scholarly journals Effects of Curcumin on Iron Overload in Rats

2021 ◽  
Author(s):  
Gülüzar Özbolat ◽  
Arash Alizadeh Yegani
Keyword(s):  
Iron Overload ◽  
Blood Cells ◽  
Serum Iron ◽  
Serum Levels ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Iron Dextran ◽  
Corpuscular Hemoglobin ◽  
Group Iii ◽  
Group I

Background: Iron overload, common in patients with hematological disorders, is a key target in drug development. This study investigated the effects of curcumin on iron overload in rats. Methods: Forty male Wistar rats weighing 139.78 ± 11.95 gm (Mean ± SD) were divided into three equal groups: (i) controls; (ii) iron overload group that received six doses of iron dextran 1000 mg/kg–1 by intraperitoneal injections (i.p.); and (iii) iron overload curcumin group that received six doses of curcumin (1000 mg/kg BW by i.p.).  In addition to six doses of iron dextran 1000 mg/kg–1 by i.p., we studied the effects of curcumin on liver function enzymes (alanine aminotransferase [ALT] and aspartate aminotransferase [AST]); antioxidant enzymes (malondialdehyde [MDA], total oxidant status [TOS], total antioxidant status [TAS]); hematological parameters (hemoglobin [Hb], hematocrit [Hct], red blood cells [RBC], white blood cells [WBC], mean corpus volume [MCV], mean corpuscular hemoglobin [MCH], mean corpuscular hemoglobin concentration [MCHC]); and iron parameters (serum iron profile, transferrin, total iron-binding capacity [TIBC], ferritin, and transferrin saturation [TS%]). Results: Curcumin caused a significant decrease in the Hct and Hb concentrations in Group III (P < 0.05). It also significantly reduced the serum levels of ALT (52.45 ± 4.51 vs 89.58 ± 4.65 U/L) and AST (148.03 ± 6.47 vs 265.27 ± 13.02 U/L) at the end of the study (P < 0.05). The TIBC, transferrin levels, and TS significantly decreased when the rats were administered curcumin serum iron (P < 0.05). The TAS level significantly increased in Group III in comparison to Group I (the control group) (P < 0.05). At the end of the study, curcumin significantly reduced the serum levels of TOS (12.03 ± 2.8 vs 16.95 ± 5.05 mmol H2O2/L) while the TAS (1.98 ± 0.42 vs 1.06 ± 0.33 mmol Trolox equiv./L) was increased. Conclusion: The findings of the present study suggest the therapeutic potential of curcumin against iron overload.

scholarly journals The Use of Antifibrotic Recombinant nAG Protein in a Rat Liver Fibrosis Model

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
Maha M. Arafah ◽  
Mohammad M. Al-Qattan ◽  
Durria A. Abdulmaged-Ahmed ◽  
Ghada A. Al-Nafesah ◽  
Nessrin Y. Jadu ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Rat Liver ◽  
Serum Levels ◽  
Senior Author ◽  
Control Group ◽  
Protein Markers ◽  
Group Iii ◽  
Group I ◽  
Cord Injury ◽  
Rat Liver Fibrosis

Objectives. The “nAG” protein is the key protein mediating the regeneration of amputated limbs in salamanders. The senior author (MMA) developed the original hypothesis that since “nAG” is a “regenerative” protein, it must be also an “antifibrotic’ protein. The antifibrotic properties were later confirmed in a rabbit skin hypertrophic scar model as well as in a rat spinal cord injury model. The aim of this study is to evaluate the potential therapeutic properties of the nAG protein in a rat liver fibrosis model. Methodology. Liver fibrosis was induced using intraperitoneal injections of carbon tetrachloride (CCL4). A total of 45 rats were divided equally into 3 groups: Group I (the control group) received normal saline injections for 8 weeks, Group II received CCL4 for 8 weeks, and Group III received CCL4 and nAG for 8 weeks. At the end of the experiment, the serum levels of 6 proteins (hyaluronic acid, PDGF-AB, TIMP-1, laminin, procollagen III N-terminal peptide, and collagen IV-alpha 1 chain) were measured. Liver biopsies were also taken and the stages of live fibrosis were assessed histologically. Results. The CCL4 treatment resulted in a significant increase in the serum levels of all 6 measured proteins. The nAG treatment significantly reduced these high levels. The degree of liver fibrosis was also significantly reduced in the CCL4/nAG group compared to the CCL4 group. Conclusions. nAG treatment was able to significantly reduce the serum levels of several protein markers of liver fibrosis and also significantly reduced the histological degree of liver fibrosis.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

scholarly journals Associating Serum Iron and Magnesium Levels in Hypertensive and Chronic Periodontitis Patients: Do They have a Link?

