Efficiency of inosine glycyl-cysteinyl-glutamate disodium and pyridoxine in acute poisoning by unsymmetrical dimethylhydrazine

Effectiveness of pyridoxine hydrochloride and inosine glycyl-cysteinyl-glutamate dinatrium (molixan) in monotherapy and in combination in acute poisoning by unsymmetrical dimethylhydrazine on survival, blood biochemical indicators and the state of plasma coagulation hemostasis was assessed. Unsymmetrical dimethylhydrazine was administered intraperitoneally at a dose of 115 mg/kg (LD50) once. Drugs under examination were also admistered intraperitoneally: pyridoxine hydrochloride in a dose of 50 mg/kg once, 30 minutes after the administration of unsymmetrical dimethylhydrazine, the inosine glycyl-cysteinyl-glutamatedinatrium (molixan) in a dose of 60 mg/kg seven times: 30 minutes after the administration of unsymmetrical dimethylhydrazine, and then daily for 6 days. Biochemical parameters of blood, plasma coagulation hemostasis, were evaluated 7 days after the administration of unsymmetrical dimethylhydrazine. The use of pyridoxine as a monotherapy and in combination with molixan prevents death of experimental animals and the development of convulsive syndrome. In addition, the combination of molixan with pyridoxine eliminates the development of toxic lesions of liver that is having hepatoprotective effect. The efficiency of molixan as hepatoprotector, apparently, related to the fact that in its structure it is a combined preparation containing the peptide and purine components. The peptide component (glycyl-cysteinyl-glutamate dinatrium) is a pharmacological analogue of oxidized glutathione, purine is represented by inosine. Oxidized glutathione has cytoprotective activity, inhibits the cytolysis of hepatocytes, and reduces the severity of inflammatory process during toxic damage of liver cells by the products of metabolism of xenobiotics. Inosine also has hepatoprotective properties. As a precursor to the synthesis of adenosine triphosphoric acid and nucleotides, it stimulates a number of metabolic processes in body, supports energy balance in various tissues, particularly in liver and myocardium.

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Effect of pig housing system on blood biochemical indicators’ level

Journal of Animal Science Biology and Bioeconomy ◽

10.24326/jasbbx.2017.4.5 ◽

2018 ◽

Vol 35 (4) ◽

pp. 45-51

Author(s):

Maria Tietze ◽

Wanda Krupa ◽

Monika Budzyńska ◽

Jarosław Kamieniak ◽

Leszek Sołtys ◽

...

Keyword(s):

Housing System ◽

Biochemical Indicators ◽

Blood Biochemical ◽

Blood Biochemical Indicators

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Comprehensive assessment of the health and physical development in martial arts athletes

E3S Web of Conferences ◽

10.1051/e3sconf/202021017002 ◽

2020 ◽

Vol 210 ◽

pp. 17002

Author(s):

Elena Katamanova ◽

Larisa Vasilieva ◽

Natalia Kuptsova ◽

Irina Kodinets ◽

Irina Kudaeva ◽

...

Keyword(s):

Physical Performance ◽

Recovery Period ◽

Load Test ◽

Step Test ◽

Software Complex ◽

Blood Biochemical ◽

Abdominal Organs ◽

Medium Level ◽

Blood Biochemical Indicators ◽

High Level

Thirty three athletes’ health (average age – 16.4±1.0 years) were studied. Anthropometry and analysis of body composition, and load test were performed by the Hardware-software complex "Zdorovye-Express" (Russia). Spirometry, electrocardiography, assessment of physical performance as per Harvard step test, and ultrasound scan of abdominal organs, blood biochemical indicators were also performed. It is established, examined athletes have medium level of harmony of development and high level of physical fitness. Harvard step test data showed that the vast majority of athletes have an adequate level of physical performance (66.6%) with good recovery ability, and 33.4% - a very poor level of physical performance with a normal or slow recovery period after exercise. Assessing the cardiac singular index, the condition of the cardiovascular system in 82.6% surveyed was considered as optimal and in 17.3% examined as moderately increased. Spirometer indicators confirmed to the age norms. Ultrasound data of the abdominal organs showed the presence of disturbances in 24% of cases, while these disorders were manifested by diffuse liver echogenicity changes in 7% of cases and pathology of the gallbladder in 17% cases. The revealed changes in the health status of athletes require further research.

