scholarly journals THE COMBINATORIAL EFFECTS OF Azadirachta indica LEAF EXTRACTS WITH AMIKACIN AND TETRACYCLINE AGAINST CLINICALLY IMPORTANT BACTERIA

2021 ◽  
Vol 9 (Spl-1- GCSGD_2020) ◽  
pp. S133-S138
Author(s):  
L.A. Sivasamugham ◽  
◽  
K. Tze Sin ◽  
K. Thrumaran ◽  
G. Subramaniam ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Antagonistic Effect ◽  
Resistant Bacteria ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Neem Leaf Extract

Antibiotic-resistance is a major threat in the treatment of diseases caused by resistant bacteria. Combination of plant extracts with antibiotics can serve as an alternative to antibiotics. Azadirachta indica (neem plant) has many antimicrobial properties due to the presence of secondary metabolites such as alkaloids and flavonoids. In this study, the combinatorial effects of neem leaf extracts with amikacin and tetracycline against eight clinically important gram-positive and gram-negative pathogens were investigated using the agar well diffusion assay. Synergistic effect of neem leaf extract and tetracycline was observed against Propionibacterium acnes, Bacillus subtilis, and S. pneumoniae with a significant enlargement (p<0.05) in the diameter of the zone of inhibition. However, the same combination showed insignificant inhibition against S. faecalis, Staphylococcus epidermidis, Enterococcus faecalis, and Staphylococcus aureus. The neem leaf extract-amikacin combination showed insignificant antibacterial activity against Staphylococcus aureus, S. epidermidis, S. pneumoniae, and Pseudomonas aeruginosa. An antagonistic effect was observed when Bacillus subtilis was exposed to the same combination as there was a significant reduction (p<0.05) in the zone of inhibition. This study suggests the potential development of the neem leaf extract-tetracycline combination as an antibacterial agent against P. acnes, B. subtilis, and S. pneumoniae. However, this preliminary data requires further investigation and test on a wider range of clinical isolates to make a more decisive conclusion. The antagonistic effect of the neem leaf extract and amikacin suggests that the individual agents are potent as antibacterial agents than the combination.

scholarly journals Antimicrobial Activity of Azadirachta indica, against Target Pathogens and Its Utility as a Disinfectant and Floor Cleaner

2021 ◽  
Vol 10 (25) ◽  
pp. 1899-1903
Author(s):  
Mohsin Ali Khan ◽  
Shadma Yaqoob ◽  
Sharique Ahmad
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Neem Extract ◽  
Inoculum Preparation ◽  
Neem Leaf Extract ◽  
Agar Well Diffusion Method

BACKGROUND Azadirachta indica, commonly known as neem, neem tree or Indian lilac, Limbo, Nim, Nimba, Medusa and Vempu. It is typically grown in tropical and subtropical regions. Neem belongs to family meliaceae. Neem is a native tree of India. It is also called “village pharmacy of south Asia” (India) because of its enormous medicinal properties and people use it most of the time without knowing its multiple advantages. This study was done to know the efficacy of medicinal plant named Azadirachta indica (Neem) against pathogenic microorganisms and about its utility as disinfectant and floor cleaner. METHODS Agar well diffusion method, Culture plate method, inoculum preparation & its subculture methods were used to determine the antimicrobial activity of the neem leaf extract. Different concentrations of neem leaf extracts in culture plates were used for the study. In this method inhibited zones were measured. RESULTS Neem leaf extracts showed considerable antimicrobial activity against four target pathogens. In agar well diffusion method it showed maximum antimicrobial activity against Enterococcus and Staphylococcus aureus. Growth of inhibition was increased as the concentration of the neem extract in agar media increased and was maximum for Staphylococcus aureus. CONCLUSIONS The extract of A. indica, has antimicrobial activity against target pathogens Enterococcus, Staphylococcus aureus, Pseudomonas, and E.coli. With the old medicinal knowledge on neem, better economic and therapeutic utilization can be done by using modern approaches of drug development. KEY WORDS Neem Leaf extract, Antimicrobial Activity, Agar Well Diffusion Test, Zone of Inhibition

scholarly journals Phytochemical and Antimicrobial Properties of Leaves ofAlchonea Cordifolia