2017 ◽  
Vol 8 (2) ◽  
pp. 61-65
Author(s):  
Arati C Koregol ◽  
TJ Jerin Mary ◽  
Shivaraj Warad ◽  
Tejaswini Annam ◽  
Nitesh Kataria ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Serum Iron ◽  
Serum Magnesium ◽  
Control Group ◽  
Tissue Destruction ◽  
Early Effect ◽  
Group Iii ◽  
Group I ◽  
Blue Reaction ◽  
And Control

ABSTRACT Introduction Micronutrients have been extensively studied in recent years; of these magnesium and iron are essential for a variety of physiologic functions. Activation of neutrophils is an early effect of hypomagnesemia, which, in turn, causes periodontal tissue destruction. Since magnesium also influences blood pressure regulation by vascular tone and reactivity, its altered levels could contribute to the pathophysiology of hypertension. Iron plays a key role in the formation of reactive oxygen species that causes peroxidative damage to tissues. Aims and objectives The present study is designed to estimate the levels of serum iron and magnesium in healthy, chronic periodontitis patients with and without hypertension. Materials and methods This study includes 90 subjects, age ranging from 18 to 65 years. They were categorized into three groups as group I: 30 periodontally healthy, group II: 30 chronic periodontitis, and group III: 30 chronic periodontitis with hypertension. Serum magnesium levels were determined using the modified xylidyl blue reaction method and serum iron levels using ferrozine method without deproteinization via photometry. Results Biochemical parameters showed mean serum iron levels in chronic periodontitis (59.17) to be significantly reduced as compared with the control group (76.90) and slightly increased in the chronic periodontitis with hypertension group (69.63). Serum magnesium levels were found to be significantly lower in the hypertensive group as compared with the chronic periodontitis and control groups. Conclusion Iron and magnesium have a link in the pathophysiology of periodontitis. Serum iron and serum magnesium levels were closely linked to periodontitis and hypertension. How to cite this article Kalburgi NB, Koregol AC, Mary TJJ, Warad S, Annam T, Kataria N. Associating Serum Iron and Magnesium Levels in Hypertensive and Chronic Periodontitis Patients: Do They have a Link? J Health Sci Res 2017;8(2):61-65.

scholarly journals Hematological Parameters in Pregnant Women with Special Reference to Iron

2021 ◽  
pp. 137-142
Author(s):  
Shubhranshu Shekhar
Keyword(s):  
Pregnant Women ◽  
Hematological Parameters ◽  
First Trimester ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Cross Sectional ◽  
Hematological Indices ◽  
Group I ◽  
Significant Difference

Several biological factors, particularly haematological, are physiologically altered during normal pregnancy. Biologists and doctors who are aware of these changes in the maternal body can screen for potential abnormalities. The aim of this research is to find healthy pregnant women's reference values. This was a cross-sectional research of pregnant women who attended an antenatal clinic at Sree Balaji Medical College, with anaemic and non-anemic pregnant women. Pregnant women were categorized into three groups -Group I - First Trimester (50 cases); Group II - Second Trimester (50 cases) and Group III - Third Trimester (72 cases) while non­ pregnant women formed the fourth group (30 cases). A statistically significant difference between the pregnant women and control group was noted (p < 0.05) for all the hematological parameters: red blood cells, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, leukocytes, neutrophils, basophils, eosinophils, lymphocytes, monocytes, platelets and mean platelet volume. The present study provides additional baseline data for basic hematological parameters in healthy pregnant women and concluded that pregnancy in women has the tendency to alter some hematological indices.

Effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) on iron absorption in rats

2004 ◽  
Vol 20 (6-10) ◽  
pp. 119-122 ◽  
Author(s):  
Mehmet Akdogan ◽  
Fatih Gultekin ◽  
Mustafa Yontem
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Mentha Piperita ◽  
Control Group ◽  
Albino Rats ◽  
Mentha Spicata ◽  
Group Iii ◽  
Group I ◽  
Wistar Albino Rats ◽  
Iron Binding Capacity

Aim: The effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) teas, which contain different phenol compounds, on iron metabolism was studied. These teas grow in different areas of the city of Isparta, Turkey. These herbals were given to the rats in tea. Methods: Forty-eight male Wistar albino rats weighing 200-250 g were used for this study. The rats were divided into four groups of 12 animals: Group I received no herbal tea (control group); Group II received 20 g/L M. piperita tea; Group III received 20 g/L M. spicata tea; Group IV received 40 g/L M. spicata tea. Herbal teas were prepared daily and provided at all times to the rats over 30 days as drinking water. Results: M. piperita tea caused a decrease in serum iron and ferritin levels P <0.05), and caused an increase in unsaturated iron-binding capacity (UIBC) P <0.01). M. spicata tea caused no significant change in serum iron, ferritin levels and UIBC P <0.05). Conclusion: Both herbal teas inhibited Fe absorption. Inhibition caused by M. spicata tea was dose dependent. Therefore, when drinking these teas, their effect should be considered, especially for children and anemic patients.