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Effect of Various Feed Phosphates on Biochemical Indices of Blood and Mineral Composition of Bones in Finishing Pigs

Acta Veterinaria Brno ◽

10.2754/avb201079030355 ◽

2010 ◽

Vol 79 (3) ◽

pp. 355-361 ◽

Cited By ~ 1

Author(s):

Zbigniew Dobrzański ◽

Krystyna Pogoda-Sewerniak ◽

Szymon Dragan ◽

Daniel Korniewicz ◽

Krystyna Hoffmann ◽

...

Keyword(s):

Lactic Dehydrogenase ◽

Low Density Lipoproteins ◽

High Density Lipoproteins ◽

Low Density ◽

Finishing Pigs ◽

Biochemical Indicators ◽

Blood Biochemical ◽

Mineral Components ◽

Blood Biochemical Indicators ◽

Biochemical Indices

The aim of this study was to evaluate the effect of three different chemical feed phosphates on the blood biochemical indicators and the content of main minerals of bones in finishing pigs. Over a period of 85 days of fattening, monocalcium (MCP, Finnish product), dicalcium (DCP, Polish product) and calcium-sodium (CSP, Russian product) phosphates were used in fattener feeding. The feeding was based on standard mixtures of starter, grower and finisher type. Dicalcium phosphate was produced according to the new, pro-ecological technology based on phosphoric acid. The content of Ca, Na, P, solubility of P in citric acid, and the concentration of undesirable substances (As, Cd, F, Hg and Pb) were determined in feed phosphates. At the end of the fattening period, blood was collected from 36 finishing pigs (12 from each group) and the following biochemical indicators were determined in the serum: enzymatic activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyltransferase (GGT), creatine kinase (CK), lactic dehydrogenase (LDH), lactic acid (LA); the concentration of total protein, albumins, glucose, urea, creatinine, content of triglycerides, cholesterol and its high density lipoproteins (HDL) and low density lipoproteins (LDL) fractions, and mineral components concentration (Ca, Cl, Cu, Fe, K, Mg, Na, P, Zn). Basic macroelement content (Ca, Mg, P) was determined in the thigh bones from 30 pigs (10 from each group). Significant differences (p < 0.05) between groups were observed only in some biochemical indicators, i.e. CK, LDH and LA. The highest content of Ca, Mg and P was found in the bones of pigs fed mixtures supplemented with DCP which indicates improved bioavailability of main macroelements from that phosphate.

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Genetic parameters for the prediction of abdominal fat traits using blood biochemical indicators in broilers

British Poultry Science ◽

10.1080/00071668.2017.1379052 ◽

2017 ◽

Vol 59 (1) ◽

pp. 28-33 ◽

Cited By ~ 1

Author(s):

H. L. Zhang ◽

Z. Q. Xu ◽

L. L. Yang ◽

Y. X. Wang ◽

Y. M. Li ◽

...

Keyword(s):

Genetic Parameters ◽

Abdominal Fat ◽

Biochemical Indicators ◽

Blood Biochemical ◽

Blood Biochemical Indicators

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Comparison of the Metabolites of Water Polo Players before and after Competition by the Metabolomic Approach

Journal of Healthcare Engineering ◽

10.1155/2021/7600835 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Jingjing Wang ◽

Mohammed Abdella Kemal

Keyword(s):

