scholarly journals Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferous Tubules in Male Mice (Mus musculus)

2017 ◽  
Vol 3 (6) ◽  
pp. 677
Author(s):  
Dona Astari Nurkarimah ◽  
Eka Pramyrtha Hestianah ◽  
Retno Sri Wahjuni ◽  
Mas’ud Hariadi ◽  
Suryo Kuncorojakti ◽  
...  
Keyword(s):  
Oral Administration ◽  
Mus Musculus ◽  
Seminiferous Tubule ◽  
Ethanolic Extract ◽  
Lower Number ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Adult Mice

The present research aimed to determine the effect of propolis in spermatogenic cells number and seminiferous tubules diameter. Group P0 served as control group, P1 (1,6 mg/0,5ml/day), P2 (3,2 mg/0,5 ml/day), P3 (6,4 mg/0,5 ml/day), and P4 (12,8 mg/0,5 ml/day) were given propolis ethanolic extract treatment orally and killed after 14 days. Spermatogenic cells number (spermatogonial cells, primary spermatocytes, spermatids) and seminiferous tubules diameter were observed. The result showed a lower number of spermatogonial in P1 and P2 groups, primary spermatocytes reduction in P2 group, spermatids increased in P1, P3, and P4 group. Seminiferous tubules diameter decreased in P2 and P3 group. P2 group (3,2 mg/0,5 ml/day) showed the lowest result in all parameters (p<0.05). However, oral administration of propolis at these dose for 14 days may decrease spermatogonial cells and primary spermatocytes and increase spermatids number in adult mice.  Keywords: Propolis; seminiferous tubule; spermatogenic cells; testis histology

scholarly journals THE EFFECT OF RED DRAGON FRUIT SKIN EXTRACT (Hylocereus polyrhizus) ON THE NUMBER OF LEYDIG CELLS, DIAMETER OF SEMINIFEROUS TUBULES, AND TESTICULAR WEIGHT OF MALE MICE (Mus musculus) EXPOSURED WITH HOT TEMPERATURES

2021 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Kukuh Prastyaningtyas ◽  
Rochmah Kurnijasanti ◽  
Rahmi Sugihartuti ◽  
Suherni Susilowati ◽  
Tri Wahyu Suprayogi ◽  
...  
Keyword(s):  
Mus Musculus ◽  
Leydig Cells ◽  
Heat Exposure ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Skin Extract ◽  
Male Mice ◽  
Fruit Peel ◽  
Testicular Weight ◽  
Hylocereus Polyrhizus

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received a placebo. Meanwhile, the treatment groups mice were exposed to heat for 45 minutes daily for 36 days and oral administration of placebo, RDFPE of 250, 500, and 1000mg/kg BW for T0, T1, T2, and T3, respectively. The result showed that heat exposure on mice (T0 group) caused a lower of all of the parameters (p <0.05) than normal mice (control group, C). RDFPE administration at a dose of 250 mg/kg BW (T1 group) and 500 mg/kg BW (T2 group) resulted in a higher value of those parameters (p <0.05) compared to the T0 group. All those parameters of the T2 group (dose of 500 mg/kg BW) were not significantly different (p >0.05) than the control group (normal mice). However, the higher dose of RDFPE (1000 mg/kg BW, T3 group) resulted in the lower values of those parameters (p <0.05) than those of the T2 group. It could be concluded that 500mg/kg BW dose of RDFPE could return Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat.

scholarly journals PENGARUH PEMBERIAN JAMUR Fusarium graminearum TERHADAP HISTOPATOLOGI TUBULUS SEMINIFERUS MENCIT (Mus musculus)

2020 ◽  
Vol 8 (1) ◽  
pp. 54
Author(s):  
Fierda Kabayo ◽  
Abdul Samik ◽  
Ismudiono Ismudiono ◽  
Tjuk Imam Restiadi ◽  
Soeharsono Soeharsono ◽  
...  
Keyword(s):  
Body Weight ◽  
Veterinary Medicine ◽  
Sodium Chloride ◽  
Fusarium Graminearum ◽  
Mus Musculus ◽  
Histopathological Examination ◽  
Science And Technology ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice

