scholarly journals Implementation of Meniran Extract (Phyllanthus Niruri Linn) on the Performance of Broiler Chickens Infected by Mycoplasma gallisepticum Caused Chronic Respiratory Disease

2017 ◽  
Vol 3 (6) ◽  
pp. 296
Author(s):  
Sri Hidanah ◽  
Emy Koestanti Sabdoningrum ◽  
Retno Sri Wahjuni ◽  
Arimbi Arimbi
Keyword(s):  
Respiratory Disease ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Mycoplasma Gallisepticum ◽  
Chronic Respiratory Disease ◽  
Economic Losses ◽  
Feed Conversion ◽  
Inhibit Protein Synthesis ◽  
Phyllanthus Niruri ◽  
The World

Chronic respiratory disease (CRD) of chicken is a disease that has great economic losses in poultry industry in the world. The losses are mainly due to the decrease of body weight gain, feed efficiencies, hatchabilities and increase conversion of the feed, of embryo mortality. The main causative agent of Chronic Respiratory Disease (CRD) is Mycoplasma gallisepticum. Mycoplasma gallisepticum attacks the respiratory tract, especially in young broiler chickens with age ranged 3-5 weeks. CRD treatment usually uses macrolide antibiotics, because it has proven effective to inhibit protein synthesis. However, it is not recommended to continuously given because the chicken can be resistant to the medicineand leave a harmful reside to consumers. The development of herbal medicine utilization currently is mostly implemented for the treatment of diseases that infected livestock. Meniran plants (Phyllanthus niruri Linn) is one of the plants that can be used as prevention and alternative treatment caused by Chronic Respiratory Disease (CRD). Meniran (Phyllanthus niruri Linn) has the content of bioactive compounds that have antibacterial activity, including terpenoids,alkaloids, flavonoids, saponins, and tannins. The purpose of this study is to test and evaluate the effectiveness of Meniran extract (Phyllanthus Niruri Linn) on the performance of broiler chickens infected by Chronic Respiratory Disease (CRD), seen from the feed conversion.Keywords: Meniran, Mycoplasma galisepticum, Chronic Respiratory Disease (CRD), performance of Broiler Chickens, Feed Conversion

scholarly journals An In Vitro Antibacterial Activity Test of Meniran Herbs’(Phyllanthus Niruri L.) Ethanol ExtractAgainst Mycoplasma gallisepticum causes Chronic Respiratory Disease (CRD) in Broiler Chickens

2017 ◽  
Vol 3 (6) ◽  
pp. 48 ◽  
Author(s):  
Emy Koestanti Sabdoningrum ◽  
Sri Hidanah ◽  
Retno Sri Wahjuni ◽  
Sri Chusniati ◽  
Arimbi Arimbi
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Respiratory Disease ◽  
Broiler Chickens ◽  
Inhibitory Concentration ◽  
Mycoplasma Gallisepticum ◽  
Chronic Respiratory Disease ◽  
Economic Losses ◽  
Dilution Method ◽  
Phyllanthus Niruri

Chronic Respiratory Disease (CRD) is a chicken respiratory disease that attacks both broilers and layers. Chronic Respiratory Disease (CRD) has important economic significance in the intensification of chicken farms because this disease can cause huge economic losses. Meniran plant (Phyllanthus niruri Linn) is one of the plants that can be used as prevention and alternative treatment as a substitute of antibiotic caused by Mycoplasma galisepticum causes Chronic Respiratory Disease (CRD) in broiler chickens. The chemicals contained in meniran include tannins, saponins, alkaloids as antibacterials. The purpose of this study is to determine the activity of meniran herbs’ (Phyllanthus Niruri Linn) as antibacterial to eradicate Mycoplasma galisepticum. The method of this study is dilution method which included Minimum Inhibitory Concentration [MIC) and Minimum Bactericidal Concentration (MBC). Minimum Inhibitory Concentration [MIC) was taken by making the concentration of meniran extract as much 65%, 62,5%; 60%; 55%; 50%; 45%; 40%. It was then added Mycoplasma gallisepticum bacteria. The result of this study is Meniran’s activation test on Mycoplasma galisepticum obtained a dose of 62,5% could eradicate Mycoplasma galisepticum causes Chronic Respiratory Disease (CRD) in broiler chickens. Meniran herbs’ (Phyllanthus niruri linn) is effective as antibacterial at concentrations of 30% against Mycoplasma gallisepticum causes Chronic Respiratory Disease (CRD) in broiler chickens. Keywords: Meniran herbs’ (Phyllanthus Niruri Linn), Mycoplasma Galisepticum, Chronic Respiratory Disease (CRD)

scholarly journals Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2027
Author(s):  
Doaa Ibrahim ◽  
Tamer Ahmed Ismail ◽  
Eman Khalifa ◽  
Shaimaa A. Abd El-Kader ◽  
Dalia Ibrahim Mohamed ◽  
...  
Keyword(s):  
Growth Performance ◽  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Economic Benefits ◽  
Production Costs ◽  
Broiler Chicks ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

scholarly journals Effects of Lactobacillus reuteri and Streptomyces coelicolor on Growth Performance of Broiler Chickens