Ketone Bodies ◽

Urine Ph ◽

Blood Samples ◽

Biochemical Indicators ◽

Water Polo ◽

Metabolic Characteristics ◽

Blood Biochemical ◽

Before And After ◽

Blood Biochemical Indicators ◽

Metabolomic Approach

Background. The metabolic characteristics of body fluids of excellent water polo players before and after competition have not been reported. The purpose of the study was to compare the metabolites in the urine of water polo players before and after competition by 1H-NMR-based metabolomic approach. Methods. Twenty-six young water polo players participated in the study voluntarily. The urine and blood samples of players were collected one week before competition (A), immediately after competition (B), and one week after competition (C). Metabolomic analysis was conducted on the urine samples. Urine routine items and biochemical indicators in blood samples were detected. Results. Metabolomic results showed that the contents of eleven metabolites including lactic acid, acetoacetate, and succinic acid in the urine of the subjects increased and four metabolites such as dimethylamine, choline, and glucose decreased at stage B. Most metabolites at stage C had basically returned to the levels at stage A. Pyruvate metabolism, pantothenate and CoA biosynthesis, synthesis, and degradation of ketone bodies were mainly involved in the above process. Urine conventional analysis results showed that the urine pH decreased dramatically and the levels of PRO and URO significantly increased at stage B, and the three indicators had similar values between stages A and C. The other indicators did not have obvious difference among the three stages. Analysis of blood biochemical indicators showed that the levels of LDH, BUN, CK, and AST significantly increased at stage B and did not show an obvious difference between stages A and C. The results are helpful for coaches to arrange the athletes’ diet reasonably and to conduct scientific training for athletes.

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The relationship between urinary Alzheimer-associated neuronal thread protein and blood biochemical indicators in the general population

Aging ◽

10.18632/aging.103356 ◽

2020 ◽

Vol 12 (15) ◽

pp. 15260-15280

Author(s):

Yuxia Li ◽

Shaochen Guan ◽

He Jin ◽

Hongjun Liu ◽

Meimei Kang ◽

...

Keyword(s):

General Population ◽

Biochemical Indicators ◽

Blood Biochemical ◽

Blood Biochemical Indicators ◽

The Relationship

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Systematic Safety Evaluation of Cold Plasma-Activated Liquid in Rabbits

Frontiers in Physics ◽

10.3389/fphy.2021.659227 ◽

2021 ◽

Vol 9 ◽

Author(s):

Yujing Xu ◽

Sansan Peng ◽

Bing Li ◽

Shuai Wang ◽

Hao Zhang ◽

...

Keyword(s):

Bone Marrow ◽

Body Weight ◽

New Zealand ◽

Safety Evaluation ◽

Toxicity Tests ◽

Biochemical Indicators ◽

Blood Biochemical ◽

Acute Toxicity Tests ◽

Blood Biochemical Indicators ◽

New Zealand Rabbits

Plasma-activated liquid (PAL) can effectively and selectively kill various types of cancer cells both in superficial and deeper tumors. As a promising novel approach to oncotherapy, the safety of PAL is essential in the clinic but has not been thoroughly assessed. In myeloma and blood tumors, the pathogenesis is in the bone marrow cavity. We have therefore evaluated the safety of PAL in New Zealand rabbits by intra-bone marrow injection, and provide a basis of further clinical research and application of PALs. In this study, both a plasma jet and plasma surface were used to treat saline solution, phosphate-buffered solution, and cell culture medium, to produce PAL. Then, oscillograms and optical emission spectra were evaluated to characterize the plasma discharge. Acute toxicity tests and safety evaluation studies were conducted by intra-bone marrow injection of PAL into New Zealand rabbits, while control rabbits received saline only. Body weight, vital organ coefficient, organ appearance, organ histopathology, blood cell and hemoglobin parameters, and blood biochemical indicators were tested on the 30th day after injection. We found that there was no mortality or loss of mobility throughout the experimental period. Acute toxicity tests showed that there were no PAL-related side effects in rabbits receiving the maximum dose of 700 μL PAL. PAL treatments did not affect body weight, organ coefficient, organ appearance, organ histopathology, or blood biochemical indicators. However, the percentage of lymphocytes decreased while the percentage of neutrophil granulocytes increased compared with the control group. In summary, our results indicate that PAL can be safely injected into bone marrow of New Zealand rabbits without significant toxicity.

