scholarly journals THE ROLE OF SYMPATHIC AND PARASIMPATIC INNERVATION IN NEUROIMMULAR INTERACTIONS WITH EXTERNAL GUINETAL ENDOMETRIOSIS

2019 ◽  
Vol 19 (1S) ◽  
pp. 56-58
Author(s):  
A E Andreev ◽  
A O Drobintseva ◽  
V O Polyakova
Keyword(s):  
Pain Syndrome ◽  
Nerve Fibers ◽  
Diagnostic Methods ◽  
Reproductive Age ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Parasympathetic Nerve ◽  
Pgp 9.5 ◽  
Alexa Fluor

Currently the study of the role of peripheral nervous system in control of inflammation in endometriosis and its involvement in the development of the chronic pain syndrome in this pathology is of relevance. The purpose of this work was to assess the reciprocal location of sympathetic and parasympathetic nerve fibers with external genital endometriosis at various stages of progression. The samples for the study were the endometrial biopsies of patients of reproductive age with external genital endometriosis of I-IV stage of the disease, with primary sterility (n = 20). The control group consisted of 3 patients (n = 5) who did not have any signs of endometriosis. Molecular markers were visualized using the immunohistochemical method. Antibodies to tyrosine hydroxylase (TH, 1:200, Abcam, USA) and PGP 9.5 (PGP 9.5, 1:1000, Abcam, USA) were used as primary antibodies. Alexa Fluor 488 (1:1000, Abcam, USA) and Alexa Fluor 647 (1:1000, Abcam) were used as secondary antibodies. The results were visualized with a confocal microscope (FluoView1000 (Olympus)). 3D shooting was used to assess the reciprocal location of the studied markers. As a result of this work, it was found that the expression of both of studied markers was detected in all samples. The obtained data can significantly increase the understanding of the functioning mechanisms of the neoneurogenesis processes in ectopic and eutopic tissue, thereby allowing an increase in the accuracy of the endometriosis diagnostic methods.

Clinical and sonological features in endometriomas in women of reproductive age

2016 ◽  
pp. 85-89
Author(s):  
О. Shapoval ◽  
Keyword(s):  
Endometrial Hyperplasia ◽  
Benign Tumor ◽  
Pain Syndrome ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Ovarian Endometriosis ◽  
Gynecological Examination ◽  
Different Characteristics ◽  
Ovarian Endometriomas

The objective: to study the prevalence of ovarian endometriosis in women of reproductive age, the features of clinical and ultrasound picture of endometriosis. Patients and methods. The study involved 22 patients with endometriomas, the control group – 50 women gynecological and somatically healthy. Results. The incidence of ovarian endometriomas in the structure of benign tumor-like formations of ovaries is 0.62%. In 72.73% of ovarian endometriomas occur on a background of concomitant gynecological pathology. Clinically, in 77.27% of cases there is a pain syndrome, in 59.09% – algomenorrhea, in 13.64% – infertility; 18.18% of cases endometriomas remain «dumb» and proceed with the erased clinical picture. Sonologically in patients with endometriomas adenomyosis, endometrial hyperplasia, changes in the contralateral ovary are determined. Conclusion. Without additional methods of diagnostic gynecological examination may identify the 3 cm tumor-like formation of the ovary with different characteristics, which does not allow to differentiate endometrioma from inflammation, functional and ovarian tumors. Ultrasound can diagnose pathological ovarian formation of 1 cm, detailing the nature of the cyst. Key words: endometriosis, reproductive age, retrospective analysis, ultrasound.

scholarly journals Research Progress on the Effect of Sphingosine Kinase 2 on Glioma

2021 ◽  
Vol 5 (6) ◽  
pp. 16-19
Author(s):  
Chunpeng Wang ◽  
Xiaohui Liu ◽  
Shiwen Guo
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Sphingosine Kinase ◽  
Immunohistochemical Method ◽  
Research Progress ◽  
Control Group ◽  
Sphingosine Kinase 2 ◽  
Two Factors ◽  
The Central Nervous System

