scholarly journals Genital endometriosis and melatonin: a role in the pathogenesis and its possible use in the treatment of the disease

2019 ◽  
Vol 68 (3) ◽  
pp. 51-60 ◽  
Author(s):  
Maria I. Yarmolinskaya ◽  
Saimat Sh. Tkhazaplizheva ◽  
Arseniy S. Molotkov ◽  
Natalia N. Tkachenko ◽  
Valentina L. Borodina ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Melatonin Receptors ◽  
Reproductive Age ◽  
Control Group ◽  
Eutopic Endometrium ◽  
Relative Expression ◽  
Treatment Regimens ◽  
Daily Urine ◽  
New Treatment

Hypothesis/aims of study. Melatonin is found in almost all living organisms, the range of its effects being quite diverse. Effects of this hormone in the human body are realized via two ways, through specific receptors and non-receptor pathways. Melatonin may act through both membrane and nuclear receptors. In the present work, the expression of MTR1 and MTR2 melatonin receptors was studied in the eutopic endometrium and endometrioid heterotopies, and the level of melatonin metabolite, 6-sulfatoxymelatonin, in daily urine in patients with genital endometriosis (GE) was analyzed. Study design, materials and methods. The experimental group included 67 patients of reproductive age with a verified diagnosis of GE, and the control group consisted of 18 individuals with an ovulatory menstrual cycle without gynecological pathology. The 6-sulfatoxymelatonin level in daily urine was determined by enzyme immunoassay. The study of MTR1 and MTR2 melatonin receptor expression in the endometrium and endometrioid heterotopies was performed in 24 patients with GE and in 10 women of reproductive age who were examined for infertility who did not have gynecological pathology based on diagnostic laparoscopy. To study the expression of melatonin receptors, the endometrium and endometrial heterotopy sampling was carried out from day 18 to day 22 of the menstrual cycle. Morphological assessment included histological and immunofluorescence studies using confocal laser scanning microscopy. Results. In patients with GE, there was found a tendency to a decrease in 6-sulfatoxymelatonin excretion in daily urine compared to the control group. It was also found that the total relative expression area of melatonin receptors in the endometrium of women with GE was significantly lower compared to the endometrium of patients from the control group. Significant differences between the average brightness and optical density were not found. In addition, it was revealed that the relative expression areas of MTR1 and MTR2 melatonin receptors in the eutopic endometrium and in endometrioid heterotopies did not differ significantly. A negative correlation was stated between the relative expression area of melatonin receptors and GE prevalence. Particular attention is paid to the role of melatonin in the development of GE and to the possibilities of working out new treatment regimens with its use. Conclusion. The data obtained confirm the undoubted role of melatonin in the pathogenesis of GE, however, the development of new treatment regimens with its use requires further study.

scholarly journals Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis

2019 ◽  
Vol 20 (13) ◽  
pp. 3372
Author(s):  
Jeong Sook Kim ◽  
Young Sik Choi ◽  
Ji Hyun Park ◽  
Jisun Yun ◽  
Soohyun Kim ◽  
...  
Keyword(s):  
B Cell ◽  
Cellular Proliferation ◽  
Suppressor Gene ◽  
Cellular Migration ◽  
Matrix Metalloproteinase 2 ◽  
Control Group ◽  
Endometrial Stromal Cells ◽  
Eutopic Endometrium ◽  
Ectopic Endometrium