2010 ◽  
Vol 7 (3) ◽  
pp. 1071-1079 ◽  
Author(s):  
N. J. George ◽  
I. B. Obot ◽  
A. N. Ikot ◽  
A. E. Akpan ◽  
N. O. Obi-Egbedi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
Bioactive Substances ◽  
Butanol Fraction ◽  
Zones Of Inhibition

The phytochemistry ofAchornea cordifolialeaf extract using different solvents was studied using standard methods. The effects of the leaf extract on some pathogenic bacteria and fungi were also examined. The Phytochemical screening of the leaves shows the presence of useful ethno-botanical bioactive substances such as tannin, saponin, flavonoid, cardiac glycoside and anthraquinone, while alkaloid, phlobatanin and terpene also tested for were absent. The butanol fraction of the extract gives the highest zone of inhibition (13.0 mm) againstStaphylococcus aureus, Escherichia coliandpseudomonas aeruginosa, which are in parity. The control, gentamycin injection gives 12.5 mm, 11.0 mm and 12.0 mm respective zones of inhibition against theStaphylococcus aureus, Eschariclia coliandPseudomonas aeruginosa. Also, the butanol fraction of the extract shows highest zone of inhibition of 17.0 mm againstCandida albican, while ethanolic extract gives 13.0 mm zone of inhibition againstTrichophyton violaceum.

scholarly journals Phytochemical constituent and antimicrobial properties of guava extracts of east Hararghe of Oromia, Ethiopia

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dereje A. Oncho ◽  
Meseret C. Ejigu ◽  
Olyad E. Urgessa
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Shigella Boydii

Abstract Background There is a need for the screening more effective, affordable and readily available antimicrobial substances from local medicinal plants or herbs as the pathogenic bacteria are developing the resistance to common antibiotics. Guava (Psidium guajava L.) has antimicrobial activities and it is easily accessible to local populace in tropical region. Antimicrobial properties of guava extracts are attributed to the presence of different phytochemical constituents. The fact that phytochemicals’ constituents vary as a result of geographical locations and literatures about Ethiopian guava with respect to phytochemicals’ composition and content, and antimicrobial activities are hardly available, there is a need to analyze phytochemicals and antimicrobial properties of the Ethiopian guava from Oromia Regional State, Babile and Gursum Districts against Salmonella Typhi, Shigella boydii, Staphylococcus aureus and Enterococcus faecalis. Methods Extraction was done by maceration. Qualitative analysis of phytochemicals was carried out using standard protocol and quantitative phytochemical determination was conducted using spectrophotometric and gravimetric methods. Antimicrobial activities were determined by disc diffusion and broth dilution method. Results Qualitative phytochemical analysis revealed the presence of alkaloids, saponin, steroids and tannin, but the absence of flavonoid and phlobatannin in all Babile and Gursum leaf and bark extracts. Gravimetric measurement showed the highest terpenoid content of 105.00 ± 8.66 mg extract/g of powder in Gursum leaf extract. Similarly, spectrophotometric measurement showed the highest total phenol content of 0.205 ± 0.01 mg/g of the extracts as Tannic Acid Equivalent in Gursum leaf extract. Antimicrobial activity tests revealed that Babile leaf extract showed the highest 13.0 ± 6.79 mm zone of inhibition against Shigella boydii compared to that of other guava extracts. Babile leaf extract showed the lowest 2.375 ± 0.177 mg/ml minimum inhibitory concentration against Shigella boydii, while Gursum leaf extract showed the lowest 1.875 ± 0.884 mg/ml MIC against Salmonella Typhi. Phytochemical analysis showed the presence of alkaloids, saponins, steroids and tannins in the all extracts, but the absence of flavonoids and phlobatannins in the all extracts and terpenoids in Babile extracts. The highest content of alkaloid and terpenoid (98.67 ± 14.43, 93.33 ± 8.82 mg extract/g of powder, respectively) were found in Babile leaf and Gursum bark extracts, respectively. Antimicrobial activity tests revealed that Babile leaf extracts showed higher zone of inhibition against all clinical isolates than that of Gursum leaf extracts, but Babile bark extracts showed lower zone of inhibition against all clinical isolates than that of Gursum bark extracts. Babile leaf extracts showed the highest zone of inhibition (13.0 ± 6.79 mm) against S. boydii, and Babile bark extracts showed the lowest Minimum Inhibitory Concentration (1.250 ± 0.001 mg/ml) against S. Typhi. Conclusion Guava extracts from different location could be source of natural antimicrobial agents with different composition and content. In vivo antimicrobial activity, and isolation, identification and synergy of specific active compound that responsible for the antibacterial activity should be evaluated.

