Montelukast effects on lymphocyte in asthmatic children

Asthma is a common disease in children with grief consequences on social and educational performance of sufferer. Montelueukas has been a mainstay drug for asthma attack controller medication. However, its effect on blood is not well defined. The main objective is to determine the effect of the drug on white blood cells. This case control study encompasses 72 children with confirmed asthma, 36 of them received 4mg of monteleukast granule daily for four months, the remaining half did not receive the drug. Complete Blood Count (CBC) was performed at the beginning of the study and then monthly till the end of the study. There was no statistically significant difference in the CBC parameters at beginning and during the first two months (P>0.5) between the two group. There was a trend toward a reduction in the lymphocyte count in the treated group in the third month in comparison to the control one. The last CBC measurement (4 months) showed statistically significant reduction in the lymphocyte count (P<0.01) in the treated group when compared to the control group. In conclusion: Monteleukast negatively influence the number of lymphocytes and caution should be exercised to avoid prescribing the drug in conditions known to reduce lymphocyte counts such as Covid-19 infection.

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Effect of Maternal Preeclampsia on Hematological Profile of Newborns in Qatar

BioMed Research International ◽

10.1155/2020/7953289 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Mohammad A. A. Bayoumi ◽

Abir A. H. Ali ◽

Sara G. Hamad ◽

Alaa A. M. Ali ◽

Einas E. Elmalik ◽

...

Keyword(s):

Logistic Regression ◽

Gestational Age ◽

Complete Blood Count ◽

White Blood Cells ◽

Neonatal Resuscitation ◽

Control Group ◽

Chi Square ◽

Hematological Profile ◽

Significant Difference ◽

Neonatal Thrombocytopenia

Background. Preeclampsia is a major cause of pregnancy-related maternal, fetal, and neonatal morbidities and mortalities. We aimed to review the effect of maternal preeclampsia on the hematological profile of newborns in the Qatari population. Methods. In this case-control study, we reviewed data and complete blood count results of neonates born to Qatari women diagnosed of preeclampsia in 2017 in comparison with data of a control group. Statistical analysis was done using unpaired t-test, chi-square test, and logistic regression analysis. Results. A total of 108 neonates of women with preeclampsia and 103 neonates of healthy normotensive women were recruited. The mean weight, length, head circumference, placental weight, and gestational age were significantly lower (P<0.05) in neonates born to women with preeclampsia. Only 13% of babies born to women with preeclampsia developed neonatal thrombocytopenia which is significantly higher compared to only 2% in the control group (chi-square χ2=9.14; P=0.003). No significant difference (P>0.05) was noted between the two groups regarding the white blood cells (WBC) or the absolute neutrophilic count (ANC). Multivariate logistic regression showed that the gestational age, birth weight, length, and ANC had significant association with preeclampsia (P<0.05). Conclusions. We found that there was a positive association between preeclampsia and neonatal thrombocytopenia in the Qatari population. Prematurity, placenta insufficiency, fetal growth restriction, and need for neonatal resuscitation were significantly higher in babies born to women with preeclampsia. We recommend that hematological parameters of neonates of those women should be properly monitored to reduce the chances of developing complications.

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Comparison of hematological parameters and perinatal outcomes between COVID-19 pregnancies and healthy pregnancy cohort

Journal of Perinatal Medicine ◽

10.1515/jpm-2020-0403 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Esin Merve Erol Koç ◽

Rahime Bedir Fındık ◽

Hatice Akkaya ◽

Işılay Karadağ ◽

Eda Özden Tokalıoğlu ◽

...