Objective: The purpose of this study is to explore the role of sphingosine kinase 2 (SphK2) in the treatment of glioma, which is the most common primary tumor in the central nervous system. Methods: A total of 82 patients were included in this study, with 27 cases in the control group and 55 cases in the glioma group; the expressions of SphK2 and gp130 in the two groups were compared by immunohistochemical method, and the correlation between the two factors was analyzed. Results: Both SphK2 and gp130 were upregulated in the glioma group, and the two factors were significantly correlated. Conclusion: The high expression of SphK2 may play an important role in the occurrence, development, and diagnosis of glioma.

scholarly journals Genital endometriosis and melatonin: a role in the pathogenesis and its possible use in the treatment of the disease

2019 ◽  
Vol 68 (3) ◽  
pp. 51-60 ◽  
Author(s):  
Maria I. Yarmolinskaya ◽  
Saimat Sh. Tkhazaplizheva ◽  
Arseniy S. Molotkov ◽  
Natalia N. Tkachenko ◽  
Valentina L. Borodina ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Melatonin Receptors ◽  
Reproductive Age ◽  
Control Group ◽  
Eutopic Endometrium ◽  
Relative Expression ◽  
Treatment Regimens ◽  
Daily Urine ◽  
New Treatment

Hypothesis/aims of study. Melatonin is found in almost all living organisms, the range of its effects being quite diverse. Effects of this hormone in the human body are realized via two ways, through specific receptors and non-receptor pathways. Melatonin may act through both membrane and nuclear receptors. In the present work, the expression of MTR1 and MTR2 melatonin receptors was studied in the eutopic endometrium and endometrioid heterotopies, and the level of melatonin metabolite, 6-sulfatoxymelatonin, in daily urine in patients with genital endometriosis (GE) was analyzed. Study design, materials and methods. The experimental group included 67 patients of reproductive age with a verified diagnosis of GE, and the control group consisted of 18 individuals with an ovulatory menstrual cycle without gynecological pathology. The 6-sulfatoxymelatonin level in daily urine was determined by enzyme immunoassay. The study of MTR1 and MTR2 melatonin receptor expression in the endometrium and endometrioid heterotopies was performed in 24 patients with GE and in 10 women of reproductive age who were examined for infertility who did not have gynecological pathology based on diagnostic laparoscopy. To study the expression of melatonin receptors, the endometrium and endometrial heterotopy sampling was carried out from day 18 to day 22 of the menstrual cycle. Morphological assessment included histological and immunofluorescence studies using confocal laser scanning microscopy. Results. In patients with GE, there was found a tendency to a decrease in 6-sulfatoxymelatonin excretion in daily urine compared to the control group. It was also found that the total relative expression area of melatonin receptors in the endometrium of women with GE was significantly lower compared to the endometrium of patients from the control group. Significant differences between the average brightness and optical density were not found. In addition, it was revealed that the relative expression areas of MTR1 and MTR2 melatonin receptors in the eutopic endometrium and in endometrioid heterotopies did not differ significantly. A negative correlation was stated between the relative expression area of melatonin receptors and GE prevalence. Particular attention is paid to the role of melatonin in the development of GE and to the possibilities of working out new treatment regimens with its use. Conclusion. The data obtained confirm the undoubted role of melatonin in the pathogenesis of GE, however, the development of new treatment regimens with its use requires further study.

Evaluation the Role of Ghrelin and Leptin as Biochemical Marker in Female with Polycystic Ovarian Syndrome

2021 ◽  
Vol 20 ◽  
Author(s):  
Mahmod Khalil Ibrahim ◽  
Amina Hamed Ahmed Alobaidi
Keyword(s):  
Metabolic Syndrome ◽  
Biochemical Marker ◽  
Polycystic Ovary ◽  
Sandwich Elisa ◽  
Reproductive Age ◽  
Control Group ◽  
Ovary Syndrome ◽  
Serum Leptin ◽  
Serum Ghrelin

Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. PCOS is a heterogeneous complex disorder of unwell defined aetiology. Some studies report its association to various endocrine, metabolic and immunological abnormalities. The hunger hormones ghrelin and leptin affect the pathogenesis of PCOS and might lead to the development of metabolic syndrome (MS) in obese women. Aim: The study aims at evaluating the role of ghrelin and leptin level in female with poly cystic ovary syndrome as a biochemical marker for the diagnosis and monitoring progression. Subjects and Methods: The study including one hundred PCOS patients and fifty apparently healthy subjects with regular menstrual cycle, visiting gynecology outpatient clinic of Kalar General Hospital, from the beginning of February 2015 to the end of June 2015. Body mass index (BMI) along with serum ghrelin, leptin, Luteinizing hormone (LH), Follicle stimulating hormone (FSH) and testosterone levels were measured for both groups. Serum leptin was determined using sandwich ELISA, while serum ghrelin was determined using competitive ELISA. Differences between patient and control groups were tested by using t-test. Also one way Anova was used to test the relation among different groups. Results: There was a significant decrease in ghrelin level and increase in leptin levels in women with PCOS when compared with control group. Also there was a significant elevation in serum level of LH ,Testosterone , Prolactin and decrease in serum FSH in PCOS patients when compared to control group. Additionally, serum ghrelin decreased and serum leptin level increased significantly in women with PCOS compared to controls in all age and BMI groups. Conclusion: The significant decrease in ghrelin and increased in leptin in PCOS patients than in controls indicated that they are at high risk for metabolic syndrome development.

scholarly journals Neurovascular Interrelations and Tissue Microcirculation in Posttraumatic Reflex Sympathetic Dystrophy

2004 ◽  
Vol 11 (2) ◽  
pp. 74-77
Author(s):  
A I Krupatkin ◽  
M A Berglezov ◽  
V A Kolosov ◽  
A I Krupatkin ◽  
M A Berglezov ◽  
...  
Keyword(s):  
Reflex Sympathetic Dystrophy ◽  
Sympathetic Neurons ◽  
Objective Assessment ◽  
Treatment Protocol ◽  
Clinical Importance ◽  
Pain Syndrome ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Vasomotor Function

Thirty eight patients with posttraumatic reflex sympathetic dystrophy (RSD) of upper extremities and 28 healthy individuals of the same age (control group) were examined using computer laser doppler flowmetry (LDF) and wavelet-analysis of amplitude-frequency spectrum of fluctuations of microvascular circulation. RDS prevailed in patients with primary warm forms, increase ofportion of shunt circulation, decrease of sympathetic activity and its breakdown at activation. Patients with primary cold forms were characterized by inconstant increase of neurogenic tonus of microvessels at rest. Prolong activation of peptidergenic function of sensory fibres supporting neurogenic inflammation, edema and pain syndrome were typical. One could not exclude that sympathetic vasomotor function and sympathetic support of pain may innervated by different groups of sympathetic neurons. Objective assessment of perivascular innervation and tissue microcirculation is of great clinical importance for individual RSD treatment protocol. Use of LDF is reasonable for early diagnosis of RSD, determination the role of sympathetic and peptidergenic mechanisms in RSD pathogenesis and indications for sympathetic blockades and capsaicin.

scholarly journals Osteoporosis and pain severity in reproductive age patients with breast cancer

2016 ◽  
Vol 97 (2) ◽  
pp. 207-212
Author(s):  
N Y Tretyakova ◽  
A V Chizhikov
Keyword(s):  
Breast Cancer ◽  
Combined Treatment ◽  
Pain Syndrome ◽  
Reproductive Age ◽  
Pain Severity ◽  
Control Group ◽  
Mcgill Pain Questionnaire ◽  
Complex Treatment ◽  
Mineral Density ◽  
Osteoporosis Diagnosis

Aim. To analyze the relationship of osteoporosis in reproductive age patients with breast cancer with the pain severity and osteoporosis diagnosis improvement in these patients to improve their quality of life.Methods. The study included 63 women aged 30 to 50 years. The studied group consisted of 33 women with confirmed diagnosis of breast cancer, control group - 30 healthy, same age women of comparison group. The examination included a blood test for osteocalcin, calcitonin, alkaline phosphatase, parathyroid hormone, thyroid stimulating hormone, calcium and phosphorus. The pain severity was evaluated by McGill pain questionnaire and pain syndrome classification. Osteodensitometry was performed to all patients of studied and control groups. All patients with «breast cancer» diagnosis were examined prior to specific treatment administration and 6 months after treatment course completion. 12 patients were examined also after neoadjuvant chemotherapy.Results.Osteopenia (initial changes of bone tissue) was revealed in 3 (10%) cases in the control group. There was no cases of osteoporosis in the control group. Clinical manifestations in the form of the 1st level pain syndrome in healthy women was registered in 6 (20%) cases. Osteoporosis develops more often in patients who underwent complex treatment with ovarian function blocking, i.e. the artificial menopause advent. Osteoporosis in breast cancer patients after combined treatment was detected in 69.7% of cases, and pain - in 75.8% of cases. Bone mineral density indicators during treatment and after it were significantly different compared to those before treatment (pConclusion. In breast cancer in reproductive age patients after the complex treatment, osteoporosis and pain often develop, therefore such patients need to undergo osteodensitometry for timely osteoporosis diagnosis and treatment correction.