The aim of this research was to show the influence of seminiferous tubules histopathology of mice (Mus musculus) caused Fusarium graminearumexposure. This research was done in April-May 2017 in Microbilogy Laboratory Faculty of Science and Technology, Animal Laboratory, and Microbilogy Laboratory Faculty of Veterinary Medicine UniversitasAirlangga. This research used 20 male mice (Mus musculus) aged 6 weeks with 18-30 gram body weight. The mice devided into four groups: P0 given 0,25 ml Sodium chloride without Fusarium graminearum exposure by oral; P1 given 0,25 ml Fusarium graminearum exposure with dilution 102 by oral; P2 given 0,25 ml Fusarium graminearum exposure with dilution 103 by oral; and P3 given 0,25 ml Fusarium graminearum exposure with dilution 104 by oral. This treatment carried out for 21 days. Each milliliter dilution containing 228x106 spore for P1, 228x107 spore for P2, and 228x108 spore for P3. Then do the surgery and harvesting the testes then performed histopathological examination by scoring of seminiferous tubules. For data analyzing used non parametric difference Kruskall-Wallis and continued with Mann-Whitney. The result of this research was showed that decreased the spermatogenic cells in the process of spermatogenesis significant.

scholarly journals Potensi ekstrak Solanum betaceum terhadap peningkatan sel spermatogenik pada mencit (Mus musculus) yang dipapar timbal asetat

2020 ◽  
Vol 9 (1) ◽  
pp. 87
Author(s):  
Nurul Fatimah Susanti ◽  
Reny I’tishom ◽  
Siti Khaerunnisa
Keyword(s):  
Treatment Group ◽  
Mus Musculus ◽  
Lead Acetate ◽  
Reproductive Tract ◽  
Total Sample ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Spermatogenic Cells ◽  
Control Group Design ◽  
Solanum Betaceum

Background : Flavanoids are antioxidants that can prevent the negative effects caused by lead. The flavonoids contained in the Solanum betaceum extract have the potential to prevent the adverse effects of lead on the reproductive tract of rats because it can prevent oxidative stress. The purpose of this study was to prove the effect of Solanum betaceum extract on spermatogenic cells of mice exposed to lead acetate. The hypothesis in this study is that there is an effect of the administration of Solanum betaceum extract to an increase in the number of spermatogenic cells of mice (Mus musculus) exposed to lead Method : this type of research was a true experimental laboratory (true experimental), the research design uses a randomized posttest only control group design approach. The total sample of 40 heads was divided into 5 groups. Group K-: control group without the provision of lead acetate and Solanum betaceum extract, group K +: group with 75 mg / KgBB of lead acetate for 32 days, group P1: group of treatment with 75 mg / KgBB of lead acetate extract + Solanum betaceum extract for 31 days + group 100 mg / gBB for 35 days, group P2: treatment group with 75 mg / kg lead acetate for 31 days + Solanum betaceum extract 200 mg / KgBB for 35 days, and group P3: treatment group with 75 mg lead acetate KgBB for 31 days + Solanum betaceum extract 400 mg / gBB for 35 days.. Results : The mean ± standard deviation of the highest cell spermatogenic was highest 2107.88±78.70.Conclusion : The administration of Solanum betaceum extract can increase the thickness of the seminiferous tubules epithelium and the diameter of the seminiferous tubules in rats (Mus musculus) which are exposed to lead acetat.