scholarly journals Evaluation of Acute Toxicity of Ethanol Extract of Pirdot Leaf (Saurauia vulcani Korth.) in Rats

2020 ◽  
Vol 3 (2) ◽  
pp. 13-18
Author(s):  
Nerly Juli Pranita Simanjuntak ◽  
Rosidah ◽  
Yuandani
Keyword(s):  
Acute Toxicity ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Female Rats ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin

Traditionally pirdot leaves are used to treat various diseases. The purpose of this study was to determine determine the potential for acute toxicity of ethanolic extract of pirdot leaf (Saurauia vulcani Korth.) with value LD50 and hematological Parameters in rats. The acute toxicity of ethanolic extract of pirdot leaf was evaluated by OECD guidelines. The number of animals used in this research were 15 female rats. The control group was given Na CMC 0.5%, the treatment groups were given ethanolic extract of pirdot leaves with doses 2000 and 5000 mg/kg bw. The results showed that ethanolic extract of pirdot leaves with doses of 2000 and 5000 mg/kg bw did not show any toxicity signs. There was no mortality was observe. The ethanolic extract of pirdot leaves did not cause any changes in hematological parameters, these include red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet, white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils levels as compared to normal control (P>0.05). It was estimated that LD50 of ethanolic extract of pirdot leaves was higher than 5000 mg/kg bw and the extract were practically non-toxic. The ethanolic extract of pirdot leaves did not cause any toxic effect on hematological parameters.

Investigation of polyphenol profile, antioxidant activity and hepatoprotective potential of Aconogonon alpinum (All.) Schur roots

2020 ◽  
Vol 18 (1) ◽  
pp. 516-536 ◽  
Author(s):  
Muhammad Zakryya Khan ◽  
Muhammad Imran Shabbir ◽  
Zafeer Saqib ◽  
Syed Aneel Gilani ◽  
Naqeeb Ullah Jogezai ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liver Toxicity ◽  
Serum Levels ◽  
Vital Role ◽  
Control Group ◽  
Histopathological Analysis ◽  
Dependent Manner ◽  
Group Iii ◽  
Group I ◽  
Polyphenol Profile

AbstractLiver plays vital role in detoxification of exogenous and endogenous chemicals. These chemicals as well as oxidative stress may cause liver disorders. This study was aimed to evaluate the hepatoprotective effects of various fractions of Aconogonon alpinum methanolic extract against carbon tetrachloride (CCl4)-induced liver toxicity in mice. First, hepatoprotective potential of various fractions of A. alpinum was assessed and then antioxidant activity and profiling of polyphenolic compounds were assessed. A total of 78 male albino mice (BALB/c) were randomly divided into 13 groups (n = 6); Group I (normal control), Group II (CCl4 only), Group III (CCl4 + silymarin 100 mg/kg) and Groups IV–XIII (CCl4 + various fractions [200 and 400 mg/kg]). Hepatic biochemistry and liver injury were assessed by analysis of serum levels of hepatic enzymes and histopathological analysis, respectively. Results showed that polar fractions (ethyl acetate, n-butanol and aqueous fractions) exhibited highly significant (P < 0.01) reduction in increased level of liver biochemical parameters in a dose-dependent manner with consistent histopathological findings. Likewise, these fractions revealed strong antioxidant potential and polyphenolic compound contents. In conclusion, the present work has revealed promising antioxidant activity, polyphenolic profiling and potential hepatoprotective efficacy. Thus, the significant results unveil the study as a step forward towards evidence-based phytomedicine.

scholarly journals The Effects of Armillarisin A on Serum IL-1β and IL-4 and in Treating Ulcerative Colitis

2014 ◽  
Vol 72 (1) ◽  
pp. 103-106 ◽  
Author(s):  
Ping Wu ◽  
Yonggao Guo ◽  
Fangyuan Jia ◽  
Xiuli Wang
Keyword(s):  
Ulcerative Colitis ◽  
Treatment Group ◽  
Therapeutic Efficacy ◽  
Therapy Group ◽  
Serum Levels ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Control Group Group

