scholarly journals Occurrence and probiotic properties of Lactobacillus spp. of maternal breast milk in the Uyghur, Xinjiang China, at different lactation stages

2020 ◽  
Author(s):  
Xiaojuan Li ◽  
Xu Li ◽  
Yan Zhang ◽  
Fengwei Tian ◽  
Yongqing Ni
Keyword(s):  
Breast Milk ◽  
Relative Abundance ◽  
Culture Method ◽  
Lactobacillus Brevis ◽  
Probiotic Properties ◽  
Genotype 3 ◽  
Mature Milk ◽  
Uyghur Population ◽  
Lactation Phase ◽  
Antibacterial Spectrum

Abstract BackgroundAs a secure vehicle of probiotics, breast milk can provide babies with the Lactobacillus which can not only colonize and inhibit the pathogenic bacterial infection in infant's intestines. The purpose of this study was to assess the occurrence and probiotic properties of Lactobacillus spp. of maternal breast milk in the Uyghur population, Xinjiang China, by using culture method. Results Based on repetitive genomic fingerprinting PCR (rep-PCR), a total of 198 isolates of L actobacill us from 31 different lactation of breast milk samples of Uighur in kashi region of Xinjiang were classified into 11 genotypes, which were identified as Lactobacillus fermentum (82 isolates, prevalence: 61.3%, mean relative abundance: 45.4%, genotype: 3), Lactobacillus brevis (75, 41.9%, 38.1%, 4), Lactobacillus oris (37, 32.3%, 15.1%, 3) and rare Lactobacillus vaginalis (4, 9.7%, 1.4%, 1). From colostrum to mature milk, the number and species of Lactobacillus showed an uptrend, from mature milk to late milk, a downside was found in Lactobacillus. The relative abundance of L. fermentum decreased throughout lactation, while L . brevis showed an opposite trend. Three isolates were randomly selected for each genotype to estimate antimicrobial activity (33 isolates in total). Among the isolates, 20 isolates exhibited broad antibacterial spectrum with inhibition halos >10 mm against most indicator pathogens, which were selected for assessing probiotic properties. Nineteen isolates showed the resistance to vancomycin and co-trimoxazole, and KM66 ( L . brevis ) was resistant up to 5/8 antibiotics. KM147 ( L. brevis ) and Y3 ( L. fermentum ) strains were selected as potential probiotics by combining lysozyme, acid and bile salt tolerance. Conclusion Species composition and prevalence of Lactobacillus varied with the study subjects throughout lactation phase. The deliberately selected Lactobacillus strains from breast milk may have a great potential as probiotics to inhibit pathogen infection in infants.

scholarly journals Characterization of a Lactobacillus brevis strain with potential oral probiotic properties

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Fang Fang ◽  
Jie Xu ◽  
Qiaoyu Li ◽  
Xiaoxuan Xia ◽  
Guocheng Du
Keyword(s):  
Lactobacillus Brevis ◽  
Probiotic Properties

Gestational Hypertension and Their Impact on Activin A Concentration of the Human Milk

2021 ◽  
Author(s):  
Chiara Peila ◽  
Elena Spada ◽  
Alessandra Coscia ◽  
Stefano Sottemano ◽  
Giulia Fregnan ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Fixed Effects ◽  
Gestational Hypertension ◽  
Random Effect ◽  
Covariance Structure ◽  
Study Groups ◽  
Activin A ◽  
Mature Milk ◽  
Significant Difference

Abstract Introduction It is known that Preeclampsia affects the lactogenesis, but literature data on the effects of this syndrome on the neurobiomarkers composition and Activin A of Human Milk of the lactating mother are not available. The aim of this study is to investigate the effects of this gestational pathology on Activin A levels, a neurobiomarker known to play an important role in the development and protection of the central nervous system.Methods The women recruited in the study were divided in two different study groups: preeclamptic or healthy women. All the breast milk samples were collected using the same procedure into sterile devices BPA-free. Activin A was quantified using an ELISA test. To investigate the effect of pathology in the Activin a concentration in the 3 phases, mixed linear model with unistructural covariance structure, mother as random effect, and fixed effects were performed.Results Activin A was detected in all samples. There were no significant differences between Preeclamptic mother and Normotensive women. The only significant effect is related to the HM phase: in particular, it is significant the difference between colostrum and mature milk (p<0.01).Conclusion There is not any significant difference in Activin A breast milk composition from hypertensive and normotensive women. This result allows us to affirm that breast milk beneficial properties are maintained even if gestational hypertension occurs.