Keyword(s):

Complete Blood Count ◽

Perinatal Outcomes ◽

Control Group ◽

Strong Positive Correlation ◽

Monocyte Count ◽

Distribution Width ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio ◽

Significant Difference ◽

Adverse Perinatal Outcomes

AbstractObjectivesTo evaluate the relationship between Coronavirus Disease 2019 (COVID-19) in pregnancy and adverse perinatal outcomes. The secondary aim is to analyze the diagnostic value of hematologic parameters in COVID-19 complicated pregnancies.MethodsThe current study is conducted in a high volume tertiary obstetrics center burdened by COVID-19 pandemics, in Turkey. In this cohort study, perinatal outcomes and complete blood count indices performed at the time of admission of 39 pregnancies (Study group) complicated by COVID-19 were compared with 69 uncomplicated pregnancies (Control group).ResultsThere was no significant difference between the obstetric and neonatal outcomes of pregnancies with COVID-19 compared to data of healthy pregnancies, except the increased C-section rate (p=0.026). Monocyte count, red cell distribution width (RDW), neutrophil/lymphocyte ratio (NLR), and monocyte/lymphocyte ratio (MLR) were significantly increased (p<0.0001, p=0.009, p=0.043, p<0.0001, respectively) whereas the MPV and plateletcrit were significantly decreased (p=0.001, p=0.008) in pregnants with COVID-19. ROC analysis revealed that the optimal cut-off value for MLR was 0.354 which indicated 96.7% specificity and 59.5% sensitivity in diagnosis of pregnant women with COVID-19. A strong positive correlation was found between the MLR and the presence of cough symptom (r=41.4, p=<0.0001).ConclusionsThe study revealed that, pregnancies complicated by COVID-19 is not related with adverse perinatal outcomes. MLR may serve as a supportive diagnostic parameter together with the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) in assessment of COVID-19 in pregnant cohort.

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Inhibition of Leukocyte Entry into the Brain by the Selectin Blocker Fucoidin Decreases Interleukin-1 (IL-1) Levels but Increases IL-8 Levels in Cerebrospinal Fluid during Experimental Pneumococcal Meningitis in Rabbits

Infection and Immunity ◽

10.1128/iai.68.6.3153-3157.2000 ◽

2000 ◽

Vol 68 (6) ◽

pp. 3153-3157 ◽

Cited By ~ 47

Author(s):

Christian Østergaard ◽

Runa Vavia Yieng-Kow ◽

Thomas Benfield ◽

Niels Frimodt-Møller ◽

Frank Espersen ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Pneumococcal Meningitis ◽

Interleukin 1 ◽

Treated Group ◽

Control Group ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Significant Difference ◽

The Brain ◽

Experimental Pneumococcal Meningitis

ABSTRACT The polysaccharide fucoidin is a selectin blocker that inhibits leukocyte recruitment into the cerebrospinal fluid (CSF) during experimental pneumococcal meningitis. In the present study, the effect of fucoidin treatment on the release of the proinflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin-1 (IL-1), and IL-8 into the CSF was investigated. Rabbits (n = 7) were treated intravenously with 10 mg of fucoidin/kg of body weight every second hour starting 4 h after intracisternal inoculation of ∼106 CFU of Streptococcus pneumoniae type 3 (untreated control group, n = 7). CSF samples were obtained every second hour during a 16-h study period. Treatment with fucoidin caused a consistent and significant decrease in CSF IL-1 levels (in picograms per milliliter) between 12 and 16 h (0 versus 170, 0 versus 526, and 60 versus 1,467, respectively;P < 0.02). A less consistent decrease in CSF TNF-α levels was observed in the fucoidin-treated group, but with no significant difference between the two groups (P > 0.05). In contrast, there was no attenuation in CSF IL-8 levels. Indeed, there was a significant increase in CSF IL-8 levels (in picograms per milliliter) in the fucoidin-treated group at 10 and 12 h (921 versus 574 and 1,397 versus 569, respectively;P < 0.09). In conclusion, our results suggest that blood-derived leukocytes mainly are responsible for the release of IL-1 and to some degree TNF-α into the CSF during pneumococcal meningitis, whereas IL-8 may be produced by local cells within the brain.

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Efficacy and Safety of Topical Timolol Eye Drops in the Treatment of Myopic Regression after Laser In Situ Keratomileusis: A Systematic Review and Meta-Analysis

Journal of Ophthalmology ◽

10.1155/2015/985071 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Xiaochen Wang ◽

Guiqiu Zhao ◽

Jing Lin ◽

Nan Jiang ◽

Qian Wang ◽

...

