scholarly journals Assessment of the Impact of Betulin on the Immune Status of Cows with Leukemia-Associated Infection

2021 ◽  
Author(s):  
Vasily Vlasenko ◽  
Valentina Pleshakova ◽  
Sayat Bayseitov ◽  
Nadezhda Lescheva
Keyword(s):  
Metabolic Activity ◽  
Immune Status ◽  
Leukemia Virus ◽  
Effective Means ◽  
The Body ◽  
Lymphoid Cells ◽  
Serum Samples ◽  
Nonspecific Resistance ◽  
Serological Studies ◽  
The Impact

This study examined the immunity dynamics of bovine brucellosis-complicated leukemia, as well as the efficacy of treatment with betulin. Blood serum samples were taken from cows with specific brucellosis antibodies and bovine leukemia virus antibodies based on the results of complex diagnostic studies. Animals (n = 5) selected based on serological studies were injected subcutaneously with betulin-PEG three times with an interval of 7 days at a dose of 10 mg/kg. Blood was taken for hematological and immunological studies before the injection, and after the first, second and third injections. It was found that leukemia and brucellosis infections were accompanied by a significant increase in the number of leukocytes and lymphoid cells, as well as strong fluctuations in the leukocyte functional and metabolic activity parameters (from insignificant in some individual animals to significantly increased in others). Immunological restructuring of the body after triplicate administration of betulin-PEG was characterized by the restoration of the number of lymphoid cells to the level of clinically healthy animals, as well as a significant increase in the functional and metabolic activity of the neutrophilic granulocytes, especially their oxygen-dependent systems. Based on the results, the product is recommended as an effective means to restrain the leukemia process that is complicated with associated infection, as well as to use for seronegative animals in order to increase nonspecific resistance to BLV infection. Keywords: leukemia, brucellosis, cattle, immune status, betulin, immunocorrection, associated infection

scholarly journals Prophylactic "Xymedon" in Abdominal Surgery

2021 ◽  
Vol 14 (2) ◽  
pp. 152-160
Author(s):  
Nikita A. Koloshein ◽  
Sergei G. Izmailov ◽  
Egor Е. Lukoianychev ◽  
Andrey Е. Leontev ◽  
Alexey А. Bodrov ◽  
...  
Keyword(s):  
Wound Healing ◽  
Defense Mechanisms ◽  
Specific Resistance ◽  
Effective Means ◽  
The Body ◽  
Natural Resistance ◽  
Surgical Patients ◽  
Wound Complications ◽  
Nonspecific Resistance ◽  
The Impact

Importance. Surgical procedures have a lot of stress, causing significant violation of the non specific defense mechanisms of the body and the immune system, which requires the use of different pharmacological agents in order to increase natural resistance to infection.Purpose. An analytical review of experimental and clinical research in the direction of studying the effectiveness of the impact of enteral xymedon on reparative process of surgical wounds and tissue non-specific resistance of the organism to infection for the prevention of inflammatory wound complications.Materials and methods. The analysis of experimental and clinical data, dealing with the question of studying the effect of enteral and topical xymedon to repair tissue in the area of the surgical wound and the non-specific resistance to infection.The criteria for the effectiveness of the drug were clinical and laboratory parameters, and statistic methods.Results and their discussion. The analysis of experimental and clinical studies have shown that enteral and topical use xymedon in surgical patients stimulates wound healing surgical site and increases nonspecific resistance to infection by increasing the functional activity of neutrophils, their backup potentials normalize the concentration of a bioactive and immunoreactive fibronectin in blood plasma, improve antimicrobial activity of wound.Conclusions. Xymedon is recommended as an effective means of pharmacological support of wound healing in surgical patients to prevent postoperative wound complications.

scholarly journals Natural Infection of Dairy Cows with Bovine Leukemia Virus Affects Immunoglobulin Levels in Saliva and Serum but Not Milk

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 907
Author(s):  
Monika Dziuba ◽  
Vickie J. Ruggiero ◽  
Catherine Wilson ◽  
Paul C. Bartlett ◽  
Paul M. Coussens
Keyword(s):  
Pilot Study ◽  
Dairy Cows ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Natural Infection ◽  
Total Serum ◽  
Serum Samples ◽  
Retroviral Infection ◽  
Serum Iga ◽  
The Impact

