Antidiabetic, Antibacterial and Antioxidant Potential of Bioactive Peptides Extracted from the Germinated Seeds of Poncirus trifoliata

The seeds proteins are converted into the peptides during the germination through peptidases. These bioactive peptides are fractionated through the enzymes. The bioactive peptides are reported with the number of therapeutic effects like antioxidant, antibacterial, antidiabetic, anti-cancerous and antihypertensive etc. Synthetic drugs have number of adverse effects along with the therapeutic effects, but natural sources are safer and free from this problem. In the present research, Poncirus trifoliata seedling peptides extracts were used to determine antibacterial, antioxidant and anti-diabetic activities. The peptides extract showed substantial growth of inhibition against pathogenic strains including Escherichia coli, Staphylococcus aureus and Bacillus subtilis having the superior effect as compared to amoxicillin antibiotic as a standard drug. The IC50 value of P. trifoliata seeds peptides extract was 32.91 μg mL-1 as compared with standard (IC50) value 25.52 μg mL-1 of ascorbic acid showed good potential as antioxidant activity. The extracted germinated seeds peptides showed considerable antidiabetic activity in controlling the blood glucose level of alloxan induced diabetic rabbits to compare with standard drugs like glibenclamide. Thus, this study confirmed the antibacterial, antioxidant and antidiabetic effects of P. trifoliata seeds peptides is significantly higher as compared to pure drugs. Therefore, further complete separation and detailed investigation study is needed to isolate target peptides and revealed mechanism of action of bioactive peptides and formulation as a drug to replace the synthetics drugs with safe one. © 2021 Friends Science Publishers

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A GREEN AND FACILE APPROACH FOR ANTIDIABETIC AND ANTI-INFLAMMATORY POTENCY FOR FICUS SUBINCISA FRUIT

INDIAN DRUGS ◽

10.53879/id.58.08.12588 ◽

2021 ◽

Vol 58 (08) ◽

pp. 68-74

Author(s):

Abha Shukla ◽

Priyanka Pokhriyal ◽

Rishi K. Shukla ◽

Amanpreet Kaur ◽

Keyword(s):

Ethyl Lactate ◽

Antidiabetic Activity ◽

Green Solvents ◽

Standard Drug ◽

Diet Induced Obesity ◽

Ic50 Value ◽

Health Promoting ◽

Anti Inflammatory ◽

Alcohol Ethyl

Ficus subincisa belongs to the Moraceae family comprising approximately 850 species. Many Ficus species have been used ethnopharmacologically for the treatment of many health-promoting effects. With increasing economical and ecological concerns for several chemical processes, green chemistry is providing various kinds of “green” solvents that can be a recruit for the extraction and isolation of numerous alleviative and important phytoconstituents from plants. The present study was undertaken to prepare crude extracts of F. subincisa fruits with different polarities of green solvents (d-limonene, isopropyl alcohol, ethyl lactate, and hydroalcohol) by using a modified magnetic stirrer extraction method and assessing in vitro anti-diabetic, anti-inflammatory activities by the spectrophotometric method. Among all, ethyl lactate and hydroalcohol fraction of F. subincisa have shown the highest α-amylase and α-glucosidase enzyme inhibitory activity with an IC50 value of 166.91±2.73 and 118.73±0.67 µg/mL, respectively, which were comparable with that of acarbose. At a concentration of 1000 µg/mL, the hydroalcohol and ethyl lactate of fruit produced 134.53±1.23 and 114.67±4.23 µg/mL inhibition of HRBC hemolysis and bovine serum albumin, respectively, as compared with standard drug aspirin and sodium diclofenac. However, there has been no report on the anti-inflammatory and antidiabetic activity of F. subincisa fruit. Therefore this study was aimed to evaluate the anti-inflammatory and antidiabetic activity of F. subincisa fruit extracts of different green solvents. Our study validated the traditional claim with pharmacological data of the Ficus genus. Taken together, these findings imply that the F. subincisa could be useful therapeutic agents to attenuate muscle insulin resistance due to diet-induced obesity and its associated inflammation.