scholarly journals Lutein concentration in human milk during early lactation and its relationship with dietary lutein intake

2009 ◽  
Vol 12 (10) ◽  
pp. 1878-1884 ◽  
Author(s):  
Hellas Cena ◽  
Anna Maria Castellazzi ◽  
Amedeo Pietri ◽  
Carla Roggi ◽  
Giovanna Turconi
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Maternal Plasma ◽  
Cross Sectional Study ◽  
Early Lactation ◽  
Dietary Recommendations ◽  
Mature Milk ◽  
Cross Sectional ◽  
Pregnancy And Lactation ◽  
Age Range

AbstractObjectiveThe present study aimed to estimate the lutein concentration in human milk during early lactation and its relationship with dietary lutein intake measured through the administration of a short FFQ.DesignA cross-sectional study in which an FFQ was administered twice: on day 3 (T0) and day 30 (T1) postpartum; meanwhile two breast milk samples were collected. Maternal plasma samples were obtained at T0. The comparison of dietary lutein intakes and likewise lutein concentrations in breast milk at T0 and T1 were analysed with Student’sttest. Pearson’s correlation coefficient was used to determine the association between dietary lutein intake and lutein concentration in milk and plasma, respectively, as well as the correlation between breast milk and plasma lutein concentrations at T0.SettingPavia, northern Italy.SubjectsTwenty-one pregnant women, age range 24–42 years, were recruited during their last trimester on a voluntary basis.ResultsBoth breast milk and plasma lutein concentrations were significantly correlated with dietary lutein intake (r= 0·86,P= 0·0001 andr= 0·94,P= 0·0001, respectively). There was a clear significant correlation between milk and plasma lutein concentrations (r= 0·87,P= 0·0001). Mature milk lutein concentration, although significantly reduced at T1 (P< 0·01), maintained a fairly high correlation with dietary lutein intake (r= 0·82,P= 0·0001).ConclusionsEven though milk lutein concentration decreased during early lactation, it remained significantly correlated with daily lutein intake. Therefore, while awaiting further research, dietary recommendations advising intake of fresh fruit and vegetables rich in lutein, throughout the whole duration of pregnancy and lactation, are extremely useful.

Human Milk: Benefits, Composition and Evolution

2021 ◽  
Vol 9 (7) ◽  
Author(s):  
Lourdes Franco ◽  
N Serrano ◽  
MP Terrón ◽  
MA Gómez ◽  
AB Rodríguez
Keyword(s):  
Breast Milk ◽  
Clock Genes ◽  
Postnatal Growth ◽  
Biological Processes ◽  
Mature Milk ◽  
Premature Babies ◽  
Daily Food ◽  
Nutritional Needs ◽  
Growth Restrictions ◽  
Term Births

Breastfeeding provides all the energy that the child needs in the form of nutrients in the first months of life. The components cover the nutritional needs in all stages, including colostrum and final or mature milk. It must also be taken into account that the composition of milk varies from one woman to another, between both breasts, between feedings and in the different stages in the same mother. It can be said that variation is an active mechanism to perfectly adjust to the nutritional and immunological needs of each child. Components of breast milk can exert beneficial non-nutritional functions. Breast milk also has bioactive factors, which affect biological processes and, therefore, have an impact on health. In the nutrition of premature babies, parenteral nutrition is carried out first, which later becomes enteral through different strategies, such as early minimal enteral nutrition. Despite this, they still present postnatal growth restrictions, which is associated with adverse neurocognitive outcomes. Breast milk achieves multiple benefits in both preterm and term births. Digestion and absorption in the stomach and intestines follow circadian rhythms in mammals, and these rhythms are regulated by rhythmically expressed clock genes in the intestine, as well as by daily food intake.