2021 ◽  
Vol 9 (6) ◽  
pp. 1341
Author(s):  
Sarayu Bhogoju ◽  
Collins N. Khwatenge ◽  
Thyneice Taylor-Bowden ◽  
Gabriel Akerele ◽  
Boniface M. Kimathi ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Lactobacillus Reuteri ◽  
Body Weight Gain ◽  
Streptomyces Coelicolor ◽  
Control Diet ◽  
Probiotic Bacteria ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Wheat Middlings ◽  
Dietary Treatments

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of Lactobacillus reuteri (L. reuteri) and Actinobacteria class of bacteria in their gastrointestinal tract. In this study Lactobacillus reuteri and Streptomyces coelicolor (S. coelicolor) were selected as probiotic bacteria, encapsulated, and added into broiler feed at a concentration of 100 mg/kg of feed. In an 8-week study, 240 one day-old chicks were randomly assigned to four dietary treatments. Three dietary treatments contained two probiotic bacteria in three different proportions (L. reuteri and S. coelicolor individually at 100 ppm, and mixture of L. reuteri and S. coelicolor at 50 ppm each). The fourth treatment had no probiotic bacteria and it functioned as the control diet. L. reuteri and S. coelicolor were added to the feed by using wheat middlings as a carrier at a concentration of 100 ppm (100 mg/kg). Chickens fed diets containing L. reuteri and S. coelicolor mixture showed 2% improvement in body weight gain, 7% decrease in feed consumption, and 6–7% decrease in feed conversion ratios. This research suggests that L. reuteri and S. coelicolor have the potential to constitute probiotics in chickens combined or separately, depending on the desired selection of performance index.

scholarly journals The Effect of Dietary Halloysite Supplementation on the Performance of Broiler Chickens and Broiler House Environmental Parameters

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2040
Author(s):  
Małgorzata Nadziakiewicz ◽  
Marcin Wojciech Lis ◽  
Piotr Micek
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Environmental Parameters ◽  
Feed Consumption ◽  
Feed Additive ◽  
Feed Conversion ◽  
Daily Body Weight ◽  
Daily Water ◽  
Broiler House

The aim of the study was to determine the effect of supplementing broiler chickens’ diets with halloysite on daily body weight gain (BWG), feed conversion ratio (FCR), daily water consumption (DWC), and some broiler house hygiene parameters. The trial was conducted on 18,000 broiler chickens divided into two groups throughout the 42-day (D) rearing period. The birds were fed complete diets without (group C) or with halloysite addition (1%, group E) from D8 of rearing. No difference in the mortality rate was observed between groups C and E. Birds from group E had a tendency (0.05 < p < 0.10) towards a higher body weight at D32 and D42, a higher BWG, and a lower FCR compared to group C during the entire rearing period. Average DWC differed only in the finisher period, with a tendency towards lower overall DWC in group E. The concentration of ammonia in the air from D21 to D35 was increased more than 5-fold in group C but only 1.5-fold in group E. In conclusion, the use of halloysite as a feed additive in the diet of broiler chickens resulted in a reduction in feed consumption per unit of BWG and higher utilisation of crude protein, which led to improved environmental conditions.

scholarly journals Growth Performance, Serum Biochemical Indices, Duodenal Histomorphology, and Cecal Microbiota of Broiler Chickens Fed on Diets Supplemented with Cinnamon Bark Powder at Prestarter and Starter Phases

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Serum Globulin ◽  
Maximum Improvement

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.

scholarly journals Evaluasi Performa dan Income Over Feed & Chick Cost (IOFCC) Tiga Strain Ayam Broiler yang Beredar di Aceh

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Zulfan Zulfan ◽  
Zulfikar Zulfikar
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Economic Value ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Randomized Design ◽  
Different Strains ◽  
Better Than

