Abstract
BackgroundAs a secure vehicle of probiotics, breast milk can provide babies with the Lactobacillus which can not only colonize and inhibit the pathogenic bacterial infection in infant's intestines. The purpose of this study was to assess the occurrence and probiotic properties of Lactobacillus spp. of maternal breast milk in the Uyghur population, Xinjiang China, by using culture method. Results Based on repetitive genomic fingerprinting PCR (rep-PCR), a total of 198 isolates of L actobacill us from 31 different lactation of breast milk samples of Uighur in kashi region of Xinjiang were classified into 11 genotypes, which were identified as Lactobacillus fermentum (82 isolates, prevalence: 61.3%, mean relative abundance: 45.4%, genotype: 3), Lactobacillus brevis (75, 41.9%, 38.1%, 4), Lactobacillus oris (37, 32.3%, 15.1%, 3) and rare Lactobacillus vaginalis (4, 9.7%, 1.4%, 1). From colostrum to mature milk, the number and species of Lactobacillus showed an uptrend, from mature milk to late milk, a downside was found in Lactobacillus. The relative abundance of L. fermentum decreased throughout lactation, while L . brevis showed an opposite trend. Three isolates were randomly selected for each genotype to estimate antimicrobial activity (33 isolates in total). Among the isolates, 20 isolates exhibited broad antibacterial spectrum with inhibition halos >10 mm against most indicator pathogens, which were selected for assessing probiotic properties. Nineteen isolates showed the resistance to vancomycin and co-trimoxazole, and KM66 ( L . brevis ) was resistant up to 5/8 antibiotics. KM147 ( L. brevis ) and Y3 ( L. fermentum ) strains were selected as potential probiotics by combining lysozyme, acid and bile salt tolerance. Conclusion Species composition and prevalence of Lactobacillus varied with the study subjects throughout lactation phase. The deliberately selected Lactobacillus strains from breast milk may have a great potential as probiotics to inhibit pathogen infection in infants.