Bovine leukemia virus (BLV) is a retroviral infection that disrupts the immune function of infected animals. It is widespread among U.S. dairy cattle. In this pilot study, the average total IgA and IgM concentrations in milk, saliva, and serum samples from BLV ELISA-positive (ELISA+) dairy cows were compared against samples from BLV ELISA-negative (ELISA−) cows using the Kruskal–Wallis test (with ties). The results from ELISA+ cows were also stratified by lymphocyte count (LC) and proviral load (PVL). In milk and saliva from ELISA+ cows, the average total IgA and IgM concentrations were decreased compared to ELISA− cows, although this was only statistically significant for saliva IgM in cows with low PVL (p = 0.0424). Numerically, the average total IgA concentrations were 33.6% lower in milk and 23.7% lower in saliva, and the average total IgM concentrations were 42.4% lower in milk and 15.5% lower in saliva. No significant differences were observed in the total serum IgA concentrations, regardless of PVL and LC. The total serum IgM from ELISA+ cows was significantly decreased (p = 0.0223), with the largest decreases occurring in the highest PVL and LC subgroups. This pilot study is a first step in investigating the impact of BLV on mucosal immunity and will require further exploration in each of the various stages of disease progression.

scholarly journals EFFECTIVENESS OF MICROELEMENTS IN THE FORMATION OF MECHANISMS OF PROTECTION OF THE BODY UNDER THE INFLUENCE OF ENVIRONMENTAL STRESS FACTORS

2021 ◽  
Vol 22 (1) ◽  
pp. 61-67
Author(s):  
V. O. Velichko
Keyword(s):  
Trace Elements ◽  
Immune Status ◽  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Stress Factors ◽  
Enzymatic System ◽  
Antioxidant Protection ◽  
The Impact ◽  
Active Substances

Analysis of literature data and the results of our own research show that even with intensive rearing of animals with the use of balanced feeding and keeping them in accordance with the technology - it is almost impossible to avoid stress. And especially, excessive man-made load on agroecosystems also has a negative impact on animal life. Factors that cause a decrease in immune status and the emergence of immune pathology in animals include: industrial technology of animal husbandry, chemicalization in crop and livestock production, man-made pressure, dietary imbalance in nutrients and biologically active substances. This increases the impact on the body of heavy metals, which displace nutrients from body tissues, in particular trace elements and replace them in metabolic processes, which is a potential prerequisite for the development of oxidative stress. The mechanism of development of stress reaction of an organism is closely connected with activity of POL (lipid peroxidation) and depression of antioxidant potential. Under these conditions, the ability of the organism to mobilize protective and adaptive capabilities in response to the action of negative factors becomes especially important. Keeping productive animals in adverse conditions, unbalanced feeding are components of immobilization stress, which reduces their productivity, affects reproductive function, metabolic and functional disorders, reducing nonspecific and specific resistance of the organism (Fedoruk & Kravtsiv, 2003; Velychko, 2008; Velychko, 2011). Under conditions of man-caused load on the environment, respectively, and animals – it is promising to develop effective methods for regulating the activity of the enzymatic system of antioxidant protection with the help of biologically active substances, in particular trace elements. The results of research confirm that this has a positive effect on the formation of productive and adaptive properties of animals. The system of antioxidant protection is a necessary part of non-specific reactions of the organism, a component of the processes of its adaptation to environmental conditions, a component of normal life, a factor in maintaining homeostasis. Widespread immunodeficiency and elucidation of the main links of their pathogenesis have exacerbated the problem of regulating disorders of the immune system. Therefore, knowledge of the patterns of formation of the immune status of animals, especially in the early postnatal period, is important in the development of methods for the correction of immunodeficiency, antioxidant protection.

scholarly journals In Vitro 1H NMR Metabolic Profiles of Liver, Brain, and Serum in Rats After Chronic Consumption of Alcohol

2021 ◽  
Author(s):  
Mariya S. Pravdivtseva ◽  
Oleg B. Shevelev ◽  
Vadim V. Yanshole ◽  
Mikhail P. Moshkin ◽  
Igor V. Koptyug ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Ethanol Solution ◽  
The Body ◽  
Control Group ◽  
Water Control ◽  
Serum Samples ◽  
Wide Range ◽  
The Impact ◽  
The Brain

