scholarly journals Comparative efficacy of haloperidol and placebo for treatment of delirium induced by biperiden and LPS in adult male rats based on their learning style and change in their short-term spatial memory

2021 ◽  
Author(s):  
Abdollah Farhadi Nasab ◽  
Malihe Roozbakhsh ◽  
Naghmeh Bahrami
Keyword(s):  
Adult Male ◽  
Sleep Disturbances ◽  
Hospitalized Patients ◽  
High Rate ◽  
Male Rats ◽  
Psychotic Symptoms ◽  
Control Group ◽  
Psychomotor Activity ◽  
Y Maze ◽  
Significant Difference

Background and Objectives: Delirium refers to acute loss of consciousness and is characterized by confused thinking and impaired orientation. It is a life-threatening, but reversible syndrome manifested by cognitive impairment, abnormal psychomotor activity and sleep disturbances. Due to high prevalence in hospitalized patients and high rate of morbidity and mortality, delirium significantly decreases the prognosis of hospitalized patients. Materials and Methods: This experimental animal study evaluated 54 adult male Wistar rats over 2 months of age that weighed 200 to 230 g. Of all, 24 rats received biperiden (40 mg/kg) while the remaining 24 received 50 µg/kg LPS. Induction of delirium was ensured using a Y-maze after 3 hours. The control group included 6 rats The efficacy of different doses of haloperidol for treatment of delirium was assessed 3 h after injection using the Y-maze . Data were compared using one-way ANOVA followed by Tukey’s post-hoc test via GraphPad Prism. Results: Haloperidol at 0.1, 0.2 and 0.5 mg/kg dosage significantly increased the percentage of spontaneous alternation and improved the memory, consciousness and learning compared with biperiden and LPS groups (P<0.01). No significant difference was noted between the haloperidol groups regarding efficacy (P>0.05). Conclusion: Delirium is characterized by attention deficit, impaired orientation, changes in memory, consciousness, perception and mood, and psychotic symptoms. Detection of the complete spectrum of delirium signs and symptoms in an animal that cannot talk (rat) is obviously difficult .using of Y-maze can facilitate this problem .

scholarly journals Synthesis of Silver Nano-particles by Electrochemical Method and the Effects on the Serum Levels of Thyroid Hormones (T3, T4) in Adult Male Rats

2019 ◽  
Vol 13 (4) ◽  
pp. 21-25
Author(s):  
Zohreh Parang ◽  
◽  
Davood Moghadamnia ◽  
Keyword(s):  
Adult Male ◽  
Electrochemical Method ◽  
Wistar Rats ◽  
Serum Levels ◽  
Nano Particles ◽  
Male Rats ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Mean

Background: Silver nano-particles have anti-fungal properties. In the present study, silver nano-particles were synthesized by electrochemical method and its effects on the serum levels ofT3 and T4 hormones were investigated in adult male rats. Methods: In this experimental study, 28 adult male Wistar rats weighing approximately 180-220g were divided into 4 groups of 7 rats. The control group (no treatment). The experimental groups 1and 2 received silver intraperitoneal nano-particle doses of 25 and 100 mg/kg, respectively, for 14 days. The nano-aprticles had been synthesized at 75 seconds interval. The experimental group 3 received silver nano-particles that were synthesized at 300 seconds interval with an intraperitoneal dose of 25 mg/kg for 14 days. At the end of this period, blood samples were obtained from the rats’ hearts, and the serum levels of T3 and T4 hormones were measured. The results were statistically analyzed using ANOVA and Duncan tests. Results: At the completion of the study, there was no significant difference in the mean body weights in all experimental groups compared to control group. The results showed that the mean serum levels of T3 hormone in the experimental group1 increased significantly relative to the control group. However, there was no significant difference in the mean serum levels of T4 hormone in all experimental groups compared to that in the control group (P < 0.05). Conclusions: Silver nano-particles increased the serum T3 hormone level in male Wistar rats.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Reduced Jumping to Conclusion Bias after Experimentally Induced Enhancement of Subjective Body Boundaries in Psychosis

2021 ◽  
Vol 54 (2) ◽  
pp. 92-97
Author(s):  
Naomi Lyons ◽  
Detlef E. Dietrich ◽  
Johannes Graser ◽  
Georg Juckel ◽  
Christian Koßmann ◽  
...  
Keyword(s):  
Sense Of Self ◽  
Psychotic Symptoms ◽  
Control Group ◽  
Implicit Measure ◽  
Relevant Factor ◽  
The Core ◽  
Significant Difference ◽  
Explicit Measure ◽  
Experimental Group ◽  
Core Features