Estrogen affects endometrial cellular proliferation by regulating the expression of the c-myc gene. B-cell translocation gene 1 (BTG1), a translocation partner of the c-myc, is a tumor suppressor gene that promotes apoptosis and negatively regulates cellular proliferation and cell-to-cell adhesion. The aim of this study was to determine the role of BTG1 in the pathogenesis of endometriosis. BTG1 mRNA and protein expression was evaluated in eutopic and ectopic endometrium of 30 patients with endometriosis (endometriosis group), and in eutopic endometrium of 22 patients without endometriosis (control group). The effect of BTG1 downregulation on cellular migration, proliferation, and apoptosis was evaluated using transfection of primarily cultured human endometrial stromal cells (HESCs) with BTG1 siRNA. BTG1 mRNA expression level of eutopic and ectopic endometrium of endometriosis group were significantly lower than that of the eutopic endometrium of the control group. Migration and wound healing assays revealed that BTG1 downregulation resulted in a significant increase in migration potential of HESCs, characterized by increased expression of matrix metalloproteinase 2 (MMP2) and MMP9. Downregulation of BTG1 in HESCs significantly reduced Caspase 3 expression, indicating a decrease in apoptotic potential. In conclusion, our data suggest that downregulation of BTG1 plays an important role in the pathogenesis of endometriosis.

Evaluation the Role of Ghrelin and Leptin as Biochemical Marker in Female with Polycystic Ovarian Syndrome

2021 ◽  
Vol 20 ◽  
Author(s):  
Mahmod Khalil Ibrahim ◽  
Amina Hamed Ahmed Alobaidi
Keyword(s):  
Metabolic Syndrome ◽  
Biochemical Marker ◽  
Polycystic Ovary ◽  
Sandwich Elisa ◽  
Reproductive Age ◽  
Control Group ◽  
Ovary Syndrome ◽  
Serum Leptin ◽  
Serum Ghrelin

Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. PCOS is a heterogeneous complex disorder of unwell defined aetiology. Some studies report its association to various endocrine, metabolic and immunological abnormalities. The hunger hormones ghrelin and leptin affect the pathogenesis of PCOS and might lead to the development of metabolic syndrome (MS) in obese women. Aim: The study aims at evaluating the role of ghrelin and leptin level in female with poly cystic ovary syndrome as a biochemical marker for the diagnosis and monitoring progression. Subjects and Methods: The study including one hundred PCOS patients and fifty apparently healthy subjects with regular menstrual cycle, visiting gynecology outpatient clinic of Kalar General Hospital, from the beginning of February 2015 to the end of June 2015. Body mass index (BMI) along with serum ghrelin, leptin, Luteinizing hormone (LH), Follicle stimulating hormone (FSH) and testosterone levels were measured for both groups. Serum leptin was determined using sandwich ELISA, while serum ghrelin was determined using competitive ELISA. Differences between patient and control groups were tested by using t-test. Also one way Anova was used to test the relation among different groups. Results: There was a significant decrease in ghrelin level and increase in leptin levels in women with PCOS when compared with control group. Also there was a significant elevation in serum level of LH ,Testosterone , Prolactin and decrease in serum FSH in PCOS patients when compared to control group. Additionally, serum ghrelin decreased and serum leptin level increased significantly in women with PCOS compared to controls in all age and BMI groups. Conclusion: The significant decrease in ghrelin and increased in leptin in PCOS patients than in controls indicated that they are at high risk for metabolic syndrome development.

Modern approaches to the treatment of bacterial vaginosis and mixed nonspecific vaginitis

2017 ◽  
pp. 89-95
Author(s):  
O.V. Golyanovsky ◽  
◽  
V.V. Mehedko ◽  
M.A. Budchenko ◽  
◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Standard Treatment ◽  
Main Group ◽  
Reproductive Age ◽  
Control Group ◽  
Complete Disappearance ◽  
Complete Elimination ◽  
Women Of Reproductive Age ◽  
High Effectiveness ◽  
Treatment Regimens

The objective: to evaluate the effectiveness of treatment of women with various dysbiotic vaginal conditions with Limenda. Materials and methods. 58 women of reproductive age with BV and mixed nonspecific vaginitis participated in the study. The main group included 30 women, whose treatment was carried out with Limenda drug. The control group consisted of 28 women, whose treatment was performed according to the standard scheme using a combination of drugs. Results. Based on the results of control tests, complete elimination of pathogens occurred in 29 (96.67%) patients of the main group and in 26 (92.86%) patients of the control group. Five days after the treatment, all 30 (100%) women in the main group noted complete disappearance of symptoms on the control visit, and 2 (7.14%) of the control group complained of minor discomfort in the vagina, which required continued treatment. The conclusion. For the treatment of nonspecific vaginitis and bacterial vaginosis, especially chronic and often recurrent forms, it is better to use combined local remedies. The results of this study reliably testify to the high effectiveness of the combined drug Limenda compared with standard treatment regimens. Key words: vaginitis, bacterial vaginosis, vaginal biocenosis, conditional resistance, metronidazole, miconazole, Limenda.

