Drying temperature affects rice seed vigor via gibberellin, abscisic acid, and antioxidant enzyme metabolism

Sunflower seed storage is accompanied by the loss of seed vigor. Seed drying is a key link between seed harvest and seed storage; however, to date, the effect of seed drying on sunflower seed deterioration during storage remains unclear. The present study performed hot air drying for sunflower seeds with an initial moisture content of 30% to examine the manner in which drying temperature (35, 40, 45, 50, and 55°C) affects the drying performance and seed vigor following storage process (6 and 12 months). A drying temperature of 40°C was evidently safe for sunflower seeds, whereas the high drying temperatures (HTD, 45, 50, and 55°C) significantly lowered sunflower seed vigor by regulating the fatty acid metabolism, glycometabolism, and abscisic acid (ABA)/gibberellin (GA) balance. HDT significantly increased the seed damage rate and accelerated sunflower seed deterioration during natural and artificial aging process. Further biochemical analysis indicated that HDT significantly increased lipoxygenase and dioxygenase activities, leading to malonaldehyde and reactive oxygen species over-accumulation during storage. During early seed germination, HDT significantly inhibited fatty acid hydrolysis and glycometabolism by decreasing triacylglycerol lipase, CoA-SH oxidase, and invertase activities. Moreover, HDT remarkably increased ABA levels but reduced GA levels by regulating gene expressions and metabolic enzyme activities during early imbibitions. Cumulatively, the seed drying effect on sunflower seed vigor deterioration during the storage process may be strongly related to fatty acid oxidation and hydrolysis metabolism, toxic substance accumulation, and ABA/GA balance.

Download Full-text

OsGRETCHENHAGEN3-2 modulates rice seed storability via accumulation of abscisic acid and protective substances

Plant Physiology ◽

10.1093/plphys/kiab059 ◽

2021 ◽

Author(s):

Zhiyang Yuan ◽

Kai Fan ◽

Yuntong Wang ◽

Li Tian ◽

Chaopu Zhang ◽

...

Keyword(s):

Abscisic Acid ◽

Molecular Mechanisms ◽

Genome Wide Association Study ◽

Seed Viability ◽

Seed Vigor ◽

Negative Regulator ◽

Artificial Ageing ◽

Rice Seed ◽

A Genome ◽

Seed Storability

Abstract Seed storability largely determines the vigor of seeds during storage and is significant in agriculture and ecology. However, the underlying genetic basis remains unclear. In the present study, we report the cloning and characterization of the rice (Oryza sativa) indole-3-acetic acid (IAA)-amido synthetase gene GRETCHEN HAGEN3-2 (OsGH3-2) associated with seed storability. OsGH3-2 was identified by performing a genome-wide association study in rice germplasms with linkage mapping in chromosome substitution segment lines, contributing to the wide variation of seed viability in the populations after long periods of storage and artificial ageing. OsGH3-2 was dominantly expressed in the developing seeds and catalyzed IAA conjugation to amino acids, forming inactive auxin. Transgenic overexpression, knockout and knockdown experiments demonstrated that OsGH3-2 affected seed storability by regulating the accumulation level of abscisic acid. Overexpression of OsGH3-2 significantly decreased seed storability, while knockout or knockdown of the gene enhanced seed storability compared with the wild type. OsGH3-2 acted as a negative regulator of seed storability by modulating many genes related to the abscisic acid pathway and probably subsequently late embryogenesis-abundant proteins at the transcription level. These findings shed light on the molecular mechanisms underlying seed storability and will facilitate the improvement of seed vigor by genomic breeding and gene-editing approaches in rice.

Download Full-text

CSN improves seed vigor of aged sunflower seeds by regulating fatty acid, glycometabolism, and abscisic acid metabolism

Journal of Advanced Research ◽

10.1016/j.jare.2021.01.019 ◽

2021 ◽

Author(s):

Yutao Huang ◽

Shuyu Cai ◽

Xiaoli Ruan ◽

Jun Xu ◽

Dongdong Cao

Keyword(s):

Abscisic Acid ◽

Fatty Acid ◽

Acid Metabolism ◽

Seed Vigor ◽

Sunflower Seeds ◽

Abscisic Acid Metabolism

Download Full-text

Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

Annual Research & Review in Biology ◽

10.9734/arrb/2019/v32i130075 ◽

2019 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

S. N. Mahadi ◽

F. Zawawi ◽

R. Nulit ◽

M. H. Ibrahim ◽

N. I. Ab. Ghani

Keyword(s):

Oryza Sativa ◽

Seed Germination ◽

Germination Percentage ◽

Leaf Length ◽

Seed Vigor ◽

Rice Seed ◽

Combination Treatments ◽

Randomized Design ◽

Completely Randomized Design ◽

Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

Download Full-text

Coumarin-Induced Delay of Rice Seed Germination Is Mediated by Suppression of Abscisic Acid Catabolism and Reactive Oxygen Species Production

