scholarly journals Rice vinegar removes Candida albicans from denture acrylic resins

2021 ◽  
Vol 76 (07) ◽  
pp. 409-413
Author(s):  
Tarana Garach ◽  
Sindisiwe Shangase ◽  
Mulalo Molaudzi ◽  
Julitha Molepo
Keyword(s):  
Candida Albicans ◽  
Low Cost ◽  
Acrylic Resin ◽  
White Wine ◽  
Denture Stomatitis ◽  
Significance Level ◽  
Apple Cider ◽  
Acrylic Resins ◽  
Post Test ◽  
Rice Vinegar

Denture stomatitis, mainly caused by Candida albicans, often affect denture wearers. To manage these patients, denture hygiene is of utmost importance. There is a need for low cost, easily accessible denture disinfectants. To investigate the efficacy of rice vinegar and other disinfecting solutions in removing C. albicans from acrylic resins. Hundred and eighty acrylic resin plates were contaminated with C. albicans strains and divided into five groups. These were immersed in apple cider vinegar (ACV), white wine vinegar (WWV), rice vinegar (RV), chlorhexidine (CHX), and sterile distilled H2O (control). The plates were incubated at room temperature for 30 minutes, 1 hour and 8 hours. Candida removing ability of the disinfecting solutions was evaluated, and data was analyzed using two-way ANOVAwith Tukey post-test. Significance level of p< 0.05 was used. RV, ACV, WWV and CHX showed the highest efficacy (100%) in removing both C. albicans strains at 8 hours (p>0.05). CHX was the most effective disinfectant in removing both C. albicans strains at 30 minutes, 1 hour, and 8 hours (99%-100%). RV was as effective as ACV, WWV and CHX in removing C. albicans from acrylic plates at 8 hours.

scholarly journals The Effectivity of Parsley (Petroselinum crispum) Extract on The Growth Inhibition of Candida Albicans

2021 ◽  
Vol 7 (3) ◽  
pp. 123-128
Author(s):  
Rafinda Naditya Arismunandar ◽  
Isniya Nosartika ◽  
Brigitta Natania Renata Purnomo ◽  
Arlita Leniseptaria Antari
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
Acrylic Resin ◽  
Etiologic Agent ◽  
Control Group ◽  
Petroselinum Crispum ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Inhibition Of Growth ◽  
Post Test

Background: Denture stomatitis is a type of Candida-associated infection that mainly affects the palatal mucosa. Candida albicans is one of the normal flora that is considered to be the primary etiologic agent in the pathogenesis of denture stomatitis. Denture decontamination is necessary to prevent denture stomatitis. One method of decontamination is by immersing removable dentures in an antifungal solution. Parsley (Petroselinum crispum) is a medicinal plant showing antifungal activity. Objectives: To determine the effectivity of immersion of acrylic resin Plate in parsley extract on inhibition of growth of Candida albicans. Methods: The effectivity of immersion of heat-cured acrylic resin plates in parsley extract on inhibition of C. albicans growth was tested using an experimental laboratory study with a post-test-only control group design. Thirty samples were divided into 5 groups as 0.01%, 0.02%, and 0.05% parsley ethanol extract, 0.1% sodium hypochlorite, and sterile aquadest.  The number of colony forming units per mL was obtained after calculating the colonies on SDA media, allowing the minimum inhibitory concentration (MIC) obtained according to the formula. Results: 0.01% parsley ethanol extract is MIC with a mean of 7.4 CFU / ml, which inhibited the growth of C. albicans by 31.05%. The Kruskal Wallis test (p<0.001) results indicate that there is an effectivity of immersion of acrylic resin plate in parsley extract on inhibition of growth of C. albicans. Conclusions: The immersion of acrylic resin plates in parsley ethanol extract with a concentration of 0.01%, 0.02%, and 0.04% has effectivity on the inhibition of growth of C. albicans.