Keyword(s):

Meta Analysis ◽

Treated Group ◽

Laser In Situ Keratomileusis ◽

Control Group ◽

Eye Drops ◽

Efficacy And Safety ◽

China National Knowledge Infrastructure ◽

Knowledge Infrastructure ◽

Significant Difference

Aims. The aim of this study was to assess the efficacy and safety of timolol in the treatment of myopic regression after laser in situ keratomileusis (LASIK).Methods. We searched MEDLINE, CENTRAL, EMBASE, China National Knowledge Infrastructure (CNKI), and Chinese Biological Medicine (CBM) from the inception to July 2015 for relevant randomized controlled trials that examined timolol therapy for myopic regression. The methodological quality of the studies included was assessed using the Revman 5.3 software.Results. We included six clinical trials involving 483 eyes in this review, including 246 eyes in treated group and 237 eyes in controlled group. We observed statistically significant improvements on the postoperative SE in the 3 months. However, the change of CCT was not statistically different between the control group and the experimental group. There were fewer cases of IOP, UDVA, and CDVA in treated group having significant difference from the controlled group.Conclusions. Topical timolol could be an effective treatment for reduction of myopic regression especially the spherical errors after myopic LASIK. Further RCTs with larger sample sizes for these trials are warranted to determine the efficacy and limitation for myopic regression after LASIK.

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ASSESSMENT OF IMMUNOMODULATORY POTENTIAL OF AN AYURVEDIC FORMULATION, NIROCIL SYRUP IN WISTAR RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i10.38826 ◽

2020 ◽

pp. 154-158

Author(s):

DINESH DILIP GHADIGAONKAR ◽

MUKESH B CHAWDA ◽

KAPIL S THAKUR

Keyword(s):

Wistar Rats ◽

Animal Ethics ◽

Complete Blood Count ◽

Treated Group ◽

Control Group ◽

Differential Leukocyte Count ◽

Experimental Animals ◽

Prior Approval ◽

Hemagglutination Titer ◽

Immunomodulatory Potential

Objective: This study aims to assess the immunomodulatory potential of an Ayurvedic formulation, Nirocil syrup, in Wistar rats. Methods: The experiments were conducted on Wistar rats with prior approval from the Institutional Animal Ethics Committee. Nirocil syrup was administered for 6 weeks to experimental animals. Parameters such as hemagglutination titer, histopathology of immunological organs, complete blood count, differential leukocyte count, and immunological paw edema were recorded and compared with controlled (untreated) and becozinc treated groups. Results: Nirocil treated group significantly enhanced the antibody titer in comparison to the control group. The results are supported by the increase in blood lymphocyte count and antigenic stimulation in immunological organs (spleen). Nirocil syrup enhanced antibody formation and suppressed the immunological edema in experimental animals. Conclusions: The study concludes that the Ayurvedic formulation Nirocil syrup has immunopotentiating activity.

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THE IMPACT OF ASTHMAAND ITS MEDICATIONS ON DENTAL CARIES STATUS AND STREPTOCOCCUS MUTANS COUNT IN CHILDREN: A CROSS-SECTIONAL STUDY

10.36106/ijsr/7515919 ◽

2020 ◽

pp. 20-23

Author(s):

Sneha Upadhyay ◽

Jyoti Bhavthankar ◽

Mandakini Mandale ◽

Nivedita Kaorey

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Cross Sectional Study ◽

Control Group ◽

Healthy Children ◽

Sectional Study ◽

Cross Sectional ◽

Asthmatic Children ◽

Significant Difference ◽

The Impact

Background: Asthma and its medications have been linked to oral diseases in asthmatic children. Aim: Assessment of the dental caries status, salivary Streptococcus mutans count and S. mutans colony score in children receiving inhaled anti asthmatic medications and their comparison in healthy children Material and Method: A cross-sectional study was performed on 40 asthmatic children and 40 healthy children in the age group of 6-14 years. DMFT/deft indices were calculated and saliva samples were collected. Diluted saliva was inoculated on MSB agar plates. S. mutans count and colony score were analysed after 24-48 hours of inoculation. Results: Statistically significant difference was observed in the mean DMFT/deft index, salivary S. mutans load and S. mutans colony score in children of the asthmatic group and control group. Conclusion: Prevalence of dental caries and cariogenic bacteria is higher in asthmatic children.