<b><i>Introduction:</i></b> A disturbed sense of self is frequently discussed as an etiological factor for delusion symptoms in psychosis. Phenomenological approaches to psychopathology posit that lacking the sense that the self is localized within one’s bodily boundaries (disembodiment) is one of the core features of the disturbed self in psychosis. The present study examines this idea by experimentally manipulating the sense of bodily boundaries. <b><i>Methods:</i></b> Seventy-three patients with psychosis were randomly assigned to either a 10-min, guided self-massage in the experimental group (EG) to enhance the sense of bodily boundaries or a control group (CG), which massaged a fabric ring. Effects on an implicit measure (jumping to conclusion bias; JTC) and an explicit measure (Brief State Paranoia Checklist; BSPC) of delusion processes were assessed. The JTC measures the tendency to make a decision with little evidence available, and the BSPC explicitly measures the approval of paranoid beliefs. <b><i>Results:</i></b> Patients in the EG showed a lower JTC (<i>M</i> = 4.11 draws before decision) than the CG (<i>M</i> = 2.43; Cohen’s <i>d</i> = 0.64). No significant difference in the BSPC was observed. <b><i>Discussion/Conclusion:</i></b> Our results indicate that enhancing the sense of body boundaries through a self-massage can reduce an implicit bias associated with delusional ideation and correspondingly support the idea that disembodiment might be a relevant factor in the formation of psychotic symptoms.

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p &lt; 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

Insulin secretion in Walker 256 tumor cachexia

1990 ◽  
Vol 258 (6) ◽  
pp. E1033-E1036 ◽  
Author(s):  
L. C. Fernandes ◽  
U. F. Machado ◽  
C. R. Nogueira ◽  
A. R. Carpinelli ◽  
R. Curi
Keyword(s):  
Insulin Secretion ◽  
Islets Of Langerhans ◽  
Adult Male ◽  
Male Rats ◽  
Control Group ◽  
Tumor Bearing ◽  
Isolated Islets Of Langerhans ◽  
Outflow Rate ◽  
Walker 256 ◽  
Walker 256 Tumor

The effect of cachexia on insulin secretion was examined in adult male rats. Isolated islets of Langerhans from Walker 256 tumor-bearing rats secreted less insulin by glucose stimuli as compared with the control group; this was accompanied by significant change in 45Ca2+ outflow rate. Reduced insulin secretion to glucose stimuli in tumor-bearing rats probably led to low insulinemia (one-third). These findings indicate that reduced insulin secretion is probably an important factor for the development of cachexia in Walker 256 tumor-bearing rats.

Acute and subchronic co-administrations to cadmium, diazinon and selenium induce apparent osteoporotic symptoms in adult male rats

Biologia ◽  
2014 ◽  
Vol 69 (10) ◽  
Author(s):  
Hana Ďúranová ◽  
Monika Martiniaková ◽  
Ivana Boboňová ◽  
Radoslav Omelka ◽  
Robert Stawarz ◽  
...  
Keyword(s):  
Adult Male ◽  
Osteoporotic Fractures ◽  
Bone Diseases ◽  
Male Rats ◽  
Control Group ◽  
Femoral Bone ◽  
Negative Effects ◽  
Young Males ◽  
Male Wistar Rats ◽  
Group B

AbstractCadmium (Cd) and diazinon (DZN) are known to be environmental risk factors for various bone diseases including osteoporosis. Selenium (Se), an essential constituent of many antioxidant enzymes, has in higher concentrations negative effects on the bone. The present study was aimed to investigate possible changes in femoral bone of adult male rats after their acute and subchronic exposures to Cd, DZN and Se. A total of 30 male Wistar rats were randomized into three experimental groups. The rats in the group A (4-months-old) were injected intraperitoneally with a mixture of 2 mg CdCl2 kg−1, 20 mg DZN kg−1 and 2 mg Na2SeO3 kg−1 body weight and killed 36 h after xenobiotics had been injected. In the group B, young males (1-month-old) were administered with a combination of 30 mg CdCl2 L−1, 40 mg DZN L−1 and 5 mg Na2SeO3 L−1 in their drinking water, for 90 days. Ten 4-months-old males without toxicant supplementation served as a control group (C). After treatment period, detailed histological analysis of femoral bone was performed in each group. Our results revealed apparent osteoporotic symptoms (resorption lacunae, osteoporotic fractures) in rats from groups A and B. Moreover, histomorphometrical evaluation showed reduced bone vascularization (constricted primary osteons’ vascular canals and Haversian canals) and weakness mechanical properties of bones (smaller size of the secondary osteons) in these rats in comparison with those of the control group. Our study demonstrates for the first time that acute and subchronic co-administrations to Cd, DZN and Se induce evident manifestation characteristics of osteoporosis in male rats.