scholarly journals Clinical efficacy feasibility of osteopathic correction for patients with primary algomenorrhea

2019 ◽  
pp. 64-70
Author(s):  
M. N. Urvant ◽  
D. S. Lebedev
Keyword(s):  
Autonomic Dysfunction ◽  
Treatment Effectiveness ◽  
Pain Syndrome ◽  
Reproductive Age ◽  
Control Group ◽  
Menstrual Pain ◽  
Before And After ◽  
Osteopathic Treatment ◽  
And Control ◽  
Experimental Group

Introduction. From 30 to 92 % of reproductive age women suffer from menstrual pain (algomenorrhea). The effectiveness of drug treatment of this suffering does not exceed 50–70 %. Moreover, it is temporary and accompanied by a high incidence of side effects. Dysfunction of the autonomic nervous system is the basis of the algomenorrhea pathogenesis; it is resulting from chronic exposure to psychogenic factor and adaptive mechanisms decrease in thalamus and hypothalamus which is leading to the nociceptive system excitation. Currently, there are scientifi c studies showing the effectiveness of osteopathic correction of autonomic dysfunction that can eliminate menstrual pain.Goal of research — to justify the osteopathic treatment effectiveness for patients with primary algomenorrhea.Materials and methods. The study included 30 women aged from 15 to 29 years who applied to the gynecological offi ce, diagnosed with primary algomenorrhea and a disease period of 3 to 16 years. They were randomly divided into 2 groups: experimental and control, of 15 people each. The patients of experimental group received osteopathic treatment, the patients of control group received drug therapy only. Osteopathic status was determined in all patients before and after treatment. Pain intensity was assessed by using a visual analogue scale (VAS), the direction of autonomic dysfunction — by using the Kerdo index and the severity of autonomic dysfunction — according to the Wayne questionnaire.Results. Dura mater RBN S (93 % of patients) and RBN in the visceral chest region (70 %) were specifi c regional somatic dysfunctions. The remaining regional somatic dysfunctions were common less than in 50 % of patients. Specifi c local somatic dysfunctions (LD) in patients with algomenorrhea were uterus LD (73 %) and mediastinum LD (70 %). Due to osteopathic treatment, the number of regional and local somatic dysfunctions was decreased in the experimental group. Autonomic dysfunction severity and pain syndrome intensity were decreased as well.Conclusion. The results of the study allow us to recommend osteopathic treatment for patients with primary algomenorrhea.

scholarly journals Pathological changes of the cervical spine in children with cervical pain syndrome

2016 ◽  
Vol 4 (4) ◽  
pp. 12-20
Author(s):  
Nellya K. Bakhteeva ◽  
Tat'yana A. Ionova ◽  
Valeriy N. Belonogov ◽  
Sergej P. Bazhanov ◽  
Vladimir V. Ostrovskij
Keyword(s):  
Cervical Spine ◽  
Pain Syndrome ◽  
Diagnostic Methods ◽  
Control Group ◽  
Study Cohort ◽  
Healthy Children ◽  
Cervical Pain ◽  
Pathological Changes ◽  
Older Children ◽  
Basilar Arteries