Restraint stress of male mice triggers apoptosis in spermatozoa and spermatogenic cells via activating the TNF-α system

Zygote ◽  
2020 ◽  
Vol 28 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Jie Zhang ◽  
De-Ling Kong ◽  
Bin Xiao ◽  
Hong-Jie Yuan ◽  
Qiao-Qiao Kong ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Restraint Stress ◽  
Semen Quality ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Testicular Cells ◽  
Tnf Α

SummaryStudies have indicated that psychological stress impairs human fertility and that various stressors can induce apoptosis of testicular cells. However, the mechanisms by which psychological stress on males reduces semen quality and stressors induce apoptosis in testicular cells are largely unclear. Using a psychological (restraint) stress mouse model, we tested whether male psychological stress triggers apoptosis of spermatozoa and spermatogenic cells through activating tumour necrosis factor (TNF)-α signalling. Wild-type or TNF-α−/− male mice were restrained for 48 h before examination for apoptosis and expression of TNF-α and TNF receptor 1 (TNFR1) in spermatozoa, epididymis, seminiferous tubules and spermatogenic cells. The results showed that male restraint significantly decreased fertilization rate and mitochondrial membrane potential, while increasing levels of malondialdehyde, active caspase-3, TNF-α and TNFR1 in spermatozoa. Male restraint also increased apoptosis and expression of TNF-α and TNFR1 in caudae epididymides, seminiferous tubules and spermatogenic cells. Sperm quality was also significantly impaired when spermatozoa were recovered 35 days after male restraint. The restraint-induced damage to spermatozoa, epididymis and seminiferous tubules was significantly ameliorated in TNF-α−/− mice. Furthermore, incubation with soluble TNF-α significantly reduced sperm motility and fertilizing potential. Taken together, the results demonstrated that male psychological stress induces apoptosis in spermatozoa and spermatogenic cells through activating the TNF-α system and that the stress-induced apoptosis in spermatogenic cells can be translated into impaired quality in future spermatozoa.

scholarly journals The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Salman MO ◽  
Al-Wasiti EA ◽  
Thamir KA ◽  
Al-Ani IM ◽  
Al-Salihi AR
Keyword(s):  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sham Operation ◽  
Male Mice ◽  
Histological Changes ◽  
Single Cell Gel Electrophoresis

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

scholarly journals Noise Exposure Decreases the Number of Spermatozoa in Male Mice (Mus musculus L.) Even With Grape Seed Extract (Vitis vinifera L.) Administration

2020 ◽  
Vol 5 (2) ◽  
pp. 70-75
Author(s):  
Ghestiara Siregar
Keyword(s):  
Treatment Group ◽  
Mus Musculus ◽  
Noise Exposure ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Control Group ◽  
Vitis Vinifera L ◽  
Not Given ◽  
Male Mice

  Excessive continuous noises exposure changes the male hormone system which leads to formation of oxidative stress and results in disrupt of semen quality. This condition can be reduced by the use of antioxidants. Grape seed is one of the antioxidants that contains phenol components that have Resveratrol compounds. This research aimed to observe the effect of noise exposure on the number of spermatozoa of male mice given grape seed extract. The method of the research was a post-test only control group design with research subjects of 30 male Mus musculus L. (Swiss Webster) mice divided into 5 groups: group A (treatment control) was not given noise exposure and grape seed extract, group B (negative control) was not given noise exposure and was given grape seed extract, groups C, D, E (treatment group) were given noise exposure with sequential intensities of 65 dB, 85 dB, 105 dB. The treatment was given for 33 days. The results showed that noise exposure with different intensities of 65 dB, 85 dB, 105 dB reduced the spermatozoa count of male Swiss Webster mice even with the administration of grape seed extract. One Way Anova test was used to analyze the data with p-value of 0.001. Conclusion: There were differences in spermatozoa count between the control group and the treatment group. Provision of noise exposure with a value above the threshold limit reduces the number of male Swiss Webster mice spermatozoa given with grape seed extract. Keywords: Number of Spermatozoa, Grape Seed Extract, Noise exposure  

Restraint stress of male mice induces apoptosis in spermatozoa and spermatogenic cells: role of the FasL/Fas system†