In vitro Antimicrobial, Antibiofilm and Antiquorum Sensing Activity of Indian Rhododendron (Melastoma malabathricum) against Clinical Isolates of Escherichia coli and Staphylococcus aureus

2021 ◽  
Author(s):  
Honeysmita Das ◽  
A.K. Samanta ◽  
Sanjeev Kumar ◽  
P. Roychoudhury ◽  
Kalyan Sarma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Clinical Isolates ◽  
Antibiofilm Activity ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
E Coli ◽  
Melastoma Malabathricum

Background: Development and persistence of multidrug resistant (MDR) bacteria is considered to be one of the biggest threats to public health worldwide. Development of new antimicrobial agents and alternatives to the conventional antimicrobial agents to control the menace of AMR is the need of the hour. Plants based products can be effectively explored as potential antimicrobial, antibiofilm and antiquorum sensing agents against major bacterial pathogens of human and animals. This present study was conducted to explore the antimicrobial, antibiofilm and antiquorum sensing activity of aqueous and methanol extracts of leaf, flower, fruit and stem of Melastoma malabathricum against clinical isolates of Staphylococcus aureus and Escherichia coli.Methods: E. coli and S. aureus were isolated and identified from diarrhoeic pigs and poultry and mastitic milk of cattle of Mizoram, respectively. Leaf, flower, fruit and stem of M. malabathricum were collected from Mizoram and extracted by methanol and aqueous solvents. The antimicrobial activity and MIC was determined by using well diffusion method and 96 wells microtiter plate method, respectively. Antibiofilm activity of plant extracts was determined in 96 well tissue culture plate. Antiquorum sensing activity was determined by disc diffusion method.Result: Methanol leaf extract exhibited antimicrobial activity against E. coli but not against S. aureus with 18 mm and 6 mm zone of inhibition at 200 mg/mL and 12.5 mg/mL, respectively. Methanol flower extract showed antimicrobial activity against S. aureus but not against E. coli with 14 mm and 6 mm zone of inhibitions at 200 mg/ml and 12.5 mg/mL, respectively. Similarly, the aqueous leaf extract showed antimicrobial activity against S. aureus but not against E. coli with 12 mm and 6 mm zone of inhibition at 200 mg/mL and 100 mg/mL, respectively. The MIC of M. malabathricum methanol leaf extract against E. coli was 3.125 mg/mL, whereas the MIC value of methanol flower and leaf extracts was 6.25 mg/mL against S. aureus. Antibiofilm activity of M. malabathricum methanol leaf, methanol flower and aqueous leaf extracts was recorded only against S. aureus isolates with maximum inhibition at 0.05 mg/mL concentration. Good antiquorum sensing activities was exhibited by the M. malabathricum methanol leaf, methanol flower and aqueous leaf extracts against S. aureus isolates at 200 mg/mL concentration.

scholarly journals COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITY OF SEAWEED (Sargassum muticm) AND FRESHWATER WEED (Spirodela polyrrhiza)

