis biofilm formation was observed after treatment with all concentrations of L.reuteri and after all incubation periods (p<0.05) with or without glycerol supplementation. The concentration of 1×104 CFU/mL after 3-h incubation was the most effective in inhibiting biofilm formation, with 87.8% S. mutans, 95.9% S. sanguinis, and 80.4% dual-species biofilm reduction compared to the negative control (p<0.05). Conclusion: The Indonesian strain of L. reuteri effectively reduces mono- and dual-species S.mutans and S. sanguinis biofilms. This suggests that it may be useful in preventing biofilm formation in oral cavities. Future studies on the mechanism of action of this active component are warranted.

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INHIBITION TEST OF METHANOL EXTRACT FROM SOURSOP LEAF (Annona muricata Linn.) AGAINST Streptococcus mutans BACTERIA*

Jurnal Natural ◽

10.24815/jn.v17i1.6823 ◽

2017 ◽

Vol 17 (1) ◽

pp. 23 ◽

Cited By ~ 2

Author(s):

Raudhatul Jannah ◽

Muhammad Ali Husni ◽

Risa Nursanty

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Methanol Extract ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Annona Muricata ◽

High Prevalence ◽

Chemical Content ◽

And Mathematics

Abstract. Dental caries is a disease with a high prevalence of caries in Indonesia is caused by the bacterium Streptococcus mutans. The leaves of the soursop (Annona muricata Linn.) is one of the herbs that can remove dental plaque. This study aims to determine the chemical content and measure the inhibitory antibacterial soursop leaft methanol extractin inhibiting the growth of Streptococcus mutans bacteria. Phytochemical screening stated that the leaf of the soursop contains flavonoids, alkaloids, tannins, steroids, glycosides and saponins. Test of inhibition were measured using paper disc diffusion method with variation concentrations of 5, 10, 15 20, and 25%, positive control is tetrasilkin 30 µg, and a negative control is methanol. Antibacterial test results showed that the methanol extract of soursop leaves have antibacterial activity against Streptococcus mutans at concentrations of 5, 10, 15, 20 and 25% with inhibitory diameter of 9,1; 10,57; 11,53; 12,01 and 13,75 mm respectively. Keywords: Soursop leaves, Dental caries, Streptococcus mutans, Methanol.*Judul ini telah dipresentasikan pada Seminar Nasional: Indonesian Students Conference on Science and Mathematics (ISCSM) 11-12 November 2015, Banda Aceh Indonesia

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Potensi Ekstrak Buah Delima Merah (Punica granatum Linn) terhadap Penurunan Jumlah Koloni Streptococcus mutans

Pustaka Kesehatan ◽

10.19184/pk.v6i2.8655 ◽

2018 ◽

Vol 6 (2) ◽

pp. 351

Author(s):

Kholisa Kholisa ◽

Purwanto Purwanto ◽

Sri Hernawati

Keyword(s):

Streptococcus Mutans ◽

Punica Granatum ◽

Negative Control Group ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Tooth Surface ◽

Control Group ◽

Study Group ◽

Plaque Ph

Streptococcus mutans is a bacteria that initiates the formation of plaque on the tooth surface. S. mutans works by fermenting carbohydrates to produce an acidic atmosphere, so the plaque pH becomes low, this condition can lead to demineralization of enamel and dentin commonly called caries. One of alternative treatment to reduce the population of S. mutans by using herbal plants, namely red pomegranate. Red pomegranate contains flavonoids, tannins, and alkaloids as antibacterial. This study aimed to determine the inhibition of red pomegranate extract on growth of S. mutans. The method used is by counting the number of S. mutans using a colony counter. This method with 4 samples in each study group. The study group consists of 4 treatment groups (25%, 50%, 75% and 100% red pomegranate extract), positive control group (chlorhexidine), and negative control group (sterile aquades). Data were analyzed using Kruskal-Wallis Test and Mann-Whitney test. Red pomegranate extract has the ability to inhibit the growth of S. mutans. The concentration of red pomegranate extract that has the greatest inhibitory effect on S. mutans growth is 100%. Keyword: Antibacterial, Streptococcus mutans, caries, red pomegranate fruit extract

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EFFECT OF CURCUMA XANTHORRHIZA ROXB. ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS DUAL-SPECIES BIOFILMS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s1.181 ◽

2019 ◽

pp. 75-78

Author(s):

FARIDA ERVINTARI ◽

RIA PUSPITAWATI ◽

SRI UTAMI

Keyword(s):

Streptococcus Mutans ◽

Bacterial Culture ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Streptococcus Sanguinis ◽

Curcuma Xanthorrhiza ◽

Ethanol Extracts ◽

Negative Controls

Objective: This study aimed to determine the effect of ethanol Curcuma extract on the viability of S. mutans and Streptococcus sanguinis in a dualspeciesin vitro biofilm model.Methods: Dual-species biofilms of S. mutans and S. sanguinis were exposed to ethanol Curcuma extract at various concentrations. The sample ofsaliva was gathered from healthy volunteers. Chlorhexidine 0.2% was used as a positive control, and bacterial culture without intervention servedas a negative control. The total suspensions of 10−as were prepared for S. mutans and S. sanguinis cells. The bacteria were incubated for 20 h (activematuration phase) and 24 h (maturation phase).Results: The result showed decreased S. mutans and S. sanguinis viability after exposure to 0.2%–25% Curcuma ethanol extracts during the activeaccumulation and maturation phases. The decrease in bacterial viability was significantly different in all concentrations of Curcuma ethanol extractscompared with negative controls (p<0.05) in the active accumulation and maturation phases.Conclusion: Temulawak ethanol extract (starting at 0.2%) can decrease the viability of S. mutans and S. sanguinis in a dual species in vitro biofilmmodel during the accumulation and maturation phases.