AbstractThe impact of alcohol on the body can be investigated with NMR spectroscopy in vitro, which can detect a wide range of metabolites but preparing samples includes tissue biopsy. Blood sampling is less invasive, but blood metabolic content might not reflect the changes occurring in other tissues. Thus, this study aimed to investigate the liver, brain, and serum metabolism and evaluate the link between tissues and serum metabolic content. Two experimental groups with ten outbred rats each were provided intragastrically with water (control group) and 50% ethanol solution (alcohol group) for 28 days. 1H NMR spectroscopy in vitro was performed on the brain cortex, liver, and serum samples. Student’s t test with Holm–Bonferroni correction was used to investigate significant differences between groups. Partial least-squares discriminant analysis (PLS-DA) and two-way ANOVA were performed to compare liver and serum, brain and serum. In all, 38, 37, and 21 metabolites were identified in the liver, brain, and serum samples, respectively. Significant differences for three metabolites were found in the liver (alanine, proline, and glutathione, p < 0.002) and four in serum (lactate, betaine, acetate, and formic acid, p < 0.002) were detected between the control and alcohol groups. The contents of glucose, betaine, and isoleucine were correlated (r > 0.65) between serum and liver samples. PLS-DA determined separation between all tissues (p < 0.001) and between control and alcohol groups only for liver and serum (p < 0.001). Alcohol had a more substantial effect on liver and serum metabolism than on the brain.

scholarly journals EFFECT OF PROBIOTIC "LACTOBACTERIN-TK2" ON IMMUNOBIOLOGICAL STATUS OF NEWBORN CALVES IN THE THERAPEUTIC AND PROPHYLACTIC OF GASTROINTESTINAL DISEASES

2020 ◽  
Vol 1 (383) ◽  
pp. 26-32
Author(s):  
T. K. Boranbayeva ◽  
Zh. K.Tulemissova ◽  
S. Ozkaya ◽  
D. M. Khussainov
Keyword(s):  
Complete Recovery ◽  
Gastrointestinal Diseases ◽  
The Body ◽  
Modern World ◽  
Nonspecific Resistance ◽  
Alkaline Phospha ◽  
Newborn Calves ◽  
The Moment ◽  
The Impact ◽  
Adaptive Reactions

In recent decades, the loss of newborn calves occurs mainly from non-infectious diseases. It is established that in the process of growth and development, especially after birth, the animal has to experience the action of anthropogenic environmental factors that cause various kinds of adaptive reactions of the body. Currently, probiotic drugs are increasingly evaluated not only for their antimicrobial action but also for the characteristics of the impact on the body and its microbiota in the development of the need to strengthen the immune response, in the treatment and treatment of calf diarrhoea. It should be noted that in the modern world, a special place is occupied by studies of lactic acid bacteria associated with the development of biological products with probiotic function. The article presents the results of studying the therapeutic and prophylactic effect of the probiotic drug "Lactobacterin-TK2" in gastrointestinal diseases of newborn calves and its effect on the immune and biological status. According to the results of the study, it was found that daily feeding of the probiotic drug "Lactobacterin-TK2" to newborn calves from the moment of birth 2 times a day for 10 days increases the nonspecific resistance of the body and the safety of calves. Haematological and biochemical analysis of experimental groups of calves showed a faster normalisation of the level of alkaline phospha-tase, glucose, urea, magnesium, iron, potassium, calcium, and phosphorus, which also indicates a complete recovery.

scholarly journals Experimental evaluation of the impact of low doses of the herbicide 2,4-D in drinking water on some indices of lipid and immune status

2019 ◽  
Vol 95 (5) ◽  
pp. 450-454
Author(s):  
Larisa A. Chesnokova ◽  
I. V. Mikhaylova ◽  
I. P. Voronkova ◽  
D. S. Karmanova
Keyword(s):  
Potable Water ◽  
Ldl Cholesterol ◽  
Immune Status ◽  
The Body ◽  
Low Doses ◽  
Different Types ◽  
Calorie Diet ◽  
The Impact ◽  
High Calorie Diet ◽  
High Calorie