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Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-9154 ◽

2016 ◽

Vol 68 (6) ◽

pp. 1487-1496 ◽

Cited By ~ 1

Author(s):

D.S. Lucas ◽

E.T.F. Siqueira ◽

M.M.H. Haguiwara ◽

S.S. Azevedo ◽

S.E. Yotsuyanagi ◽

...

Keyword(s):

Weight Gain ◽

Meat Quality ◽

Treated Group ◽

Control Group ◽

Active Principle ◽

Carcass Quality ◽

Feed Conversion ◽

Zinc Gluconate ◽

Hormone Levels ◽

Significant Difference

ABSTRACT A non-surgical sterilant (NSS) was used with the objective of assessing the zootechnical performance, carcass and meat quality, and hormone levels. 90 male piglets were selected with birthweights varying between 1.5kg to 2.0kg. The control group was constituted of 45 males castrated by the conventional surgical method, at the seventh day of age, and the treated group was constituted of 45 males castrated with NSS (active principle of zinc gluconate in the concentration of 26.2mg/mL associated to the dimethyl sulfoxide at 0.5%), with the application of the first dose on the seventh day of age and the second dose on the fourteenth day of age. The zootechnical assessments were carried out on the farm and consisted: weight gain in the periods per animal and feed conversion. The slaughter of the animals and the assessment of the carcass and meat quality and hormone levels were carried out in a slaughterhouse and the municipality of Campinas (SP). The study demonstrated that the use of NSS was a viable alternative in relation to the zootechnical performance and the carcass quality. In relation to the meat quality there was no significant difference in the majority of the assessed parameters.

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Hematological parameters of the blood count in a healthy population of pregnant women in the Northwest of Morocco (Tetouan-M'diq-Fnideq provinces)

Pan African Medical Journal ◽

10.11604/pamj.2018.29.205.13043 ◽

2018 ◽

Vol 29 ◽

Cited By ~ 2

Author(s):

Saad Bakrim ◽

Youssef Motiaa ◽

Ali Ouarour ◽

Azlarab Masrar

Keyword(s):

Pregnant Women ◽

Reference Values ◽

Blood Count ◽

Complete Blood Count ◽

Hematological Parameters ◽

Control Group ◽

Mean Corpuscular Hemoglobin ◽

Healthy Population ◽

Corpuscular Hemoglobin ◽

Significant Difference

Introduction: numerous biological parameters are physiologically modified during normal pregnancy, in particular hematology. The knowledge of these modifications of the maternal body by biologists and clinicians allows the screening of possible anomalies. In Morocco, the reference values of the complete blood count test for pregnant woman are missing, as are those specific to different trimesters of pregnancy. The aim of this study is to look for the reference values for healthy pregnant women of the Northwest region of Morocco, to compare them to those of non-pregnant women (control) and to those of the literature. Methods: blood samples were taken voluntarily from 3898 healthy pregnant women from 18 to 46 years old who presented themselves at the center of health Kalaa and at the service of gynecology obstetrics of the Provincial Hospital Center of M'diq (Morocco), for prenatal care. To establish the reference intervals of the CBC for non-pregnant women, a control group was constituted by 7035 healthy women from 18 to 50 years old selected according to the Moroccan law of blood donation. The CBC was measured on a Sysmex KX21N® analyzer. For each sample a systematic blood smear was done to determine the leukocyte differential. Results: a statistically significant difference between the pregnant women and control group was noted (p < 0.05) for all the hematological parameters: red blood cells, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, leukocytes, neutrophils, basophils, eosinophils, lymphocytes, monocytes, platelets and mean platelet volume. So, the comparison of the averages established between the first, second and third trimester of pregnancy showed the existence of a significant variation with regard to all the parameters of the CBC test looked for (p < 0.001). Conclusion: the present study provides additional baseline data for basic hematological parameters in healthy pregnant Moroccan women and concluded that pregnancy in women has the tendency to alter some hematological indices. For these reasons, there is an interest to take these modifications into account for optimal maternal and fetal medical care.