Evaluation of Antibacterial and Antifungal Properties of ZnO and Silver Nanoparticles against Candida Albicans and Streptococcus Mutans in Acrylic Resin

2021 ◽  
Author(s):  
Amin Khaleghi ◽  
Amin Archin ◽  
Mojtaba Azarian Borujeni
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Zno Nanoparticles ◽  
Acrylic Resin ◽  
Spherical Particles ◽  
Significance Level ◽  
Ultrasonic Apparatus ◽  
Experimental Laboratory ◽  
Antibacterial And Antifungal ◽  
Post Hoc

Introduction: This study aimed to evaluate the antibacterial and antifungal effect of Ag and zinc oxide nanoparticles to complete denture acrylic resin. Materials & Methods: In this experimental laboratory study, 144 samples were evaluated. The powder of acrylic resin (PMMA Ivoclar Vivadent, Lichenstein SR Triplex Hot) was mixed homogeneously in the ultrasonic apparatus with Ag (purity: 99.99%, size: 20nm, spherical) and ZnO (purity: 99%, size: 10-30 nm, nearly spherical) particles with mass fraction 0.5, 1 ,2 and 5. The microorganisms of this study were Candida albicans and Streptococcus mutans. The growth rate of microorganisms was measured by spectrophotometer based on turbidity and readings at 600 nm. Data were analyzed by ANOVA and Tukey post hoc tests. The significance level was set at 0.05. Results: Increasing Ag and ZnO nanoparticles to acrylic resin reduced the growth of Candida Albicans and Streptococcus Mutans. Even the concentration of 0.5% significantly reduced the growth of microorganisms. Complete growth inhibition was in the concentration of 5% after 24 and 48 hours. Conclusion: Increasing the Ag and ZnO nanoparticles to acrylic resin reduced the growth of microorganisms.

scholarly journals Acrylic resin disinfection by peracetic acid and microwave energy

2015 ◽  
Vol 63 (3) ◽  
pp. 315-318 ◽  
Author(s):  
Carmen Beatriz Borges FORTES ◽  
Vicente Castelo Branco LEITUNE ◽  
Fabrício Mezzomo COLLARES ◽  
Nélio Bairros DORNELLES JUNIOR ◽  
Stéfani Becker RODRIGUES ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Microwave Irradiation ◽  
Incubation Period ◽  
Peracetic Acid ◽  
Acrylic Resin ◽  
Microwave Energy ◽  
The Other ◽  
Disinfection Methods ◽  
Acrylic Resins ◽  
Irradiation Power

Objective: The objective of this study was to evaluate the effectiveness of disinfection methods in microwave and immersion in peracetic acid in heat-cured, self-cured and microwave-cured acrylic resin, contaminated with Candida albicans. Methods: Five specimens were prepared for each type of acrylic resin. All were infected with Candida Albicans, incubated at 37°C for 24 hours. The group which underwent microwave energy was irradiated with a power of 840W for 1 minute and the other group underwent disinfection by soaking of 0.2% peracetic acid for 5 minutes. Results: All samples proved to be contaminated after the incubation period. After the different processes of disinfection, both immersion in 0.2% peracetic acid as microwave irradiation were effective in disinfection of the 3 types of acrylic resins contaminated by Candida Albicans. Conclusion: Concluded that soaking in 0,2% peracetic acid for 5 minutes with microwave irradiation power 840W for 1 minute are effective methods for disinfecting heat-cured acrylic resin, self-cured acrylic resin and microwave-cured acrylic resin, contaminated with Candida Albicans.

scholarly journals JUMLAH KOLONI CANDIDA ALBICANS PADA PLAT RESIN AKRILIK HEAT CURED SETELAH DILAKUKAN PERENDAMAN EKSTRAK DAUN KEMANGI (OCIMUM BASSILICUM LINN) 50%