Dietary fat and adiposity: a dose-response relationship in adult male rats fed isocalorically

1995 ◽  
Vol 268 (4) ◽  
pp. E546-E550 ◽  
Author(s):  
C. N. Boozer ◽  
G. Schoenbach ◽  
R. L. Atkinson
Keyword(s):  
Body Weight ◽  
Body Fat ◽  
Adult Male ◽  
Dietary Fat ◽  
Male Rats ◽  
Control Group ◽  
Low Fat Diet ◽  
Mesenteric Fat ◽  
Total Body Fat ◽  
Total Body

This study examined the effects of increasing levels of dietary fat fed isocalorically on body weight, body composition, and adipose distribution. Adult male rats were weight matched into four groups. One group that was fed a low-fat diet (12%) served as reference controls. The other three groups were fed diets of 24, 36, or 48% fat in amounts to equal the energy intake of the control group. After 6 wk, body weights of the four groups were not significantly different. Intrascapular brown fat did not differ between groups. Total body fat and adipose depot weights, however, increased in proportion to the level of fat in the diet. Total body fat and retroperitoneal and mesenteric depot weights of the 48% fat group were greater than controls (P < 0.05). Mesenteric fat in this group was also significantly increased over all other groups (P < 0.05). These results show that high-fat diets fed to adult animals cause increased body fat in the absence of significant changes in body weight and that mesenteric fat is increased disproportionately.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

scholarly journals A Comparative Study on the Effects of Using Hydroalcoholic Extracts of Linum Usitatissimum and Rosa Damascena on Liver Function in Adult Male Rats

2020 ◽  
Vol 26 (1) ◽  
pp. 54-67
Author(s):  
Mohammad Mehdi Padam ◽  
◽  
Ameneh Khoshvaghti ◽  
Keyword(s):  
Liver Function ◽  
Total Protein ◽  
Linum Usitatissimum ◽  
Colorimetric Method ◽  
Serum Glucose ◽  
Male Rats ◽  
Control Group ◽  
Rosa Damascena ◽  
Glucose Levels ◽  
Significant Difference

Aims: Damage to liver tissue and its dysfunction is very important and if left untreated, it can cause serious problems and even death. In this study, we aimed to investigate the effects of the hydroalcoholic extracts of Linum usitatissimum and Rosa damascena on liver enzymes, total protein, bilirubin, albumin, and serum glucose levels. Materials and Methods: This is a non-randomized clinical trial conducted on 42 male rats divided into 6 groups; control group (group 1) received only sufficient water and food, groups 1 and 2 received 300 and 500 mg/ kgB.W Linum usitatissimum extract, groups 3 and 4 received 500 and 1000 mg/ kgB.W Rosa damascena, and group 6 received 100 mg/ kgB.W Linum usitatissimum plus 250 mg/ kgB.W Rosa damascena extracts intraperitoneally for 28 days. After the last injection, the rats were weighed and their blood samples were collected. The study parameters were measured using a colorimetric method by a spectrophotometer, and then were analyzed using ANOVA and Tukey’s test in SPSS V. 25 at a significance level of P<0.05. Findings: There was no significant difference between alanine aminotransferase, alkaline phosphatase, total and direct bilirubin levels in the control group in comparison with other groups (P>0.05). In the groups received Rosa damascena extract, there was a significant difference between total protein and albumin levels compared to the control group (P<0.05). Moreover, there was a significant difference between serum glucose and aspartate aminotransferase in the control group compared to other groups (P<0.05). Conclusion: Linum usitatissimum and Rosa damascena have no negative effect on the liver function. The probability of diarrhea occurrence and the possible effects on the total protein and serum albumin after using Rosa damascena, and the effects of different doses of Linum usitatissimum on the glucose levels should be taken into account.

scholarly journals Effect of a hydro-alcoholic extract of Melissa officinalis on passive avoidance learning and memory

2019 ◽  
Vol 8 (2) ◽  
pp. 120-125 ◽  
Author(s):  
Zahra Dehbani ◽  
Alireza Komaki ◽  
Farshid Etaee ◽  
Siamak Shahidi ◽  
Masoumeh Taheri ◽  
...  
Keyword(s):  
Passive Avoidance ◽  
Learning And Memory ◽  
Avoidance Learning ◽  
Saline Group ◽  
Passive Avoidance Learning ◽  
Male Rats ◽  
Control Group ◽  
Alcoholic Extract ◽  
Melissa Officinalis ◽  
Significant Difference

Introduction: Melissa officinalis (MO) or lemon balm is traditionally used as a sedative and anti-spasm herbal medicine. There is also evidence that this plant has effects on learning and memory. This study examined the effect of a hydro-alcoholic extract of MO on passive avoidance learning (PAL) and memory in male rats. Methods: A total of 40 adult male Wistar rats were randomly distributed into four groups (200 to 220 g; n = 10 per group); three dose groups (50, 100, and 200 mg/kg of the hydro-alcoholic extract of MO) and vehicle control (saline) group. Saline or doses of extract were administered daily for 14 days by oral gavage. The rats were trained to enter the shuttle box to record their behavior in the PAL task. A retrieval test was performed 24 hours following training. Results: A significant difference was seen in performance among MO groups and the control. MO administered animals had a decreased number of acquisition trials (P < 0.05). In the retention task, MO administered animals had an increased step-through latency (SLT) (P < 0.01), and a decreased latency in the dark compartment (P < 0.001) compared to the control group. Conclusion: The results of the study show that MO can improve learning and memory in the PAL task. Further investigation is needed to enhance our understanding of the neurobiological mechanisms of the MO extract and its effects on learning and memory.

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