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SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIDIABETIC ACTIVITY OF BAUHINIA RACEMOSA LAM. LEAVES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.24038 ◽

2018 ◽

Vol 11 (6) ◽

pp. 190

Author(s):

Bhimraj Gawade ◽

Mazahar Farooqui

Keyword(s):

Ethanol Extract ◽

Mass Spectrometry Analysis ◽

Antidiabetic Activity ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Screening ◽

Standard Drug ◽

Spectrometry Analysis ◽

Ic50 Value ◽

Inhibitory Potential

Objective: In this study, the leaves of medicinal plant Bauhinia racemosa Lam. with different pharmacological activities were subjected to phytochemical screening and assessment of their in vitro inhibitory potential with porcine pancreatic α-amylase enzyme to treat and management of diabetes.Methods: Plant leaves were extracted sequentially with ethanol solvent. A modified 3,5-dinitrosalicylic acid method was adopted to screen α-amylase inhibition assay. The ethanol extract was analyzed qualitatively and gas chromatography–mass spectrometry analysis technique for the active phytoconstituents according to the standard protocols.Results: A phytochemical screening of leaves extract reveals the presence of carbohydrate, alkaloids, saponin, glycosides, steroids, tannins, flavonoids, triterpenoid, and phenolic compounds. The ethanol extract reported inhibition of α-amylase enzyme activity at IC50 value 61.72 ± 0.03 μg/mL and acarbose as a standard drug at IC50 value 28.07 ± 0.02 μg/mL.Conclusion: The results of the study indicate that B. racemosa Lam. leaves contain some of bioactive phytochemicals might to be exhibiting in vitro antidiabetic activity, which was leading to decreases the rate of starch digestion.

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EVALUATION OF IN VITRO ANTIDIABETIC AND ANTI-INFLAMMATORY ACTIVITIES OF LEAVES EXTRACT OF BOEHMERIA RUGULOSA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.25906 ◽

2018 ◽

Vol 11 (9) ◽

pp. 200

Author(s):

Abha Shukla ◽

Anchal Choudhary

Keyword(s):

Protein Denaturation ◽

Diclofenac Sodium ◽

Significant Inhibition ◽

Inflammatory Activity ◽

Antidiabetic Activity ◽

Standard Drug ◽

Successive Extraction ◽

Ic50 Value ◽

Anti Inflammatory

Objective: The objective of the study is to evaluate in vitro antidiabetic and anti-inflammatory activity of different extracts of leaves of Boehmeria rugulosa by different methods.Methods: In vitro α-glucose and α-amylase were used for antidiabetic activity and lipoxygenase, and protein denaturation method of inhibition assays was used to measure anti-inflammatory activity. Successive extraction of leaves petroleum ether (PE), chloroform (CH), ethyl acetate (EA), acetone (AC), and ethanol (ETH) was performed, and extracts obtained from the extraction were applicable to these activities.Results: The AC extract of leaves shows significantly in vitro antidiabetic activity, and AC has offered significant result 470.07±0.65 μg/mL in the inhibition of α-glucosidase and also for α-amylase assay 698.15±1.71 μg/mL. Acarbose was used as standard. In lipoxidase method, AC had shown better results and in protein denaturation method EA shown the higher inhibition (78.06±0.5 μg/ml) than the other extracts. The standard drug diclofenac sodium also offered significant inhibition against lipoxidase enzyme method with IC50 value 21.76±1.29 μg/mL.Conclusion: These findings suggest that the AC and EA possess potent antidiabetic and anti-inflammatory activities in vitro conditions.

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GC/MS Profile and In-Vitro α-Glucosidase Inhibitory Activity of Essential Oil of Eucalyptus camaldulensis Dehnh Collected from (Erbil) Iraq

Current Bioactive Compounds ◽

10.2174/1573407216999200723112252 ◽

2020 ◽

Vol 16 ◽

Author(s):