Potential Effect of β-casomorphin-7 on Lymphocyte and TLR\NF-κB Signaling Pathway in Intestinal Mucosa of Aged Mice

2020 ◽  
Author(s):  
Hong Yin ◽  
Xiaqing Su ◽  
Jijie Liu ◽  
Lihua Li ◽  
Lihua Li ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Intestinal Mucosa ◽  
The Elderly ◽  
Potential Effect ◽  
Protective Role ◽  
Control Group ◽  
Relative Expression ◽  
Aged Mice ◽  
Histopathological Studies

The effect of â-Casomorphin-7 on intestinal mucosal immunity was investigated in aged mice. Mice were treated without or with different doses of â-Casomorphin-7 for 30 days. All mice were sacrificed and intestinal mucosa samples collection at the end of the experiment. Histopathological studies showed the tissue protective role of â-Casomorphin-7 in aged mice. The number of duodenal and jejunal epithelial lymphocytes decreased significantly Pandlt;0.05 The doses of duodenal and jejunal epithelial lymphocytes in mice were significantly Pandlt;0.05 increased in each dose group. The relative expression of TLR4,TRAF6 and NF-êB in the intestinal mucosa of the elderly model group was lower than that of the young control group. The low and middle dose groups significantly Pandlt;0.05 up-regulated the relative expression of TLR4, TRAF6 and NF-êB.The results suggest that â-Casomorphin-7 can improved intestinal mucosal immune decline likely through balancing TLR4\NF-êB signaling pathway.

Nocturnal Melatonin Excretion is Decreased in Patients With Migraine Without Aura Attacks Associated With Menses

Cephalalgia ◽  
1995 ◽  
Vol 15 (2) ◽  
pp. 136-139 ◽  
Author(s):  
J Brun ◽  
B Claustrat ◽  
P Saddier ◽  
G Chazot
Keyword(s):  
Menstrual Cycle ◽  
Pineal Gland ◽  
Environmental Conditions ◽  
Luteal Phase ◽  
Migraine Without Aura ◽  
Biological Rhythms ◽  
Control Group ◽  
Migraine Group ◽  
The Mean

Nocturnal melatonin excretion was studied throughout a complete menstrual cycle in 10 women with migraine without aura attacks associated with menses and 9 women controls. Urine melatonin was determined by radioimmunoassay. The mean nocturnal melatonin excretion throughout the cycle was significantly lower in the migraine patients than in controls. In the control group, melatonin excretion increased significantly from the follicular to the luteal phase, whereas no difference was observed in the migraine group. Results are discussed in view of the role of the pineal gland in the organization of biological rhythms and homeostasis in relation to environmental conditions.

scholarly journals The role of melatonin in polycystic ovary syndrome: A review

2019 ◽  
Author(s):  
Sina Mojaverrostami ◽  
Narjes Asghari ◽  
Mahsa Khamisabadi ◽  
Heidar Heidari Khoei
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Free Radical Scavenger ◽  
Melatonin Receptors ◽  
Reproductive Age ◽  
Radical Scavenger ◽  
Ovary Syndrome ◽  
Follicular Maturation