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METABOLIC STATUS OF THE ORGANISM OF BULLS-PRODUCERS OF DIFFERENT GENOTYPES

Molochnoe i miasnoe skotovodstvo ◽

10.33943/mms.2020.45.31.008 ◽

2020 ◽

Author(s):

Н.В. БОГОЛЮБОВА ◽

Р.А. РЫКОВ

Keyword(s):

Blood Serum ◽

Mineral Metabolism ◽

The Body ◽

Holstein Breed ◽

Blood Biochemical ◽

Health And Nutrition ◽

Biochemical Status ◽

Comparative Aspect ◽

Holstein Bulls ◽

Blood Biochemical Indicators

Изучили биохимический статус организма быков-производителей голштинской (n=8) и симментальской (n=8) пород в сравнительном аспекте. Проанализированы пробы сыворотки крови быков-производителей, содержащихся в условиях племпредприятия Московской области. В образцах крови определяли показатели азотистого, углеводно-липидного, минерального обменов, активность ферментов. Содержание общего белка и его фракций, мочевины, а также соотношение альбуминов и глобулинов были примерно на одном уровне независимо от генотипа животных. В крови быков симментальской породы, по сравнению с аналогами голштинской породы, была выше концентрация креатинина на 19,9% (P<0,05), уровень фосфолипидов — на 16%, холестерин — на 46,5% (P<0,05). Концентрация фосфора в сыворотке крови симментальских быков составляла 2,52 мМ/л, что на 30,5% больше, чем у голштинов (P<0,05). Отношение кальция к фосфору в организме симментальских быков равнялось 1,04 против 1,40 ед. в группе голшинских быков. Полученные данные указывают на различия в протекании азотистого, углеводно-липидного и минерального обменов в организме животных мясо-молочного и молочного направлений продуктивности и помогут в разработке эталонных значений биохимических показателей крови для оценки здоровья и уровня питания быков-производителей изучаемых пород. We studied the biochemical status of the organism of Holstein (n=8) and Simmental (n=8) bulls-producers in a comparative aspect. Samples of blood serum of bulls-producers contained in the conditions of the Moscow region were analyzed. Indicators of nitrogen, carbohydrate-lipid, mineral metabolism, and enzyme activity were determined in the blood samples. The content of total protein and its fractions, urea, as well as the ratio of albumins and globulins were approximately at the same level, regardless of the genotype of animals. In the blood of Simmental bulls, compared with analogues of the Holstein breed, the concentration of creatinine was higher by 19.9% (P<0.05), the level of phospholipids — by 16%, and cholesterol — by 46.5% (P<0.05). The concentration of phosphorus in the blood serum of Simmental bulls was 2.52 mmol/l, which is 30.5% higher than in Holsteins bulls (P<0.05). The ratio of calcium to phosphorus in the body of Simmental bulls was 1.04 against 1.40 units. in the Holstein bulls. The obtained data indicate differences in the nitrogen, carbohydrate-lipid and mineral metabolism in the body of animals of meat-and-milk and dairy productivity and will help in the reference values of blood biochemical indicators development for assessing the health and nutrition level of bulls-producers of the studied breeds.

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Effects of Water Temperature on Biochemical Characteristics of Mandarin Fish during Live Transportation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.941-944.1092 ◽

2014 ◽

Vol 941-944 ◽

pp. 1092-1098

Author(s):

Shou Kun Cheng ◽

Shun Sheng Chen ◽

Li Juan Wang ◽

Shuo Lin Huang

Keyword(s):

Respiratory Rate ◽

Water Temperature ◽

Short Distance ◽

Ammonia Nitrogen ◽

Mandarin Fish ◽

Long Distance ◽

Biochemical Indicators ◽

Blood Biochemical ◽

Opposite Trend ◽

Blood Biochemical Indicators

Objective and Methods: The effect of water temperature on Mandarin fish during live transportation was studied through testing the respiratory rate, ammonia nitrogen in water, glycogen and lactic acid in flesh and blood biochemical indicators at 20°C, 15°C,10°C and 5°C after transported for 0h, 3h, 10h and 24h. Results: The respiratory rate declined with the decreasing temperature and extending transportation time; the ammonia nitrogen in water increased with the temperature falling and lapse of time; the glycogen dropped as time went by and 24h later it declined with the temperature falling; but lactic presented a completely opposite trend; The cortisol decreased with the temperature declined, and rose first and then fell with the time; AST had a negative correlation with water temperature but GLU had a positive correlation; UREA decreased significantly with the declined temperature between 20°C and 10°C, and the level of it at 5°C was close to at 20°C.Conclutions: All these results revealed that 5°C should be selected for short distance transportation less than 10h and 10-15°C was the best choice for long distance transportation.

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