Javed Ahamad ◽

Subasini Uthirapathy

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Eucalyptus Camaldulensis ◽

In Vitro Models ◽

Antidiabetic Activity ◽

Standard Drug ◽

Ic50 Value ◽

Gas Chromatography Mass Spectroscopy ◽

Eucalyptus Camaldulensis Dehnh

Background: Eucalyptus camaldulensis Dehnh abundantly found in Erbil, Iraq and commonly used as antispasmodic and antipyretic remedy for treating respiratory tract diseases. The present study aimed to evaluate the effectiveness of E. camaldulensis in achieving glucose homeostasis through the inhibition of α-glucosidase enzyme using in-vitro model and also determine the chemical composition of essential oil by gas chromatography mass spectroscopy (GC/MS). Methods: The chemical composition of E. camaldulensis essential oil by was determined by GC/MS and its antidiabetic activity was assessed through inhibition of α-glucosidase enzyme in in-vitro models. Results: The essential oil yielded 40chemical constituents amounting to 98.55%. The major constituents of essential oil of E. camaldulensis leaf were 1,8-cineole (28.4%), isocaryophyllene oxide (8.4%), β-ocimene (7.9%), α-farnesene (7.9%), globulol (7.3%), terpinen-4-ol (7.6%), isophytol (3.6%), viridiflorol (3.2%), p-cymen-3-ol (3.1%) and α-bisabolol (1.2%). The essential oil of E. camaldulensis showed concentration dependent inhibition of α-glucosidase enzyme and the inhibition range from 75.38±2.09 to 6.08±1.89% for concentration 100 to 3.125 µg/mL. The α-glucosidase inhibition of E. camaldulensis (IC50 value 16.7±2.61 µg/mL) was found almost comparable with standard drug acarbose (IC50 value 12.04±3.17 µg/mL). Conclusion: The result of this study concluded that E. camaldulensis essential oil having 1,8-cineole, isocaryophyllene oxide and β-ocimene as major constituents, the study findings also confirm the traditional claim of its use in the treatment of diabetes mellitus.

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Therapeutic Perspectives of Food Bioactive Peptides: A Mini Review

Protein and Peptide Letters ◽

10.2174/0929866526666190617092140 ◽

2019 ◽

Vol 26 (9) ◽

pp. 664-675

Author(s):

Sulochana Priya

Keyword(s):

Bioactive Peptides ◽

Marine Organism ◽

Biological Effects ◽

Amide Bond ◽

Therapeutic Effects ◽

Specific Sequence ◽

The Past ◽

Cancer Immunity ◽

Microbial Infections ◽

Diverse Origin

Bioactive peptides are short chain of amino acids (usually 2-20) that are linked by amide bond in a specific sequence which have some biological effects in animals or humans. These can be of diverse origin like plant, animal, fish, microbe, marine organism or even synthetic. They are successfully used in the management of many diseases. In recent years increased attention has been raised for its effects and mechanism of action in various disease conditions like cancer, immunity, cardiovascular disease, hypertension, inflammation, diabetes, microbial infections etc. Bioactive peptides are more bioavailable and less allergenic when compared to total proteins. Food derived bioactive peptides have health benefits and its demand has increased tremendously over the past decade. This review gives a view on last two years research on potential bioactive peptides derived from food which have significant therapeutic effects.

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2,4-Dichloro-5-[(N-aryl/alkyl)sulfamoyl]benzoic Acid Derivatives: In Vitro Antidiabetic Activity, Molecular Modeling and In silico ADMET Screening

Medicinal Chemistry ◽

10.2174/1573406414666180924164327 ◽

2019 ◽

Vol 15 (2) ◽

pp. 186-195 ◽

Cited By ~ 3

Author(s):

Samridhi Thakral ◽

Vikramjeet Singh

Keyword(s):

Molecular Docking ◽

Benzoic Acid ◽

Inhibitory Activity ◽

In Silico ◽

Computational Study ◽

Antidiabetic Activity ◽

Standard Drug ◽

Benzoic Acid Derivatives ◽

In Silico Admet ◽

Inhibitory Potential

Background: Postprandial hyperglycemia can be reduced by inhibiting major carbohydrate hydrolyzing enzymes, such as α-glucosidase and α-amylase which is an effective approach in both preventing and treating diabetes. Objective: The aim of this study was to synthesize a series of 2,4-dichloro-5-[(N-aryl/alkyl)sulfamoyl] benzoic acid derivatives and evaluate α-glucosidase and α-amylase inhibitory activity along with molecular docking and in silico ADMET property analysis. Method: Chlorosulfonation of 2,4-dichloro benzoic acid followed by reaction with corresponding anilines/amines yielded 2,4-dichloro-5-[(N-aryl/alkyl)sulfamoyl]benzoic acid derivatives. For evaluating their antidiabetic potential α-glucosidase and α-amylase inhibitory assays were carried out. In silico molecular docking studies of these compounds were performed with respect to these enzymes and a computational study was also carried out to predict the drug-likeness and ADMET properties of the title compounds. Results: Compound 3c (2,4-dichloro-5-[(2-nitrophenyl)sulfamoyl]benzoic acid) was found to be highly active having 3 fold inhibitory potential against α-amylase and 5 times inhibitory activity against α-glucosidase in comparison to standard drug acarbose. Conclusion: Most of the synthesized compounds were highly potent or equipotent to standard drug acarbose for inhibitory potential against α-glucosidase and α-amylase enzyme and hence this may indicate their antidiabetic activity. The docking study revealed that these compounds interact with active site of enzyme through hydrogen bonding and different pi interactions.