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi performa tiga strain ayam broiler yang umumnya dipelihara di Aceh. Materi yang digunakan adalah 150 ekor anak ayam broiler (DOC) strain Cobb 500, CP 707, dan MB 202 masing-masing berjumlah 50 ekor. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Setiap ulangan merupakan unit percobaan yang masing-masing terdiri dari 10 ekor anak ayam. Perlakuan adalah tiga strain ayam broiler: Cobb 500 (P1), CP 707 (P2), dan MB 202 (P3). Parameter yang diamati meliputi pertambahan berat badan, konsumsi ransum, konversi ransum (FCR), mortalitas, dan Income over Feed and Chick Cost (IOFCC). Hasil penelitian memperlihatkan performa broiler dipengaruhi oleh jenis strain. Strain MB 202 dan CP 707 memiliki bobot badan akhir (6 minggu) dan konsumsi ransum nyata (P0,05) lebih tinggi daripada Cobb 500. Angka konversi ransum tidak memperlihatkan perbedaan yang nyata antara ketiga strain pada semua umur panen. Nilai IOFCC tertinggi dipengaruhi oleh jenis strain dan lama pemeliharaan. Dari hasil penelitian dapat disimpulkan bahwa performa broiler dipengaruhi oleh kemampuan adaptasi strain terhadap iklim setempat (pesisir Aceh) pada periode berbeda-beda yang dapat memengaruhi tingkat keuntungannya. Strain Cobb 500 memiliki kemampuan adaptasi lebih baik selama periode awal, sedangkan CP 707 dan MB 202 selama periode akhir. (Evaluation of performances and income over feed chick cost (iofcc) of three broiler chicken strains commonly marketed in Aceh) ABSTRACT. The study aimed to evaluate the performances and Income over Feed and Chick Cost (IOFCC) of three broiler chicken strains commonly marketed in Aceh. As many as 150 chicks of three different broiler strains (50 birds each) were used in this study. The study was performed into Completely Randomized Design (CRD) consists of 3 treatments and 5 replications creating 15 experimental units containing 10 birds each. The treatments were 3 different strains of broiler chickens i.e. Cobb 500, CP 707, and MB 202. The birds were reared up to 6 weeks to expose their performances recorded as final body weight, body weight gain, feed intake, and feed conversion, as well as mortality. Economic value was evaluated by calculating IOFCC. Results of study showed that performances of broilers were significantly (P0,05) influenced by the strains. Strains MB 202 and CP 707 represented more superior than Cobb 500 for 5−6 weeks of raising periods. However, the later was not inferior during initial phase close to 4 weeks. There were no significant differences in FCR among all strains for different ages. The IOFCC related to the strains and their marketing ages. The highest IOFCC was obtained by Cobb 500, CP 707, and MB 202 as they were sold at the ages of 4, 5, and 6 weeks, respectively. In conclusion, the achievements of the performances in various broiler strains were affected by the site climate (Aceh) in different stages of their ages. Cobb 500 had proper adaptation during starter, while CP 707 and MB 202 appeared seem to be better than Cobb 500 during finisher period.

Performance and slaughter characteristics of broiler chickens fed different sources of maggot meal

2020 ◽  
Vol 44 (2) ◽  
pp. 262-270
Author(s):  
O. O. Egbewande ◽  
A. A. Jimoh ◽  
B. Y. Amore ◽  
B. Y. Amore
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Fish Meal ◽  
Body Weight Gain ◽  
Proximate Analysis ◽  
Feed Conversion ◽  
Performance Indices ◽  
Randomized Design ◽  
Different Sources

In this study, the effect of three different sources of maggot meals was conducted to evaluate the performance and slaughter characteristics of broiler chickens. Five diets were formulated to include Diet 1 (control) which contained 3.5 % fish meal, while Diets 2, 3 and 4 contained 3.5% maggot meal from broiler droppings (BMM),maggot meal from layers droppings (LMM) and maggot meal from rabbit faeces (RMM) respectively. Eighty (80) dayold unsexed Marshall Broilers were randomly allotted to four dietary treatments in a completely randomized design (CRD). Thedietary treatments had 20 birds each with two (2) replicates. Data were generated on proximate analysis and performance indices including feed intake and weight gain, slaughter characteristics and mortality rate. They were subjected to analysis of variance (ANOVA) and means significantly different were separated using Duncan's Multiple Range Test (DMRT) at 5% probability level. Results from proximate analysis showed that maggots from layer droppings had the highest crude protein and ether extract. Results on performance indices showed that birds fed on diet 3 (layer maggot meal) had significantly (P<0.05) best performance than others as regards feed intake. Layer maggot meal (LMM) in this study enhanced bestproductive performance compared to those from broiler and rabbit maggot.Birds fed layer maggot meal had the best (1.85) feed conversion ratio. They also had the best body weight gain (2501g) and feed intake (4627g). In conclusion, layer maggot meal is recommended as analternative animal protein source to expensive fish meal.