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Overexpressed microRNA-141-3p enhance proliferation via targeting PLAG1 in non-diabetic macrosomia

10.1101/338335 ◽

2018 ◽

Author(s):

Dan Guo ◽

Hua Jiang ◽

Yiqiu Chen ◽

Jing Yang ◽

Ziqiang Fu ◽

...

Keyword(s):

Target Gene ◽

Late Pregnancy ◽

Treated Group ◽

Control Group ◽

Placental Development ◽

Mirna Microarray ◽

Qrt Pcr ◽

Significant Difference ◽

Fetal Birth Weight ◽

Proliferation And Invasion

AbstractSeveral studies have shown microRNAs (miRNAs) could regulate the placental development, yet the role and mechanism of miRNAs in the development of non-diabetic macrosomia (NDFMS) remains unclear. The key miRNA that abnormal expressed in NDFMS placentas was screened out by miRNA microarray and verified using qRT-PCR in 91 subjects. The effects of the key miRNA were verified by proliferation assay and invasion assay in HTR-8/SVneo cell, and also in pregnant C57BL/6J mice. miR-141-3p was determined as the key miRNA with the most significant difference, which could promote the proliferation and invasion by regulating the expression of target gene PLAG1. Overexpression of PLAG1 could reverse the effect of cell proliferation and invasion ability caused by miR-141-3p overexpression. Significant difference in fetal birth weight was observed between the control group and treated group with miR-141-3p agomir in late pregnancy, but not in early pregnancy. This study revealed miR-141-3p could increase the proliferation of placenta to participate in the occurrence and development of NDFMS through regulating PLAG1 expression.

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Neutrophil-to-Lymphocyte, Monocyte-to-Lymphocyte and Platelet-to-Lymphocyte Ratios in Relation to Clinical Parameters and Smoking Status in Patients with Graves’ Orbitopathy—Novel Insight into Old Tests

Journal of Clinical Medicine ◽

10.3390/jcm9103111 ◽

2020 ◽

Vol 9 (10) ◽

pp. 3111

Author(s):

Joanna Szydełko ◽

Michał Litwińczuk ◽

Magdalena Szydełko ◽

Beata Matyjaszek-Matuszek

Keyword(s):

Complete Blood Count ◽

Smoking Status ◽

White Blood Cells ◽

Smoking Behavior ◽

Control Group ◽

Clinical Activity ◽

Clinical Parameters ◽

Monocyte Count ◽

Graves Orbitopathy ◽

Insight Into

Graves’ orbitopathy (GO) is an autoimmune disease with a chronic inflammatory background. Smoking behavior is the main environmental factor responsible for the transition of this major extra thyroidal manifestation of Graves’ disease (GD) from the subclinical to the overt form. Complete blood count-derived parameters are suggested to be novel inflammatory indices. The aim of this retrospective study was to investigate the association between neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), and platelet-to-lymphocyte ratios (PLR) with selected clinical parameters and smoking status in 406 GD patients with (n = 168) and without GO (n = 238). The control group consisted of 100 healthy individuals. The activity of GO was graded according to Clinical Activity Score. Significantly higher white blood cells (WBC), neutrophil, and NLR (p < 0.05) values were observed in GD patients with GO compared with those without GO. PLR values were significantly higher in GO patients than in the controls. WBC (6.81 ± 1.56 vs. 5.70 ± 1.23) and neutrophils (3.89 ± 1.06 vs. 3.15 ± 0.95) count was higher in active GO patients than in those with inactive GO. Positive correlation (p < 0.05) between CAS score and WBC, neutrophil and monocyte count, and NLR was found. Smoking was associated with higher WBC (p = 0.040), neutrophil (p = 0.049), PLR (p = 0.032) values. Multivariate analysis revealed that WBC, NLR may be risk factors for GO development. WBC, neutrophil, NLR and PLR values seem to be useful tools in the assessment of inflammation in GD.

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