2020 ◽  
Vol 16 (2) ◽  
pp. 61-63
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
Sintha Nugrahini
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Parametric Test ◽  
Group Design ◽  
Cleaning Methods

Accumulation of plaque and food scraps on an acrylic resin base will increase bacterial colonies and C. albicans which will cause denture stomatitis. Maintenance of denture hygiene using mechanical, chemical and combination of two methods. Basil leaf extract contain essential oils which are important in fight against resistant C. albicans biofilms. The purpose of this study was to determine the comparison of C. albicans colonies after cleaning various denture cleaning methods. The method of this study is used an experimental method, the study design is posttest only with control group design. Sample size of heat-cured acrylic resin plate is 40x12x3mm. this study use Kruskall-Wallis test and Mann-Whitney test as data analysis for comparison tests between groups (non-parametric test). The results showed that there were significant differences between the various cleansing methods used in reducing C. albicans colonies. The compotition of flavonoids basil leaf extract is anti-microbial which can prevent the entry of fungi that harm the body.

scholarly journals Chitosan 2% Effect on Prohibiting the Growth of Candida Albicans on Heatcured Acrylic Resin

2017 ◽  
Vol 6 (2) ◽  
pp. 17
Author(s):  
Angela Evelyna ◽  
Dahlia Sutanto ◽  
Elizabeth Tiffany
Keyword(s):  
Candida Albicans ◽  
Statistical Analysis ◽  
Natural Compound ◽  
Agar Medium ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Base Materials ◽  
Oral Tissue ◽  
The Difference

Maintaining acrylic denture hygiene is a necessary to prohibit the growth of several oral fungi such as Candida albicans. Candida albicans are the main etiological microorganism of denture stomatitis. One of the ways to clean denture is by immersion on synthetic denture cleaner liquid such as oxygenizing denture cleaners. However, synthetic denture cleaner have several limitation due to its biocompatibility on human body especially oral tissue. Chitosan is a derivate of chitin, a natural compound of sea creature exosceleton. Chitosan have antifungi and antibacterial nature. The objective of this study is to evaluate the antifungi efectifity of chitosan 2% on acrylic heatcured and compare it with oxygenizing denture cleaner. Aquades is used as a control. The sample consists of 15 acrylic heatcured bars (10x10x2 mm) prepared by contamination on Candida albicans suspensions at temperature 37°C for 24 hours and immersed on 3 different treatments wich are chitosan 2%, oxygenizing denture cleanser, and aquades as control and then, the solutions are being vibrate and put into sabouraud dextrose agar medium. The colony of Candida albicans counted manually. Result shows, highest mean of Candida albicans colony on aquades group (365,66 CFU/plate), followed by oxygenizing denture cleanser treatment (178,33 CFU/plate). The smallest number colony of Candida albicans are found at chitosan 2% treatment (45,33 CFU/plate). Statistical analysis using ANOVA one way shows F=9.826 and p=0, this means, there is minimal one pair of groups with very significant differential (p<0,01) which are chitosan 2% and oxygenizing denture cleanser compared to aquades as control. The difference between those two treatments also significant. Conclusions of this study is that chitosan 2% solution have a sifnificant effect on prohibiting the growth of Candida albicans colony on acrylic heatcured denture base materials. The effectivity of this solution is significantly higher than oxygenizing denture cleanser treatment.

Pengaruh perendaman plat resin akrilik dalam ekstrak kayu manis (Cinnamomum burmanii) terhadap jumlah blastospora Candida Albicans

e-GIGI ◽  
2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Christian Dama
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin ◽  
T Test ◽  
Control Group ◽  
Dilution Method ◽  
Denture Stomatitis ◽  
Cinnamon Extract ◽  
Acrylic Plate ◽  
Removable Denture ◽  
Significant Difference