There were studied features of the manifestation of incoming with potable water nontoxic doses of the herbicide 2,4-D on the body mass index, some indices of lipid metabolism and immune system in different types of diets in the experiment in rats. There was shown a significant gain in body weight of animals, and MDA and leptin level in the serum under the action of a herbicide in conjunction with a high-calorie diet. In all experimental groups there was noted the increased level of total cholesterol, tendency to the increase of LDL cholesterol. Under the action of the herbicide there was noted an increase of IL-6, TNF-a and the numbers of leukocytes and, on the contrary, the reduction of the number of thymocytes and kariocytes in thymus and spleen.

scholarly journals Change of indexes of breathing and circulation of blood at loading of step - rising power for young swimmers

2019 ◽  
pp. 10-13
Author(s):  
N. Balamutova ◽  
E. Bloshenko ◽  
N. Boreyko
Keyword(s):  
Motor Activity ◽  
Functional State ◽  
Information Technologies ◽  
Rapid Development ◽  
Effective Means ◽  
Physical Activities ◽  
The Body ◽  
External Respiration ◽  
Rising Power ◽  
The Impact

The positive is well – proven numerous researches influence swimming on the health of occupying. Therefore question about affecting of employments swimming organism of young swimmers it is necessary to examine taking into account individual of anatomical and physiological features. The aim of the real research was a study of influence of physical activities of step - rising power on breathing and circulation of blood of youths 15-16, regularly visitant a section on swimming. A result of research of indexes of breathing and circulation of blood at the set loading allows describing functional possibilities of youths - swimmers. It was well - proven that the system of preparation of young swimmers was built taking into account age anatomical and physiological features of their organism, and physical activities were adequate to their physiological development. Modern living conditions are characterized by rapid development of automation of production and information technologies, which deprives a person of motor activity. Swimming is one of the most effective means of motor activity that positively influences the functional state of the cardiovascular and respiratory system. Numerous studies have already shown the positive effect of swimming on the health of the practitioners. It should not be forgotten that despite the significant differences in the functional state of the apparatus of external respiration and blood circulation in young swimmers and their peers - not athletes, the organism in both of them has not yet reached maturity and their age development and formation occurs in accordance with a single general biological regularities. Therefore, the question of the impact of swimming lessons on the body of young swimmers should be considered not only in terms of the impact on it of the specific features of the aquatic environment and the requirements of modern training, but also taking into account anatomical and physiological features.

scholarly journals Increase of Nonspecific Immunity and Productivity of Piglets when using the Probiotic Lactobacillus reuteri

2021 ◽  
pp. 121-127
Author(s):  
Ovcharova Anastasiya ◽  
Ostrenko Konstantin ◽  
Kutin Ivan
Keyword(s):  
High Efficiency ◽  
Immune Status ◽  
Lactobacillus Reuteri ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Negative Consequences ◽  
Nonspecific Resistance ◽  
Weaning Piglets ◽  
Experimental Group

The stress of weaning piglets negatively affects all factors of the body. First of all, this affects the violation of nutrition and a decrease in the activity of the immune system. The use of probiotics allows to reduce the negative consequences caused by weaning from a sow. The aim of the study was to study the effect of probiotics based on Lactobacillus reuteri on the indicators of nonspecific resistance and productivity of piglets. The obtained data showed a high efficiency of the use of probiotics. In the experimental group, the phagocytic activity of neutrophils increased by 4.4%, the phagocytic index was 6.6% higher, and the bactericidal activity increased by 29% compared to the control group. In piglets treated with Lactobacillus reuteri, body weight increased by 9.3%, and the average daily gains in the control group increased by 15.8%. The use of probiotics in the period of stress after weaning allows you to activate the immune status.

scholarly journals IMMUNE AND METABOLIC DISTURBANCES IN EXPERIMENTAL ACUTE TOXIC HEPATITIS: CORRECTION BY XENOGENIC AND ALLOGENIC HEPATOCYTES

2016 ◽  
Vol 18 (2) ◽  
pp. 91-98 ◽  
Author(s):  
A. I. Konoplya ◽  
E. S. Litvinova ◽  
N. A. Bystrova ◽  
M. S. Razumova ◽  
T. V. Chuyeva
Keyword(s):  
Carbon Tetrachloride ◽  
Metabolic Activity ◽  
Toxic Hepatitis ◽  
Liver Toxicity ◽  
Effective Means ◽  
Free Radical Oxidation ◽  
Metabolic Disturbances ◽  
Acute Toxic Hepatitis ◽  
The Impact ◽  
Acute Toxic