Background: Polycystic ovary syndrome (PCOS) is a widespread endocrine disorder, affecting approximately 20% of women within reproductive age. It is associated with hyperandrogenism, obesity, menstrual irregularity, and anovulatory infertility. Melatonin is the main pineal gland hormone involved in the regulation of the circadian rhythm. In recent years, it has been observed that a reduction in melatonin levels of follicular fluid exists in PCOS patients. Melatonin receptors in the ovary and intra-follicular fluid adjust sex steroid secretion at different phases of ovarian follicular maturation. Moreover, melatonin is a strong antioxidant and an effective free radical scavenger, which protects ovarian follicles during follicular maturation. Objective: In this paper, we conducted a literature review and the summary of the current research on the role of melatonin in PCOS. Materials and Methods: Electronic databases including PubMed/MEDLINE, Web of Science, Scopus, and Reaxys were searched from their inception to October 2018 using the keywords ″Melatonin″ AND ″Polycystic ovary syndrome" OR "PCOS.″ Results: Based on the data included in our review, it was found that the administration of melatonin can improve the oocyte and embryo quality in PCOS patients. It may also have beneficial effects in correcting the hormonal alterations in PCOS patients. Conclusion: Since metabolic dysfunction is the major finding contributing to the initiation of PCOS, melatonin can hinder this process via its improving effects on metabolic functions. Key words: Hyperandrogenism, Infertility, Melatonin, PCOS.

scholarly journals The Effects of Classical Music based Chakra Meditation on the Symptoms of Premenstrual Syndrome

2015 ◽  
Vol 2 (3) ◽  
Author(s):  
Poornima Viswanathan ◽  
Nishal Pinto ◽  
Poornima Viswanathan
Keyword(s):  
Menstrual Cycle ◽  
Premenstrual Syndrome ◽  
Classical Music ◽  
Intervention Group ◽  
Symptom Clusters ◽  
Reproductive Age ◽  
Control Group ◽  
Post Test ◽  
And Control ◽  
Experimental Group

Premenstrual syndrome (PMS) is a very common condition, occurring in women of reproductive age. This is an important source of stress for women, significantly affecting their regular functioning. Previous studies have shown that alternative forms of therapy like meditation have an effect on the symptoms associated with PMS. However, the direct link between the two has not been focused on by the larger group of researchers. In this study, the researcher intended to study the effects of classical music based chakra meditation on the symptoms of premenstrual syndrome. The sample of the study comprised of 40 college students who were screened for premenstrual syndrome based on the Shortened Premenstrual Assessment Form (SPAF) and Calendar of Premenstrual Experiences(COPE) which they were expected to fill in for one menstrual cycle. The participants who qualified for the study were allotted to the intervention group and control group. The intervention group was expected to practice a 15 minute music based chakra meditation, everyday, for one menstrual cycle, during which they also filled in the COPE. The control group was expected to fill the COPE for one menstrual cycle. Both the groups filled in the SPAF after the completion of the menstrual cycle. The results on the SPAF and COPE showed that there was significant reduction in all the post-test symptom clusters of the experimental group, compared with the pre-test symptoms. Thus, this study demonstrates that music based chakra meditation can have significant healing effects on the symptoms of premenstrual syndrome.

scholarly journals Ovarian aromatase activity in endometriosis

2013 ◽  
Vol 62 (2) ◽  
pp. 17-22 ◽  
Author(s):  
Valentina Mikhaylovna Denisova ◽  
Vladimir Vsevolodovich Potin ◽  
Mariya Igorevna Yarmolinskaya ◽  
Yekaterina Mikhaylovna Timofeyeva
Keyword(s):  
Antral Follicle ◽  
Reproductive Age ◽  
Control Group ◽  
Aromatase Activity ◽  
Women Of Reproductive Age ◽  
Antral Follicles ◽  
Gonadotropin Releasing Hormone Agonist ◽  
Healthy Women ◽  
Key Enzyme

Aromatase is the key enzyme, which converts androgens into estrogens. To study the role of aromatase in pathogenesis of endometriosis 57 patients and 15 healthy women of reproductive age were examined. Aromatase activity was detected by reaction of estrogens on aromatase inhibitor letrozol intake. Ovarian source of detected aromatase activity was proved by suppression of reaction on letrozole intake during therapy with gonadotropin- releasing hormone agonist. Aromatase activity in recalculation on antral follicle in endometriosis patients withII–IV stages was higher than in healthy women, though total ovarian aromatase activity was not differed from control group because of low number of antral follicles in endometriosis patients. The disturbance of folliculogenesis in endometriosis is connected probably with hyperestronaemia, which has ovarian and extragonandal origin.