Resin akrilik sering digunakan sebagai bahan dari gigi tiruan, khususnya basis gigi tiruan. Adanya rongga-rongga mikro pada akrilik menjadi tempat perlekatan sisa-sisa makanan yang dapat meningkatkan jumlah mikroorganisme dalam rongga mulut, salah satunya ialah jamur Candida albicans. Pertumbuhan yang pesat dari jamur Candida albicans menjadi penyebab utama infeksi pada mukosa rongga mulut pemakai gigi tiruan lepasan akrilik, disebut denture stomatitis. Ekstrak kayu manis yang mengandung minyak atsiri, sinamaldehid, eugenol dan juga senyawa seperti flavonoid, Saponin, serta tanin memiliki efek antifungi. Tujuan penelitian ini untuk mengetahui pengaruh dari ekstrak kayu manis dan konsentrasi ekstrak kayu manis terhadap jumlah blastospora Candida albicans pada plat akrilik sehingga mampu mencegah dan menanggulangi penyakit denture stomatitis. Plat akrilik yang telah terkontaminasi dengan jamur Candida albicans direndam dalam ekstrak kayu manis dengan konsentrasi 20%, 30%, 40%, 50%, dan aquades sebagai kontrol. selanjutnya, plat akrilik di getarkan untuk menjatuhkan Candida albicans dalam tabung reaksi dan dihitung jumlah blastosporanya dengan metode pengenceran menggunakan cairan NaCl dan metode hitung langsung pada mikroskop. Hasil perhitungan statistik dengan uji Independent t-test diketahui terdapat perbedaan yang bermakna antara kelompok ekstrak kayu manis dan kelompok kontrol (p ≤ 0,05). Dari hasil penelitian dapat disimpulkan bahwa ekstrak kayu manis dapat memengaruhi pertumbuhan jumlah blastospora Candida albicans pada plat resin akrilik dan meningkat seiring dengan meningkatnya konsentrasi ekstrak kayu manis (cinnamomum burmanii) yang digunakan.Kata kunci: Resin akrilik, Candida albicans, ekstrak kayu manis.ABSTRACTAcrylic resin is often used as an ingredient of artificial teeth, denture base in particular. The existence of micro cavities in acrylic attachment to place leftover food that can increase the number of microorganisms in the oral cavity, one of which is Candida albicans. The rapid growth of Candida albicans is a main cause of infection in the oral mucosa acrylic removable denture wearers, called denture stomatitis. Cinnamon extract that contains essential oils, sinamaldehid, eugenol and also compounds such as flavonoids, saponins, and tannins have antifungal effects. The purpose of this study to determine the effect of cinnamon extract and cinnamon extract concentrations against Candida albicans blastospora number on acrylic plate so as to prevent and control disease denture stomatitis. Acrylic plate that has been contaminated with Candida albicans soaked in cinnamon extract with a concentration of 20%, 30%, 40%, 50%, and sterile water as a control. furthermore, acrylic plate vibrate to drop Candida albicans in a test tube and counted the number of blastospora by using saline dilution method and direct count method on the microscope. The results of calculations with the statistical test known Independent t-test significant difference between cinnamon extract group and the control group (p ≤ 0,05). From the results of this study concluded that cinnamon extract may affect the growth of Candida albicans blastospora on acrylic resin plate and increases with increasing concentration of the extract of cinnamon (Cinnamomum burmanii) were used.Keywords : Acrylic resin, Candida albicans, cinnamon extract.

scholarly journals Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

2012 ◽  
Vol 23 (5) ◽  
pp. 547-554 ◽  
Author(s):  
Delise Pellizzaro ◽  
Gregory Polyzois ◽  
Ana Lucia Machado ◽  
Eunice Teresinha Giampaolo ◽  
Paula Volpato Sanitá ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Reduction Method ◽  
Acrylic Resin ◽  
Chlorhexidine Gluconate ◽  
Common Disease ◽  
Combined Method ◽  
Denture Stomatitis ◽  
Positive Controls

The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.