2019 ◽  
Vol 101 (1) ◽  
pp. 235-247 ◽  
Author(s):  
Bin Xiao ◽  
Xiao Li ◽  
Xiu-Yun Feng ◽  
Shuai Gong ◽  
Zhi-Bin Li ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Semen Quality ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Loss Of Function ◽  
Tnf Α ◽  
Induced Apoptosis ◽  
Necrosis Factor

AbstractThe mechanisms by which psychological stress impairs semen quality are largely unknown. By using a restraint-stressed mouse model, we studied the role of the FasL/Fas system in psychological stress-induced apoptosis of spermatozoa and spermatogenic cells. Male mice were restrained for 48 h before examination for sperm fertilizing potential and for apoptosis and FasL/Fas expression in spermatozoa, spermatogenetic cells/seminiferous tubules, and caudae epididymides. The results showed that the male restraint reduced motility, fertilization rates, and mitochondrial membrane potential while increasing apoptosis and Fas expression in spermatozoa. Restraint also facilitated apoptosis and FasL/Fas expression in spermatogenic cells/seminiferous tubules and caudae epididymides. The restraint-induced apoptosis in spermatozoa and spermatogenic cells was significantly ameliorated in gld mice that harbor a loss-of-function mutation in FasL. However, incubation with FasL did not affect sperm motility and apoptosis, while incubation with tumor necrosis factor (TNF)-α did. The epididymis of the gld mice produced significantly less TNF-α and TNF-related apoptosis-inducing ligand (TRAIL) than that of wild-type mice did after male restraint. Thus, the results confirmed that the FasL/Fas system played an important role in the psychological stress-induced apoptosis of spermatozoa and spermatogenic cells and that FasL triggered sperm apoptosis in epididymis dependently through promoting TNF-α and TRAIL secretion.

scholarly journals CIB4 is essential for the haploid phase of spermatogenesis in mice†

2020 ◽  
Vol 103 (2) ◽  
pp. 235-243
Author(s):  
Zoulan Xu ◽  
Haruhiko Miyata ◽  
Yuki Kaneda ◽  
Julio M Castaneda ◽  
Yonggang Lu ◽  
...  
Keyword(s):  
Developmental Process ◽  
Human Testis ◽  
Seminiferous Tubules ◽  
Apical Region ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Essential Function ◽  
Diploid Cells ◽  
Shed Light

Abstract Spermatogenesis is a complex developmental process that involves the proliferation of diploid cells, meiotic division, and haploid differentiation. Many genes are shown to be essential for male fertility using knockout (KO) mice; however, there still remain genes to be analyzed to elucidate their molecular mechanism and their roles in spermatogenesis. Calcium- and integrin-binding protein 1 (CIB1) is a ubiquitously expressed protein that possesses three paralogs: CIB2, CIB3, and CIB4. It is reported that Cib1 KO male mice are sterile due to impaired haploid differentiation. In this study, we discovered that Cib4 is expressed strongly in mouse and human testis and begins expression during the haploid phase of spermatogenesis in mice. To analyze the function of CIB4 in vivo, we generated Cib4 KO mice using the CRISPR/Cas9 system. Cib4 KO male mice are sterile due to impaired haploid differentiation, phenocopying Cib1 KO male mice. Spermatogenic cells isolated from seminiferous tubules demonstrate an essential function of CIB4 in the formation of the apical region of the sperm head. Further analysis of CIB4 function may shed light on the etiology of male infertility caused by spermatogenesis defects, and CIB4 could be a target for male contraceptives because of its dominant expression in the testis.