scholarly journals Prolactin level correction in restoration of menstrual cycle in obese women

2021 ◽  
pp. 53-58
Author(s):  
T.F. Tatarchuk ◽  
N.V. Kosei ◽  
S.I. Reheda ◽  
M.I. Hlamazda ◽  
I.M. Shakalo
Keyword(s):  
Metabolic Syndrome ◽  
Menstrual Cycle ◽  
Reference Values ◽  
Prolactin Level ◽  
Experimental Studies ◽  
Main Group ◽  
Reproductive Age ◽  
Control Group ◽  
Upper Limit ◽  
Cycle Disorders

Obesity is a common problem among women of reproductive age. There are data from clinical and experimental studies indicating the role of hyperprolactinemia in the development of metabolic syndrome, as well as the metabolic benefits of treating hyperprolactinemia.Research objective: to evaluate the clinical efficacy of Vitex Agnus Castus standardized extract on hormonal and metabolic status in women with menstrual cycle disorders and obesity.Materials and methods. 60 women aged 19 to 35 years with obesity and menstrual cycle disorders with laboratory-confirmed insulin resistance and prolactin near the upper limit of the reference values or was slight increase were examined. All the patients were given recommendations for lifestyle modification. 30 of them, who formed the main group, received Cyclodynon. 30 patients of the control group were not prescribed Cyclodynon therapy.Results. At the end of the treatment, the menstrual cycle was normalized in 93.3% patients of the main group, and in 66.3% patients of the control group (p < 0.05). Ovulation signs were detected in 86.67% women of the main group and only in 30.0% women of the control group (p < 0.05). The mean prolactin value and the HOMA index were significantly lower in the main group; prolactin level did not exceed the physiological values in 86.67% women in the main group after 6 months, and in 40.0% of the control group, p < 0.05. There was a decrease in body mass index and waist/hip circumference ratio in patients of the studied groups; the dynamics was slightly higher in the main group but did not differ significantly. Conclusions. Cyclodynon is effective for the treatment of menstrual cycle disorders and metabolic disorders in women with obesity and metabolic syndrome. Good tolerability, absence of side effects, and sufficient efficacy of Cyclodynon allow it to be recommended as the drug of choice in patients with prolactin levels near the upper limit of the reference values or a slight increase of it and in patients with intolerance to synthetic dopamine agonists.

Expression of long non-coding RNAs ROR and MALAT1 in uterine fibroids

2021 ◽  
Vol 20 (4) ◽  
pp. 17-21
Author(s):  
S.A. Levakov ◽  
◽  
G.Ya. Azadova ◽  
A.E. Mamedova ◽  
Kh.R. Movtaeva ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Uterine Fibroids ◽  
Reproductive Age ◽  
Expression Level ◽  
Control Group ◽  
Tissue Samples ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Objective. To study the expression level of long non-coding RNAs ROR and MALAT1 in tissue samples of uterine fibroids. Patients and methods. Samples of myomatous nodes and tissues of normal myometrium in 28 women of reproductive age were examined. The analysis of the expression of long non-coding RNAs was carried out using a real-time reverse-transcription polymerase chain reaction (RT-PCR) with specific primers. Results. There was a significant decrease in the expression level of long non-coding RNA ROR and an increase in the MALAT1 expression in tissue samples of uterine fibroids relative to the control group. Conclusion. The results obtained demonstrate a possible role of long non-coding RNAs in the development of uterine fibroids and correlate with the data which we obtained for patients with endometriosis. Detecting the expression level of long non-coding RNAs can improve the existing methods for diagnosing this disease. However, further research is required to determine the clinical significance of MALAT1 and ROR, and the molecular mechanisms underlying the action of these RNAs in uterine fibroid cells. Key words: long non-coding RNAs, uterine fibroids, myomectomy, lncROR, MALAT1

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