2019 ◽  
Vol 2 (4) ◽  
pp. 80 ◽  
Author(s):  
Navid Safa Nova ◽  
Md. Aftab Uddin ◽  
Tasnia Ahmed
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ethanol Extract ◽  
Sargassum Muticum ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Spirodela Polyrrhiza

Development of new drugs is needed to resist the situation of diseases caused by drug resistant bacteria for public health safety. Natural resource is a big source to find candidates having antibacterial activity and aquatic weed is such a natural resource possessing such activity. The current study was aimed to determine the effectiveness of sea weed (Sargassum muticum) and fresh water weed/duckweed (Spirodela polyrrhiza) against six bacterial isolates Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas luteola and Bacillus subtilis. The potency of methanol and ethanol extracts of these weeds was compared to determine the best candidate of weeds in inhibiting bacteria. Both agar well diffusion method and micro dilution was done to observe the antibacterial activity. Ethanol extract of Sargassum muticum worked best against Pseudomans aeruginosa (30mm zone of inhibition) and no activity against Bacillus subtilis. Methanol extract of the same Sargassum muticum showed less activity compared to ethanol extract except for Bacillus subtilis where it showed 21mm zone of inhibition. Ethanol and methanol extracts of Spirodela polyrrhiza showed less antibacterial activity against the bacteria compared to Sargassum muticum. They showed no antibacterial activity against Klebsiella pneumonia and Staphylococcus aureus. On average, the extracts impart a significant antibacterial activity against these six bacteria which are resistant to several antibiotics. Even one of them (Escherichia coli) is resistant to 4th generation cephalosporin but still fairly susceptible for extracts. The antibacterial properties of these marine and freshwater weeds can be subjected to develop new therapeutics to inhibit the resistant bacteria.

PENGARUH EKSTRAK DAUN MIMBA TERHADAP PERKEMBANGAN HAMA Callosobruchus chinensis L. (Coleoptera : Bruchidae)

AGRICA ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 32-37
Author(s):  
Yustina M.S.W Puu ◽  
Hildegardis Nalti Nansi
Keyword(s):  
Mung Bean ◽  
Leaf Extract ◽  
Callosobruchus Chinensis ◽  
Spawning Activity ◽  
Green Beans ◽  
Leaf Extracts ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Contact Poison ◽  
Neem Leaf Extract

Callosobruchus Chinensis is a pest that damages mung bean seeds in storage and causes damage to both the quality and quantity of seeds. This study aims to determine the effectiveness of neem leaf extracts in suppressing the development of the Callosobruchus Chinensis pest as one of the postharvest pests in the commodity green beans. This research conducted at the Laboratory of the Faculty of Agriculture, University of Flores, by using a Completely Randomized Design (CRD) with five treatments and three replications. The concentration of neem leaf extract treatment is 0 ml / l, 25 ml / l, 30 ml / l, 35 ml / l, and 40 ml / l. The results showed that the concentration of neem leaf extract 40 ml / l caused mortality of C. Chinensis imago as contact poison by 99% and nerve poison by 47%. While the effectiveness of the limb leaf extract on spawning activity was 86%.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF SWIETENIA MAHAGONI – A HIGHLY MEDICINAL PLANT IN INDIA

2016 ◽  
Vol 9 (6) ◽  
pp. 62
Author(s):  
Dhanapaul Vinoth Kumar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Leaf Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Volatile Oils ◽  
Medicinal Uses ◽  
Antibacterial Study ◽  
Active Constituents ◽  
Swietenia Mahagoni

The present study was designed to evaluate the phytochemical analysis and antibacterial activity of Swietenia mahagoni leaf extract. The leaf extract was subjected to a variety of phytochemical analysis. The qualitative phytochemical tests exhibited the presence of common phytocompounds like alkaloids, tannins, saponins, phlobatannins, flavanoids, terpenoids and volatile oils as major active constituents. The plant (Swietenia mahagoni) leaf has valuable medicinal uses, hence petroleum ether (18.2g) was found to be a good solvent for the phytochemical and antibacterial study. Similarly, the Methanolic leaf extract of Swietenia mahagoni has highest zone of inhibition on Bacillus subtilis.