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Antimicrobial, Antioxidant, Anti-Acetylcholinesterase, Antidiabetic, and Pharmacokinetic Properties of Carum carvi L. and Coriandrum sativum L. Essential Oils Alone and in Combination

Molecules ◽

10.3390/molecules26123625 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3625

Author(s):

Hafedh Hajlaoui ◽

Soumaya Arraouadi ◽

Emira Noumi ◽

Kaïss Aouadi ◽

Mohd Adnan ◽

...

Keyword(s):

Essential Oils ◽

Coriandrum Sativum ◽

New Drugs ◽

Antidiabetic Activity ◽

Pharmacokinetic Analysis ◽

Bacterial Strains ◽

Standard Drug ◽

Reference Drug ◽

Antioxidant Power ◽

Carum Carvi

Herbs and spices have been used since antiquity for their nutritional and health properties, as well as in traditional remedies for the prevention and treatment of many diseases. Therefore, this study aims to perform a chemical analysis of both essential oils (EOs) from the seeds of Carum carvi (C. carvi) and Coriandrum sativum (C. sativum) and evaluate their antioxidant, antimicrobial, anti-acetylcholinesterase, and antidiabetic activities alone and in combination. Results showed that the EOs mainly constitute monoterpenes with γ-terpinene (31.03%), β-pinene (18.77%), p-cymene (17.16%), and carvone (12.20%) being the major components present in C. carvi EO and linalool (76.41%), γ-terpinene (5.35%), and α-pinene (4.44%) in C. sativum EO. In comparison to standards, statistical analysis revealed that C. carvi EO showed high and significantly different (p < 0.05) antioxidant activity than C. sativum EO, but lower than the mixture. Moreover, the mixture exhibited two-times greater ferric ion reducing antioxidant power (FRAP) (IC50 = 11.33 ± 1.53 mg/mL) and equipotent chelating power (IC50 = 31.33 ± 0.47 mg/mL) than the corresponding references, and also potent activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 19.00 ± 1.00 mg/mL), β-carotene (IC50 = 11.16 ± 0.84 mg/mL), and superoxide anion (IC50 = 10.33 ± 0.58 mg/mL) assays. Antimicrobial data revealed that single and mixture EOs were active against a panel of pathogenic microorganisms, and the mixture had the ability to kill more bacterial strains than each EO alone. Additionally, the anti-acetylcholinesterase and α-glucosidase inhibitory effect have been studied for the first time, highlighting the high inhibition effect of AChE by C. carvi (IC50 = 0.82 ± 0.05 mg/mL), and especially by C. sativum (IC50 = 0.68 ± 0.03 mg/mL), as well as the mixture (IC50 = 0.63 ± 0.02 mg/mL) compared to the reference drug, which are insignificantly different (p > 0.05). A high and equipotent antidiabetic activity was observed for the mixture (IC50 = 0.75 ± 0.15 mg/mL) when compared to the standard drug, acarbose, which is about nine times higher than each EO alone. Furthermore, pharmacokinetic analysis provides some useful insights into designing new drugs with favorable drug likeness and safety profiles based on a C. carvi and C. sativum EO mixture. In summary, the results of this study revealed that the combination of these EOs may be recommended for further food, therapeutic, and pharmaceutical applications, and can be utilized as medicine to inhibit several diseases.

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Hepatoprotective effects of Sapium sebiferum in paracetamol-induced liver injury

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i2.22576 ◽

2015 ◽

Vol 10 (2) ◽

pp. 393 ◽

Cited By ~ 3

Author(s):

Liaqat Hussain ◽

Muhammad Sajid Hamid Akash ◽

Madeha Tahir ◽

Kanwal Rehman

Keyword(s):

Liver Injury ◽

Serum Levels ◽

Therapeutic Effects ◽

Sapium Sebiferum ◽

Dependent Manner ◽

Protective Effects ◽

Drug Induced ◽

Standard Drug ◽

Hepatoprotective Effects ◽

Dose Dependent

Sapium sebiferum leaves were used to determine its hepatoprotective effects against paracetamol-induced hepatotoxicity in mice. A dose dependent study was conducted using two different doses (200 mg/kg and 400 mg/kg) of the extract of S. sebiferum against toxic effects of paracetamol (500 mg/kg) in experimental animal model. Silymarin (50 mg/kg) was used as standard drug to compare therapeutic effects of S. sebiferum with control and paracetamol-treated groups. Paracetamol significantly increased the serum levels of liver enzyme markers like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, and direct bilirubin. The extract showed protective effects by normalizing the liver enzymes markers in a dose dependent manner. Histopathological results confirmed the hepatoprotective effects of leaves of S. sebiferum. We conclude that leaves of S. sebiferum have strong hepatoprotective effects against paracetamol-induced liver injury and can be used in liver injuries caused by drug-induced toxicity.