For correspondence: Konoplya Alexander Ivanovich. Address: 3,K. Marx St.,Kursk, 305041,Russian Federation. Tel.: job. (4712) 58-81-76; mob. (910) 317-87-88. E-mail: [email protected]. To study the corrective effects of allogeneic and xenogeneic hepatocytes on metabolic disturbances in acute liver toxicity.Material and methods. Investigations were carried out on 75 adult male Wistar rats weighing 120–160 g, 15 rats and 25 mice on the 5–6th days after birth. Acute toxic hepatitis (ATH) was modeled by intramuscular injection of carbon tetrachloride at a dose of 3 ml / kg as a 50% solution in olive oil, five times at 24-hour intervals. Isolating xenogeneic (mouse) and allogeneic hepatocytes was performed by method of Berry M.N., Friend D.S. The cell suspension was prepared daily and administered at a concentration of 2 × 106 /kg in recipients with ATH intraperitoneally, five times at 24-hour intervals, simultaneously with the first injection of hepatotropic poison.Results. Intoxication by carbon tetrachloride causes development of the biochemical syndromes of liver damage, activation of the functional metabolic activity of peripheral blood neutrophils and free-radical oxidation, breaks intraerythrocytic metabolism. The introduction of allogeneic hepatocytes in recipients with toxic hepatopathy is more efficiently compared with xenogeneic hepatocytes, it corrects local and systemic metabolic disturbances arising due to the impact of hepatotropic poison. Conclusion. Transplantation of xenogenic hepatocytes, and, to a greater extent, of allogenic hepatocytes in ATH conditions is an effective means to restore the functional metabolic activity of hepatocytes, neutrophils and erythrocytes. 

scholarly journals Study of the resistance of cows with mastitis depending on the age of lactation

2022 ◽  
Vol 354 (11-12) ◽  
pp. 27-31
Author(s):  
M. V. Dovydenkova
Keyword(s):  
Blood Serum ◽  
Immune Status ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
The Body ◽  
Nonspecific Resistance ◽  
Animal Blood ◽  
Black And White ◽  
Holstein Breed

Relevance. The breeding stock and young cattle in the conditions of industrial technology of animal husbandry experience an increased antigenic load, which leads to disturbances in the immune status and the development of inflammatory processes manifested by endometritis and mastitis, diarrheal and respiratory syndromes. It was relevant to conduct a comprehensive assessment of the immunological reactivity of the animal’s body according to the state of resistance in cattle with clinical and subclinical mastitis, depending on the age of lactation. As a result of complex studies, the specific and nonspecific reactivity of the body of cattle in different periods of lactation was studied.Methods. The object of the study were cows of a black-and-white Holstein breed (n = 450). Groups of animals were formed according to the age of lactation:1st lactation, 2–3rd lactation, 3–4th lactation and for health reasons: clinically healthy animals, with subclinical mastitis and clinical mastitis. Milk samples were taken from cows to count the number of somatic cells, and blood to determine the quantitative content of IgG immunoglobulin and indicators of nonspecific resistance.Results. It was found that with mastitis in cows of different lactation ages, almost identical changes in the immune status were observed. Quantitative determination of IgG levels in animal blood serum is the basis for assessing the immunity of cattle. The immune status in clinically healthy animals was characterized by a higher level of the total amount of IgG immunoglobulin in the blood serum (amounted to 22.25 ± 0.70 mg/ml), whereas in cows with a subclinical form of mastitis, the level of IgG immunoglobulin decreased by 12.3%, with a clinical form of mastitis — by 17.2%. The results also showed that in cows of different lactations with subclinical and clinical mastitis, the bactericidal activity (by 10.8%) and the lysozyme activity of the blood increased significantly (by 8.22%). In cows with clinical mastitis — by 8.6% and 9.7% respectively, compared with the indicators in groups of healthy animals. Thus, mastitis, occurring in acute and subacute form, leads to a decrease in the resistance of the cows’ organism.

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