Abstract To study the therapeutic effect of Armillarisin A on patients with ulcerative colitis (UC) and on serum IL-1β and IL-4, sixty patients with UC were randomly divided into three groups: Armillarisin A treatment group (Group I), Armillarisin-combined hormone therapy group (Group II), and hormones treatment as the control group (Group III). Patients in Group I received Armillarisin A 10 mg enema in 100 ml saline. Patients in Group II received Armillarisin A 10 mg and dexamethasone 5 mg enema in 100 ml saline. Patients in Group III received only dexamethasone 5 mg enema in 100 ml saline. The therapeutic efficacy and serum levels of IL-4 and IL-1β were observed. After 4 week treatment, the total effective rates were 90.0 % in Group I and 95.0 % in Group II. Both are higher than it in control group, which was 70.0 %. The serum levels of IL-4 in Groups I and II were significantly higher than it in control group. Compared to IL-4 levels before treatment, the levels of IL-4 after treatment were significantly higher in both Groups I and II. The serum levels of IL-11β were significantly decreased in Groups I and II in comparison to it in control group. Compared to the levels of IL-1β before treatment, the levels of IL-1β were significantly decreased. Armillarisin A shows a significant effect in treating UC. It helps increase IL-4 and lower IL-1β and the mechanism may be related to the body’s immunity regulation.

scholarly journals Increased Zinc and Albumin but Lowered Copper in Children with Transfusion-dependent Thalassemia

2020 ◽  
Author(s):  
Zainab Alhillawi ◽  
Hussein Al-Hakeim ◽  
Shatha Moustafa ◽  
Michael Maes
Keyword(s):  
Iron Overload ◽  
Mean Corpuscular Volume ◽  
Serum Zinc ◽  
Serum Levels ◽  
Serum Copper ◽  
Mean Corpuscular Hemoglobin ◽  
Compensatory Mechanism ◽  
Healthy Children ◽  
Corpuscular Volume ◽  
Corpuscular Hemoglobin

Measurements of copper and zinc in transfusion-dependent thalassemia (TDT) show contradictory results.Aim of the study: To examine serum levels of these minerals in TDT in relation to iron overload indices and erythron variables. Methods: This study recruited 60 children with TDT and 30 healthy children aged 3-12 years old.Results: Zinc was significantly higher in TDT children than in control children, whilst copper and the copper to zinc ratio were significantly lowered in TDT. Serum zinc was significantly associated with the number of blood transfusions and iron overload variables (including serum iron and TS%) and negatively with erythron variables (including hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin). Serum copper was significantly and negatively associated with the same iron overload and erythron variables. The copper to zinc ratio was significantly correlated with iron, TS%, ferritin, hemoglobin, mean corpuscular volume, and mean corpuscular hemoglobin. Albumin levels were significantly higher in TDT children than in control children. Conclusion: Our results suggest that the increase in zinc in children with TDT may be explained by iron loading anemia and hemolysis and the consequent shedding of high amounts of intracellular zinc into the plasma. Increased albumin levels and treatment with Desferral may further contribute towards higher zinc levels in TDT. We suggest that the elevations in zinc in TDT are a compensatory mechanism protecting against infection, inflammation, and oxidative stress. Previous proposals for prophylactic use of zinc supplements in TDT may not be warranted.

scholarly journals Alteration in MDA, GSH level and hematological changes due to thiamine deficiency in Mus musculus

2018 ◽  
Vol 11 (4) ◽  
pp. 321-325
Author(s):  
Anupama Sharma ◽  
Renu Bist
Keyword(s):  
Cell Volume ◽  
Thiamine Deficiency ◽  
Hematological Parameters ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Deficient Diet ◽  
Swiss Albino Mice ◽  
Group Iii ◽  
Albino Mice

Abstract It is known that thiamine deficiency may lead to Alzheimer’s diseases in humans. The present study has thus been conducted to understand the role of thiamine deficiency with respect to alteration in the peripheral blood of Swiss albino mice. For this purpose, adult Swiss albino mice (6–8 week old) were divided into three groups. The first group was control; the second (group II) and the third group (group III) were made thiamine deficient for 08 and 10 days respectively. Thiamine deficiency was induced in mice by injecting pyrithiamine (5 µg/10 g bwt) and feeding a thiamine deficient diet. The erythrocytes, leukocytes count, hemoglobin, hematocrit value, mass cell volume, mean corpuscular hemoglobin in blood of mice were determined by hematoanalyzer. Malondialdehyde (MDA) and reduced glutathione (GSH) level was also determined in serum of treated and non-treated groups. A significant reduction in leukocyte and erythrocyte count was observed in both the thiamine deficient groups as compared to control. Levels of hemoglobin and hematocrit value were also declined in the thiamine deficient groups. Enhancement in mass cell volume (MCV) level and decline in mean corpuscular hemoglobin (MCH) levels were observed in both thiamine deficient groups with respect to control. Inter-group comparison of all parameters also showed a significant value at p<0.01. In comparison with the control group, elevation in MDA and decline in GSH level was observed in both thiamine deficient groups which were statistically significant. These data indicate that thiamine deficiency leads to significant alterations in the hematological parameters as well as in MDA and GSH level.

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