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“Pharmaco-chemical Characterization and Evaluation of In Vitro Antioxidant and Antidiabetic Activity of Ethanolic Flower Extract of Clerodendrum paniculatum”

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i3.2688 ◽

2020 ◽

Vol 11 (3) ◽

pp. 4569-4577

Author(s):

Sincy Varghese ◽

Kanakasabapathi Devaki ◽

Poornima Kannappan ◽

Sri Rashmy Madathil

Keyword(s):

Secondary Metabolites ◽

Plant Material ◽

Quantitative Estimation ◽

Chemical Characterization ◽

Antidiabetic Activity ◽

Flower Extract ◽

Ms Analysis ◽

Ic50 Value ◽

Biochemical Features

Quantitative and qualitative analysis of different components and antioxidant and activities of the extract of flower (CPF) were analyzed in . identification of of flower was also identified by GC-MS analysis. assess the biochemical features of CPF. solvent extraction of CPF was performed using solvents in increasing order of polarity (petroleum ether, chloroform, ethyl acetate, ethanol, and water) and solvent with maximum profile was standardized for further analysis. Quantitative analysis of selected secondary metabolites like tannin, , alkaloids, and of the flower extract was done by UV . In antioxidant assays and in efficacy of the flower extract were analyzed by respective in assays. identification of in CPF was identified by using GC-MS analysis revealed secondary metabolites in the extract, and further analysis of the extract was performed. Quantitative estimation revealed an accountable amount of secondary metabolites like (47.66mg/g acid equivalent), (24mg/g equivalent), (41mg/g equivalent), and alkaloids (1.79mg/g of extracted plant material). analysis (GC-MS) also confirmed convincing compounds in the extract. From in antioxidant and assay, the IC50 value of the extract of CPF was measured and compared with standard, and from the results, it was evident that the extract had significant in antioxidant and activity. the above results, it can be confirmed thatCPF has got significant and therapeutically active ingredients, as evident in analysis. This is further supported by considerable antioxidant and properties observed in respective assays.

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Evaluation of antidiabetic activity of fruit of Coriandrum sativum. Linn methanolic extract in Streptozocin induced diabetic wistar Albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20175686 ◽

2017 ◽

Vol 7 (1) ◽

pp. 121

Author(s):

Ahmed S. K. ◽

Chakrapani Cheekavolu ◽

Sampath D. ◽

Sunil M.

Keyword(s):

Research Study ◽

Diabetes Management ◽

Methanolic Extract ◽

Coriandrum Sativum ◽

Antidiabetic Activity ◽

Control Group ◽

Albino Rats ◽

Citrate Buffer ◽

Standard Drug ◽

Wistar Albino Rats

Background: Diabetes prevalence is estimated to increase annually. Numerous people use traditional medicine, such as India also considered as the diabetic capital in the world. Diabetes is a metabolic disorder characterized by disturbances in lipid, carbohydrate and protein metabolism. The present study to evaluate the antidiabetic potential of coriandrum sativum. linn fruits methanolic extract in streptozocin induced diabetic wistar albino rats model.Methods: Diabetes induction in wistar albino rats by administration of streptozocin (50mg/kg, i.p.) in citrate buffer. 30 wistar albino rats were divided into 5 groups (A, B, C, D, E). Group A: served as normal control, whereas Group B: diabetic control, Group C, D methanolic coriandrum sativum Linn. fruits extract (CSFME) at a dose of 100, 200mg/kg orally, Group E was given standard drug Glibenclamide (0.5mg/kg) orally. All groups are administered for the period of 14 consecutive days and blood sugar levels was measured at regular intervals up to end of the study.Results: This present research study confirms that the test drug compound CSFME has sustained oral hypoglycaemic activity and statistically significant (p ≤0.05) and which is comparable with standard drug Glibenclamide.Conclusions: This research study confirms that the CSFME has antidiabetic activity against streptozocin induced wistar diabetic albino rats. It could be a novel antidiabetic agent and also a dietary adjunct in the type 2 diabetes management and its complication. Further studies are necessary required to confirm the antidiabetic activity of individual phytochemical compounds of Coriandrum sativum.

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