scholarly journals Kinetics of antifungal activity of home-generated ozonated Water on Candida albicans

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Amirtaher Mirmortazavi ◽  
Hamidreza Rajati Haghi ◽  
Abdolmajid Fata ◽  
Hossein Zarrinfar ◽  
Hossein Bagheri ◽  
...  
Keyword(s):  
Oral Candidiasis ◽  
Test Group ◽  
Acrylic Resin ◽  
Positive Control ◽  
Negative Control ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Acrylic Resins ◽  
Colony Forming Units ◽  
Ozonated Water

Background and Purpose: Candida-associated denture stomatitis is one of the most common forms of oral candidiasis among denture wearers. Regarding this, the aim of the present study was to evaluate the antifungal effects of home-generated ozonated water on the adhesion of the C. albicans attached to the surface of the denture base acrylic resins. Materials and Methods: For the purpose of the study, different concentrations of C. albicans were added to the tubes containing acrylic resin blocks, and then incubated for 2 h at 35°C. The samples were assigned into three groups, each of which contained 42 samples, including normal saline (NS) solution as the negative control, nystatin (N) solution as the positive control, and ozonated water as the test group. The samples were washed and placed in an ultrasonic bath. Subsequently, the saline solution was cultured on Sabouraud dextrose agar. The concentrations of Candida were evaluated during the contact times. Results: The test group (i.e., ozonated water) with 114 colony-forming units (CFU) showed a significant reduction of Candida colonies, compared to the NS group with 2,172 CFU. The 120- and 1-minute incubation with ozonated water showed the highest and lowest effects on the viability of Candida adhered to the acrylic resin, respectively. Conclusion: Based on the findings, home-generated ozonated water can be applied to remove the Candida attached to the surface of the denture plates. Keywords: Antifungal, Candida, Denture, Ozonated water, Stomatitis

scholarly journals Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 221
Author(s):  
Avi Meirowitz ◽  
Arkadi Rahmanov ◽  
Eti Shlomo ◽  
Helena Zelikman ◽  
Eran Dolev ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Candida Albicans ◽  
3D Printing ◽  
Oral Candidiasis ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Heat Curing ◽  
Vitro Effect

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

scholarly journals Pengaruh Rebusan Minyak Atsiri Daun Sirih Merah (Piper Crocatum) sebagai Bahan Pembersih Gigi Tiruan Resin Akrilik terhadap Kekasaran Permukaan dan Perubahan Warna

2020 ◽  
Vol 17 (2) ◽  
pp. 50
Author(s):  
Rismawati Tri Kalasworojati ◽  
Ady Soesetijo ◽  
R. Rahardyan Parnaadji
Keyword(s):  
Surface Roughness ◽  
Essential Oils ◽  
Test Group ◽  
Acrylic Resin ◽  
The Other ◽  
Group Design ◽  
Betel Leaf ◽  
Acrylic Resins ◽  
Post Test ◽  
Dental Practices

Acrylic resins are frequently used as the basic materials of dentures in daily dental practices. Submersion of dentures in disinfectant is usually done by denture users to prevent plaque accumulation. Red betel leaf decoction and essential oils of red betel leaf 10% and 25% can be used as an alternative natural disinfectant contain polyphenols, because it can inhibit the growth of Candida albicans which is commonly found in prosthesis denture users. On the other hand, the polyphenols of red betle leaves was predicted can affect the roughness and discoloration of acrylic resin. The purpose of this study was to determine the differences in surface roughness and discoloration of heat cured acrylic resin submersed in decoction of red betel leaf and essential oil of red betel leaf 10% and 25%. This study was a laboratory experimental with pre and post test group design research.measuring surface roughness value with surface roughness TR 220 and discoloration value with color reader TCR 200. Conclusion: there is a difference in surface roughness and discoloration of acrylic resin that is submersed in decoction of red betel leaves and essential oils of red betel leaf 10% and 25%.

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