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Novel Antibiofilm Chemotherapy Targets Exopolysaccharide Synthesis and Stress Tolerance in Streptococcus mutans To Modulate Virulence ExpressionIn Vivo

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01381-12 ◽

2012 ◽

Vol 56 (12) ◽

pp. 6201-6211 ◽

Cited By ~ 41

Author(s):

Megan L. Falsetta ◽

Marlise I. Klein ◽

José A. Lemos ◽

Bruno B. Silva ◽

Senyo Agidi ◽

...

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Positive Control ◽

Sprague Dawley ◽

Content Type ◽

Treatment Regimens ◽

Novel Target ◽

Underlying Mechanisms

ABSTRACTFluoride is the mainstay of dental caries prevention, and yet current applications offer incomplete protection and may not effectively address the infectious character of the disease. Therefore, we evaluated the effectiveness of a novel combination therapy (CT; 2 mM myricetin, 4 mMtt-farnesol, 250 ppm of fluoride) that supplements fluoride with naturally occurring, food-derived, antibiofilm compounds. Treatment regimens simulating those experienced clinically (twice daily for ≤60 s) were used bothin vitroover a saliva-coated hydroxyapatite biofilm model andin vivowith a rodent model of dental caries. The effectiveness of CT was evaluated based on the incidence and severity of carious lesions (compared to fluoride or vehicle control). We found that CT was superior to fluoride (positive control,P< 0.05); topical applications dramatically reduced caries development in Sprague-Dawley rats, all without altering theStreptococcus mutansor total populations within the plaque. We subsequently identified the underlying mechanisms through which applications of CT modulate biofilm virulence. CT targets expression of keyStreptococcus mutansgenes during biofilm formationin vitroandin vivo. These are associated with exopolysaccharide matrix synthesis (gtfB) and the ability to tolerate exogenous stress (e.g.,sloA), which are essential for cariogenic biofilm assembly. We also identified a unique gene (SMU.940) that was severely repressed and may represent a potentially novel target; its inactivation disrupted exopolysaccharide accumulation and matrix development. Altogether, CT may be clinically more effective than current anticaries modalities, targeting expression of bacterial virulence associated with pathogenesis of the disease. These observations may have relevance for development of enhanced therapies against other biofilm-dependent infections.

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The inhibition of Streptococcus mutans glucosyltransferase enzyme activity by mangosteen pericarp extract

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v50.i2.p97-101 ◽

2017 ◽

Vol 50 (2) ◽

pp. 97 ◽

Cited By ~ 1

Author(s):

Nirawati Pribadi ◽

Yovita Yonas ◽

Widya Saraswati

Keyword(s):

Enzyme Activity ◽

Dental Caries ◽

Streptococcus Mutans ◽

High Performance ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Inhibitory Power ◽

Glucan Synthesis

Background: Streptococcus mutans (S. mutans) is a bacterium that plays an important role in the pathogenesis of dental caries. Streptococcus mutans produces the glucosyltransferase enzyme which is capable of catalyzing glucan synthesis in the progression of dental caries. Certain treatments involving traditional plant use have been developed to eradicate Streptococcus mutans as a means of preventing the formation of dental caries. One of these is mangosteen pericarp extract containing a number of polyphenols that have the capacity to act as antibacterial agents, namely; tannin, mangostin, and flavonoid. Purpose: The research aimed to investigate the inhibitory power of mangosteen pericarp extract against Streptococcus mutans producing the glucosyltransferase enzyme. Methods: The research used mangosteen pericarp extract at concentrations of 0.39% and 0.78% as the treatments, while 0.12% chlorhexidine gluconate was used as a positive control, and distilled water as a negative control. Each group consisted of six samples. Mangosteen peels extracted with 96% ethanol (maceration method) and mangosteen extract constituted 5% of the total weight of the mangosteen pericarp. Supernatant containing Gtf enzyme produced from a culture medium and centrifuged at 1500 rpm for 10 minutes at 4o C. Glucosyltransferase enzyme activity was measured by analyzing the extensive fructose area by means of High Performance Liquid Chromatography (HPLC). The extensive fructose area was determined according to time retention in each group. Results: Mangosteen peel extract at concentrations of 0.39% and 0.78% demonstrated greater ability than the negative control group (sterile aquades) and similar ability to the positive group (chlorhexidine 0.12%) to inhibit the activity of the Gtf enzyme or S. mutans bacteria. Conclusion: Mangosteen pericarp extract has the ability to inhibit the activity of Streptococcus mutans in producing glucosyltransferase enzyme.

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