scholarly journals Combination of Bacillus licheniformis and Salinomycin: Effect on the Growth Performance and GIT Microbial Populations of Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 889 ◽  
Author(s):  
Jacek Trela ◽  
Bartosz Kierończyk ◽  
Veerle Hautekiet ◽  
Damian Józefiak
Keyword(s):  
Growth Performance ◽  
Bacillus Licheniformis ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Alpha Diversity ◽  
Feed Conversion ◽  
Beneficial Effects ◽  
Ph Values ◽  
Combined Use ◽  
Dietary Treatments

The aim of the study was to investigate the effect of Bacillus licheniformis and salinomycin supplementation in broiler diets as individual factors or in combination on the growth performance, GIT morphometry, and microbiota populations. Four hundred one-day-old Ross 308 chicks were randomly distributed to four dietary treatments (10 replicates, 10 birds each). The following treatments were applied: NC—no additives; NC + SAL—salinomycin addition (60 mg/kg diet), NC + PRO—B. licheniformis DSM 28710 preparation (1.6 × 109 CFU/kg; 500 mg/kg diet), and NC + SAL + PRO—combination of salinomycin and B. licheniformis. Probiotic administration resulted in improvement (p < 0.05) of the performance parameters, including body weight gain (1–10 d, and 11–22 d) and feed conversion ratio (11–22 d, 1–36 d). An interaction (p < 0.05) between experimental factors was observed in terms of lower pH values in the crop (tendency, p = 0.053) and ceca. Both factors lowered the alpha diversity and Enterobacteriaceae and promoted Bacillaceae communities in the jejunum (p < 0.05). Interactions were also observed in terms of reducing Clostridiaceae in the ceca. In conclusion, the combined use of B. licheniformis and salinomycin in broilers’ diets had beneficial effects.

EFFECT OF DIETARY CATION-ANION BALANCE AND CALCIUM CONTENT ON GENERAL PERFORMANCE AND INCIDENCE OF LEG ABNORMALITIES OF BROILER CHICKENS

1987 ◽  
Vol 67 (1) ◽  
pp. 165-177 ◽  
Author(s):  
H. W. HULAN ◽  
K. B. McRAE ◽  
F. G. PROUDFOOT ◽  
P. C. M. SIMONS ◽  
P. J. W. VAN SCHAGEN
Keyword(s):  
Weight Gain ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Calcium Content ◽  
Feed Utilization ◽  
Feed Conversion ◽  
Tibial Dyschondroplasia ◽  
General Performance ◽  
Feeding Diets ◽  
Main Effects

A factorial experiment with 1440 day-old Hubbard chicks was conducted to assess the interacting effects of dietary Na, K, Cl and Ca on general performance and incidence of leg abnormalities, especially tibial dyschondroplasia (TD). For Ca at 0.95%, all combinations of Na (0.17, 0.30), K (0.80, 1.10, 1.40), Cl (0.22, 0.44) were present; for Ca at 1.38% the combinations were the same except for K (0.80, 1.40). The various cation combinations produced 12 and 8 different meq kg−1 of (Na + K − Cl) for Ca at 0.95 and 1.38%, respectively. Body weight gain and feed conversion were affected by the main effects K, Ca and Na and also by their interaction. Both weight gain and efficiency of feed utilization were influenced by cation-anion balance as shown by significant Ca × K, Na × K, Ca × Na (feed conversion only) and K × Cl interactions. Increasing dietary K or Na increased litter moisture, whereas Cl moderated the effect of Ca. The incidence of TD was reduced by increasing dietary Ca, Na or K but their effects are interdependent on each other and on the level of dietary Cl as evidenced by significant Ca × Na, Na × K and Na × K × Cl interactions. Feeding diets with (Na + K − Cl) between 155 and 300 meq kg−1 had little effect on growth and feed conversion. Dietary Ca level changed both the pattern and response of birds for growth and feed utilization, suggesting that the term meq kg−1 (Na + K − Cl) is an inadequate measure of dietary cation-anion balance. Key words: Dietary cation-anion, calcium, leg abnormalities, broilers

scholarly journals Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

2019 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Cristiano Bortoluzzi ◽  
Bruno Serpa Vieira ◽  
Juliano Cesar de Paula Dorigam ◽  
Anita Menconi ◽  
Adebayo Sokale ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Clostridium Perfringens ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Dietary Supplementation ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Negative Effects ◽  
Infected Control

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

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