Measurement of Cellular Immune Function of Breast Milk and Health Education of Pregnant and Lying in Women

2021 ◽  
Vol 7 (5) ◽  
pp. 2954-2962
Author(s):  
Shasha Hao ◽  
Xiaorong Wang ◽  
Jing Wang
Keyword(s):  
Breast Milk ◽  
Immune Cells ◽  
Mature Milk ◽  
The Third ◽  
Biological Specificity ◽  
Health Related ◽  
Calcium Magnesium ◽  
Ifn Γ ◽  
Transition Milk ◽  
Cellular Immune

Breast milk is different from any nutritional substitutes. Breast milk has biological specificity. The most irreplaceable nutrient for newborns is breast milk. In order to determine the immunoprotective effect of breast milk on newborns, 30 primiparas were selected to obtain a little milk before feeding in three different periods, which were divided into colostrum, transitional milk and mature milk. The contents of CD3, CD4, CD8, SlgA, IgG and IgM positive cells in nuclear cells were observed by inverted fluorescence microscope. The contents of IL-8, IFN - γ, and potassium, sodium, chlorine, calcium, magnesium and phosphorus in different time periods were compared between six groups. The results showed that the content of IFN - γ cells in colostrum was 1.61 and that of IL-8 cells was 0.83. However, the contents of IFN - γ cells and IL-8 cells in colostrum decreased to 0.31 and 0.36 at the time of transition milk. Therefore, breastfeeding from the third to the fifth day after delivery can give more immune cells to the newborn, which is conducive to the establishment of their own immune system. Therefore, in the teaching of health-related knowledge to pregnant women, we should advocate more maternal breastfeeding newborns, for the health of the newborn.

Fatty acid positional distribution in colostrum and mature milk of women living in Inner Mongolia, North Jiangsu and Guangxi of China

2018 ◽  
Vol 9 (8) ◽  
pp. 4234-4245 ◽  
Author(s):  
Long Deng ◽  
Qian Zou ◽  
Biao Liu ◽  
Wenhui Ye ◽  
Chengfei Zhuo ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Breast Milk ◽  
Acid Composition ◽  
Inner Mongolia ◽  
Positional Distribution ◽  
Mature Milk ◽  
Lactating Women ◽  
Food Records ◽  
Milk Samples

In order to investigate the fatty acid composition and distribution in colostrum and mature milk, breast milk samples and 24 h food records were obtained from 65 lactating women across three regions in China.

scholarly journals Human Milk Oligosaccharide, Phospholipid, and Ganglioside Concentrations in Breast Milk from United Arab Emirates Mothers: Results from the MISC Cohort

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2400 ◽  
Author(s):  
Paul McJarrow ◽  
Hadia Radwan ◽  
Lin Ma ◽  
Alastair K.H. MacGibbon ◽  
Mona Hashim ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
United Arab Emirates ◽  
High Performance ◽  
Post Partum ◽  
Human Breast Milk ◽  
Mature Milk ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Individual

Human milk oligosaccharides (HMOs), phospholipids (PLs), and gangliosides (GAs) are components of human breast milk that play important roles in the development of the rapidly growing infant. The differences in these components in human milk from the United Arab Emirates (UAE) were studied in a cross-sectional trial. High-performance liquid chromatography‒mass spectrometry was used to determine HMO, PL, and GA concentrations in transitional (5–15 days) and mature (at 6 months post-partum) breast milk of mothers of the United Arab Emirates (UAE). The results showed that the average HMO (12 species), PL (7 species), and GA (2 species) concentrations quantified in the UAE mothers’ transitional milk samples were (in mg/L) 8204 ± 2389, 269 ± 89, and 21.18 ± 11.46, respectively, while in mature milk, the respective concentrations were (in mg/L) 3905 ± 1466, 220 ± 85, and 20.18 ± 9.75. The individual HMO concentrations measured in this study were all significantly higher in transitional milk than in mature milk, except for 3 fucosyllactose, which was higher in mature milk. In this study, secretor and non-secretor phenotype mothers showed no significant difference in the total HMO concentration. For the PL and GA components, changes in the individual PL and GA species distribution was observed between transitional milk and mature milk. However, the changes were within the ranges found in human milk from other regions.