The Oleo-Gum-Resin of Commiphora myrrha Ameliorates Male Reproductive Dysfunctions in Streptozotocin-Induced Hyperglycemic Rats

2019 ◽  
Vol 25 (4) ◽  
pp. 294-302 ◽  
Author(s):  
Mohammadmehdi Hassanzadeh_Taheri ◽  
Mehran Hosseini ◽  
Davood Dorranipour ◽  
Mohammad Afshar ◽  
Hesam Moodi ◽  
...  
Keyword(s):  
Sex Hormones ◽  
Scientific Evidence ◽  
Fasting Blood Glucose ◽  
Sperm Concentration ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Histological Evaluation ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Beneficial Effects

Background: The oleo-gum-resin of Commiphora myrrha (myrrh) has a long history of therapeutic use in traditional medicine. The aim of this study was to seek for the scientific evidence to determine whether the ethanolic extract of myrrh (EEM) has any beneficial effects on Streptozotocin (STZ) induced testicular impairments, and explore the possible mechanisms underlying such actions. Methods: Forty-eight severe and complicated diabetic rats (fasting blood glucose above 350 mg/dL), randomly were divided into six equal groups (n=8). Besides, eight healthy rats allocated as a normal control group and only treated with vehicle solution. The diabetic animals orally received the extract (100, 200, 300, and 500 mg/kg), metformin (500 mg/kg) or vehicle solution for 28 days. As a final point, plasma glucose and insulin, circulatory sex hormones, sperm parameters including sperm concentration, motility and viability and also testicular malondialdehyde (MDA) levels were assessed. Furthermore, quantitative histological evaluation of seminiferous tubules area and determination of germinal cells apoptosis were performed. Results: None of the studied doses of EEM showed anti-diabetic effects. However, the extract mainly at the maximum dose (500 mg/kg) exhibited beneficial effects on reproductive impairments. The EEM treated rats mainly at 500 mg/kg had significantly higher sperm concentration, sperm motility, sperm viability, sex hormones and lower testicular MDA and germ cell apoptosis index than untreated diabetic rats. Conclusion: These results indicated that EEM may have beneficial effects against reproductive dysfunction induced by diabetes. The mechanisms behind the effects might be associated with the EEM sex hormone booster potential, antioxidant and anti-apoptotic properties.

Endocrine disrupting potential and reproductive dysfunction in male mice exposed to deltamethrin

2016 ◽  
Vol 36 (3) ◽  
pp. 218-226 ◽  
Author(s):  
A Ben Slima ◽  
Y Chtourou ◽  
M Barkallah ◽  
H Fetoui ◽  
T Boudawara ◽  
...  
Keyword(s):  
Pesticide Exposure ◽  
Semen Quality ◽  
Sperm Quality ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Cell Cytoplasm ◽  
Pregnant Females ◽  
Sperm Density ◽  
Endocrine Disrupting

Pesticide exposure may affect semen quality and male fertility in humans. The aim of the present work was to elucidate the adverse effects of deltamethrin (Delta), a synthetic pyrethroid, on exposed male mice and their offspring. Adult male Albino/Swiss mice received deltamethrin (5 mg/kg) daily for 35 days and mated with untreated females to produce offspring. Classical measurements of ejaculate and sperm quality and testicular histopathological changes were assessed. Deltamethrin treatment affects sperm quality and quantity in the ejaculated semen of mice that had also markedly impaired libido as measured by indices of mating and fertility and number of pregnant females housed with male mice exposed to this pesticide. Exposure mice to deltamethrin significantly decreased their testosterone and inhibin B levels and affected reproductive performance. Testes of exposed mice showed marked histopathological alterations as compared to the control group. The mice exposed to 5 mg/kg body weight/day of deltamethrin showed severe alterations of the seminiferous tubules, sloughing of the germ cells, the vacuolization of germ cell cytoplasm, and the disruption of spermatogenic cells compared to the control group. Altered pregnancy outcomes were directly attributed to damage of sperm of male mice exposed to deltamethrin compared to the control group. We concluded that exposure to deltamethrin affected the reproductive system of male mice explored by altered total sperm density, motility, and morphology in mice spermatozoa.

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