Frontiers in Plant Science ◽

10.3389/fpls.2019.00828 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 3

Author(s):

Bing-Xian Chen ◽

Yuan-Xuan Peng ◽

Jia-Dong Gao ◽

Qi Zhang ◽

Qin-Jian Liu ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Abscisic Acid ◽

Seed Germination ◽

Reactive Oxygen Species Production ◽

Reactive Oxygen ◽

Rice Seed ◽

Oxygen Species ◽

Species Production

Download Full-text

Rice Seed Vigor and Seedling Establishment in Anaerobic Soil

Crop Science ◽

10.2135/cropsci1996.0011183x003600030027x ◽

1996 ◽

Vol 36 (3) ◽

pp. 680-686 ◽

Cited By ~ 50

Author(s):

M. Yamauchi ◽

Tun Winn

Keyword(s):

Seedling Establishment ◽

Seed Vigor ◽

Rice Seed

Download Full-text

Effect of High Temperature Intermittent Drying on Rice Seed Viability and Vigor

International Journal of Food Engineering ◽

10.1515/ijfe-2016-0433 ◽

2017 ◽

Vol 13 (10) ◽

Author(s):

Pan Wang ◽

Dong Li ◽

Li-jun Wang ◽

Benu Adhikari

Keyword(s):

High Temperature ◽

Ambient Temperature ◽

Seed Viability ◽

Seed Vigor ◽

Drying Rate ◽

Rice Seed ◽

Rice Cultivars ◽

Moisture Contents ◽

Rice Seeds ◽

Tempering Process

AbstractThis work aimed at determining whether high temperature intermittent drying followed by tempering at ambient temperature could preserve the seed viability and vigor.JaponicaandIndicarice seeds with 21.2 % and 22.6 % wet moisture contents (w.b.) were dried at 50 ºC and 60ºC for either 5, 10, 15 or 20 minutes, followed by tempering at 25ºC for 45 minutes. Each drying cycle was repeated until the rice seeds were dried to 12.0 % (w.b.). The drying rate was improved and the total in-dryer time was reduced in the intermittent drying when compared to continuous drying, due to the tempering process. The seed vigor was significantly reduced by intermittent drying at 60 °C with all exposure times, although the seed still kept the ability to germinate for both rice cultivars. The intermittent drying at 50 ºC for 5 minutes per drying cycle preserved the seed vigor ofJaponicarice well, while inIndicarice, the intermittent drying at 50 ºC up to 20 minutes could still be able to maintain the seed vigor.

Download Full-text

Pengujian Vigor Daya Simpan dan Vigor terhadap Kekeringan pada Benih Padi Gogo dan Padi Sawah

Buletin Agrohorti ◽

10.29244/agrob.1.1.67-71 ◽

2013 ◽

Vol 1 (1) ◽

pp. 67

Author(s):

Feni Shintarika ◽

Faiza Chairani Suwarno ◽

. Suwarno

Keyword(s):

Drought Stress ◽

Upland Rice ◽

Block Design ◽

Seed Vigor ◽

Lowland Rice ◽

Rice Seed ◽

Ethanol Vapour ◽

Randomized Block Design ◽

Rice Genotypes ◽

Seed Storability

<p style="text-align: justify;">The objectives of the study were to evaluate seed storability and vigor to drought of rice genotypes from different ecologies and correlation among the characters. The experiments were conducted in the Seed Science and Technology Laboratory, Bogor Agricultural University from April to August 2011. Duration of rapid aging using ethanol vapour for testing seed storability were 87.6 and 155.4 minutes for upland and lowland rice seeds respectively. The rolled paper methods with Polyethylen Glycol (PEG 6000) at -2.0 bar was used for testing seed vigor to drought. Rice seed of 40 genotype consisting of 20 upland and 20 lowland were evaluated using Randomized Block Design with three replications. There were no correlation between seed storability and vigor to drought on all genotypes, and no similiarity of those characters on the best five genotypes. Genotypes with the highest storability were B12154D-MR-22-8 for upland rice and Aek Sibundong for lowland. Genotypes with the highest vigor to drought were B12165D-MR-8-1 and B12161D-MR-1-4-2 for upland rice, B12672-MR-19-2-PN-1-3 and Aek Sibundong for lowland.</p><p>Keywords : drought stress, Rapid aging</p>

Download Full-text

ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

Revista Caatinga ◽

10.1590/1983-21252021v34n406rc ◽

2021 ◽

Vol 34 (4) ◽

pp. 791-798

Author(s):

RAFAEL MARANI BARBOSA ◽

MATHEUS ANDRÉ DE JESUS ◽

RAFAELA ALVES PEREIRA ◽

GEDEON ALMEIDA GOMES JUNIOR

Keyword(s):

Electrical Conductivity ◽

Efficient Method ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Vigor ◽

Rice Seed ◽

Seed Sample ◽

Red Rice ◽

Germination Test ◽

Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

Download Full-text