Safety and efficacy of human breast milk Lactobacillus fermentum CECT 5716. A mini-review of studies with infant formulae

2015 ◽  
Vol 6 (2) ◽  
pp. 219-224 ◽  
Author(s):  
E. López-Huertas
Keyword(s):  
Breast Milk ◽  
Probiotic Strain ◽  
Lactobacillus Fermentum ◽  
Controlled Trials ◽  
Human Breast Milk ◽  
Probiotic Properties ◽  
Human Breast ◽  
Safety And Efficacy

Human breast milk has been described as a source of lactic acid bacteria. Lactobacillus fermentum CECT5716 is a human breast milk strain whose probiotic properties, safety and efficacy has been demonstrated in vitro and in vivo, including controlled trials with human adults. Since the origin of this probiotic strain is human breast milk, we aimed to investigate the safety and efficacy of an infant and a follow-on formulas supplemented with this strain of L. fermentum. We carried out two randomised controlled trials: one trial with infants of 6-12 months of age (follow-on formula study) and another one with infants from 1 to 5 months of age (infant formula study). The results from the trials showed that the probiotic formulas were safe, well tolerated and might be useful for the prevention of community-acquired infections.

scholarly journals Fecal microbiome profiles of neonatal dairy calves with varying severities of gastrointestinal disease

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262317
Author(s):  
Giovana S. Slanzon ◽  
Benjamin J. Ridenhour ◽  
Dale A. Moore ◽  
William M. Sischo ◽  
Lindsay M. Parrish ◽  
...  
Keyword(s):  
Health Status ◽  
Relative Abundance ◽  
Gastrointestinal Disease ◽  
Bifidobacterium Longum ◽  
Dairy Calves ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Fecal Samples ◽  
Fecal Microbiome ◽  
Microbiome Composition

Gastrointestinal disease (GI) is the most common illness in pre-weaned dairy calves. Studies have associated the fecal microbiome composition with health status, but it remains unclear how the microbiome changes across different levels of GI disease and breeds. Our objective was to associate the clinical symptoms of GI disease with the fecal microbiome. Fecal samples were collected from calves (n = 167) of different breeds (Holstein, Jersey, Jersey-cross and beef-cross) from 4–21 d of age. Daily clinical evaluations assessed health status. Calves with loose or watery feces were diagnosed with diarrhea and classified as bright-sick (BS) or depressed-sick (DS) according to behavior. Calves with normal or semiformed feces and no clinical illness were classified as healthy (H). One hundred and three fecal samples were obtained from consistently healthy calves and 64 samples were from calves with diarrhea (n = 39 BS; n = 25 DS). The V3-V4 region of 16S rRNA gene was sequenced and analyzed. Differences were identified by a linear-mixed effects model with a negative binomial error. DS and Jersey calves had a higher relative abundance of Streptococcus gallolyticus relative to H Holstein calves. In addition, DS calves had a lower relative abundance of Bifidobacterium longum and an enrichment of Escherichia coli. Species of the genus Lactobacillus, such as an unclassified Lactobacillus, Lactobacillus reuteri, and Lactobacillus salivarius were enriched in calves with GI disease. Moreover, we created a model to predict GI disease based on the fecal microbiome composition. The presence of Eggerthella lenta, Bifidobacterium longum, and Collinsella aerofaciens were associated with a healthy clinical outcome. Although lactobacilli are often associated with beneficial probiotic properties, the presence of E. coli and Lactobacillus species had the highest coefficients positively associated with GI disease prediction. Our results indicate that there are differences in the fecal microbiome of calves associated with GI disease severity and breed specificities.

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