Background. Interpretation of cervical pain syndrome in children is complicated, resulting in delayed diagnosis of developmental juvenile osteochondrosis. Thus, updating the diagnostic methods of this pathology is particularly important.Aim. To improve methods of cervical spine diagnostics in children with cervical pain syndrome at the base of vertebral and basilar arteries using duplex ultrasound.Material and methods. The study cohort included 148 pediatric patients aged 4–18 years who were divided into two groups: a treatment group of 108 children with cervical pain syndrome and a control group of 40 healthy children. Clinical, radiological (ultrasound, X-ray, and MRI), and statistical methods were used for comparisons.Results. Duplex ultrasound of 108 patients revealed pathological changes of qualitative and quantitative features of C- or S-shaped, corner bend, mesh, excessive, and wave-shaped tortuosity deformities, as well as a reduction or expanse in diameter of one or two of the spinal arteries (SAs). The absence of an influence of osseous cervical spine structures on SAs was considered a sign of congenital genesis of SA deformity, while segmental instability of C2-C3 and/or C3-C4, atlanto-axial subluxation, and a Kimmerle anomaly were considered signs of extravascular compression of SAs. Regardless of the deformity genesis, blood flow was deficit in the vertebral basilar basin because of local hemodynamic disorders at the site of the deformity, particularly in older children. MRI revealed signs of intervertebral disc hypohydration at C2-C3 and/or C3-C4.Conclusion. Pathological changes in SAs of both congenital and acquired genesis resulted in hemodynamic disorders in the vertebral basilar basin in children with cervical pain syndrome, particularly older children.

scholarly journals Ovarian aromatase activity in endometriosis

2013 ◽  
Vol 62 (2) ◽  
pp. 17-22 ◽  
Author(s):  
Valentina Mikhaylovna Denisova ◽  
Vladimir Vsevolodovich Potin ◽  
Mariya Igorevna Yarmolinskaya ◽  
Yekaterina Mikhaylovna Timofeyeva
Keyword(s):  
Antral Follicle ◽  
Reproductive Age ◽  
Control Group ◽  
Aromatase Activity ◽  
Women Of Reproductive Age ◽  
Antral Follicles ◽  
Gonadotropin Releasing Hormone Agonist ◽  
Healthy Women ◽  
Key Enzyme

Aromatase is the key enzyme, which converts androgens into estrogens. To study the role of aromatase in pathogenesis of endometriosis 57 patients and 15 healthy women of reproductive age were examined. Aromatase activity was detected by reaction of estrogens on aromatase inhibitor letrozol intake. Ovarian source of detected aromatase activity was proved by suppression of reaction on letrozole intake during therapy with gonadotropin- releasing hormone agonist. Aromatase activity in recalculation on antral follicle in endometriosis patients withII–IV stages was higher than in healthy women, though total ovarian aromatase activity was not differed from control group because of low number of antral follicles in endometriosis patients. The disturbance of folliculogenesis in endometriosis is connected probably with hyperestronaemia, which has ovarian and extragonandal origin.

Expression of long non-coding RNAs ROR and MALAT1 in uterine fibroids

2021 ◽  
Vol 20 (4) ◽  
pp. 17-21
Author(s):  
S.A. Levakov ◽  
◽  
G.Ya. Azadova ◽  
A.E. Mamedova ◽  
Kh.R. Movtaeva ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Uterine Fibroids ◽  
Reproductive Age ◽  
Expression Level ◽  
Control Group ◽  
Tissue Samples ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Objective. To study the expression level of long non-coding RNAs ROR and MALAT1 in tissue samples of uterine fibroids. Patients and methods. Samples of myomatous nodes and tissues of normal myometrium in 28 women of reproductive age were examined. The analysis of the expression of long non-coding RNAs was carried out using a real-time reverse-transcription polymerase chain reaction (RT-PCR) with specific primers. Results. There was a significant decrease in the expression level of long non-coding RNA ROR and an increase in the MALAT1 expression in tissue samples of uterine fibroids relative to the control group. Conclusion. The results obtained demonstrate a possible role of long non-coding RNAs in the development of uterine fibroids and correlate with the data which we obtained for patients with endometriosis. Detecting the expression level of long non-coding RNAs can improve the existing methods for diagnosing this disease. However, further research is required to determine the clinical significance of MALAT1 and ROR, and the molecular mechanisms underlying the action of these RNAs in uterine fibroid cells. Key words: long non-coding RNAs, uterine fibroids, myomectomy, lncROR, MALAT1

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