scholarly journals Phytochemical Screening, Antimicrobial and Antioxidant Activities of Sonchus Oleraceus l (Leaves) Extracts

2020 ◽  
pp. 1-4
Author(s):  
Hatim MY Hamadnalla ◽  
◽  
Mahdi Abd Elmageed Mohammed ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Sonchus Oleraceus

The objective of this study to examine phytochemical analysis of sonchus oleraceus l, (asteraceae) (moliata) extracts which were extracted by four solvents, petroleum ether, chloroform, methanol and distill water and to assess their potential antibacterial against four standard bacteria gram-positive and gramnegative (bacillus subtilis staphylococcus aureu, salmonella typhi, and pscudomona aeruginosa) and antioxidant activities. (moliata) extracts using cold maceration method to extract sonchus oleraceus l, (asteraceae) locally known as (moliata) extracts, where dpph assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that tannins, triterpenes, a sterols, flavonoids and cardiac glycosides compounds were present in the sonchus oleraceus l, (asteraceae) lives (moliata) extracts. The methanol extract of leaves showed high antioxidant potential (77± 0.03 % ) and chloroform showed low antioxidant potential activity (4±0.09) compare with propyl gallate as standard (93± 0.010 %) against dpph. Chloroform extract was effective and give moderate zone of inhibition against staphylococcus aureus. Methanol extract was effective and showed moderate zone of inhibition against staphylococcus aureus, bacillus subtilis, salmonella typhi and pseudomonas aeruginosa.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

scholarly journals Azadirachta indica (Neem) Leaf Extract Effect as an Option of Treatment of Ibuprofen-induced Nephrotoxicity

2020 ◽  
pp. 56-62
Author(s):  
Ifeanacho Ezeteonu Abireh ◽  
Onyinye Mary Ozioko ◽  
Ignatius Ikemefuna Ozor ◽  
Elizabeth Finbarrs- Bello ◽  
Uche Sebastine Ozioko ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Kidney Injury ◽  
Wistar Rat ◽  
Male Wistar Rats ◽  
Oral Ibuprofen ◽  
Group 2 ◽  
Neem Leaf Extract ◽  
Group 1

Aim: This study investigated the curative effect of the aqueous leaf extract of Azadirachta indica on Ibuprofen-induced nephrotoxicity in Wistar rat Study Design: This is an experimental research Place of Research: Department of Anatomy, College of Medicine, Enugu State University of Science and Technology. Methodology: Twenty-four male Wistar rats were divided into 6 groups, with 4 rats in each group. Group 1 was control and received oral normal saline 0.5 ml daily. Group 2-6 had induction of nephrotoxicity using oral Ibuprofen 400 mg/Kg daily for 5 days. Group 3-5 were subsequently treated with gavage Azadirachta indica leaf extract 200 mg/Kg, 400 mg/Kg and 800 mg/Kg, respectively, for 5 days. And Group 6 was treated with oral Vitamin E 1000 iu/kg for 5 days. Results: Ibuprofen induced nephrotoxicity as evidenced by elevation of serum creatinine level in group 2 (1.99 ± 0.83), when compared to 0.48 ± 0.07 obtained in group 1 (control), and Bowman’s capsule enlargement with glomerular degeneration observed in group 2. The serum creatinine levels progressively approached the level of that of the control in groups treated with Azadirachta indica leaf extract, groups 3 (1.69 ± 0.52), 4 (0.69 ± 0.10) and 5 (0.49 ± 0.10). Also, the histoarchitecture progressively normalized to that of control with each increase in dose of the extract. Conclusion: Azadirachta indica (neem) leaf extract administration led to the resolution of Ibuprofen-induced kidney injury in this study. Thus, it can serve as a treatment option for kidney injury resulting from ingestion of Ibuprofen, after the identification of the molecule responsible for this effect.

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